                         SEQUENCE LISTING

<110>  Novozymes A/S
       Shen, Xinyu
       Borch, Kim
       Schroeder, Justin
 
<120>  METHODS OF DEFOAMING ETHANOL FERMENTATION

<130>  14288-WO-PCT

<160>  8     

<170>  PatentIn version 3.5

<210>  1
<211>  515
<212>  PRT
<213>  Bacillus stearothermophilus


<220>
<221>  mat_peptide
<222>  (1)..(515)

<400>  1

Ala Ala Pro Phe Asn Gly Thr Met Met Gln Tyr Phe Glu Trp Tyr Leu 
1               5                   10                  15      


Pro Asp Asp Gly Thr Leu Trp Thr Lys Val Ala Asn Glu Ala Asn Asn 
            20                  25                  30          


Leu Ser Ser Leu Gly Ile Thr Ala Leu Trp Leu Pro Pro Ala Tyr Lys 
        35                  40                  45              


Gly Thr Ser Arg Ser Asp Val Gly Tyr Gly Val Tyr Asp Leu Tyr Asp 
    50                  55                  60                  


Leu Gly Glu Phe Asn Gln Lys Gly Thr Val Arg Thr Lys Tyr Gly Thr 
65                  70                  75                  80  


Lys Ala Gln Tyr Leu Gln Ala Ile Gln Ala Ala His Ala Ala Gly Met 
                85                  90                  95      


Gln Val Tyr Ala Asp Val Val Phe Asp His Lys Gly Gly Ala Asp Gly 
            100                 105                 110         


Thr Glu Trp Val Asp Ala Val Glu Val Asn Pro Ser Asp Arg Asn Gln 
        115                 120                 125             


Glu Ile Ser Gly Thr Tyr Gln Ile Gln Ala Trp Thr Lys Phe Asp Phe 
    130                 135                 140                 


Pro Gly Arg Gly Asn Thr Tyr Ser Ser Phe Lys Trp Arg Trp Tyr His 
145                 150                 155                 160 


Phe Asp Gly Val Asp Trp Asp Glu Ser Arg Lys Leu Ser Arg Ile Tyr 
                165                 170                 175     


Lys Phe Arg Gly Ile Gly Lys Ala Trp Asp Trp Glu Val Asp Thr Glu 
            180                 185                 190         


Asn Gly Asn Tyr Asp Tyr Leu Met Tyr Ala Asp Leu Asp Met Asp His 
        195                 200                 205             


Pro Glu Val Val Thr Glu Leu Lys Asn Trp Gly Lys Trp Tyr Val Asn 
    210                 215                 220                 


Thr Thr Asn Ile Asp Gly Phe Arg Leu Asp Ala Val Lys His Ile Lys 
225                 230                 235                 240 


Phe Ser Phe Phe Pro Asp Trp Leu Ser Tyr Val Arg Ser Gln Thr Gly 
                245                 250                 255     


Lys Pro Leu Phe Thr Val Gly Glu Tyr Trp Ser Tyr Asp Ile Asn Lys 
            260                 265                 270         


Leu His Asn Tyr Ile Thr Lys Thr Asn Gly Thr Met Ser Leu Phe Asp 
        275                 280                 285             


Ala Pro Leu His Asn Lys Phe Tyr Thr Ala Ser Lys Ser Gly Gly Ala 
    290                 295                 300                 


Phe Asp Met Arg Thr Leu Met Thr Asn Thr Leu Met Lys Asp Gln Pro 
305                 310                 315                 320 


Thr Leu Ala Val Thr Phe Val Asp Asn His Asp Thr Glu Pro Gly Gln 
                325                 330                 335     


Ala Leu Gln Ser Trp Val Asp Pro Trp Phe Lys Pro Leu Ala Tyr Ala 
            340                 345                 350         


Phe Ile Leu Thr Arg Gln Glu Gly Tyr Pro Cys Val Phe Tyr Gly Asp 
        355                 360                 365             


Tyr Tyr Gly Ile Pro Gln Tyr Asn Ile Pro Ser Leu Lys Ser Lys Ile 
    370                 375                 380                 


Asp Pro Leu Leu Ile Ala Arg Arg Asp Tyr Ala Tyr Gly Thr Gln His 
385                 390                 395                 400 


Asp Tyr Leu Asp His Ser Asp Ile Ile Gly Trp Thr Arg Glu Gly Val 
                405                 410                 415     


