SEQUENCE LISTING

<110> Cellectis

<120> TAL- effector nuclease (TALEN) - MODIFIED ALLOGENIC CELLS SUITABLE FOR THERAPY

<130> P81704129PCT00

<150> US62/410,187
<151> 2016-10-19

<150> PA201770240
<151> 2017-03-31

<160> 23

<170> PatentIn version 3.5

<210>  1

<211>  2781
<212>  DNA
<213>  artificial sequence

<220>
<223>  TALEN TRAC_T01-L1

<400>  1
atgggcgatc ctaaaaagaa acgtaaggtc atcgatatcg ccgatctacg cacgctcggc     60
tacagccagc agcaacagga gaagatcaaa ccgaaggttc gttcgacagt ggcgcagcac    120
cacgaggcac tggtcggcca cgggtttaca cacgcgcaca tcgttgcgtt aagccaacac    180
ccggcagcgt tagggaccgt cgctgtcaag tatcaggaca tgatcgcagc gttgccagag    240
gcgacacacg aagcgatcgt tggcgtcggc aaacagtggt ccggcgcacg cgctctggag    300
gccttgctca cggtggcggg agagttgaga ggtccaccgt tacagttgga cacaggccaa    360
cttctcaaga ttgcaaaacg tggcggcgtg accgcagtgg aggcagtgca tgcatggcgc    420
aatgcactga cgggtgcccc gctcaacttg accccccagc aggtggtggc catcgccagc    480
aatggcggtg gcaagcaggc gctggagacg gtccagcggc tgttgccggt gctgtgccag    540
gcccacggct tgacccccca gcaggtggtg gccatcgcca gcaataatgg tggcaagcag    600
gcgctggaga cggtccagcg gctgttgccg gtgctgtgcc aggcccacgg cttgaccccc    660
cagcaggtgg tggccatcgc cagcaatggc ggtggcaagc aggcgctgga gacggtccag    720
cggctgttgc cggtgctgtg ccaggcccac ggcttgaccc cggagcaggt ggtggccatc    780
gccagccacg atggcggcaa gcaggcgctg gagacggtcc agcggctgtt gccggtgctg    840
tgccaggccc acggcttgac cccggagcag gtggtggcca tcgccagcca cgatggcggc    900
aagcaggcgc tggagacggt ccagcggctg ttgccggtgc tgtgccaggc ccacggcttg    960
accccggagc aggtggtggc catcgccagc cacgatggcg gcaagcaggc gctggagacg   1020
gtccagcggc tgttgccggt gctgtgccag gcccacggct tgaccccgga gcaggtggtg   1080
gccatcgcca gcaatattgg tggcaagcag gcgctggaga cggtgcaggc gctgttgccg   1140
gtgctgtgcc aggcccacgg cttgaccccg gagcaggtgg tggccatcgc cagccacgat   1200
ggcggcaagc aggcgctgga gacggtccag cggctgttgc cggtgctgtg ccaggcccac   1260
ggcttgaccc cggagcaggt ggtggccatc gccagcaata ttggtggcaa gcaggcgctg   1320
gagacggtgc aggcgctgtt gccggtgctg tgccaggccc acggcttgac cccccagcag   1380
gtggtggcca tcgccagcaa taatggtggc aagcaggcgc tggagacggt ccagcggctg   1440
ttgccggtgc tgtgccaggc ccacggcttg accccggagc aggtggtggc catcgccagc   1500
aatattggtg gcaagcaggc gctggagacg gtgcaggcgc tgttgccggt gctgtgccag   1560
gcccacggct tgacccccca gcaggtggtg gccatcgcca gcaatggcgg tggcaagcag   1620
gcgctggaga cggtccagcg gctgttgccg gtgctgtgcc aggcccacgg cttgaccccg   1680
gagcaggtgg tggccatcgc cagcaatatt ggtggcaagc aggcgctgga gacggtgcag   1740
gcgctgttgc cggtgctgtg ccaggcccac ggcttgaccc cccagcaggt ggtggccatc   1800
gccagcaatg gcggtggcaa gcaggcgctg gagacggtcc agcggctgtt gccggtgctg   1860
tgccaggccc acggcttgac cccggagcag gtggtggcca tcgccagcca cgatggcggc   1920
aagcaggcgc tggagacggt ccagcggctg ttgccggtgc tgtgccaggc ccacggcttg   1980
acccctcagc aggtggtggc catcgccagc aatggcggcg gcaggccggc gctggagagc   2040
attgttgccc agttatctcg ccctgatccg gcgttggccg cgttgaccaa cgaccacctc   2100
gtcgccttgg cctgcctcgg cgggcgtcct gcgctggatg cagtgaaaaa gggattgggg   2160
gatcctatca gccgttccca gctggtgaag tccgagctgg aggagaagaa atccgagttg   2220
aggcacaagc tgaagtacgt gccccacgag tacatcgagc tgatcgagat cgcccggaac   2280
agcacccagg accgtatcct ggagatgaag gtgatggagt tcttcatgaa ggtgtacggc   2340
tacaggggca agcacctggg cggctccagg aagcccgacg gcgccatcta caccgtgggc   2400
tcccccatcg actacggcgt gatcgtggac accaaggcct actccggcgg ctacaacctg   2460
cccatcggcc aggccgacga aatgcagagg tacgtggagg agaaccagac caggaacaag   2520
cacatcaacc ccaacgagtg gtggaaggtg tacccctcca gcgtgaccga gttcaagttc   2580
ctgttcgtgt ccggccactt caagggcaac tacaaggccc agctgaccag gctgaaccac   2640
atcaccaact gcaacggcgc cgtgctgtcc gtggaggagc tcctgatcgg cggcgagatg   2700
atcaaggccg gcaccctgac cctggaggag gtgaggagga agttcaacaa cggcgagatc   2760
aacttcgcgg ccgactgata a                                             2781


