                         SEQUENCE LISTING

<110>  North Carolina State University
       Hammerberg, Bruce
       Eguiluz-Hernandez, Sitka
 
<120>  TREATMENT OF ALLERGIC DISEASES WITH CHIMERIC PROTEIN

<130>  5051-905WO

<150>  US 62/511,535
<151>  2017-05-26

<150>  US 62,419,788
<151>  2016-11-09

<160>  17    

<170>  PatentIn version 3.5

<210>  1
<211>  18
<212>  PRT
<213>  Artificial

<220>
<223>  IgE epitope sequence

<400>  1

Val Asp Gly Gln Lys Ala Thr Asn Ile Phe Pro Tyr Thr Ala Pro Gly 
1               5                   10                  15      


Thr Lys 
        


<210>  2
<211>  10
<212>  PRT
<213>  Artificial

<220>
<223>  light chain complementarity determining region sequence

<400>  2

Arg Ala Ser Gly Asn Ile His Asn Tyr Leu 
1               5                   10  


<210>  3
<211>  7
<212>  PRT
<213>  Artificial

<220>
<223>  light chain complementarity determining region sequence

<400>  3

Asn Ala Lys Thr Leu Ala Asp 
1               5           


<210>  4
<211>  7
<212>  PRT
<213>  Artificial

<220>
<223>  light chain complementarity determining region sequence

<400>  4

Phe Trp Ser Thr Pro Tyr Thr 
1               5           


<210>  5
<211>  5
<212>  PRT
<213>  Artificial

<220>
<223>  heavy chain complementarity determining region sequence

<400>  5

Gly Tyr Thr Ile His 
1               5   


<210>  6
<211>  20
<212>  PRT
<213>  Artificial

<220>
<223>  heavy chain complementarity determining region sequence

<400>  6

Leu Ile Asn Pro Tyr Thr Gly Gly Ile Thr Tyr Asn Gln Asn Phe Lys 
1               5                   10                  15      


Gly Lys Ala Thr 
            20  


<210>  7
<211>  11
<212>  PRT
<213>  Artificial

<220>
<223>  heavy chain complementarity determining region sequence

<400>  7

Gly Pro Tyr Gly Asn Phe Tyr Ala Met Asp Tyr 
1               5                   10      


<210>  8
<211>  244
<212>  PRT
<213>  Artificial

<220>
<223>  single chain variable fragment sequence

<400>  8

Glu Val Gln Leu Gln Gln Ser Gly Pro Glu Leu Val Lys Pro Gly Ala 
1               5                   10                  15      


Ser Met Lys Ile Ser Cys Lys Ala Ser Gly Tyr Ser Ile Thr Gly Tyr 
            20                  25                  30          


Thr Ile His Trp Val Lys Gln Ser His Gly Lys Asn Leu Glu Trp Ile 
        35                  40                  45              


Gly Leu Ile Asn Pro Tyr Thr Gly Gly Ile Thr Tyr Asn Gln Asn Phe 
    50                  55                  60                  


Lys Gly Lys Ala Thr Leu Thr Val Asp Lys Ser Ser Ser Thr Ala Tyr 
65                  70                  75                  80  


Met Glu Leu Leu Ser Leu Thr Ser Glu Asp Ser Ala Val Tyr Tyr Cys 
                85                  90                  95      


Ser Arg Gly Pro Tyr Gly Asn Phe Tyr Ala Met Asp Tyr Trp Gly Gln 
            100                 105                 110         


Gly Thr Ser Val Thr Val Ser Ser Gly Gly Gly Gly Ser Gly Gly Gly 
        115                 120                 125             


Gly Ser Gly Gly Gly Gly Ser Asp Ile Gln Met Thr Gln Ser Pro Ala 
    130                 135                 140                 


Ser Leu Ser Ala Ser Val Gly Glu Thr Val Thr Ile Thr Cys Arg Ala 
145                 150                 155                 160 


Ser Gly Asn Ile His Asn Tyr Leu Ala Trp Tyr Gln Gln Lys Gln Gly 
                165                 170                 175     


Lys Ser Pro Gln Leu Leu Val Tyr Asn Ala Lys Thr Leu Ala Asp Ser 
            180                 185                 190         


Val Pro Ser Arg Phe Ser Gly Ser Gly Ser Gly Thr Gln Phe Ser Leu 
        195                 200                 205             