Thr Glu Lys Pro Gly Ser Gly Leu Ala Ala Leu Ile Thr Asp Gly Pro 
            420                 425                 430         


Gly Gly Ser Lys Trp Met Tyr Val Gly Lys Gln His Ala Gly Lys Val 
        435                 440                 445             


Phe Tyr Asp Leu Thr Gly Asn Arg Ser Asp Thr Val Thr Ile Asn Ser 
    450                 455                 460                 


Asp Gly Trp Gly Glu Phe Lys Val Asn Gly Gly Ser Val Ser Val Trp 
465                 470                 475                 480 


Val Pro Arg Lys Thr Thr Val Ser Thr Ile Ala Arg Pro Ile Thr Thr 
                485                 490                 495     


Arg Pro Trp Thr Gly Glu Phe Val Arg Trp Thr Glu Pro Arg Leu Val 
            500                 505                 510         


Ala Trp Pro 
        515 


<210>  2
<211>  296
<212>  PRT
<213>  Talaromyces leycettanus


<220>
<221>  SIGNAL
<222>  (1)..(19)

<220>
<221>  mat_peptide
<222>  (20)..(296)

<400>  2

Met His Arg Pro Leu Gln Leu Trp Ala Leu Ala Ala Leu Thr Ser Leu 
                -15                 -10                 -5      


Val Thr Ala Ala Pro Ala Pro Val Leu Arg Arg Asp Val Ser Ser Ser 
        -1  1               5                   10              


Val Leu Ser Glu Leu Asp Leu Phe Ala Gln Tyr Ser Ala Ala Ala Tyr 
    15                  20                  25                  


Cys Ser Ser Asn Ile Gly Ser Pro Gly Thr Lys Leu Thr Cys Ser Val 
30                  35                  40                  45  


Gly Asn Cys Pro Arg Val Glu Ala Ala Asp Thr Glu Thr Leu Ile Glu 
                50                  55                  60      


Phe Asn Glu Ser Ser Ser Phe Gly Asp Val Thr Gly Tyr Ile Ala Val 
            65                  70                  75          


Asp Arg Thr Asn Ser Leu Leu Val Leu Ala Phe Arg Gly Ser Ser Thr 
        80                  85                  90              


Val Ser Asn Trp Glu Ala Asp Leu Asp Phe Pro Leu Thr Asp Ala Ser 
    95                  100                 105                 


Ser Leu Cys Ser Gly Cys Glu Ile His Ser Gly Phe Trp Ala Ala Trp 
110                 115                 120                 125 


Gln Thr Val Gln Ala Ser Ile Thr Ser Thr Leu Glu Ser Ala Ile Ala 
                130                 135                 140     


Ser Tyr Pro Gly Tyr Thr Leu Val Phe Thr Gly His Ser Tyr Gly Ala 
            145                 150                 155         


Ala Leu Ala Ala Ile Ala Ala Thr Thr Leu Arg Asn Ala Gly Tyr Thr 
        160                 165                 170             


Ile Gln Leu Tyr Asp Tyr Gly Gln Pro Arg Leu Gly Asn Leu Ala Leu 
    175                 180                 185                 


Ala Gln Tyr Ile Thr Ala Gln Thr Gln Gly Ala Asn Tyr Arg Val Thr 
190                 195                 200                 205 


His Thr Asp Asp Ile Val Pro Lys Leu Pro Pro Glu Leu Phe Gly Tyr 
                210                 215                 220     


His His Phe Ser Pro Glu Tyr Trp Ile Thr Ser Gly Asp Asn Val Thr 
            225                 230                 235         


Val Thr Thr Ser Asp Val Gln Val Val Thr Gly Ile Asp Ser Thr Ala 
        240                 245                 250             


Gly Asn Asp Gly Thr Leu Leu Asp Ser Thr Ser Ala His Asp Trp Tyr 
    255                 260                 265                 


Ile Val Tyr Ile Asp Gly Cys Asp 
270                 275         


<210>  3
<211>  583
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Hybrid protein sequence

<400>  3

Ala Thr Ser Asp Asp Trp Lys Gly Lys Ala Ile Tyr Gln Leu Leu Thr 
1               5                   10                  15      


Asp Arg Phe Gly Arg Ala Asp Asp Ser Thr Ser Asn Cys Ser Asn Leu 
            20                  25                  30          


