               SEQUENCE LISTING

<110> DSM IP Assets B.V.

<120> EUKARYOTIC CELL COMPRISING XYLOSE ISOMERASE

<130> 32256-WO-PCT

<150> EP16194660.3
<151> 2016-10-19

<160> 171

<170> BiSSAP 1.3.6

<210> 1
<211> 86
<212> DNA
<213> Saccharomyces cerevisiae


<220> 
<223> 5924 PGI1 promoter fragment

<400> 1
actatatgtg aaggcatggc tatggcacgg cagacattcc gccagatcat caataggcac     60

cagggcacta cttctacaca tcaacg                                          86


<210> 2
<211> 29
<212> DNA
<213> Saccharomyces cerevisiae


<220> 
<223> 5925 PGI1 promoter fragment

<400> 2
ttttaggctg gtatcttgat tctaaatcg                                      29


<210> 3
<211> 55
<212> DNA
<213> Saccharomyces cerevisiae


<220> 
<223> 5926 TAL2 ORF fragment

<400> 3
tcgatttaga atcaagatac cagcctaaaa atgtcagaac cttcagagaa aaaac          55


<210> 4
<211> 89
<212> DNA
<213> Saccharomyces cerevisiae


<220> 
<223> 5927 TAL2 ORF fragment

<400> 4
gttgaacatt cttaggctgg tcgaatcatt tagacacggg catcgtcctc tcgaaaggtg     60

gcccaagagg atattaagta ctaatgtgg                                       89


<210> 5
<211> 25
<212> DNA
<213> Artificial Sequence


<220> 
<223> 3847 fusion-PCR of pPGI1 and TAL2

<400> 5
actatatgtg aaggcatggc tatgg                                          25


<210> 6
<211> 27
<212> DNA
<213> Artificial Sequence


<220> 
<223> 3276 fusion-PCR of pPGI1 and NQM1

<400> 6
gttgaacatt cttaggctgg tcgaatc                                        27


<210> 7
<211> 81
<212> DNA
<213> Saccharomyces cerevisiae


<220> 
<223> 5928 TPI1 promoter fragment

<400> 7
cacctttcga gaggacgatg cccgtgtcta aatgattcga ccagcctaag aatgttcaac     60

gcggccgtgt ttaaagatta c                                               81


<210> 8
<211> 18
<212> DNA
<213> Saccharomyces cerevisiae


<220> 
<223> 5929 TPI1 promoter fragment

<400> 8
ccgcggagtt tatgtatg                                                  18


<210> 9
<211> 60
<212> DNA
<213> Saccharomyces cerevisiae


<220> 
<223> 5930 RKI1 ORF fragment

<400> 9
cttaaatcta taactacaaa aaacacatac ataaactccg cggatggctg ccggtgtccc     60


<210> 10
<211> 97
<212> DNA
<213> Saccharomyces cerevisiae


<220> 
<223> 5931 RKI1 ORF fragment

<400> 10
ctagcgtgtc ctcgcatagt tcttagattg tcgctacggc atatacgatc cgtgagacgt     60

atcataggtg agaaagagat ggagaatgta gtactgc                              97


<210> 11
<211> 20
<212> DNA
<213> Artificial Sequence


<220> 
<223> 4672 fusion-PCR of pTPI1 and RKI1

<400> 11
cacctttcga gaggacgatg                                                20


<210> 12
<211> 30
<212> DNA
<213> Artificial Sequence


<220> 
<223> 3277 fusion-PCR of pTPI1 and RKI1

<400> 12
ctagcgtgtc ctcgcatagt tcttagattg                                     30


<210> 13
<211> 87
<212> DNA
<213> Saccharomyces cerevisiae


<220> 
<223> 5932 PYK1 promoter fragment

<400> 13
acgtctcacg gatcgtatat gccgtagcga caatctaaga actatgcgag gacacgctag     60

ggtagcgccc tggtcaaact tcagaac                                         87


<210> 14
<211> 33
<212> DNA
<213> Saccharomyces cerevisiae


<220> 
<223> 5933 PYK1 promoter fragment

<400> 14
tgtgatgatg ttttatttgt tttgattggt gtc                                  33


<210> 15
<211> 63
<212> DNA
<213> Saccharomyces cerevisiae


<220> 
<223> 5934 TKL2 ORF fragment

<400> 15
caccaatcaa aacaaataaa acatcatcac aatggcacag ttctccgaca ttgataaact     60

tgc                                                                   63


<210> 16
<211> 80
<212> DNA
<213> Saccharomyces cerevisiae


<220> 
<223> 5935 TKL2 ORF fragment

<400> 16
tgccgaactt tccctgtatg aagcgatctg accaatcctt tgccgtagtt tcaacgtatg     60

gcagcccata cactcaaagc                                                 80


<210> 17
<211> 22
<212> DNA
<213> Artificial Sequence


<220> 
<223> 3283 fusion-PCR of pPYK1 and TKL2

<400> 17
acgtctcacg gatcgtatat gc                                             22


<210> 18
<211> 24
<212> DNA
<213> Artificial Sequence


<220> 
<223> 3288 fusion-PCR of pPYK1 and TKL2

<400> 18
tgccgaactt tccctgtatg aagc                                           24


<210> 19
<211> 87
<212> DNA
<213> Saccharomyces cerevisiae


<220> 
<223> 5912 TDH3 promoter fragment

<400> 19
gccagaggta tagacatagc cagacctacc taattggtgc atcaggtggt catggccctt     60

ccgggagttt atcattatca atactcg                                         87


<210> 20
<211> 21
<212> DNA
<213> Saccharomyces cerevisiae


<220> 
<223> 5913 TDH3 promoter fragment

<400> 20
ccgtcgaaac taagttcttg g                                              21


<210> 21
<211> 66
<212> DNA
<213> Saccharomyces cerevisiae


<220> 
<223> 5914 RPE1 ORF fragment

<400> 21
ttagttttaa aacaccaaga acttagtttc gacggatggt caaaccaatt atagctccca     60

gtatcc                                                                66


<210> 22
<211> 82
<212> DNA
<213> Saccharomyces cerevisiae


<220> 
<223> 5915 RPE1 ORF fragment

<400> 22
gtcacgggtt ctcagcaatt cgagctatta ccgatgatgg ctgaggcgtt agagtaatct     60

cttctccggc ctccatcacc ac                                              82


<210> 23
<211> 21
<212> DNA
<213> Artificial Sequence


<220> 
<223> 4870 fusion-PCR of pTDH3and RPE1

<400> 23
gccagaggta tagacatagc c                                              21


<210> 24
<211> 22
<212> DNA
<213> Artificial Sequence


<220> 
<223> 3290 fusion-PCR of pTDH3and RPE1

<400> 24
gtcacgggtt ctcagcaatt cg                                             22


<210> 25
<211> 84
<212> DNA
<213> Saccharomyces cerevisiae


<220> 
<223> 5916 PGK1 promoter fragment

<400> 25
agattactct aacgcctcag ccatcatcgg taatagctcg aattgctgag aacccgtgac     60

tgcccttatc ttgtgcagtt agac                                            84


<210> 26
<211> 38
<212> DNA
<213> Saccharomyces cerevisiae


<220> 
<223> 5917 PGK1 promoter fragment

<400> 26
tgttttatat ttgttgtaaa aagtagataa ttacttcc                             38


