                          б

<110>  ũƼŹɷ޹˾
       ũ＼޹˾
 
<120>  ɱ浰ϼ濹Եķ

<130>  DBNBC115

<160>  4     

<170>  PatentIn version 3.5

<210>  1
<211>  634
<212>  PRT
<213>  ˹

<220>
<223>  Cry2Ab

<400>  1

Met Asp Asn Ser Val Leu Asn Ser Gly Arg Thr Thr Ile Cys Asp Ala 
1               5                   10                  15      


Tyr Asn Val Ala Ala His Asp Pro Phe Ser Phe Gln His Lys Ser Leu 
            20                  25                  30          


Asp Thr Val Gln Lys Glu Trp Thr Glu Trp Lys Lys Asn Asn His Ser 
        35                  40                  45              


Leu Tyr Leu Asp Pro Ile Val Gly Thr Val Ala Ser Phe Leu Leu Lys 
    50                  55                  60                  


Lys Val Gly Ser Leu Val Gly Lys Arg Ile Leu Ser Glu Leu Arg Asn 
65                  70                  75                  80  


Leu Ile Phe Pro Ser Gly Ser Thr Asn Leu Met Gln Asp Ile Leu Arg 
                85                  90                  95      


Glu Thr Glu Lys Phe Leu Asn Gln Arg Leu Asn Thr Asp Thr Leu Ala 
            100                 105                 110         


Arg Val Asn Ala Glu Leu Thr Gly Leu Gln Ala Asn Val Glu Glu Phe 
        115                 120                 125             


Asn Arg Gln Val Asp Asn Phe Leu Asn Pro Asn Arg Asn Ala Val Pro 
    130                 135                 140                 


Leu Ser Ile Thr Ser Ser Val Asn Thr Met Gln Gln Leu Phe Leu Asn 
145                 150                 155                 160 


Arg Leu Pro Gln Phe Gln Met Gln Gly Tyr Gln Leu Leu Leu Leu Pro 
                165                 170                 175     


Leu Phe Ala Gln Ala Ala Asn Leu His Leu Ser Phe Ile Arg Asp Val 
            180                 185                 190         


Ile Leu Asn Ala Asp Glu Trp Gly Ile Ser Ala Ala Thr Leu Arg Thr 
        195                 200                 205             


Tyr Arg Asp Tyr Leu Lys Asn Tyr Thr Arg Asp Tyr Ser Asn Tyr Cys 
    210                 215                 220                 


Ile Asn Thr Tyr Gln Ser Ala Phe Lys Gly Leu Asn Thr Arg Leu His 
225                 230                 235                 240 


Asp Met Leu Glu Phe Arg Thr Tyr Met Phe Leu Asn Val Phe Glu Tyr 
                245                 250                 255     


Val Ser Ile Trp Ser Leu Phe Lys Tyr Gln Ser Leu Leu Val Ser Ser 
            260                 265                 270         


Gly Ala Asn Leu Tyr Ala Ser Gly Ser Gly Pro Gln Gln Thr Gln Ser 
        275                 280                 285             


Phe Thr Ser Gln Asp Trp Pro Phe Leu Tyr Ser Leu Phe Gln Val Asn 
    290                 295                 300                 


Ser Asn Tyr Val Leu Asn Gly Phe Ser Gly Ala Arg Leu Ser Asn Thr 
305                 310                 315                 320 


Phe Pro Asn Ile Val Gly Leu Pro Gly Ser Thr Thr Thr His Ala Leu 
                325                 330                 335     


Leu Ala Ala Arg Val Asn Tyr Ser Gly Gly Ile Ser Ser Gly Asp Ile 
            340                 345                 350         


Gly Ala Ser Pro Phe Asn Gln Asn Phe Asn Cys Ser Thr Phe Leu Pro 
        355                 360                 365             


Pro Leu Leu Thr Pro Phe Val Arg Ser Trp Leu Asp Ser Gly Ser Asp 
    370                 375                 380                 


Arg Glu Gly Val Ala Thr Val Thr Asn Trp Gln Thr Glu Ser Phe Glu 
385                 390                 395                 400 


Thr Thr Leu Gly Leu Arg Ser Gly Ala Phe Thr Ala Arg Gly Asn Ser 
                405                 410                 415     


Asn Tyr Phe Pro Asp Tyr Phe Ile Arg Asn Ile Ser Gly Val Pro Leu 
            420                 425                 430         


Val Val Arg Asn Glu Asp Leu Arg Arg Pro Leu His Tyr Asn Glu Ile 
        435                 440                 445             