Ser Asn Tyr Cys Gly Gly Thr Tyr Glu Gly Ile Thr Lys His Leu Asp 
        35                  40                  45              


Tyr Ile Ser Gly Met Gly Phe Asp Ala Ile Trp Ile Ser Pro Ile Pro 
    50                  55                  60                  


Lys Asn Ser Asp Gly Gly Tyr His Gly Tyr Trp Ala Thr Asp Phe Tyr 
65                  70                  75                  80  


Gln Leu Asn Ser Asn Phe Gly Asp Glu Ser Gln Leu Lys Ala Leu Ile 
                85                  90                  95      


Gln Ala Ala His Glu Arg Asp Met Tyr Val Met Leu Asp Val Val Ala 
            100                 105                 110         


Asn His Ala Gly Pro Thr Ser Asn Gly Tyr Ser Gly Tyr Thr Phe Gly 
        115                 120                 125             


Asp Ala Ser Leu Tyr His Pro Lys Cys Thr Ile Asp Tyr Asn Asp Gln 
    130                 135                 140                 


Thr Ser Ile Glu Gln Cys Trp Val Ala Asp Glu Leu Pro Asp Ile Asp 
145                 150                 155                 160 


Thr Glu Asn Ser Asp Asn Val Ala Ile Leu Asn Asp Ile Val Ser Gly 
                165                 170                 175     


Trp Val Gly Asn Tyr Ser Phe Asp Gly Ile Arg Ile Asp Thr Val Lys 
            180                 185                 190         


His Ile Arg Lys Asp Phe Trp Thr Gly Tyr Ala Glu Ala Ala Gly Val 
        195                 200                 205             


Phe Ala Thr Gly Glu Val Phe Asn Gly Asp Pro Ala Tyr Val Gly Pro 
    210                 215                 220                 


Tyr Gln Lys Tyr Leu Pro Ser Leu Ile Asn Tyr Pro Met Tyr Tyr Ala 
225                 230                 235                 240 


Leu Asn Asp Val Phe Val Ser Lys Ser Lys Gly Phe Ser Arg Ile Ser 
                245                 250                 255     


Glu Met Leu Gly Ser Asn Arg Asn Ala Phe Glu Asp Thr Ser Val Leu 
            260                 265                 270         


Thr Thr Phe Val Asp Asn His Asp Asn Pro Arg Phe Leu Asn Ser Gln 
        275                 280                 285             


Ser Asp Lys Ala Leu Phe Lys Asn Ala Leu Thr Tyr Val Leu Leu Gly 
    290                 295                 300                 


Glu Gly Ile Pro Ile Val Tyr Tyr Gly Ser Glu Gln Gly Phe Ser Gly 
305                 310                 315                 320 


Gly Ala Asp Pro Ala Asn Arg Glu Val Leu Trp Thr Thr Asn Tyr Asp 
                325                 330                 335     


Thr Ser Ser Asp Leu Tyr Gln Phe Ile Lys Thr Val Asn Ser Val Arg 
            340                 345                 350         


Met Lys Ser Asn Lys Ala Val Tyr Met Asp Ile Tyr Val Gly Asp Asn 
        355                 360                 365             


Ala Tyr Ala Phe Lys His Gly Asp Ala Leu Val Val Leu Asn Asn Tyr 
    370                 375                 380                 


Gly Ser Gly Ser Thr Asn Gln Val Ser Phe Ser Val Ser Gly Lys Phe 
385                 390                 395                 400 


Asp Ser Gly Ala Ser Leu Met Asp Ile Val Ser Asn Ile Thr Thr Thr 
                405                 410                 415     


Val Ser Ser Asp Gly Thr Val Thr Phe Asn Leu Lys Asp Gly Leu Pro 
            420                 425                 430         


Ala Ile Phe Thr Ser Ala Thr Gly Gly Thr Thr Thr Thr Ala Thr Pro 
        435                 440                 445             


Thr Gly Ser Gly Ser Val Thr Ser Thr Ser Lys Thr Thr Ala Thr Ala 
    450                 455                 460                 


Ser Lys Thr Ser Thr Ser Thr Ser Ser Thr Ser Cys Thr Thr Pro Thr 
465                 470                 475                 480 


Ala Val Ala Val Thr Phe Asp Leu Thr Ala Thr Thr Thr Tyr Gly Glu 
                485                 490                 495     