<210> 27
<211> 66
<212> DNA
<213> Saccharomyces cerevisiae


<220> 
<223> 5918 TKL1 ORF fragment

<400> 27
ggaagtaatt atctactttt tacaacaaat ataaaacaat gactcaattc actgacattg     60

ataagc                                                                66


<210> 28
<211> 83
<212> DNA
<213> Saccharomyces cerevisiae


<220> 
<223> 5919 TKL1 ORF fragment

<400> 28
gcctacggtt cccgaagtat gctgctgatg tctggctata cctatccgtc tacgtgaata     60

atgaatgcga ccgatatttt tgg                                             83


<210> 29
<211> 23
<212> DNA
<213> Artificial Sequence


<220> 
<223> 3291 fusion-PCR of pPGK1 and TKL1

<400> 29
ctctaacgcc tcagccatca tcg                                            23


<210> 30
<211> 22
<212> DNA
<213> Artificial Sequence


<220> 
<223> 4068 fusion-PCR of pPGK1 and TKL1

<400> 30
gcctacggtt cccgaagtat gc                                             22


<210> 31
<211> 89
<212> DNA
<213> Artificial Sequence


<220> 
<223> 5920 TEF1 promoter fragment

<400> 31
tattcacgta gacggatagg tatagccaga catcagcagc atacttcggg aaccgtaggc     60

agctcatagc ttcaaaatgt ttctactcc                                       89


<210> 32
<211> 40
<212> DNA
<213> Artificial Sequence


<220> 
<223> 5921 TEF1 promoter fragment

<400> 32
aaaacttaga ttagattgct atgctttctt tctaatgagc                           40


<210> 33
<211> 71
<212> DNA
<213> Saccharomyces cerevisiae


<220> 
<223> 5922 TAL1 ORF fragment

<400> 33
gctcattaga aagaaagcat agcaatctaa tctaagtttt atgtctgaac cagctcaaaa     60

gaaacaaaag g                                                          71


<210> 34
<211> 92
<212> DNA
<213> Saccharomyces cerevisiae


<220> 
<223> 5923 TAL1 ORF fragment

<400> 34
gtgcctattg atgatctggc ggaatgtctg ccgtgccata gccatgcctt cacatatagt     60

cattgtgatc ctcctatgtt gtagtatagt gc                                   92


<210> 35
<211> 26
<212> DNA
<213> Artificial Sequence


<220> 
<223> 3274 fusion-PCR of pTEF1 and TAL1

<400> 35
tattcacgta gacggatagg tatagc                                         26


<210> 36
<211> 26
<212> DNA
<213> Artificial Sequence


<220> 
<223> 3275 fusion-PCR of pTEF1 and TAL1

<400> 36
gtgcctattg atgatctggc ggaatg                                         26


<210> 37
<211> 78
<212> DNA
<213> Saccharomyces cerevisiae


<220> 
<223> 6278 XKS1 ORF fragment

<400> 37
gcaatgacaa attcaaaaga agacgccgac atagaggaga agcatatgta caatgagccg     60

gtccagtgct tccacatc                                                   78


<210> 38
<211> 67
<212> DNA
<213> Saccharomyces cerevisiae


<220> 
<223> 6279 XKS1 ORF fragment

<400> 38
gctcattaga aagaaagcat agcaatctaa tctaagtttt atgttgtgtt cagtaattca     60

gagacag                                                               67


<210> 39
<211> 80
<212> DNA
<213> Artificial Sequence


<220> 
<223> 7133 fl_RPE1_H fragment fw

<400> 39
tataatattt cattatcgga actctagatt ctatacttgt ttcccaattg ttgctggtag     60

ggcccttccg ggagtttatc                                                 80


<210> 40
<211> 22
<212> DNA
<213> Artificial Sequence


<220> 
<223> 3290 fl_RPE1_H fragment rv

<400> 40
gtcacgggtt ctcagcaatt cg                                             22


<210> 41
<211> 23
<212> DNA
<213> Artificial Sequence


<220> 
<223> 3291 H_TKL1_I fragment fw

<400> 41
ctctaacgcc tcagccatca tcg                                            23


<210> 42
<211> 22
<212> DNA
<213> Artificial Sequence


<220> 
<223> 4068 H_TKL1_I fragment rv

<400> 42
gcctacggtt cccgaagtat gc                                             22


<210> 43
<211> 26
<212> DNA
<213> Artificial Sequence


<220> 
<223> 3274 I_TAL1_A fragment

<400> 43
tattcacgta gacggatagg tatagc                                         26


<210> 44
<211> 26
<212> DNA
<213> Artificial Sequence


<220> 
<223> 3275 I_TAL1_A fragment

<400> 44
gtgcctattg atgatctggc ggaatg                                         26


<210> 45
<211> 25
<212> DNA
<213> Artificial Sequence


<220> 
<223> 3847 A_ NQM1 _B

<400> 45
actatatgtg aaggcatggc tatgg                                          25


<210> 46
<211> 27
<212> DNA
<213> Artificial Sequence


<220> 
<223> 3276 A_ NQM1_B

<400> 46
gttgaacatt cttaggctgg tcgaatc                                        27


<210> 47
<211> 20
<212> DNA
<213> Artificial Sequence


<220> 
<223> 4672 B_RKI1_C fragment

<400> 47
cacctttcga gaggacgatg                                                20


<210> 48
<211> 30
<212> DNA
<213> Artificial Sequence


<220> 
<223> 3277 B_RKI1_C fragment

<400> 48
ctagcgtgtc ctcgcatagt tcttagattg                                     30


<210> 49
<211> 22
<212> DNA
<213> Artificial Sequence


<220> 
<223> 3283 C_TKL2_F fragment

<400> 49
acgtctcacg gatcgtatat gc                                             22


<210> 50
<211> 24
<212> DNA
<213> Artificial Sequence


<220> 
<223> 3288 C_TKL2_F fragment

<400> 50
tgccgaactt tccctgtatg aagc                                           24


<210> 51
<211> 79
<212> DNA
<213> Artificial Sequence


<220> 
<223> 7138 F_xylA_P fragment

<400> 51
ctgatagtgc tgtaagtcgc ctccatctta gcagagctgt ccctgaatgc gtactcgtga     60

gcgataccct gcgatcttc                                                  79


<210> 52
<211> 78
<212> DNA
<213> Artificial Sequence


<220> 
<223> 7136 F_xylA_P fragment

<400> 52
catacgttga aactacggca aaggattggt cagatcgctt catacaggga aagttcggca     60

cgcgcagatt agcgaagc                                                   78


<210> 53
<211> 79
<212> DNA
<213> Artificial Sequence


<220> 
<223> 7139 P_xylA_Q fragment

<400> 53
gagctgaatg tatatgctgc gggatcattg cacagctctg agagccctgc aacgcgatat     60

gcgataccct gcgatcttc                                                  79


<210> 54
<211> 78
<212> DNA
<213> Artificial Sequence


<220> 
<223> 7137 P_xylA_Q fragment

<400> 54
tcacgagtac gcattcaggg acagctctgc taagatggag gcgacttaca gcactatcag     60

cgcgcagatt agcgaagc                                                   78


<210> 55
<211> 79
<212> DNA
<213> Artificial Sequence


<220> 
<223> 7142 Q_xylA_E fragment

<400> 55
agcgatctgc gagaccgtat agccatgacg aggtcgcaat cttgcggaca gtgtagctca     60

