                         SEQUENCE LISTING

<110>  VIB VZW
       UNIVERSITEIT GENT
 
<120>  Means and methods to treat inflammation

<130>  CL/MMP8T/562

<150>  GB 1617622.4
<151>  2016-10-18

<160>  28    

<170>  PatentIn version 3.5

<210>  1
<211>  10
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  MMP8 inactivating antigen binding polypeptide - CDR1

<400>  1

Gly Phe Thr Leu Asp Tyr Tyr Asn Ile Gly 
1               5                   10  


<210>  2
<211>  17
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  MMP8 inactivating antigen binding polypeptide - CDR2

<400>  2

Cys Ile Ser Ser Ser Gly Gly Arg Thr Asn Tyr Ala Asp Ser Val Lys 
1               5                   10                  15      


Gly 
    


<210>  3
<211>  12
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  MMP8 inactivating antigen binding polypeptide - CDR3

<400>  3

Cys Met Ala Thr Thr Glu Gly Tyr Glu Tyr Asp Tyr 
1               5                   10          


<210>  4
<211>  10
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  TNFR1 inactivating antigen binding polypeptide - CDR1

<400>  4

Val Arg Thr Phe Ser Ser Tyr Ala Met Gly 
1               5                   10  


<210>  5
<211>  17
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  TNFR1 inactivating antigen binding polypeptide - CDR2

<400>  5

Ala Ile Ser Trp Ser Gly Gly Ser Thr Tyr Tyr Ala Asp Ser Val Lys 
1               5                   10                  15      


Gly 
    


<210>  6
<211>  10
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  TNFR1 inactivating antigen binding polypeptide - CDR3

<400>  6

Leu His Glu Asp Leu Tyr Glu Tyr Asp Tyr 
1               5                   10  


<210>  7
<211>  384
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  a tri-specific nanobody construct with the structure anti-TNFR1- 
       anti-Albumine- anti-MMP8

<400>  7

Gln Val Gln Leu Gln Glu Ser Gly Gly Gly Leu Val Gln Ala Gly Gly 
1               5                   10                  15      


Pro Leu Arg Leu Ser Cys Ala Ala Ser Val Arg Thr Phe Ser Ser Tyr 
            20                  25                  30          


Ala Met Gly Trp Phe Arg Gln Ala Pro Gly Lys Glu Arg Glu Phe Val 
        35                  40                  45              


Ala Ala Ile Ser Trp Ser Gly Gly Ser Thr Tyr Tyr Ala Asp Ser Val 
    50                  55                  60                  


Lys Gly Arg Phe Thr Ile Ser Arg Asp Asn Ala Lys Asn Thr Val Tyr 
65                  70                  75                  80  


Leu Gln Met Asn Ser Leu Lys Pro Glu Asp Thr Ala Val Tyr Tyr Cys 
                85                  90                  95      


Ala Val Leu His Glu Asp Leu Tyr Glu Tyr Asp Tyr Trp Gly Gln Gly 
            100                 105                 110         


Thr Gln Val Thr Val Ser Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly 
        115                 120                 125             


Ser Gly Gly Gly Gly Ser Gln Val Gln Leu Gln Glu Ser Gly Gly Gly 
    130                 135                 140                 


Leu Val Gln Pro Gly Gly Ser Leu Arg Leu Ser Cys Glu Ala Ser Gly 
145                 150                 155                 160 


Phe Thr Phe Ser Arg Phe Gly Met Thr Trp Val Arg Gln Ala Pro Gly 
                165                 170                 175     


Lys Gly Val Glu Trp Val Ser Gly Ile Ser Ser Leu Gly Asp Ser Thr 
            180                 185                 190         


Leu Tyr Ala Asp Ser Val Lys Gly Arg Phe Thr Ile Ser Arg Asp Asn 
        195                 200                 205             


Ala Lys Asn Thr Leu Tyr Leu Gln Met Asn Ser Leu Lys Pro Glu Asp 
    210                 215                 220                 


Thr Ala Val Tyr Tyr Cys Thr Ile Gly Gly Ser Leu Asn Pro Gly Gly 
225                 230                 235                 240 


Gln Gly Thr Gln Val Thr Val Ser Ser Gly Gly Gly Gly Ser Gly Gly 
                245                 250                 255     


Gly Gly Ser Gly Gly Gly Gly Ser Gln Val Gln Leu Gln Glu Ser Gly 
            260                 265                 270         


Gly Gly Leu Val Gln Pro Gly Gly Ser Leu Arg Leu Ser Cys Thr Ala 
        275                 280                 285             


Ser Gly Phe Thr Leu Asp Tyr Tyr Asn Ile Gly Trp Phe Arg Gln Ala 
    290                 295                 300                 


Pro Gly Lys Glu Arg Glu Arg Val Ser Cys Ile Ser Ser Ser Gly Gly 
305                 310                 315                 320 