Arg Asn Ile Ala Ser Pro Ser Gly Thr Pro Gly Gly Ala Arg Ala Tyr 
    450                 455                 460                 


Met Val Ser Val His Asn Arg Lys Asn Asn Ile His Ala Val His Glu 
465                 470                 475                 480 


Asn Gly Ser Met Ile His Leu Ala Pro Asn Asp Tyr Thr Gly Phe Thr 
                485                 490                 495     


Ile Ser Pro Ile His Ala Thr Gln Val Asn Asn Gln Thr Arg Thr Phe 
            500                 505                 510         


Ile Ser Glu Lys Phe Gly Asn Gln Gly Asp Ser Leu Arg Phe Glu Gln 
        515                 520                 525             


Asn Asn Thr Thr Ala Arg Tyr Thr Leu Arg Gly Asn Gly Asn Ser Tyr 
    530                 535                 540                 


Asn Leu Tyr Leu Arg Val Ser Ser Ile Gly Asn Ser Thr Ile Arg Val 
545                 550                 555                 560 


Thr Ile Asn Gly Arg Val Tyr Thr Ala Thr Asn Val Asn Thr Thr Thr 
                565                 570                 575     


Asn Asn Asp Gly Val Asn Asp Asn Gly Ala Arg Phe Ser Asp Ile Asn 
            580                 585                 590         


Ile Gly Asn Val Val Ala Ser Ser Asn Ser Asp Val Pro Leu Asp Ile 
        595                 600                 605             


Asn Val Thr Leu Asn Ser Gly Thr Gln Phe Asp Leu Met Asn Ile Met 
    610                 615                 620                 


Leu Val Pro Thr Asn Ile Ser Pro Leu Tyr 
625                 630                 


<210>  2
<211>  818
<212>  PRT
<213>  ˹

<220>
<223>  Cry1Ab

<400>  2

Met Asp Asn Asn Pro Asn Ile Asn Glu Cys Ile Pro Tyr Asn Cys Leu 
1               5                   10                  15      


Ser Asn Pro Glu Val Glu Val Leu Gly Gly Glu Arg Ile Glu Thr Gly 
            20                  25                  30          


Tyr Thr Pro Ile Asp Ile Ser Leu Ser Leu Thr Gln Phe Leu Leu Ser 
        35                  40                  45              


Glu Phe Val Pro Gly Ala Gly Phe Val Leu Gly Leu Val Asp Ile Ile 
    50                  55                  60                  


Trp Gly Ile Phe Gly Pro Ser Gln Trp Asp Ala Phe Leu Val Gln Ile 
65                  70                  75                  80  


Glu Gln Leu Ile Asn Gln Arg Ile Glu Glu Phe Ala Arg Asn Gln Ala 
                85                  90                  95      


Ile Ser Arg Leu Glu Gly Leu Ser Asn Leu Tyr Gln Ile Tyr Ala Glu 
            100                 105                 110         


Ser Phe Arg Glu Trp Glu Ala Asp Pro Thr Asn Pro Ala Leu Arg Glu 
        115                 120                 125             


Glu Met Arg Ile Gln Phe Asn Asp Met Asn Ser Ala Leu Thr Thr Ala 
    130                 135                 140                 


Ile Pro Leu Phe Ala Val Gln Asn Tyr Gln Val Pro Leu Leu Ser Val 
145                 150                 155                 160 


Tyr Val Gln Ala Ala Asn Leu His Leu Ser Val Leu Arg Asp Val Ser 
                165                 170                 175     


Val Phe Gly Gln Arg Trp Gly Phe Asp Ala Ala Thr Ile Asn Ser Arg 
            180                 185                 190         


Tyr Asn Asp Leu Thr Arg Leu Ile Gly Asn Tyr Thr Asp His Ala Val 
        195                 200                 205             


Arg Trp Tyr Asn Thr Gly Leu Glu Arg Val Trp Gly Pro Asp Ser Arg 
    210                 215                 220                 


Asp Trp Ile Arg Tyr Asn Gln Phe Arg Arg Glu Leu Thr Leu Thr Val 
225                 230                 235                 240 


Leu Asp Ile Val Ser Leu Phe Pro Asn Tyr Asp Ser Arg Thr Tyr Pro 
                245                 250                 255     


Ile Arg Thr Val Ser Gln Leu Thr Arg Glu Ile Tyr Thr Asn Pro Val 
            260                 265                 270         


Leu Glu Asn Phe Asp Gly Ser Phe Arg Gly Ser Ala Gln Gly Ile Glu 
        275                 280                 285             