gcgataccct gcgatcttc                                                  79


<210> 56
<211> 78
<212> DNA
<213> Artificial Sequence


<220> 
<223> 7140 Q_xylA_E fragment

<400> 56
atatcgcgtt gcagggctct cagagctgtg caatgatccc gcagcatata cattcagctc     60

cgcgcagatt agcgaagc                                                   78


<210> 57
<211> 78
<212> DNA
<213> Artificial Sequence


<220> 
<223> 7141 E_xylA_G fragment

<400> 57
tgagctacac tgtccgcaag attgcgacct cgtcatggct atacggtctc gcagatcgct     60

cgcgcagatt agcgaagc                                                   78


<210> 58
<211> 79
<212> DNA
<213> Artificial Sequence


<220> 
<223> 6285 E_xylA_G fragment

<400> 58
aagggccatg accacctgat gcaccaatta ggtaggtctg gctatgtcta tacctctggc     60

gcgataccct gcgatcttc                                                  79


<210> 59
<211> 78
<212> DNA
<213> Artificial Sequence


<220> 
<223> 6273 G_xylA_D fragment

<400> 59
gccagaggta tagacatagc cagacctacc taattggtgc atcaggtggt catggccctt     60

cgcgcagatt agcgaagc                                                   78


<210> 60
<211> 79
<212> DNA
<213> Artificial Sequence


<220> 
<223> 6284 G_xylA_D fragment

<400> 60
aatcactctc catacagggt ttcatacatt tctccacggg acccacagtc gtagatgcgt     60

gcgataccct gcgatcttc                                                  79


<210> 61
<211> 79
<212> DNA
<213> Artificial Sequence


<220> 
<223> 6283 D_xylA_M fragment

<400> 61
acgcatctac gactgtgggt cccgtggaga aatgtatgaa accctgtatg gagagtgatt     60

gcgataccct gcgatcttc                                                  79


<210> 62
<211> 78
<212> DNA
<213> Artificial Sequence


<220> 
<223> 6275 D_xylA_M fragment

<400> 62
acgagagatg aaggctcacc gatggactta gtatgatgcc atgctggaag ctccggtcat     60

cgcgcagatt agcgaagc                                                   78


<210> 63
<211> 79
<212> DNA
<213> Artificial Sequence


<220> 
<223> 6287 M_xylA_N fragment

<400> 63
atgaccggag cttccagcat ggcatcatac taagtccatc ggtgagcctt catctctcgt     60

gcgataccct gcgatcttc                                                  79


<210> 64
<211> 78
<212> DNA
<213> Artificial Sequence


<220> 
<223> 6276 M_xylA_N fragment

<400> 64
ttctaggctt tgatgcaagg tccacatatc ttcgttagga ctcaatcgtg gctgctgatc     60

cgcgcagatt agcgaagc                                                   78


<210> 65
<211> 79
<212> DNA
<213> Artificial Sequence


<220> 
<223> 6288 N_xylA_O fragment

<400> 65
gatcagcagc cacgattgag tcctaacgaa gatatgtgga ccttgcatca aagcctagaa     60

gcgataccct gcgatcttc                                                  79


<210> 66
<211> 78
<212> DNA
<213> Artificial Sequence


<220> 
<223> 6277 N_xylA_O fragment

<400> 66
atactccctg cacagatgag tcaagctatt gaacaccgag aacgcgctga acgatcattc     60

cgcgcagatt agcgaagc                                                   78


<210> 67
<211> 79
<212> DNA
<213> Artificial Sequence


<220> 
<223> 6289 O_xylA_L fragment

<400> 67
gaatgatcgt tcagcgcgtt ctcggtgttc aatagcttga ctcatctgtg cagggagtat     60

gcgataccct gcgatcttc                                                  79


<210> 68
<211> 78
<212> DNA
<213> Artificial Sequence


<220> 
<223> 6627 O_xylA_L fragment

<400> 68
gccgtagctt ccgcaagtat gccgtagttg aagagcattt gccgtcggtt caggtcatat     60

cgcgcagatt agcgaagc                                                   78


<210> 69
<211> 85
<212> DNA
<213> Artificial Sequence


<220> 
<223> 7135 L_XKS1_fl fragment

<400> 69
atatgacctg aaccgacggc aaatgctctt caactacggc atacttgcgg aagctacggc     60

catagcttca aaatgtttct actcc                                           85


<210> 70
<211> 84
<212> DNA
<213> Artificial Sequence


<220> 
<223> 7134 L_XKS1_fl fragment

<400> 70
tgtggcaccg caatcattac tatggctagt gctatcattg ctgtttgacg cactgatggg     60

gtccagtgct tccacatcaa tttg                                            84


<210> 71
<211> 79
<212> DNA
<213> Artificial Sequence


<220> 
<223> 8638 PMR1 KO cassette

<400> 71
caagacgaag caaggccagc acagacgtaa gcttaagtgt aagtaaaaga taagataatt     60

cagctgaagc ttcgtacgc                                                  79


<210> 72
<211> 82
<212> DNA
<213> Artificial Sequence


<220> 
<223> 8639 PMR1 KO cassette

<400> 72
acatatgttc ccattaatta gtacatttac ctcaataggg ttgggcgtcc tcatgaaaga     60

gcataggcca ctagtggatc tg                                              82


<210> 73
<211> 20
<212> DNA
<213> Saccharomyces cerevisiae


<220> 
<223> 8640 PMR1 reintegration

<400> 73
ccatggctac tgctatttcg                                                20


<210> 74
<211> 17
<212> DNA
<213> Saccharomyces cerevisiae


<220> 
<223> 8641 PMR1 reintegration

<400> 74
agggcgttga taggatg                                                   17


<210> 75
<211> 33
<212> DNA
<213> Artificial Sequence


<220> 
<223> 5792 pUDE335 backbone

<400> 75
gttttagagc tagaaatagc aagttaaaat aag                                  33


<210> 76
<211> 17
<212> DNA
<213> Artificial Sequence


<220> 
<223> 5980 pUDE335 backbone

<400> 76
cgaccgagtt gctcttg                                                   17


<210> 77
<211> 72
<212> DNA
<213> Artificial Sequence


<220> 
<223> 5978 pUDE335 gRNA

<400> 77
attttaactt gctatttcta gctctaaaac tttatgacgt atacgtttac gatcatttat     60

