                         SEQUENCE LISTING

<110>  Qilu Puget Sound Biotherapeutics Corporation
 
<120>  Anti-PD1 and Anti-CTLA4 Antibodies

<130>  SB-002-WO

<150>  US 62/419,157
<151>  2016-11-08

<160>  208   

<170>  PatentIn version 3.5

<210>  1
<211>  354
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  DNA sequence encoding the VH domain of the anti-hCTLA4 antibody designated 1E1

<400>  1
caggtgcagc ttgtcgaaag cggtggtggg gtagttgaac cagggcgttc tttacgttta       60

tcttgtgcag cctccggttt caccttcagt tcatacggga tgcactgggt tagacaagct      120

ccaggaaaag gattagaatg ggtagctgtt atttggtaca accctagcga gaaggattac      180

gccgattcag ctaagggtag gtttaccatt agtagagaca atagtaaaaa cactctatat      240

ctacaaatga acagcttgcg tgccgaggat actgcagttt actactgcgc gagggctggt      300

cttctcggtt atttcgacta ctggggtcag gggacattgg taactgtttc aagc            354


<210>  2
<211>  118
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Amino acid sequence of the VH domain of the anti-hCTLA4 antibody designated 1E1

<400>  2

Gln Val Gln Leu Val Glu Ser Gly Gly Gly Val Val Glu Pro Gly Arg 
1               5                   10                  15      


Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Phe Thr Phe Ser Ser Tyr 
            20                  25                  30          


Gly Met His Trp Val Arg Gln Ala Pro Gly Lys Gly Leu Glu Trp Val 
        35                  40                  45              


Ala Val Ile Trp Tyr Asn Pro Ser Glu Lys Asp Tyr Ala Asp Ser Ala 
    50                  55                  60                  


Lys Gly Arg Phe Thr Ile Ser Arg Asp Asn Ser Lys Asn Thr Leu Tyr 
65                  70                  75                  80  


Leu Gln Met Asn Ser Leu Arg Ala Glu Asp Thr Ala Val Tyr Tyr Cys 
                85                  90                  95      


Ala Arg Ala Gly Leu Leu Gly Tyr Phe Asp Tyr Trp Gly Gln Gly Thr 
            100                 105                 110         


Leu Val Thr Val Ser Ser 
        115             


<210>  3
<211>  5
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Amino acid sequence of the CDR1 of the VH domain of the anti-hCTLA4 antibodies designated 1E1, 2F1, 3G1, 4H1, 5B2, 6E3, 7A4, 8B4, 9C4, 10D4, 11F4, and 12G4

<400>  3

Ser Tyr Gly Met His 
1               5   


<210>  4
<211>  17
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Amino acid sequence of the CDR2 of the VH domain of the anti-hCTLA4 antibody designated 1E1

<400>  4

Val Ile Trp Tyr Asn Pro Ser Glu Lys Asp Tyr Ala Asp Ser Ala Lys 
1               5                   10                  15      


Gly 
    


<210>  5
<211>  9
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Amino acid sequence of the CDR3 of the VH domain of the anti-hCTLA4 antibodies designated 1E1, 2F1, 5B2, 6E3, 7A4, 9C4, and 10D4

<400>  5

Ala Gly Leu Leu Gly Tyr Phe Asp Tyr 
1               5                   


<210>  6
<211>  321
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  DNA sequence encoding the VL domain of the anti-hCTLA4 antibody designated 1E1

<400>  6
gagattgttc tgacacagag tcctggtaca ttatctttgt cccctggtga aagggcaact       60

ctatcttgta gggcctctca atctattagc agctacttgg cttggtatca acaaaaacca      120

ggtcaagcgc cgagaccatt gatttatggt gtctcctcta gagcaacagg gataccagac      180

agatttagtg gaagcggttc aggtactgat ttcactctaa cgattagccg tttagaacct      240

gaagattttg cagtgtacta ttgtcaacag tacggcatga gcccctttac ctttggtcct      300

ggaactaaag tggatataaa g                                                321


<210>  7
<211>  107
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Amino acid sequence of the VL domain of the anti-hCTLA4 antibody designated 1E1

<400>  7

Glu Ile Val Leu Thr Gln Ser Pro Gly Thr Leu Ser Leu Ser Pro Gly 
1               5                   10                  15      


Glu Arg Ala Thr Leu Ser Cys Arg Ala Ser Gln Ser Ile Ser Ser Tyr 
            20                  25                  30          


Leu Ala Trp Tyr Gln Gln Lys Pro Gly Gln Ala Pro Arg Pro Leu Ile 
        35                  40                  45              


Tyr Gly Val Ser Ser Arg Ala Thr Gly Ile Pro Asp Arg Phe Ser Gly 
    50                  55                  60                  


Ser Gly Ser Gly Thr Asp Phe Thr Leu Thr Ile Ser Arg Leu Glu Pro 
65                  70                  75                  80  


Glu Asp Phe Ala Val Tyr Tyr Cys Gln Gln Tyr Gly Met Ser Pro Phe 
                85                  90                  95      


Thr Phe Gly Pro Gly Thr Lys Val Asp Ile Lys 
            100                 105         


<210>  8
<211>  11
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Amino acid sequence of the CDR1 of the VL domain of the anti-hCTLA4 antibody designated 1E1

<400>  8

Arg Ala Ser Gln Ser Ile Ser Ser Tyr Leu Ala 
1               5                   10      


<210>  9
<211>  7
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Amino acid sequence of the CDR2 of the VL domain of the anti-hCTLA4 antibodies designated 1E1, 2F1, 3G1, 4H1, 5B2, 6E3, 7A4, 8B4, 9C4, 10D4, 11F4, and 12G4

<400>  9

Gly Val Ser Ser Arg Ala Thr 
1               5           


<210>  10
<211>  9
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Amino acid sequence of the CDR3 of the VL domain of the anti-hCTLA4 antibodies designated 1E1 and 6E3

<400>  10

Gln Gln Tyr Gly Met Ser Pro Phe Thr 
1               5                   


<210>  11
<211>  354
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  DNA sequence encoding the VH domain of the anti-hCTLA4 antibody designated 2F1

<400>  11
caggtgcagc ttgtcgaaag cggtggtggg gtagttgaac cagggcgttc tttacgttta       60

tcttgtgcag cctccggttt caccttcagt tcatacggga tgcactgggt tagacaagct      120

ccaggaaaag gattagaatg ggtagctgtt atttggtacc accgtagcga gaaggattac      180

gccgattcag ctaagggtag gtttaccatt agtagagaca atagtaaaaa cactctatat      240

ctacaaatga acagcttgcg tgccgaggat actgcagttt actactgcgc gagggctggt      300

cttctcggtt atttcgacta ctggggtcag gggacattgg taactgtttc aagc            354


<210>  12
<211>  118
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Amino acid sequence of the VH domain of the anti-hCTLA4 antibody designated 2F1

<400>  12

Gln Val Gln Leu Val Glu Ser Gly Gly Gly Val Val Glu Pro Gly Arg 
1               5                   10                  15      


Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Phe Thr Phe Ser Ser Tyr 
            20                  25                  30          


Gly Met His Trp Val Arg Gln Ala Pro Gly Lys Gly Leu Glu Trp Val 
        35                  40                  45              


Ala Val Ile Trp Tyr His Arg Ser Glu Lys Asp Tyr Ala Asp Ser Ala 
    50                  55                  60                  


Lys Gly Arg Phe Thr Ile Ser Arg Asp Asn Ser Lys Asn Thr Leu Tyr 
65                  70                  75                  80  


Leu Gln Met Asn Ser Leu Arg Ala Glu Asp Thr Ala Val Tyr Tyr Cys 
                85                  90                  95      


Ala Arg Ala Gly Leu Leu Gly Tyr Phe Asp Tyr Trp Gly Gln Gly Thr 
            100                 105                 110         


Leu Val Thr Val Ser Ser 
        115             


<210>  13
<211>  17
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Amino acid sequence of the CDR2 of the VH domain of the anti-hCTLA4 antibody designated 2F1

<400>  13

Val Ile Trp Tyr His Arg Ser Glu Lys Asp Tyr Ala Asp Ser Ala Lys 
1               5                   10                  15      


Gly 
    


<210>  14
<211>  321
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  DNA sequence encoding the VL domain of the anti-hCTLA4 antibodies designated 2F1 and 3G1

<400>  14
gagattgttc tgacacagag tcctggtaca ttatctttgt cccctggtga aagggcaact       60

ctatcttgta gggcctctca atctattaac agctacttgg cttggtatca acaaaaacca      120

ggtcaagcgc cgagaccatt gatttatggt gtctcctcta gagcaacagg gataccagac      180

agatttagtg gaagcggttc aggtactgat ttcactctaa cgattagccg tttagaacct      240

gaagattttg cagtgtacta ttgtcaacag tacggcatgt acccctttac ctttggtcct      300

ggaactaaag tggatataaa g                                                321


<210>  15
<211>  107
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Amino acid sequence of the VL domain of the anti-hCTLA4 antibodies designated 2F1 and 3G1

<400>  15

Glu Ile Val Leu Thr Gln Ser Pro Gly Thr Leu Ser Leu Ser Pro Gly 
1               5                   10                  15      


Glu Arg Ala Thr Leu Ser Cys Arg Ala Ser Gln Ser Ile Asn Ser Tyr 
            20                  25                  30          


Leu Ala Trp Tyr Gln Gln Lys Pro Gly Gln Ala Pro Arg Pro Leu Ile 
        35                  40                  45              


Tyr Gly Val Ser Ser Arg Ala Thr Gly Ile Pro Asp Arg Phe Ser Gly 
    50                  55                  60                  


Ser Gly Ser Gly Thr Asp Phe Thr Leu Thr Ile Ser Arg Leu Glu Pro 
65                  70                  75                  80  


Glu Asp Phe Ala Val Tyr Tyr Cys Gln Gln Tyr Gly Met Tyr Pro Phe 
                85                  90                  95      


Thr Phe Gly Pro Gly Thr Lys Val Asp Ile Lys 
            100                 105         


<210>  16
<211>  11
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Amino acid sequence of the CDR1 of the VL domain of the anti-hCTLA4 antibodies designated 2F1, 3G1, and 11F4

<400>  16

Arg Ala Ser Gln Ser Ile Asn Ser Tyr Leu Ala 
1               5                   10      


<210>  17
<211>  9
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Amino acid sequence of the CDR3 of the VL domain of the anti-hCTLA4 antibodies designated 2F1, 3G1,4H1, 5B2, 7A4, and 8B4

<400>  17

Gln Gln Tyr Gly Met Tyr Pro Phe Thr 
1               5                   


<210>  18
<211>  354
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  DNA sequence encoding the VH domain of the anti-hCTLA4 antibody designated 3G1

<400>  18
caggtgcagc ttgtcgaaag cggtggtggg gtagttgaac cagggcgttc tttacgttta       60

tcttgtacag cctccggttt caccttcagt tcatacggga tgcactgggt tagacaagct      120

ccaggaaaag gattagaatg ggtagctgtt atttggtaca agagtagcga aaagtattac      180

gccgattcag ctaagggtag gtttaccatt agtagagaca atagtaaaaa cactctatat      240

ctacaaatga acagcttgcg tgccgaggat actgcagttt actactgcgc gaggggtggt      300

cttctcggtt atttcgacta ctggggtcag gggacattgg taactgtttc aagc            354


<210>  19
<211>  118
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Amino acid sequence of the VH domain of the anti-hCTLA4 antibody designated 3G1

<400>  19

Gln Val Gln Leu Val Glu Ser Gly Gly Gly Val Val Glu Pro Gly Arg 
1               5                   10                  15      


Ser Leu Arg Leu Ser Cys Thr Ala Ser Gly Phe Thr Phe Ser Ser Tyr 
            20                  25                  30          


Gly Met His Trp Val Arg Gln Ala Pro Gly Lys Gly Leu Glu Trp Val 
        35                  40                  45              


Ala Val Ile Trp Tyr Lys Ser Ser Glu Lys Tyr Tyr Ala Asp Ser Ala 
    50                  55                  60                  


Lys Gly Arg Phe Thr Ile Ser Arg Asp Asn Ser Lys Asn Thr Leu Tyr 
65                  70                  75                  80  


Leu Gln Met Asn Ser Leu Arg Ala Glu Asp Thr Ala Val Tyr Tyr Cys 
                85                  90                  95      


Ala Arg Gly Gly Leu Leu Gly Tyr Phe Asp Tyr Trp Gly Gln Gly Thr 
            100                 105                 110         


Leu Val Thr Val Ser Ser 
        115             


<210>  20
<211>  17
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Amino acid sequence of the CDR2 of the VH domain of the anti-hCTLA4 antibody designated 3G1

<400>  20

Val Ile Trp Tyr Lys Ser Ser Glu Lys Tyr Tyr Ala Asp Ser Ala Lys 
1               5                   10                  15      


Gly 
    


<210>  21
<211>  9
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Amino acid sequence of the CDR3 of the VH domain of the anti-hCTLA4 antibody designated 3G1, 11F4, and 12G4

<400>  21

Gly Gly Leu Leu Gly Tyr Phe Asp Tyr 
1               5                   


<210>  22
<211>  354
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  DNA sequence encoding the VH domain of the anti-hCTLA4 antibody designated 4H1

<400>  22
caggtgcagc ttgtcgaaag cggtggtggg gtagttgaac cagggcgttc tttacgttta       60

tcttgtacag cctccggttt caccttcagt tcatacggga tgcactgggt tagacaagct      120

ccaggaaaag gattagaatg ggtagctgtt atttggtaca accctagcaa aaaggattac      180

gccgattcag ctaagggtag gtttaccatt agtagagaca atagtaaaaa cactctatat      240

ctacaaatga acagcttgcg tgccgaggat actgcagttt actactgcgc gaggggtggt      300

cttttcggtt atttcgacta ctggggtcag gggacattgg taactgtttc aagc            354


<210>  23
<211>  118
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Amino acid sequence of the VH domain of the anti-hCTLA4 antibody designated 4H1

<400>  23

Gln Val Gln Leu Val Glu Ser Gly Gly Gly Val Val Glu Pro Gly Arg 
1               5                   10                  15      


Ser Leu Arg Leu Ser Cys Thr Ala Ser Gly Phe Thr Phe Ser Ser Tyr 
            20                  25                  30          


Gly Met His Trp Val Arg Gln Ala Pro Gly Lys Gly Leu Glu Trp Val 
        35                  40                  45              


Ala Val Ile Trp Tyr Asn Pro Ser Lys Lys Asp Tyr Ala Asp Ser Ala 
    50                  55                  60                  


Lys Gly Arg Phe Thr Ile Ser Arg Asp Asn Ser Lys Asn Thr Leu Tyr 
65                  70                  75                  80  


Leu Gln Met Asn Ser Leu Arg Ala Glu Asp Thr Ala Val Tyr Tyr Cys 
                85                  90                  95      


Ala Arg Gly Gly Leu Phe Gly Tyr Phe Asp Tyr Trp Gly Gln Gly Thr 
            100                 105                 110         


Leu Val Thr Val Ser Ser 
        115             


<210>  24
<211>  17
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Amino acid sequence of the CDR2 of the VH domain of the anti-hCTLA4 antibody designated 4H1

<400>  24

Val Ile Trp Tyr Asn Pro Ser Lys Lys Asp Tyr Ala Asp Ser Ala Lys 
1               5                   10                  15      


Gly 
    


<210>  25
<211>  9
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Amino acid sequence of the CDR3 of the VH domain of the anti-hCTLA4 antibody designated 4H1

<400>  25

Gly Gly Leu Phe Gly Tyr Phe Asp Tyr 
1               5                   


<210>  26
<211>  321
<212>  DNA
<213>  Artificial Sequence

<220>
<223> DNA sequence encoding the VL domain of the anti-hCTLA4 antibodies designated 4H1 and 7A4

<400>  26
gagattgttc tgacacagag tcctggtaca ttatctttgt cccctggtga aagggcaact       60

ctatcttgta gggcctctca atctgttagc agctacttgg cttggtatca acaaaaacca      120

ggtcaagcgc cgagaccatt gatttatggt gtctcctcta gagcaacagg gataccagac      180

agatttagtg gaagcggttc aggtactgat ttcactctaa cgattagccg tttagaacct      240

gaagattttg cagtgtacta ttgtcaacag tacggcatgt acccctttac ctttggtcct      300

ggaactaaag tggatataaa g                                                321


<210>  27
<211>  107
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Amino acid sequence of the VL domain of the anti-hCTLA4 antibodies designated 4H1 and 7A4

<400>  27

Glu Ile Val Leu Thr Gln Ser Pro Gly Thr Leu Ser Leu Ser Pro Gly 
1               5                   10                  15      


Glu Arg Ala Thr Leu Ser Cys Arg Ala Ser Gln Ser Val Ser Ser Tyr 
            20                  25                  30          


Leu Ala Trp Tyr Gln Gln Lys Pro Gly Gln Ala Pro Arg Pro Leu Ile 
        35                  40                  45              


Tyr Gly Val Ser Ser Arg Ala Thr Gly Ile Pro Asp Arg Phe Ser Gly 
    50                  55                  60                  


Ser Gly Ser Gly Thr Asp Phe Thr Leu Thr Ile Ser Arg Leu Glu Pro 
65                  70                  75                  80  


Glu Asp Phe Ala Val Tyr Tyr Cys Gln Gln Tyr Gly Met Tyr Pro Phe 
                85                  90                  95      


Thr Phe Gly Pro Gly Thr Lys Val Asp Ile Lys 
            100                 105         


<210>  28
<211>  11
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Amino acid sequence of the CDR1 of the VL domain of the anti-hCTLA4 antibodies designated 4H1 and 7A4

<400>  28

Arg Ala Ser Gln Ser Val Ser Ser Tyr Leu Ala 
1               5                   10      


<210>  29
<211>  354
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  DNA sequence encoding the VH domain of the anti-hCTLA4 antibody designated 5B2

<400>  29
caggtgcagc ttgtcgaaag cggtggtggg gtagttgaac cagggcgttc tttacgttta       60

tcttgtacag cctccggttt caccttcagt tcatacggga tgcactgggt tagacaagct      120

ccaggaaaag gattagaatg ggtagctgtt atttggtaca agagtagcga aaaggattac      180

gccgattcag ctaagggtag gtttaccatt agtagagaca atagtaaaaa cactctatat      240

ctacaaatga acagcttgcg tgccgaggat actgcagttt actactgcgc gagggctggt      300

cttctcggtt atttcgacta ctggggtcag gggacattgg taactgtttc aagc            354


<210>  30
<211>  118
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Amino acid sequence of the VH domain of the anti-hCTLA4 antibody designated 5B2

<400>  30

Gln Val Gln Leu Val Glu Ser Gly Gly Gly Val Val Glu Pro Gly Arg 
1               5                   10                  15      


Ser Leu Arg Leu Ser Cys Thr Ala Ser Gly Phe Thr Phe Ser Ser Tyr 
            20                  25                  30          


Gly Met His Trp Val Arg Gln Ala Pro Gly Lys Gly Leu Glu Trp Val 
        35                  40                  45              


Ala Val Ile Trp Tyr Lys Ser Ser Glu Lys Asp Tyr Ala Asp Ser Ala 
    50                  55                  60                  


Lys Gly Arg Phe Thr Ile Ser Arg Asp Asn Ser Lys Asn Thr Leu Tyr 
65                  70                  75                  80  


Leu Gln Met Asn Ser Leu Arg Ala Glu Asp Thr Ala Val Tyr Tyr Cys 
                85                  90                  95      


Ala Arg Ala Gly Leu Leu Gly Tyr Phe Asp Tyr Trp Gly Gln Gly Thr 
            100                 105                 110         


Leu Val Thr Val Ser Ser 
        115             


<210>  31
<211>  17
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Amino acid sequence of the CDR2 of the VH domain of the anti-hCTLA4 antibodies designated 5B2 and 9C4

<400>  31

Val Ile Trp Tyr Lys Ser Ser Glu Lys Asp Tyr Ala Asp Ser Ala Lys 
1               5                   10                  15      


Gly 
    


<210>  32
<211>  321
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  DNA sequence encoding the VL domain of the anti-hCTLA4 antibody designated 5B2

<400>  32
gagattgttc tgacacagag tcctggtaca ttatctttgt cccctggtga aagggcaact       60

ctatcttgta gggcctctca atctcttagc agctacttgg cttggtatca acaaaaacca      120

ggtcaagcgc cgagactatt gatttatggt gtctcctcta gagcaacagg gataccagac      180

agatttagtg gaagcggttc aggtactgat ttcactctaa cgattagtcg tttagaacct      240

gaagattttg cagtgtacta ttgtcaacag tacggcatgt acccctttac ctttggtcct      300

ggaactaaag tggatataaa g                                                321


<210>  33
<211>  107
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Amino acid sequence of the VL domain of the anti-hCTLA4 antibody designated 5B2

<400>  33

Glu Ile Val Leu Thr Gln Ser Pro Gly Thr Leu Ser Leu Ser Pro Gly 
1               5                   10                  15      


Glu Arg Ala Thr Leu Ser Cys Arg Ala Ser Gln Ser Leu Ser Ser Tyr 
            20                  25                  30          


Leu Ala Trp Tyr Gln Gln Lys Pro Gly Gln Ala Pro Arg Leu Leu Ile 
        35                  40                  45              


Tyr Gly Val Ser Ser Arg Ala Thr Gly Ile Pro Asp Arg Phe Ser Gly 
    50                  55                  60                  


Ser Gly Ser Gly Thr Asp Phe Thr Leu Thr Ile Ser Arg Leu Glu Pro 
65                  70                  75                  80  


Glu Asp Phe Ala Val Tyr Tyr Cys Gln Gln Tyr Gly Met Tyr Pro Phe 
                85                  90                  95      


Thr Phe Gly Pro Gly Thr Lys Val Asp Ile Lys 
            100                 105         


<210>  34
<211>  11
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Amino acid sequence of the CDR1 of the VL domain of the anti-hCTLA4 antibody designated 5B2

<400>  34

Arg Ala Ser Gln Ser Leu Ser Ser Tyr Leu Ala 
1               5                   10      


<210>  35
<211>  354
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  DNA sequence encoding the VH domain of the anti-hCTLA4 antibody designated 6E3

<400>  35
caggtgcagc ttgtcgaaag cggtggtggg gtagttgaac cagggcgttc tttacgttta       60

tcttgtgcag cctccggttt caccttcagt tcatacggga tgcactgggt tagacaagct      120

ccaggaaaag gattagaatg ggtagctgtt atttggtaca agactagcga gaaggattac      180

gccgattcag ctaagggtag gtttaccatt agtagagaca atagtaaaaa cactctatat      240

ctacaaatga acagcttgcg tgccgaggat actgcagttt actactgcgc gagggctggt      300

cttctcggtt atttcgacta ctggggtcag gggacattgg taactgtttc aagc            354


<210>  36
<211>  118
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Amino acid sequence of the VH domain of the anti-hCTLA4 antibody designated 6E3

<400>  36

Gln Val Gln Leu Val Glu Ser Gly Gly Gly Val Val Glu Pro Gly Arg 
1               5                   10                  15      


Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Phe Thr Phe Ser Ser Tyr 
            20                  25                  30          


Gly Met His Trp Val Arg Gln Ala Pro Gly Lys Gly Leu Glu Trp Val 
        35                  40                  45              


Ala Val Ile Trp Tyr Lys Thr Ser Glu Lys Asp Tyr Ala Asp Ser Ala 
    50                  55                  60                  


Lys Gly Arg Phe Thr Ile Ser Arg Asp Asn Ser Lys Asn Thr Leu Tyr 
65                  70                  75                  80  


Leu Gln Met Asn Ser Leu Arg Ala Glu Asp Thr Ala Val Tyr Tyr Cys 
                85                  90                  95      


Ala Arg Ala Gly Leu Leu Gly Tyr Phe Asp Tyr Trp Gly Gln Gly Thr 
            100                 105                 110         


Leu Val Thr Val Ser Ser 
        115             


<210>  37
<211>  17
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Amino acid sequence of the CDR2 of the VH domain of the anti-hCTLA4 antibody designated 6E3

<400>  37

Val Ile Trp Tyr Lys Thr Ser Glu Lys Asp Tyr Ala Asp Ser Ala Lys 
1               5                   10                  15      


Gly 
    


<210>  38
<211>  321
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  DNA sequence encoding the VL domain of the anti-hCTLA4 antibody designated 6E3