Lys Ile Asn Ser Leu Gln Pro Glu Asp Phe Gly Ser Tyr Tyr Cys Gln 
    210                 215                 220                 


His Phe Trp Ser Thr Pro Tyr Thr Phe Gly Gly Gly Thr Lys Leu Glu 
225                 230                 235                 240 


Ile Lys Arg Ala 
                


<210>  9
<211>  15
<212>  PRT
<213>  Artificial

<220>
<223>  linker peptide sequence

<400>  9

Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser 
1               5                   10                  15  


<210>  10
<211>  180
<212>  PRT
<213>  Homo sapiens

<400>  10

Val Pro Gln Lys Pro Lys Val Ser Leu Asn Pro Pro Trp Asn Arg Ile 
1               5                   10                  15      


Phe Lys Gly Glu Asn Val Thr Leu Thr Cys Asn Gly Asn Asn Phe Phe 
            20                  25                  30          


Glu Val Ser Ser Thr Lys Trp Phe His Asn Gly Ser Leu Ser Glu Glu 
        35                  40                  45              


Thr Asn Ser Ser Leu Asn Ile Val Asn Ala Lys Phe Glu Asp Ser Gly 
    50                  55                  60                  


Glu Tyr Lys Cys Gln His Gln Gln Val Asn Glu Ser Glu Pro Val Tyr 
65                  70                  75                  80  


Leu Glu Val Phe Ser Asp Trp Leu Leu Leu Gln Ala Ser Ala Glu Val 
                85                  90                  95      


Val Met Glu Gly Gln Pro Leu Phe Leu Arg Cys His Gly Trp Arg Asn 
            100                 105                 110         


Trp Asp Val Tyr Lys Val Ile Tyr Tyr Lys Asp Gly Glu Ala Leu Lys 
        115                 120                 125             


Tyr Trp Tyr Glu Asn His Asn Ile Ser Ile Thr Asn Ala Thr Val Glu 
    130                 135                 140                 


Asp Ser Gly Thr Tyr Tyr Cys Thr Gly Lys Val Trp Gln Leu Asp Tyr 
145                 150                 155                 160 


Glu Ser Glu Pro Leu Asn Ile Thr Val Ile Lys Ala Pro Arg Glu Lys 
                165                 170                 175     


Tyr Trp Leu Gln 
            180 


<210>  11
<211>  183
<212>  PRT
<213>  Canis lupus familiaris

<400>  11

Asp Thr Leu Lys Pro Thr Val Ser Met Asn Pro Pro Trp Asn Thr Ile 
1               5                   10                  15      


Leu Lys Asp Asp Ser Val Thr Leu Thr Cys Thr Gly Asn Asn Ser Leu 
            20                  25                  30          


Glu Val Asp Ser Ala Val Trp Leu His Asn Asn Thr Thr Leu Gln Glu 
        35                  40                  45              


Thr Thr Ser Arg Leu Asp Ile Asn Lys Ala Gln Ile Gln Asp Ser Gly 
    50                  55                  60                  


Glu Tyr Arg Cys Arg Glu Asn Arg Ser Ile Leu Ser Asp Pro Val Tyr 
65                  70                  75                  80  


Leu Thr Val Phe Thr Glu Trp Leu Ile Leu Gln Ala Ser Ala Asn Val 
                85                  90                  95      


Val Met Glu Gly Glu Ser Phe Leu Ile Arg Cys His Ser Trp Lys Asn 
            100                 105                 110         


Leu Arg Leu Thr Lys Val Thr Tyr Tyr Lys Asp Gly Ile Pro Ile Arg 
        115                 120                 125             


Tyr Trp Tyr Glu Asn Phe Asn Ile Ser Ile Ser Asn Val Thr Thr Lys 
    130                 135                 140                 


Asn Ser Gly Asn Tyr Ser Cys Ser Gly Gln Ile Gln Gln Lys Gly Tyr 
145                 150                 155                 160 


Thr Ser Lys Val Leu Asn Ile Ile Val Lys Lys Glu Pro Thr Lys Gln 
                165                 170                 175     


Asn Lys Tyr Ser Gly Leu Gln 
            180             


<210>  12
<211>  185
<212>  PRT
<213>  Felis catus

<400>  12

Gly Thr Arg Glu Pro Thr Val Ser Leu Asn Pro Pro Trp Thr Thr Ile 
1               5                   10                  15      