Gly Ser Ile Arg Ser Pro His Leu Met Asp Ile Leu Asn Ser Ile Thr 
    290                 295                 300                 


Ile Tyr Thr Asp Ala His Arg Gly Glu Tyr Tyr Trp Ser Gly His Gln 
305                 310                 315                 320 


Ile Met Ala Ser Pro Val Gly Phe Ser Gly Pro Glu Phe Thr Phe Pro 
                325                 330                 335     


Leu Tyr Gly Thr Met Gly Asn Ala Ala Pro Gln Gln Arg Ile Val Ala 
            340                 345                 350         


Gln Leu Gly Gln Gly Val Tyr Arg Thr Leu Ser Ser Thr Leu Tyr Arg 
        355                 360                 365             


Arg Pro Phe Asn Ile Gly Ile Asn Asn Gln Gln Leu Ser Val Leu Asp 
    370                 375                 380                 


Gly Thr Glu Phe Ala Tyr Gly Thr Ser Ser Asn Leu Pro Ser Ala Val 
385                 390                 395                 400 


Tyr Arg Lys Ser Gly Thr Val Asp Ser Leu Asp Glu Ile Pro Pro Gln 
                405                 410                 415     


Asn Asn Asn Val Pro Pro Arg Gln Gly Phe Ser His Arg Leu Ser His 
            420                 425                 430         


Val Ser Met Phe Arg Ser Gly Phe Ser Asn Ser Ser Val Ser Ile Ile 
        435                 440                 445             


Arg Ala Pro Met Phe Ser Trp Ile His Arg Ser Ala Glu Phe Asn Asn 
    450                 455                 460                 


Ile Ile Pro Ser Ser Gln Ile Thr Gln Ile Pro Leu Thr Lys Ser Thr 
465                 470                 475                 480 


Asn Leu Gly Ser Gly Thr Ser Val Val Lys Gly Pro Gly Phe Thr Gly 
                485                 490                 495     


Gly Asp Ile Leu Arg Arg Thr Ser Pro Gly Gln Ile Ser Thr Leu Arg 
            500                 505                 510         


Val Asn Ile Thr Ala Pro Leu Ser Gln Arg Tyr Arg Val Arg Ile Arg 
        515                 520                 525             


Tyr Ala Ser Thr Thr Asn Leu Gln Phe His Thr Ser Ile Asp Gly Arg 
    530                 535                 540                 


Pro Ile Asn Gln Gly Asn Phe Ser Ala Thr Met Ser Ser Gly Ser Asn 
545                 550                 555                 560 


Leu Gln Ser Gly Ser Phe Arg Thr Val Gly Phe Thr Thr Pro Phe Asn 
                565                 570                 575     


Phe Ser Asn Gly Ser Ser Val Phe Thr Leu Ser Ala His Val Phe Asn 
            580                 585                 590         


Ser Gly Asn Glu Val Tyr Ile Asp Arg Ile Glu Phe Val Pro Ala Glu 
        595                 600                 605             


Val Thr Phe Glu Ala Glu Tyr Asp Leu Glu Arg Ala Gln Lys Ala Val 
    610                 615                 620                 


Asn Glu Leu Phe Thr Ser Ser Asn Gln Ile Gly Leu Lys Thr Asp Val 
625                 630                 635                 640 


Thr Asp Tyr His Ile Asp Gln Val Ser Asn Leu Val Glu Cys Leu Ser 
                645                 650                 655     


Asp Glu Phe Cys Leu Asp Glu Lys Lys Glu Leu Ser Glu Lys Val Lys 
            660                 665                 670         


His Ala Lys Arg Leu Ser Asp Glu Arg Asn Leu Leu Gln Asp Pro Asn 
        675                 680                 685             


Phe Arg Gly Ile Asn Arg Gln Leu Asp Arg Gly Trp Arg Gly Ser Thr 
    690                 695                 700                 


Asp Ile Thr Ile Gln Gly Gly Asp Asp Val Phe Lys Glu Asn Tyr Val 
705                 710                 715                 720 


Thr Leu Leu Gly Thr Phe Asp Glu Cys Tyr Pro Thr Tyr Leu Tyr Gln 
                725                 730                 735     


Lys Ile Asp Glu Ser Lys Leu Lys Ala Tyr Thr Arg Tyr Gln Leu Arg 
            740                 745                 750         


Gly Tyr Ile Glu Asp Ser Gln Asp Leu Glu Ile Tyr Leu Ile Arg Tyr 
        755                 760                 765             