Asn Ile Tyr Leu Val Gly Ser Ile Ser Gln Leu Gly Asp Trp Glu Thr 
            500                 505                 510         


Ser Asp Gly Ile Ala Leu Ser Ala Asp Lys Tyr Thr Ser Ser Asp Pro 
        515                 520                 525             


Leu Trp Tyr Val Thr Val Thr Leu Pro Ala Gly Glu Ser Phe Glu Tyr 
    530                 535                 540                 


Lys Phe Ile Arg Ile Glu Ser Asp Asp Ser Val Glu Trp Glu Ser Asp 
545                 550                 555                 560 


Pro Asn Arg Glu Tyr Thr Val Pro Gln Ala Cys Gly Thr Ser Thr Ala 
                565                 570                 575     


Thr Val Thr Asp Thr Trp Arg 
            580             


<210>  4
<211>  412
<212>  PRT
<213>  Pyrococcus furiosus


<220>
<221>  mat_peptide
<222>  (1)..(412)
<223>  Pyrococcus furiosus protease (Pfu)

<400>  4

Ala Glu Leu Glu Gly Leu Asp Glu Ser Ala Ala Gln Val Met Ala Thr 
1               5                   10                  15      


Tyr Val Trp Asn Leu Gly Tyr Asp Gly Ser Gly Ile Thr Ile Gly Ile 
            20                  25                  30          


Ile Asp Thr Gly Ile Asp Ala Ser His Pro Asp Leu Gln Gly Lys Val 
        35                  40                  45              


Ile Gly Trp Val Asp Phe Val Asn Gly Arg Ser Tyr Pro Tyr Asp Asp 
    50                  55                  60                  


His Gly His Gly Thr His Val Ala Ser Ile Ala Ala Gly Thr Gly Ala 
65                  70                  75                  80  


Ala Ser Asn Gly Lys Tyr Lys Gly Met Ala Pro Gly Ala Lys Leu Ala 
                85                  90                  95      


Gly Ile Lys Val Leu Gly Ala Asp Gly Ser Gly Ser Ile Ser Thr Ile 
            100                 105                 110         


Ile Lys Gly Val Glu Trp Ala Val Asp Asn Lys Asp Lys Tyr Gly Ile 
        115                 120                 125             


Lys Val Ile Asn Leu Ser Leu Gly Ser Ser Gln Ser Ser Asp Gly Thr 
    130                 135                 140                 


Asp Ala Leu Ser Gln Ala Val Asn Ala Ala Trp Asp Ala Gly Leu Val 
145                 150                 155                 160 


Val Val Val Ala Ala Gly Asn Ser Gly Pro Asn Lys Tyr Thr Ile Gly 
                165                 170                 175     


Ser Pro Ala Ala Ala Ser Lys Val Ile Thr Val Gly Ala Val Asp Lys 
            180                 185                 190         


Tyr Asp Val Ile Thr Ser Phe Ser Ser Arg Gly Pro Thr Ala Asp Gly 
        195                 200                 205             


Arg Leu Lys Pro Glu Val Val Ala Pro Gly Asn Trp Ile Ile Ala Ala 
    210                 215                 220                 


Arg Ala Ser Gly Thr Ser Met Gly Gln Pro Ile Asn Asp Tyr Tyr Thr 
225                 230                 235                 240 


Ala Ala Pro Gly Thr Ser Met Ala Thr Pro His Val Ala Gly Ile Ala 
                245                 250                 255     


Ala Leu Leu Leu Gln Ala His Pro Ser Trp Thr Pro Asp Lys Val Lys 
            260                 265                 270         


Thr Ala Leu Ile Glu Thr Ala Asp Ile Val Lys Pro Asp Glu Ile Ala 
        275                 280                 285             


Asp Ile Ala Tyr Gly Ala Gly Arg Val Asn Ala Tyr Lys Ala Ile Asn 
    290                 295                 300                 


Tyr Asp Asn Tyr Ala Lys Leu Val Phe Thr Gly Tyr Val Ala Asn Lys 
305                 310                 315                 320 


Gly Ser Gln Thr His Gln Phe Val Ile Ser Gly Ala Ser Phe Val Thr 
                325                 330                 335     


Ala Thr Leu Tyr Trp Asp Asn Ala Asn Ser Asp Leu Asp Leu Tyr Leu 
            340                 345                 350         