Leu Lys Glu Asp Ser Val Thr Leu Thr Cys Lys Glu Asn Asn Ser Leu 
            20                  25                  30          


Glu Leu Asn Ser Thr Val Trp Phe His Asn Lys Thr Lys Leu Gly Val 
        35                  40                  45              


Thr Thr Leu Thr Leu Asp Ile Val Lys Ala Gln Ile Arg Asp Ser Gly 
    50                  55                  60                  


Glu Tyr Thr Cys Gln Asn Lys Gly Ser Met Leu Ser Lys Pro Val Ser 
65                  70                  75                  80  


Leu Lys Val Phe Arg Glu Trp Leu Leu Leu Gln Ala Ser Thr Glu Val 
                85                  90                  95      


Val Leu Glu Gly Glu Ser Phe Leu Ile Arg Cys His Ser Trp Arg Asn 
            100                 105                 110         


Leu Asn Val Lys Lys Val Thr Tyr Tyr Arg Asn Gly Lys Phe Leu Gln 
        115                 120                 125             


Phe Trp Tyr Asp Asn Tyr Asn Ile Thr Ile Asn Asn Ala Thr Glu Thr 
    130                 135                 140                 


Asp Ser Gly Thr Tyr Tyr Cys Thr Gly Trp Ile Ser Lys Gln Asn His 
145                 150                 155                 160 


Ile Ser Asn Phe Leu Asn Ile Val Val Arg Lys Asp Ser Pro Pro Glu 
                165                 170                 175     


His Gln Ser Lys Tyr Tyr Trp Leu Gln 
            180                 185 


<210>  13
<211>  185
<212>  PRT
<213>  Equus caballus

<400>  13

Ala Ile Arg Lys Ser Thr Val Ser Leu Asn Pro Pro Trp Asn Arg Ile 
1               5                   10                  15      


Phe Arg Gly Glu Asn Val Thr Leu Thr Cys Asn Lys Asn Lys Pro Leu 
            20                  25                  30          


Lys Gly Asn Ser Thr Glu Trp Thr Tyr Asn Asn Thr Thr Leu Glu Val 
        35                  40                  45              


Thr Thr Ser Ser Leu Asn Ile Thr Asn Ala Ser His Arg Ser Ser Gly 
    50                  55                  60                  


Glu Tyr Arg Cys Arg Asn Asn Asp Leu Asn Leu Ser Glu Ala Val His 
65                  70                  75                  80  


Leu Glu Val Phe Ser Asp Trp Leu Leu Leu Gln Ala Ser Ala Glu Glu 
                85                  90                  95      


Val Ile Glu Gly Lys Ala Leu Val Leu Arg Cys Arg Gly Trp Lys Asp 
            100                 105                 110         


Trp Asp Val Phe Lys Val Ile Tyr Tyr Lys Asp Gly Lys Pro Leu Glu 
        115                 120                 125             


Tyr Trp Tyr Glu Asn Lys Asn Ile Ser Ile Glu Ser Ala Thr Thr Glu 
    130                 135                 140                 


Asn Ser Gly Thr Tyr Tyr Cys Glu Gly Ala Phe Asn Phe Lys Arg Thr 
145                 150                 155                 160 


Ser Glu Arg Tyr Thr Ser Asp Tyr Leu Asn Ile Thr Val Lys Lys Ala 
                165                 170                 175     


Glu Gln Ser Lys Arg Tyr Trp Leu Gln 
            180                 185 


<210>  14
<211>  442
<212>  PRT
<213>  Artificial

<220>
<223>  chimeric protein sequence

<400>  14

Asp Thr Leu Lys Pro Thr Val Ser Met Asn Pro Pro Trp Asn Thr Ile 
1               5                   10                  15      


Leu Lys Asp Asp Ser Val Thr Leu Thr Cys Thr Gly Asn Asn Ser Leu 
            20                  25                  30          


Glu Val Asp Ser Ala Val Trp Leu His Asn Asn Thr Thr Leu Gln Glu 
        35                  40                  45              


Thr Thr Ser Arg Leu Asp Ile Asn Lys Ala Gln Ile Gln Asp Ser Gly 
    50                  55                  60                  


