                         SEQUENCE LISTING

<110>  SYNTHETIC GENOMICS INC

 
<120>  RECOMBINANT VIRUS REPLICON SYSTEMS AND USES THEREOF

<130>  SGI.011WO

<150>  62/409,228          
<151>  2016-10-17

<160>  24    

<170>  PatentIn version 3.5

<210>  1
<211>  8364
<212>  DNA
<213>  Artificial Sequence


<220>
<223>  Synthetic polynucleotide

<220>
<221>  misc_feature
<223>  Rep-Alpha replicon

<220>
<221>  misc_feature
<223>  T7 promoter, modified VEEV 5'UTR and nonstructural genes

<400>  1
taatacgact cactatagag aggcggcgca tgagagaagc ccagaccaat tacctaccca       60

aaatggagaa agttcacgtt gacatcgagg aagacagccc attcctcaga gctttgcagc      120

ggagcttccc gcagtttgag gtagaagcca agcaggtcac tgataatgac catgctaatg      180

ccagagcgtt ttcgcatctg gcttcaaaac tgatcgaaac ggaggtggac ccatccgaca      240

cgatccttga cattggaagt gcgcccgccc gcagaatgta ttctaagcac aagtatcatt      300

gtatctgtcc gatgagatgt gcggaagatc cggacagatt gtataagtat gcaactaagc      360

tgaagaaaaa ctgtaaggaa ataactgata aggaattgga caagaaaatg aaggagctcg      420

ccgccgtcat gagcgaccct gacctggaaa ctgagactat gtgcctccac gacgacgagt      480

cgtgtcgcta cgaagggcaa gtcgctgttt accaggatgt atacgcggtt gacggaccga      540

caagtctcta tcaccaagcc aataagggag ttagagtcgc ctactggata ggctttgaca      600

ccaccccttt tatgtttaag aacttggctg gagcatatcc atcatactct accaactggg      660

ccgacgaaac cgtgttaacg gctcgtaaca taggcctatg cagctctgac gttatggagc      720

ggtcacgtag agggatgtcc attcttagaa agaagtattt gaaaccatcc aacaatgttc      780

tattctctgt tggctcgacc atctaccacg agaagaggga cttactgagg agctggcacc      840

tgccgtctgt atttcactta cgtggcaagc aaaattacac atgtcggtgt gagactatag      900

ttagttgcga cgggtacgtc gttaaaagaa tagctatcag tccaggcctg tatgggaagc      960

cttcaggcta tgctgctacg atgcaccgcg agggattctt gtgctgcaaa gtgacagaca     1020

cattgaacgg ggagagggtc tcttttcccg tgtgcacgta tgtgccagct acattgtgtg     1080

accaaatgac tggcatactg gcaacagatg tcagtgcgga cgacgcgcaa aaactgctgg     1140

ttgggctcaa ccagcgtata gtcgtcaacg gtcgcaccca gagaaacacc aataccatga     1200

aaaattacct tttgcccgta gtggcccagg catttgctag gtgggcaaag gaatataagg     1260

aagatcaaga agatgaaagg ccactaggac tacgagatag acagttagtc atggggtgtt     1320

gttgggcttt tagaaggcac aagataacat ctatttataa gcgcccggat acccaaacca     1380

tcatcaaagt gaacagcgat ttccactcat tcgtgctgcc caggataggc agtaacacat     1440

tggagatcgg gctgagaaca agaatcagga aaatgttaga ggagcacaag gagccgtcac     1500

ctctcattac cgccgaggac gtacaagaag ctaagtgcgc agccgatgag gctaaggagg     1560

tgcgtgaagc cgaggagttg cgcgcagctc taccaccttt ggcagctgat gttgaggagc     1620

ccactctgga agccgatgtc gacttgatgt tacaagaggc tggggccggc tcagtggaga     1680

cacctcgtgg cttgataaag gttaccagct acgatggcga ggacaagatc ggctcttacg     1740

ctgtgctttc tccgcaggct gtactcaaga gtgaaaaatt atcttgcatc caccctctcg     1800

ctgaacaagt catagtgata acacactctg gccgaaaagg gcgttatgcc gtggaaccat     1860

accatggtaa agtagtggtg ccagagggac atgcaatacc cgtccaggac tttcaagctc     1920

tgagtgaaag tgccaccatt gtgtacaacg aacgtgagtt cgtaaacagg tacctgcacc     1980

atattgccac acatggagga gcgctgaaca ctgatgaaga atattacaaa actgtcaagc     2040

ccagcgagca cgacggcgaa tacctgtacg acatcgacag gaaacagtgc gtcaagaaag     2100

aactagtcac tgggctaggg ctcacaggcg agctggtgga tcctcccttc catgaattcg     2160

cctacgagag tctgagaaca cgaccagccg ctccttacca agtaccaacc ataggggtgt     2220

atggcgtgcc aggatcaggc aagtctggca tcattaaaag cgcagtcacc aaaaaagatc     2280

tagtggtgag cgccaagaaa gaaaactgtg cagaaattat aagggacgtc aagaaaatga     2340

aagggctgga cgtcaatgcc agaactgtgg actcagtgct cttgaatgga tgcaaacacc     2400

ccgtagagac cctgtatatt gacgaagctt ttgcttgtca tgcaggtact ctcagagcgc     2460

tcatagccat tataagacct aaaaaggcag tgctctgcgg ggatcccaaa cagtgcggtt     2520

tttttaacat gatgtgcctg aaagtgcatt ttaaccacga gatttgcaca caagtcttcc     2580

acaaaagcat ctctcgccgt tgcactaaat ctgtgacttc ggtcgtctca accttgtttt     2640

acgacaaaaa aatgagaacg acgaatccga aagagactaa gattgtgatt gacactaccg     2700

gcagtaccaa acctaagcag gacgatctca ttctcacttg tttcagaggg tgggtgaagc     2760

