                         SEQUENCE LISTING

<110>  Katholieke Universiteit Leuven
       Gijsbers, Rik
       De Rijck, Jan
 
<120>  MLV-BASED GENE THERAPY WITH MUTATED INTEGRASE COUPLED TO PEPTIDES

<130>  ZL915132

<150>  GB1617599.4
<151>  2016-10-18

<160>  26    

<170>  PatentIn version 3.5

<210>  1
<211>  54
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  chromatin binding fragment of LANA

<400>  1

Glu Tyr Val Val Glu Lys Val Leu Asp Arg Arg Val Val Lys Gly Lys 
1               5                   10                  15      


Val Glu Tyr Leu Leu Lys Trp Lys Gly Phe Ser Asp Glu Asp Asn Thr 
            20                  25                  30          


Trp Glu Pro Glu Glu Asn Leu Asp Cys Pro Asp Leu Ile Ala Glu Phe 
        35                  40                  45              


Leu Gln Ser Gln Lys Thr 
    50                  


<210>  2
<211>  60
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  chromatin binding fragment of CBX1

<400>  2

Met Thr Phe Gln Ala Ser His Arg Ser Ala Trp Gly Lys Ser Arg Lys 
1               5                   10                  15      


Lys Asn Trp Gln Tyr Glu Gly Pro Thr Gln Lys Leu Phe Leu Lys Arg 
            20                  25                  30          


Asn Asn Val Ser Ala Pro Asp Gly Pro Ser Asp Pro Ser Ile Ser Val 
        35                  40                  45              


Ser Ser Glu Gln Ser Gly Ala Gln Gln Pro Pro Ala 
    50                  55                  60  


<210>  3
<211>  16
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  chromatin binding fragment of CDYL

<400>  3

Gln Thr Glu Thr Lys Gly Arg Arg Tyr Gly Arg Arg Pro Ser Ser Arg 
1               5                   10                  15      


<210>  4
<211>  31
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  chromatin binding fragment of HPV8 E2

<400>  4

Met Ala Pro Pro Gly Met Arg Leu Arg Ser Gly Arg Ser Thr Gly Ala 
1               5                   10                  15      


Pro Leu Thr Arg Gly Ser Cys Arg Lys Arg Asn Arg Ser Pro Glu 
            20                  25                  30      


<210>  5
<211>  28
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  MLV fragment

<400>  5

Gly Gly Gly Pro Ser Ser Arg Leu Thr Trp Arg Val Gln Arg Ser Gln 
1               5                   10                  15      


Asn Pro Leu Lys Ile Arg Leu Thr Arg Glu Ala Pro 
            20                  25              


<210>  6
<211>  24
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  AKR-MLV fragment

<400>  6

Pro Ile Lys Pro Ser Trp Arg Val Gln Arg Ser Gln Asn Pro Leu Lys 
1               5                   10                  15      


Ile Arg Leu Thr Arg Gly Ala Pro 
            20                  


<210>  7
<211>  26
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  BaEV fragment

<400>  7

Thr Pro Gly Pro Thr Ser Ser Gly Thr Trp Arg Leu Arg Arg Ser Glu 
1               5                   10                  15      


Asp Pro Leu Lys Ile Arg Leu Ser Arg Thr 
            20                  25      


<210>  8
<211>  25
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  CAS-BR-E fragment

<400>  8

Ser Pro Ala Arg Thr Ala Trp Lys Val Gln Arg Ser Gln Asn Pro Leu 
1               5                   10                  15      


Lys Ile Arg Leu Ser Arg Glu Pro Ser 
            20                  25  


<210>  9
<211>  26
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  en-FeLV  fragment

<400>  9

Thr Pro Gly Pro Glu Thr Pro Lys Thr Trp Lys Leu His Arg Ser Glu 
1               5                   10                  15      


Asn Pro Leu Lys Ile Arg Leu Ser Arg Val 
            20                  25      


<210>  10
<211>  29
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  GaLV fragment

<400>  10

Ser Ala Pro Asp Glu Ser Trp Glu Leu Glu Lys Thr Asp His Pro Leu 
1               5                   10                  15      


Lys Leu Arg Ile Arg Arg Arg Arg Asp Glu Ser Ala Lys 
            20                  25                  


<210>  11
<211>  29
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  KoRV fragment

<400>  11

Gly Ala Pro Asp Glu Ser Trp Glu Leu Glu Lys Thr Asp His Pro Leu 
1               5                   10                  15      


Lys Leu Arg Val Arg Arg Arg Arg Asn Glu Ser Thr Ala 
            20                  25                  


<210>  12
<211>  33
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  PERV fragment


<220>
<221>  misc_feature
<222>  (33)..(33)
<223>  Xaa can be any naturally occurring amino acid

<400>  12

Pro Asp Ser Gly Trp Arg Ala Glu Lys Thr Glu Asn Pro Leu Lys Leu 
1               5                   10                  15      