<210>  2

<211>  2781
<212>  DNA
<213>  artificial sequence

<220>
<223>  TALEN TRAC_T01-R1_

<400>  2
atgggcgatc ctaaaaagaa acgtaaggtc atcgatatcg ccgatctacg cacgctcggc     60
tacagccagc agcaacagga gaagatcaaa ccgaaggttc gttcgacagt ggcgcagcac    120
cacgaggcac tggtcggcca cgggtttaca cacgcgcaca tcgttgcgtt aagccaacac    180
ccggcagcgt tagggaccgt cgctgtcaag tatcaggaca tgatcgcagc gttgccagag    240
gcgacacacg aagcgatcgt tggcgtcggc aaacagtggt ccggcgcacg cgctctggag    300
gccttgctca cggtggcggg agagttgaga ggtccaccgt tacagttgga cacaggccaa    360
cttctcaaga ttgcaaaacg tggcggcgtg accgcagtgg aggcagtgca tgcatggcgc    420
aatgcactga cgggtgcccc gctcaacttg accccggagc aggtggtggc catcgccagc    480
cacgatggcg gcaagcaggc gctggagacg gtccagcggc tgttgccggt gctgtgccag    540
gcccacggct tgacccccca gcaggtggtg gccatcgcca gcaatggcgg tggcaagcag    600
gcgctggaga cggtccagcg gctgttgccg gtgctgtgcc aggcccacgg cttgaccccg    660
gagcaggtgg tggccatcgc cagccacgat ggcggcaagc aggcgctgga gacggtccag    720
cggctgttgc cggtgctgtg ccaggcccac ggcttgaccc cggagcaggt ggtggccatc    780
gccagcaata ttggtggcaa gcaggcgctg gagacggtgc aggcgctgtt gccggtgctg    840
tgccaggccc acggcttgac cccccagcag gtggtggcca tcgccagcaa taatggtggc    900
aagcaggcgc tggagacggt ccagcggctg ttgccggtgc tgtgccaggc ccacggcttg    960
accccggagc aggtggtggc catcgccagc cacgatggcg gcaagcaggc gctggagacg   1020
gtccagcggc tgttgccggt gctgtgccag gcccacggct tgacccccca gcaggtggtg   1080
gccatcgcca gcaatggcgg tggcaagcag gcgctggaga cggtccagcg gctgttgccg   1140
gtgctgtgcc aggcccacgg cttgaccccc cagcaggtgg tggccatcgc cagcaataat   1200
ggtggcaagc aggcgctgga gacggtccag cggctgttgc cggtgctgtg ccaggcccac   1260
ggcttgaccc cccagcaggt ggtggccatc gccagcaata atggtggcaa gcaggcgctg   1320
gagacggtcc agcggctgtt gccggtgctg tgccaggccc acggcttgac cccccagcag   1380
gtggtggcca tcgccagcaa tggcggtggc aagcaggcgc tggagacggt ccagcggctg   1440
ttgccggtgc tgtgccaggc ccacggcttg accccggagc aggtggtggc catcgccagc   1500
aatattggtg gcaagcaggc gctggagacg gtgcaggcgc tgttgccggt gctgtgccag   1560
gcccacggct tgaccccgga gcaggtggtg gccatcgcca gccacgatgg cggcaagcag   1620
gcgctggaga cggtccagcg gctgttgccg gtgctgtgcc aggcccacgg cttgaccccg   1680
gagcaggtgg tggccatcgc cagcaatatt ggtggcaagc aggcgctgga gacggtgcag   1740
gcgctgttgc cggtgctgtg ccaggcccac ggcttgaccc cggagcaggt ggtggccatc   1800
gccagccacg atggcggcaa gcaggcgctg gagacggtcc agcggctgtt gccggtgctg   1860
tgccaggccc acggcttgac cccccagcag gtggtggcca tcgccagcaa taatggtggc   1920
aagcaggcgc tggagacggt ccagcggctg ttgccggtgc tgtgccaggc ccacggcttg   1980
acccctcagc aggtggtggc catcgccagc aatggcggcg gcaggccggc gctggagagc   2040
attgttgccc agttatctcg ccctgatccg gcgttggccg cgttgaccaa cgaccacctc   2100
gtcgccttgg cctgcctcgg cgggcgtcct gcgctggatg cagtgaaaaa gggattgggg   2160
gatcctatca gccgttccca gctggtgaag tccgagctgg aggagaagaa atccgagttg   2220
aggcacaagc tgaagtacgt gccccacgag tacatcgagc tgatcgagat cgcccggaac   2280
agcacccagg accgtatcct ggagatgaag gtgatggagt tcttcatgaa ggtgtacggc   2340
tacaggggca agcacctggg cggctccagg aagcccgacg gcgccatcta caccgtgggc   2400
tcccccatcg actacggcgt gatcgtggac accaaggcct actccggcgg ctacaacctg   2460
cccatcggcc aggccgacga aatgcagagg tacgtggagg agaaccagac caggaacaag   2520
cacatcaacc ccaacgagtg gtggaaggtg tacccctcca gcgtgaccga gttcaagttc   2580
ctgttcgtgt ccggccactt caagggcaac tacaaggccc agctgaccag gctgaaccac   2640
atcaccaact gcaacggcgc cgtgctgtcc gtggaggagc tcctgatcgg cggcgagatg   2700
atcaaggccg gcaccctgac cctggaggag gtgaggagga agttcaacaa cggcgagatc   2760
aacttcgcgg ccgactgata a                                             2781


<210>  3

<211>  925
<212>  PRT
<213>  artificial sequence

<220>
<223>  TALEN  TRAC_T01-L1

<400>  3
Met Gly Asp Pro Lys Lys Lys Arg Lys Val Ile Asp Ile Ala Asp Leu 
1               5                   10                  15      


Arg Thr Leu Gly Tyr Ser Gln Gln Gln Gln Glu Lys Ile Lys Pro Lys 
            20                  25                  30          


Val Arg Ser Thr Val Ala Gln His His Glu Ala Leu Val Gly His Gly 
        35                  40                  45              


Phe Thr His Ala His Ile Val Ala Leu Ser Gln His Pro Ala Ala Leu 
    50                  55                  60                  


Gly Thr Val Ala Val Lys Tyr Gln Asp Met Ile Ala Ala Leu Pro Glu 
65                  70                  75                  80  


Ala Thr His Glu Ala Ile Val Gly Val Gly Lys Gln Trp Ser Gly Ala 
                85                  90                  95      


Arg Ala Leu Glu Ala Leu Leu Thr Val Ala Gly Glu Leu Arg Gly Pro 
            100                 105                 110         


Pro Leu Gln Leu Asp Thr Gly Gln Leu Leu Lys Ile Ala Lys Arg Gly 
        115                 120                 125             


Gly Val Thr Ala Val Glu Ala Val His Ala Trp Arg Asn Ala Leu Thr 
    130                 135                 140                 


Gly Ala Pro Leu Asn Leu Thr Pro Gln Gln Val Val Ala Ile Ala Ser 
145                 150                 155                 160 


Asn Gly Gly Gly Lys Gln Ala Leu Glu Thr Val Gln Arg Leu Leu Pro 
                165                 170                 175     


Val Leu Cys Gln Ala His Gly Leu Thr Pro Gln Gln Val Val Ala Ile 
            180                 185                 190         


Ala Ser Asn Asn Gly Gly Lys Gln Ala Leu Glu Thr Val Gln Arg Leu 
        195                 200                 205             