agttgcaaat agattacaaa ggcaacgaaa taatgacggc agctgcctct caagggctga     2820

cccgtaaagg tgtgtatgcc gttcggtaca aggtgaatga aaatcctctg tacgcaccca     2880

cctctgaaca tgtgaacgtc ctactgaccc gcacggagga ccgcatcgtg tggaaaacac     2940

tagccggcga cccatggata aaaacactga ctgccaagta ccctgggaat ttcactgcca     3000

cgatagagga gtggcaagca gagcatgatg ccatcatgag gcacatcttg gagagaccgg     3060

accctaccga cgtcttccag aataaggcaa acgtgtgttg ggccaaggct ttagtgccgg     3120

tgctgaagac cgctggcata gacatgacca ctgaacaatg gaacactgtg gattattttg     3180

aaacggacaa agctcactca gcagagatag tattgaacca actatgcgtg aggttctttg     3240

gactcgatct ggactccggt ctattttctg cacccactgt tccgttatcc attaggaata     3300

atcactggga taactccccg tcgcctaaca tgtacgggct gaataaagaa gtggtccgtc     3360

agctctctcg caggtaccca caactgcctc gggcagttgc cactggaaga gtctatgaca     3420

tgaacactgg tacactgcgc aattatgatc cgcgcataaa cctagtacct gtaaacagaa     3480

gactgcctca tgctttagtc ctccaccata atgaacaccc acagagtgac ttttcttcat     3540

tcgtcagcaa attgaagggc agaactgtcc tggtggtcgg ggaaaagttg tccgtcccag     3600

gcaaaatggt tgactggttg tcagaccggc ctgaggctac cttcagagct cggctggatt     3660

taggcatccc aggtgatgtg cccaaatatg acataatatt tgttaatgtg aggaccccat     3720

ataaatacca tcactatcag cagtgtgaag accatgccat taagcttagc atgttgacca     3780

agaaagcttg tctgcatctg aatcccggcg gaacctgtgt cagcataggt tatggttacg     3840

ctgacagggc cagcgaaagc atcattggtg ctatagcgcg gcagttcaag ttttcccggg     3900

tatgcaaacc gaaatcctca cttgaagaga cggaagttct gtttgtattc attgggtacg     3960

atcgcaaggc ccgtacgcac aatccttaca agctttcatc aaccttgacc aacatttata     4020

caggttccag actccacgaa gccggatgtg caccctcata tcatgtggtg cgaggggata     4080

ttgccacggc caccgaagga gtgattataa atgctgctaa cagcaaagga caacctggcg     4140

gaggggtgtg cggagcgctg tataagaaat tcccggaaag cttcgattta cagccgatcg     4200

aagtaggaaa agcgcgactg gtcaaaggtg cagctaaaca tatcattcat gccgtaggac     4260

caaacttcaa caaagtttcg gaggttgaag gtgacaaaca gttggcagag gcttatgagt     4320

ccatcgctaa gattgtcaac gataacaatt acaagtcagt agcgattcca ctgttgtcca     4380

ccggcatctt ttccgggaac aaagatcgac taacccaatc attgaaccat ttgctgacag     4440

ctttagacac cactgatgca gatgtagcca tatactgcag ggacaagaaa tgggaaatga     4500

ctctcaagga agcagtggct aggagagaag cagtggagga gatatgcata tccgacgact     4560

cttcagtgac agaacctgat gcagagctgg tgagggtgca tccgaagagt tctttggctg     4620

gaaggaaggg ctacagcaca agcgatggca aaactttctc atatttggaa gggaccaagt     4680

ttcaccaggc ggccaaggat atagcagaaa ttaatgccat gtggcccgtt gcaacggagg     4740

ccaatgagca ggtatgcatg tatatcctcg gagaaagcat gagcagtatt aggtcgaaat     4800

gccccgtcga agagtcggaa gcctccacac cacctagcac gctgccttgc ttgtgcatcc     4860

atgccatgac tccagaaaga gtacagcgcc taaaagcctc acgtccagaa caaattactg     4920

tgtgctcatc ctttccattg ccgaagtata gaatcactgg tgtgcagaag atccaatgct     4980

cccagcctat attgttctca ccgaaagtgc ctgcgtatat tcatccaagg aagtatctcg     5040

tggaaacacc accggtagac gagactccgg agccatcggc agagaaccaa tccacagagg     5100

ggacacctga acaaccacca cttataaccg aggatgagac caggactaga acgcctgagc     5160

cgatcatcat cgaagaggaa gaagaggata gcataagttt gctgtcagat ggcccgaccc     5220

accaggtgct gcaagtcgag gcagacattc acgggccgcc ctctgtatct agctcatcct     5280

ggtccattcc tcatgcatcc gactttgatg tggacagttt atccatactt gacaccctgg     5340

agggagctag cgtgaccagc ggggcaacgt cagccgagac taactcttac ttcgcaaaga     5400

gtatggagtt tctggcgcga ccggtgcctg cgcctcgaac agtattcagg aaccctccac     5460

atcccgctcc gcgcacaaga acaccgtcac ttgcacccag cagggcctgc tcgagaacca     5520

gcctagtttc caccccgcca ggcgtgaata gggtgatcac tagagaggag ctcgaggcgc     5580

ttaccccgtc acgcactcct agcaggtcgg tctcgagaac cagcctggtc tccaacccgc     5640

caggcgtaaa tagggtgatt acaagagagg agtttgaggc gttcgtagca caacaacaat     5700

gacggtttga tgcgggtgca tacatctttt cctccgacac cggtcaaggg catttacaac     5760

aaaaatcagt aaggcaaacg gtgctatccg aagtggtgtt ggagaggacc gaattggaga     5820

tttcgtatgc cccgcgcctc gaccaagaaa aagaagaatt actacgcaag aaattacagt     5880

taaatcccac acctgctaac agaagcagat accagtccag gaaggtggag aacatgaaag     5940