<400>  38
gagattgttc tgacacagag tcctggtaca ttatctttgt cccctggtga aagggcaact       60

ctatcttgta gggcctctca atctgttacc agctacttgg cttggtatca acaaaaacca      120

ggtcaagcgc cgagaccatt gatttatggt gtctcctcta gagcaacagg gataccagac      180

agatttagtg gaagcggttc aggtactgat ttcactctaa cgattagccg tttagaacct      240

gaagattttg cagtgtacta ttgtcaacag tacggcatga gcccctttac ctttggtcct      300

ggaactaaag tggatataaa g                                                321


<210>  39
<211>  107
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Amino acid sequence of the VL domain of the anti-hCTLA4 antibody designated 6E3

<400>  39

Glu Ile Val Leu Thr Gln Ser Pro Gly Thr Leu Ser Leu Ser Pro Gly 
1               5                   10                  15      


Glu Arg Ala Thr Leu Ser Cys Arg Ala Ser Gln Ser Val Thr Ser Tyr 
            20                  25                  30          


Leu Ala Trp Tyr Gln Gln Lys Pro Gly Gln Ala Pro Arg Pro Leu Ile 
        35                  40                  45              


Tyr Gly Val Ser Ser Arg Ala Thr Gly Ile Pro Asp Arg Phe Ser Gly 
    50                  55                  60                  


Ser Gly Ser Gly Thr Asp Phe Thr Leu Thr Ile Ser Arg Leu Glu Pro 
65                  70                  75                  80  


Glu Asp Phe Ala Val Tyr Tyr Cys Gln Gln Tyr Gly Met Ser Pro Phe 
                85                  90                  95      


Thr Phe Gly Pro Gly Thr Lys Val Asp Ile Lys 
            100                 105         


<210>  40
<211>  11
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Amino acid sequence of the CDR1 of the VL domain of the anti-hCTLA4 antibodies designated 6E3 and 10D4

<400>  40

Arg Ala Ser Gln Ser Val Thr Ser Tyr Leu Ala 
1               5                   10      


<210>  41
<211>  354
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Nucleotide sequence encoding the VH domain of the anti-hCTLA4 antibody designated 7A4

<400>  41
caggtgcagc ttgtcgaaag cggtggtggg gtagttgaac cagggcgttc tttacgttta       60

tcttgtacag cctccggttt caccttcagt tcatacggga tgcactgggt tagacaagct      120

ccaggaaaag gattagaatg ggtagctgtt atttggtacc agactagcga aaaggattac      180

gccgattcag ctaagggtag gtttaccatt agtagagaca atagtaaaaa cactctatat      240

ctacaaatga acagcttgcg tgccgaggat actgcagttt actactgcgc gagggctggt      300

cttctcggtt atttcgacta ctggggtcag gggacattgg taactgtttc aagc            354


<210>  42
<211>  118
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Amino acid sequence of the VH domain of the anti-hCTLA4 antibody designated 7A4

<400>  42

Gln Val Gln Leu Val Glu Ser Gly Gly Gly Val Val Glu Pro Gly Arg 
1               5                   10                  15      


Ser Leu Arg Leu Ser Cys Thr Ala Ser Gly Phe Thr Phe Ser Ser Tyr 
            20                  25                  30          


Gly Met His Trp Val Arg Gln Ala Pro Gly Lys Gly Leu Glu Trp Val 
        35                  40                  45              


Ala Val Ile Trp Tyr Gln Thr Ser Glu Lys Asp Tyr Ala Asp Ser Ala 
    50                  55                  60                  


Lys Gly Arg Phe Thr Ile Ser Arg Asp Asn Ser Lys Asn Thr Leu Tyr 
65                  70                  75                  80  


Leu Gln Met Asn Ser Leu Arg Ala Glu Asp Thr Ala Val Tyr Tyr Cys 
                85                  90                  95      


Ala Arg Ala Gly Leu Leu Gly Tyr Phe Asp Tyr Trp Gly Gln Gly Thr 
            100                 105                 110         


Leu Val Thr Val Ser Ser 
        115             


<210>  43
<211>  17
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Amino acid sequence of the CDR2 of the VH domain of the anti-hCTLA4 antibody designated 7A4

<400>  43

Val Ile Trp Tyr Gln Thr Ser Glu Lys Asp Tyr Ala Asp Ser Ala Lys 
1               5                   10                  15      


Gly 
    


<210>  44
<211>  354
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  DNA sequence encoding the VH domain of the anti-hCTLA4 antibody designated 8B4

<400>  44
caggtgcagc ttgtcgaaag cggtggtggg gtagttgaac cagggcgttc tttacgttta       60

tcttgtgcag cctccggttt caccttcagt tcatacggga tgcactgggt tagacaagct      120

ccaggaaaag gattagaatg ggtagctgtt atttggtacc agcctagcga gaaggattac      180

gccgattcag ctaagggtag gtttaccatt agtagagaca atagtaaaaa cactctatat      240

ctacaaatga acagcttgcg tgccgaggat actgcagttt actactgcgc gagggctggt      300

tttctcggtt atttcgacta ctggggtcag gggacattgg taactgtttc aagc            354


<210>  45
<211>  118
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Amino acid sequence of the VH domain of the anti-hCTLA4 antibody designated 8B4

<400>  45

Gln Val Gln Leu Val Glu Ser Gly Gly Gly Val Val Glu Pro Gly Arg 
1               5                   10                  15      


Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Phe Thr Phe Ser Ser Tyr 
            20                  25                  30          


Gly Met His Trp Val Arg Gln Ala Pro Gly Lys Gly Leu Glu Trp Val 
        35                  40                  45              


Ala Val Ile Trp Tyr Gln Pro Ser Glu Lys Asp Tyr Ala Asp Ser Ala 
    50                  55                  60                  


Lys Gly Arg Phe Thr Ile Ser Arg Asp Asn Ser Lys Asn Thr Leu Tyr 
65                  70                  75                  80  


Leu Gln Met Asn Ser Leu Arg Ala Glu Asp Thr Ala Val Tyr Tyr Cys 
                85                  90                  95      


Ala Arg Ala Gly Phe Leu Gly Tyr Phe Asp Tyr Trp Gly Gln Gly Thr 
            100                 105                 110         


Leu Val Thr Val Ser Ser 
        115             


<210>  46
<211>  17
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Amino acid sequence of the CDR2 of the VH domain of the anti-hCTLA4 antibody designated 8B4

<400>  46

Val Ile Trp Tyr Gln Pro Ser Glu Lys Asp Tyr Ala Asp Ser Ala Lys 
1               5                   10                  15      


Gly 
    


<210>  47
<211>  9
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Amino acid sequence of the CDR3 of the VH domain of the anti-hCTLA4 antibody designated 8B4

<400>  47

Ala Gly Phe Leu Gly Tyr Phe Asp Tyr 
1               5                   


<210>  48
<211>  321
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  DNA sequence encoding the VL domain of the anti-hCTLA4 antibody designated 8B4

<400>  48
gagattgttc tgacacagag tcctggtaca ttatctttgt cccctggtga aagggcaact       60

ctatcttgta gggcctctca atctgttaac agctacttgg cttggtatca acaaaaacca      120

ggtcaagcgc cgagaccatt gatttatggt gtctcctcta gagcaacagg gataccagac      180

agatttagtg gaagcggttc aggtactgat ttcactctaa cgattagccg tttagaacct      240

gaagattttg cagtgtacta ttgtcaacag tacggcatgt acccctttac ctttggtcct      300

ggaactaaag tggatataaa g                                                321


<210>  49
<211>  107
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Amino acid sequence of the VL domain of the anti-hCTLA4 antibody designated 8B4

<400>  49

Glu Ile Val Leu Thr Gln Ser Pro Gly Thr Leu Ser Leu Ser Pro Gly 
1               5                   10                  15      


Glu Arg Ala Thr Leu Ser Cys Arg Ala Ser Gln Ser Val Asn Ser Tyr 
            20                  25                  30          


Leu Ala Trp Tyr Gln Gln Lys Pro Gly Gln Ala Pro Arg Pro Leu Ile 
        35                  40                  45              


Tyr Gly Val Ser Ser Arg Ala Thr Gly Ile Pro Asp Arg Phe Ser Gly 
    50                  55                  60                  


Ser Gly Ser Gly Thr Asp Phe Thr Leu Thr Ile Ser Arg Leu Glu Pro 
65                  70                  75                  80  


Glu Asp Phe Ala Val Tyr Tyr Cys Gln Gln Tyr Gly Met Tyr Pro Phe 
                85                  90                  95      


Thr Phe Gly Pro Gly Thr Lys Val Asp Ile Lys 
            100                 105         


<210>  50
<211>  11
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Amino acid sequence of the CDR1 of the VL domain of the anti-hCTLA4 antibody designated 8B4

<400>  50

Arg Ala Ser Gln Ser Val Asn Ser Tyr Leu Ala 
1               5                   10      


<210>  51
<211>  354
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  DNA sequence encoding the VH domain of the anti-hCTLA4 antibody designated 9C4

<400>  51
caggtgcagc ttgtcgaaag cggtggtggg gtagttgaac cagggcgttc tttacgttta       60

tcttgtgcag cctccggttt caccttcagt tcatacggga tgcactgggt tagacaagct      120

ccaggaaaag gattagaatg ggtagctgtt atttggtaca agagtagcga aaaggattac      180

gccgattcag ctaagggtag gtttaccatt agtagagaca atagtaaaaa cactctatat      240

ctacaaatga acagcttgcg tgccgaggat actgcagttt actactgcgc gagggctggt      300

cttctcggtt atttcgacta ctggggtcag gggacattgg taactgtttc aagc            354


<210>  52
<211>  118
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Amino acid sequence of the VH domain of the anti-hCTLA4 antibody designated 9C4

<400>  52

Gln Val Gln Leu Val Glu Ser Gly Gly Gly Val Val Glu Pro Gly Arg 
1               5                   10                  15      


Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Phe Thr Phe Ser Ser Tyr 
            20                  25                  30          


Gly Met His Trp Val Arg Gln Ala Pro Gly Lys Gly Leu Glu Trp Val 
        35                  40                  45              


Ala Val Ile Trp Tyr Lys Ser Ser Glu Lys Asp Tyr Ala Asp Ser Ala 
    50                  55                  60                  


Lys Gly Arg Phe Thr Ile Ser Arg Asp Asn Ser Lys Asn Thr Leu Tyr 
65                  70                  75                  80  


Leu Gln Met Asn Ser Leu Arg Ala Glu Asp Thr Ala Val Tyr Tyr Cys 
                85                  90                  95      


Ala Arg Ala Gly Leu Leu Gly Tyr Phe Asp Tyr Trp Gly Gln Gly Thr 
            100                 105                 110         


Leu Val Thr Val Ser Ser 
        115             


<210>  53
<211>  321
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  DNA sequence encoding the VL domain of the anti-hCTLA4 antibody designated 9C4

<400>  53
gagattgttc tgacacagag tcctggtaca ttatctttgt cccctggtga aagggcaact       60

ctatcttgta gggcctctca atctcttaac agctacttgg cttggtatca acaaaaacca      120

ggtcaagcgc cgagaccatt gatttatggt gtctcctcta gagcaacagg gataccagac      180

agatttagtg gaagcggttc aggtactgat ttcactctaa cgattagccg tttagaacct      240

gaagattttg cagtgtacta ttgtcaacag tacggcatat acccctttac ctttggtcct      300

ggaactaaag tggatataaa g                                                321


<210>  54
<211>  107
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Amino acid sequence of the VL domain of the anti-hCTLA4 antibody designated 9C4

<400>  54

Glu Ile Val Leu Thr Gln Ser Pro Gly Thr Leu Ser Leu Ser Pro Gly 
1               5                   10                  15      


Glu Arg Ala Thr Leu Ser Cys Arg Ala Ser Gln Ser Leu Asn Ser Tyr 
            20                  25                  30          


Leu Ala Trp Tyr Gln Gln Lys Pro Gly Gln Ala Pro Arg Pro Leu Ile 
        35                  40                  45              


Tyr Gly Val Ser Ser Arg Ala Thr Gly Ile Pro Asp Arg Phe Ser Gly 
    50                  55                  60                  


Ser Gly Ser Gly Thr Asp Phe Thr Leu Thr Ile Ser Arg Leu Glu Pro 
65                  70                  75                  80  


Glu Asp Phe Ala Val Tyr Tyr Cys Gln Gln Tyr Gly Ile Tyr Pro Phe 
                85                  90                  95      


Thr Phe Gly Pro Gly Thr Lys Val Asp Ile Lys 
            100                 105         


<210>  55
<211>  11
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Amino acid sequence of the CDR1 of the VL domain of the anti-hCTLA4 antibody designated 9C4

<400>  55

Arg Ala Ser Gln Ser Leu Asn Ser Tyr Leu Ala 
1               5                   10      


<210>  56
<211>  9
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Amino acid sequence of the CDR3 of the VL domain of the anti-hCTLA4 antibody designated 9C4

<400>  56

Gln Gln Tyr Gly Ile Tyr Pro Phe Thr 
1               5                   


<210>  57
<211>  354
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  DNA sequence encoding the VH domain of the anti-hCTLA4 antibody designated 10D4

<400>  57
caggtgcagc ttgtcgaaag cggtggtggg gtagttgaac cagggcgttc tttacgttta       60

tcttgtacag cctccggttt caccttcagt tcatacggga tgcactgggt tagacaagct      120

ccaggaaaag gattagaatg ggtagctgtt atttggtacc accctagcaa aaaggattac      180

gccgattcag ctaagggtag gtttaccatt agtagagaca atagtaaaaa cactctatat      240

ctacaaatga acagcttgcg tgccgaggat actgcagttt actactgcgc gagggctggt      300

cttctcggtt atttcgacta ctggggtcag gggacattgg taactgtttc aagc            354


<210>  58
<211>  118
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Amino acid sequence of the VH domain of the anti-hCTLA4 antibody designated 10D4

<400>  58

Gln Val Gln Leu Val Glu Ser Gly Gly Gly Val Val Glu Pro Gly Arg 
1               5                   10                  15      


Ser Leu Arg Leu Ser Cys Thr Ala Ser Gly Phe Thr Phe Ser Ser Tyr 
            20                  25                  30          


Gly Met His Trp Val Arg Gln Ala Pro Gly Lys Gly Leu Glu Trp Val 
        35                  40                  45              


Ala Val Ile Trp Tyr His Pro Ser Lys Lys Asp Tyr Ala Asp Ser Ala 
    50                  55                  60                  


Lys Gly Arg Phe Thr Ile Ser Arg Asp Asn Ser Lys Asn Thr Leu Tyr 
65                  70                  75                  80  


Leu Gln Met Asn Ser Leu Arg Ala Glu Asp Thr Ala Val Tyr Tyr Cys 
                85                  90                  95      


Ala Arg Ala Gly Leu Leu Gly Tyr Phe Asp Tyr Trp Gly Gln Gly Thr 
            100                 105                 110         


Leu Val Thr Val Ser Ser 
        115             


<210>  59
<211>  17
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Amino acid sequence of the CDR2 of the VH domain of the anti-hCTLA4 antibody designated 10D4

<400>  59

Val Ile Trp Tyr His Pro Ser Lys Lys Asp Tyr Ala Asp Ser Ala Lys 
1               5                   10                  15      


Gly 
    


<210>  60
<211>  321
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  DNA sequence encoding the VL domain of the anti-hCTLA4 antibody designated 10D4

<400>  60
gagattgttc tgacacagag tcctggtaca ttatctttgt cccctggtga aagggcaact       60

ctatcttgta gggcctctca atctgttacc agctacttgg cttggtatca acaaaaacca      120

ggtcaagcgc cgagaccatt gatttatggt gtctcctcta gagcaacagg gataccagac      180

agatttagtg gaagcggttc aggtactgat ttcactctaa cgattagccg tttagaacct      240

gaagattttg cagtgtacta ttgtcaacag tacggcagat acccctttac ctttggtcct      300

ggaactaaag tggatataaa g                                                321


<210>  61
<211>  107
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Amino acid sequence of the VL domain of the anti-hCTLA4 antibody designated 10D4

<400>  61

Glu Ile Val Leu Thr Gln Ser Pro Gly Thr Leu Ser Leu Ser Pro Gly 
1               5                   10                  15      


Glu Arg Ala Thr Leu Ser Cys Arg Ala Ser Gln Ser Val Thr Ser Tyr 
            20                  25                  30          


Leu Ala Trp Tyr Gln Gln Lys Pro Gly Gln Ala Pro Arg Pro Leu Ile 
        35                  40                  45              


Tyr Gly Val Ser Ser Arg Ala Thr Gly Ile Pro Asp Arg Phe Ser Gly 
    50                  55                  60                  


Ser Gly Ser Gly Thr Asp Phe Thr Leu Thr Ile Ser Arg Leu Glu Pro 
65                  70                  75                  80  


Glu Asp Phe Ala Val Tyr Tyr Cys Gln Gln Tyr Gly Arg Tyr Pro Phe 
                85                  90                  95      


Thr Phe Gly Pro Gly Thr Lys Val Asp Ile Lys 
            100                 105         


<210>  62
<211>  9
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Amino acid sequence of the CDR3 of the VL domain of the anti-hCTLA4 antibodies designated 10D4, 11F4, and 12G4

<400>  62

Gln Gln Tyr Gly Arg Tyr Pro Phe Thr 
1               5                   


<210>  63
<211>  354
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  DNA sequence encoding the VH domain of the anti-hCTLA4 antibody designated 11F4

<400>  63
caggtgcagc ttgtcgaaag cggtggtggg gtagttgaac cagggcgttc tttacgttta       60

tcttgtgcag cctccggttt caccttcagt tcatacggga tgcactgggt tagacaagct      120

ccaggaaaag gattagaatg ggtagctgtt atttggtaca agcctagcga gaaggattac      180

gccgattcag ctaagggtag gtttaccatt agtagagaca atagtaaaaa cactctatat      240

ctacaaatga acagcttgcg tgccgaggat actgcagttt actactgcgc gaggggtggt      300

cttctcggtt atttcgacta ctggggtcag gggacattgg taactgtttc aagc            354


<210>  64
<211>  118
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Amino acid sequence of the VH domain of the anti-hCTLA4 antibody designated 11F4

<400>  64

Gln Val Gln Leu Val Glu Ser Gly Gly Gly Val Val Glu Pro Gly Arg 
1               5                   10                  15      


Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Phe Thr Phe Ser Ser Tyr 
            20                  25                  30          


Gly Met His Trp Val Arg Gln Ala Pro Gly Lys Gly Leu Glu Trp Val 
        35                  40                  45              


Ala Val Ile Trp Tyr Lys Pro Ser Glu Lys Asp Tyr Ala Asp Ser Ala 
    50                  55                  60                  


Lys Gly Arg Phe Thr Ile Ser Arg Asp Asn Ser Lys Asn Thr Leu Tyr 
65                  70                  75                  80  


Leu Gln Met Asn Ser Leu Arg Ala Glu Asp Thr Ala Val Tyr Tyr Cys 
                85                  90                  95      


Ala Arg Gly Gly Leu Leu Gly Tyr Phe Asp Tyr Trp Gly Gln Gly Thr 
            100                 105                 110         


Leu Val Thr Val Ser Ser 
        115             


<210>  65
<211>  17
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Amino acid sequence of the CDR2 of the VH domain of the anti-hCTLA4 antibody designated 11F4

<400>  65

Val Ile Trp Tyr Lys Pro Ser Glu Lys Asp Tyr Ala Asp Ser Ala Lys 
1               5                   10                  15      


Gly 
    


<210>  66
<211>  321
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  DNA sequence encoding the VL domain of the anti-hCTLA4 antibody designated 11F4

<400>  66
gagattgttc tgacacagag tcctggtaca ttatctttgt cccctggtga aagggcaact       60

ctatcttgta gggcctctca atctattaac agctacttgg cttggtatca acaaaaacca      120

ggtcaagcgc cgagaccatt gatttatggt gtctcctcta gagcaacagg gataccagac      180

agatttagtg gaagcggttc aggtactgat ttcactctaa cgattagccg tttagaacct      240

gaagattttg cagtgtacta ttgtcaacag tacggcaggt acccctttac ctttggtcct      300

ggaactaaag tggatataaa g                                                321


<210>  67
<211>  107
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Amino acid sequence of the VL domain of the anti-hCTLA4 antibody designated 11F4

<400>  67

Glu Ile Val Leu Thr Gln Ser Pro Gly Thr Leu Ser Leu Ser Pro Gly 
1               5                   10                  15      


Glu Arg Ala Thr Leu Ser Cys Arg Ala Ser Gln Ser Ile Asn Ser Tyr 
            20                  25                  30          


Leu Ala Trp Tyr Gln Gln Lys Pro Gly Gln Ala Pro Arg Pro Leu Ile 
        35                  40                  45              


Tyr Gly Val Ser Ser Arg Ala Thr Gly Ile Pro Asp Arg Phe Ser Gly 
    50                  55                  60                  


Ser Gly Ser Gly Thr Asp Phe Thr Leu Thr Ile Ser Arg Leu Glu Pro 
65                  70                  75                  80  


Glu Asp Phe Ala Val Tyr Tyr Cys Gln Gln Tyr Gly Arg Tyr Pro Phe 
                85                  90                  95      


Thr Phe Gly Pro Gly Thr Lys Val Asp Ile Lys 
            100                 105         


<210>  68
<211>  354
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  DNA sequence encoding the VH domain of the anti-hCTLA4 antibody designated 12G4

<400>  68
caggtgcagc ttgtcgaaag cggtggtggg gtagttgaac cagggcgttc tttacgttta       60

tcttgtgcag cctctggttt caccttcagt tcatacggga tgcactgggt tagacaagct      120

ccaggaaaag gattagaatg ggtagctgtt atttggtaca acactagcaa gaaggattac      180

gccgattcag ctaagggtag gtttaccatt agtagagaca atagtaaaaa cactctatat      240

ctacaaatga acagcttgcg tgccgaggat actgcagttt actactgcgc gaggggtggt      300

cttctcggtt atttcgacta ctggggtcag gggacattgg taactgtttc aagc            354


<210>  69
<211>  118
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Amino acid sequence of the VH domain of the anti-hCTLA4 antibody designated 12G4

<400>  69

Gln Val Gln Leu Val Glu Ser Gly Gly Gly Val Val Glu Pro Gly Arg 
1               5                   10                  15      


Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Phe Thr Phe Ser Ser Tyr 
            20                  25                  30          


Gly Met His Trp Val Arg Gln Ala Pro Gly Lys Gly Leu Glu Trp Val 
        35                  40                  45              


Ala Val Ile Trp Tyr Asn Thr Ser Lys Lys Asp Tyr Ala Asp Ser Ala 
    50                  55                  60                  


Lys Gly Arg Phe Thr Ile Ser Arg Asp Asn Ser Lys Asn Thr Leu Tyr 
65                  70                  75                  80  


Leu Gln Met Asn Ser Leu Arg Ala Glu Asp Thr Ala Val Tyr Tyr Cys 
                85                  90                  95      


Ala Arg Gly Gly Leu Leu Gly Tyr Phe Asp Tyr Trp Gly Gln Gly Thr 
            100                 105                 110         


Leu Val Thr Val Ser Ser 
        115             


<210>  70
<211>  17
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Amino acid sequence of the CDR2 of the VH domain of the anti-hCTLA4 antibody designated 12G4

<400>  70

Val Ile Trp Tyr Asn Thr Ser Lys Lys Asp Tyr Ala Asp Ser Ala Lys 
1               5                   10                  15      


Gly 
    


<210>  71
<211>  321
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  DNA sequence encoding the VL domain of the anti-hCTLA4 antibody designated 12G4

<400>  71
gagattgttc tgacacagag tcctggtaca ttatctttgt cccctggtga aagggcaact       60

ctatcttgta gggcctctca atctattacc agctacttgg cttggtatca acaaaaacca      120

ggtcaagcgc cgagaccatt gatttatggt gtctcctcta gagcaacagg gataccagac      180

agatttagtg gaagcggttc aggtactgat ttcactctaa cgattagccg tttagaacct      240

gaagattttg cagtgtacta ttgtcaacag tacggcagat acccctttac ctttggtcct      300

ggaactaaag tggatataaa g                                                321


<210>  72
<211>  107
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Amino acid sequence of the VL domain of the anti-hCTLA4 antibody designated 12G4