Leu Pro Val Leu Cys Gln Ala His Gly Leu Thr Pro Gln Gln Val Val 
    210                 215                 220                 


Ala Ile Ala Ser Asn Gly Gly Gly Lys Gln Ala Leu Glu Thr Val Gln 
225                 230                 235                 240 


Arg Leu Leu Pro Val Leu Cys Gln Ala His Gly Leu Thr Pro Glu Gln 
                245                 250                 255     


Val Val Ala Ile Ala Ser His Asp Gly Gly Lys Gln Ala Leu Glu Thr 
            260                 265                 270         


Val Gln Arg Leu Leu Pro Val Leu Cys Gln Ala His Gly Leu Thr Pro 
        275                 280                 285             


Glu Gln Val Val Ala Ile Ala Ser His Asp Gly Gly Lys Gln Ala Leu 
    290                 295                 300                 


Glu Thr Val Gln Arg Leu Leu Pro Val Leu Cys Gln Ala His Gly Leu 
305                 310                 315                 320 


Thr Pro Glu Gln Val Val Ala Ile Ala Ser His Asp Gly Gly Lys Gln 
                325                 330                 335     


Ala Leu Glu Thr Val Gln Arg Leu Leu Pro Val Leu Cys Gln Ala His 
            340                 345                 350         


Gly Leu Thr Pro Glu Gln Val Val Ala Ile Ala Ser Asn Ile Gly Gly 
        355                 360                 365             


Lys Gln Ala Leu Glu Thr Val Gln Ala Leu Leu Pro Val Leu Cys Gln 
    370                 375                 380                 


Ala His Gly Leu Thr Pro Glu Gln Val Val Ala Ile Ala Ser His Asp 
385                 390                 395                 400 


Gly Gly Lys Gln Ala Leu Glu Thr Val Gln Arg Leu Leu Pro Val Leu 
                405                 410                 415     


Cys Gln Ala His Gly Leu Thr Pro Glu Gln Val Val Ala Ile Ala Ser 
            420                 425                 430         


Asn Ile Gly Gly Lys Gln Ala Leu Glu Thr Val Gln Ala Leu Leu Pro 
        435                 440                 445             


Val Leu Cys Gln Ala His Gly Leu Thr Pro Gln Gln Val Val Ala Ile 
    450                 455                 460                 


Ala Ser Asn Asn Gly Gly Lys Gln Ala Leu Glu Thr Val Gln Arg Leu 
465                 470                 475                 480 


Leu Pro Val Leu Cys Gln Ala His Gly Leu Thr Pro Glu Gln Val Val 
                485                 490                 495     


Ala Ile Ala Ser Asn Ile Gly Gly Lys Gln Ala Leu Glu Thr Val Gln 
            500                 505                 510         


Ala Leu Leu Pro Val Leu Cys Gln Ala His Gly Leu Thr Pro Gln Gln 
        515                 520                 525             


Val Val Ala Ile Ala Ser Asn Gly Gly Gly Lys Gln Ala Leu Glu Thr 
    530                 535                 540                 


Val Gln Arg Leu Leu Pro Val Leu Cys Gln Ala His Gly Leu Thr Pro 
545                 550                 555                 560 


Glu Gln Val Val Ala Ile Ala Ser Asn Ile Gly Gly Lys Gln Ala Leu 
                565                 570                 575     


Glu Thr Val Gln Ala Leu Leu Pro Val Leu Cys Gln Ala His Gly Leu 
            580                 585                 590         


Thr Pro Gln Gln Val Val Ala Ile Ala Ser Asn Gly Gly Gly Lys Gln 
        595                 600                 605             


Ala Leu Glu Thr Val Gln Arg Leu Leu Pro Val Leu Cys Gln Ala His 
    610                 615                 620                 


Gly Leu Thr Pro Glu Gln Val Val Ala Ile Ala Ser His Asp Gly Gly 
625                 630                 635                 640 


Lys Gln Ala Leu Glu Thr Val Gln Arg Leu Leu Pro Val Leu Cys Gln 
                645                 650                 655     


Ala His Gly Leu Thr Pro Gln Gln Val Val Ala Ile Ala Ser Asn Gly 
            660                 665                 670         


Gly Gly Arg Pro Ala Leu Glu Ser Ile Val Ala Gln Leu Ser Arg Pro 
        675                 680                 685             


Asp Pro Ala Leu Ala Ala Leu Thr Asn Asp His Leu Val Ala Leu Ala 
    690                 695                 700                 


Cys Leu Gly Gly Arg Pro Ala Leu Asp Ala Val Lys Lys Gly Leu Gly 
705                 710                 715                 720 


Asp Pro Ile Ser Arg Ser Gln Leu Val Lys Ser Glu Leu Glu Glu Lys 
                725                 730                 735     


Lys Ser Glu Leu Arg His Lys Leu Lys Tyr Val Pro His Glu Tyr Ile 
            740                 745                 750         


Glu Leu Ile Glu Ile Ala Arg Asn Ser Thr Gln Asp Arg Ile Leu Glu 
        755                 760                 765             


Met Lys Val Met Glu Phe Phe Met Lys Val Tyr Gly Tyr Arg Gly Lys 
    770                 775                 780                 


His Leu Gly Gly Ser Arg Lys Pro Asp Gly Ala Ile Tyr Thr Val Gly 
785                 790                 795                 800 


Ser Pro Ile Asp Tyr Gly Val Ile Val Asp Thr Lys Ala Tyr Ser Gly 
                805                 810                 815     


Gly Tyr Asn Leu Pro Ile Gly Gln Ala Asp Glu Met Gln Arg Tyr Val 
            820                 825                 830         


Glu Glu Asn Gln Thr Arg Asn Lys His Ile Asn Pro Asn Glu Trp Trp 
        835                 840                 845             


Lys Val Tyr Pro Ser Ser Val Thr Glu Phe Lys Phe Leu Phe Val Ser 
    850                 855                 860                 


Gly His Phe Lys Gly Asn Tyr Lys Ala Gln Leu Thr Arg Leu Asn His 
865                 870                 875                 880 


Ile Thr Asn Cys Asn Gly Ala Val Leu Ser Val Glu Glu Leu Leu Ile 
                885                 890                 895     


Gly Gly Glu Met Ile Lys Ala Gly Thr Leu Thr Leu Glu Glu Val Arg 
            900                 905                 910         


Arg Lys Phe Asn Asn Gly Glu Ile Asn Phe Ala Ala Asp 
        915                 920                 925 


<210>  4

<211>  925
<212>  PRT
<213>  artificial sequence

<220>
<223>  TALEN  TRAC_T01-R1

<400>  4
Met Gly Asp Pro Lys Lys Lys Arg Lys Val Ile Asp Ile Ala Asp Leu 
1               5                   10                  15      


