                               SEQUENCE LISTING

<110> MEDIMMUNE LLC
 
<120> SIGNAL POLYPEPTIDE FOR IMPROVED SECRETION OF PROTEIN

<130> NSP4-100-WO-PCT

<140> US 62/413,607
<141> 2016-10-27

<160> 30    

<170> PatentIn version 3.5

<210> 1
<211> 20
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      peptide

<400> 1
Met Lys Lys Ile Thr Ala Ala Ala Gly Leu Leu Leu Leu Ala Ala Gln 
1               5                   10                  15      


Pro Ala Met Ala 
            20  


<210> 2
<211> 313
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      polypeptide

<400> 2
Met Lys Lys Ile Thr Ala Ala Ala Gly Leu Leu Leu Leu Ala Ala Gln 
1               5                   10                  15      


Pro Ala Met Ala Ala Asp Ser Asp Ile Asn Ile Lys Thr Gly Thr Thr 
            20                  25                  30          


Asp Ile Gly Ser Asn Thr Thr Val Lys Thr Gly Asp Leu Val Thr Tyr 
        35                  40                  45              


Asp Lys Glu Asn Gly Met Leu Lys Lys Val Phe Tyr Ser Phe Ile Asp 
    50                  55                  60                  


Asp Lys Asn His Asn Lys Lys Leu Leu Val Ile Arg Thr Lys Gly Thr 
65                  70                  75                  80  


Ile Ala Gly Gln Tyr Arg Val Tyr Ser Glu Glu Gly Ala Asn Lys Ser 
                85                  90                  95      


Gly Leu Ala Trp Pro Ser Ala Phe Lys Val Gln Leu Gln Leu Pro Asp 
            100                 105                 110         


Asn Glu Val Ala Gln Ile Ser Asp Tyr Tyr Pro Arg Asn Ser Ile Asp 
        115                 120                 125             


Thr Lys Glu Tyr Met Ser Thr Leu Thr Tyr Gly Phe Asn Gly Asn Val 
    130                 135                 140                 


Thr Gly Asp Asp Thr Gly Lys Ile Gly Gly Leu Ile Gly Ala Asn Val 
145                 150                 155                 160 


Ser Ile Gly His Thr Leu Lys Tyr Val Gln Pro Asp Phe Lys Thr Ile 
                165                 170                 175     


Leu Glu Ser Pro Thr Asp Lys Lys Val Gly Trp Lys Val Ile Phe Asn 
            180                 185                 190         


Asn Met Val Asn Gln Asn Trp Gly Pro Tyr Asp Arg Asp Ser Trp Asn 
        195                 200                 205             


Pro Val Tyr Gly Asn Gln Leu Phe Met Lys Thr Arg Asn Gly Ser Met 
    210                 215                 220                 


Lys Ala Ala Asp Asn Phe Leu Asp Pro Asn Lys Ala Ser Ser Leu Leu 
225                 230                 235                 240 


Ser Ser Gly Phe Ser Pro Asp Phe Ala Thr Val Ile Thr Met Asp Arg 
                245                 250                 255     


Lys Ala Ser Lys Gln Gln Thr Asn Ile Asp Val Ile Tyr Glu Arg Val 
            260                 265                 270         


Arg Asp Asp Tyr Gln Leu His Trp Thr Ser Thr Asn Trp Lys Gly Thr 
        275                 280                 285             


Asn Thr Lys Asp Lys Trp Ile Asp Arg Ser Ser Glu Arg Tyr Lys Ile 
    290                 295                 300                 


Asp Trp Glu Lys Glu Glu Met Thr Asn 
305                 310             


<210> 3
<211> 293
<212> PRT
<213> Staphylococcus aureus

<400> 3
Ala Asp Ser Asp Ile Asn Ile Lys Thr Gly Thr Thr Asp Ile Gly Ser 
1               5                   10                  15      


Asn Thr Thr Val Lys Thr Gly Asp Leu Val Thr Tyr Asp Lys Glu Asn 
            20                  25                  30          


Gly Met His Lys Lys Val Phe Tyr Ser Phe Ile Asp Asp Lys Asn His 
        35                  40                  45              