<400>  72

Glu Ile Val Leu Thr Gln Ser Pro Gly Thr Leu Ser Leu Ser Pro Gly 
1               5                   10                  15      


Glu Arg Ala Thr Leu Ser Cys Arg Ala Ser Gln Ser Ile Thr Ser Tyr 
            20                  25                  30          


Leu Ala Trp Tyr Gln Gln Lys Pro Gly Gln Ala Pro Arg Pro Leu Ile 
        35                  40                  45              


Tyr Gly Val Ser Ser Arg Ala Thr Gly Ile Pro Asp Arg Phe Ser Gly 
    50                  55                  60                  


Ser Gly Ser Gly Thr Asp Phe Thr Leu Thr Ile Ser Arg Leu Glu Pro 
65                  70                  75                  80  


Glu Asp Phe Ala Val Tyr Tyr Cys Gln Gln Tyr Gly Arg Tyr Pro Phe 
                85                  90                  95      


Thr Phe Gly Pro Gly Thr Lys Val Asp Ile Lys 
            100                 105         


<210>  73
<211>  11
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Amino acid sequence of the CDR1 of the VL domain of the anti-hCTLA4 antibody designated 12G4

<400>  73

Arg Ala Ser Gln Ser Ile Thr Ser Tyr Leu Ala 
1               5                   10      


<210>  74
<211>  17
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Consensus amino acid sequence for anti-hCTLA4 VH CDR2s


<220>
<221>  X
<222>  (5)..(5)
<223>  X is asparagine, lysine, arginine, glutamine, or histidine

<220>
<221>  X
<222>  (6)..(6)
<223>  X is proline, alanine, serine, threonine, lysine, glutamine, 
       asparagine, or arginine

<220>
<221>  X
<222>  (8)..(8)
<223>  X is glutamate, aspartate, arginine, or lysine

<220>
<221>  X
<222>  (10)..(10)
<223>  X is aspartate, glutamate, tryptophan, phenylalanine, threonine, 
       serine, or tyrosine

<400>  74

Val Ile Trp Tyr Xaa Xaa Ser Xaa Lys Xaa Tyr Ala Asp Ser Ala Lys 
1               5                   10                  15      


Gly 
    


<210>  75
<211>  17
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Second consensus amino acid sequence for anti-hCTLA4 VH CDR2s


<220>
<221>  X
<222>  (5)..(5)
<223>  X is asparagine, lysine, glutamine, or histidine

<220>
<221>  X
<222>  (6)..(6)
<223>  X is proline, serine, threonine, or arginine

<220>
<221>  X
<222>  (8)..(8)
<223>  X is glutamate or lysine

<220>
<221>  X
<222>  (10)..(10)
<223>  X is aspartate or tyrosine

<400>  75

Val Ile Trp Tyr Xaa Xaa Ser Xaa Lys Xaa Tyr Ala Asp Ser Ala Lys 
1               5                   10                  15      


Gly 
    


<210>  76
<211>  9
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Consensus amino acid sequence for anti-hCTLA4 VH CDR3s


<220>
<221>  X
<222>  (1)..(1)
<223>  X is alanine, valine, leucine, isoleucine, or glycine

<220>
<221>  X
<222>  (3)..(4)
<223>  X is leucine, isoleucine, valine, methionine, alanine, or 
       phenylalanine

<400>  76

Xaa Gly Xaa Xaa Gly Tyr Phe Asp Tyr 
1               5                   


<210>  77
<211>  9
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Second consensus amino acid sequence for anti-hCTLA4 VH CDR3


<220>
<221>  X
<222>  (1)..(1)
<223>  X is alanine or glycine

<220>
<221>  X
<222>  (3)..(4)
<223>  X is leucine or phenylalanine

<400>  77

Xaa Gly Xaa Xaa Gly Tyr Phe Asp Tyr 
1               5                   


<210>  78
<211>  11
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Consensus amino acid sequence for anti-hCTLA4 VL CDR1s


<220>
<221>  X
<222>  (6)..(6)
<223>  X is isoleucine, valine, leucine, methionine, alanine, or 
       phenylalanine

<220>
<221>  X
<222>  (7)..(7)
<223>  X is serine asparagine, threonine, glutamine, alanine, or 
       cysteine

<400>  78

Arg Ala Ser Gln Ser Xaa Xaa Ser Tyr Leu Ala 
1               5                   10      


<210>  79
<211>  11
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Second consensus amino acid sequence for anti-hCTLA4 VL CDR1s


<220>
<221>  X
<222>  (6)..(6)
<223>  X is isoleucine, valine, or leucine

<220>
<221>  X
<222>  (7)..(7)
<223>  X is serine, asparagine, or threonine

<400>  79

Arg Ala Ser Gln Ser Xaa Xaa Ser Tyr Leu Ala 
1               5                   10      


<210>  80
<211>  9
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Consensus amino acid sequence for anti-hCTLA4 VL CDR3


<220>
<221>  X
<222>  (5)..(5)
<223>  X is methionine, arginine, or isoleucine

<220>
<221>  X
<222>  (6)..(6)
<223>  X is tyrosine, tryptophan, phenylalanine, threonine, or serine

<400>  80

Gln Gln Tyr Gly Xaa Xaa Pro Phe Thr 
1               5                   


<210>  81
<211>  9
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Second consensus amino acid sequence for anti-hCTLA4 VL CDR3s


<220>
<221>  X
<222>  (5)..(5)
<223>  X is methionine, arginine, or isoleucine

<220>
<221>  X
<222>  (6)..(6)
<223>  X is tyrosine or serine

<400>  81

Gln Gln Tyr Gly Xaa Xaa Pro Phe Thr 
1               5                   


<210>  82
<211>  118
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Consensus amino acid sequence for anti-hCTLA4 VH domains


<220>
<221>  X
<222>  (23)..(23)
<223>  X is threonine, alanine, serine, valine, leucine, or isoleucine

<220>
<221>  X
<222>  (54)..(54)
<223>  X is asparagine, histidine, lysine, glutamine, or arginine

<220>
<221>  X
<222>  (55)..(55)
<223>  X is proline, arginine, serine, threonine, lysine, glutamine, 
       asparagine, alanine, or threonine

<220>
<221>  X
<222>  (57)..(57)
<223>  X is glutamate, lysine, aspartate, arginine, glutamine, or 
       asparagine

<220>
<221>  X
<222>  (59)..(59)
<223>  X is aspartate, tyrosine, glutamate, tryptophan, phenylalanine, 
       threonine, or serine

<220>
<221>  X
<222>  (99)..(99)
<223>  X is alanine, glycine, valine, leucine, isoleucine, or proline

<220>
<221>  X
<222>  (101)..(102)
<223>  X is leucine, phenylalanine, isoleucine, valine, methionine, 
       alanine, or tyrosine

<400>  82

Gln Val Gln Leu Val Glu Ser Gly Gly Gly Val Val Glu Pro Gly Arg 
1               5                   10                  15      


Ser Leu Arg Leu Ser Cys Xaa Ala Ser Gly Phe Thr Phe Ser Ser Tyr 
            20                  25                  30          


Gly Met His Trp Val Arg Gln Ala Pro Gly Lys Gly Leu Glu Trp Val 
        35                  40                  45              


Ala Val Ile Trp Tyr Xaa Xaa Ser Xaa Lys Xaa Tyr Ala Asp Ser Ala 
    50                  55                  60                  


Lys Gly Arg Phe Thr Ile Ser Arg Asp Asn Ser Lys Asn Thr Leu Tyr 
65                  70                  75                  80  


Leu Gln Met Asn Ser Leu Arg Ala Glu Asp Thr Ala Val Tyr Tyr Cys 
                85                  90                  95      


Ala Arg Xaa Gly Xaa Xaa Gly Tyr Phe Asp Tyr Trp Gly Gln Gly Thr 
            100                 105                 110         


Leu Val Thr Val Ser Ser 
        115             


<210>  83
<211>  118
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Second consensus amino acid sequence for anti-hCTLA4 VH domains


<220>
<221>  X
<222>  (23)..(23)
<223>  X is threonine or alanine

<220>
<221>  X
<222>  (54)..(54)
<223>  X is asparagine, histidine, lysine, or glutamine

<220>
<221>  X
<222>  (55)..(55)
<223>  X is proline, arginine, serine, or threonine

<220>
<221>  X
<222>  (57)..(57)
<223>  X is glutamate or lysine

<220>
<221>  X
<222>  (59)..(59)
<223>  X is aspartate or tyrosine

<220>
<221>  X
<222>  (99)..(99)
<223>  X is alanine or glycine

<220>
<221>  X
<222>  (101)..(102)
<223>  X is leucine or phenylalanine

<400>  83

Gln Val Gln Leu Val Glu Ser Gly Gly Gly Val Val Glu Pro Gly Arg 
1               5                   10                  15      


Ser Leu Arg Leu Ser Cys Xaa Ala Ser Gly Phe Thr Phe Ser Ser Tyr 
            20                  25                  30          


Gly Met His Trp Val Arg Gln Ala Pro Gly Lys Gly Leu Glu Trp Val 
        35                  40                  45              


Ala Val Ile Trp Tyr Xaa Xaa Ser Xaa Lys Xaa Tyr Ala Asp Ser Ala 
    50                  55                  60                  


Lys Gly Arg Phe Thr Ile Ser Arg Asp Asn Ser Lys Asn Thr Leu Tyr 
65                  70                  75                  80  


Leu Gln Met Asn Ser Leu Arg Ala Glu Asp Thr Ala Val Tyr Tyr Cys 
                85                  90                  95      


Ala Arg Xaa Gly Xaa Xaa Gly Tyr Phe Asp Tyr Trp Gly Gln Gly Thr 
            100                 105                 110         


Leu Val Thr Val Ser Ser 
        115             


<210>  84
<211>  107
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Consensus amino acid sequence for anti-hCTLA4 VL domains


<220>
<221>  X
<222>  (29)..(29)
<223>  X is isoleucine, valine, leucine, methionine, alanine, or 
       phenylalanine

<220>
<221>  X
<222>  (30)..(30)
<223>  X is serine, asparagine, threonine, alanine, or glutamine

<220>
<221>  X
<222>  (46)..(46)
<223>  X is proline, leucine, alanine, isoleucine, valine, methionine, 
       or phenylalanine

<220>
<221>  X
<222>  (93)..(93)
<223>  X is methionine, isoleucine, arginine, leucine, phenylalanine, 
       valine, alanine lysine, glutamine, or asparagine

<220>
<221>  X
<222>  (94)..(94)
<223>  X is serine, tyrosine, threonine, alanine, tryptophan, or 
       phenylalanine

<400>  84

Glu Ile Val Leu Thr Gln Ser Pro Gly Thr Leu Ser Leu Ser Pro Gly 
1               5                   10                  15      


Glu Arg Ala Thr Leu Ser Cys Arg Ala Ser Gln Ser Xaa Xaa Ser Tyr 
            20                  25                  30          


Leu Ala Trp Tyr Gln Gln Lys Pro Gly Gln Ala Pro Arg Xaa Leu Ile 
        35                  40                  45              


Tyr Gly Val Ser Ser Arg Ala Thr Gly Ile Pro Asp Arg Phe Ser Gly 
    50                  55                  60                  


Ser Gly Ser Gly Thr Asp Phe Thr Leu Thr Ile Ser Arg Leu Glu Pro 
65                  70                  75                  80  


Glu Asp Phe Ala Val Tyr Tyr Cys Gln Gln Tyr Gly Xaa Xaa Pro Phe 
                85                  90                  95      


Thr Phe Gly Pro Gly Thr Lys Val Asp Ile Lys 
            100                 105         


<210>  85
<211>  107
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Second consensus amino acid sequence for anti-hCTLA4 VL domains


<220>
<221>  X
<222>  (29)..(29)
<223>  X is isoleucine, valine, or leucine

<220>
<221>  X
<222>  (30)..(30)
<223>  X is serine asparagine, or threonine

<220>
<221>  X
<222>  (46)..(46)
<223>  X is proline or leucine

<220>
<221>  X
<222>  (93)..(93)
<223>  X is methionine, isoleucine, or arginine

<220>
<221>  X
<222>  (94)..(94)
<223>  X is serine or tyrosine

<400>  85

Glu Ile Val Leu Thr Gln Ser Pro Gly Thr Leu Ser Leu Ser Pro Gly 
1               5                   10                  15      


Glu Arg Ala Thr Leu Ser Cys Arg Ala Ser Gln Ser Xaa Xaa Ser Tyr 
            20                  25                  30          


Leu Ala Trp Tyr Gln Gln Lys Pro Gly Gln Ala Pro Arg Xaa Leu Ile 
        35                  40                  45              


Tyr Gly Val Ser Ser Arg Ala Thr Gly Ile Pro Asp Arg Phe Ser Gly 
    50                  55                  60                  


Ser Gly Ser Gly Thr Asp Phe Thr Leu Thr Ile Ser Arg Leu Glu Pro 
65                  70                  75                  80  


Glu Asp Phe Ala Val Tyr Tyr Cys Gln Gln Tyr Gly Xaa Xaa Pro Phe 
                85                  90                  95      


Thr Phe Gly Pro Gly Thr Lys Val Asp Ile Lys 
            100                 105         


<210>  86
<211>  357
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  DNA sequence encoding the VH domain of the anti-hPD1 antibody designated #1

<400>  86
caagttcaat tggttcaatc tggtgctgaa gtcaaaaagc caggtgcctc tgtcaaggtt       60

tcttgtaaag cttccggtta caccttcact aattactgga ttcactgggt tagacaagct      120

ccaggtcaag gtttggaatg gatgggtgag atcgacccat atgattctta tactaattac      180

aatcaaaagt tcaagggtcg tgttactatg accgtagaca aatccactag taccgtttac      240

atggaattat cttccttgag atccgaggac actgctgttt attattgcgc tcgtccaggt      300

tttacctacg gtggtatgga tttttggggt caaggtactt tggttaccgt ttcttct         357


<210>  87
<211>  119
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Amino acid sequence of the VH domain of the anti-hPD1 antibody designated #1

<400>  87

Gln Val Gln Leu Val Gln Ser Gly Ala Glu Val Lys Lys Pro Gly Ala 
1               5                   10                  15      


Ser Val Lys Val Ser Cys Lys Ala Ser Gly Tyr Thr Phe Thr Asn Tyr 
            20                  25                  30          


Trp Ile His Trp Val Arg Gln Ala Pro Gly Gln Gly Leu Glu Trp Met 
        35                  40                  45              


Gly Glu Ile Asp Pro Tyr Asp Ser Tyr Thr Asn Tyr Asn Gln Lys Phe 
    50                  55                  60                  


Lys Gly Arg Val Thr Met Thr Val Asp Lys Ser Thr Ser Thr Val Tyr 
65                  70                  75                  80  


Met Glu Leu Ser Ser Leu Arg Ser Glu Asp Thr Ala Val Tyr Tyr Cys 
                85                  90                  95      


Ala Arg Pro Gly Phe Thr Tyr Gly Gly Met Asp Phe Trp Gly Gln Gly 
            100                 105                 110         


Thr Leu Val Thr Val Ser Ser 
        115                 


<210>  88
<211>  5
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Amino acid sequence of the CDR1 of the VH domain of the anti-hPD1 antibodies designated #1, #6, #7, #8, and #14

<400>  88

Asn Tyr Trp Ile His 
1               5   


<210>  89
<211>  17
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Amino acid sequence of the CDR2 of the VH domain of the anti-hPD1 antibodies designated #1, #2, #3, #6, #7, #8, #9, #13, #14, and #15

<400>  89

Glu Ile Asp Pro Tyr Asp Ser Tyr Thr Asn Tyr Asn Gln Lys Phe Lys 
1               5                   10                  15      


Gly 
    


<210>  90
<211>  10
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Amino acid sequence of the CDR3 of the VH domain of the anti-hPD1 antibodies designated #1, #3, #5, #9, and #12

<400>  90

Pro Gly Phe Thr Tyr Gly Gly Met Asp Phe 
1               5                   10  


<210>  91
<211>  339
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  DNA sequence encoding the VL domain of the anti-hPD1 antibody designated #1

<400>  91
gatattcaaa tgacccaatc cccatcctct ttgtccgctt ctgttggtga cagagtcacc       60

atcacttgta agtcctctca atccttgttt aattctggta accaaaaaaa ctacttggcc      120

tggtaccaac aaaagccagg taaggttcct aaattgttga tttatggtgc ctccactaga      180

gattctggtg tcccatatag attctctggt tctggttccg gtactgactt tactttgact      240

atttcttcct tgcaaccaga agacgtcgcc acctattact gtcaaaatga ccactactac      300

ccatacactt ttggtggtgg tactaaggtt gaaattaaa                             339


<210>  92
<211>  113
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Amino acid sequence of the VL domain of the anti-hPD1 antibody designated #1

<400>  92

Asp Ile Gln Met Thr Gln Ser Pro Ser Ser Leu Ser Ala Ser Val Gly 
1               5                   10                  15      


Asp Arg Val Thr Ile Thr Cys Lys Ser Ser Gln Ser Leu Phe Asn Ser 
            20                  25                  30          


Gly Asn Gln Lys Asn Tyr Leu Ala Trp Tyr Gln Gln Lys Pro Gly Lys 
        35                  40                  45              


Val Pro Lys Leu Leu Ile Tyr Gly Ala Ser Thr Arg Asp Ser Gly Val 
    50                  55                  60                  


Pro Tyr Arg Phe Ser Gly Ser Gly Ser Gly Thr Asp Phe Thr Leu Thr 
65                  70                  75                  80  


Ile Ser Ser Leu Gln Pro Glu Asp Val Ala Thr Tyr Tyr Cys Gln Asn 
                85                  90                  95      


Asp His Tyr Tyr Pro Tyr Thr Phe Gly Gly Gly Thr Lys Val Glu Ile 
            100                 105                 110         


Lys 
    


<210>  93
<211>  17
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Amino acid sequence of the CDR1 of the VL domain of the anti-hPD1 antibodies designated #1, #3, and #14

<400>  93

Lys Ser Ser Gln Ser Leu Phe Asn Ser Gly Asn Gln Lys Asn Tyr Leu 
1               5                   10                  15      


Ala 
    


<210>  94
<211>  7
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Amino acid sequence of the CDR2 of the VL domain of the anti-hPD1 antibodies designated #1, #2, #4, #5, #8, #9, #10, #11, #12, #13, and #14

<400>  94

Gly Ala Ser Thr Arg Asp Ser 
1               5           


<210>  95
<211>  9
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Amino acid sequence of the CDR3 of the VL domain of the anti-hPD1 antibodies designated #1, #2, #3, #4, #5, #6, #13, #14, and #15

<400>  95

Gln Asn Asp His Tyr Tyr Pro Tyr Thr 
1               5                   


<210>  96
<211>  357
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  DNA sequence encoding the VH domain of the anti-hPD1 antibody designated #2

<400>  96
caagttcaat tggttcaatc tggtgctgaa gtcaaaaagc caggtgcctc tgtcaaggtt       60

tcttgtaaag cttccggtta caccttcact tcttactgga tgcactgggt tagacaagct      120

ccaggtcaag gtttggaatg gatgggtgag atcgacccat atgattctta tactaattac      180

aatcaaaagt tcaagggtcg tgttactatg accgtagaca aatccactag taccgtttac      240

atggaattat cttccttgag atccgaggac actgctgttt attattgcgc tcgtccaggt      300

aatacctacg gtggtatgga ttattggggt caaggtactt tggttaccgt ttcttct         357


<210>  97
<211>  119
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Amino acid sequence of the VH domain of the anti-hPD1 antibody designated #2

<400>  97

Gln Val Gln Leu Val Gln Ser Gly Ala Glu Val Lys Lys Pro Gly Ala 
1               5                   10                  15      


Ser Val Lys Val Ser Cys Lys Ala Ser Gly Tyr Thr Phe Thr Ser Tyr 
            20                  25                  30          


Trp Met His Trp Val Arg Gln Ala Pro Gly Gln Gly Leu Glu Trp Met 
        35                  40                  45              


Gly Glu Ile Asp Pro Tyr Asp Ser Tyr Thr Asn Tyr Asn Gln Lys Phe 
    50                  55                  60                  


Lys Gly Arg Val Thr Met Thr Val Asp Lys Ser Thr Ser Thr Val Tyr 
65                  70                  75                  80  


Met Glu Leu Ser Ser Leu Arg Ser Glu Asp Thr Ala Val Tyr Tyr Cys 
                85                  90                  95      


Ala Arg Pro Gly Asn Thr Tyr Gly Gly Met Asp Tyr Trp Gly Gln Gly 
            100                 105                 110         


Thr Leu Val Thr Val Ser Ser 
        115                 


<210>  98
<211>  5
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Amino acid sequence of the CDR1 of the VH domain of the anti-hPD1 antibodies designated #2, #9, and #10

<400>  98

Ser Tyr Trp Met His 
1               5   


<210>  99
<211>  10
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Amino acid sequence of the CDR3 of the VH domain of the anti-hPD1 antibody designated #2

<400>  99

Pro Gly Asn Thr Tyr Gly Gly Met Asp Tyr 
1               5                   10  


<210>  100
<211>  339
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  DNA sequence encoding the VL domain of the anti-hPD1 antibody designated #2

<400>  100
gatattcaaa tgacccaatc cccatcctct ttgtccgctt ctgttggtga cagagtcacc       60

atcacttgta ggtcctctca atccttgttt aattctggta accaaaaaaa ctacttggcc      120

tggtaccaac aaaagccagg taaggttcct aaattgttga tttatggtgc ctccactaga      180

gattctggtg tcccatatag attctctggt tctggttccg gtactgactt tactttgact      240

atttcttcct tgcaaccaga agacgtcgcc acctattact gtcaaaatga ccactactac      300

ccatacactt ttggtggtgg tactaaggtt gaaattaaa                             339


<210>  101
<211>  113
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Amino acid sequence of the VL domain of the anti-hPD1 antibody designated #2

<400>  101

Asp Ile Gln Met Thr Gln Ser Pro Ser Ser Leu Ser Ala Ser Val Gly 
1               5                   10                  15      


Asp Arg Val Thr Ile Thr Cys Arg Ser Ser Gln Ser Leu Phe Asn Ser 
            20                  25                  30          


Gly Asn Gln Lys Asn Tyr Leu Ala Trp Tyr Gln Gln Lys Pro Gly Lys 
        35                  40                  45              


Val Pro Lys Leu Leu Ile Tyr Gly Ala Ser Thr Arg Asp Ser Gly Val 
    50                  55                  60                  


Pro Tyr Arg Phe Ser Gly Ser Gly Ser Gly Thr Asp Phe Thr Leu Thr 
65                  70                  75                  80  


Ile Ser Ser Leu Gln Pro Glu Asp Val Ala Thr Tyr Tyr Cys Gln Asn 
                85                  90                  95      


Asp His Tyr Tyr Pro Tyr Thr Phe Gly Gly Gly Thr Lys Val Glu Ile 
            100                 105                 110         


Lys 
    


<210>  102
<211>  17
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Amino acid sequence of the CDR1 of the VL domain of the anti-hPD1 antibodies designated #2, #6, and #15

<400>  102

Arg Ser Ser Gln Ser Leu Phe Asn Ser Gly Asn Gln Lys Asn Tyr Leu 
1               5                   10                  15      


Ala 
    


<210>  103
<211>  357
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  DNA sequence encoding the VH domain of the anti-hPD1 antibody designated #3

<400>  103
caagttcaat tggttcaatc tggtgctgaa gtcaaaaagc caggtgcctc tgtcaaggtt       60

tcttgtaaag cttccagtta caccttcact aattactgga tgcactgggt tagacaagct      120

ccaggtcaag gtttggaatg gatgggtgag atcgacccat atgattctta tactaattac      180

aatcaaaagt tcaagggtcg tgttactatg accatagaca catccactag taccgtttac      240

atggaattat cttccttgac atccgaggac actgctgttt attattgcgc tcgtccaggt      300

tttacctacg gtggtatgga tttttggggt caaggtactt tggttaccgt ttcttct         357


<210>  104
<211>  119
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Amino acid sequence of the VH domain of the anti-hPD1 antibody designated #3

<400>  104

Gln Val Gln Leu Val Gln Ser Gly Ala Glu Val Lys Lys Pro Gly Ala 
1               5                   10                  15      


Ser Val Lys Val Ser Cys Lys Ala Ser Ser Tyr Thr Phe Thr Asn Tyr 
            20                  25                  30          