Tyr Asp Pro Asn Gly Asn Gln Val Asp Tyr Ser Tyr Thr Ala Tyr Tyr 
        355                 360                 365             


Gly Phe Glu Lys Val Gly Tyr Tyr Asn Pro Thr Asp Gly Thr Trp Thr 
    370                 375                 380                 


Ile Lys Val Val Ser Tyr Ser Gly Ser Ala Asn Tyr Gln Val Asp Val 
385                 390                 395                 400 


Val Ser Asp Gly Ser Leu Ser Gln Pro Gly Ser Ser 
                405                 410         


<210>  5
<211>  573
<212>  PRT
<213>  Gloeophyllum sepiarium


<220>
<221>  SIGNAL
<222>  (1)..(17)

<220>
<221>  mat_peptide
<222>  (18)..(573)

<400>  5

Met Tyr Arg Phe Leu Val Cys Ala Leu Gly Leu Ala Ala Ser Val Leu 
        -15                 -10                 -5              


Ala Gln Ser Val Asp Ser Tyr Val Ser Ser Glu Gly Pro Ile Ala Lys 
-1  1               5                   10                  15  


Ala Gly Val Leu Ala Asn Ile Gly Pro Asn Gly Ser Lys Ala Ser Gly 
                20                  25                  30      


Ala Ser Ala Gly Val Val Val Ala Ser Pro Ser Thr Ser Asp Pro Asp 
            35                  40                  45          


Tyr Trp Tyr Thr Trp Thr Arg Asp Ser Ser Leu Val Phe Lys Ser Leu 
        50                  55                  60              


Ile Asp Gln Tyr Thr Thr Gly Ile Asp Ser Thr Ser Ser Leu Arg Thr 
    65                  70                  75                  


Leu Ile Asp Asp Phe Val Thr Ala Glu Ala Asn Leu Gln Gln Val Ser 
80                  85                  90                  95  


Asn Pro Ser Gly Thr Leu Thr Thr Gly Gly Leu Gly Glu Pro Lys Phe 
                100                 105                 110     


Asn Val Asp Glu Thr Ala Phe Thr Gly Ala Trp Gly Arg Pro Gln Arg 
            115                 120                 125         


Asp Gly Pro Ala Leu Arg Ser Thr Ala Leu Ile Thr Tyr Gly Asn Trp 
        130                 135                 140             


Leu Leu Ser Asn Gly Asn Thr Ser Tyr Val Thr Ser Asn Leu Trp Pro 
    145                 150                 155                 


Ile Ile Gln Asn Asp Leu Gly Tyr Val Val Ser Tyr Trp Asn Gln Ser 
160                 165                 170                 175 


Thr Tyr Asp Leu Trp Glu Glu Val Asp Ser Ser Ser Phe Phe Thr Thr 
                180                 185                 190     


Ala Val Gln His Arg Ala Leu Arg Glu Gly Ala Ala Phe Ala Thr Ala 
            195                 200                 205         


Ile Gly Gln Thr Ser Gln Val Ser Ser Tyr Thr Thr Gln Ala Asp Asn 
        210                 215                 220             


Leu Leu Cys Phe Leu Gln Ser Tyr Trp Asn Pro Ser Gly Gly Tyr Ile 
    225                 230                 235                 


Thr Ala Asn Thr Gly Gly Gly Arg Ser Gly Lys Asp Ala Asn Thr Leu 
240                 245                 250                 255 


Leu Ala Ser Ile His Thr Tyr Asp Pro Ser Ala Gly Cys Asp Ala Ala 
                260                 265                 270     


Thr Phe Gln Pro Cys Ser Asp Lys Ala Leu Ser Asn Leu Lys Val Tyr 
            275                 280                 285         


Val Asp Ser Phe Arg Ser Val Tyr Ser Ile Asn Ser Gly Val Ala Ser 
        290                 295                 300             


Asn Ala Ala Val Ala Thr Gly Arg Tyr Pro Glu Asp Ser Tyr Gln Gly 
    305                 310                 315                 


Gly Asn Pro Trp Tyr Leu Thr Thr Phe Ala Val Ala Glu Gln Leu Tyr 
320                 325                 330                 335 


Asp Ala Leu Asn Val Trp Glu Ser Gln Gly Ser Leu Glu Val Thr Ser 
                340                 345                 350     


Thr Ser Leu Ala Phe Phe Gln Gln Phe Ser Ser Gly Val Thr Ala Gly 
            355                 360                 365         