ccataacagc tagacgtatt ctgcaaggcc tagggcatta tttgaaggca gaaggaaaag     6000

tggagtgcta ccgaaccctg catcctgttc ctttgtattc atctagtgtg aaccgtgcct     6060

tttcaagccc caaggtcgca gtggaagcct gtaacgccat gttgaaagag aactttccga     6120

ctgtggcttc ttactgtatt attccagagt acgatgccta tttggacatg gttgacggag     6180

cttcatgctg cttagacact gccagttttt gccctgcaaa gctgcgcagc tttccaaaga     6240

aacactccta tttggaaccc acaatacgat cggcagtgcc ttcagcgatc cagaacacgc     6300

tccagaacgt cctggcagct gccacaaaaa gaaattgcaa tgtcacgcaa atgagagaat     6360

tgcccgtatt ggattcggcg gcctttaatg tggaatgctt caagaaatat gcgtgtaata     6420

atgaatattg ggaaacgttt aaagaaaacc ccatcaggct tactgaagaa aacgtggtaa     6480

attacattac caaattaaaa ggaccaaaag ctgctgctct ttttgcgaag acacataatt     6540

tgaatatgtt gcaggacata ccaatggaca ggtttgtaat ggacttaaag agagacgtga     6600

aagtgactcc aggaacaaaa catactgaag aacggcccaa ggtacaggtg atccaggctg     6660

ccgatccgct agcaacagcg tatctgtgcg gaatccaccg agagctggtt aggagattaa     6720

atgcggtcct gcttccgaac attcatacac tgtttgatat gtcggctgaa gactttgacg     6780

ctattatagc cgagcacttc cagcctgggg attgtgttct ggaaactgac atcgcgtcgt     6840

ttgataaaag tgaggacgac gccatggctc tgaccgcgtt aatgattctg gaagacttag     6900

gtgtggacgc agagctgttg acgctgattg aggcggcttt cggcgaaatt tcatcaatac     6960

atttgcccac taaaactaaa tttaaattcg gagccatgat gaaatctgga atgttcctca     7020

cactgtttgt gaacacagtc attaacattg taatcgcaag cagagtgttg agagaacggc     7080

taaccggatc accatgtgca gcattcattg gagatgacaa tatcgtgaaa ggagtcaaat     7140

cggacaaatt aatggcagac aggtgcgcca cctggttgaa tatggaagtc aagattatag     7200

atgctgtggt gggcgagaaa gcgccttatt tctgtggagg gtttattttg tgtgactccg     7260

tgaccggcac agcgtgccgt gtggcagacc ccctaaaaag gctgtttaag cttggcaaac     7320

ctctggcagc agacgatgaa catgatgatg acaggagaag ggcattgcat gaagagtcaa     7380

cacgctggaa ccgagtgggt attctttcag agctgtgcaa ggcagtagaa tcaaggtatg     7440

aaaccgtagg aacttccatc atagttatgg ccatgactac tctagctagc agtgttaaat     7500

cattcagcta cctgagaggg gcccctataa ctctctacgg ctaacctgaa tggactacga     7560

catagtctag tccgccaaga tatcatcgat acagcagcaa ttggcaagct gcttacatag     7620

aaggcgcgcc gtttaaacgg ccggccttaa ttaagtaacg atacagcagc aattggcaag     7680

ctgcttacat agaactcgcg gcgattggca tgccgcttta aaatttttat tttatttttc     7740

ttttcttttc cgaatcggat tttgttttta atatttcaaa aaaaaaaaaa aaaaaaaaaa     7800

aaaaaaaaaa aaaaaaaccc ctctctaaac ggaggggttt ttttcagcgt aactggactg     7860

gccacagtta ggcggccgcg catgttcatc atcagtaacc cgtatcgtga gcatcctctc     7920

tcgtttcatc ggtatcatta cctccatgaa cagaaatccc ccttacacgg aggcatcagt     7980

gaccaaacag gaaaaaaccg cccttaacat ggcccgcttt atcagaagcc agacattaac     8040

gcttctggag aaactcaacg agctggacgc ggatgaacag gcagacatct gtgaatcgct     8100

tcacgaccac gctgatgagc tttaccgcag ctgcctcgcg cgtttcggtg atgacggtga     8160

aaacctctga cacatgcagc tcccggagac ggtcacagct tgtctgtaag cggatgccgg     8220

gagcagacaa gcccgtcagg gcgcgtcagc gggtgttggc gggtgtcggg gcgcagccat     8280

gacccagtca cgtagcgata gcggagtgta tactggctta actatgcggc atcagagcag     8340

attgtactga gagtgcacca tatg                                            8364


<210>  2
<211>  50
<212>  DNA
<213>  Aura virus

<220>
<221>  misc_feature
<223>  5'UTR

<400>  2
atagcggacg gactagtact tgtactacag aattaactgc cgtgtgccgc                  50


<210>  3
<211>  50
<212>  DNA
<213>  Chikungunya virus

<220>
<221>  misc_feature
<223>  5'UTR

<400>  3
atggctgcgt gagacacacg tagcctacca gtttcttact gctctactct                  50


<210>  4
<211>  50
<212>  DNA
<213>  O'Nyong-Nyong virus

<220>
<221>  misc_feature
<223>  5'UTR

<400>  4
atagctgcgt gatacacaca cgcagcttac gggtttcata ctgctctact                  50


<210>  5
<211>  50
<212>  DNA
<213>  Bebaru virus

<220>
<221>  misc_feature
<223>  5'UTR

<400>  5
atggcggctg tgtgacacac gagccgtcga tttcaacctt cttgctccct                  50


<210>  6
<211>  50
<212>  DNA
<213>  Semliki Forest virus

<220>
<221>  misc_feature
<223>  5'UTR

<400>  6
atggcggatg tgtgacatac acgacgccaa aagattttgt tccagctcct                  50