Arg Thr Leu Gly Tyr Ser Gln Gln Gln Gln Glu Lys Ile Lys Pro Lys 
            20                  25                  30          


Val Arg Ser Thr Val Ala Gln His His Glu Ala Leu Val Gly His Gly 
        35                  40                  45              


Phe Thr His Ala His Ile Val Ala Leu Ser Gln His Pro Ala Ala Leu 
    50                  55                  60                  


Gly Thr Val Ala Val Lys Tyr Gln Asp Met Ile Ala Ala Leu Pro Glu 
65                  70                  75                  80  


Ala Thr His Glu Ala Ile Val Gly Val Gly Lys Gln Trp Ser Gly Ala 
                85                  90                  95      


Arg Ala Leu Glu Ala Leu Leu Thr Val Ala Gly Glu Leu Arg Gly Pro 
            100                 105                 110         


Pro Leu Gln Leu Asp Thr Gly Gln Leu Leu Lys Ile Ala Lys Arg Gly 
        115                 120                 125             


Gly Val Thr Ala Val Glu Ala Val His Ala Trp Arg Asn Ala Leu Thr 
    130                 135                 140                 


Gly Ala Pro Leu Asn Leu Thr Pro Glu Gln Val Val Ala Ile Ala Ser 
145                 150                 155                 160 


His Asp Gly Gly Lys Gln Ala Leu Glu Thr Val Gln Arg Leu Leu Pro 
                165                 170                 175     


Val Leu Cys Gln Ala His Gly Leu Thr Pro Gln Gln Val Val Ala Ile 
            180                 185                 190         


Ala Ser Asn Gly Gly Gly Lys Gln Ala Leu Glu Thr Val Gln Arg Leu 
        195                 200                 205             


Leu Pro Val Leu Cys Gln Ala His Gly Leu Thr Pro Glu Gln Val Val 
    210                 215                 220                 


Ala Ile Ala Ser His Asp Gly Gly Lys Gln Ala Leu Glu Thr Val Gln 
225                 230                 235                 240 


Arg Leu Leu Pro Val Leu Cys Gln Ala His Gly Leu Thr Pro Glu Gln 
                245                 250                 255     


Val Val Ala Ile Ala Ser Asn Ile Gly Gly Lys Gln Ala Leu Glu Thr 
            260                 265                 270         


Val Gln Ala Leu Leu Pro Val Leu Cys Gln Ala His Gly Leu Thr Pro 
        275                 280                 285             


Gln Gln Val Val Ala Ile Ala Ser Asn Asn Gly Gly Lys Gln Ala Leu 
    290                 295                 300                 


Glu Thr Val Gln Arg Leu Leu Pro Val Leu Cys Gln Ala His Gly Leu 
305                 310                 315                 320 


Thr Pro Glu Gln Val Val Ala Ile Ala Ser His Asp Gly Gly Lys Gln 
                325                 330                 335     


Ala Leu Glu Thr Val Gln Arg Leu Leu Pro Val Leu Cys Gln Ala His 
            340                 345                 350         


Gly Leu Thr Pro Gln Gln Val Val Ala Ile Ala Ser Asn Gly Gly Gly 
        355                 360                 365             


Lys Gln Ala Leu Glu Thr Val Gln Arg Leu Leu Pro Val Leu Cys Gln 
    370                 375                 380                 


Ala His Gly Leu Thr Pro Gln Gln Val Val Ala Ile Ala Ser Asn Asn 
385                 390                 395                 400 


Gly Gly Lys Gln Ala Leu Glu Thr Val Gln Arg Leu Leu Pro Val Leu 
                405                 410                 415     


Cys Gln Ala His Gly Leu Thr Pro Gln Gln Val Val Ala Ile Ala Ser 
            420                 425                 430         


Asn Asn Gly Gly Lys Gln Ala Leu Glu Thr Val Gln Arg Leu Leu Pro 
        435                 440                 445             


Val Leu Cys Gln Ala His Gly Leu Thr Pro Gln Gln Val Val Ala Ile 
    450                 455                 460                 


Ala Ser Asn Gly Gly Gly Lys Gln Ala Leu Glu Thr Val Gln Arg Leu 
465                 470                 475                 480 


Leu Pro Val Leu Cys Gln Ala His Gly Leu Thr Pro Glu Gln Val Val 
                485                 490                 495     


Ala Ile Ala Ser Asn Ile Gly Gly Lys Gln Ala Leu Glu Thr Val Gln 
            500                 505                 510         


Ala Leu Leu Pro Val Leu Cys Gln Ala His Gly Leu Thr Pro Glu Gln 
        515                 520                 525             


Val Val Ala Ile Ala Ser His Asp Gly Gly Lys Gln Ala Leu Glu Thr 
    530                 535                 540                 


Val Gln Arg Leu Leu Pro Val Leu Cys Gln Ala His Gly Leu Thr Pro 
545                 550                 555                 560 


Glu Gln Val Val Ala Ile Ala Ser Asn Ile Gly Gly Lys Gln Ala Leu 
                565                 570                 575     


Glu Thr Val Gln Ala Leu Leu Pro Val Leu Cys Gln Ala His Gly Leu 
            580                 585                 590         


Thr Pro Glu Gln Val Val Ala Ile Ala Ser His Asp Gly Gly Lys Gln 
        595                 600                 605             


Ala Leu Glu Thr Val Gln Arg Leu Leu Pro Val Leu Cys Gln Ala His 
    610                 615                 620                 


Gly Leu Thr Pro Gln Gln Val Val Ala Ile Ala Ser Asn Asn Gly Gly 
625                 630                 635                 640 


Lys Gln Ala Leu Glu Thr Val Gln Arg Leu Leu Pro Val Leu Cys Gln 
                645                 650                 655     


Ala His Gly Leu Thr Pro Gln Gln Val Val Ala Ile Ala Ser Asn Gly 
            660                 665                 670         


Gly Gly Arg Pro Ala Leu Glu Ser Ile Val Ala Gln Leu Ser Arg Pro 
        675                 680                 685             


Asp Pro Ala Leu Ala Ala Leu Thr Asn Asp His Leu Val Ala Leu Ala 
    690                 695                 700                 


Cys Leu Gly Gly Arg Pro Ala Leu Asp Ala Val Lys Lys Gly Leu Gly 
705                 710                 715                 720 


Asp Pro Ile Ser Arg Ser Gln Leu Val Lys Ser Glu Leu Glu Glu Lys 
                725                 730                 735     


Lys Ser Glu Leu Arg His Lys Leu Lys Tyr Val Pro His Glu Tyr Ile 
            740                 745                 750         


