                         SEQUENCE LISTING

<110>  The Brigham and Women's Hospital, Inc.
 
<120>  ESTROGEN SENSING THROUGH GPER1 REGULATES NORMAL AND MALIGNANT 
       LIVER GROWTH

<130>  29618-0180WO1

<140>  PCT/US2017/061632
<141>  2017-11-14

<150>  US 62/421,627
<151>  2016-11-14

<160>  20    

<170>  PatentIn version 3.5

<210>  1
<211>  60
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  genomic fragments

<400>  1
gtgattcaga tttatgtgaa tggcaccgag cagttcaatg cttcgtttga cttcaacata       60


<210>  2
<211>  31
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  genomic fragments

<400>  2
gcagttcaat gcttcgtttg acttcaacat a                                      31


<210>  3
<211>  375
<212>  PRT
<213>  Homo sapiens

<400>  3

Met Asp Val Thr Ser Gln Ala Arg Gly Val Gly Leu Glu Met Tyr Pro 
1               5                   10                  15      


Gly Thr Ala Gln Pro Ala Ala Pro Asn Thr Thr Ser Pro Glu Leu Asn 
            20                  25                  30          


Leu Ser His Pro Leu Leu Gly Thr Ala Leu Ala Asn Gly Thr Gly Glu 
        35                  40                  45              


Leu Ser Glu His Gln Gln Tyr Val Ile Gly Leu Phe Leu Ser Cys Leu 
    50                  55                  60                  


Tyr Thr Ile Phe Leu Phe Pro Ile Gly Phe Val Gly Asn Ile Leu Ile 
65                  70                  75                  80  


Leu Val Val Asn Ile Ser Phe Arg Glu Lys Met Thr Ile Pro Asp Leu 
                85                  90                  95      


Tyr Phe Ile Asn Leu Ala Val Ala Asp Leu Ile Leu Val Ala Asp Ser 
            100                 105                 110         


Leu Ile Glu Val Phe Asn Leu His Glu Arg Tyr Tyr Asp Ile Ala Val 
        115                 120                 125             


Leu Cys Thr Phe Met Ser Leu Phe Leu Gln Val Asn Met Tyr Ser Ser 
    130                 135                 140                 


Val Phe Phe Leu Thr Trp Met Ser Phe Asp Arg Tyr Ile Ala Leu Ala 
145                 150                 155                 160 


Arg Ala Met Arg Cys Ser Leu Phe Arg Thr Lys His His Ala Arg Leu 
                165                 170                 175     


Ser Cys Gly Leu Ile Trp Met Ala Ser Val Ser Ala Thr Leu Val Pro 
            180                 185                 190         


Phe Thr Ala Val His Leu Gln His Thr Asp Glu Ala Cys Phe Cys Phe 
        195                 200                 205             


Ala Asp Val Arg Glu Val Gln Trp Leu Glu Val Thr Leu Gly Phe Ile 
    210                 215                 220                 


Val Pro Phe Ala Ile Ile Gly Leu Cys Tyr Ser Leu Ile Val Arg Val 
225                 230                 235                 240 


Leu Val Arg Ala His Arg His Arg Gly Leu Arg Pro Arg Arg Gln Lys 
                245                 250                 255     


Ala Leu Arg Met Ile Leu Ala Val Val Leu Val Phe Phe Val Cys Trp 
            260                 265                 270         


Leu Pro Glu Asn Val Phe Ile Ser Val His Leu Leu Gln Arg Thr Gln 
        275                 280                 285             


Pro Gly Ala Ala Pro Cys Lys Gln Ser Phe Arg His Ala His Pro Leu 
    290                 295                 300                 


Thr Gly His Ile Val Asn Leu Ala Ala Phe Ser Asn Ser Cys Leu Asn 
305                 310                 315                 320 


Pro Leu Ile Tyr Ser Phe Leu Gly Glu Thr Phe Arg Asp Lys Leu Arg 
                325                 330                 335     


Leu Tyr Ile Glu Gln Lys Thr Asn Leu Pro Ala Leu Asn Arg Phe Cys 
            340                 345                 350         


His Ala Ala Leu Lys Ala Val Ile Pro Asp Ser Thr Glu Gln Ser Asp 
        355                 360                 365             