Thr Tyr Ser Ser Ser Ser Ser Thr Tyr Ser Thr Leu Thr Ser Ala Ile 
        370                 375                 380             


Lys Asn Phe Ala Asp Gly Phe Val Ala Ile Asn Ala Lys Tyr Thr Pro 
    385                 390                 395                 


Ser Asn Gly Gly Leu Ala Glu Gln Tyr Ser Lys Ser Asp Gly Ser Pro 
400                 405                 410                 415 


Leu Ser Ala Val Asp Leu Thr Trp Ser Tyr Ala Ser Ala Leu Thr Ala 
                420                 425                 430     


Phe Glu Ala Arg Asn Asn Thr Gln Phe Ala Gly Trp Gly Ala Ala Gly 
            435                 440                 445         


Leu Thr Val Pro Ser Ser Cys Ser Gly Asn Ser Gly Gly Pro Thr Val 
        450                 455                 460             


Ala Val Thr Phe Asn Val Asn Ala Glu Thr Val Trp Gly Glu Asn Ile 
    465                 470                 475                 


Tyr Leu Thr Gly Ser Val Asp Ala Leu Glu Asn Trp Ser Ala Asp Asn 
480                 485                 490                 495 


Ala Leu Leu Leu Ser Ser Ala Asn Tyr Pro Thr Trp Ser Ile Thr Val 
                500                 505                 510     


Asn Leu Pro Ala Ser Thr Ala Ile Glu Tyr Lys Tyr Ile Arg Lys Asn 
            515                 520                 525         


Asn Gly Ala Val Thr Trp Glu Ser Asp Pro Asn Asn Ser Ile Thr Thr 
        530                 535                 540             


Pro Ala Ser Gly Ser Thr Thr Glu Asn Asp Thr Trp Arg 
    545                 550                 555     


<210>  6
<211>  573
<212>  PRT
<213>  Pycnoporus sanguineus


<220>
<221>  SIGNAL
<222>  (1)..(18)

<220>
<221>  mat_peptide
<222>  (19)..(573)

<400>  6

Met Arg Phe Thr Leu Leu Ala Ser Leu Ile Gly Leu Ala Val Gly Ala 
            -15                 -10                 -5          


Phe Ala Gln Ser Ser Ala Val Asp Ala Tyr Val Ala Ser Glu Ser Pro 
    -1  1               5                   10                  


Ile Ala Lys Gln Gly Val Leu Asn Asn Ile Gly Pro Asn Gly Ser Lys 
15                  20                  25                  30  


Ala His Gly Ala Lys Ala Gly Ile Val Val Ala Ser Pro Ser Thr Glu 
                35                  40                  45      


Asn Pro Asp Tyr Leu Tyr Thr Trp Thr Arg Asp Ser Ser Leu Val Phe 
            50                  55                  60          


Lys Leu Leu Ile Asp Gln Phe Thr Ser Gly Asp Asp Thr Ser Leu Arg 
        65                  70                  75              


Gly Leu Ile Asp Asp Phe Thr Ser Ala Glu Ala Ile Leu Gln Gln Val 
    80                  85                  90                  


Ser Asn Pro Ser Gly Thr Val Ser Thr Gly Gly Leu Gly Glu Pro Lys 
95                  100                 105                 110 


Phe Asn Ile Asp Glu Thr Ala Phe Thr Gly Ala Trp Gly Arg Pro Gln 
                115                 120                 125     


Arg Asp Gly Pro Ala Leu Arg Ala Thr Ser Ile Ile Arg Tyr Ala Asn 
            130                 135                 140         


Trp Leu Leu Asp Asn Gly Asn Thr Thr Tyr Val Ser Asn Thr Leu Trp 
        145                 150                 155             


Pro Val Ile Gln Leu Asp Leu Asp Tyr Val Ala Asp Asn Trp Asn Gln 
    160                 165                 170                 


Ser Thr Phe Asp Leu Trp Glu Glu Val Asp Ser Ser Ser Phe Phe Thr 
175                 180                 185                 190 


Thr Ala Val Gln His Arg Ala Leu Arg Glu Gly Ala Thr Phe Ala Ser 
                195                 200                 205     


Arg Ile Gly Gln Ser Ser Val Val Ser Gly Tyr Thr Thr Gln Ala Asp 
            210                 215                 220         