ctttcactgc gg                                                         72


<210> 78
<211> 20
<212> DNA
<213> Artificial Sequence


<220> 
<223> 5979 pUDE335 gRNA

<400> 78
tattgacgcc gggcaagagc                                                20


<210> 79
<211> 18
<212> DNA
<213> Artificial Sequence


<220> 
<223> 2528 restriction analysis of gRNA

<400> 79
tctttcctgc gttatccc                                                  18


<210> 80
<211> 20
<212> DNA
<213> Artificial Sequence


<220> 
<223> 960 restriction analysis of gRNA

<400> 80
gtggatgatg tggtctctac                                                20


<210> 81
<211> 20
<212> DNA
<213> Artificial Sequence


<220> 
<223> 6640 checking PPP integration

<400> 81
ctagatgtgg tcagccattc                                                20


<210> 82
<211> 20
<212> DNA
<213> Artificial Sequence


<220> 
<223> 976 checking PPP integration

<400> 82
caccagtgtc ggcaacaacg                                                20


<210> 83
<211> 26
<212> DNA
<213> Artificial Sequence


<220> 
<223> 6717 checking PPP integration

<400> 83
ctcattagaa agaaagcata gcaatc                                         26


<210> 84
<211> 20
<212> DNA
<213> Artificial Sequence


<220> 
<223> 5603 checking PPP integration

<400> 84
cgcaagttta tcaatgtcgg                                                20


<210> 85
<211> 20
<212> DNA
<213> Artificial Sequence


<220> 
<223> 4656 checking PPP integration

<400> 85
ccttcccata tgatgctagg                                                20


<210> 86
<211> 20
<212> DNA
<213> Artificial Sequence


<220> 
<223> 7056 checking xylA integration

<400> 86
agaggtggtg gtttcgttac                                                20


<210> 87
<211> 21
<212> DNA
<213> Artificial Sequence


<220> 
<223> 6632 checking xylA integration

<400> 87
agcgtcgtag tagtggaaag c                                              21


<210> 88
<211> 20
<212> DNA
<213> Artificial Sequence


<220> 
<223> 7370 checking xylA integration

<400> 88
tgctgtaagt cgcctccatc                                                20


<210> 89
<211> 26
<212> DNA
<213> Artificial Sequence


<220> 
<223> 3293 checking xylA integration

<400> 89
gagctgaatg tatatgctgc gggatc                                         26


<210> 90
<211> 22
<212> DNA
<213> Artificial Sequence


<220> 
<223> 7369 checking xylA integration

<400> 90
agcgatctgc gagaccgtat ag                                             22


<210> 91
<211> 18
<212> DNA
<213> Artificial Sequence


<220> 
<223> 4692 checking xylA integration

<400> 91
aagggccatg accacctg                                                  18


<210> 92
<211> 19
<212> DNA
<213> Artificial Sequence


<220> 
<223> 5231 checking xylA integration

<400> 92
aatcactctc catacaggg                                                 19


<210> 93
<211> 21
<212> DNA
<213> Artificial Sequence


<220> 
<223> 3354 checking xylA integration

<400> 93
acgcatctac gactgtgggt c                                              21


<210> 94
<211> 21
<212> DNA
<213> Artificial Sequence


<220> 
<223> 4184 checking xylA integration

<400> 94
atgaccggag cttccagcat g                                              21


<210> 95
<211> 18
<212> DNA
<213> Artificial Sequence


<220> 
<223> 3843 checking xylA integration

<400> 95
gatcagcagc cacgattg                                                  18


<210> 96
<211> 18
<212> DNA
<213> Artificial Sequence


<220> 
<223> 3837 checking xylA integration

<400> 96
gaatgatcgt tcagcgcg                                                  18


<210> 97
<211> 20
<212> DNA
<213> Artificial Sequence


<220> 
<223> 6921 checking xylA integration

<400> 97
agaggtggtg gtttcgttac                                                20


<210> 98
<211> 21
<212> DNA
<213> Artificial Sequence


<220> 
<223> 3286 checking xylA integration

<400> 98
gccgtagctt ccgcaagtat g                                              21


<210> 99
<211> 20
<212> DNA
<213> Artificial Sequence


<220> 
<223> 8640 checking PMR1 KO/integration

<400> 99
ccatggctac tgctatttcg                                                20


<210> 100
<211> 17
<212> DNA
<213> Artificial Sequence


<220> 
<223> 8641 checking PMR1 KO/integration

<400> 100
agggcgttga taggatg                                                   17


<210> 101
<211> 20
<212> DNA
<213> Artificial Sequence


<220> 
<223> 9 checking PMR1 KO/integration

<400> 101
cgcacgtcaa gactgtcaag                                                20


<210> 102
<211> 20
<212> DNA
<213> Artificial Sequence


<220> 
<223> 10 checking PMR1 KO/integration

<400> 102
tcgtatgtga atgctggtcg                                                20


<210> 103
<211> 20
<212> DNA
<213> Artificial Sequence


<220> 
<223> 8792 checking PMR1 KO/integration

<400> 103
gttggactgt ctctgttagg                                                20


<210> 104
<211> 18
<212> DNA
<213> Artificial Sequence


<220> 
<223> 8793 checking PMR1 KO/integration

<400> 104
cttcgtccac ggataaag                                                  18


<210> 105
<211> 1728
<212> DNA
<213> Saccharomyces cerevisiae


<220> 
<223> Sc_SMF1 DNA sequence CEN.PK113-7D

<400> 105
atggtgaacg ttggtccttc tcatgctgca gttgctgtgg atgctagcga agcccgcaaa      60