Trp Met His Trp Val Arg Gln Ala Pro Gly Gln Gly Leu Glu Trp Met 
        35                  40                  45              


Gly Glu Ile Asp Pro Tyr Asp Ser Tyr Thr Asn Tyr Asn Gln Lys Phe 
    50                  55                  60                  


Lys Gly Arg Val Thr Met Thr Ile Asp Thr Ser Thr Ser Thr Val Tyr 
65                  70                  75                  80  


Met Glu Leu Ser Ser Leu Thr Ser Glu Asp Thr Ala Val Tyr Tyr Cys 
                85                  90                  95      


Ala Arg Pro Gly Phe Thr Tyr Gly Gly Met Asp Phe Trp Gly Gln Gly 
            100                 105                 110         


Thr Leu Val Thr Val Ser Ser 
        115                 


<210>  105
<211>  5
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Amino acid sequence of the CDR1 of the VH domain of the anti-hPD1 antibodies designated #3, #4, #5, #12, and #15

<400>  105

Asn Tyr Trp Met His 
1               5   


<210>  106
<211>  339
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  DNA sequence encoding the VL domain of the anti-hPD1 antibody designated #3

<400>  106
gatattcaaa tgacccaatc cccatcctct ttgtccgctt ctgttggtga cagagtcacc       60

atcacttgta agtcctctca atccttgttt aattctggta accaaaaaaa ctacttggcc      120

tggtaccaac aaaagccagg taaggttcct aaattgttga tttatggtgc ctccactaga      180

gaatctggtg tcccagctag attctctggt tctggttccg gtactgactt tactttgact      240

atttcttcct tgcaagcaga agacgtcgcc acctattact gtcaaaatga ccactactac      300

ccatacactt ttggtggtgg tactaaggtt gaaattaaa                             339


<210>  107
<211>  113
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Amino acid sequence of the VL domain of the anti-hPD1 antibody designated #3

<400>  107

Asp Ile Gln Met Thr Gln Ser Pro Ser Ser Leu Ser Ala Ser Val Gly 
1               5                   10                  15      


Asp Arg Val Thr Ile Thr Cys Lys Ser Ser Gln Ser Leu Phe Asn Ser 
            20                  25                  30          


Gly Asn Gln Lys Asn Tyr Leu Ala Trp Tyr Gln Gln Lys Pro Gly Lys 
        35                  40                  45              


Val Pro Lys Leu Leu Ile Tyr Gly Ala Ser Thr Arg Glu Ser Gly Val 
    50                  55                  60                  


Pro Ala Arg Phe Ser Gly Ser Gly Ser Gly Thr Asp Phe Thr Leu Thr 
65                  70                  75                  80  


Ile Ser Ser Leu Gln Ala Glu Asp Val Ala Thr Tyr Tyr Cys Gln Asn 
                85                  90                  95      


Asp His Tyr Tyr Pro Tyr Thr Phe Gly Gly Gly Thr Lys Val Glu Ile 
            100                 105                 110         


Lys 
    


<210>  108
<211>  7
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Amino acid sequence of the CDR2 of the VL domain of the anti-hPD1 antibodies designated #3, #6, #7, and #15

<400>  108

Gly Ala Ser Thr Arg Glu Ser 
1               5           


<210>  109
<211>  357
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  DNA sequence encoding the VH domain of the anti-hPD1 antibody designated #4

<400>  109
caagttcaat tggttcaatc tggtgctgaa gtcaaaaagc caggtgcctc tgtcaaggtt       60

tcttgtaaag cttccggtta caccttcact aattactgga tgcactgggt tagacaagct      120

ccaggtcaag gtttggaatg gatgggtgag atcgacccat ttgattctta tactaattac      180

aatcaaaagt tcaagggtcg tgttactatg accagagaca catccactag taccgtttac      240

atggaattat cttccttgac atccgaggac actgctgttt attattgcgc tcgtccaggt      300

tatacctacg gtggtatgga tttttggggt caaggtactt tggttaccgt ttcttct         357


<210>  110
<211>  119
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Amino acid sequence of the VH domain of the anti-hPD1 antibody designated #4

<400>  110

Gln Val Gln Leu Val Gln Ser Gly Ala Glu Val Lys Lys Pro Gly Ala 
1               5                   10                  15      


Ser Val Lys Val Ser Cys Lys Ala Ser Gly Tyr Thr Phe Thr Asn Tyr 
            20                  25                  30          


Trp Met His Trp Val Arg Gln Ala Pro Gly Gln Gly Leu Glu Trp Met 
        35                  40                  45              


Gly Glu Ile Asp Pro Phe Asp Ser Tyr Thr Asn Tyr Asn Gln Lys Phe 
    50                  55                  60                  


Lys Gly Arg Val Thr Met Thr Arg Asp Thr Ser Thr Ser Thr Val Tyr 
65                  70                  75                  80  


Met Glu Leu Ser Ser Leu Thr Ser Glu Asp Thr Ala Val Tyr Tyr Cys 
                85                  90                  95      


Ala Arg Pro Gly Tyr Thr Tyr Gly Gly Met Asp Phe Trp Gly Gln Gly 
            100                 105                 110         


Thr Leu Val Thr Val Ser Ser 
        115                 


<210>  111
<211>  17
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Amino acid sequence of the CDR2 of the VH domain of the anti-hPD1 antibodies designated #4, #5, #10, and #12

<400>  111

Glu Ile Asp Pro Phe Asp Ser Tyr Thr Asn Tyr Asn Gln Lys Phe Lys 
1               5                   10                  15      


Gly 
    


<210>  112
<211>  10
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Amino acid sequence of the CDR3 of the VH domain of the anti-hPD1 antibodies designated #4, #6, #10, #11, and #14

<400>  112

Pro Gly Tyr Thr Tyr Gly Gly Met Asp Phe 
1               5                   10  


<210>  113
<211>  339
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  DNA sequence encoding the VL domain of the anti-hPD1 antibody designated #4

<400>  113
gatattcaaa tgacccaatc cccatcctct ttgtccgctt ctgttggtga cagagtcacc       60

atcacttgta agtcctctca atccttgttg aattctggta accaaaaaaa ctacttggcc      120

tggtaccaac aaaagccagg taagcctcct aaattgttga tttatggtgc ctccactaga      180

gattctggtg tcccatctag attctctggt tctggttccg gtactgactt tactttgact      240

atttcttcct tgcaaccaga agacgtcgcc acctattact gtcaaaatga ccactactac      300

ccatacactt ttggtggtgg tactaaggtt gaaattaaa                             339


<210>  114
<211>  113
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Amino acid sequence of the VL domain of the anti-hPD1 antibody designated #4

<400>  114

Asp Ile Gln Met Thr Gln Ser Pro Ser Ser Leu Ser Ala Ser Val Gly 
1               5                   10                  15      


Asp Arg Val Thr Ile Thr Cys Lys Ser Ser Gln Ser Leu Leu Asn Ser 
            20                  25                  30          


Gly Asn Gln Lys Asn Tyr Leu Ala Trp Tyr Gln Gln Lys Pro Gly Lys 
        35                  40                  45              


Pro Pro Lys Leu Leu Ile Tyr Gly Ala Ser Thr Arg Asp Ser Gly Val 
    50                  55                  60                  


Pro Ser Arg Phe Ser Gly Ser Gly Ser Gly Thr Asp Phe Thr Leu Thr 
65                  70                  75                  80  


Ile Ser Ser Leu Gln Pro Glu Asp Val Ala Thr Tyr Tyr Cys Gln Asn 
                85                  90                  95      


Asp His Tyr Tyr Pro Tyr Thr Phe Gly Gly Gly Thr Lys Val Glu Ile 
            100                 105                 110         


Lys 
    


<210>  115
<211>  17
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Amino acid sequence of the CDR1 of the VL domain of the anti-hPD1 antibodies designated #4 and #13

<400>  115

Lys Ser Ser Gln Ser Leu Leu Asn Ser Gly Asn Gln Lys Asn Tyr Leu 
1               5                   10                  15      


Ala 
    


<210>  116
<211>  357
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  DNA sequence encoding the VH domain of the anti-hPD1 antibody designated #5

<400>  116
caagttcaat tggttcaatc tggtgctgaa gtcaaaaagc caggtgcctc tgtcaaggtt       60

tcttgtaaag cttccggtta caccttcact aattactgga tgcactgggt tagacaagct      120

ccaggtcaag gtttggaatg gatgggtgag atcgacccat ttgattctta tactaattac      180

aatcaaaagt tcaagggtcg tgttactatg accagagaca aatccactag taccgtttac      240

atggaattat cttccttgag atccgaggac actgctgttt attattgcgc tcgtccaggt      300

tttacctacg gtggtatgga tttttggggt caaggtactt tggttaccgt ttcttct         357


<210>  117
<211>  119
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Amino acid sequence of the VH domain of the anti-hPD1 antibody designated #5

<400>  117

Gln Val Gln Leu Val Gln Ser Gly Ala Glu Val Lys Lys Pro Gly Ala 
1               5                   10                  15      


Ser Val Lys Val Ser Cys Lys Ala Ser Gly Tyr Thr Phe Thr Asn Tyr 
            20                  25                  30          


Trp Met His Trp Val Arg Gln Ala Pro Gly Gln Gly Leu Glu Trp Met 
        35                  40                  45              


Gly Glu Ile Asp Pro Phe Asp Ser Tyr Thr Asn Tyr Asn Gln Lys Phe 
    50                  55                  60                  


Lys Gly Arg Val Thr Met Thr Arg Asp Lys Ser Thr Ser Thr Val Tyr 
65                  70                  75                  80  


Met Glu Leu Ser Ser Leu Arg Ser Glu Asp Thr Ala Val Tyr Tyr Cys 
                85                  90                  95      


Ala Arg Pro Gly Phe Thr Tyr Gly Gly Met Asp Phe Trp Gly Gln Gly 
            100                 105                 110         


Thr Leu Val Thr Val Ser Ser 
        115                 


<210>  118
<211>  339
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  DNA sequence encoding the VL domain of the anti-hPD1 antibody designated #5

<400>  118
gatattcaaa tgacccaatc cccatcctct ttgtccgctt ctgttggtga cagagtcacc       60

atcacttgta gatcctctca atccttgttg aattctggta accaaaaaaa ctacttggcc      120

tggtaccaac aaaagccagg taagcttcct aaattgttga tttatggtgc ctccactaga      180

gattctggtg tcccatctag attctctggt tctggttccg gtactgactt tactttgact      240

atttcttcct tgcaagcaga agacgtcgcc acctattact gtcaaaatga ccactactac      300

ccatacactt ttggtggtgg tactaaggtt gaaattaaa                             339


<210>  119
<211>  113
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Amino acid sequence of the VL domain of the anti-hPD1 antibody designated #5

<400>  119

Asp Ile Gln Met Thr Gln Ser Pro Ser Ser Leu Ser Ala Ser Val Gly 
1               5                   10                  15      


Asp Arg Val Thr Ile Thr Cys Arg Ser Ser Gln Ser Leu Leu Asn Ser 
            20                  25                  30          


Gly Asn Gln Lys Asn Tyr Leu Ala Trp Tyr Gln Gln Lys Pro Gly Lys 
        35                  40                  45              


Leu Pro Lys Leu Leu Ile Tyr Gly Ala Ser Thr Arg Asp Ser Gly Val 
    50                  55                  60                  


Pro Ser Arg Phe Ser Gly Ser Gly Ser Gly Thr Asp Phe Thr Leu Thr 
65                  70                  75                  80  


Ile Ser Ser Leu Gln Ala Glu Asp Val Ala Thr Tyr Tyr Cys Gln Asn 
                85                  90                  95      


Asp His Tyr Tyr Pro Tyr Thr Phe Gly Gly Gly Thr Lys Val Glu Ile 
            100                 105                 110         


Lys 
    


<210>  120
<211>  17
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Amino acid sequence of the CDR1 of the VL domain of the anti-hPD1 antibody designated #5

<400>  120

Arg Ser Ser Gln Ser Leu Leu Asn Ser Gly Asn Gln Lys Asn Tyr Leu 
1               5                   10                  15      


Ala 
    


<210>  121
<211>  357
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  DNA sequence encoding the VH domain of the anti-hPD1 antibody designated #6

<400>  121
caagttcaat tggttcaatc tggtgctgaa gtcaaaaagc caggtgcctc tgtcaaggtt       60

tcttgtaaag cttccggtta caccttcact aattactgga ttcactgggt tagacaagct      120

ccaggtcaag gtttggaatg gatgggtgag atcgacccat atgattctta tactaattac      180

aatcaaaagt tcaagggtcg tgttactatg accggagaca catccactag taccgtttac      240

atggaattat cttccttgac atccgaggac actgctgttt attattgcgc tcgtccaggt      300

tatacctacg gtggtatgga tttttggggt caaggtactt tggttaccgt ttcttct         357


<210>  122
<211>  119
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Amino acid sequence of the VH domain of the anti-hPD1 antibody designated #6

<400>  122

Gln Val Gln Leu Val Gln Ser Gly Ala Glu Val Lys Lys Pro Gly Ala 
1               5                   10                  15      


Ser Val Lys Val Ser Cys Lys Ala Ser Gly Tyr Thr Phe Thr Asn Tyr 
            20                  25                  30          


Trp Ile His Trp Val Arg Gln Ala Pro Gly Gln Gly Leu Glu Trp Met 
        35                  40                  45              


Gly Glu Ile Asp Pro Tyr Asp Ser Tyr Thr Asn Tyr Asn Gln Lys Phe 
    50                  55                  60                  


Lys Gly Arg Val Thr Met Thr Gly Asp Thr Ser Thr Ser Thr Val Tyr 
65                  70                  75                  80  


Met Glu Leu Ser Ser Leu Thr Ser Glu Asp Thr Ala Val Tyr Tyr Cys 
                85                  90                  95      


Ala Arg Pro Gly Tyr Thr Tyr Gly Gly Met Asp Phe Trp Gly Gln Gly 
            100                 105                 110         


Thr Leu Val Thr Val Ser Ser 
        115                 


<210>  123
<211>  339
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  DNA sequence encoding the VL domain of the anti-hPD1 antibody designated #6

<400>  123
gatattcaaa tgacccaatc cccatcctct ttgtccgctt ctgttggtga cagagtcacc       60

atcacttgta ggtcctctca atccttgttt aattctggta accaaaaaaa ctacttggcc      120

tggtaccaac aaaagccagg taagcctcct aaattgttga tttatggtgc ctccactaga      180

gaatctggtg tcccagctag attctctggt tctggttccg gtactgactt tactttgact      240

atttcttcct tgcaaccaga agacgtcgcc acctattact gtcaaaatga ccactactac      300

ccatacactt ttggtggtgg tactaaggtt gaaattaaa                             339


<210>  124
<211>  113
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Amino acid sequence of the VL domain of the anti-hPD1 antibody designated #6

<400>  124

Asp Ile Gln Met Thr Gln Ser Pro Ser Ser Leu Ser Ala Ser Val Gly 
1               5                   10                  15      


Asp Arg Val Thr Ile Thr Cys Arg Ser Ser Gln Ser Leu Phe Asn Ser 
            20                  25                  30          


Gly Asn Gln Lys Asn Tyr Leu Ala Trp Tyr Gln Gln Lys Pro Gly Lys 
        35                  40                  45              


Pro Pro Lys Leu Leu Ile Tyr Gly Ala Ser Thr Arg Glu Ser Gly Val 
    50                  55                  60                  


Pro Ala Arg Phe Ser Gly Ser Gly Ser Gly Thr Asp Phe Thr Leu Thr 
65                  70                  75                  80  


Ile Ser Ser Leu Gln Pro Glu Asp Val Ala Thr Tyr Tyr Cys Gln Asn 
                85                  90                  95      


Asp His Tyr Tyr Pro Tyr Thr Phe Gly Gly Gly Thr Lys Val Glu Ile 
            100                 105                 110         


Lys 
    


<210>  125
<211>  357
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  DNA sequence encoding the VH domain of the anti-hPD1 antibody designated #7

<400>  125
caagttcaat tggttcaatc tggtgctgaa gtcaaaaagc caggtgcctc tgtcaaggtt       60

tcttgtaaag cttccggtta caccttcact aattactgga ttcactgggt tagacaagct      120

ccaggtcaag gtttggaatg gatgggtgag atcgacccat atgattctta tactaattac      180

aatcaaaagt tcaagggtcg tgttactatg accatagaca aatccactaa taccgtttac      240

atggaattat cttccttgag atccgaggac actgctgttt attattgcgc tcgtccaggt      300

cttacctacg gtggtatgga tttttggggt caaggtactt tggttaccgt ttcttct         357


<210>  126
<211>  119
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Amino acid sequence of the VH domain of the anti-hPD1 antibody designated #7

<400>  126

Gln Val Gln Leu Val Gln Ser Gly Ala Glu Val Lys Lys Pro Gly Ala 
1               5                   10                  15      


Ser Val Lys Val Ser Cys Lys Ala Ser Gly Tyr Thr Phe Thr Asn Tyr 
            20                  25                  30          


Trp Ile His Trp Val Arg Gln Ala Pro Gly Gln Gly Leu Glu Trp Met 
        35                  40                  45              


Gly Glu Ile Asp Pro Tyr Asp Ser Tyr Thr Asn Tyr Asn Gln Lys Phe 
    50                  55                  60                  


Lys Gly Arg Val Thr Met Thr Ile Asp Lys Ser Thr Asn Thr Val Tyr 
65                  70                  75                  80  


Met Glu Leu Ser Ser Leu Arg Ser Glu Asp Thr Ala Val Tyr Tyr Cys 
                85                  90                  95      


Ala Arg Pro Gly Leu Thr Tyr Gly Gly Met Asp Phe Trp Gly Gln Gly 
            100                 105                 110         


Thr Leu Val Thr Val Ser Ser 
        115                 


<210>  127
<211>  10
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Amino acid sequence of the CDR3 of the VH domain of the anti-hPD1 antibody designated #7, #13, and #15

<400>  127

Pro Gly Leu Thr Tyr Gly Gly Met Asp Phe 
1               5                   10  


<210>  128
<211>  339
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  DNA sequence encoding the VL domain of the anti-hPD1 antibody designated #7

<400>  128
gatattgtta tgacccaatc cccagattcc ttggctgtct ctttgggtga aagagctact       60

attaattgta gatcttccca atctttgttt atatccggta accaaaagaa ctatttggcc      120

tggtatcaac aaaagccagg tcaaccacct aagttattga tctacggtgc ttccaccaga      180

gaatctggtg ttccagatcg tttctctggt tctggttctg gtactgattt caccttaact      240

atttcttctt tgcaagccga agacgtcgct gtttactact gtcaaaacaa ccattattac      300

ccttacacct tcggtggtgg taccaaggtc gaaattaag                             339


<210>  129
<211>  113
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Amino acid sequence of the VL domain of the anti-hPD1 antibody designated #7

<400>  129

Asp Ile Val Met Thr Gln Ser Pro Asp Ser Leu Ala Val Ser Leu Gly 
1               5                   10                  15      


Glu Arg Ala Thr Ile Asn Cys Arg Ser Ser Gln Ser Leu Phe Ile Ser 
            20                  25                  30          


Gly Asn Gln Lys Asn Tyr Leu Ala Trp Tyr Gln Gln Lys Pro Gly Gln 
        35                  40                  45              


Pro Pro Lys Leu Leu Ile Tyr Gly Ala Ser Thr Arg Glu Ser Gly Val 
    50                  55                  60                  


Pro Asp Arg Phe Ser Gly Ser Gly Ser Gly Thr Asp Phe Thr Leu Thr 
65                  70                  75                  80  


Ile Ser Ser Leu Gln Ala Glu Asp Val Ala Val Tyr Tyr Cys Gln Asn 
                85                  90                  95      


Asn His Tyr Tyr Pro Tyr Thr Phe Gly Gly Gly Thr Lys Val Glu Ile 
            100                 105                 110         


Lys 
    


<210>  130
<211>  17
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Amino acid sequence of the CDR1 of the VL domain of the anti-hPD1 antibodies designated #7, #10, and #11

<400>  130

Arg Ser Ser Gln Ser Leu Phe Ile Ser Gly Asn Gln Lys Asn Tyr Leu 
1               5                   10                  15      


Ala 
    


<210>  131
<211>  9
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Amino acid sequence of the CDR3 of the VL domain of the anti-hPD1 antibodies designated #7, #8, #9, #10,  #11, and #12

<400>  131

Gln Asn Asn His Tyr Tyr Pro Tyr Thr 
1               5                   


<210>  132
<211>  357
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  DNA sequence encoding the VH domain of the anti-hPD1 antibody designated #8

<400>  132
caagttcaat tggttcaatc tggtgctgaa gtcaaaaagc caggtgcctc tgtcaaggtt       60

tcttgtaaag cttccggtta caccttcact aattactgga ttcactgggt tagacaagct      120

ccaggtcaag gtttggaatg gatgggtgag atcgacccgt atgattctta tactaattac      180

aatcaaaagt tcaagggtcg tgttactatg accatagaca catccactag taccgtttac      240

atggaattat cttccttgag atccgaggac actgctgttt attattgcgc tcgtccaggt      300

aatacctacg gtggtatgga tttttggggt caaggtactt tggttaccgt ttcttct         357


<210>  133
<211>  119
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Amino acid sequence of the VH domain of the anti-hPD1 antibody designated #8

<400>  133

Gln Val Gln Leu Val Gln Ser Gly Ala Glu Val Lys Lys Pro Gly Ala 
1               5                   10                  15      


Ser Val Lys Val Ser Cys Lys Ala Ser Gly Tyr Thr Phe Thr Asn Tyr 
            20                  25                  30          


Trp Ile His Trp Val Arg Gln Ala Pro Gly Gln Gly Leu Glu Trp Met 
        35                  40                  45              


Gly Glu Ile Asp Pro Tyr Asp Ser Tyr Thr Asn Tyr Asn Gln Lys Phe 
    50                  55                  60                  


Lys Gly Arg Val Thr Met Thr Ile Asp Thr Ser Thr Ser Thr Val Tyr 
65                  70                  75                  80  


Met Glu Leu Ser Ser Leu Arg Ser Glu Asp Thr Ala Val Tyr Tyr Cys 
                85                  90                  95      


Ala Arg Pro Gly Asn Thr Tyr Gly Gly Met Asp Phe Trp Gly Gln Gly 
            100                 105                 110         


Thr Leu Val Thr Val Ser Ser 
        115                 


<210>  134
<211>  10
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Amino acid sequence of the CDR3 of the VH domain of the anti-hPD1 antibody designated #8

<400>  134

Pro Gly Asn Thr Tyr Gly Gly Met Asp Phe 
1               5                   10  


<210>  135
<211>  339
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  DNA sequence encoding the VL domain of the anti-hPD1 antibody designated #8

<400>  135
gatattgtta tgacccaatc cccagattcc ttggctgtct ctttgggtga aagagctact       60

attaattgta aatcttccca atctttgttt atatccggta accaaaagaa ctatttggcc      120

tggtatcaac aaaagccagg tcaaccacct aagttattga tctacggtgc ttccaccaga      180

gattctggtg ttccagatcg tttctctggt tctggttctg gtactgattt caccttaact      240

atttcttctt tgcaagccga agacgtcgct gtttactact gtcaaaacaa ccattattac      300

ccttacacct tcggtggtgg taccaaggtc gaaattaag                             339


<210>  136
<211>  113
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Amino acid sequence of the VL domain of the anti-hPD1 antibody designated #8

<400>  136

Asp Ile Val Met Thr Gln Ser Pro Asp Ser Leu Ala Val Ser Leu Gly 
1               5                   10                  15      


Glu Arg Ala Thr Ile Asn Cys Lys Ser Ser Gln Ser Leu Phe Ile Ser 
            20                  25                  30          


Gly Asn Gln Lys Asn Tyr Leu Ala Trp Tyr Gln Gln Lys Pro Gly Gln 
        35                  40                  45              


Pro Pro Lys Leu Leu Ile Tyr Gly Ala Ser Thr Arg Asp Ser Gly Val 
    50                  55                  60                  


Pro Asp Arg Phe Ser Gly Ser Gly Ser Gly Thr Asp Phe Thr Leu Thr 
65                  70                  75                  80  


Ile Ser Ser Leu Gln Ala Glu Asp Val Ala Val Tyr Tyr Cys Gln Asn 
                85                  90                  95      