Asn Leu Leu Cys Phe Leu Gln Ser Tyr Trp Asn Pro Ser Gly Gly Tyr 
        225                 230                 235             


Val Thr Ala Asn Thr Gly Gly Gly Arg Ser Gly Lys Asp Ser Asn Thr 
    240                 245                 250                 


Val Leu Thr Ser Ile His Thr Phe Asp Pro Ala Ala Gly Cys Asp Ala 
255                 260                 265                 270 


Ala Thr Phe Gln Pro Cys Ser Asp Lys Ala Leu Ser Asn Leu Lys Val 
                275                 280                 285     


Tyr Val Asp Ala Phe Arg Ser Ile Tyr Thr Ile Asn Asn Gly Ile Ala 
            290                 295                 300         


Ser Asn Ala Ala Val Ala Thr Gly Arg Tyr Pro Glu Asp Ser Tyr Met 
        305                 310                 315             


Gly Gly Asn Pro Trp Tyr Leu Thr Thr Ser Ala Val Ala Glu Gln Leu 
    320                 325                 330                 


Tyr Asp Ala Leu Tyr Val Trp Asp Gln Leu Gly Gly Leu Asn Val Thr 
335                 340                 345                 350 


Ser Thr Ser Leu Ala Phe Phe Gln Gln Phe Ala Ser Gly Leu Ser Thr 
                355                 360                 365     


Gly Thr Tyr Ser Ala Ser Ser Ser Thr Tyr Ala Thr Leu Thr Ser Ala 
            370                 375                 380         


Ile Arg Ser Phe Ala Asp Gly Phe Leu Ala Ile Asn Ala Lys Tyr Thr 
        385                 390                 395             


Pro Ala Asp Gly Gly Leu Ala Glu Gln Tyr Ser Arg Asn Asp Gly Thr 
    400                 405                 410                 


Pro Leu Ser Ala Val Asp Leu Thr Trp Ser Tyr Ala Ala Ala Leu Thr 
415                 420                 425                 430 


Ala Phe Ala Ala Arg Glu Gly Lys Thr Tyr Gly Ser Trp Gly Ala Ala 
                435                 440                 445     


Gly Leu Thr Val Pro Ala Ser Cys Ser Gly Gly Gly Gly Ala Thr Val 
            450                 455                 460         


Ala Val Thr Phe Asn Val Gln Ala Thr Thr Val Phe Gly Glu Asn Ile 
        465                 470                 475             


Tyr Ile Thr Gly Ser Val Ala Ala Leu Gln Asn Trp Ser Pro Asp Asn 
    480                 485                 490                 


Ala Leu Ile Leu Ser Ala Ala Asn Tyr Pro Thr Trp Ser Ile Thr Val 
495                 500                 505                 510 


Asn Leu Pro Ala Asn Thr Val Val Gln Tyr Lys Tyr Ile Arg Lys Phe 
                515                 520                 525     


Asn Gly Gln Val Thr Trp Glu Ser Asp Pro Asn Asn Gln Ile Thr Thr 
            530                 535                 540         


Pro Ser Gly Gly Ser Phe Thr Gln Asn Asp Val Trp Arg 
        545                 550                 555 


<210>  7
<211>  283
<212>  PRT
<213>  Bacillus thuringiensis


<220>
<221>  SIGNAL
<222>  (1)..(24)

<220>
<221>  mat_peptide
<222>  (25)..(282)

<400>  7

Met Lys Lys Lys Val Leu Ala Leu Ala Ala Ala Ile Thr Leu Val Ala 
                -20                 -15                 -10     


Pro Leu Gln Ser Val Ala Phe Ala His Glu Asn Asp Gly Gly Ser Lys 
            -5              -1  1               5               


Ile Lys Ile Val His Arg Trp Ser Ala Glu Asp Lys His Lys Glu Gly 
    10                  15                  20                  


Val Asn Ser His Leu Trp Ile Val Asn Arg Ala Ile Asp Ile Met Ser 
25                  30                  35                  40  


Arg Asn Thr Thr Leu Val Lys Gln Asp Arg Val Ala Gln Leu Asn Glu 
                45                  50                  55      


Trp Arg Thr Glu Leu Glu Asn Gly Ile Tyr Ala Ala Asp Tyr Glu Asn 
            60                  65                  70          