Glu Leu Ile Glu Ile Ala Arg Asn Ser Thr Gln Asp Arg Ile Leu Glu 
        755                 760                 765             


Met Lys Val Met Glu Phe Phe Met Lys Val Tyr Gly Tyr Arg Gly Lys 
    770                 775                 780                 


His Leu Gly Gly Ser Arg Lys Pro Asp Gly Ala Ile Tyr Thr Val Gly 
785                 790                 795                 800 


Ser Pro Ile Asp Tyr Gly Val Ile Val Asp Thr Lys Ala Tyr Ser Gly 
                805                 810                 815     


Gly Tyr Asn Leu Pro Ile Gly Gln Ala Asp Glu Met Gln Arg Tyr Val 
            820                 825                 830         


Glu Glu Asn Gln Thr Arg Asn Lys His Ile Asn Pro Asn Glu Trp Trp 
        835                 840                 845             


Lys Val Tyr Pro Ser Ser Val Thr Glu Phe Lys Phe Leu Phe Val Ser 
    850                 855                 860                 


Gly His Phe Lys Gly Asn Tyr Lys Ala Gln Leu Thr Arg Leu Asn His 
865                 870                 875                 880 


Ile Thr Asn Cys Asn Gly Ala Val Leu Ser Val Glu Glu Leu Leu Ile 
                885                 890                 895     


Gly Gly Glu Met Ile Lys Ala Gly Thr Leu Thr Leu Glu Glu Val Arg 
            900                 905                 910         


Arg Lys Phe Asn Asn Gly Glu Ile Asn Phe Ala Ala Asp 
        915                 920                 925 


<210>  5

<211>  22
<212>  DNA
<213>  artificial sequence

<220>
<223>  FORWARD

<400>  5
gctggggttt tgaagaagat cc                                              22


<210>  6

<211>  19
<212>  DNA
<213>  artificial sequence

<220>
<223>  REVERSE

<400>  6
gacttcctct gccctcacc                                                  19


<210>  7

<211>  20
<212>  DNA
<213>  artificial sequence

<220>
<223>  PROBE

<400>  7
cccttgtcca tcactggcat                                                 20


<210>  8

<211>  411
<212>  DNA
<213>  artificial sequence

<220>
<223>  SEQUENCE IN TRAC

<400>  8
gctggggttt tgaagaagat cctattaaat aaaagaataa gcagtattat taagtagccc     60
tgcatttcag gtttccttga gtggcaggcc aggcctggcc gtgaacgttc actgaaatca    120
tggcctcttg gccaagattg atagcttgtg cctgtccctg agtcccagtc catcacgagc    180
agctggtttc taagatgcta tttcccgtat aaagcatgag accgtgactt gccagcccca    240
cagagccccg cccttgtcca tcactggcat ctggactcca gcctgggttg gggcaaagag    300
ggaaatgaga tcatgtccta accctgatcc tcttgtccca cagatatcca gtccggtgag    360
ggcagaggaa gtcttctaac atgcggtgac gtggaggaga atccgggccc c             411


<210>  9

<211>  2658
<212>  DNA
<213>  artificial sequence

<220>
<223>  SEQUENCE IN TRAC ANTI-CD22 CAR

<400>  9
aagtagccct gcatttcagg tttccttgag tggcaggcca ggcctggccg tgaacgttca     60
ctgaaatcat ggcctcttgg ccaagattga tagcttgtgc ctgtccctga gtcccagtcc    120
atcacgagca gctggtttct aagatgctat ttcccgtata aagcatgaga ccgtgacttg    180
ccagccccac agagccccgc ccttgtccat cactggcatc tggactccag cctgggttgg    240
ggcaaagagg gaaatgagat catgtcctaa ccctgatcct cttgtcccac agatatccag    300
tccggtgagg gcagaggaag tcttctaaca tgcggtgacg tggaggagaa tccgggcccc    360
ggatccgctc tgcccgtcac cgctctgctg ctgccactgg ccctgctgct gcacgcagca    420
agaccaggag ggggaggcag ctgcccctac agcaacccca gcctgtgcag cggaggcggc    480
ggcagcggcg gagggggtag ccaggtgcag ctgcagcaga gcggccctgg cctggtgaag    540
ccaagccaga cactgtccct gacctgcgcc atcagcggcg attccgtgag ctccaactcc    600
gccgcctgga attggatcag gcagtcccct tctcggggcc tggagtggct gggaaggaca    660
tactatcggt ctaagtggta caacgattat gccgtgtctg tgaagagcag aatcacaatc    720
aaccctgaca cctccaagaa tcagttctct ctgcagctga atagcgtgac accagaggac    780
accgccgtgt actattgcgc cagggaggtg accggcgacc tggaggatgc ctttgacatc    840
tggggccagg gcacaatggt gaccgtgtct agcggaggag gaggatccgg aggaggagga    900
tctggcggcg gcggcagcga tatccagatg acacagtccc catcctctct gagcgcctcc    960
gtgggcgaca gagtgacaat cacctgtagg gcctcccaga ccatctggtc ttacctgaac   1020
tggtatcagc agaggcccgg caaggcccct aatctgctga tctacgcagc aagctccctg   1080
cagagcggag tgccatccag attctctggc aggggctccg gcacagactt caccctgacc   1140
atctctagcc tgcaggccga ggacttcgcc acctactatt gccagcagtc ttatagcatc   1200
ccccagacat ttggccaggg caccaagctg gagatcaagg gaagcggagg gggaggcagc   1260
tgcccctaca gcaaccccag cctgtgcagc ggaggcggcg gcagcgagct gcccacccag   1320
ggcaccttct ccaacgtgtc caccaacgtg agcccagcca agcccaccac caccgcctgt   1380
ccttattcca atccttccct gtgtgctccc accacaaccc cagcaccaag gccacctaca   1440
cctgcaccaa ccatcgcctc tcagcccctg agcctgagac ctgaggcatg taggccagca   1500
gcaggaggag cagtccatac aaggggtctg gattttgcat gcgacatcta catctgggca   1560
cctctggcag gaacatgtgg cgtgctcctg ctcagcctgg tcatcaccct gtactgcaag   1620
agaggcagga agaagctgct gtatatcttc aagcagccct tcatgcgccc cgtgcagaca   1680
acccaggagg aggatggctg ctcctgtagg ttcccagaag aggaggaggg aggatgtgag   1740
ctgcgcgtga agttttcccg gtctgccgac gcacctgcat accagcaggg ccagaaccag   1800
ctgtataacg agctgaatct gggccggaga gaggagtacg atgtgctgga caagaggcgc   1860
ggcagagatc cagagatggg cggcaagccc cggagaaaga accctcagga gggcctgtac   1920
aatgagctgc agaaggataa gatggccgag gcctattctg agatcggcat gaagggagag   1980
aggcgccggg gcaagggaca cgacggactg taccagggac tgagcacagc caccaaggat   2040
acctatgacg ccctgcatat gcaggcactg cctccaaggt gatctagagg gcccgtttaa   2100
acccgctgat cagcctcgac tgtgccttct agttgccagc catctgttgt ttgcccctcc   2160
cccgtgcctt ccttgaccct ggaaggtgcc actcccactg tcctttccta ataaaatgag   2220
gaaattgcat cgcattgtct gagtaggtgt cattctattc tggggggtgg ggtggggcag   2280
gacagcaagg gggaggattg ggaagacaat agcaggcatg ctggggatgc ggtgggctct   2340
atgactagtg gcgaattccc gtgtaccagc tgagagactc taaatccagt gacaagtctg   2400
tctgcctatt caccgatttt gattctcaaa caaatgtgtc acaaagtaag gattctgatg   2460
tgtatatcac agacaaaact gtgctagaca tgaggtctat ggacttcaag agcaacagtg   2520
ctgtggcctg gagcaacaaa tctgactttg catgtgcaaa cgccttcaac aacagcatta   2580
ttccagaaga caccttcttc cccagcccag gtaagggcag ctttggtgcc ttcgcaggct   2640
gtttccttgc ttcaggaa                                                 2658