Asn His Tyr Tyr Pro Tyr Thr Phe Gly Gly Gly Thr Lys Val Glu Ile 
            100                 105                 110         


Lys 
    


<210>  137
<211>  17
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Amino acid sequence of the CDR1 of the VL domain of the anti-hPD1 antibody designated #8

<400>  137

Lys Ser Ser Gln Ser Leu Phe Ile Ser Gly Asn Gln Lys Asn Tyr Leu 
1               5                   10                  15      


Ala 
    


<210>  138
<211>  357
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  DNA sequence encoding the VH domain of the anti-hPD1 antibody designated #9

<400>  138
caagttcaat tggttcaatc tggtgctgaa gtcaaaaagc caggtgcctc tgtcaaggtt       60

tcttgtaaag cttccggtta caccttcact tcttactgga tgcactgggt tagacaagct      120

ccaggtcaag gtttggaatg gatgggtgag atcgacccat atgattctta tactaattac      180

aatcaaaagt tcaagggtcg tgttactatg accatagaca aatccactag taccgtttac      240

atggaattat cttccttgac atccgaggac actgctgttt attattgcgc tcgtccaggt      300

tttacctacg gtggtatgga tttttggggt caaggtactt tggttaccgt ttcttct         357


<210>  139
<211>  119
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Amino acid sequence of the VH domain of the anti-hPD1 antibody designated #9

<400>  139

Gln Val Gln Leu Val Gln Ser Gly Ala Glu Val Lys Lys Pro Gly Ala 
1               5                   10                  15      


Ser Val Lys Val Ser Cys Lys Ala Ser Gly Tyr Thr Phe Thr Ser Tyr 
            20                  25                  30          


Trp Met His Trp Val Arg Gln Ala Pro Gly Gln Gly Leu Glu Trp Met 
        35                  40                  45              


Gly Glu Ile Asp Pro Tyr Asp Ser Tyr Thr Asn Tyr Asn Gln Lys Phe 
    50                  55                  60                  


Lys Gly Arg Val Thr Met Thr Ile Asp Lys Ser Thr Ser Thr Val Tyr 
65                  70                  75                  80  


Met Glu Leu Ser Ser Leu Thr Ser Glu Asp Thr Ala Val Tyr Tyr Cys 
                85                  90                  95      


Ala Arg Pro Gly Phe Thr Tyr Gly Gly Met Asp Phe Trp Gly Gln Gly 
            100                 105                 110         


Thr Leu Val Thr Val Ser Ser 
        115                 


<210>  140
<211>  339
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  DNA sequence encoding the VL domain of the anti-hPD1 antibody designated #9

<400>  140
gatattgtta tgacccaatc cccagattcc ttggctgtct ctttgggtga aagagctact       60

attaattgta gatcttccca atctttgttg atttccggta accaaaagaa ctatttggcc      120

tggtatcaac aaaagccagg tcaaccacct aagttattga tctacggtgc ttccaccaga      180

gattctggtg ttccagatcg tttctctggt tctggttctg gtactgattt caccttaact      240

atttcttctt tgcaagccga agacgtcgct gtttactact gtcaaaacaa ccattattac      300

ccttacacct tcggtggtgg taccaaggtc gaaattaag                             339


<210>  141
<211>  113
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Amino acid sequence of the VL domain of the anti-hPD1 antibody designated #9

<400>  141

Asp Ile Val Met Thr Gln Ser Pro Asp Ser Leu Ala Val Ser Leu Gly 
1               5                   10                  15      


Glu Arg Ala Thr Ile Asn Cys Arg Ser Ser Gln Ser Leu Leu Ile Ser 
            20                  25                  30          


Gly Asn Gln Lys Asn Tyr Leu Ala Trp Tyr Gln Gln Lys Pro Gly Gln 
        35                  40                  45              


Pro Pro Lys Leu Leu Ile Tyr Gly Ala Ser Thr Arg Asp Ser Gly Val 
    50                  55                  60                  


Pro Asp Arg Phe Ser Gly Ser Gly Ser Gly Thr Asp Phe Thr Leu Thr 
65                  70                  75                  80  


Ile Ser Ser Leu Gln Ala Glu Asp Val Ala Val Tyr Tyr Cys Gln Asn 
                85                  90                  95      


Asn His Tyr Tyr Pro Tyr Thr Phe Gly Gly Gly Thr Lys Val Glu Ile 
            100                 105                 110         


Lys 
    


<210>  142
<211>  17
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Amino acid sequence of the CDR1 of the VL domain of the anti-hPD1 antibodies designated #9 and #12

<400>  142

Arg Ser Ser Gln Ser Leu Leu Ile Ser Gly Asn Gln Lys Asn Tyr Leu 
1               5                   10                  15      


Ala 
    


<210>  143
<211>  357
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  DNA sequence encoding the VH domain of the anti-hPD1 antibody designated #10

<400>  143
caagttcaat tggttcaatc tggtgctgaa gtcaaaaagc caggtgcctc tgtcaaggtt       60

tcttgtaaag cttccggtta caccttcact tcttactgga tgcactgggt tagacaagct      120

ccaggtcaag gtttggaatg gatgggtgag atcgacccat ttgattctta tactaattac      180

aatcaaaagt tcaagggtcg tgttactatg accatagaca aatccactaa taccgtttac      240

atggaattat cttccttggg atccgaggac actgctgttt attattgcgc tcgtccaggt      300

tatacctacg gtggtatgga tttttggggt caaggtactt tggttaccgt ttcttct         357


<210>  144
<211>  119
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Amino acid sequence of the VH domain of the anti-hPD1 antibody designated #10

<400>  144

Gln Val Gln Leu Val Gln Ser Gly Ala Glu Val Lys Lys Pro Gly Ala 
1               5                   10                  15      


Ser Val Lys Val Ser Cys Lys Ala Ser Gly Tyr Thr Phe Thr Ser Tyr 
            20                  25                  30          


Trp Met His Trp Val Arg Gln Ala Pro Gly Gln Gly Leu Glu Trp Met 
        35                  40                  45              


Gly Glu Ile Asp Pro Phe Asp Ser Tyr Thr Asn Tyr Asn Gln Lys Phe 
    50                  55                  60                  


Lys Gly Arg Val Thr Met Thr Ile Asp Lys Ser Thr Asn Thr Val Tyr 
65                  70                  75                  80  


Met Glu Leu Ser Ser Leu Gly Ser Glu Asp Thr Ala Val Tyr Tyr Cys 
                85                  90                  95      


Ala Arg Pro Gly Tyr Thr Tyr Gly Gly Met Asp Phe Trp Gly Gln Gly 
            100                 105                 110         


Thr Leu Val Thr Val Ser Ser 
        115                 


<210>  145
<211>  339
<212>  DNA
<213>  Artificial Sequence

<220>
<223> DNA sequence encoding the VL domain of the anti-hPD1 antibody designated #10

<400>  145
gatattgtta tgacccaatc cccagattcc ttggctgtct ctttgggtga aagagctact       60

attaattgta gatcttccca atctttgttt atttccggta accaaaagaa ctatttggcc      120

tggtatcaac aaaagccagg tcaaccacct aagttattga tctacggtgc ttccaccaga      180

gattctggtg ttccagatcg tttctctggt tctggttctg gtactgattt caccttaact      240

atttcttctt tgcaagccga agacgtcgct gtttactact gtcaaaacaa ccattattac      300

ccttacacct tcggtggtgg taccaaggtc gaaattaag                             339


<210>  146
<211>  113
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Amino acid sequence of the VL domain of the anti-hPD1 antibody designated #10

<400>  146

Asp Ile Val Met Thr Gln Ser Pro Asp Ser Leu Ala Val Ser Leu Gly 
1               5                   10                  15      


Glu Arg Ala Thr Ile Asn Cys Arg Ser Ser Gln Ser Leu Phe Ile Ser 
            20                  25                  30          


Gly Asn Gln Lys Asn Tyr Leu Ala Trp Tyr Gln Gln Lys Pro Gly Gln 
        35                  40                  45              


Pro Pro Lys Leu Leu Ile Tyr Gly Ala Ser Thr Arg Asp Ser Gly Val 
    50                  55                  60                  


Pro Asp Arg Phe Ser Gly Ser Gly Ser Gly Thr Asp Phe Thr Leu Thr 
65                  70                  75                  80  


Ile Ser Ser Leu Gln Ala Glu Asp Val Ala Val Tyr Tyr Cys Gln Asn 
                85                  90                  95      


Asn His Tyr Tyr Pro Tyr Thr Phe Gly Gly Gly Thr Lys Val Glu Ile 
            100                 105                 110         


Lys 
    


<210>  147
<211>  357
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  DNA sequence encoding the VH domain of the anti-hPD1 antibody designated #11

<400>  147
caagttcaat tggttcaatc tggtgctgaa gtcaaaaagc caggtgcctc tgtcaaggtt       60

tcttgtaaag cttccggtta caccttcact tcttactgga ttcactgggt tagacaagct      120

ccaggtcaag gtttggaatg gatgggtgag atcgacccat ctgattctta tactaattac      180

aatcaaaagt tcaagggtcg tgttactatg accggagaca catccactaa taccgtttac      240

atggaattat cttccttgac atccgaggac actgctgttt attattgcgc tcgtccaggt      300

tatacctacg gtggtatgga tttttggggt caaggtactt tggttaccgt ttcttct         357


<210>  148
<211>  119
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Amino acid sequence of the VH domain of the anti-hPD1 antibody designated #11

<400>  148

Gln Val Gln Leu Val Gln Ser Gly Ala Glu Val Lys Lys Pro Gly Ala 
1               5                   10                  15      


Ser Val Lys Val Ser Cys Lys Ala Ser Gly Tyr Thr Phe Thr Ser Tyr 
            20                  25                  30          


Trp Ile His Trp Val Arg Gln Ala Pro Gly Gln Gly Leu Glu Trp Met 
        35                  40                  45              


Gly Glu Ile Asp Pro Ser Asp Ser Tyr Thr Asn Tyr Asn Gln Lys Phe 
    50                  55                  60                  


Lys Gly Arg Val Thr Met Thr Gly Asp Thr Ser Thr Asn Thr Val Tyr 
65                  70                  75                  80  


Met Glu Leu Ser Ser Leu Thr Ser Glu Asp Thr Ala Val Tyr Tyr Cys 
                85                  90                  95      


Ala Arg Pro Gly Tyr Thr Tyr Gly Gly Met Asp Phe Trp Gly Gln Gly 
            100                 105                 110         


Thr Leu Val Thr Val Ser Ser 
        115                 


<210>  149
<211>  5
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Amino acid sequence of the CDR1 of the VH domain of the anti-hPD1 antibodies designated #11 and #13

<400>  149

Ser Tyr Trp Ile His 
1               5   


<210>  150
<211>  17
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Amino acid sequence of the CDR2 of the VH domain of the anti-hPD1 antibody designated #11

<400>  150

Glu Ile Asp Pro Ser Asp Ser Tyr Thr Asn Tyr Asn Gln Lys Phe Lys 
1               5                   10                  15      


Gly 
    


<210>  151
<211>  339
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  DNA sequence encoding the VL domain of the anti-hPD1 antibody designated #11

<400>  151
gatattgtta tgacccaatc cccagattcc ttggctgtct ctttgggtga aagagctact       60

attagttgta gatcttccca atctttgttt atatccggta accaaaagaa ctatttggcc      120

tggtatcaac aaaagccagg tcaaccacct aagttattga tctacggtgc ttccaccaga      180

gattctggtg ttccagatcg tttctctggt tctggttctg gtactgattt caccttaact      240

atttcttctt tgcaagccga agacgtcgct gtttactact gtcaaaacaa ccattattac      300

ccttacacct tcggtggtgg taccaaggtc gaaattaag                             339


<210>  152
<211>  113
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Amino acid sequence of the VL domain of the anti-hPD1 antibody designated #11

<400>  152

Asp Ile Val Met Thr Gln Ser Pro Asp Ser Leu Ala Val Ser Leu Gly 
1               5                   10                  15      


Glu Arg Ala Thr Ile Ser Cys Arg Ser Ser Gln Ser Leu Phe Ile Ser 
            20                  25                  30          


Gly Asn Gln Lys Asn Tyr Leu Ala Trp Tyr Gln Gln Lys Pro Gly Gln 
        35                  40                  45              


Pro Pro Lys Leu Leu Ile Tyr Gly Ala Ser Thr Arg Asp Ser Gly Val 
    50                  55                  60                  


Pro Asp Arg Phe Ser Gly Ser Gly Ser Gly Thr Asp Phe Thr Leu Thr 
65                  70                  75                  80  


Ile Ser Ser Leu Gln Ala Glu Asp Val Ala Val Tyr Tyr Cys Gln Asn 
                85                  90                  95      


Asn His Tyr Tyr Pro Tyr Thr Phe Gly Gly Gly Thr Lys Val Glu Ile 
            100                 105                 110         


Lys 
    


<210>  153
<211>  357
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  DNA sequence encoding the VH domain of the anti-hPD1 antibody designated #12

<400>  153
caagttcaat tggttcaatc tggtgctgaa gtcaaaaagc caggtgcctc tgtcaaggtt       60

tcttgtaaag cttccggtta caccttcact aattactgga tgcactgggt tagacaagct      120

ccaggtcaag gtttggaatg gatgggtgag atcgacccat ttgattctta tactaattac      180

aatcaaaagt tcaagggtcg tgttactatg accatagaca catccactaa taccgtttac      240

atggaattat cttccttgag atccgaggac actgctgttt attattgcgc tcgtccaggt      300

tttacctacg gtggtatgga tttttggggt caaggtactt tggttaccgt ttcttct         357


<210>  154
<211>  119
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Amino acid sequence of the VH domain of the anti-hPD1 antibody designated #12

<400>  154

Gln Val Gln Leu Val Gln Ser Gly Ala Glu Val Lys Lys Pro Gly Ala 
1               5                   10                  15      


Ser Val Lys Val Ser Cys Lys Ala Ser Gly Tyr Thr Phe Thr Asn Tyr 
            20                  25                  30          


Trp Met His Trp Val Arg Gln Ala Pro Gly Gln Gly Leu Glu Trp Met 
        35                  40                  45              


Gly Glu Ile Asp Pro Phe Asp Ser Tyr Thr Asn Tyr Asn Gln Lys Phe 
    50                  55                  60                  


Lys Gly Arg Val Thr Met Thr Ile Asp Thr Ser Thr Asn Thr Val Tyr 
65                  70                  75                  80  


Met Glu Leu Ser Ser Leu Arg Ser Glu Asp Thr Ala Val Tyr Tyr Cys 
                85                  90                  95      


Ala Arg Pro Gly Phe Thr Tyr Gly Gly Met Asp Phe Trp Gly Gln Gly 
            100                 105                 110         


Thr Leu Val Thr Val Ser Ser 
        115                 


<210>  155
<211>  339
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Nucleotide sequence encoding the VL domain of the anti-hPD1 antibody designated #12

<400>  155
gatattgtta tgacccaatc cccagattcc ttggctgtct ctttgggtga aagagctact       60

attagttgta gatcttccca atctttgttg atttccggta accaaaagaa ctatttggcc      120

tggtatcaac aaaagccagg tcaaccacct aagttattga tctacggtgc ttccaccaga      180

gattctggtg ttccagatcg tttctctggt tctggttctg gtactgattt caccttaact      240

atttcttctt tgcaagccga agacgtcgct gtttactact gtcaaaacaa ccattattac      300

ccttacacct tcggtggtgg taccaaggtc gaaattaag                             339


<210>  156
<211>  113
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Amino acid sequence of the VL domain of the anti-hPD1 antibody designated #12

<400>  156

Asp Ile Val Met Thr Gln Ser Pro Asp Ser Leu Ala Val Ser Leu Gly 
1               5                   10                  15      


Glu Arg Ala Thr Ile Ser Cys Arg Ser Ser Gln Ser Leu Leu Ile Ser 
            20                  25                  30          


Gly Asn Gln Lys Asn Tyr Leu Ala Trp Tyr Gln Gln Lys Pro Gly Gln 
        35                  40                  45              


Pro Pro Lys Leu Leu Ile Tyr Gly Ala Ser Thr Arg Asp Ser Gly Val 
    50                  55                  60                  


Pro Asp Arg Phe Ser Gly Ser Gly Ser Gly Thr Asp Phe Thr Leu Thr 
65                  70                  75                  80  


Ile Ser Ser Leu Gln Ala Glu Asp Val Ala Val Tyr Tyr Cys Gln Asn 
                85                  90                  95      


Asn His Tyr Tyr Pro Tyr Thr Phe Gly Gly Gly Thr Lys Val Glu Ile 
            100                 105                 110         


Lys 
    


<210>  157
<211>  357
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Nucleotide sequence encoding the VH domain of the anti-hPD1 antibody designated #13

<400>  157
caagttcaat tggttcaatc tggtgctgaa gtcaaaaagc caggtgcctc tgtcaaggtt       60

tcttgtaaag cttccggtta caccttcact tcttactgga ttcactgggt tagacaagct      120

ccaggtcaag gtttggaatg gatgggtgag atcgacccat atgattctta tactaattac      180

aatcaaaagt tcaagggtcg tgttactatg accgtagaca aatccactaa taccgtttac      240

atggaattat cttccttaac atccgaggac actgctgttt attattgcgc tcgtccaggt      300

cttacctacg gtggtatgga tttttggggt caaggtactt tggttaccgt ttcttct         357


<210>  158
<211>  119
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Amino acid sequence of the VH domain of the anti-hPD1 antibody designated #13

<400>  158

Gln Val Gln Leu Val Gln Ser Gly Ala Glu Val Lys Lys Pro Gly Ala 
1               5                   10                  15      


Ser Val Lys Val Ser Cys Lys Ala Ser Gly Tyr Thr Phe Thr Ser Tyr 
            20                  25                  30          


Trp Ile His Trp Val Arg Gln Ala Pro Gly Gln Gly Leu Glu Trp Met 
        35                  40                  45              


Gly Glu Ile Asp Pro Tyr Asp Ser Tyr Thr Asn Tyr Asn Gln Lys Phe 
    50                  55                  60                  


Lys Gly Arg Val Thr Met Thr Val Asp Lys Ser Thr Asn Thr Val Tyr 
65                  70                  75                  80  


Met Glu Leu Ser Ser Leu Thr Ser Glu Asp Thr Ala Val Tyr Tyr Cys 
                85                  90                  95      


Ala Arg Pro Gly Leu Thr Tyr Gly Gly Met Asp Phe Trp Gly Gln Gly 
            100                 105                 110         


Thr Leu Val Thr Val Ser Ser 
        115                 


<210>  159
<211>  339
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  DNA sequence encoding the VL domain of the anti-hPD1 antibody designated #13

<400>  159
gatattcaaa tgacccaatc cccatcctct ttgtccgctt ctgttggtga cagagtcacc       60

atcacttgta agtcctctca atccttgttg aattctggta accaaaaaaa ctacttggcc      120

tggtaccaac aaaagccagg taagcctcct aaattgttga tttatggtgc ctccactaga      180

gattctggtg tcccagctaa attctctggt tctggttccg gtactgactt tactttgact      240

atttcttcct tgcaaccaga agacgtcgcc acctattact gtcaaaatga ccactactac      300

ccatacactt ttggtggtgg tactaaggtt gaaattaaa                             339


<210>  160
<211>  113
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Amino acid sequence of the VL domain of the anti-hPD1 antibody designated #13

<400>  160

Asp Ile Gln Met Thr Gln Ser Pro Ser Ser Leu Ser Ala Ser Val Gly 
1               5                   10                  15      


Asp Arg Val Thr Ile Thr Cys Lys Ser Ser Gln Ser Leu Leu Asn Ser 
            20                  25                  30          


Gly Asn Gln Lys Asn Tyr Leu Ala Trp Tyr Gln Gln Lys Pro Gly Lys 
        35                  40                  45              


Pro Pro Lys Leu Leu Ile Tyr Gly Ala Ser Thr Arg Asp Ser Gly Val 
    50                  55                  60                  


Pro Ala Lys Phe Ser Gly Ser Gly Ser Gly Thr Asp Phe Thr Leu Thr 
65                  70                  75                  80  


Ile Ser Ser Leu Gln Pro Glu Asp Val Ala Thr Tyr Tyr Cys Gln Asn 
                85                  90                  95      


Asp His Tyr Tyr Pro Tyr Thr Phe Gly Gly Gly Thr Lys Val Glu Ile 
            100                 105                 110         


Lys 
    


<210>  161
<211>  357
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Nucleotide sequence encoding the VH domain of the anti-hPD1 antibody designated #14

<400>  161
caagttcaat tggttcaatc tggtgctgaa gtcaaaaagc caggtgcctc tgtcaaggtt       60

tcttgtaaag cttccggtta caccttcact aattactgga ttcactgggt tagacaagct      120

ccaggtcaag gtttggaatg gatgggtgag atcgacccat atgattctta tactaattac      180

aatcaaaagt tcaagggtcg tgttactatg accggagaca aatccactag taccgtttac      240

atggaattat cttccttgac atccgaggac actgctgttt attattgcgc tcgtccaggt      300

tatacctacg gtggtatgga tttttggggt caaggtactt tggttaccgt ttcttct         357


<210>  162
<211>  119
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Amino acid sequence of the VH domain of the anti-hPD1 antibody designated #14

<400>  162

Gln Val Gln Leu Val Gln Ser Gly Ala Glu Val Lys Lys Pro Gly Ala 
1               5                   10                  15      


Ser Val Lys Val Ser Cys Lys Ala Ser Gly Tyr Thr Phe Thr Asn Tyr 
            20                  25                  30          


Trp Ile His Trp Val Arg Gln Ala Pro Gly Gln Gly Leu Glu Trp Met 
        35                  40                  45              


Gly Glu Ile Asp Pro Tyr Asp Ser Tyr Thr Asn Tyr Asn Gln Lys Phe 
    50                  55                  60                  


Lys Gly Arg Val Thr Met Thr Gly Asp Lys Ser Thr Ser Thr Val Tyr 
65                  70                  75                  80  


Met Glu Leu Ser Ser Leu Thr Ser Glu Asp Thr Ala Val Tyr Tyr Cys 
                85                  90                  95      


Ala Arg Pro Gly Tyr Thr Tyr Gly Gly Met Asp Phe Trp Gly Gln Gly 
            100                 105                 110         


Thr Leu Val Thr Val Ser Ser 
        115                 


<210>  163
<211>  339
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  DNA sequence encoding the VL domain of the anti-hPD1 antibody designated #14

<400>  163
gatattcaaa tgacccaatc cccatcctct ttgtccgctt ctgttggtga cagagtcacc       60

atcacttgta agtcctctca atccttgttt aattctggta accaaaaaaa ctacttggcc      120

tggtaccaac aaaagccagg taagcttcct aaattgttga tttatggtgc ctccactaga      180

gattctggtg tcccatctag attctctggt tctggttccg gtactgactt tactttgact      240

atttcttcct tgcaaccaga agacgtcgcc acctattact gtcaaaatga ccactactac      300

ccatacactt ttggtggtgg tactaaggtt gaaattaaa                             339


<210>  164
<211>  113
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Amino acid sequence of the VL domain of the anti-hPD1 antibody designated #14

<400>  164

Asp Ile Gln Met Thr Gln Ser Pro Ser Ser Leu Ser Ala Ser Val Gly 
1               5                   10                  15      


Asp Arg Val Thr Ile Thr Cys Lys Ser Ser Gln Ser Leu Phe Asn Ser 
            20                  25                  30          


Gly Asn Gln Lys Asn Tyr Leu Ala Trp Tyr Gln Gln Lys Pro Gly Lys 
        35                  40                  45              


Leu Pro Lys Leu Leu Ile Tyr Gly Ala Ser Thr Arg Asp Ser Gly Val 
    50                  55                  60                  


Pro Ser Arg Phe Ser Gly Ser Gly Ser Gly Thr Asp Phe Thr Leu Thr 
65                  70                  75                  80  


Ile Ser Ser Leu Gln Pro Glu Asp Val Ala Thr Tyr Tyr Cys Gln Asn 
                85                  90                  95      


Asp His Tyr Tyr Pro Tyr Thr Phe Gly Gly Gly Thr Lys Val Glu Ile 
            100                 105                 110         


Lys 
    


<210>  165
<211>  357
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  DNA sequence encoding the VH domain of the anti-hPD1 antibody designated #15