agaaatattt cagaagaagt attcgaactg agggataaga aagattctac agtggtaatt     120

gagggtgaag ccccggtaag aacttttacc agtagctcta gtaaccatga aagagaggat     180

acgtatgttt ctaaaaggca ggtaatgaga gatatttttg ctaaatactt gaagttcatt     240

ggacctggat tgatggttag tgtggcttac atcgatcccg gtaattactc tactgccgtc     300

gatgcaggtg cctctaatca attttcccta ctttgtatca ttttgttatc aaactttatt     360

gccatatttt tgcaatgtct gtgtatcaag ttgggttccg ttacgggact agatctaagt     420

cgagcttgca gagagtattt accacggtgg ctcaactgga cattgtattt ttttgcagaa     480

tgtgccgtta tagccaccga tatagctgaa gtgattggta cagcgattgc cttgaatatc     540

ctgatcaaag tgccccttcc agcgggcgtg gccattactg ttgtggatgt gtttttgatt     600

atgtttacat ataaacctgg tgcgtcatca attaggttca ttagaatatt tgaatgtttt     660

gttgcagtat tagttgttgg cgtgtgcatt tgtttcgcaa tagaattggc ttatatcccg     720

aagagtacgt ccgttaaaca agtgttcaga ggatttgtgc catctgccca aatgtttgac     780

cacaatggta tttataccgc tatttccatc ttaggtgcta ctgttatgcc acattcgttg     840

tttttgggtt ccgctttagt gcagccaagg cttttagatt atgacgttaa acacggtaat     900

tatactgttt ctgaagaaca agataaagtg aaaaaatcta aatccactga agagattatg     960

gaagaaaaat attttaatta tagacccacg aacgctgcta tcaaatattg catgaaatat    1020

tctatggtcg aattaagcat aactctcttc accctagcgc ttttcgtcaa ttgtgccatc    1080

ctagttgttg cgggctccac tctatataac tcaccagaag cagatggggc agatttgttt    1140

actattcatg aattattatc aagaaatctg gcacccgcag caggtacgat tttcatgctc    1200

gcacttttat taagtggtca atccgcaggt gtagtgtgta ctatgtcggg tcaaattgta    1260

agtgagggtc atattaattg gaagttgcag ccatggcaaa gaagattggc cactagatgt    1320

atttcgataa tcccttgttt ggtcatctct atctgtatcg gtagagaagc tttatcaaag    1380

gccttaaatg cttcccaagt tgttttatcc atagttctgc catttttggt agcaccttta    1440

attttcttca catgtaaaaa atcaatcatg aaaaccgaaa ttaccgtcga ccatactgaa    1500

gaagatagcc ataaccatca aaataacaac gatagatctg caggtagcgt aatcgagcaa    1560

gatggttcta gtggcatgga gatagaaaat ggaaaagatg tcaaaatcgt ttatatggca    1620

aacaattgga ttatcactgt tattgctata attgtgtggc ttttcttatc tttactgaac    1680

gtttatgcca ttgttcaatt aggcatgtct catggtgata tcagttaa                 1728


<210> 106
<211> 575
<212> PRT
<213> Saccharomyces cerevisiae


<220> 
<223> Sc_Smf1 protein sequence CEN.PK113-7D

<400> 106
Met Val Asn Val Gly Pro Ser His Ala Ala Val Ala Val Asp Ala Ser 
1               5                   10                  15      
Glu Ala Arg Lys Arg Asn Ile Ser Glu Glu Val Phe Glu Leu Arg Asp 
            20                  25                  30          
Lys Lys Asp Ser Thr Val Val Ile Glu Gly Glu Ala Pro Val Arg Thr 
        35                  40                  45              
Phe Thr Ser Ser Ser Ser Asn His Glu Arg Glu Asp Thr Tyr Val Ser 
    50                  55                  60                  
Lys Arg Gln Val Met Arg Asp Ile Phe Ala Lys Tyr Leu Lys Phe Ile 
65                  70                  75                  80  
Gly Pro Gly Leu Met Val Ser Val Ala Tyr Ile Asp Pro Gly Asn Tyr 
                85                  90                  95      
Ser Thr Ala Val Asp Ala Gly Ala Ser Asn Gln Phe Ser Leu Leu Cys 
            100                 105                 110         
Ile Ile Leu Leu Ser Asn Phe Ile Ala Ile Phe Leu Gln Cys Leu Cys 
        115                 120                 125             
Ile Lys Leu Gly Ser Val Thr Gly Leu Asp Leu Ser Arg Ala Cys Arg 
    130                 135                 140                 
Glu Tyr Leu Pro Arg Trp Leu Asn Trp Thr Leu Tyr Phe Phe Ala Glu 
145                 150                 155                 160 
Cys Ala Val Ile Ala Thr Asp Ile Ala Glu Val Ile Gly Thr Ala Ile 
                165                 170                 175     
Ala Leu Asn Ile Leu Ile Lys Val Pro Leu Pro Ala Gly Val Ala Ile 
            180                 185                 190         
Thr Val Val Asp Val Phe Leu Ile Met Phe Thr Tyr Lys Pro Gly Ala 
        195                 200                 205             
Ser Ser Ile Arg Phe Ile Arg Ile Phe Glu Cys Phe Val Ala Val Leu 
    210                 215                 220                 
Val Val Gly Val Cys Ile Cys Phe Ala Ile Glu Leu Ala Tyr Ile Pro 
225                 230                 235                 240 
Lys Ser Thr Ser Val Lys Gln Val Phe Arg Gly Phe Val Pro Ser Ala 
                245                 250                 255     
Gln Met Phe Asp His Asn Gly Ile Tyr Thr Ala Ile Ser Ile Leu Gly 
            260                 265                 270         
Ala Thr Val Met Pro His Ser Leu Phe Leu Gly Ser Ala Leu Val Gln 
        275                 280                 285             
Pro Arg Leu Leu Asp Tyr Asp Val Lys His Gly Asn Tyr Thr Val Ser 
    290                 295                 300                 
Glu Glu Gln Asp Lys Val Lys Lys Ser Lys Ser Thr Glu Glu Ile Met 
305                 310                 315                 320 
Glu Glu Lys Tyr Phe Asn Tyr Arg Pro Thr Asn Ala Ala Ile Lys Tyr 
                325                 330                 335     
Cys Met Lys Tyr Ser Met Val Glu Leu Ser Ile Thr Leu Phe Thr Leu 
            340                 345                 350         
Ala Leu Phe Val Asn Cys Ala Ile Leu Val Val Ala Gly Ser Thr Leu 
        355                 360                 365             
Tyr Asn Ser Pro Glu Ala Asp Gly Ala Asp Leu Phe Thr Ile His Glu 
    370                 375                 380                 
Leu Leu Ser Arg Asn Leu Ala Pro Ala Ala Gly Thr Ile Phe Met Leu 
385                 390                 395                 400 
Ala Leu Leu Leu Ser Gly Gln Ser Ala Gly Val Val Cys Thr Met Ser 
                405                 410                 415     
Gly Gln Ile Val Ser Glu Gly His Ile Asn Trp Lys Leu Gln Pro Trp 
            420                 425                 430         
Gln Arg Arg Leu Ala Thr Arg Cys Ile Ser Ile Ile Pro Cys Leu Val 
        435                 440                 445             
Ile Ser Ile Cys Ile Gly Arg Glu Ala Leu Ser Lys Ala Leu Asn Ala 
    450                 455                 460                 
Ser Gln Val Val Leu Ser Ile Val Leu Pro Phe Leu Val Ala Pro Leu 
465                 470                 475                 480 
Ile Phe Phe Thr Cys Lys Lys Ser Ile Met Lys Thr Glu Ile Thr Val 
                485                 490                 495     
Asp His Thr Glu Glu Asp Ser His Asn His Gln Asn Asn Asn Asp Arg 
            500                 505                 510         
Ser Ala Gly Ser Val Ile Glu Gln Asp Gly Ser Ser Gly Met Glu Ile 
        515                 520                 525             
Glu Asn Gly Lys Asp Val Lys Ile Val Tyr Met Ala Asn Asn Trp Ile 
    530                 535                 540                 
Ile Thr Val Ile Ala Ile Ile Val Trp Leu Phe Leu Ser Leu Leu Asn 
545                 550                 555                 560 
Val Tyr Ala Ile Val Gln Leu Gly Met Ser His Gly Asp Ile Ser 
                565                 570                 575 

<210> 107
<211> 1650
<212> DNA
<213> Saccharomyces cerevisiae


<400> 107
atgacgtccc aagaatatga acctattcaa tggagcgatg aatcccaaac aaataatgat      60