<210>  10

<211>  2646
<212>  DNA
<213>  artificial sequence

<220>
<223>  SEQUENCE IN TRAC ANTI-CD22 B-B7 QR3

<400>  10
aagtagccct gcatttcagg tttccttgag tggcaggcca ggcctggccg tgaacgttca     60
ctgaaatcat ggcctcttgg ccaagattga tagcttgtgc ctgtccctga gtcccagtcc    120
atcacgagca gctggtttct aagatgctat ttcccgtata aagcatgaga ccgtgacttg    180
ccagccccac agagccccgc ccttgtccat cactggcatc tggactccag cctgggttgg    240
ggcaaagagg gaaatgagat catgtcctaa ccctgatcct cttgtcccac agatatccag    300
tccggtgagg gcagaggaag tcttctaaca tgcggtgacg tggaggagaa tccgggcccc    360
ggatccgctc tgcccgtcac cgctctgctg ctgccactgg ccctgctgct gcacgccgcc    420
agacccggcg gaggaggctc ttgcccctac agcaacccca gcctgtgctc tggcggcggc    480
ggcagcggag gcggcggctc ccaggtgcag ctgcagcaga gcggccctgg cctggtggag    540
ccaagccaga cactgtccct gacctgcgcc atctctggcg acagcgtgag ctccaacagc    600
gccgcatgga attggatcag gcagtcccca tctcggggcc tggagtggct gggcagaaca    660
tactataggt ccacctggta caacgactat gccggctccg tgaagtctcg catcacaatc    720
aaccccgata ccagcaagaa tcagttctcc ctgcagctga catctgtgac ccctgaggac    780
acagccgtgt actattgcac cagaagcagg cacaatacat ttcggggaat ggacgtgtgg    840
ggacagggca cactggtgac cgtgagcgga ggaggaggat ccggcggagg aggctctggc    900
ggcggcggca gcgacatcca gctgacccag tccccttcta gcctgagcgc ctccgtgggc    960
gatagagtga caatcacctg tagggcctct cagagcatct cctcttacct gaactggtat   1020
cagcagaagc ccggcaaggc ccctaagctg ctgatctacg cagcaagctc cctgcagtct   1080
ggagtgccaa gcagattctc cggctctggc agcggcaccg actttacact gaccatctct   1140
agcctgcagc ctgaggattt cgccacatac tattgccagc agtcctattc tacaccactg   1200
acctttggcg gcggcaccaa ggtggagatc aagggaagcg gcggcggcgg aagttgtcca   1260
tattcaaacc caagtctgtg cagcggcgga ggaggaagcg aactgcctac tcagggaacc   1320
ttcagcaacg tgtccaccaa tgtgagccca gcaaagccta ccacaaccgc atgcccatac   1380
tctaacccca gcctgtgcac aaccacacca gcacccaggc cccctacccc tgcaccaaca   1440
atcgcctccc agcctctgtc tctgcggcca gaggcctgca gacccgccgc cggcggagca   1500
gtgcacacac ggggcctgga ctttgcctgt gatatctata tctgggcacc actggccgga   1560
acatgtggcg tgctgctgct gtcactggtc attacactgt actgtaagcg aggccggaag   1620
aaactgctgt atattttcaa acagcccttt atgagacctg tgcagactac ccaggaggaa   1680
gacggctgca gctgtaggtt ccccgaggaa gaggaaggcg ggtgtgagct gagggtcaag   1740
tttagccgct ccgcagatgc ccctgcttac cagcaggggc agaatcagct gtataacgag   1800
ctgaatctgg gacggagaga ggaatacgac gtgctggata aaaggcgcgg gagagacccc   1860
gaaatgggag gcaagccacg acggaaaaac ccccaggagg gcctgtacaa tgaactgcag   1920
aaggacaaaa tggcagaggc ctatagtgaa atcgggatga agggagagag aaggcgcggc   1980
aaagggcacg atggcctgta ccaggggctg tctactgcca ccaaggacac ctatgatgct   2040
ctgcatatgc aggcactgcc tccaaggtga tctagagggc ccgtttaaac ccgctgatca   2100
gcctcgactg tgccttctag ttgccagcca tctgttgttt gcccctcccc cgtgccttcc   2160
ttgaccctgg aaggtgccac tcccactgtc ctttcctaat aaaatgagga aattgcatcg   2220
cattgtctga gtaggtgtca ttctattctg gggggtgggg tggggcagga cagcaagggg   2280
gaggattggg aagacaatag caggcatgct ggggatgcgg tgggctctat gactagtggc   2340
gaattcccgt gtaccagctg agagactcta aatccagtga caagtctgtc tgcctattca   2400
ccgattttga ttctcaaaca aatgtgtcac aaagtaagga ttctgatgtg tatatcacag   2460
acaaaactgt gctagacatg aggtctatgg acttcaagag caacagtgct gtggcctgga   2520
gcaacaaatc tgactttgca tgtgcaaacg ccttcaacaa cagcattatt ccagaagaca   2580
ccttcttccc cagcccaggt aagggcagct ttggtgcctt cgcaggctgt ttccttgctt   2640
caggaa                                                              2646