<210>  7
<211>  50
<212>  DNA
<213>  Mayaro virus

<220>
<221>  misc_feature
<223>  5'UTR

<400>  7
atggcgggca agtgacactt gttccgccgg tcgtctctaa gctcttcctc                  50


<210>  8
<211>  50
<212>  DNA
<213>  Getah virus

<220>
<221>  misc_feature
<223>  5'UTR

<400>  8
atggcggacg tgtgacatca ccgttcgctc tttctaggat cctttgctac                  50


<210>  9
<211>  50
<212>  DNA
<213>  Sagiyama virus

<220>
<221>  misc_feature
<223>  5'UTR

<400>  9
atggcggacg tgtgacatca ccgttcgctc tttctaggat cctttgctac                  50


<210>  10
<211>  50
<212>  DNA
<213>  Ndumu virus

<220>
<221>  misc_feature
<223>  5'UTR

<400>  10
atggtgcgga gttgagagac gaagcaccaa acaactacgc ggctcaccat                  50


<210>  11
<211>  50
<212>  DNA
<213>  Middleburg virus

<220>
<221>  misc_feature
<223>  5'UTR

<400>  11
attggtggtt acgtacacgt gccaccaccc cccaccctcc aagcgatcca                  50


<210>  12
<211>  50
<212>  DNA
<213>  Eastern equine encephalitis virus

<220>
<221>  misc_feature
<223>  5'UTR

<400>  12
atagggtacg gtgtagaggc aaccacccta tttccaccta tccaaaatgg                  50


<210>  13
<211>  50
<212>  DNA
<213>  Fort Morgan virus

<220>
<221>  misc_feature
<223>  5'UTR

<400>  13
atagggtatg gtttagaggc gcctacccta cttaaccgat ccaaacatgg                  50


<210>  14
<211>  50
<212>  DNA
<213>  Buggy Creek virus

<220>
<221>  misc_feature
<223>  5'UTR

<400>  14
atagggtatg gtttagaggc gcctacccta cttaaccgat ccaaacatgg                  50


<210>  15
<211>  50
<212>  DNA
<213>  Venezuelan equine encephalitis virus

<220>
<221>  misc_feature
<223>  5'UTR

<400>  15
atgggcggcg caagagagaa gcccaaacca attacctacc caaaatggag                  50


<210>  16
<211>  50
<212>  DNA
<213>  Whataroa virus

<220>
<221>  misc_feature
<223>  5'UTR

<400>  16
attggcggca tagtacatac tatataaaag aaacagccga ccaattgcac                  50


<210>  17
<211>  50
<212>  DNA
<213>  Sindbis virus

<220>
<221>  misc_feature
<223>  5'UTR

<400>  17
attgacggcg tagtacacac tattgaatca aacagccgac caattgcact                  50


<210>  18
<211>  50
<212>  DNA
<213>  Bebaru virus

<220>
<221>  misc_feature
<223>  5'UTR

<400>  18
attggcggcg tagtacacac tattgaatca aacagccgac caattgcact                  50


<210>  19
<211>  9476
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Alpha-R-rFF-T2G

<220>
<221>  misc_feature
<222>  (1)..(18)
<223>  T7 promoter

<220>
<221>  misc_feature
<222>  (20)..(20)
<223>  T to G substitution

<220>
<221>  misc_feature
<222>  (9437)..(9476)
<223>  Poly A


<400>  19
taatacgact cactatagag aggcggcgca tgagagaagc ccagaccaat tacctaccca       60