Asn Lys Lys Leu Leu Val Ile Arg Thr Lys Gly Thr Ile Ala Gly Gln 
    50                  55                  60                  


Tyr Arg Val Tyr Ser Glu Glu Gly Ala Asn Lys Ser Gly Leu Ala Trp 
65                  70                  75                  80  


Pro Ser Ala Phe Lys Val Gln Leu Gln Leu Pro Asp Asn Glu Val Ala 
                85                  90                  95      


Gln Ile Ser Asp Tyr Tyr Pro Arg Asn Ser Ile Asp Thr Lys Glu Tyr 
            100                 105                 110         


Met Ser Thr Leu Thr Tyr Gly Phe Asn Gly Asn Val Thr Gly Asp Asp 
        115                 120                 125             


Thr Gly Lys Ile Gly Gly Leu Ile Gly Ala Asn Val Ser Ile Gly His 
    130                 135                 140                 


Thr Leu Lys Tyr Val Gln Pro Asp Phe Lys Thr Ile Leu Glu Ser Pro 
145                 150                 155                 160 


Thr Asp Lys Lys Val Gly Trp Lys Val Ile Phe Asn Asn Met Val Asn 
                165                 170                 175     


Gln Asn Trp Gly Pro Tyr Asp Arg Asp Ser Trp Asn Pro Val Tyr Gly 
            180                 185                 190         


Asn Gln Leu Phe Met Lys Thr Arg Asn Gly Ser Met Lys Ala Ala Asp 
        195                 200                 205             


Asn Phe Leu Asp Pro Asn Lys Ala Ser Ser Leu Leu Ser Ser Gly Phe 
    210                 215                 220                 


Ser Pro Asp Phe Ala Thr Val Ile Thr Met Asp Arg Lys Ala Ser Lys 
225                 230                 235                 240 


Gln Gln Thr Asn Ile Asp Val Ile Tyr Glu Arg Val Arg Asp Asp Tyr 
                245                 250                 255     


Gln Leu His Trp Thr Ser Thr Asn Trp Lys Gly Thr Asn Thr Lys Asp 
            260                 265                 270         


Lys Trp Ile Asp Arg Ser Ser Glu Arg Tyr Lys Ile Asp Trp Glu Lys 
        275                 280                 285             


Glu Glu Met Thr Asn 
    290             


<210> 4
<211> 293
<212> PRT
<213> Staphylococcus aureus

<400> 4
Ala Asp Ser Asp Ile Asn Ile Lys Thr Gly Thr Thr Asp Ile Gly Ser 
1               5                   10                  15      


Asn Thr Thr Val Lys Thr Gly Asp Leu Val Thr Tyr Asp Lys Glu Asn 
            20                  25                  30          


Gly Met Leu Lys Lys Val Phe Tyr Ser Phe Ile Asp Asp Lys Asn His 
        35                  40                  45              


Asn Lys Lys Leu Leu Val Ile Arg Thr Lys Gly Thr Ile Ala Gly Gln 
    50                  55                  60                  


Tyr Arg Val Tyr Ser Glu Glu Gly Ala Asn Lys Ser Gly Leu Ala Trp 
65                  70                  75                  80  


Pro Ser Ala Phe Lys Val Gln Leu Gln Leu Pro Asp Asn Glu Val Ala 
                85                  90                  95      


Gln Ile Ser Asp Tyr Tyr Pro Arg Asn Ser Ile Asp Thr Lys Glu Tyr 
            100                 105                 110         


Met Ser Thr Leu Thr Tyr Gly Phe Asn Gly Asn Val Thr Gly Asp Asp 
        115                 120                 125             


Thr Gly Lys Ile Gly Gly Leu Ile Gly Ala Asn Val Ser Ile Gly His 
    130                 135                 140                 


Thr Leu Lys Tyr Val Gln Pro Asp Phe Lys Thr Ile Leu Glu Ser Pro 
145                 150                 155                 160 


Thr Asp Lys Lys Val Gly Trp Lys Val Ile Phe Asn Asn Met Val Asn 
                165                 170                 175     


