                         SEQUENCE LISTING

<110>  University of Florida Research Foundation, Inc.
 
<120>  METHODS OF IDENTIFYING BIOLOGICALLY ACTIVE RANDOM PEPTIDES IN 
       PROCARYOTIC CELLS AND LIBRARIES OF PROCARYOTIC CELLS EXPRESSING 
       CANDIDATE BIOLOGICALLY ACTIVE RANDOM PEPTIDES

<130>  222109-2320

<160>  22    

<170>  PatentIn version 3.5

<210>  1
<211>  33
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  chemically synthesized nucleotide sequence of a test nucleic acid
       encoding a random peptide sequence for a candidate BARP


<220>
<221>  misc_feature
<222>  (10)..(27)
<223>  n is any nucleotide

<400>  1
atggcctgtn nnnnnnnnnn nnnnnnntgt tag                                    33


<210>  2
<211>  33
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  chemically synthesized nucleotide sequence of control BARP 
       6AA-6associated with normal growth and competency

<400>  2
atggcctgtg tgcgggtgtg gatggggtgt tag                                    33


<210>  3
<211>  33
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  chemically synthesized nucleotide sequence of control BARP 
       RBS6AA-11 associated with normal growth and competency

<400>  3
atggcctgtt acaccggtca tgctacttgt tag                                    33


<210>  4
<211>  51
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  chemically synthesized nucleotide sequence of BARP 12AA-1, -3, 
       and -4 associated with less blue phenotype

<400>  4
atgggctgtt ttcaacgaac cggttgtctt ggcacggtgc gatagtgtta g                51


<210>  5
<211>  51
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  chemically synthesized nucleotide sequence of BARP 12AA-2, -5, 
       -7, and -10 associated with mucoid growth and defects in 
       autolysis

<400>  5
atgggctgtg tggcagggcc ctgcggcctg acggcgcgta acttttgtta g                51


<210>  6
<211>  51
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  chemically synthesized nucleotide sequence of BARP 12AA-6 
       associated with mucoid growth and normal competency

<400>  6
atgggctgtt tccaaagtgt tcagcagctt cctctcgttc tcaaatgtta g                51


<210>  7
<211>  51
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  chemically synthesized nucleotide sequence of BARP 12AA-11 
       associated with mucoid growth and normal competency

<400>  7
atgggctgtt atgggtcttt tacactacgt ttcatttgaa atgattgtta g                51


<210>  8
<211>  33
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  chemically synthesized nucleotide sequence of BARP RBS6AA-1 
       associated with mucoid growth and normal competency

<400>  8
atggcctgta ttttatgcca tcaattatgt tag                                    33


<210>  9
<211>  33
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  chemically synthesized nucleotide sequence of BARP RBS6AA-10 
       associated with mucoid growth and normal competency

<400>  9
atggcctgtg cccctgttgt taataagtgt tag                                    33


<210>  10
<211>  33
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  chemically synthesized nucleotide sequence of BARP RBS6AA-12 
       associated with mucoid growth and normal competency

<400>  10
atggcctgtt gacaatttgt ccttaaatgt tag                                    33


<210>  11
<211>  51
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  chemically synthesized nucleotide sequence of BARP RBS12AA-5 
       associated with mucoid growth and less blue phenotype

<400>  11
atgggctgtt ttattgggtt cttgttccca cagtatgaac ttgtttgtta g                51


<210>  12
<211>  51
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  chemically synthesized nucleotide sequence of BARP RBS12AA-9 
       associated with mucoid growth and less blue phenotype

<400>  12
atgggctgtc acacttttat ttaaccggct cagtcttgct gactatgtta g                51


<210>  13
<211>  10
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  chemically synthesized peptide sequence of 6AA-6, SEQ ID NO: 2

<400>  13

Met Ala Cys Val Arg Val Trp Met Gly Cys 
1               5                   10  


<210>  14
<211>  10
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  chemically synthesized peptide sequence of RBS6AA-11, SEQ ID NO: 
       3

<400>  14

Met Ala Cys Tyr Thr Gly His Ala Thr Cys 
1               5                   10  


<210>  15
<211>  14
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  chemically synthesized peptide sequence of 12AA-1, -3, and -4, 
       SEQ ID NO: 4

<400>  15

Met Gly Cys Phe Gln Arg Thr Gly Cys Leu Gly Thr Val Arg 
1               5                   10                  


<210>  16
<211>  16
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  chemically synthesized peptide sequence of 12AA-2, -5, -7, and 
       -10, SEQ ID NO: 5

<400>  16

Met Gly Cys Val Ala Gly Pro Cys Gly Leu Thr Ala Arg Asn Phe Cys 
1               5                   10                  15      


<210>  17
<211>  16
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  chemically synthesized peptide sequence of 12AA-6, SEQ ID NO: 6

<400>  17

Met Gly Cys Phe Gln Ser Val Gln Gln Leu Pro Leu Val Leu Lys Cys 
1               5                   10                  15      


<210>  18
<211>  12
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  chemically synthesized peptide sequence of 12AA-11, SEQ ID NO: 7

<400>  18

Met Gly Cys Tyr Gly Ser Phe Thr Leu Arg Phe Ile 
1               5                   10          


<210>  19
<211>  10
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  chemically synthesized peptide sequence of RBS6AA-1, SEQ ID NO: 8

<400>  19

Met Ala Cys Ile Leu Cys His Gln Leu Cys 
1               5                   10  


<210>  20
<211>  10
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  chemically synthesized peptide sequence of RBS6AA-10, SEQ ID NO: 
       9

<400>  20

Met Ala Cys Ala Pro Val Val Asn Lys Cys 
1               5                   10  


<210>  21
<211>  16
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  chemically synthesized peptide sequence of RBS12AA-5, SEQ ID NO: 
       11

<400>  21

Met Gly Cys Phe Ile Gly Phe Leu Phe Pro Gln Tyr Glu Leu Val Cys 
1               5                   10                  15      


<210>  22
<211>  7
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  chemically synthesized peptide sequence of RBS12AA-9, SEQ ID NO: 
       12

<400>  22

Met Gly Cys His Thr Phe Ile 
1               5           