agtgtcaacg atgcgtatgc cgacgtgaat accactcatg agtcgcgtcg aagaacaact     120

ttgcagccaa actcaacatc ccagagcatg atcggaacat tgagaaaata tgctaggttc     180

atcggtcctg gtttgatggt atctgtatcg tacatggatc ctggtaacta tagtacagct     240

gtagcagcgg gttctgctca tcggtacaag cttttatttt ctgtcttagt ttctaatttc     300

atggctgctt tttggcagta cctctgcgcc agattgggtg ctgtcactgg cctagatctg     360

gcacaaaatt gtaagaaaca tttgcccttt ggacttaaca ttactcttta tattctggcg     420

gagatggcaa ttattgccac agatctggca gaagtggtag gaaccgctat ttctttgaat     480

atccttttcc atatacctct cgccctaggt gtgatactta ctgtggtaga cgttttaatt     540

gttctgctag cttacaaacc taacggttcc atgaagggta tcagaatatt tgaagctttt     600

gtttctttat tggtggttct taccgtagtt tgctttactg ttgaactgtt ttatgccaaa     660

ctgggacctg ccaaagaaat attttctggg ttcttaccca gtaaagccgt ttttgaaggt     720

gatgggttat acttaagtct ggcaattctg ggcgccacgg taatgcctca ttcattatat     780

ttggggtcag gtgtggttca accaagatta agagagtatg atatcaaaaa cggacattac     840

ttgccagacg cgaatgatat ggataacaat cacgataatt acaggccatc ctatgaggcc     900

atcagtgaaa ctctgcactt tactataacg gaattactga tctcgctgtt cacggtggca     960

ttatttgtca attgtgccat tcttatcgtg tctggcgcta cactatacgg gtccactcaa    1020

aatgctgagg aagctgactt attttctatt tacaacttac tctgtagtac tctttccaag    1080

ggagcgggaa cggtattcgt gctggcattg ttgttttcag gacagagtgc cggtatcgtg    1140

tgtacgttga gtggacaaat ggttagtgaa gggttcttaa attggactgt ttctccggca    1200

ttgagaagat ccgccacaag agcggtagcc atcacgcctt gtttgatttt agtgcttgta    1260

gctggacgta gcggcctctc tggtgcacta aatgcttccc aagtggtact ttctctcttg    1320

ctgccttttg tttctgcacc tttactttac ttcacttcaa gcaagaagat tatgcgtgta    1380

cagcttaacc gtaccaaaga actttcaaga actacagaca aaaaacccgt ggccgaccga    1440

accgaagatg atgagaccat tgagctcgaa gaaatgggca taggcagcag ctcacaggag    1500

cgcagcttag tttctcctgc tccagaatac aaagacatga gcaatggaat gatcgtcacc    1560

gtgctcgcaa tcatagtatg gttgattatc tccggactga acttttatat gttactgggc    1620

tttactacgg gcaaagaagt acacctctaa                                     1650


<210> 108
<211> 549
<212> PRT
<213> Saccharomyces cerevisiae


<220> 
<223> Sc_Smf2 protein sequence CEN.PK113-7D

<400> 108
Met Thr Ser Gln Glu Tyr Glu Pro Ile Gln Trp Ser Asp Glu Ser Gln 
1               5                   10                  15      
Thr Asn Asn Asp Ser Val Asn Asp Ala Tyr Ala Asp Val Asn Thr Thr 
            20                  25                  30          
His Glu Ser Arg Arg Arg Thr Thr Leu Gln Pro Asn Ser Thr Ser Gln 
        35                  40                  45              
Ser Met Ile Gly Thr Leu Arg Lys Tyr Ala Arg Phe Ile Gly Pro Gly 
    50                  55                  60                  
Leu Met Val Ser Val Ser Tyr Met Asp Pro Gly Asn Tyr Ser Thr Ala 
65                  70                  75                  80  
Val Ala Ala Gly Ser Ala His Arg Tyr Lys Leu Leu Phe Ser Val Leu 
                85                  90                  95      
Val Ser Asn Phe Met Ala Ala Phe Trp Gln Tyr Leu Cys Ala Arg Leu 
            100                 105                 110         
Gly Ala Val Thr Gly Leu Asp Leu Ala Gln Asn Cys Lys Lys His Leu 
        115                 120                 125             
Pro Phe Gly Leu Asn Ile Thr Leu Tyr Ile Leu Ala Glu Met Ala Ile 
    130                 135                 140                 
Ile Ala Thr Asp Leu Ala Glu Val Val Gly Thr Ala Ile Ser Leu Asn 
145                 150                 155                 160 
Ile Leu Phe His Ile Pro Leu Ala Leu Gly Val Ile Leu Thr Val Val 
                165                 170                 175     
Asp Val Leu Ile Val Leu Leu Ala Tyr Lys Pro Asn Gly Ser Met Lys 
            180                 185                 190         
Gly Ile Arg Ile Phe Glu Ala Phe Val Ser Leu Leu Val Val Leu Thr 
        195                 200                 205             
Val Val Cys Phe Thr Val Glu Leu Phe Tyr Ala Lys Leu Gly Pro Ala 
    210                 215                 220                 
Lys Glu Ile Phe Ser Gly Phe Leu Pro Ser Lys Ala Val Phe Glu Gly 
225                 230                 235                 240 
Asp Gly Leu Tyr Leu Ser Leu Ala Ile Leu Gly Ala Thr Val Met Pro 
                245                 250                 255     
His Ser Leu Tyr Leu Gly Ser Gly Val Val Gln Pro Arg Leu Arg Glu 
            260                 265                 270         
Tyr Asp Ile Lys Asn Gly His Tyr Leu Pro Asp Ala Asn Asp Met Asp 
        275                 280                 285             
Asn Asn His Asp Asn Tyr Arg Pro Ser Tyr Glu Ala Ile Ser Glu Thr 
    290                 295                 300                 
Leu His Phe Thr Ile Thr Glu Leu Leu Ile Ser Leu Phe Thr Val Ala 
305                 310                 315                 320 
Leu Phe Val Asn Cys Ala Ile Leu Ile Val Ser Gly Ala Thr Leu Tyr 
                325                 330                 335     
Gly Ser Thr Gln Asn Ala Glu Glu Ala Asp Leu Phe Ser Ile Tyr Asn 
            340                 345                 350         
Leu Leu Cys Ser Thr Leu Ser Lys Gly Ala Gly Thr Val Phe Val Leu 
        355                 360                 365             
Ala Leu Leu Phe Ser Gly Gln Ser Ala Gly Ile Val Cys Thr Leu Ser 
    370                 375                 380                 
Gly Gln Met Val Ser Glu Gly Phe Leu Asn Trp Thr Val Ser Pro Ala 
385                 390                 395                 400 
Leu Arg Arg Ser Ala Thr Arg Ala Val Ala Ile Thr Pro Cys Leu Ile 
                405                 410                 415     
Leu Val Leu Val Ala Gly Arg Ser Gly Leu Ser Gly Ala Leu Asn Ala 
            420                 425                 430         
Ser Gln Val Val Leu Ser Leu Leu Leu Pro Phe Val Ser Ala Pro Leu 
        435                 440                 445             
Leu Tyr Phe Thr Ser Ser Lys Lys Ile Met Arg Val Gln Leu Asn Arg 
    450                 455                 460                 
Thr Lys Glu Leu Ser Arg Thr Thr Asp Lys Lys Pro Val Ala Asp Arg 
465                 470                 475                 480 
Thr Glu Asp Asp Glu Thr Ile Glu Leu Glu Glu Met Gly Ile Gly Ser 
                485                 490                 495     
Ser Ser Gln Glu Arg Ser Leu Val Ser Pro Ala Pro Glu Tyr Lys Asp 
            500                 505                 510         
Met Ser Asn Gly Met Ile Val Thr Val Leu Ala Ile Ile Val Trp Leu 
        515                 520                 525             
Ile Ile Ser Gly Leu Asn Phe Tyr Met Leu Leu Gly Phe Thr Thr Gly 
    530                 535                 540                 
Lys Glu Val His Leu 
545                 

