                         SEQUENCE LISTING

<110>  Spatial Transcriptomics AB
 
<120>  Method for spatial tagging and analysing nucleic acids in a 
       biological specimen

<130>  20.129188/02

<150>  1619458.1
<151>  2016-11-17

<160>  8     

<170>  PatentIn version 3.5

<210>  1
<211>  1680
<212>  DNA
<213>  Murine leukemia virus

<400>  1
gcttttcccc tcgagcgccc agactgggat tacaccaccc aggcaggtag gaaccaccta       60

gtccactatc gccagttgct cctagcgggt ctccaaaacg cgggcagaag ccccaccaat      120

ttggccaagg taaaagggat aacacaggga cctaatgagt ctccctcagc ctttttagag      180

agactcaagg aggcctatcg caggtacact ccttatgacc ctgaggaccc agggcaagaa      240

accaatgtgt ctatgtcatt catctggcag tctgccccgg atatcgggcg aaagttaggg      300

cggttagaag atttaaagag caagacctta ggagacttag ttagggaggc tgaaaagatc      360

tttaataaac gtgaaacccc ggaagaaaga gaggaacgta tcaggagaga aacagaggaa      420

aaagaagaac gccgtaggac agtggatgag cagaaagaga aagaaaggga ccgcagaaga      480

catagagaga tgagcaagct cttggccact gtagttattg gtcaggaaca ggatagacag      540

gagggagagc ggaagaggcc ccaacttgat aaggatcaat gcgcctactg caaagaaaag      600

gggcactggg ctaaagactg tcccaagaag ccacgagggc cccgaggacc caggccccag      660

acctccctcc tgaccttagg tgactaggga ggtcagggtc aggacccccc ccctgaaccc      720

aggataaccc tcaaagtcgg ggggcaaccc gtcaccttcc tggtagatac tggggcccaa      780

cactccgtgc tgacccaaaa tcctggaccc ctaagtgaca agtctgcctg ggtccaaggg      840

gctactggag gaaagcggta tcgctggacc acggatcgca aagtgcatct agctaccggt      900

aaggtcaccc actctttcct ccatgtacca gactgcccct atcctctgct aggaagagat      960

ttgctgacta aactaaaagc ccaaatccac tttgagggat caggagctca ggttgtggga     1020

ccaatgggac agcccctgca agtgctgacc ctaaacatag aagatgagta tcggctacat     1080

gagacctcaa aagagccgga tgtttctcta gggttcacat ggctttctga ttttcctcag     1140

gcctgggcgg aatccggggg catgggactg gcagttcgcc aagctcctct gatcatacct     1200

ctgaaggcaa cctctacccc cgtgtccata aaacaatacc ccatgtcaca agaagccaga     1260

ctggggatca agccccacat acagagactg ttggaccagg gaatactggt accctgccag     1320

tccccctgga acacgcccct gctacccgtt aagaaaccag ggactaatga ttacaggcct     1380

gtccaagatc tgagagaagt caacaagcgg gtggaagaca tccaccccac cgtgcccaac     1440

ccttacaacc tcttgagcgg gctcccaccg tcccaccagt ggtacactgt gcttgactta     1500

aaggatgcct ttttctgcct gagactccac cccaccagtc agcctctctt cgcctttgag     1560

tggagagacc cagagatggg aatctcagga caattaacct ggaccagact cccacagggt     1620

ttcaaaaaca gtcccaccct gtttgatgag gcactgcaca gagacctagc agacttccgg     1680


<210>  2
<211>  560
<212>  PRT
<213>  Murine leukemia virus


<220>
<221>  misc_feature
<222>  (229)..(229)
<223>  Xaa can be any naturally occurring amino acid

<400>  2

Ala Phe Pro Leu Glu Arg Pro Asp Trp Asp Tyr Thr Thr Gln Ala Gly 
1               5                   10                  15      


Arg Asn His Leu Val His Tyr Arg Gln Leu Leu Leu Ala Gly Leu Gln 
            20                  25                  30          


Asn Ala Gly Arg Ser Pro Thr Asn Leu Ala Lys Val Lys Gly Ile Thr 
        35                  40                  45              


Gln Gly Pro Asn Glu Ser Pro Ser Ala Phe Leu Glu Arg Leu Lys Glu 
    50                  55                  60                  


Ala Tyr Arg Arg Tyr Thr Pro Tyr Asp Pro Glu Asp Pro Gly Gln Glu 
65                  70                  75                  80  


Thr Asn Val Ser Met Ser Phe Ile Trp Gln Ser Ala Pro Asp Ile Gly 
                85                  90                  95      


Arg Lys Leu Gly Arg Leu Glu Asp Leu Lys Ser Lys Thr Leu Gly Asp 
            100                 105                 110         


Leu Val Arg Glu Ala Glu Lys Ile Phe Asn Lys Arg Glu Thr Pro Glu 
        115                 120                 125             


Glu Arg Glu Glu Arg Ile Arg Arg Glu Thr Glu Glu Lys Glu Glu Arg 
    130                 135                 140                 


Arg Arg Thr Val Asp Glu Gln Lys Glu Lys Glu Arg Asp Arg Arg Arg 
145                 150                 155                 160 