Val Arg Phe Ser Ser Ala Val 
    370                 375 


<210>  4
<211>  1128
<212>  DNA
<213>  Homo sapiens

<400>  4
atggatgtga cttcccaagc ccggggcgtg ggcctggaga tgtacccagg caccgcgcag       60

cctgcggccc ccaacaccac ctcccccgag ctcaacctgt cccacccgct cctgggcacc      120

gccctggcca atgggacagg tgagctctcg gagcaccagc agtacgtgat cggcctgttc      180

ctctcgtgcc tctacaccat cttcctcttc cccatcggct ttgtgggcaa catcctgatc      240

ctggtggtga acatcagctt ccgcgagaag atgaccatcc ccgacctgta cttcatcaac      300

ctggcggtgg cggacctcat cctggtggcc gactccctca ttgaggtgtt caacctgcac      360

gagcggtact acgacatcgc cgtcctgtgc accttcatgt cgctcttcct gcaggtcaac      420

atgtacagca gcgtcttctt cctcacctgg atgagcttcg accgctacat cgccctggcc      480

agggccatgc gctgcagcct gttccgcacc aagcaccacg cccggctgag ctgtggcctc      540

atctggatgg catccgtgtc agccacgctg gtgcccttca ccgccgtgca cctgcagcac      600

accgacgagg cctgcttctg tttcgcggat gtccgggagg tgcagtggct cgaggtcacg      660

ctgggcttca tcgtgccctt cgccatcatc ggcctgtgct actccctcat tgtccgggtg      720

ctggtcaggg cgcaccggca ccgtgggctg cggccccggc ggcagaaggc gctccgcatg      780

atcctcgcgg tggtgctggt cttcttcgtc tgctggctgc cggagaacgt cttcatcagc      840

gtgcacctcc tgcagcggac gcagcctggg gccgctccct gcaagcagtc tttccgccat      900

gcccaccccc tcacgggcca cattgtcaac ctcgccgcct tctccaacag ctgcctaaac      960

cccctcatct acagctttct cggggagacc ttcagggaca agctgaggct gtacattgag     1020

cagaaaacaa atttgccggc cctgaaccgc ttctgtcacg ctgccctgaa ggccgtcatt     1080

ccagacagca ccgagcagtc ggatgtgagg ttcagcagtg ccgtgtag                  1128


<210>  5
<211>  25
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Morpholino oligonucleotide targeting esr1

<400>  5
aggaaggttc ctccagggct tctct                                             25


<210>  6
<211>  25
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Morpholino oligonucleotide targeting esr2a

<400>  6
acatggtgaa ggcggatgag ttcag                                             25


<210>  7
<211>  25
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Morpholino oligonucleotide targeting

<400>  7
agctcatgct ggagaacaca agaga                                             25


<210>  8
<211>  25
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Morpholino oligonucleotide targeting gper1

<400>  8
acattggtag tctgctcctc catgc                                             25


<210>  9
<211>  25
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Morpholino oligonucleotide targeting gper1

<400>  9
gctgcaacac ctgttataag agaaa                                             25


<210>  10
<211>  25
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Morpholino oligonucleotide targeting mtor

<400>  10
ggtttgacac attaccctga gcatg                                             25


<210>  11
<211>  25
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Morpholino oligonucleotide targeting control

<400>  11
cctcttacct cagttacaat ttata                                             25


<210>  12
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PCR primer gper1 forward

<400>  12
tcaagttgcc gtcacaatgc                                                   20


<210>  13
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PCR primer gper1 reverse

<400>  13
gtcatcctct ccctgtggtt                                                   20


<210>  14
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PCR primer efl-alpha forward

<400>  14
gcgtcatcaa gagcgttgag                                                   20


<210>  15
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PCR primer efl-alpha reverse

<400>  15
ttggaacggt gtgattgagg                                                   20


<210>  16
<211>  27
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PCR primer mtor forward

<400>  16
ataagaaaag aaaccacatg tcatacc                                           27


<210>  17
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PCR primer mtor reverse

<400>  17
cttaccactc agagagacca aag                                               23


<210>  18
<211>  25
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PCR primer mtor LTR

<400>  18
ccctaagtac ttgtactttc acttg                                             25


<210>  19
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PCR primer gper1 forward

<400>  19
ctcgtgaata aagtgttgca g                                                 21


<210>  20
<211>  19
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PCR primer gper1 reverse

<400>  20
gcagtcttgt ttcctccag                                                    19