Arg Leu His Arg Leu Val Pro Tyr Ser Asn Asn Asn Ser Pro Gly Gln 
            20                  25                  30          


Xaa 
    


<210>  13
<211>  37
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  chromodomain consensus sequence

<400>  13

Tyr Val Val Glu Lys Val Leu Asp Arg Arg Val Val Lys Gly Lys Val 
1               5                   10                  15      


Glu Tyr Leu Leu Lys Trp Lys Gly Phe Ser Asp Glu Asp Asn Thr Trp 
            20                  25                  30          


Lys Pro Lys Glu Asn 
        35          


<210>  14
<211>  189
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  CBX1 fragment

<400>  14
cataacgatc gagagtacgt ggtggagaag gtgctggacc gccgcgtggt gaagggcaag       60

gtggagtacc tgctgaagtg gaagggcttc agcgacgagg acaacacctg ggagcccgag      120

gagaacctgg actgccccga cctgatcgcc gagttcctgc agagccagaa gacctagcgg      180

ccgccataa                                                              189


<210>  15
<211>  207
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  CDYL fragment

<400>  15
cataacgatc gaatgacctt ccaggccagc caccgcagcg cctggggcaa gagccgcaag       60

aagaactggc agtacgaggg ccccacccag aagctgttcc tgaagcgcaa caacgtgagc      120

gcccccgacg gccccagcga ccccagcatc agcgtgagca gcgagcagag cggcgcccag      180

cagccccccg cctagcggcc gccataa                                          207


<210>  16
<211>  50
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  E2 fwd primer

<400>  16
cagaccgaga ccaagggccg ccgctacggc cgccgcccca gcagccgcta                  50


<210>  17
<211>  53
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  E2 reverse primer

<400>  17
ctagcggctg ctggggcggc ggccgtagcg gcggcccttg gtctcggtct gat              53


<210>  18
<211>  95
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  LANA fw primer

<400>  18
atggcccctc ctggcatgcg cctgcgcagt ggccgcagca ccggcgcgcc tctgacccgc       60

ggcagctgcc gcaagcgcaa ccgcagcccc gagta                                  95


<210>  19
<211>  101
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  LANA reverse primer

<400>  19
ggcctactcg gggctgcggt tgcgcttgcg gcagctgccg cgggtcagag gcgcgccggt       60

gctgcggcca ctgcgcaggc gcatgccagg agggccatat g                          101


<210>  20
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  eGFP forward primer

<400>  20
ggagcgcacc atcttcttca                                                   20


<210>  21
<211>  18
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  eGFP reverse primer

<400>  21
aggggtgtcg ccctcgaa                                                     18


<210>  22
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  eGFP probe

<400>  22
gctacaagac ccgcgccgag g                                                 21


<210>  23
<211>  28
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Integrase WT C terminal fragment

<400>  23

Gly Gly Gly Pro Ser Ser Arg Leu Thr Trp Arg Val Gln Arg Ser Gln 
1               5                   10                  15      


Asn Pro Leu Lys Ile Arg Leu Thr Arg Glu Ala Pro 
            20                  25              


<210>  24
<211>  28
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  integrase C terminal fragment W390A mutation

<400>  24

Gly Gly Gly Pro Ser Ser Arg Leu Thr Ala Arg Val Gln Arg Ser Gln 
1               5                   10                  15      


Asn Pro Leu Lys Ile Arg Leu Thr Arg Glu Ala Pro 
            20                  25              


<210>  25
<211>  82
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  integrase C terminal fragment W390A mutation  + CBX1 fragment

<400>  25

Gly Gly Gly Pro Ser Ser Arg Leu Thr Ala Arg Val Gln Arg Ser Gln 
1               5                   10                  15      


Asn Pro Leu Lys Ile Arg Leu Thr Arg Glu Ala Pro Glu Tyr Val Val 
            20                  25                  30          


Glu Lys Val Leu Asp Arg Arg Val Val Lys Gly Lys Val Glu Tyr Leu 
        35                  40                  45              


Leu Lys Trp Lys Gly Phe Ser Asp Glu Asp Asn Thr Trp Glu Pro Glu 
    50                  55                  60                  


Glu Asn Leu Asp Cys Pro Asp Leu Ile Ala Glu Phe Leu Gln Ser Gln 
65                  70                  75                  80  


Lys Thr 
        


<210>  26
<211>  58
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  integrase C terminal fragment W390A mutation + LANA fragment

<400>  26

Gly Gly Gly Pro Ser Ser Arg Leu Thr Ala Arg Val Gln Arg Ser Gln 
1               5                   10                  15      


Asn Pro Leu Lys Ile Arg Leu Thr Arg Glu Ala Pro Met Ala Pro Pro 
            20                  25                  30          


Gly Met Arg Leu Arg Ser Gly Arg Ser Thr Gly Ala Pro Leu Thr Arg 
        35                  40                  45              


Gly Ser Cys Arg Lys Arg Asn Arg Ser Pro 
    50                  55              