His Arg Glu Met Ser Lys Leu Leu Ala Thr Val Val Ile Gly Gln Glu 
                165                 170                 175     


Gln Asp Arg Gln Glu Gly Glu Arg Lys Arg Pro Gln Leu Asp Lys Asp 
            180                 185                 190         


Gln Cys Ala Tyr Cys Lys Glu Lys Gly His Trp Ala Lys Asp Cys Pro 
        195                 200                 205             


Lys Lys Pro Arg Gly Pro Arg Gly Pro Arg Pro Gln Thr Ser Leu Leu 
    210                 215                 220                 


Thr Leu Gly Asp Xaa Gly Gly Gln Gly Gln Asp Pro Pro Pro Glu Pro 
225                 230                 235                 240 


Arg Ile Thr Leu Lys Val Gly Gly Gln Pro Val Thr Phe Leu Val Asp 
                245                 250                 255     


Thr Gly Ala Gln His Ser Val Leu Thr Gln Asn Pro Gly Pro Leu Ser 
            260                 265                 270         


Asp Lys Ser Ala Trp Val Gln Gly Ala Thr Gly Gly Lys Arg Tyr Arg 
        275                 280                 285             


Trp Thr Thr Asp Arg Lys Val His Leu Ala Thr Gly Lys Val Thr His 
    290                 295                 300                 


Ser Phe Leu His Val Pro Asp Cys Pro Tyr Pro Leu Leu Gly Arg Asp 
305                 310                 315                 320 


Leu Leu Thr Lys Leu Lys Ala Gln Ile His Phe Glu Gly Ser Gly Ala 
                325                 330                 335     


Gln Val Val Gly Pro Met Gly Gln Pro Leu Gln Val Leu Thr Leu Asn 
            340                 345                 350         


Ile Glu Asp Glu Tyr Arg Leu His Glu Thr Ser Lys Glu Pro Asp Val 
        355                 360                 365             


Ser Leu Gly Phe Thr Trp Leu Ser Asp Phe Pro Gln Ala Trp Ala Glu 
    370                 375                 380                 


Ser Gly Gly Met Gly Leu Ala Val Arg Gln Ala Pro Leu Ile Ile Pro 
385                 390                 395                 400 


Leu Lys Ala Thr Ser Thr Pro Val Ser Ile Lys Gln Tyr Pro Met Ser 
                405                 410                 415     


Gln Glu Ala Arg Leu Gly Ile Lys Pro His Ile Gln Arg Leu Leu Asp 
            420                 425                 430         


Gln Gly Ile Leu Val Pro Cys Gln Ser Pro Trp Asn Thr Pro Leu Leu 
        435                 440                 445             


Pro Val Lys Lys Pro Gly Thr Asn Asp Tyr Arg Pro Val Gln Asp Leu 
    450                 455                 460                 


Arg Glu Val Asn Lys Arg Val Glu Asp Ile His Pro Thr Val Pro Asn 
465                 470                 475                 480 


Pro Tyr Asn Leu Leu Ser Gly Leu Pro Pro Ser His Gln Trp Tyr Thr 
                485                 490                 495     


Val Leu Asp Leu Lys Asp Ala Phe Phe Cys Leu Arg Leu His Pro Thr 
            500                 505                 510         


Ser Gln Pro Leu Phe Ala Phe Glu Trp Arg Asp Pro Glu Met Gly Ile 
        515                 520                 525             


Ser Gly Gln Leu Thr Trp Thr Arg Leu Pro Gln Gly Phe Lys Asn Ser 
    530                 535                 540                 


Pro Thr Leu Phe Asp Glu Ala Leu His Arg Asp Leu Ala Asp Phe Arg 
545                 550                 555                 560 


<210>  3
<211>  100
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Probe 1


<220>
<221>  misc_feature
<222>  (72)..(73)
<223>  n is a, c, g, t or u

<220>
<221>  misc_feature
<222>  (76)..(77)
<223>  n is a, c, g, t or u

<220>
<221>  misc_feature
<222>  (100)..(100)
<223>  n is a, c, g, t or u

<400>  3
uuuuugactc gtaatacgac tcactatagg gacacgacgc tcttccgatc tgtccgatat       60

gattgccgcw snnwsnnvtt tttttttttt ttttttttvn                            100


<210>  4
<211>  35
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Ligation adaptor

<400>  4
agatcggaag agcacacgtc tgaactccag tcacc                                  35


<210>  5
<211>  30
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Second reverse transcription primer

<400>  5
tgactggagt tcagacgtgt gctcttccga                                        30


<210>  6
<211>  57
<212>  DNA
<213>  Artificial sequence

<220>
<223>  PCR primer InPE1.0

<400>  6
atgatacggc gaccaccgag atctacactc tttccctaca cgacgctctt ccgatct          57


<210>  7
<211>  34
<212>  DNA
<213>  Artificial sequence

<220>
<223>  PCR primer InPE2.0

<400>  7
gtgactggag ttcagacgtg tgctcttccg atct                                   34


<210>  8
<211>  42
<212>  DNA
<213>  Artificial sequence

<220>
<223>  PCR Index primer


<220>
<221>  misc_feature
<222>  (24)..(29)
<223>  n is a, c, g, or t

<400>  8
aagcagaaga cggcatacga gatnnnnnng tgactggagt tc                          42