aaatggagaa agttcacgtt gacatcgagg aagacagccc attcctcaga gctttgcagc      120

ggagcttccc gcagtttgag gtagaagcca agcaggtcac tgataatgac catgctaatg      180

ccagagcgtt ttcgcatctg gcttcaaaac tgatcgaaac ggaggtggac ccatccgaca      240

cgatccttga cattggaagt gcgcccgccc gcagaatgta ttctaagcac aagtatcatt      300

gtatctgtcc gatgagatgt gcggaagatc cggacagatt gtataagtat gcaactaagc      360

tgaagaaaaa ctgtaaggaa ataactgata aggaattgga caagaaaatg aaggagctcg      420

ccgccgtcat gagcgaccct gacctggaaa ctgagactat gtgcctccac gacgacgagt      480

cgtgtcgcta cgaagggcaa gtcgctgttt accaggatgt atacgcggtt gacggaccga      540

caagtctcta tcaccaagcc aataagggag ttagagtcgc ctactggata ggctttgaca      600

ccaccccttt tatgtttaag aacttggctg gagcatatcc atcatactct accaactggg      660

ccgacgaaac cgtgttaacg gctcgtaaca taggcctatg cagctctgac gttatggagc      720

ggtcacgtag agggatgtcc attcttagaa agaagtattt gaaaccatcc aacaatgttc      780

tattctctgt tggctcgacc atctaccacg agaagaggga cttactgagg agctggcacc      840

tgccgtctgt atttcactta cgtggcaagc aaaattacac atgtcggtgt gagactatag      900

ttagttgcga cgggtacgtc gttaaaagaa tagctatcag tccaggcctg tatgggaagc      960

cttcaggcta tgctgctacg atgcaccgcg agggattctt gtgctgcaaa gtgacagaca     1020

cattgaacgg ggagagggtc tcttttcccg tgtgcacgta tgtgccagct acattgtgtg     1080

accaaatgac tggcatactg gcaacagatg tcagtgcgga cgacgcgcaa aaactgctgg     1140

ttgggctcaa ccagcgtata gtcgtcaacg gtcgcaccca gagaaacacc aataccatga     1200

aaaattacct tttgcccgta gtggcccagg catttgctag gtgggcaaag gaatataagg     1260

aagatcaaga agatgaaagg ccactaggac tacgagatag acagttagtc atggggtgtt     1320

gttgggcttt tagaaggcac aagataacat ctatttataa gcgcccggat acccaaacca     1380

tcatcaaagt gaacagcgat ttccactcat tcgtgctgcc caggataggc agtaacacat     1440

tggagatcgg gctgagaaca agaatcagga aaatgttaga ggagcacaag gagccgtcac     1500

ctctcattac cgccgaggac gtacaagaag ctaagtgcgc agccgatgag gctaaggagg     1560

tgcgtgaagc cgaggagttg cgcgcagctc taccaccttt ggcagctgat gttgaggagc     1620

ccactctgga agccgatgtc gacttgatgt tacaagaggc tggggccggc tcagtggaga     1680

cacctcgtgg cttgataaag gttaccagct acgatggcga ggacaagatc ggctcttacg     1740

ctgtgctttc tccgcaggct gtactcaaga gtgaaaaatt atcttgcatc caccctctcg     1800

ctgaacaagt catagtgata acacactctg gccgaaaagg gcgttatgcc gtggaaccat     1860

accatggtaa agtagtggtg ccagagggac atgcaatacc cgtccaggac tttcaagctc     1920

tgagtgaaag tgccaccatt gtgtacaacg aacgtgagtt cgtaaacagg tacctgcacc     1980

atattgccac acatggagga gcgctgaaca ctgatgaaga atattacaaa actgtcaagc     2040

ccagcgagca cgacggcgaa tacctgtacg acatcgacag gaaacagtgc gtcaagaaag     2100

aactagtcac tgggctaggg ctcacaggcg agctggtgga tcctcccttc catgaattcg     2160

cctacgagag tctgagaaca cgaccagccg ctccttacca agtaccaacc ataggggtgt     2220

atggcgtgcc aggatcaggc aagtctggca tcattaaaag cgcagtcacc aaaaaagatc     2280

tagtggtgag cgccaagaaa gaaaactgtg cagaaattat aagggacgtc aagaaaatga     2340

aagggctgga cgtcaatgcc agaactgtgg actcagtgct cttgaatgga tgcaaacacc     2400

ccgtagagac cctgtatatt gacgaagctt ttgcttgtca tgcaggtact ctcagagcgc     2460

tcatagccat tataagacct aaaaaggcag tgctctgcgg ggatcccaaa cagtgcggtt     2520

tttttaacat gatgtgcctg aaagtgcatt ttaaccacga gatttgcaca caagtcttcc     2580

acaaaagcat ctctcgccgt tgcactaaat ctgtgacttc ggtcgtctca accttgtttt     2640

acgacaaaaa aatgagaacg acgaatccga aagagactaa gattgtgatt gacactaccg     2700

gcagtaccaa acctaagcag gacgatctca ttctcacttg tttcagaggg tgggtgaagc     2760

agttgcaaat agattacaaa ggcaacgaaa taatgacggc agctgcctct caagggctga     2820

cccgtaaagg tgtgtatgcc gttcggtaca aggtgaatga aaatcctctg tacgcaccca     2880

cctctgaaca tgtgaacgtc ctactgaccc gcacggagga ccgcatcgtg tggaaaacac     2940

tagccggcga cccatggata aaaacactga ctgccaagta ccctgggaat ttcactgcca     3000

cgatagagga gtggcaagca gagcatgatg ccatcatgag gcacatcttg gagagaccgg     3060

accctaccga cgtcttccag aataaggcaa acgtgtgttg ggccaaggct ttagtgccgg     3120

tgctgaagac cgctggcata gacatgacca ctgaacaatg gaacactgtg gattattttg     3180

aaacggacaa agctcactca gcagagatag tattgaacca actatgcgtg aggttctttg     3240

gactcgatct ggactccggt ctattttctg cacccactgt tccgttatcc attaggaata     3300

atcactggga taactccccg tcgcctaaca tgtacgggct gaataaagaa gtggtccgtc     3360

agctctctcg caggtaccca caactgcctc gggcagttgc cactggaaga gtctatgaca     3420

tgaacactgg tacactgcgc aattatgatc cgcgcataaa cctagtacct gtaaacagaa     3480

gactgcctca tgctttagtc ctccaccata atgaacaccc acagagtgac ttttcttcat     3540

tcgtcagcaa attgaagggc agaactgtcc tggtggtcgg ggaaaagttg tccgtcccag     3600

gcaaaatggt tgactggttg tcagaccggc ctgaggctac cttcagagct cggctggatt     3660

taggcatccc aggtgatgtg cccaaatatg acataatatt tgttaatgtg aggaccccat     3720

ataaatacca tcactatcag cagtgtgaag accatgccat taagcttagc atgttgacca     3780

agaaagcttg tctgcatctg aatcccggcg gaacctgtgt cagcataggt tatggttacg     3840