<210> 109
<211> 824
<212> DNA
<213> Saccharomyces cerevisiae


<220> 
<223> Sc_SMF1(-1-68) DNA sequence CEN.PK113-7D

<400> 109
ctgtttctga agaacaagat aaagtgaaaa aatctaaatc cactgaagag attatggaag      60

aaaaatattt taattataga cccacgaacg ctgctatcaa atattgcatg aaatattcta     120

tggtcgaatt aagcataact ctcttcaccc tagcgctttt cgtcaattgt gccatcctag     180

ttgttgcggg ctccactcta tataactcac cagaagcaga tggggcagat ttgtttacta     240

ttcatgaatt attatcaaga aatctggcac ccgcagcagg tacgattttc atgctcgcac     300

ttttattaag tggtcaatcc gcaggtgtag tgtgtactat gtcgggtcaa attgtaagtg     360

agggtcatat taattggaag ttgcagccat ggcaaagaag attggccact agatgtattt     420

cgataatccc ttgtttggtc atctctatct gtatcggtag agaagcttta tcaaaggcct     480

taaatgcttc ccaagttgtt ttatccatag ttctgccatt tttggtagca cctttaattt     540

tcttcacatg taaaaaatca atcatgaaaa ccgaaattac cgtcgaccat actgaagaag     600

atagccataa ccatcaaaat aacaacgata gatctgcagg tagcgtaatc gagcaagatg     660

gttctagtgg catggagata gaaaatggaa aagatgtcaa aatcgtttat atggcaaaca     720

attggattat cactgttatt gctataattg tgtggctttt cttatcttta ctgaacgttt     780

atgccattgt tcaattaggc atgtctcatg gtgatatcag ttaa                      824


<210> 110
<211> 507
<212> PRT
<213> Saccharomyces cerevisiae


<220> 
<223> Sc_Smf1 (-1-68) protein sequence CEN.PK113-7D

<400> 110
Met Arg Asp Ile Phe Ala Lys Tyr Leu Lys Phe Ile Gly Pro Gly Leu 
1               5                   10                  15      
Met Val Ser Val Ala Tyr Ile Asp Pro Gly Asn Tyr Ser Thr Ala Val 
            20                  25                  30          
Asp Ala Gly Ala Ser Asn Gln Phe Ser Leu Leu Cys Ile Ile Leu Leu 
        35                  40                  45              
Ser Asn Phe Ile Ala Ile Phe Leu Gln Cys Leu Cys Ile Lys Leu Gly 
    50                  55                  60                  
Ser Val Thr Gly Leu Asp Leu Ser Arg Ala Cys Arg Glu Tyr Leu Pro 
65                  70                  75                  80  
Arg Trp Leu Asn Trp Thr Leu Tyr Phe Phe Ala Glu Cys Ala Val Ile 
                85                  90                  95      
Ala Thr Asp Ile Ala Glu Val Ile Gly Thr Ala Ile Ala Leu Asn Ile 
            100                 105                 110         
Leu Ile Lys Val Pro Leu Pro Ala Gly Val Ala Ile Thr Val Val Asp 
        115                 120                 125             
Val Phe Leu Ile Met Phe Thr Tyr Lys Pro Gly Ala Ser Ser Ile Arg 
    130                 135                 140                 
Phe Ile Arg Ile Phe Glu Cys Phe Val Ala Val Leu Val Val Gly Val 
145                 150                 155                 160 
Cys Ile Cys Phe Ala Ile Glu Leu Ala Tyr Ile Pro Lys Ser Thr Ser 
                165                 170                 175     
Val Lys Gln Val Phe Arg Gly Phe Val Pro Ser Ala Gln Met Phe Asp 
            180                 185                 190         
His Asn Gly Ile Tyr Thr Ala Ile Ser Ile Leu Gly Ala Thr Val Met 
        195                 200                 205             
Pro His Ser Leu Phe Leu Gly Ser Ala Leu Val Gln Pro Arg Leu Leu 
    210                 215                 220                 
Asp Tyr Asp Val Lys His Gly Asn Tyr Thr Val Ser Glu Glu Gln Asp 
225                 230                 235                 240 
Lys Val Lys Lys Ser Lys Ser Thr Glu Glu Ile Met Glu Glu Lys Tyr 
                245                 250                 255     
Phe Asn Tyr Arg Pro Thr Asn Ala Ala Ile Lys Tyr Cys Met Lys Tyr 
            260                 265                 270         
Ser Met Val Glu Leu Ser Ile Thr Leu Phe Thr Leu Ala Leu Phe Val 
        275                 280                 285             
Asn Cys Ala Ile Leu Val Val Ala Gly Ser Thr Leu Tyr Asn Ser Pro 
    290                 295                 300                 
Glu Ala Asp Gly Ala Asp Leu Phe Thr Ile His Glu Leu Leu Ser Arg 
305                 310                 315                 320 
Asn Leu Ala Pro Ala Ala Gly Thr Ile Phe Met Leu Ala Leu Leu Leu 
                325                 330                 335     
Ser Gly Gln Ser Ala Gly Val Val Cys Thr Met Ser Gly Gln Ile Val 
            340                 345                 350         
Ser Glu Gly His Ile Asn Trp Lys Leu Gln Pro Trp Gln Arg Arg Leu 
        355                 360                 365             
Ala Thr Arg Cys Ile Ser Ile Ile Pro Cys Leu Val Ile Ser Ile Cys 
    370                 375                 380                 
Ile Gly Arg Glu Ala Leu Ser Lys Ala Leu Asn Ala Ser Gln Val Val 
385                 390                 395                 400 
Leu Ser Ile Val Leu Pro Phe Leu Val Ala Pro Leu Ile Phe Phe Thr 
                405                 410                 415     
Cys Lys Lys Ser Ile Met Lys Thr Glu Ile Thr Val Asp His Thr Glu 
            420                 425                 430         
Glu Asp Ser His Asn His Gln Asn Asn Asn Asp Arg Ser Ala Gly Ser 
        435                 440                 445             
Val Ile Glu Gln Asp Gly Ser Ser Gly Met Glu Ile Glu Asn Gly Lys 
    450                 455                 460                 
Asp Val Lys Ile Val Tyr Met Ala Asn Asn Trp Ile Ile Thr Val Ile 
465                 470                 475                 480 
Ala Ile Ile Val Trp Leu Phe Leu Ser Leu Leu Asn Val Tyr Ala Ile 
                485                 490                 495     
Val Gln Leu Gly Met Ser His Gly Asp Ile Ser 
            500                 505         

<210> 111
<211> 1428
<212> DNA
<213> Saccharomyces cerevisiae


<220> 
<223> Sc_SMF1(K(33,34)R) DNA sequence CEN.PK113-7D

<400> 111
gatgcaggtg cctctaatca attttcccta ctttgtatca ttttgttatc aaactttatt      60