<400>  165
caagttcaat tggttcaatc tggtgctgaa gtcaaaaagc caggtgcctc tgtcaaggtt       60

tcttgtaaag cttccggtta caccttcact aattactgga tgcactgggt tagacaagct      120

ccaggtcaag gtttggaatg gatgggtgag atcgacccat atgattctta tactaattac      180

aatcaaaagt tcaagggtcg tgttactatg accagagaca catccactaa taccgtttac      240

atggaattat cttccttgag atccgaggac actgctgttt attattgcgc tcgtccaggt      300

cttacctacg gtggtatgga tttttggggt caaggtactt tggttaccgt ttcttct         357


<210>  166
<211>  119
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Amino acid sequence of the VH domain of the anti-hPD1 antibody designated #15

<400>  166

Gln Val Gln Leu Val Gln Ser Gly Ala Glu Val Lys Lys Pro Gly Ala 
1               5                   10                  15      


Ser Val Lys Val Ser Cys Lys Ala Ser Gly Tyr Thr Phe Thr Asn Tyr 
            20                  25                  30          


Trp Met His Trp Val Arg Gln Ala Pro Gly Gln Gly Leu Glu Trp Met 
        35                  40                  45              


Gly Glu Ile Asp Pro Tyr Asp Ser Tyr Thr Asn Tyr Asn Gln Lys Phe 
    50                  55                  60                  


Lys Gly Arg Val Thr Met Thr Arg Asp Thr Ser Thr Asn Thr Val Tyr 
65                  70                  75                  80  


Met Glu Leu Ser Ser Leu Arg Ser Glu Asp Thr Ala Val Tyr Tyr Cys 
                85                  90                  95      


Ala Arg Pro Gly Leu Thr Tyr Gly Gly Met Asp Phe Trp Gly Gln Gly 
            100                 105                 110         


Thr Leu Val Thr Val Ser Ser 
        115                 


<210>  167
<211>  339
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  DNA sequence encoding the VL domain of the anti-hPD1 antibody designated #15

<400>  167
gatattcaaa tgacccaatc cccatcctct ttgtccgctt ctgttggtga cagagtcacc       60

atcacttgta ggtcctctca atccttgttt aattctggta accaaaaaaa ctacttggcc      120

tggtaccaac aaaagccagg taagcctcct aaattgttga tttatggtgc ctccactaga      180

gaatctggtg tcccatatag attctctggt tctggttccg gtactgactt tactttgact      240

atttcttcct tgcaaccaga agacgtcgcc acctattact gtcaaaatga ccactactac      300

ccatacactt ttggtggtgg tactaaggtt gaaattaaa                             339


<210>  168
<211>  113
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Amino acid sequence of the VL domain of the anti-hPD1 antibody designated #15

<400>  168

Asp Ile Gln Met Thr Gln Ser Pro Ser Ser Leu Ser Ala Ser Val Gly 
1               5                   10                  15      


Asp Arg Val Thr Ile Thr Cys Arg Ser Ser Gln Ser Leu Phe Asn Ser 
            20                  25                  30          


Gly Asn Gln Lys Asn Tyr Leu Ala Trp Tyr Gln Gln Lys Pro Gly Lys 
        35                  40                  45              


Pro Pro Lys Leu Leu Ile Tyr Gly Ala Ser Thr Arg Glu Ser Gly Val 
    50                  55                  60                  


Pro Tyr Arg Phe Ser Gly Ser Gly Ser Gly Thr Asp Phe Thr Leu Thr 
65                  70                  75                  80  


Ile Ser Ser Leu Gln Pro Glu Asp Val Ala Thr Tyr Tyr Cys Gln Asn 
                85                  90                  95      


Asp His Tyr Tyr Pro Tyr Thr Phe Gly Gly Gly Thr Lys Val Glu Ile 
            100                 105                 110         


Lys 
    


<210>  169
<211>  5
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Consensus amino acid sequence for anti-hPD1 VH CDR1s


<220>
<221>  X
<222>  (1)..(1)
<223>  X is asparagine, glutamine, threonine, alanine, or serine

<220>
<221>  X
<222>  (4)..(4)
<223>  X is methionine, leucine, phenylalanine, valine, alanine, or 
       isoleucine

<400>  169

Xaa Tyr Trp Xaa His 
1               5   


<210>  170
<211>  5
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Second consensus amino acid sequence for anti-hPD1 VH CDR1s


<220>
<221>  X
<222>  (1)..(1)
<223>  X is asparagine or serine

<220>
<221>  X
<222>  (4)..(4)
<223>  X is methionine or isoleucine

<400>  170

Xaa Tyr Trp Xaa His 
1               5   


<210>  171
<211>  17
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Consensus amino acid sequence for anti-hPD1 VH CDR2s


<220>
<221>  X
<222>  (1)..(17)
<223>  X is tyrosine, tryptophan, phenylalanine, threonine, alanine, or 
       serine

<400>  171

Glu Ile Asp Pro Xaa Asp Ser Tyr Thr Asn Tyr Asn Gln Lys Phe Lys 
1               5                   10                  15      


Gly 
    


<210>  172
<211>  17
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Second consensus amino acid sequence for anti-hPD1 VH CDR2s


<220>
<221>  X
<222>  (1)..(17)
<223>  X is tyrosine, phenylalanine, or serine

<400>  172

Glu Ile Asp Pro Xaa Asp Ser Tyr Thr Asn Tyr Asn Gln Lys Phe Lys 
1               5                   10                  15      


Gly 
    


<210>  173
<211>  10
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Consensus amino acid sequence for anti-hPD1 VH CDR3s


<220>
<221>  X
<222>  (3)..(3)
<223>  X is phenylalanine, tyrosine, leucine, isoleucine, valine, methionine, alanine, asparagine, or glutamine

<220>
<221>  X
<222>  (10)..(10)
<223>  X is phenylalanine, or tyrosine

<400>  173

Pro Gly Xaa Thr Tyr Gly Gly Met Asp Xaa 
1               5                   10  


<210>  174
<211>  10
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Second consensus amino acid sequence for anti-hPD1 VH CDR3s


<220>
<221>  X
<222>  (3)..(3)
<223>  X is phenylalanine, tyrosine, leucine, or asparagine

<220>
<221>  X
<222>  (10)..(10)
<223>  X is phenylalanine or tyrosine

<400>  174

Pro Gly Xaa Thr Tyr Gly Gly Met Asp Xaa 
1               5                   10  


<210>  175
<211>  17
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Consensus amino acid sequence for anti-hPD1 VL CDR1s


<220>
<221>  X
<222>  (1)..(1)
<223>  X is arginine, glutamine, asparagine, or lysine

<220>
<221>  X
<222>  (7)..(7)
<223>  X is phenylalanine, valine, isoleucine, alanine, tyrosine, 
       methionine, or leucine

<220>
<221>  X
<222>  (8)..(8)
<223>  X is asparagine, glutamine, leucine, valine, methionine, alanine, phenylalanine, or isoleucine

<400>  175

Xaa Ser Ser Gln Ser Leu Xaa Xaa Ser Gly Asn Gln Lys Asn Tyr Leu 
1               5                   10                  15      


Ala 
    


<210>  176
<211>  17
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Second consensus amino acid sequence for anti-hPD1 VL CDR1s


<220>
<221>  X
<222>  (1)..(1)
<223>  X is arginine or lysine

<220>
<221>  X
<222>  (7)..(7)
<223>  X is phenylalanine or leucine

<220>
<221>  X
<222>  (8)..(8)
<223>  X is asparagine or isoleucine

<400>  176

Xaa Ser Ser Gln Ser Leu Xaa Xaa Ser Gly Asn Gln Lys Asn Tyr Leu 
1               5                   10                  15      


Ala 
    


<210>  177
<211>  7
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Consensus amino acid sequence for anti-hPD1 VL CDR2s


<220>
<221>  X
<222>  (1)..(7)
<223>  X is aspartate or glutamate

<400>  177

Gly Ala Ser Thr Arg Xaa Ser 
1               5           


<210>  178
<211>  9
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Consensus amino acid sequence for anti-hPD1 VL CDR3s


<220>
<221>  X
<222>  (1)..(9)
<223>  X is aspartate, glutamate, glutamine, or asparagine

<400>  178

Gln Asn Xaa His Tyr Tyr Pro Tyr Thr 
1               5                   


<210>  179
<211>  9
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Second consensus amino acid sequence for anti-hPD1 VL CDR3s


<220>
<221>  X
<222>  (1)..(9)
<223>  X is aspartate or asparagine

<400>  179

Gln Asn Xaa His Tyr Tyr Pro Tyr Thr 
1               5                   


<210>  180
<211>  119
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Consensus amino acid sequence for anti-hPD1 VH domains


<220>
<221>  X
<222>  (26)..(26)
<223>  X is glycine, alanine, threonine, or serine

<220>
<221>  X
<222>  (31)..(31)
<223>  X is asparagine, glutamine, threonine, alanine, or serine

<220>
<221>  X
<222>  (34)..(34)
<223>  X is isoleucine, leucine, valine, alanine, phenylalanine, or 
       methionine

<220>
<221>  X
<222>  (54)..(54)
<223>  X is tyrosine, tryptophan, threonine, phenylalanine, leucine, 
       valine, isoleucine, alanine, threonine, or serine

<220>
<221>  X
<222>  (72)..(72)
<223>  X is valine, methionine, leucine, phenylalanine, alanine, isoleucine, valine, arginine, lysine, glutamine, asparagine, or glycine

<220>
<221>  X
<222>  (74)..(74)
<223>  X is lysine, arginine, glutamine, asparagine, serine, or 
       threonine

<220>
<221>  X
<222>  (77)..(77)
<223>  X is asparagine, glutamine, threonine, alanine, or serine

<220>
<221>  X
<222>  (87)..(87)
<223>  X is arginine, lysine, glutamine, asparagine, serine, threonine, alanine, proline, or glycine

<220>
<221>  X
<222>  (101)..(101)
<223>  X is phenylalanine, valine, isoleucine, alanine, asparagine, glutamine, tyrosine, tryptophan, threonine, serine, methionine, or leucine

<220>
<221>  X
<222>  (108)..(108)
<223>  X is phenylalanine, leucine, valine, isoleucine, alanine, 
       tryptophan, threonine, serine, or tyrosine

<400>  180

Gln Val Gln Leu Val Gln Ser Gly Ala Glu Val Lys Lys Pro Gly Ala 
1               5                   10                  15      


Ser Val Lys Val Ser Cys Lys Ala Ser Xaa Tyr Thr Phe Thr Xaa Tyr 
            20                  25                  30          


Trp Xaa His Trp Val Arg Gln Ala Pro Gly Gln Gly Leu Glu Trp Met 
        35                  40                  45              


Gly Glu Ile Asp Pro Xaa Asp Ser Tyr Thr Asn Tyr Asn Gln Lys Phe 
    50                  55                  60                  


Lys Gly Arg Val Thr Met Thr Xaa Asp Xaa Ser Thr Xaa Thr Val Tyr 
65                  70                  75                  80  


Met Glu Leu Ser Ser Leu Xaa Ser Glu Asp Thr Ala Val Tyr Tyr Cys 
                85                  90                  95      


Ala Arg Pro Gly Xaa Thr Tyr Gly Gly Met Asp Xaa Trp Gly Gln Gly 
            100                 105                 110         


Thr Leu Val Thr Val Ser Ser 
        115                 


<210>  181
<211>  119
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Second consensus amino acid sequence for anti-hPD1 VH domains


<220>
<221>  X
<222>  (26)..(26)
<223>  X is glycine or serine

<220>
<221>  X
<222>  (31)..(31)
<223>  X is asparagine or serine

<220>
<221>  X
<222>  (34)..(34)
<223>  X is isoleucine or methionine

<220>
<221>  X
<222>  (54)..(54)
<223>  X is tyrosine, phenylalanine, or serine

<220>
<221>  X
<222>  (72)..(72)
<223>  X is valine, isoleucine, arginine, or glycine

<220>
<221>  X
<222>  (74)..(74)
<223>  X is lysine or threonine

<220>
<221>  X
<222>  (77)..(77)
<223>  X is asparagine or serine

<220>
<221>  X
<222>  (87)..(87)
<223>  X is arginine, threonine, or glycine

<220>
<221>  X
<222>  (101)..(101)
<223>  X is phenylalanine, asparagine, tyrosine, or leucine

<220>
<221>  X
<222>  (108)..(108)
<223>  X is phenylalanine or tyrosine

<400>  181

Gln Val Gln Leu Val Gln Ser Gly Ala Glu Val Lys Lys Pro Gly Ala 
1               5                   10                  15      


Ser Val Lys Val Ser Cys Lys Ala Ser Xaa Tyr Thr Phe Thr Xaa Tyr 
            20                  25                  30          


Trp Xaa His Trp Val Arg Gln Ala Pro Gly Gln Gly Leu Glu Trp Met 
        35                  40                  45              


Gly Glu Ile Asp Pro Xaa Asp Ser Tyr Thr Asn Tyr Asn Gln Lys Phe 
    50                  55                  60                  


Lys Gly Arg Val Thr Met Thr Xaa Asp Xaa Ser Thr Xaa Thr Val Tyr 
65                  70                  75                  80  


Met Glu Leu Ser Ser Leu Xaa Ser Glu Asp Thr Ala Val Tyr Tyr Cys 
                85                  90                  95      


Ala Arg Pro Gly Xaa Thr Tyr Gly Gly Met Asp Xaa Trp Gly Gln Gly 
            100                 105                 110         


Thr Leu Val Thr Val Ser Ser 
        115                 


<210>  182
<211>  113
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Consensus amino acid sequence for anti-hPD1 VL domains


<220>
<221>  X
<222>  (3)..(3)
<223>  X is valine, isoleucine, methionine, leucine, phenylalanine, 
       alanine, asparagine, or glutamine

<220>
<221>  X
<222>  (9)..(9)
<223>  X is serine, threonine, alanine, glutamate, or aspartate

<220>
<221>  X
<222>  (12)..(12)
<223>  X is serine, threonine, valine, leucine, isoleucine, or alanine

<220>
<221>  X
<222>  (13)..(13)
<223>  X is alanine, leucine, isoleucine, methionine, phenylalanine, or 
       valine

<220>
<221>  X
<222>  (15)..(15)
<223>  X is valine, isoleucine, methionine, phenylalanine, alanine, or 
       leucine

<220>
<221>  X
<222>  (17)..(17)
<223>  X is aspartate or glutamate

<220>
<221>  X
<222>  (19)..(19)
<223>  X is valine, isoleucine, methionine, leucine, phenylalanine or 
       alanine

<220>
<221>  X
<222>  (22)..(22)
<223>  X is threonine, asparagine, glutamine, alanine, or serine

<220>
<221>  X
<222>  (24)..(24)
<223>  X is lysine, glutamine, asparagine, or arginine

<220>
<221>  X
<222>  (30)..(30)
<223>  X is phenylalanine, valine, isoleucine, alanine, tyrosine, 
       methionine, or leucine

<220>
<221>  X
<222>  (31)..(31)
<223>  X is asparagine, glutamine, leucine, valine, methionine, alanine,
       phenylalanine, or isoleucine

<220>
<221>  X
<222>  (48)..(48)
<223>  X is lysine, arginine, asparagine, or glutamine

<220>
<221>  X
<222>  (49)..(49)
<223>  X is valine, isoleucine, methionine, phenylalanine, alanine, 
       proline, or leucine

<220>
<221>  X
<222>  (61)..(61)
<223>  X is aspartate or glutamate

<220>
<221>  X
<222>  (66)..(66)
<223>  X is tyrosine, tryptophan, phenylalanine, threonine, alanine, 
       valine, leucine, isoleucine, serine, glutamate, or aspartate

<220>
<221>  X
<222>  (67)..(67)
<223>  X is arginine, lysine, asparagine, or glutatmine

<220>
<221>  X
<222>  (86)..(86)
<223>  X is proline, valine, leucine, isoleucine, or alanine

<220>
<221>  X
<222>  (91)..(91)
<223>  X is threonine, serine, isoleucine, methionine, leucine, 
       phenylalanine, alanine, or valine

<220>
<221>  X
<222>  (97)..(97)
<223>  X is aspartate, glutamate, glutamine, or asparagine

<400>  182

Asp Ile Xaa Met Thr Gln Ser Pro Xaa Ser Leu Xaa Xaa Ser Xaa Gly 
1               5                   10                  15      


Xaa Arg Xaa Thr Ile Xaa Cys Xaa Ser Ser Gln Ser Leu Xaa Xaa Ser 
            20                  25                  30          


Gly Asn Gln Lys Asn Tyr Leu Ala Trp Tyr Gln Gln Lys Pro Gly Xaa 
        35                  40                  45              


Xaa Pro Lys Leu Leu Ile Tyr Gly Ala Ser Thr Arg Xaa Ser Gly Val 
    50                  55                  60                  


Pro Xaa Xaa Phe Ser Gly Ser Gly Ser Gly Thr Asp Phe Thr Leu Thr 
65                  70                  75                  80  


Ile Ser Ser Leu Gln Xaa Glu Asp Val Ala Xaa Tyr Tyr Cys Gln Asn 
                85                  90                  95      


Xaa His Tyr Tyr Pro Tyr Thr Phe Gly Gly Gly Thr Lys Val Glu Ile 
            100                 105                 110         


Lys 
    


<210>  183
<211>  113
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Second consensus amino acid sequence for anti-hPD1 VL domains


<220>
<221>  X
<222>  (3)..(3)
<223>  X is valine or glutamine

<220>
<221>  X
<222>  (9)..(9)
<223>  X is serine or aspartate

<220>
<221>  X
<222>  (12)..(12)
<223>  X is serine or alanine

<220>
<221>  X
<222>  (13)..(13)
<223>  X is alanine or valine

<220>
<221>  X
<222>  (15)..(15)
<223>  X is valine or leucine

<220>
<221>  X
<222>  (17)..(17)
<223>  X is aspartate or glutamate

<220>
<221>  X
<222>  (19)..(19)
<223>  X is valine or alanine

<220>
<221>  X
<222>  (22)..(22)
<223>  X is threonine, asparagine, or serine

<220>
<221>  X
<222>  (24)..(24)
<223>  X is lysine or arginine

<220>
<221>  X
<222>  (30)..(30)
<223>  X is phenylalanine or leucine

<220>
<221>  X
<222>  (31)..(31)
<223>  X is asparagine or isoleucine

<220>
<221>  X
<222>  (48)..(48)
<223>  X is lysine or glutamine

<220>
<221>  X
<222>  (49)..(49)
<223>  X is valine, proline, or leucine

<220>
<221>  X
<222>  (61)..(61)
<223>  X is aspartate or glutamate

<220>
<221>  X
<222>  (66)..(66)
<223>  X is tyrosine, alanine, serine, or aspartate

<220>
<221>  X
<222>  (67)..(67)
<223>  X is arginine or lysine

<220>
<221>  X
<222>  (86)..(86)
<223>  X is proline or alanine

<220>
<221>  X
<222>  (91)..(91)
<223>  X is threonine or valine

<220>
<221>  X
<222>  (97)..(97)
<223>  X is aspartate or asparagine

<400>  183

Asp Ile Xaa Met Thr Gln Ser Pro Xaa Ser Leu Xaa Xaa Ser Xaa Gly 
1               5                   10                  15      


Xaa Arg Xaa Thr Ile Xaa Cys Xaa Ser Ser Gln Ser Leu Xaa Xaa Ser 
            20                  25                  30          


Gly Asn Gln Lys Asn Tyr Leu Ala Trp Tyr Gln Gln Lys Pro Gly Xaa 
        35                  40                  45              


Xaa Pro Lys Leu Leu Ile Tyr Gly Ala Ser Thr Arg Xaa Ser Gly Val 
    50                  55                  60                  


Pro Xaa Xaa Phe Ser Gly Ser Gly Ser Gly Thr Asp Phe Thr Leu Thr 
65                  70                  75                  80  


Ile Ser Ser Leu Gln Xaa Glu Asp Val Ala Xaa Tyr Tyr Cys Gln Asn 
                85                  90                  95      


Xaa His Tyr Tyr Pro Tyr Thr Phe Gly Gly Gly Thr Lys Val Glu Ile 
            100                 105                 110         


Lys 
    


<210>  184
<211>  1338
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  DNA sequence encoding the human IgG4 HC of an altered anti-PD1 antibody #1 with the alterations G44D, Q105R, K147R, H168R, and V173C

<400>  184
caggtgcagc tggtgcagtc tggcgccgaa gtgaagaaac ctggcgcctc cgtgaaggtg       60

tcctgcaagg cttccggcta cacctttacc aactactgga tccactgggt gcgacaggcc      120

ccaggccagg acctggaatg gatgggcgag atcgacccct acgactccta caccaactac      180

aaccagaaat tcaagggccg cgtgaccatg accgtggaca agtccacctc caccgtgtac      240

atggaactgt cctccctgcg gagcgaggac accgccgtgt actactgtgc cagacccggc      300

ttcacctacg gcggcatgga tttctggggc agaggcaccc tcgtgaccgt gtcctctgct      360

tccaccaagg gcccctccgt gtttcctctg gccccttgct ccagatccac ctccgagtct      420

accgccgctc tgggatgtct cgtgcgggac tacttccccg agcccgtgac agtgtcttgg      480

aactctggcg ccctgacctc tggcgtgcgg acctttcctg cttgcctgca gtctagcggc      540

ctgtactccc tgtcctccgt cgtgactgtg ccctccagct ctctgggcac caagacctac      600

acctgtaacg tggaccacaa gccctccaac accaaggtgg acaagcgggt ggaatctaag      660

tacggccctc cctgccctcc ttgcccagcc cctgagtttc tgggcggacc cagcgtgttc      720

ctgttccccc caaagcccaa ggacaccctg atgatctccc ggacccccga agtgacctgc      780

gtggtggtgg atgtgtccca ggaagatccc gaggtgcagt tcaattggta cgtggacggc      840

gtggaagtgc acaacgccaa gaccaagcct agagaggaac agttcaactc cacctaccgg      900

gtggtgtccg tgctgaccgt gctgcaccag gattggctga acggcaaaga gtacaagtgc      960

aaggtgtcca acaagggcct gcccagctcc atcgaaaaga ccatctccaa ggccaagggc     1020

cagccccggg aaccccaggt gtacacactg cctccaagcc aggaagagat gaccaagaac     1080

caggtgtccc tgacctgtct cgtgaaaggc ttctacccct ccgacatcgc cgtggaatgg     1140

gagtccaacg gccagcctga gaacaactac aagaccaccc cccctgtgct ggactccgac     1200

ggctccttct tcctgtactc tcggctgaca gtggataagt cccggtggca ggaaggcaac     1260

gtgttctcct gctccgtgat gcacgaggcc ctgcacaacc actacaccca gaagtccctg     1320

tccctgtctc tgggaaag                                                   1338


<210>  185
<211>  446
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Amino acid sequence of the human IgG4 HC of an altered anti-PD1 antibody #1 with the alterations G44D, Q105R, K147R, H168R, and V173C

<400>  185

Gln Val Gln Leu Val Gln Ser Gly Ala Glu Val Lys Lys Pro Gly Ala 
1               5                   10                  15      


Ser Val Lys Val Ser Cys Lys Ala Ser Gly Tyr Thr Phe Thr Asn Tyr 
            20                  25                  30          


Trp Ile His Trp Val Arg Gln Ala Pro Gly Gln Asp Leu Glu Trp Met 
        35                  40                  45              


Gly Glu Ile Asp Pro Tyr Asp Ser Tyr Thr Asn Tyr Asn Gln Lys Phe 
    50                  55                  60                  


Lys Gly Arg Val Thr Met Thr Val Asp Lys Ser Thr Ser Thr Val Tyr 
65                  70                  75                  80  


Met Glu Leu Ser Ser Leu Arg Ser Glu Asp Thr Ala Val Tyr Tyr Cys 
                85                  90                  95      


Ala Arg Pro Gly Phe Thr Tyr Gly Gly Met Asp Phe Trp Gly Arg Gly 
            100                 105                 110         


Thr Leu Val Thr Val Ser Ser Ala Ser Thr Lys Gly Pro Ser Val Phe 
        115                 120                 125             


Pro Leu Ala Pro Cys Ser Arg Ser Thr Ser Glu Ser Thr Ala Ala Leu 
    130                 135                 140                 


Gly Cys Leu Val Arg Asp Tyr Phe Pro Glu Pro Val Thr Val Ser Trp 
145                 150                 155                 160 


Asn Ser Gly Ala Leu Thr Ser Gly Val Arg Thr Phe Pro Ala Cys Leu 
                165                 170                 175     