Gln Asn Trp Gly Pro Tyr Asp Arg Asp Ser Trp Asn Pro Val Tyr Gly 
            180                 185                 190         


Asn Gln Leu Phe Met Lys Thr Arg Asn Gly Ser Met Lys Ala Ala Asp 
        195                 200                 205             


Asn Phe Leu Asp Pro Asn Lys Ala Ser Ser Leu Leu Ser Ser Gly Phe 
    210                 215                 220                 


Ser Pro Asp Phe Ala Thr Val Ile Thr Met Asp Arg Lys Ala Ser Lys 
225                 230                 235                 240 


Gln Gln Thr Asn Ile Asp Val Ile Tyr Glu Arg Val Arg Asp Asp Tyr 
                245                 250                 255     


Gln Leu His Trp Thr Ser Thr Asn Trp Lys Gly Thr Asn Thr Lys Asp 
            260                 265                 270         


Lys Trp Ile Asp Arg Ser Ser Glu Arg Tyr Lys Ile Asp Trp Glu Lys 
        275                 280                 285             


Glu Glu Met Thr Asn 
    290             


<210> 5
<211> 882
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      polynucleotide

<400> 5
gcagacagcg acatcaacat taagactggt accaccgaca tcggcagcaa tacgaccgtt       60

aaaaccggcg acctggtgac ctacgataaa gagaatggca tgttgaaaaa agttttctac      120

tcttttatcg atgataagaa tcacaacaaa aagctgctgg tcattcgtac gaagggcacc      180

atcgcgggtc agtatcgcgt ctactccgaa gagggcgcga acaagagcgg tctggcttgg      240

ccgagcgcat ttaaggtcca gctgcaactg cctgataacg aagttgcgca gattagcgac      300

tactacccac gcaatagcat tgacaccaaa gagtatatga gcaccctgac gtatggcttc      360

aatggtaacg tgaccggcga cgacacgggt aagatcggtg gtctgatcgg cgccaatgtg      420

agcatcggtc atacgctgaa atatgttcag ccggacttca agacgatttt ggagtccccg      480

acggacaaaa aagttggctg gaaagtgatt ttcaacaaca tggtcaatca aaattggggt      540

ccgtacgatc gtgacagctg gaacccggtg tatggtaatc aactgtttat gaaaacccgc      600

aacggttcta tgaaagcggc cgacaacttc ctggatccga ataaggctag ctccctgctg      660

tcgagcggtt ttagcccgga ttttgcaacg gtgattacca tggaccgtaa ggcgagcaag      720

caacagacca atatcgacgt catttacgaa cgtgttcgtg atgattatca gctgcactgg      780

actagcacca actggaaggg taccaacacc aaggataaat ggattgatcg ctcaagcgaa      840

cgttacaaga tcgattggga gaaagaagag atgacgaact aa                         882


<210> 6
<211> 19
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      peptide

<400> 6
Met Lys Lys Ile Trp Leu Ala Leu Ala Gly Leu Val Leu Ala Phe Ser 
1               5                   10                  15      


Ala Ser Ala 
            


<210> 7
<211> 22
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      peptide

<400> 7
Met Lys Tyr Leu Leu Pro Thr Ala Ala Ala Gly Leu Leu Leu Leu Ala 
1               5                   10                  15      


Ala Gln Pro Ala Met Ala 
            20          


<210> 8
<211> 21
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      peptide

<400> 8
Met Lys Gln Ser Thr Ile Ala Leu Ala Leu Leu Pro Leu Leu Phe Thr 
1               5                   10                  15      


Pro Val Thr Lys Ala 
            20      


<210> 9
<211> 26
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      peptide

<400> 9
Met Lys Thr His Ile Val Ser Ser Val Thr Thr Thr Leu Leu Leu Gly 
1               5                   10                  15      


Ser Ile Leu Met Asn Pro Val Ala Asn Ala 
            20                  25      


<210> 10
<211> 21
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      peptide

<400> 10
Met Lys Tyr Leu Leu Pro Trp Leu Ala Leu Ala Gly Leu Val Leu Ala 
1               5                   10                  15      