Asn Ala Lys His Glu Thr Val Asn Val Pro Gly Thr Gly Ser Leu Trp 
    770                 775                 780                 


Pro Leu Ser Ala Pro Ser Pro Ile Gly Lys Cys Ala His His Ser His 
785                 790                 795                 800 


His Phe Ser Leu Asp Ile Asp Val Gly Cys Thr Asp Leu Asn Glu Asp 
                805                 810                 815     


Phe Arg 
        


<210>  3
<211>  1905
<212>  DNA
<213>  ˹

<220>
<223>  Cry2Ab

<400>  3
atggataact cagttctgaa ttcgggccgt accacgattt gcgatgccta taatgtcgcg       60

gcccatgacc cgtttagctt ccaacacaaa tctctggata ccgtgcagaa agaatggacg      120

gaatggaaga aaaacaatca tagcctgtac ctggacccga ttgtgggcac cgttgcgagc      180

tttctgctga agaaagtggg ctctctggtc ggtaaacgta ttctgagtga actgcgcaat      240

ctgatctttc cgtcaggttc gaccaacctg atgcaagata tcctgcgtga aacggaaaaa      300

ttcctgaacc agcgtctgaa taccgacacg ctggcgcgcg ttaacgccga actgaccggt      360

ctgcaagcaa acgtggaaga atttaatcgt caggttgata acttcctgaa cccgaatcgc      420

aacgctgtcc cgctgtctat taccagctct gtgaatacga tgcagcaact gtttctgaac      480

cgcctgccgc agttccaaat gcagggctat caactgctgc tgctgccgct gtttgcgcag      540

gcagctaatc tgcatctgag cttcattcgt gatgtgatcc tgaacgccga cgaatggggt      600

atttctgcgg ccaccctgcg tacgtatcgc gattacctga aaaattatac ccgcgactat      660

tccaactact gcatcaatac gtaccagtca gcatttaaag gcctgaatac ccgtctgcac      720

gatatgctgg aattccgcac ctatatgttt ctgaacgtgt tcgaatacgt tagcatttgg      780

tctctgttta aatatcagag cctgctggtt agctccggtg caaacctgta cgctagcggc      840

tctggtccgc agcaaaccca aagtttcacg tcccaggatt ggccgtttct gtattcactg      900

ttccaggtca attcgaacta cgtgctgaat ggctttagtg gtgcccgtct gtccaacacc      960

ttcccgaata tcgtgggtct gccgggttca accacgaccc acgcactgct ggcagctcgt     1020

gttaactatt cgggcggtat ttcatcgggc gatatcggtg cctcgccgtt taatcagaac     1080

ttcaattgta gcacctttct gccgccgctg ctgacgccgt tcgttcgtag ctggctggat     1140

agtggttccg accgtgaagg tgtcgcaacc gtgacgaatt ggcagaccga atcttttgaa     1200

acgaccctgg gcctgcgtag tggtgcattc accgctcgcg gcaactccaa ctacttcccg     1260

gattacttca tccgtaacat cagcggcgtg ccgctggttg tgcgcaacga agacctgcgt     1320

cgcccgctgc attataacga aattcgcaat atcgcctcac cgtcgggcac cccgggcggt     1380

gcacgtgcat acatggtttc tgtccacaac cgcaaaaaca atattcatgc ggtccacgaa     1440

aatggcagta tgatccatct ggccccgaac gattataccg gttttacgat ttccccgatc     1500

cacgcaaccc aagtgaacaa tcagacccgt acgtttattt cagaaaaatt cggcaatcag     1560

ggtgattcgc tgcgctttga acagaacaat acgaccgctc gttataccct gcgcggcaac     1620

ggtaatagtt ataacctgta cctgcgtgtt agctctatcg gcaattccac cattcgtgtt     1680

acgatcaacg gtcgcgtcta caccgcgacg aacgtgaata cgaccacgaa caatgatggc     1740

gtgaacgaca atggtgcacg ctttagcgat attaacatcg gcaatgtcgt ggctagttcc     1800

aatagcgatg ttccgctgga cattaacgtc accctgaatt ctggtacgca gtttgacctg     1860

atgaatatta tgctggtgcc gaccaacatc agcccgctgt attaa                     1905


<210>  4
<211>  2457
<212>  DNA
<213>  ˹

<220>
<223>  Cry1Ab

<400>  4
atggacaaca acccaaacat caacgagtgc atcccgtaca actgcctcag caaccctgag       60