<210>  11

<211>  2649
<212>  DNA
<213>  artificial sequence

<220>
<223>  SEQUENCE IN TRAC ANTI-CD22 A-D4 QR3

<400>  11
aagtagccct gcatttcagg tttccttgag tggcaggcca ggcctggccg tgaacgttca     60
ctgaaatcat ggcctcttgg ccaagattga tagcttgtgc ctgtccctga gtcccagtcc    120
atcacgagca gctggtttct aagatgctat ttcccgtata aagcatgaga ccgtgacttg    180
ccagccccac agagccccgc ccttgtccat cactggcatc tggactccag cctgggttgg    240
ggcaaagagg gaaatgagat catgtcctaa ccctgatcct cttgtcccac agatatccag    300
tccggtgagg gcagaggaag tcttctaaca tgcggtgacg tggaggagaa tccgggcccc    360
ggatccgctc tgcccgtcac cgctctgctg ctgccactgg ccctgctgct gcacgccgcc    420
agacccggcg gaggaggctc ttgcccctac agcaacccca gcctgtgctc tggcggcggc    480
ggcagcggag gcggcggctc ccaggtgcag ctgcagcaga gcggccccgg cctggtgaag    540
cctagccaga cactgtccct gacctgcgca atctccggcg acagcgtgtc cggaaacagg    600
gccacatgga attggatcag acagtctcca agcaggggcc tggagtggct gggaaggacc    660
tactatcggt ccgcctggta caacgactat gccgtgtctg tgaagggccg catcacattc    720
aacccagata ccagcaagaa tcagttttcc ctgcagctga attctgtgac acccgaggat    780
accgccgtgt actattgcgc cagaggcgag agcggagcag cagcagacgc cttcgatatc    840
tggggccagg gcaccacagt gacagtgagc ggaggaggag gatccggcgg aggaggctct    900
ggcggcggcg gcagcgacat ccagctgacc cagagcccac cttccctgtc tgccagcgtg    960
ggcgatcgcg tgacaatcac ctgtcgggcc tcccagtcta tcagctccta cctgaactgg   1020
tatcagcaga agccaggcaa ggcccccaag ctgctgatct acgcagcatc tagcctgcag   1080
tctggagtgc caagcagatt cagcggatcc ggattcggca cagactttac actgaccatc   1140
tcctctctgc agcccgagga tttcgccacc tactattgcc agcagtctta tagcacacct   1200
cagacctttg gccagggcac caaggtggac atcaagggaa gtggaggagg aggaagttgt   1260
ccctactcaa acccatctct gtgctcagga ggaggaggaa gtgaactgcc tactcaggga   1320
acattcagca acgtgtccac caatgtgagc ccagcaaagc ctaccacaac cgcatgccca   1380
tactctaacc ccagcctgtg cacaaccaca ccagcaccca ggccccctac ccctgcacca   1440
acaatcgcct cccagcctct gtctctgcgg ccagaggcct gcagacccgc cgccggcgga   1500
gcagtgcaca cacggggcct ggactttgcc tgtgatatct atatctgggc accactggcc   1560
ggaacatgtg gcgtgctgct gctgtcactg gtcattacac tgtactgtaa gcgaggccgg   1620
aagaaactgc tgtatatttt caaacagccc tttatgagac ctgtgcagac tacccaggag   1680
gaagacggct gcagctgtag gttccccgag gaagaggaag gcgggtgtga gctgagggtc   1740
aagtttagcc gctccgcaga tgcccctgct taccagcagg ggcagaatca gctgtataac   1800
gagctgaatc tgggacggag agaggaatac gacgtgctgg ataaaaggcg cgggagagac   1860
cccgaaatgg gaggcaagcc acgacggaaa aacccccagg agggcctgta caatgaactg   1920
cagaaggaca aaatggcaga ggcctatagt gaaatcggga tgaagggaga gagaaggcgc   1980
ggcaaagggc acgatggcct gtaccagggg ctgtctactg ccaccaagga cacctatgat   2040
gctctgcata tgcaggcact gcctccaagg tgatctagag ggcccgttta aacccgctga   2100
tcagcctcga ctgtgccttc tagttgccag ccatctgttg tttgcccctc ccccgtgcct   2160
tccttgaccc tggaaggtgc cactcccact gtcctttcct aataaaatga ggaaattgca   2220
tcgcattgtc tgagtaggtg tcattctatt ctggggggtg gggtggggca ggacagcaag   2280
ggggaggatt gggaagacaa tagcaggcat gctggggatg cggtgggctc tatgactagt   2340
ggcgaattcc cgtgtaccag ctgagagact ctaaatccag tgacaagtct gtctgcctat   2400
tcaccgattt tgattctcaa acaaatgtgt cacaaagtaa ggattctgat gtgtatatca   2460
cagacaaaac tgtgctagac atgaggtcta tggacttcaa gagcaacagt gctgtggcct   2520
ggagcaacaa atctgacttt gcatgtgcaa acgccttcaa caacagcatt attccagaag   2580
acaccttctt ccccagccca ggtaagggca gctttggtgc cttcgcaggc tgtttccttg   2640
cttcaggaa                                                           2649