ctgacagggc cagcgaaagc atcattggtg ctatagcgcg gcagttcaag ttttcccggg     3900

tatgcaaacc gaaatcctca cttgaagaga cggaagttct gtttgtattc attgggtacg     3960

atcgcaaggc ccgtacgcac aatccttaca agctttcatc aaccttgacc aacatttata     4020

caggttccag actccacgaa gccggatgtg caccctcata tcatgtggtg cgaggggata     4080

ttgccacggc caccgaagga gtgattataa atgctgctaa cagcaaagga caacctggcg     4140

gaggggtgtg cggagcgctg tataagaaat tcccggaaag cttcgattta cagccgatcg     4200

aagtaggaaa agcgcgactg gtcaaaggtg cagctaaaca tatcattcat gccgtaggac     4260

caaacttcaa caaagtttcg gaggttgaag gtgacaaaca gttggcagag gcttatgagt     4320

ccatcgctaa gattgtcaac gataacaatt acaagtcagt agcgattcca ctgttgtcca     4380

ccggcatctt ttccgggaac aaagatcgac taacccaatc attgaaccat ttgctgacag     4440

ctttagacac cactgatgca gatgtagcca tatactgcag ggacaagaaa tgggaaatga     4500

ctctcaagga agcagtggct aggagagaag cagtggagga gatatgcata tccgacgact     4560

cttcagtgac agaacctgat gcagagctgg tgagggtgca tccgaagagt tctttggctg     4620

gaaggaaggg ctacagcaca agcgatggca aaactttctc atatttggaa gggaccaagt     4680

ttcaccaggc ggccaaggat atagcagaaa ttaatgccat gtggcccgtt gcaacggagg     4740

ccaatgagca ggtatgcatg tatatcctcg gagaaagcat gagcagtatt aggtcgaaat     4800

gccccgtcga agagtcggaa gcctccacac cacctagcac gctgccttgc ttgtgcatcc     4860

atgccatgac tccagaaaga gtacagcgcc taaaagcctc acgtccagaa caaattactg     4920

tgtgctcatc ctttccattg ccgaagtata gaatcactgg tgtgcagaag atccaatgct     4980

cccagcctat attgttctca ccgaaagtgc ctgcgtatat tcatccaagg aagtatctcg     5040

tggaaacacc accggtagac gagactccgg agccatcggc agagaaccaa tccacagagg     5100

ggacacctga acaaccacca cttataaccg aggatgagac caggactaga acgcctgagc     5160

cgatcatcat cgaagaggaa gaagaggata gcataagttt gctgtcagat ggcccgaccc     5220

accaggtgct gcaagtcgag gcagacattc acgggccgcc ctctgtatct agctcatcct     5280

ggtccattcc tcatgcatcc gactttgatg tggacagttt atccatactt gacaccctgg     5340

agggagctag cgtgaccagc ggggcaacgt cagccgagac taactcttac ttcgcaaaga     5400

gtatggagtt tctggcgcga ccggtgcctg cgcctcgaac agtattcagg aaccctccac     5460

atcccgctcc gcgcacaaga acaccgtcac ttgcacccag cagggcctgc tcgagaacca     5520

gcctagtttc caccccgcca ggcgtgaata gggtgatcac tagagaggag ctcgaggcgc     5580

ttaccccgtc acgcactcct agcaggtcgg tctcgagaac cagcctggtc tccaacccgc     5640

caggcgtaaa tagggtgatt acaagagagg agtttgaggc gttcgtagca caacaacaat     5700

gacggtttga tgcgggtgca tacatctttt cctccgacac cggtcaaggg catttacaac     5760

aaaaatcagt aaggcaaacg gtgctatccg aagtggtgtt ggagaggacc gaattggaga     5820

tttcgtatgc cccgcgcctc gaccaagaaa aagaagaatt actacgcaag aaattacagt     5880

taaatcccac acctgctaac agaagcagat accagtccag gaaggtggag aacatgaaag     5940

ccataacagc tagacgtatt ctgcaaggcc tagggcatta tttgaaggca gaaggaaaag     6000

tggagtgcta ccgaaccctg catcctgttc ctttgtattc atctagtgtg aaccgtgcct     6060

tttcaagccc caaggtcgca gtggaagcct gtaacgccat gttgaaagag aactttccga     6120

ctgtggcttc ttactgtatt attccagagt acgatgccta tttggacatg gttgacggag     6180

cttcatgctg cttagacact gccagttttt gccctgcaaa gctgcgcagc tttccaaaga     6240

aacactccta tttggaaccc acaatacgat cggcagtgcc ttcagcgatc cagaacacgc     6300

tccagaacgt cctggcagct gccacaaaaa gaaattgcaa tgtcacgcaa atgagagaat     6360

tgcccgtatt ggattcggcg gcctttaatg tggaatgctt caagaaatat gcgtgtaata     6420

atgaatattg ggaaacgttt aaagaaaacc ccatcaggct tactgaagaa aacgtggtaa     6480

attacattac caaattaaaa ggaccaaaag ctgctgctct ttttgcgaag acacataatt     6540

tgaatatgtt gcaggacata ccaatggaca ggtttgtaat ggacttaaag agagacgtga     6600

aagtgactcc aggaacaaaa catactgaag aacggcccaa ggtacaggtg atccaggctg     6660

ccgatccgct agcaacagcg tatctgtgcg gaatccaccg agagctggtt aggagattaa     6720

atgcggtcct gcttccgaac attcatacac tgtttgatat gtcggctgaa gactttgacg     6780

ctattatagc cgagcacttc cagcctgggg attgtgttct ggaaactgac atcgcgtcgt     6840

ttgataaaag tgaggacgac gccatggctc tgaccgcgtt aatgattctg gaagacttag     6900

gtgtggacgc agagctgttg acgctgattg aggcggcttt cggcgaaatt tcatcaatac     6960

atttgcccac taaaactaaa tttaaattcg gagccatgat gaaatctgga atgttcctca     7020

cactgtttgt gaacacagtc attaacattg taatcgcaag cagagtgttg agagaacggc     7080

taaccggatc accatgtgca gcattcattg gagatgacaa tatcgtgaaa ggagtcaaat     7140

cggacaaatt aatggcagac aggtgcgcca cctggttgaa tatggaagtc aagattatag     7200

atgctgtggt gggcgagaaa gcgccttatt tctgtggagg gtttattttg tgtgactccg     7260

tgaccggcac agcgtgccgt gtggcagacc ccctaaaaag gctgtttaag cttggcaaac     7320

ctctggcagc agacgatgaa catgatgatg acaggagaag ggcattgcat gaagagtcaa     7380

cacgctggaa ccgagtgggt attctttcag agctgtgcaa ggcagtagaa tcaaggtatg     7440

aaaccgtagg aacttccatc atagttatgg ccatgactac tctagctagc agtgttaaat     7500

cattcagcta cctgagaggg gcccctataa ctctctacgg ctaacctgaa tggactacga     7560

catagtctag tccgccaaga tatcgcacca tggaaaatat ggaaaacgac gagaacatcg     7620