Pro Tyr Tyr Asp Asn Ser Thr Phe Ala Ser His Phe Tyr Asp Pro Asp 
        75                  80                  85              


Asn Gly Lys Thr Tyr Ile Pro Phe Ala Lys Gln Ala Lys Glu Thr Gly 
    90                  95                  100                 


Ala Lys Tyr Phe Lys Leu Ala Gly Glu Ser Tyr Lys Asn Lys Asp Met 
105                 110                 115                 120 


Lys Gln Ala Phe Phe Tyr Leu Gly Leu Ser Leu His Tyr Leu Gly Asp 
                125                 130                 135     


Val Asn Gln Pro Met His Ala Ala Asn Phe Thr Asn Leu Ser Tyr Pro 
            140                 145                 150         


Gln Gly Phe His Ser Lys Tyr Glu Asn Phe Val Asp Thr Ile Lys Asp 
        155                 160                 165             


Asn Tyr Lys Val Thr Asp Gly Asn Gly Tyr Trp Asn Trp Lys Gly Thr 
    170                 175                 180                 


Asn Pro Glu Asp Trp Ile His Gly Ala Ala Val Val Ala Lys Gln Asp 
185                 190                 195                 200 


Tyr Ser Gly Ile Val Asn Asp Asn Thr Lys Asp Trp Phe Val Lys Ala 
                205                 210                 215     


Ala Val Ser Gln Glu Tyr Ala Asp Lys Trp Arg Ala Glu Val Thr Pro 
            220                 225                 230         


Met Thr Gly Lys Arg Leu Met Asp Ala Gln Arg Val Thr Ala Gly Tyr 
        235                 240                 245             


Ile Gln Leu Trp Phe Asp Thr Tyr Gly Asp Arg 
    250                 255                 


<210>  8
<211>  274
<212>  PRT
<213>  Thermomyces lanuginosa

<400>  8

Glu Val Ser Gln Asp Leu Phe Asn Gln Phe Asn Leu Phe Ala Gln Tyr 
1               5                   10                  15      


Ser Ala Ala Ala Tyr Cys Gly Lys Asn Asn Asp Ala Pro Ala Gly Thr 
            20                  25                  30          


Asn Ile Thr Cys Thr Gly Asn Ala Cys Pro Glu Val Glu Lys Ala Asp 
        35                  40                  45              


Ala Thr Phe Leu Tyr Ser Phe Glu Asp Ser Gly Val Gly Asp Val Thr 
    50                  55                  60                  


Gly Phe Leu Ala Leu Asp Asn Thr Asn Lys Leu Ile Val Leu Ser Phe 
65                  70                  75                  80  


Arg Gly Ser Arg Ser Ile Glu Asn Trp Ile Ala Asn Leu Asn Phe Trp 
                85                  90                  95      


Leu Lys Lys Ile Asn Asp Ile Cys Ser Gly Cys Arg Gly His Asp Gly 
            100                 105                 110         


Phe Thr Ser Ser Trp Arg Ser Val Ala Asp Thr Leu Arg Gln Lys Val 
        115                 120                 125             


Glu Asp Ala Val Arg Glu His Pro Asp Tyr Arg Val Val Phe Thr Gly 
    130                 135                 140                 


His Ser Leu Gly Gly Ala Leu Ala Thr Val Ala Gly Ala Asp Leu Arg 
145                 150                 155                 160 


Gly Asn Gly Tyr Asp Ile Asp Val Phe Ser Tyr Gly Ala Pro Arg Val 
                165                 170                 175     


Gly Asn Arg Ala Phe Ala Glu Phe Leu Thr Val Gln Thr Gly Gly Thr 
            180                 185                 190         


Leu Tyr Arg Ile Thr His Thr Asn Asp Ile Val Pro Arg Leu Pro Pro 
        195                 200                 205             


Arg Glu Phe Gly Tyr Ser His Ser Ser Pro Glu Tyr Trp Ile Lys Ser 
    210                 215                 220                 


Gly Thr Leu Val Pro Val Thr Arg Asn Asp Ile Val Lys Ile Glu Gly 
225                 230                 235                 240 


Ile Asp Ala Thr Gly Gly Asn Asn Gln Pro Asn Ile Pro Asp Ile Pro 
                245                 250                 255     


Ala His Leu Trp Tyr Phe Gln Ala Thr Asp Ala Cys Asn Ala Gly Gly 
            260                 265                 270         


Phe Ser 
        