Glu Tyr Arg Cys Arg Glu Asn Arg Ser Ile Leu Ser Asp Pro Val Tyr 
65                  70                  75                  80  


Leu Thr Val Phe Thr Glu Trp Leu Ile Leu Gln Ala Ser Ala Asn Val 
                85                  90                  95      


Val Met Glu Gly Glu Ser Phe Leu Ile Arg Cys His Ser Trp Lys Asn 
            100                 105                 110         


Leu Arg Leu Thr Lys Val Thr Tyr Tyr Lys Asp Gly Ile Pro Ile Arg 
        115                 120                 125             


Tyr Trp Tyr Glu Asn Phe Asn Ile Ser Ile Ser Asn Val Thr Thr Lys 
    130                 135                 140                 


Asn Ser Gly Asn Tyr Ser Cys Ser Gly Gln Ile Gln Gln Lys Gly Tyr 
145                 150                 155                 160 


Thr Ser Lys Val Leu Asn Ile Ile Val Lys Lys Glu Pro Thr Lys Gln 
                165                 170                 175     


Asn Lys Tyr Ser Gly Leu Gln Gly Gly Gly Gly Ser Gly Gly Gly Gly 
            180                 185                 190         


Ser Gly Gly Gly Gly Ser Glu Val Gln Leu Gln Gln Ser Gly Pro Glu 
        195                 200                 205             


Leu Val Lys Pro Gly Ala Ser Met Lys Ile Ser Cys Lys Ala Ser Gly 
    210                 215                 220                 


Tyr Ser Ile Thr Gly Tyr Thr Ile His Trp Val Lys Gln Ser His Gly 
225                 230                 235                 240 


Lys Asn Leu Glu Trp Ile Gly Leu Ile Asn Pro Tyr Thr Gly Gly Ile 
                245                 250                 255     


Thr Tyr Asn Gln Asn Phe Lys Gly Lys Ala Thr Leu Thr Val Asp Lys 
            260                 265                 270         


Ser Ser Ser Thr Ala Tyr Met Glu Leu Leu Ser Leu Thr Ser Glu Asp 
        275                 280                 285             


Ser Ala Val Tyr Tyr Cys Ser Arg Gly Pro Tyr Gly Asn Phe Tyr Ala 
    290                 295                 300                 


Met Asp Tyr Trp Gly Gln Gly Thr Ser Val Thr Val Ser Ser Gly Gly 
305                 310                 315                 320 


Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Asp Ile Gln 
                325                 330                 335     


Met Thr Gln Ser Pro Ala Ser Leu Ser Ala Ser Val Gly Glu Thr Val 
            340                 345                 350         


Thr Ile Thr Cys Arg Ala Ser Gly Asn Ile His Asn Tyr Leu Ala Trp 
        355                 360                 365             


Tyr Gln Gln Lys Gln Gly Lys Ser Pro Gln Leu Leu Val Tyr Asn Ala 
    370                 375                 380                 


Lys Thr Leu Ala Asp Ser Val Pro Ser Arg Phe Ser Gly Ser Gly Ser 
385                 390                 395                 400 


Gly Thr Gln Phe Ser Leu Lys Ile Asn Ser Leu Gln Pro Glu Asp Phe 
                405                 410                 415     


Gly Ser Tyr Tyr Cys Gln His Phe Trp Ser Thr Pro Tyr Thr Phe Gly 
            420                 425                 430         


Gly Gly Thr Lys Leu Glu Ile Lys Arg Ala 
        435                 440         


<210>  15
<211>  17
<212>  PRT
<213>  Artificial

<220>
<223>  IgE epitope sequence

<400>  15

Glu Asp Gly Gln Val Met Asp Val Asp Leu Ser Thr Ala Ser Thr Thr 
1               5                   10                  15      


Gln 
    


<210>  16
<211>  18
<212>  PRT
<213>  Artificial

<220>
<223>  IgE epitope sequence

<400>  16

Ile Asp Gly Gln Lys Val Asp Glu Gln Phe Pro Thr Gln His Gly Val 
1               5                   10                  15      


Lys Gln 
        


<210>  17
<211>  18
<212>  PRT
<213>  Artificial

<220>
<223>  IgE epitope sequence

<400>  17

Val Asp Gly Gln Lys Ala Thr Asn Ile Phe Pro Tyr Thr Ala Pro Gly 
1               5                   10                  15      


Lys Gln 
        