Arg Thr Asn Tyr Ala Asp Ser Val Lys Gly Arg Phe Thr Ile Ser Arg 
                325                 330                 335     


Asp Asn Ala Lys Asn Thr Val Tyr Leu Gln Met Asn Ser Leu Lys Pro 
            340                 345                 350         


Glu Asp Thr Gly Val Tyr Tyr Cys Ala His Cys Met Ala Thr Thr Glu 
        355                 360                 365             


Gly Tyr Glu Tyr Asp Tyr Trp Gly Gln Gly Thr Gln Val Thr Val Ser 
    370                 375                 380                 


<210>  8
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer

<400>  8
attcccaagg agtgtccaag c                                                 21


<210>  9
<211>  24
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer

<400>  9
tgattgtcat atctccagca ctgg                                              24


<210>  10
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer

<400>  10
ccgggagaag agggatagct t                                                 21


<210>  11
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer

<400>  11
tcggacagtc actcaccaag t                                                 21


<210>  12
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer

<400>  12
ttttgatgcc gaagaaacat gga                                               23


<210>  13
<211>  19
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer

<400>  13
gtgagcgagc cccaaagaa                                                    19


<210>  14
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer

<400>  14
cctcaggggt tattggactg g                                                 21


<210>  15
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer

<400>  15
ggggacacac actatctctc t                                                 21


<210>  16
<211>  19
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer

<400>  16
tgaagcaggc atctgaggg                                                    19


<210>  17
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer

<400>  17
cgaaggtgga agagtgggag                                                   20


<210>  18
<211>  24
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer

<400>  18
aggtcaaaca ggaagacaga cgta                                              24


<210>  19
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer

<400>  19
tcacacccaa gaacaagcac a                                                 21


<210>  20
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer

<400>  20
agtgttggat acaggccaga c                                                 21


<210>  21
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer

<400>  21
cgtgattcaa atccctgaag t                                                 21


<210>  22
<211>  18
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer

<400>  22
cctgctgctc tcaaggtt                                                     18


<210>  23
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer

<400>  23
tggttgtcac tgcctcgtac tt                                                22


<210>  24
<211>  25
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer

<400>  24
catgctcaac atctccccct tctcc                                             25


<210>  25
<211>  25
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer

<400>  25
gggaaggtgt aatccgtctc cacag                                             25


<210>  26
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer

<400>  26
cctggaggag aagaggaaag aga                                               23


<210>  27
<211>  25
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer

<400>  27
ttgaggacct ctgtgtattt gtcaa                                             25


<210>  28
<211>  1152
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  a tri-specific nanobody construct with the structure anti-TNFR1- 
       anti-Albumine- anti-MMP8

<400>  28
caggtgcagc tgcaggagtc tgggggagga ttggtgcagg ctgggggccc tctgagactc       60

tcctgtgcag cctctgtacg caccttcagt agctatgcca tgggctggtt ccgccaggct      120

ccagggaagg agcgtgagtt tgtagcagct attagctgga gtggtggtag cacatactat      180

gcagactccg tgaagggccg attcaccatc tccagagaca acgccaagaa cacggtgtat      240

ctgcaaatga acagcctgaa acctgaggac acggccgttt attactgtgc agtcctgcac      300

gaggatctat atgagtatga ctactggggc caggggaccc aggtcaccgt ctcctcaggc      360

ggaggcggta gtggcggagg tggatctgga ggcggcggta gtcaggtgca gctacaggag      420

tctgggggag gcttggtgca gcctgggggg tctctgagac tctcctgtga agcctctgga      480

ttcaccttca gcagatttgg catgacctgg gtccgccagg ctccaggaaa gggcgtggag      540

tgggtctcgg gtattagcag tctgggtgat agcacactct atgcggactc cgtgaagggc      600

cgattcacca tctccagaga caacgccaag aacacactgt atctgcaaat gaacagcctg      660

aaacctgagg acacggccgt gtattactgt acaatagggg gatcgctcaa ccctggcggc      720

caggggaccc aggtcacagt ctcctcaggc ggaggcggta gtggcggagg tggatctgga      780

ggcggcggta gtcaggtgca gctgcaggag tctgggggag gcttggtgca gcctgggggg      840

tctctgagac tctcctgtac agcctctgga ttcactttgg attattataa cataggctgg      900

ttccgccagg ccccagggaa ggagcgtgaa cgggtctcat gtattagtag tagtggtggt      960

agaacaaact atgcagactc cgtgaagggc cgattcacca tctccagaga caacgccaag     1020

aacacggtgt atctgcaaat gaacagcctg aaacctgagg acacaggcgt ttattactgt     1080

gcgcattgta tggcgactac ggagggctat gagtatgact actggggcca ggggacccag     1140

gtcaccgtct cc                                                         1152