<210>  12

<211>  2679
<212>  DNA
<213>  artificial

<220>
<223>  SEQUENCE IN TRAC ANTI-CD123 K43 QR3

<400>  12
aagtagccct gcatttcagg tttccttgag tggcaggcca ggcctggccg tgaacgttca     60
ctgaaatcat ggcctcttgg ccaagattga tagcttgtgc ctgtccctga gtcccagtcc    120
atcacgagca gctggtttct aagatgctat ttcccgtata aagcatgaga ccgtgacttg    180
ccagccccac agagccccgc ccttgtccat cactggcatc tggactccag cctgggttgg    240
ggcaaagagg gaaatgagat catgtcctaa ccctgatcct cttgtcccac agatatccag    300
tccggtgagg gcagaggaag tcttctaaca tgcggtgacg tggaggagaa tccgggcccc    360
ggatccgctc tgcccgtcac cgctctgctg ctgcctctgg ccctgctgct gcacgcagcc    420
agaccaggcg gaggaggctc ctgcccttac tctaacccaa gcctgtgctc cggaggagga    480
ggatccggcg gaggaggctc tgaggtgaag ctggtggaga gcggaggagg cctggtgcag    540
cctggcggct ccctgtctct gagctgcgca gcatccggct tcacctttac agactactat    600
atgtcttggg tgagacagcc ccctggcaag gccctggagt ggctggccct gatcaggtcc    660
aaggccgatg gctacaccac agagtattcc gcctctgtga agggcagatt caccctgtct    720
agggacgata gccagtccat cctgtacctg cagatgaatg cactgcgccc cgaggacagc    780
gccacatact attgtgccag agacgccgcc tactattctt actatagccc tgagggcgct    840
atggactact ggggccaggg cacctccgtg acagtgagct ccggaggagg aggaagcgga    900
ggaggaggct ccggcggcgg cggctctatg gccgactata aggatatcgt gatgacccag    960
agccacaagt ttatgtctac aagcgtgggc gaccgcgtga acatcacctg caaggccagc   1020
cagaatgtgg attccgccgt ggcctggtac cagcagaagc ctggccagag ccctaaggcc   1080
ctgatctatt ccgcctctta ccggtatagc ggagtgcctg accgcttcac cggaagggga   1140
tccggaacag acttcaccct gacaatctct agcgtgcagg ccgaggatct ggccgtgtac   1200
tattgtcagc agtactatag caccccctgg accttcggcg gaggaaccaa gctggagatc   1260
aagagaggat ctggaggagg aggaagctgc ccatactcca acccctctct gtgcagcgga   1320
ggaggaggat ctgagctgcc aacccagggc acattttcca acgtgtctac aaatgtgagc   1380
ccagcaaagc caaccacaac cgcatgccct tatagcaatc catccctgtg cacaaccaca   1440
cctgcaccaa gaccaccaac cccagcacct acaatcgcct ctcagccact gagcctgcgc   1500
cccgaggcat gccggcctgc agcaggcggc gccgtgcaca ccaggggcct ggacttcgcc   1560
tgcgatatct acatctgggc acctctggca ggaacctgtg gcgtgctgct gctgagcctg   1620
gtcatcaccc tgtactgcaa gagaggcagg aagaagctgc tgtatatctt caagcagccc   1680
tttatgcgcc ctgtgcagac cacacaggag gaggacggct gcagctgtcg gttcccagaa   1740
gaggaggagg gcggctgtga gctgagagtg aagtttagca ggtccgccga tgcaccagca   1800
taccagcagg gacagaacca gctgtataac gagctgaatc tgggccggag agaggagtac   1860
gacgtgctgg ataagaggag gggaagggac cccgagatgg gaggcaagcc acggagaaag   1920
aacccccagg agggcctgta caatgagctg cagaaggaca agatggccga ggcctattcc   1980
gagatcggca tgaagggaga gaggcgccgg ggcaagggac acgatggcct gtaccagggc   2040
ctgtctaccg ccacaaagga cacctatgat gccctgcata tgcaggcact gcctccaagg   2100
tgatctagag ggcccgttta aacccgctga tcagcctcga ctgtgccttc tagttgccag   2160
ccatctgttg tttgcccctc ccccgtgcct tccttgaccc tggaaggtgc cactcccact   2220
gtcctttcct aataaaatga ggaaattgca tcgcattgtc tgagtaggtg tcattctatt   2280
ctggggggtg gggtggggca ggacagcaag ggggaggatt gggaagacaa tagcaggcat   2340
gctggggatg cggtgggctc tatgactagt ggcgaattcc cgtgtaccag ctgagagact   2400
ctaaatccag tgacaagtct gtctgcctat tcaccgattt tgattctcaa acaaatgtgt   2460
cacaaagtaa ggattctgat gtgtatatca cagacaaaac tgtgctagac atgaggtcta   2520
tggacttcaa gagcaacagt gctgtggcct ggagcaacaa atctgacttt gcatgtgcaa   2580
acgccttcaa caacagcatt attccagaag acaccttctt ccccagccca ggtaagggca   2640
gctttggtgc cttcgcaggc tgtttccttg cttcaggaa                          2679


<210>  13

<211>  22
<212>  DNA
<213>  artificial sequence

<220>
<223>  plus strand first PCR

<400>  13
ataccgttat taacatatga ca                                              22


<210>  14

<211>  21
<212>  DNA
<213>  artificial sequence

<220>
<223>  plus strand second PCR

<400>  14
catatgacaa ctcaattaaa c                                               21


<210>  15

<211>  34
<212>  DNA
<213>  artificial sequence

<220>
<223>  oligo rev compl - plus

<400>  15
ataccgttat taacatatga caactcaatt aaac                                 34


<210>  16

<211>  22
<212>  DNA
<213>  artificial sequence

<220>
<223>  1st PCR plus

<400>  16
ataccgttat taacatatga ca                                              22


<210>  17

<211>  21
<212>  DNA
<213>  artificial sequence

<220>
<223>  2nd PCR plus

<400>  17
catatgacaa ctcaattaaa c                                               21


<210>  18

<211>  29
<212>  DNA
<213>  artificial sequence

<220>
<223>  minus strand first PCR

<400>  18
gtttaattga gttgtcatat gttaataac                                       29


<210>  19

<211>  27
<212>  DNA
<213>  artificial sequence

<220>
<223>  minus strand second PCR

<400>  19
ttgagttgtc atatgttaat aacggta                                         27


<210>  20

<211>  34
<212>  DNA
<213>  artificial sequence

<220>
<223>  oligo rev compl - minus

<400>  20
gtttaattga gttgtcatat gttaataacg gtat                                 34


<210>  21

<211>  29
<212>  DNA
<213>  artificial sequence

<220>
<223>  1st PCR minus

<400>  21
gtttaattga gttgtcatat gttaataac                                       29


<210>  22

<211>  27
<212>  DNA
<213>  artificial sequence

<220>
<223>  2nd PCR minus

<400>  22
ttgagttgtc atatgttaat aacggta                                         27


<210>  23

<211>  588
<212>  DNA
<213>  artificial sequence

<220>
<223>  IRES EMCV

<400>  23
gcccctctcc ctcccccccc cctaacgtta ctggccgaag ccgcttggaa taaggccggt     60
gtgcgtttgt ctatatgtta ttttccacca tattgccgtc ttttggcaat gtgagggccc    120
ggaaacctgg ccctgtcttc ttgacgagca ttcctagggg tctttcccct ctcgccaaag    180
gaatgcaagg tctgttgaat gtcgtgaagg aagcagttcc tctggaagct tcttgaagac    240
aaacaacgtc tgtagcgacc ctttgcaggc agcggaaccc cccacctggc gacaggtgcc    300
tctgcggcca aaagccacgt gtataagata cacctgcaaa ggcggcacaa ccccagtgcc    360
acgttgtgag ttggatagtt gtggaaagag tcaaatggct ctcctcaagc gtattcaaca    420
aggggctgaa ggatgcccag aaggtacccc attgtatggg atctgatctg gggcctcggt    480
gcacatgctt tacatgtgtt tagtcgaggt taaaaaaacg tctaggcccc ccgaaccacg    540
gggacgtggt tttcctttga aaaacacgat gataatatgg ccacaacc                 588