Phe Ser Ala Ser Ala 
            20      


<210> 11
<211> 20
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      peptide

<400> 11
Met Lys Lys Ile Thr Ala Ala Ala Gly Leu Leu Leu Leu Ala Ala Phe 
1               5                   10                  15      


Ser Ala Ser Ala 
            20  


<210> 12
<211> 19
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      peptide

<400> 12
Met Lys Lys Ile Trp Leu Ala Leu Ala Gly Leu Val Leu Ala Gln Pro 
1               5                   10                  15      


Ala Met Ala 
            


<210> 13
<211> 19
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      peptide

<400> 13
Met Lys Lys Ile Leu Val Leu Gly Ala Leu Ala Leu Trp Ala Gln Pro 
1               5                   10                  15      


Ala Met Ala 
            


<210> 14
<211> 19
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      peptide

<400> 14
Met Lys Lys Ile Trp Leu Ala Leu Val Leu Leu Ala Gly Ala Gln Pro 
1               5                   10                  15      


Ala Met Ala 
            


<210> 15
<211> 19
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      peptide

<400> 15
Met Lys Lys Ile Leu Ala Gly Trp Leu Ala Leu Val Leu Ala Gln Pro 
1               5                   10                  15      


Ala Met Ala 
            


<210> 16
<211> 19
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      peptide

<400> 16
Met Lys Lys Ile Leu Val Leu Leu Ala Gly Trp Leu Ala Ala Gln Pro 
1               5                   10                  15      


Ala Met Ala 
            


<210> 17
<211> 20
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      peptide

<400> 17
Met Lys Lys Ile Leu Leu Leu Leu Gly Thr Ala Ala Ala Ala Ala Gln 
1               5                   10                  15      


Pro Ala Met Ala 
            20  


<210> 18
<211> 20
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      peptide

<400> 18
Met Lys Lys Ile Leu Leu Leu Leu Leu Leu Leu Leu Leu Leu Ala Gln 
1               5                   10                  15      


Pro Ala Met Ala 
            20  


<210> 19
<211> 20
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      peptide

<400> 19
Met Lys Lys Ile Ala Ala Ala Ala Ala Ala Ala Ala Ala Ala Ala Gln 
1               5                   10                  15      


Pro Ala Met Ala 
            20  


<210> 20
<211> 21
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      peptide

<400> 20
Met Lys Tyr Leu Leu Pro Trp Leu Ala Leu Ala Gly Leu Val Leu Ala 
1               5                   10                  15      


Gln Pro Ala Met Ala 
            20      


<210> 21
<211> 22
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      peptide

<400> 21
Met Lys Tyr Leu Leu Pro Thr Ala Ala Ala Gly Leu Leu Leu Leu Ala 
1               5                   10                  15      


Ala Phe Ser Ala Ser Ala 
            20          


<210> 22
<211> 6
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      6xHis tag

<400> 22
His His His His His His 
1               5       


<210> 23
<211> 10
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      peptide

<400> 23
Leu Leu Leu Leu Gly Thr Ala Ala Ala Ala 
1               5                   10  


<210> 24
<211> 10
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      peptide

<400> 24
Leu Leu Leu Leu Leu Leu Leu Leu Leu Leu 
1               5                   10  


<210> 25
<211> 10
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      peptide

<400> 25
Ala Ala Ala Ala Ala Ala Ala Ala Ala Ala 
1               5                   10  


<210> 26
<211> 9
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      peptide

<400> 26
Leu Val Leu Gly Ala Leu Ala Leu Trp 
1               5                   


<210> 27
<211> 9
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      peptide

<400> 27
Trp Leu Ala Leu Val Leu Leu Ala Gly 
1               5                   


<210> 28
<211> 9
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      peptide

<400> 28
Leu Ala Gly Trp Leu Ala Leu Val Leu 
1               5                   


<210> 29
<211> 9
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      peptide

<400> 29
Leu Val Leu Leu Ala Gly Trp Leu Ala 
1               5                   


<210> 30
<211> 9
<212> PRT
<213> Staphylococcus aureus

<400> 30
Ala Asp Ser Asp Ile Asn Ile Lys Thr 
1               5                   