Gln Ser Ser Gly Leu Tyr Ser Leu Ser Ser Val Val Thr Val Pro Ser 
            180                 185                 190         


Ser Ser Leu Gly Thr Lys Thr Tyr Thr Cys Asn Val Asp His Lys Pro 
        195                 200                 205             


Ser Asn Thr Lys Val Asp Lys Arg Val Glu Ser Lys Tyr Gly Pro Pro 
    210                 215                 220                 


Cys Pro Pro Cys Pro Ala Pro Glu Phe Leu Gly Gly Pro Ser Val Phe 
225                 230                 235                 240 


Leu Phe Pro Pro Lys Pro Lys Asp Thr Leu Met Ile Ser Arg Thr Pro 
                245                 250                 255     


Glu Val Thr Cys Val Val Val Asp Val Ser Gln Glu Asp Pro Glu Val 
            260                 265                 270         


Gln Phe Asn Trp Tyr Val Asp Gly Val Glu Val His Asn Ala Lys Thr 
        275                 280                 285             


Lys Pro Arg Glu Glu Gln Phe Asn Ser Thr Tyr Arg Val Val Ser Val 
    290                 295                 300                 


Leu Thr Val Leu His Gln Asp Trp Leu Asn Gly Lys Glu Tyr Lys Cys 
305                 310                 315                 320 


Lys Val Ser Asn Lys Gly Leu Pro Ser Ser Ile Glu Lys Thr Ile Ser 
                325                 330                 335     


Lys Ala Lys Gly Gln Pro Arg Glu Pro Gln Val Tyr Thr Leu Pro Pro 
            340                 345                 350         


Ser Gln Glu Glu Met Thr Lys Asn Gln Val Ser Leu Thr Cys Leu Val 
        355                 360                 365             


Lys Gly Phe Tyr Pro Ser Asp Ile Ala Val Glu Trp Glu Ser Asn Gly 
    370                 375                 380                 


Gln Pro Glu Asn Asn Tyr Lys Thr Thr Pro Pro Val Leu Asp Ser Asp 
385                 390                 395                 400 


Gly Ser Phe Phe Leu Tyr Ser Arg Leu Thr Val Asp Lys Ser Arg Trp 
                405                 410                 415     


Gln Glu Gly Asn Val Phe Ser Cys Ser Val Met His Glu Ala Leu His 
            420                 425                 430         


Asn His Tyr Thr Gln Lys Ser Leu Ser Leu Ser Leu Gly Lys 
        435                 440                 445     


<210>  186
<211>  660
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  DNA sequence encoding the human LC of an altered anti-PD1 antibody #1 with the alterations V43D, G100R, S131D, S162C, and  S174D

<400>  186
gacatccaga tgacccagtc cccctccagc ctgtctgcct ctgtgggcga cagagtgacc       60

atcacatgca agtcctccca gtccctgttc aactccggca accagaagaa ctacctggcc      120

tggtatcagc agaagcccgg caaggacccc aagctgctga tctacggcgc ctccaccaga      180

gactctggcg tgccctacag attctccggc tctggctctg gcaccgactt taccctgacc      240

atcagctccc tgcagcccga ggatgtggcc acctactact gccagaacga ccactactac      300

ccctacacct tcggcagagg caccaaggtg gaaatcaagc ggaccgtggc cgctccctcc      360

gtgttcatct tcccaccttc cgacgagcag ctgaagtccg gcaccgctga tgtcgtgtgc      420

ctgctgaaca acttctaccc ccgcgaggcc aaggtgcagt ggaaggtgga caacgccctg      480

cagtccggca actcccagga atgcgtgacc gagcaggact ccaaggacag cacctacgac      540

ctgtcctcca ccctgaccct gtccaaggcc gactacgaga agcacaaggt gtacgcctgc      600

gaagtgaccc accagggcct gtctagcccc gtgaccaagt ctttcaaccg gggcgagtgc      660


<210>  187
<211>  220
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Amino acid sequence  of the human LC of an altered anti-PD1 antibody #1 with the alterations V43D, G100R, S131D, S162C, and  S174D

<400>  187

Asp Ile Gln Met Thr Gln Ser Pro Ser Ser Leu Ser Ala Ser Val Gly 
1               5                   10                  15      


Asp Arg Val Thr Ile Thr Cys Lys Ser Ser Gln Ser Leu Phe Asn Ser 
            20                  25                  30          


Gly Asn Gln Lys Asn Tyr Leu Ala Trp Tyr Gln Gln Lys Pro Gly Lys 
        35                  40                  45              


Asp Pro Lys Leu Leu Ile Tyr Gly Ala Ser Thr Arg Asp Ser Gly Val 
    50                  55                  60                  


Pro Tyr Arg Phe Ser Gly Ser Gly Ser Gly Thr Asp Phe Thr Leu Thr 
65                  70                  75                  80  


Ile Ser Ser Leu Gln Pro Glu Asp Val Ala Thr Tyr Tyr Cys Gln Asn 
                85                  90                  95      


Asp His Tyr Tyr Pro Tyr Thr Phe Gly Arg Gly Thr Lys Val Glu Ile 
            100                 105                 110         


Lys Arg Thr Val Ala Ala Pro Ser Val Phe Ile Phe Pro Pro Ser Asp 
        115                 120                 125             


Glu Gln Leu Lys Ser Gly Thr Ala Asp Val Val Cys Leu Leu Asn Asn 
    130                 135                 140                 


Phe Tyr Pro Arg Glu Ala Lys Val Gln Trp Lys Val Asp Asn Ala Leu 
145                 150                 155                 160 


Gln Ser Gly Asn Ser Gln Glu Cys Val Thr Glu Gln Asp Ser Lys Asp 
                165                 170                 175     


Ser Thr Tyr Asp Leu Ser Ser Thr Leu Thr Leu Ser Lys Ala Asp Tyr 
            180                 185                 190         


Glu Lys His Lys Val Tyr Ala Cys Glu Val Thr His Gln Gly Leu Ser 
        195                 200                 205             


Ser Pro Val Thr Lys Ser Phe Asn Arg Gly Glu Cys 
    210                 215                 220 


<210>  188
<211>  1344
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  DNA sequence encoding the human IgG1 HC of an altered anti-CTLA4 antibody 10D4 with the alterations G44R, Q105D, K147D, H168D, V173C, R255K, D399R, and K409E

<400>  188
caagttcagc ttgttgagtc tgggggtgga gtagttgaac ctggtagaag cctccgattg       60

agttgcactg ctagtggatt cactttcagt tcatacggga tgcactgggt acgccaagca      120

ccaggcaagc gtctcgaatg ggtggccgtt atctggtatc acccctccaa aaaagactac      180

gccgacagcg ccaagggacg gtttaccatt agccgagata acagtaaaaa cactctctat      240

cttcaaatga acagccttcg cgctgaagac acagccgtct actattgtgc tagagctggc      300

cttcttggct acttcgacta ctggggagat ggcactctcg tcacagtgag ttctgctagc      360

accaagggcc catcggtctt ccccctggca ccctcctcca agagcacctc tgggggcaca      420

gcggccctgg gctgcctggt cgacgactac ttccccgaac cggtgacggt gtcgtggaac      480

tcaggcgccc tgaccagcgg cgtggacacc ttcccggctt gcctacagtc ctcaggactc      540

tactccctca gcagcgtggt gaccgtgccc tccagcagct tgggcaccca gacctacatc      600

tgcaacgtga atcacaagcc cagcaacacc aaggtggaca agaaagttga gcccaaatct      660

tgtgacaaaa ctcacacatg cccaccgtgc ccagcacctg aactcctggg gggaccgtca      720

gtcttcctct tccccccaaa acccaaggac accctcatga tctccaagac ccctgaggtc      780

acatgcgtgg tggtggacgt gagccacgaa gaccctgagg tcaagttcaa ctggtacgtg      840

gacggcgtgg aggtgcataa tgccaagaca aagccgcggg aggagcagta caacagcacg      900

taccgtgtgg tcagcgtcct caccgtcctg caccaggact ggctgaatgg caaggagtac      960

aagtgcaagg tctccaacaa agccctccca gcccccatcg agaaaaccat ctccaaagcc     1020

aaagggcagc cccgagaacc acaggtgtac accctgcccc catcccggga ggagatgacc     1080

aagaaccagg tcagcctgac ctgcctggtc aaaggcttct atcccagcga catcgccgtg     1140

gagtgggaga gcaatgggca gccggagaac aactacaaga ccacgcctcc cgtgctgcgg     1200

tccgacggct ccttcttcct ctatagcgag ctcaccgtgg acaagagcag gtggcagcag     1260

gggaacgtct tctcatgctc cgtgatgcat gaggctctgc acaaccacta cacgcagaag     1320

agcctctccc tgtctccggg taaa                                            1344


<210>  189
<211>  448
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Amino acid sequence of the human IgG1 HC of an altered anti-CTLA4 antibody 10D4 with the alterations G44R, Q105D, K147D, H168D, V173C, R255K, D399R, and K409E

<400>  189

Gln Val Gln Leu Val Glu Ser Gly Gly Gly Val Val Glu Pro Gly Arg 
1               5                   10                  15      


Ser Leu Arg Leu Ser Cys Thr Ala Ser Gly Phe Thr Phe Ser Ser Tyr 
            20                  25                  30          


Gly Met His Trp Val Arg Gln Ala Pro Gly Lys Arg Leu Glu Trp Val 
        35                  40                  45              


Ala Val Ile Trp Tyr His Pro Ser Lys Lys Asp Tyr Ala Asp Ser Ala 
    50                  55                  60                  


Lys Gly Arg Phe Thr Ile Ser Arg Asp Asn Ser Lys Asn Thr Leu Tyr 
65                  70                  75                  80  


Leu Gln Met Asn Ser Leu Arg Ala Glu Asp Thr Ala Val Tyr Tyr Cys 
                85                  90                  95      


Ala Arg Ala Gly Leu Leu Gly Tyr Phe Asp Tyr Trp Gly Asp Gly Thr 
            100                 105                 110         


Leu Val Thr Val Ser Ser Ala Ser Thr Lys Gly Pro Ser Val Phe Pro 
        115                 120                 125             


Leu Ala Pro Ser Ser Lys Ser Thr Ser Gly Gly Thr Ala Ala Leu Gly 
    130                 135                 140                 


Cys Leu Val Asp Asp Tyr Phe Pro Glu Pro Val Thr Val Ser Trp Asn 
145                 150                 155                 160 


Ser Gly Ala Leu Thr Ser Gly Val Asp Thr Phe Pro Ala Cys Leu Gln 
                165                 170                 175     


Ser Ser Gly Leu Tyr Ser Leu Ser Ser Val Val Thr Val Pro Ser Ser 
            180                 185                 190         


Ser Leu Gly Thr Gln Thr Tyr Ile Cys Asn Val Asn His Lys Pro Ser 
        195                 200                 205             


Asn Thr Lys Val Asp Lys Lys Val Glu Pro Lys Ser Cys Asp Lys Thr 
    210                 215                 220                 


His Thr Cys Pro Pro Cys Pro Ala Pro Glu Leu Leu Gly Gly Pro Ser 
225                 230                 235                 240 


Val Phe Leu Phe Pro Pro Lys Pro Lys Asp Thr Leu Met Ile Ser Lys 
                245                 250                 255     


Thr Pro Glu Val Thr Cys Val Val Val Asp Val Ser His Glu Asp Pro 
            260                 265                 270         


Glu Val Lys Phe Asn Trp Tyr Val Asp Gly Val Glu Val His Asn Ala 
        275                 280                 285             


Lys Thr Lys Pro Arg Glu Glu Gln Tyr Asn Ser Thr Tyr Arg Val Val 
    290                 295                 300                 


Ser Val Leu Thr Val Leu His Gln Asp Trp Leu Asn Gly Lys Glu Tyr 
305                 310                 315                 320 


Lys Cys Lys Val Ser Asn Lys Ala Leu Pro Ala Pro Ile Glu Lys Thr 
                325                 330                 335     


Ile Ser Lys Ala Lys Gly Gln Pro Arg Glu Pro Gln Val Tyr Thr Leu 
            340                 345                 350         


Pro Pro Ser Arg Glu Glu Met Thr Lys Asn Gln Val Ser Leu Thr Cys 
        355                 360                 365             


Leu Val Lys Gly Phe Tyr Pro Ser Asp Ile Ala Val Glu Trp Glu Ser 
    370                 375                 380                 


Asn Gly Gln Pro Glu Asn Asn Tyr Lys Thr Thr Pro Pro Val Leu Arg 
385                 390                 395                 400 


Ser Asp Gly Ser Phe Phe Leu Tyr Ser Glu Leu Thr Val Asp Lys Ser 
                405                 410                 415     


Arg Trp Gln Gln Gly Asn Val Phe Ser Cys Ser Val Met His Glu Ala 
            420                 425                 430         


Leu His Asn His Tyr Thr Gln Lys Ser Leu Ser Leu Ser Pro Gly Lys 
        435                 440                 445             


<210>  190
<211>  642
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  DNA sequence encoding the human LC of an altered anti-CTLA4 antibody 10D4 with the alterations V43R, G100D, S131R, Q160C, and S174R

<400>  190
gaaatcgtct tgacccaatc acctgggacc ctctccctgt cacctggaga gagagcaact       60

ttgagttgta gagctagtca gagtgtaacc tcatacctgg cttggtatca gcagaagcct      120

gggcaaaggc cacgccctct catatatggt gtaagtagtc gtgctactgg catccccgac      180

cgattcagcg gctccggttc tggtacagat ttcaccctta caatcagtag gctggaacca      240

gaggactttg cagtctacta ttgccaacag tatggtcgtt accctttcac attcggggat      300

gggacaaagg tggatattaa acgtacggtg gctgcaccat ctgtcttcat cttcccgcca      360

tctgatgagc agttgaaatc tggaactgcc cgcgttgtgt gcctgctgaa taacttctat      420

cccagagagg ccaaagtaca gtggaaggtg gataacgccc tccaatcggg taactcctgc      480

gagagtgtca cagagcagga cagcaaggac agcacctaca ggctcagcag caccctgacg      540

ctgagcaaag cagactacga gaaacacaaa gtctacgcct gcgaagtcac ccatcagggc      600

ctgagctcgc ccgtcacaaa gagcttcaac aggggagagt gt                         642


<210>  191
<211>  214
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Amino acid sequence of the human LC of an altered anti-CTLA4 antibody 10D4 with the alterations V43R, G100D, S131R, Q160C, and S174R

<400>  191

Glu Ile Val Leu Thr Gln Ser Pro Gly Thr Leu Ser Leu Ser Pro Gly 
1               5                   10                  15      


Glu Arg Ala Thr Leu Ser Cys Arg Ala Ser Gln Ser Val Thr Ser Tyr 
            20                  25                  30          


Leu Ala Trp Tyr Gln Gln Lys Pro Gly Gln Arg Pro Arg Pro Leu Ile 
        35                  40                  45              


Tyr Gly Val Ser Ser Arg Ala Thr Gly Ile Pro Asp Arg Phe Ser Gly 
    50                  55                  60                  


Ser Gly Ser Gly Thr Asp Phe Thr Leu Thr Ile Ser Arg Leu Glu Pro 
65                  70                  75                  80  


Glu Asp Phe Ala Val Tyr Tyr Cys Gln Gln Tyr Gly Arg Tyr Pro Phe 
                85                  90                  95      


Thr Phe Gly Asp Gly Thr Lys Val Asp Ile Lys Arg Thr Val Ala Ala 
            100                 105                 110         


Pro Ser Val Phe Ile Phe Pro Pro Ser Asp Glu Gln Leu Lys Ser Gly 
        115                 120                 125             


Thr Ala Arg Val Val Cys Leu Leu Asn Asn Phe Tyr Pro Arg Glu Ala 
    130                 135                 140                 


Lys Val Gln Trp Lys Val Asp Asn Ala Leu Gln Ser Gly Asn Ser Cys 
145                 150                 155                 160 


Glu Ser Val Thr Glu Gln Asp Ser Lys Asp Ser Thr Tyr Arg Leu Ser 
                165                 170                 175     


Ser Thr Leu Thr Leu Ser Lys Ala Asp Tyr Glu Lys His Lys Val Tyr 
            180                 185                 190         


Ala Cys Glu Val Thr His Gln Gly Leu Ser Ser Pro Val Thr Lys Ser 
        195                 200                 205             


Phe Asn Arg Gly Glu Cys 
    210                 


<210>  192
<211>  1344
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  DNA sequence encoding the human IgG1 HC of an altered anti-CTLA4 antibody 11F4 with the alterations G44R, Q105D, K147D, H168D, V173C, R255K, D399R, and K409E

<400>  192
caggtgcagc tggtggagtc cggcggtggt gtggtggagc ccggacgtag tctgcgtttg       60

tcctgcgctg cttccgggtt caccttctcc tcttatggaa tgcattgggt gcgtcaggcc      120

ccgggaaagc gcctggagtg ggtggccgtg atttggtaca agcccagcga gaaggactat      180

gctgacagcg ccaaagggag attcaccatc agccgcgaca acagcaagaa cacactctac      240

ctgcagatga acagtctccg cgctgaggac acagcagtct actactgtgc acgcggcggc      300

ctcctgggct atttcgacta ctggggcgac ggcaccctgg tgaccgtgag ctctgctagc      360

accaagggcc catcggtctt ccccctggca ccctcctcca agagcacctc tgggggcaca      420

gcggccctgg gctgcctggt cgacgactac ttccccgaac cggtgacggt gtcgtggaac      480

tcaggcgccc tgaccagcgg cgtggacacc ttcccggctt gcctacagtc ctcaggactc      540

tactccctca gcagcgtggt gaccgtgccc tccagcagct tgggcaccca gacctacatc      600

tgcaacgtga atcacaagcc cagcaacacc aaggtggaca agaaagttga gcccaaatct      660

tgtgacaaaa ctcacacatg cccaccgtgc ccagcacctg aactcctggg gggaccgtca      720

gtcttcctct tccccccaaa acccaaggac accctcatga tctccaagac ccctgaggtc      780

acatgcgtgg tggtggacgt gagccacgaa gaccctgagg tcaagttcaa ctggtacgtg      840

gacggcgtgg aggtgcataa tgccaagaca aagccgcggg aggagcagta caacagcacg      900

taccgtgtgg tcagcgtcct caccgtcctg caccaggact ggctgaatgg caaggagtac      960

aagtgcaagg tctccaacaa agccctccca gcccccatcg agaaaaccat ctccaaagcc     1020

aaagggcagc cccgagaacc acaggtgtac accctgcccc catcccggga ggagatgacc     1080

aagaaccagg tcagcctgac ctgcctggtc aaaggcttct atcccagcga catcgccgtg     1140

gagtgggaga gcaatgggca gccggagaac aactacaaga ccacgcctcc cgtgctgcgg     1200

tccgacggct ccttcttcct ctatagcgag ctcaccgtgg acaagagcag gtggcagcag     1260

gggaacgtct tctcatgctc cgtgatgcat gaggctctgc acaaccacta cacgcagaag     1320

agcctctccc tgtctccggg taaa                                            1344


<210>  193
<211>  447
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Amino acid sequence of the human IgG1 HC of an altered anti-CTLA4 antibody 11F4  with the alterations G44R, Q105D, K147D, H168D, V173C, R255K, D399R, and K409E

<400>  193

Gln Val Gln Leu Val Glu Ser Gly Gly Gly Val Val Glu Pro Gly Arg 
1               5                   10                  15      


Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Phe Thr Phe Ser Ser Tyr 
            20                  25                  30          


Gly Met His Trp Val Arg Gln Ala Pro Gly Lys Arg Leu Glu Trp Val 
        35                  40                  45              


Ala Val Ile Trp Tyr Lys Pro Ser Glu Lys Asp Tyr Ala Asp Ser Ala 
    50                  55                  60                  


Lys Gly Arg Phe Thr Ile Ser Arg Asp Asn Ser Lys Asn Thr Leu Tyr 
65                  70                  75                  80  


Leu Gln Met Asn Ser Leu Arg Ala Glu Asp Thr Ala Val Tyr Tyr Cys 
                85                  90                  95      


Ala Arg Gly Gly Leu Leu Gly Tyr Phe Asp Tyr Trp Gly Asp Gly Thr 
            100                 105                 110         


Leu Val Thr Val Ser Ser Ala Ser Thr Lys Gly Pro Ser Val Phe Pro 
        115                 120                 125             


Leu Ala Pro Ser Ser Lys Ser Thr Ser Gly Gly Thr Ala Ala Leu Gly 
    130                 135                 140                 


Cys Leu Val Asp Asp Tyr Phe Pro Glu Pro Val Thr Val Ser Trp Asn 
145                 150                 155                 160 


Ser Gly Ala Leu Thr Ser Gly Val Asp Thr Phe Pro Ala Cys Leu Gln 
                165                 170                 175     


Ser Ser Gly Leu Tyr Ser Leu Ser Ser Val Val Thr Val Pro Ser Ser 
            180                 185                 190         


Ser Leu Gly Thr Gln Thr Tyr Ile Cys Asn Val Asn His Lys Pro Ser 
        195                 200                 205             


Asn Thr Lys Val Asp Lys Lys Val Glu Pro Lys Ser Cys Asp Lys Thr 
    210                 215                 220                 


His Thr Cys Pro Pro Cys Pro Ala Pro Glu Leu Leu Gly Gly Pro Ser 
225                 230                 235                 240 


Val Phe Leu Phe Pro Pro Lys Pro Lys Asp Thr Leu Met Ile Ser Lys 
                245                 250                 255     


Thr Pro Glu Val Thr Cys Val Val Val Asp Val Ser His Glu Asp Pro 
            260                 265                 270         


Glu Val Lys Phe Asn Trp Tyr Val Asp Gly Val Glu Val His Asn Ala 
        275                 280                 285             


Lys Thr Lys Pro Arg Glu Glu Gln Tyr Asn Ser Thr Tyr Arg Val Val 
    290                 295                 300                 


Ser Val Leu Thr Val Leu His Gln Asp Trp Leu Asn Gly Lys Glu Tyr 
305                 310                 315                 320 


Lys Cys Lys Val Ser Asn Lys Ala Leu Pro Ala Pro Ile Glu Lys Thr 
                325                 330                 335     


Ile Ser Lys Ala Lys Gly Gln Pro Arg Glu Pro Gln Val Tyr Thr Leu 
            340                 345                 350         


Pro Pro Ser Arg Glu Glu Met Thr Lys Asn Gln Val Ser Leu Thr Cys 
        355                 360                 365             


Leu Val Lys Gly Phe Tyr Pro Ser Asp Ile Ala Val Glu Trp Glu Ser 
    370                 375                 380                 


Asn Gly Gln Pro Glu Asn Asn Tyr Lys Thr Thr Pro Pro Val Leu Arg 
385                 390                 395                 400 


Ser Asp Gly Ser Phe Phe Leu Tyr Ser Glu Leu Thr Val Asp Lys Ser 
                405                 410                 415     


Arg Trp Gln Gln Gly Asn Val Phe Ser Cys Ser Val Met His Glu Ala 
            420                 425                 430         


Leu His Asn His Tyr Thr Gln Lys Ser Leu Ser Leu Ser Pro Lys 
        435                 440                 445         


<210>  194
<211>  642
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  DNA sequence encoding the human LC of an altered anti-CTLA4 antibody 11F4  with the alterations V43R, G100D, S131R, Q160C, and S174R

<400>  194
gaaatcgtct tgacccaatc acctgggacc ctctccctgt cacctggaga gagagcaact       60

ttgagttgta gagctagtca gagtatcaac tcatacctgg cttggtatca gcagaagcct      120

gggcaaaggc cacgccctct catatatggt gtaagtagtc gtgctactgg catccccgac      180

cgattcagcg gctccggttc tggtacagat ttcaccctta caatcagtag gctggaacca      240

gaggactttg cagtctacta ttgccaacag tatggtcgtt accctttcac attcggggat      300

gggacaaagg tggatattaa acgtacggtg gctgcaccat ctgtcttcat cttcccgcca      360

tctgatgagc agttgaaatc tggaactgcc cgcgttgtgt gcctgctgaa taacttctat      420

cccagagagg ccaaagtaca gtggaaggtg gataacgccc tccaatcggg taactcctgc      480

gagagtgtca cagagcagga cagcaaggac agcacctaca ggctcagcag caccctgacg      540

ctgagcaaag cagactacga gaaacacaaa gtctacgcct gcgaagtcac ccatcagggc      600

ctgagctcgc ccgtcacaaa gagcttcaac aggggagagt gt                         642


<210>  195
<211>  214
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Amino acid sequence of the human LC of an altered anti-CTLA4 antibody 11F4 with the alterations V43R, G100D, S131R, Q160C, and S174R