gtcgaggtgc tcggcggtga gcgcatcgag accggttaca cccccatcga catctccctc      120

tccctcacgc agttcctgct cagcgagttc gtgccaggcg ctggcttcgt cctgggcctc      180

gtggacatca tctggggcat ctttggcccc tcccagtggg acgccttcct ggtgcaaatc      240

gagcagctca tcaaccagag gatcgaggag ttcgccagga accaggccat cagccgcctg      300

gagggcctca gcaacctcta ccaaatctac gctgagagct tccgcgagtg ggaggccgac      360

cccactaacc cagctctccg cgaggagatg cgcatccagt tcaacgacat gaacagcgcc      420

ctgaccaccg ccatcccact cttcgccgtc cagaactacc aagtcccgct cctgtccgtg      480

tacgtccagg ccgccaacct gcacctcagc gtgctgaggg acgtcagcgt gtttggccag      540

aggtggggct tcgacgccgc caccatcaac agccgctaca acgacctcac caggctgatc      600

ggcaactaca ccgaccacgc tgtccgctgg tacaacactg gcctggagcg cgtctggggc      660

cctgattcta gagactggat tcgctacaac cagttcaggc gcgagctgac cctcaccgtc      720

ctggacattg tgtccctctt cccgaactac gactcccgca cctacccgat ccgcaccgtg      780

tcccaactga cccgcgaaat ctacaccaac cccgtcctgg agaacttcga cggtagcttc      840

aggggcagcg cccagggcat cgagggctcc atcaggagcc cacacctgat ggacatcctc      900

aacagcatca ctatctacac cgatgcccac cgcggcgagt actactggtc cggccaccag      960

atcatggcct ccccggtcgg cttcagcggc cccgagttta cctttcctct ctacggcacg     1020

atgggcaacg ccgctccaca acaacgcatc gtcgctcagc tgggccaggg cgtctaccgc     1080

accctgagct ccaccctgta ccgcaggccc ttcaacatcg gtatcaacaa ccagcagctg     1140

tccgtcctgg atggcactga gttcgcctac ggcacctcct ccaacctgcc ctccgctgtc     1200

taccgcaaga gcggcacggt ggattccctg gacgagatcc caccacagaa caacaatgtg     1260

ccccccaggc agggtttttc ccacaggctc agccacgtgt ccatgttccg ctccggcttc     1320

agcaactcgt ccgtgagcat catcagagct cctatgttct cctggattca tcgcagcgcg     1380

gagttcaaca atatcattcc gtcctcccaa atcacccaaa tccccctcac caagtccacc     1440

aacctgggca gcggcacctc cgtggtgaag ggcccaggct tcacgggcgg cgacatcctg     1500

cgcaggacct ccccgggcca gatcagcacc ctccgcgtca acatcaccgc tcccctgtcc     1560

cagaggtacc gcgtcaggat tcgctacgct agcaccacca acctgcaatt ccacacctcc     1620

atcgacggca ggccgatcaa tcagggtaac ttctccgcca ccatgtccag cggcagcaac     1680

ctccaatccg gcagcttccg caccgtgggt ttcaccaccc ccttcaactt ctccaacggc     1740

tccagcgttt tcaccctgag cgcccacgtg ttcaattccg gcaatgaggt gtacattgac     1800

cgcattgagt tcgtgccagc cgaggtcacc ttcgaagccg agtacgacct ggagagagcc     1860

cagaaggctg tcaatgagct cttcacgtcc agcaatcaga tcggcctgaa gaccgacgtc     1920

actgactacc acatcgacca agtctccaac ctcgtggagt gcctctccga tgagttctgc     1980

ctcgacgaga agaaggagct gtccgagaag gtgaagcatg ccaagcgtct cagcgacgag     2040

aggaatctcc tccaggaccc caatttccgc ggcatcaaca ggcagctcga ccgcggctgg     2100

cgcggcagca ccgacatcac gatccagggc ggcgacgatg tgttcaagga gaactacgtg     2160

actctcctgg gcactttcga cgagtgctac cctacctact tgtaccagaa gatcgatgag     2220

tccaagctca aggcttacac tcgctaccag ctccgcggct acatcgaaga cagccaagac     2280

ctcgagattt acctgatccg ctacaacgcc aagcacgaga ccgtcaacgt gcccggtact     2340

ggttccctct ggccgctgag cgcccccagc ccgatcggca agtgtgccca ccacagccac     2400

cacttctcct tggacatcga tgtgggctgc accgacctga acgaggactt tcggtag        2457