tggtgggccc caagcccttc taccccatcg aggaaggcag cgccggcacc cagctgcgga     7680

agtacatgga aagatacgcc aagctgggcg ccattgcctt caccaacgcc gtgaccggcg     7740

tggactacag ctacgccgag tacctggaaa agagctgctg cctgggcaag gctctgcaga     7800

actacggcct ggtggtggac ggccggatcg ccctgtgcag cgagaactgc gaggaattct     7860

tcatccccgt gatcgccggc ctgttcatcg gcgtgggcgt ggctcccacc aacgagatct     7920

acaccctgcg ggagctggtg cacagcctgg gcatcagcaa gcccaccatc gtgttcagca     7980

gcaagaaggg cctggacaaa gtcatcaccg tgcagaaaac cgtgaccacc atcaagacca     8040

tcgtgatcct ggacagcaag gtggactacc ggggctacca gtgcctggac accttcatca     8100

agcggaacac cccccctggc ttccaggcca gcagcttcaa gaccgtggag gtggaccgga     8160

aagaacaggt ggccctgatc atgaacagca gcggcagcac cggcctgccc aagggcgtgc     8220

agctgaccca cgagaacacc gtgacccggt tcagccacgc cagggacccc atctacggca     8280

accaggtgtc ccccggcacc gccgtgctga ccgtggtgcc cttccaccac ggcttcggca     8340

tgttcaccac cctgggctac ctgatctgcg gcttccgggt ggtgatgctg accaagttcg     8400

acgaggaaac cttcctgaaa accctgcagg actacaagtg cacctacgtg attctggtgc     8460

ccaccctgtt cgccatcctg aacaagagcg agctgctgaa caagtacgac ctgagcaacc     8520

tggtggagat cgccagcggc ggagcccccc tgagcaaaga agtgggagag gccgtcgcca     8580

ggcggttcaa tctgcccggc gtgcggcagg gctacggcct gaccgagaca accagcgcca     8640

tcatcatcac ccccgagggc gacgacaagc ctggagccag cggcaaggtg gtgcccctgt     8700

tcaaggccaa agtgatcgac ctggacacca agaagagcct gggccccaac agacggggcg     8760

aagtgtgcgt gaagggcccc atgctgatga agggctacgt gaacaacccc gaggccacca     8820

aagagctgat cgacgaagag ggctggctgc acaccggcga catcggctac tacgacgaag     8880

agaagcactt cttcatcgtg gaccggctga agagcctgat caagtacaag ggctatcagg     8940

tgccccctgc cgagctggaa agcgtcctgc tgcagcaccc cagcatcttc gacgccggcg     9000

tggccggggt gccagatcct gtggccggcg agctgcctgg cgccgtggtg gtgctggaat     9060

ccggcaagaa catgaccgag aaagaagtga tggactacgt cgccagccag gtgtccaacg     9120

ccaagcggct gagaggcggc gtgagattcg tggacgaagt gccaaagggc ctgaccggca     9180

agatcgacgg cagggccatc cgggagatcc tgaagaaacc cgtggccaag atgtgattaa     9240

ttgatcgata cagcagcaat tggcaagctg cttacataga aggcgcgccg tttaaacggc     9300

cggccttaat taagtaacga tacagcagca attggcaagc tgcttacata gaactcgcgg     9360

cgattggcat gccgctttaa aatttttatt ttatttttct tttcttttcc gaatcggatt     9420

ttgtttttaa tatttcaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaa         9476


<210>  20
<211>  1647
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic polynucleotide

<220>
<221>  misc_feature
<223>  Red Firefly reporter gene - rFF

<400>  20
atggaaaata tggaaaacga cgagaacatc gtggtgggcc ccaagccctt ctaccccatc       60

gaggaaggca gcgccggcac ccagctgcgg aagtacatgg aaagatacgc caagctgggc      120

gccattgcct tcaccaacgc cgtgaccggc gtggactaca gctacgccga gtacctggaa      180

aagagctgct gcctgggcaa ggctctgcag aactacggcc tggtggtgga cggccggatc      240

gccctgtgca gcgagaactg cgaggaattc ttcatccccg tgatcgccgg cctgttcatc      300

ggcgtgggcg tggctcccac caacgagatc tacaccctgc gggagctggt gcacagcctg      360

ggcatcagca agcccaccat cgtgttcagc agcaagaagg gcctggacaa agtcatcacc      420

gtgcagaaaa ccgtgaccac catcaagacc atcgtgatcc tggacagcaa ggtggactac      480

cggggctacc agtgcctgga caccttcatc aagcggaaca ccccccctgg cttccaggcc      540

agcagcttca agaccgtgga ggtggaccgg aaagaacagg tggccctgat catgaacagc      600

agcggcagca ccggcctgcc caagggcgtg cagctgaccc acgagaacac cgtgacccgg      660

ttcagccacg ccagggaccc catctacggc aaccaggtgt cccccggcac cgccgtgctg      720

accgtggtgc ccttccacca cggcttcggc atgttcacca ccctgggcta cctgatctgc      780

ggcttccggg tggtgatgct gaccaagttc gacgaggaaa ccttcctgaa aaccctgcag      840

gactacaagt gcacctacgt gattctggtg cccaccctgt tcgccatcct gaacaagagc      900

gagctgctga acaagtacga cctgagcaac ctggtggaga tcgccagcgg cggagccccc      960

ctgagcaaag aagtgggaga ggccgtcgcc aggcggttca atctgcccgg cgtgcggcag     1020

ggctacggcc tgaccgagac aaccagcgcc atcatcatca cccccgaggg cgacgacaag     1080

cctggagcca gcggcaaggt ggtgcccctg ttcaaggcca aagtgatcga cctggacacc     1140

aagaagagcc tgggccccaa cagacggggc gaagtgtgcg tgaagggccc catgctgatg     1200

aagggctacg tgaacaaccc cgaggccacc aaagagctga tcgacgaaga gggctggctg     1260

cacaccggcg acatcggcta ctacgacgaa gagaagcact tcttcatcgt ggaccggctg     1320

aagagcctga tcaagtacaa gggctatcag gtgccccctg ccgagctgga aagcgtcctg     1380

ctgcagcacc ccagcatctt cgacgccggc gtggccgggg tgccagatcc tgtggccggc     1440

gagctgcctg gcgccgtggt ggtgctggaa tccggcaaga acatgaccga gaaagaagtg     1500

atggactacg tcgccagcca ggtgtccaac gccaagcggc tgagaggcgg cgtgagattc     1560

gtggacgaag tgccaaaggg cctgaccggc aagatcgacg gcagggccat ccgggagatc     1620

ctgaagaaac ccgtggccaa gatgtga                                         1647


<210>  21
<211>  729
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic polynucleotide