<400>  195

Glu Ile Val Leu Thr Gln Ser Pro Gly Thr Leu Ser Leu Ser Pro Gly 
1               5                   10                  15      


Glu Arg Ala Thr Leu Ser Cys Arg Ala Ser Gln Ser Ile Asn Ser Tyr 
            20                  25                  30          


Leu Ala Trp Tyr Gln Gln Lys Pro Gly Gln Arg Pro Arg Pro Leu Ile 
        35                  40                  45              


Tyr Gly Val Ser Ser Arg Ala Thr Gly Ile Pro Asp Arg Phe Ser Gly 
    50                  55                  60                  


Ser Gly Ser Gly Thr Asp Phe Thr Leu Thr Ile Ser Arg Leu Glu Pro 
65                  70                  75                  80  


Glu Asp Phe Ala Val Tyr Tyr Cys Gln Gln Tyr Gly Arg Tyr Pro Phe 
                85                  90                  95      


Thr Phe Gly Asp Gly Thr Lys Val Asp Ile Lys Arg Thr Val Ala Ala 
            100                 105                 110         


Pro Ser Val Phe Ile Phe Pro Pro Ser Asp Glu Gln Leu Lys Ser Gly 
        115                 120                 125             


Thr Ala Arg Val Val Cys Leu Leu Asn Asn Phe Tyr Pro Arg Glu Ala 
    130                 135                 140                 


Lys Val Gln Trp Lys Val Asp Asn Ala Leu Gln Ser Gly Asn Ser Cys 
145                 150                 155                 160 


Glu Ser Val Thr Glu Gln Asp Ser Lys Asp Ser Thr Tyr Arg Leu Ser 
                165                 170                 175     


Ser Thr Leu Thr Leu Ser Lys Ala Asp Tyr Glu Lys His Lys Val Tyr 
            180                 185                 190         


Ala Cys Glu Val Thr His Gln Gly Leu Ser Ser Pro Val Thr Lys Ser 
        195                 200                 205             


Phe Asn Arg Gly Glu Cys 
    210                 


<210>  196
<211>  132
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Consensus amino acid sequence for a human VH 


<220>
<221>  X
<222>  (1)..(16)
<223>  X is any amino acid ordinarily found in living organisms

<220>
<221>  X
<222>  (17)..(17)
<223>  X is serine or threonine

<220>
<221>  X
<222>  (18)..(18)
<223>  X is valine or leucine

<220>
<221>  X
<222>  (19)..(19)
<223>  X is any amino acid ordinarily found in living organisms

<220>
<221>  X
<222>  (20)..(20)
<223>  X is valine or leucine

<220>
<221>  X
<222>  (21)..(21)
<223>  X is serine or threonine

<220>
<221>  X
<222>  (23)..(35)
<223>  X is any amino acid ordinarily found in living organisms

<220>
<221>  X
<222>  (36)..(37)
<223>  X may or may not be present, but, if present, can be any amino 
       acid ordinarily found in living organisms

<220>
<221>  X
<222>  (39)..(43)
<223>  X is any amino acid ordinarily found in living organisms

<220>
<221>  X
<222>  (45)..(45)
<223>  X is lysine or glutamine

<220>
<221>  X
<222>  (48)..(54)
<223>  X is any amino acid ordinarily found in living organisms

<220>
<221>  X
<222>  (55)..(57)
<223>  X may or may not be present, but, if present, can be any amino 
       acid ordinarily found in living organisms

<220>
<221>  X
<222>  (58)..(108)
<223>  X is any amino acid ordinarily found in living organisms

<220>
<221>  X
<222>  (109)..(119)
<223>  X may or may not be present, but, if present, can be any amino 
       acid ordinarily found in living organisms

<220>
<221>  X
<222>  (120)..(132)
<223>  X is any amino acid ordinarily found in living organisms

<400>  196

Xaa Xaa Xaa Leu Xaa Xaa Xaa Gly Xaa Xaa Xaa Xaa Xaa Pro Xaa Xaa 
1               5                   10                  15      


Xaa Xaa Xaa Xaa Xaa Cys Xaa Xaa Xaa Gly Xaa Xaa Xaa Xaa Xaa Xaa 
            20                  25                  30          


Xaa Xaa Xaa Xaa Xaa Trp Xaa Arg Gln Xaa Xaa Gly Xaa Gly Leu Xaa 
        35                  40                  45              


Trp Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa 
    50                  55                  60                  


Xaa Xaa Xaa Xaa Xaa Xaa Arg Xaa Xaa Xaa Xaa Xaa Xaa Xaa Ser Xaa 
65                  70                  75                  80  


Xaa Xaa Xaa Xaa Leu Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Asp Xaa Xaa 
                85                  90                  95      


Xaa Tyr Xaa Cys Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa 
            100                 105                 110         


Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Trp Xaa Gln Gly Xaa Xaa Val 
        115                 120                 125             


Xaa Val Ser Xaa 
    130         


<210>  197
<211>  98
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Consensus amino acid sequence for a CH1 


<220>
<221>  X
<222>  (1)..(15)
<223>  X is any amino acid ordinarily found in living organisms

<220>
<221>  X
<222>  (16)..(16)
<223>  X is arginine or lysine

<220>
<221>  X
<222>  (17)..(98)
<223>  X is any amino acid ordinarily found in living organisms

<400>  197

Xaa Xaa Xaa Xaa Xaa Pro Xaa Xaa Xaa Pro Xaa Xaa Xaa Xaa Xaa Xaa 
1               5                   10                  15      


Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Cys Leu Xaa Lys Xaa Xaa 
            20                  25                  30          


Xaa Pro Xaa Xaa Xaa Xaa Xaa Xaa Trp Xaa Xaa Xaa Xaa Xaa Xaa Xaa 
        35                  40                  45              


Xaa Xaa His Xaa Phe Xaa Xaa Val Xaa Xaa Xaa Xaa Xaa Xaa Xaa Thr 
    50                  55                  60                  


Xaa Ser Ser Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa 
65                  70                  75                  80  


Xaa Xaa Cys Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa 
                85                  90                  95      


Xaa Xaa 
        


<210>  198
<211>  98
<212>  PRT
<213>  Homo sapiens

<400>  198

Ala Ser Thr Lys Gly Pro Ser Val Phe Pro Leu Ala Pro Ser Ser Lys 
1               5                   10                  15      


Ser Thr Ser Gly Gly Thr Ala Ala Leu Gly Cys Leu Val Lys Asp Tyr 
            20                  25                  30          


Phe Pro Glu Pro Val Thr Val Ser Trp Asn Ser Gly Ala Leu Thr Ser 
        35                  40                  45              


Gly Val His Thr Phe Pro Ala Val Leu Gln Ser Ser Gly Leu Tyr Ser 
    50                  55                  60                  


Leu Ser Ser Val Val Thr Val Pro Ser Ser Ser Leu Gly Thr Gln Thr 
65                  70                  75                  80  


Tyr Ile Cys Asn Val Asn His Lys Pro Ser Asn Thr Lys Val Asp Lys 
                85                  90                  95      


Lys Val 
        


<210>  199
<211>  98
<212>  PRT
<213>  Homo sapiens

<400>  199

Ala Ser Thr Lys Gly Pro Ser Val Phe Pro Leu Ala Pro Cys Ser Arg 
1               5                   10                  15      


Ser Thr Ser Glu Ser Thr Ala Ala Leu Gly Cys Leu Val Lys Asp Tyr 
            20                  25                  30          


Phe Pro Glu Pro Val Thr Val Ser Trp Asn Ser Gly Ala Leu Thr Ser 
        35                  40                  45              


Gly Val His Thr Phe Pro Ala Val Leu Gln Ser Ser Gly Leu Tyr Ser 
    50                  55                  60                  


Leu Ser Ser Val Val Thr Val Pro Ser Ser Asn Phe Gly Thr Gln Thr 
65                  70                  75                  80  


Tyr Thr Cys Asn Val Asp His Lys Pro Ser Asn Thr Lys Val Asp Lys 
                85                  90                  95      


Thr Val 
        


<210>  200
<211>  98
<212>  PRT
<213>  Homo sapiens

<400>  200

Ala Ser Thr Lys Gly Pro Ser Val Phe Pro Leu Ala Pro Cys Ser Arg 
1               5                   10                  15      


Ser Thr Ser Gly Gly Thr Ala Ala Leu Gly Cys Leu Val Lys Asp Tyr 
            20                  25                  30          


Phe Pro Glu Pro Val Thr Val Ser Trp Asn Ser Gly Ala Leu Thr Ser 
        35                  40                  45              


Gly Val His Thr Phe Pro Ala Val Leu Gln Ser Ser Gly Leu Tyr Ser 
    50                  55                  60                  


Leu Ser Ser Val Val Thr Val Pro Ser Ser Ser Leu Gly Thr Gln Thr 
65                  70                  75                  80  


Tyr Thr Cys Asn Val Asn His Lys Pro Ser Asn Thr Lys Val Asp Lys 
                85                  90                  95      


Arg Val 
        


<210>  201
<211>  98
<212>  PRT
<213>  Homo sapiens

<400>  201

Ala Ser Thr Lys Gly Pro Ser Val Phe Pro Leu Ala Pro Cys Ser Arg 
1               5                   10                  15      


Ser Thr Ser Glu Ser Thr Ala Ala Leu Gly Cys Leu Val Lys Asp Tyr 
            20                  25                  30          


Phe Pro Glu Pro Val Thr Val Ser Trp Asn Ser Gly Ala Leu Thr Ser 
        35                  40                  45              


Gly Val His Thr Phe Pro Ala Val Leu Gln Ser Ser Gly Leu Tyr Ser 
    50                  55                  60                  


Leu Ser Ser Val Val Thr Val Pro Ser Ser Ser Leu Gly Thr Lys Thr 
65                  70                  75                  80  


Tyr Thr Cys Asn Val Asp His Lys Pro Ser Asn Thr Lys Val Asp Lys 
                85                  90                  95      


Arg Val 
        


<210>  202
<211>  232
<212>  PRT
<213>  Homo sapiens

<400>  202

Glu Pro Lys Ser Cys Asp Lys Thr His Thr Cys Pro Pro Cys Pro Ala 
1               5                   10                  15      


Pro Glu Leu Leu Gly Gly Pro Ser Val Phe Leu Phe Pro Pro Lys Pro 
            20                  25                  30          


Lys Asp Thr Leu Met Ile Ser Arg Thr Pro Glu Val Thr Cys Val Val 
        35                  40                  45              


Val Asp Val Ser His Glu Asp Pro Glu Val Lys Phe Asn Trp Tyr Val 
    50                  55                  60                  


Asp Gly Val Glu Val His Asn Ala Lys Thr Lys Pro Arg Glu Glu Gln 
65                  70                  75                  80  


Tyr Asn Ser Thr Tyr Arg Val Val Ser Val Leu Thr Val Leu His Gln 
                85                  90                  95      


Asp Trp Leu Asn Gly Lys Glu Tyr Lys Cys Lys Val Ser Asn Lys Ala 
            100                 105                 110         


Leu Pro Ala Pro Ile Glu Lys Thr Ile Ser Lys Ala Lys Gly Gln Pro 
        115                 120                 125             


Arg Glu Pro Gln Val Tyr Thr Leu Pro Pro Ser Arg Glu Glu Met Thr 
    130                 135                 140                 


Lys Asn Gln Val Ser Leu Thr Cys Leu Val Lys Gly Phe Tyr Pro Ser 
145                 150                 155                 160 


Asp Ile Ala Val Glu Trp Glu Ser Asn Gly Gln Pro Glu Asn Asn Tyr 
                165                 170                 175     


Lys Thr Thr Pro Pro Val Leu Asp Ser Asp Gly Ser Phe Phe Leu Tyr 
            180                 185                 190         


Ser Lys Leu Thr Val Asp Lys Ser Arg Trp Gln Gln Gly Asn Val Phe 
        195                 200                 205             


Ser Cys Ser Val Met His Glu Ala Leu His Asn His Tyr Thr Gln Lys 
    210                 215                 220                 


Ser Leu Ser Leu Ser Pro Gly Lys 
225                 230         


<210>  203
<211>  228
<212>  PRT
<213>  Homo sapiens

<400>  203

Glu Arg Lys Cys Cys Val Glu Cys Pro Pro Cys Pro Ala Pro Pro Val 
1               5                   10                  15      


Ala Gly Pro Ser Val Phe Leu Phe Pro Pro Lys Pro Lys Asp Thr Leu 
            20                  25                  30          


Met Ile Ser Arg Thr Pro Glu Val Thr Cys Val Val Val Asp Val Ser 
        35                  40                  45              


His Glu Asp Pro Glu Val Gln Phe Asn Trp Tyr Val Asp Gly Met Glu 
    50                  55                  60                  


Val His Asn Ala Lys Thr Lys Pro Arg Glu Glu Gln Phe Asn Ser Thr 
65                  70                  75                  80  


Phe Arg Val Val Ser Val Leu Thr Val Val His Gln Asp Trp Leu Asn 
                85                  90                  95      


Gly Lys Glu Tyr Lys Cys Lys Val Ser Asn Lys Gly Leu Pro Ala Pro 
            100                 105                 110         


Ile Glu Lys Thr Ile Ser Lys Thr Lys Gly Gln Pro Arg Glu Pro Gln 
        115                 120                 125             


Val Tyr Thr Leu Pro Pro Ser Arg Glu Glu Met Thr Lys Asn Gln Val 
    130                 135                 140                 


Ser Leu Thr Cys Leu Val Lys Gly Phe Tyr Pro Ser Asp Ile Ala Val 
145                 150                 155                 160 


Glu Trp Glu Ser Asn Gly Gln Pro Glu Asn Asn Tyr Lys Thr Thr Pro 
                165                 170                 175     


Pro Met Leu Asp Ser Asp Gly Ser Phe Phe Leu Tyr Ser Lys Leu Thr 
            180                 185                 190         


Val Asp Lys Ser Arg Trp Gln Gln Gly Asn Val Phe Ser Cys Ser Val 
        195                 200                 205             


Met His Glu Ala Leu His Asn His Tyr Thr Gln Lys Ser Leu Ser Leu 
    210                 215                 220                 


Ser Pro Gly Lys 
225             


<210>  204
<211>  279
<212>  PRT
<213>  Homo sapiens

<400>  204

Glu Leu Lys Thr Pro Leu Gly Asp Thr Thr His Thr Cys Pro Arg Cys 
1               5                   10                  15      


Pro Glu Pro Lys Ser Cys Asp Thr Pro Pro Pro Cys Pro Arg Cys Pro 
            20                  25                  30          


Glu Pro Lys Ser Cys Asp Thr Pro Pro Pro Cys Pro Arg Cys Pro Glu 
        35                  40                  45              


Pro Lys Ser Cys Asp Thr Pro Pro Pro Cys Pro Arg Cys Pro Ala Pro 
    50                  55                  60                  


Glu Leu Leu Gly Gly Pro Ser Val Phe Leu Phe Pro Pro Lys Pro Lys 
65                  70                  75                  80  


Asp Thr Leu Met Ile Ser Arg Thr Pro Glu Val Thr Cys Val Val Val 
                85                  90                  95      


Asp Val Ser His Glu Asp Pro Glu Val Gln Phe Lys Trp Tyr Val Asp 
            100                 105                 110         


Gly Val Glu Val His Asn Ala Lys Thr Lys Pro Arg Glu Glu Gln Tyr 
        115                 120                 125             


Asn Ser Thr Phe Arg Val Val Ser Val Leu Thr Val Leu His Gln Asp 
    130                 135                 140                 


Trp Leu Asn Gly Lys Glu Tyr Lys Cys Lys Val Ser Asn Lys Ala Leu 
145                 150                 155                 160 


Pro Ala Pro Ile Glu Lys Thr Ile Ser Lys Thr Lys Gly Gln Pro Arg 
                165                 170                 175     


Glu Pro Gln Val Tyr Thr Leu Pro Pro Ser Arg Glu Glu Met Thr Lys 
            180                 185                 190         


Asn Gln Val Ser Leu Thr Cys Leu Val Lys Gly Phe Tyr Pro Ser Asp 
        195                 200                 205             


Ile Ala Val Glu Trp Glu Ser Ser Gly Gln Pro Glu Asn Asn Tyr Asn 
    210                 215                 220                 


Thr Thr Pro Pro Met Leu Asp Ser Asp Gly Ser Phe Phe Leu Tyr Ser 
225                 230                 235                 240 


Lys Leu Thr Val Asp Lys Ser Arg Trp Gln Gln Gly Asn Ile Phe Ser 
                245                 250                 255     


Cys Ser Val Met His Glu Ala Leu His Asn Arg Phe Thr Gln Lys Ser 
            260                 265                 270         


Leu Ser Leu Ser Pro Gly Lys 
        275                 


<210>  205
<211>  229
<212>  PRT
<213>  Homo sapiens

<400>  205

Glu Ser Lys Tyr Gly Pro Pro Cys Pro Ser Cys Pro Ala Pro Glu Phe 
1               5                   10                  15      


Leu Gly Gly Pro Ser Val Phe Leu Phe Pro Pro Lys Pro Lys Asp Thr 
            20                  25                  30          


Leu Met Ile Ser Arg Thr Pro Glu Val Thr Cys Val Val Val Asp Val 
        35                  40                  45              


Ser Gln Glu Asp Pro Glu Val Gln Phe Asn Trp Tyr Val Asp Gly Val 
    50                  55                  60                  


Glu Val His Asn Ala Lys Thr Lys Pro Arg Glu Glu Gln Phe Asn Ser 
65                  70                  75                  80  


Thr Tyr Arg Val Val Ser Val Leu Thr Val Leu His Gln Asp Trp Leu 
                85                  90                  95      


Asn Gly Lys Glu Tyr Lys Cys Lys Val Ser Asn Lys Gly Leu Pro Ser 
            100                 105                 110         


Ser Ile Glu Lys Thr Ile Ser Lys Ala Lys Gly Gln Pro Arg Glu Pro 
        115                 120                 125             


Gln Val Tyr Thr Leu Pro Pro Ser Gln Glu Glu Met Thr Lys Asn Gln 
    130                 135                 140                 


Val Ser Leu Thr Cys Leu Val Lys Gly Phe Tyr Pro Ser Asp Ile Ala 
145                 150                 155                 160 


Val Glu Trp Glu Ser Asn Gly Gln Pro Glu Asn Asn Tyr Lys Thr Thr 
                165                 170                 175     


Pro Pro Val Leu Asp Ser Asp Gly Ser Phe Phe Leu Tyr Ser Arg Leu 
            180                 185                 190         


Thr Val Asp Lys Ser Arg Trp Gln Glu Gly Asn Val Phe Ser Cys Ser 
        195                 200                 205             


Val Met His Glu Ala Leu His Asn His Tyr Thr Gln Lys Ser Leu Ser 
    210                 215                 220                 


Leu Ser Leu Gly Lys 
225                 


<210>  206
<211>  117
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Consensus amino acid sequence for a human VL 


<220>
<221>  X
<222>  (1)..(27)
<223>  X is any amino acid ordinarily found in living organisms

<220>
<221>  X
<222>  (28)..(33)
<223>  X may or may not be present, but, if present, can be any amino 
       acid ordinarily found in living organisms

<220>
<221>  X
<222>  (34)..(48)
<223>  X is any amino acid ordinarily found in living organisms

<220>
<221>  X
<222>  (49)..(49)
<223>  X is alanine, serine, or proline

<220>
<221>  X
<222>  (51)..(63)
<223>  X is any amino acid ordinarily found in living organisms

<220>
<221>  X
<222>  (64)..(64)
<223>  X is isoleucine or valine

<220>
<221>  X
<222>  (66)..(89)
<223>  X is any amino acid ordinarily found in living organisms

<220>
<221>  X
<222>  (90)..(90)
<223>  X is alanine or glycine

<220>
<221>  X
<222>  (91)..(91)
<223>  X is any amino acid ordinarily found in living organisms

<220>
<221>  X
<222>  (93)..(93)
<223>  X is tyrosine or phenylalanine

<220>
<221>  X
<222>  (94)..(101)
<223>  X is any amino acid ordinarily found in living organisms

<220>
<221>  X
<222>  (102)..(102)
<223>  X may or may not be present, but, if present, can be any amino 
       acid ordinarily found in living organisms

<220>
<221>  X
<222>  (103)..(104)
<223>  X is any amino acid ordinarily found in living organisms

<220>
<221>  X
<222>  (107)..(107)
<223>  X is glutamine or glycine

<220>
<221>  X
<222>  (110)..(117)
<223>  X is any amino acid ordinarily found in living organisms

<400>  206

Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Gly 
1               5                   10                  15      


Xaa Xaa Xaa Xaa Xaa Xaa Cys Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa 
            20                  25                  30          


Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Trp Xaa Xaa Xaa Xaa Xaa Xaa Xaa 
        35                  40                  45              


Xaa Pro Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa 
    50                  55                  60                  


Pro Xaa Arg Phe Ser Gly Ser Xaa Xaa Xaa Xaa Xaa Xaa Xaa Leu Xaa 
65                  70                  75                  80  


Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Tyr Xaa Xaa Xaa Xaa 
                85                  90                  95      


Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Phe Gly Xaa Gly Thr Xaa Xaa Xaa 
            100                 105                 110         


Xaa Xaa Xaa Xaa Xaa 
        115         


<210>  207
<211>  107
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Consensus amino acid sequence for a CL kappa 


<220>
<221>  X
<222>  (1)..(107)
<223>  X is any amino acid ordinarily found in living organisms

<400>  207

Xaa Xaa Xaa Xaa Xaa Pro Xaa Xaa Xaa Xaa Xaa Pro Pro Xaa Xaa Xaa 
1               5                   10                  15      


Xaa Xaa Xaa Xaa Xaa Xaa Xaa Ser Xaa Val Cys Xaa Xaa Xaa Xaa Xaa 
            20                  25                  30          


Xaa Pro Xaa Xaa Xaa Xaa Val Xaa Trp Xaa Xaa Xaa Xaa Xaa Xaa Xaa 
        35                  40                  45              


Xaa Xaa Xaa Xaa Gln Xaa Ser Xaa Thr Xaa Xaa Xaa Xaa Xaa Xaa Xaa 
    50                  55                  60                  


Xaa Xaa Ser Xaa Ser Ser Thr Leu Thr Leu Xaa Xaa Xaa Xaa Xaa Xaa 
65                  70                  75                  80  


Xaa Xaa Xaa Xaa Xaa Xaa Cys Xaa Xaa Xaa His Xaa Xaa Xaa Xaa Xaa 
                85                  90                  95      


Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Cys 
            100                 105         


<210>  208
<211>  107
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Consensus amino acid sequence for a CL lambda 


<220>
<221>  X
<222>  (1)..(66)
<223>  X is any amino acid ordinarily found in living organisms

<220>
<221>  X
<222>  (67)..(67)
<223>  X is alanine or methionine

<220>
<221>  X
<222>  (68)..(106)
<223>  X is any amino acid ordinarily found in living organisms

<400>  208

Xaa Xaa Xaa Xaa Xaa Pro Xaa Xaa Xaa Xaa Xaa Pro Pro Xaa Xaa Xaa 
1               5                   10                  15      


Xaa Xaa Xaa Xaa Xaa Xaa Xaa Ala Xaa Val Cys Xaa Xaa Xaa Xaa Xaa 
            20                  25                  30          


Xaa Pro Xaa Xaa Xaa Xaa Val Xaa Trp Xaa Xaa Xaa Xaa Xaa Xaa Xaa 
        35                  40                  45              


Xaa Xaa Xaa Xaa Glu Xaa Thr Xaa Pro Xaa Xaa Xaa Xaa Xaa Xaa Xaa 
    50                  55                  60                  


Xaa Xaa Xaa Xaa Ser Ser Tyr Leu Ser Leu Xaa Xaa Xaa Xaa Xaa Xaa 
65                  70                  75                  80  


Xaa Xaa Xaa Xaa Xaa Xaa Cys Xaa Xaa Xaa His Xaa Xaa Xaa Xaa Xaa 
                85                  90                  95      


Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Cys 
            100                 105         