<220>
<221>  misc_feature
<223>  EGFP

<400>  21
atgggaagag ccggcgtgag caagggcgag gagctgttca ccggggtggt gcccatcctg       60

gtcgagctgg acggcgacgt aaacggccac aagttcagcg tgtccggcga gggcgagggc      120

gatgccacct acggcaagct gaccctgaag ctgatctgca ccaccggcaa gctgcccgtg      180

ccctggccca ccctcgtgac caccctgggc tacggcctgc agtgcttcgc ccgctacccc      240

gaccacatga agcagcacga cttcttcaag tccgccatgc ccgaaggcta cgtccaggag      300

cgcaccatct tcttcaagga cgacggcaac tacaagaccc gcgccgaggt gaagttcgag      360

ggcgacaccc tggtgaaccg catcgagctg aagggcatcg acttcaagga ggacggcaac      420

atcctggggc acaagctgga gtacaactac aacagccaca acgtctatat caccgccgac      480

aagcagaaga acggcatcaa ggccaacttc aagatccgcc acaacatcga ggacggcggc      540

gtgcagctcg ccgaccacta ccagcagaac acccccatcg gcgacggccc cgtgctgctg      600

cccgacaacc actacctgag ctaccagtcc gccctgagca aagaccccaa cgagaagcgc      660

gatcacatgg tcctgctgga gttcgtgacc gccgccggga tcactctcgg catggacgag      720

ctgtacaag                                                              729


<210>  22
<211>  1707
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic polynucleotide

<220>
<221>  misc_feature
<223>  HA Influenza A virus
       A-VietNam-1203-2004 (H5N1) - GenBank 

<400>  22
atggagaaaa tagtgcttct ttttgcaata gtcagtcttg ttaaaagtga tcagatttgc       60

attggttacc atgcaaacaa ctcgacagag caggttgaca caataatgga aaagaacgtt      120

actgttacac atgcccaaga catactggaa aagaaacaca acgggaagct ctgcgatcta      180

gatggagtga agcctctaat tttgagagat tgtagcgtag ctggatggct cctcggaaac      240

ccaatgtgtg acgaattcat caatgtgccg gaatggtctt acatagtgga gaaggccaat      300

ccagtcaatg acctctgtta cccaggggat ttcaatgact atgaagaatt gaaacaccta      360

ttgagcagaa taaaccattt tgagaaaatt cagatcatcc ccaaaagttc ttggtccagt      420

catgaagcct cattaggggt gagctcagca tgtccatacc agggaaagtc ctcctttttc      480

agaaatgtgg tatggcttat caaaaagaac agtacatacc caacaataaa gaggagctac      540

aataatacca accaagaaga tcttttggta ctgtggggga ttcaccatcc taatgatgcg      600

gcagagcaga caaagctcta tcaaaaccca accacctata tttccgttgg gacatcaaca      660

ctaaaccaga gattggtacc aagaatagct actagatcca aagtaaacgg gcaaagtgga      720

aggatggagt tcttctggac aattttaaag ccgaatgatg caatcaactt cgagagtaat      780

ggaaatttca ttgctccaga atatgcatac aaaattgtca agaaagggga ctcaacaatt      840

atgaaaagtg aattggaata tggtaactgc aacaccaagt gtcaaactcc aatgggggcg      900

ataaactcta gcatgccatt ccacaatata caccctctca ccattgggga atgccccaaa      960

tatgtgaaat caaacagatt agtccttgcg actgggctca gaaatagccc tcaaagagag     1020

agaagaagaa aaaagagagg attatttgga gctatagcag gttttataga gggaggatgg     1080

cagggaatgg tagatggttg gtatgggtac caccatagca atgagcaggg gagtgggtac     1140

gctgcagaca aagaatccac tcaaaaggca atagatggag tcaccaataa ggtcaactcg     1200

atcattgaca aaatgaacac tcagtttgag gccgttggaa gggaatttaa caacttagaa     1260

aggagaatag agaatttaaa caagaagatg gaagacgggt tcctagatgt ctggacttat     1320

aatgctgaac ttctggttct catggaaaat gagagaactc tagactttca tgactcaaat     1380

gtcaagaacc tttacgacaa ggtccgacta cagcttaggg ataatgcaaa ggagctgggt     1440

aacggttgtt tcgagttcta tcataaatgt gataatgaat gtatggaaag tgtaagaaat     1500

ggaacgtatg actacccgca gtattcagaa gaagcgagac taaaaagaga ggaaataagt     1560

ggagtaaaat tggaatcaat aggaatttac caaatactgt caatttattc tacagtggcg     1620

agttccctag cactggcaat catggtagct ggtctatcct tatggatgtg ctccaatggg     1680

tcgttacaat gcagaatttg catttaa                                         1707


<210>  23
<211>  29
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic polynucleotide

<220>
<223>  VEE 5' T-G nt 2 F - Forward Primer

<400>  23
cgactcacta tagagaggcg gcgcatgag                                         29


<210>  24
<211>  29
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic polynucleotide

<220>
<223>  VEE 5' T-G nt 2 R - Reverse Primer

<400>  24
ctcatgcgcc gcctctctat agtgagtcg                                         29