gccatatttt tgcaatgtct gtgtatcaag ttgggttccg ttacgggact agatctaagt     120

cgagcttgca gagagtattt accacggtgg ctcaactgga cattgtattt ttttgcagaa     180

tgtgccgtta tagccaccga tatagctgaa gtgattggta cagcgattgc cttgaatatc     240

ctgatcaaag tgccccttcc agcgggcgtg gccattactg ttgtggatgt gtttttgatt     300

atgtttacat ataaacctgg tgcgtcatca attaggttca ttagaatatt tgaatgtttt     360

gttgcagtat tagttgttgg cgtgtgcatt tgtttcgcaa tagaattggc ttatatcccg     420

aagagtacgt ccgttaaaca agtgttcaga ggatttgtgc catctgccca aatgtttgac     480

cacaatggta tttataccgc tatttccatc ttaggtgcta ctgttatgcc acattcgttg     540

tttttgggtt ccgctttagt gcagccaagg cttttagatt atgacgttaa acacggtaat     600

tatactgttt ctgaagaaca agataaagtg aaaaaatcta aatccactga agagattatg     660

gaagaaaaat attttaatta tagacccacg aacgctgcta tcaaatattg catgaaatat     720

tctatggtcg aattaagcat aactctcttc accctagcgc ttttcgtcaa ttgtgccatc     780

ctagttgttg cgggctccac tctatataac tcaccagaag cagatggggc agatttgttt     840

actattcatg aattattatc aagaaatctg gcacccgcag caggtacgat tttcatgctc     900

gcacttttat taagtggtca atccgcaggt gtagtgtgta ctatgtcggg tcaaattgta     960

agtgagggtc atattaattg gaagttgcag ccatggcaaa gaagattggc cactagatgt    1020

atttcgataa tcccttgttt ggtcatctct atctgtatcg gtagagaagc tttatcaaag    1080

gccttaaatg cttcccaagt tgttttatcc atagttctgc catttttggt agcaccttta    1140

attttcttca catgtaaaaa atcaatcatg aaaaccgaaa ttaccgtcga ccatactgaa    1200

gaagatagcc ataaccatca aaataacaac gatagatctg caggtagcgt aatcgagcaa    1260

gatggttcta gtggcatgga gatagaaaat ggaaaagatg tcaaaatcgt ttatatggca    1320

aacaattgga ttatcactgt tattgctata attgtgtggc ttttcttatc tttactgaac    1380

gtttatgcca ttgttcaatt aggcatgtct catggtgata tcagttaa                 1428


<210> 112
<211> 575
<212> PRT
<213> Saccharomyces cerevisiae


<220> 
<223> Sc_Smf1 (K(33,34)R) protein sequence CEN.PK113-7D

<400> 112
Met Val Asn Val Gly Pro Ser His Ala Ala Val Ala Val Asp Ala Ser 
1               5                   10                  15      
Glu Ala Arg Lys Arg Asn Ile Ser Glu Glu Val Phe Glu Leu Arg Asp 
            20                  25                  30          
Arg Arg Asp Ser Thr Val Val Ile Glu Gly Glu Ala Pro Val Arg Thr 
        35                  40                  45              
Phe Thr Ser Ser Ser Ser Asn His Glu Arg Glu Asp Thr Tyr Val Ser 
    50                  55                  60                  
Lys Arg Gln Val Met Arg Asp Ile Phe Ala Lys Tyr Leu Lys Phe Ile 
65                  70                  75                  80  
Gly Pro Gly Leu Met Val Ser Val Ala Tyr Ile Asp Pro Gly Asn Tyr 
                85                  90                  95      
Ser Thr Ala Val Asp Ala Gly Ala Ser Asn Gln Phe Ser Leu Leu Cys 
            100                 105                 110         
Ile Ile Leu Leu Ser Asn Phe Ile Ala Ile Phe Leu Gln Cys Leu Cys 
        115                 120                 125             
Ile Lys Leu Gly Ser Val Thr Gly Leu Asp Leu Ser Arg Ala Cys Arg 
    130                 135                 140                 
Glu Tyr Leu Pro Arg Trp Leu Asn Trp Thr Leu Tyr Phe Phe Ala Glu 
145                 150                 155                 160 
Cys Ala Val Ile Ala Thr Asp Ile Ala Glu Val Ile Gly Thr Ala Ile 
                165                 170                 175     
Ala Leu Asn Ile Leu Ile Lys Val Pro Leu Pro Ala Gly Val Ala Ile 
            180                 185                 190         
Thr Val Val Asp Val Phe Leu Ile Met Phe Thr Tyr Lys Pro Gly Ala 
        195                 200                 205             
Ser Ser Ile Arg Phe Ile Arg Ile Phe Glu Cys Phe Val Ala Val Leu 
    210                 215                 220                 
Val Val Gly Val Cys Ile Cys Phe Ala Ile Glu Leu Ala Tyr Ile Pro 
225                 230                 235                 240 
Lys Ser Thr Ser Val Lys Gln Val Phe Arg Gly Phe Val Pro Ser Ala 
                245                 250                 255     
Gln Met Phe Asp His Asn Gly Ile Tyr Thr Ala Ile Ser Ile Leu Gly 
            260                 265                 270         
Ala Thr Val Met Pro His Ser Leu Phe Leu Gly Ser Ala Leu Val Gln 
        275                 280                 285             
Pro Arg Leu Leu Asp Tyr Asp Val Lys His Gly Asn Tyr Thr Val Ser 
    290                 295                 300                 
Glu Glu Gln Asp Lys Val Lys Lys Ser Lys Ser Thr Glu Glu Ile Met 
305                 310                 315                 320 
Glu Glu Lys Tyr Phe Asn Tyr Arg Pro Thr Asn Ala Ala Ile Lys Tyr 
                325                 330                 335     
Cys Met Lys Tyr Ser Met Val Glu Leu Ser Ile Thr Leu Phe Thr Leu 
            340                 345                 350         
Ala Leu Phe Val Asn Cys Ala Ile Leu Val Val Ala Gly Ser Thr Leu 
        355                 360                 365             
Tyr Asn Ser Pro Glu Ala Asp Gly Ala Asp Leu Phe Thr Ile His Glu 
    370                 375                 380                 
Leu Leu Ser Arg Asn Leu Ala Pro Ala Ala Gly Thr Ile Phe Met Leu 
385                 390                 395                 400 
Ala Leu Leu Leu Ser Gly Gln Ser Ala Gly Val Val Cys Thr Met Ser 
                405                 410                 415     
Gly Gln Ile Val Ser Glu Gly His Ile Asn Trp Lys Leu Gln Pro Trp 
            420                 425                 430         
Gln Arg Arg Leu Ala Thr Arg Cys Ile Ser Ile Ile Pro Cys Leu Val 
        435                 440                 445             
Ile Ser Ile Cys Ile Gly Arg Glu Ala Leu Ser Lys Ala Leu Asn Ala 
    450                 455                 460                 
Ser Gln Val Val Leu Ser Ile Val Leu Pro Phe Leu Val Ala Pro Leu 
465                 470                 475                 480 
Ile Phe Phe Thr Cys Lys Lys Ser Ile Met Lys Thr Glu Ile Thr Val 
                485                 490                 495     
Asp His Thr Glu Glu Asp Ser His Asn His Gln Asn Asn Asn Asp Arg 
            500                 505                 510         
Ser Ala Gly Ser Val Ile Glu Gln Asp Gly Ser Ser Gly Met Glu Ile 
        515                 520                 525             
Glu Asn Gly Lys Asp Val Lys Ile Val Tyr Met Ala Asn Asn Trp Ile 
    530                 535                 540                 
Ile Thr Val Ile Ala Ile Ile Val Trp Leu Phe Leu Ser Leu Leu Asn 
545                 550                 555                 560 
Val Tyr Ala Ile Val Gln Leu Gly Met Ser His Gly Asp Ile Ser 
                565                 570                 575 

<210> 113
<211> 1728
<212> DNA
<213> Saccharomyces cerevisiae


<220> 
<223> Sc_SMF1 (K(20,33,34,65)R) DNA sequence CEN.PK113-7D

<400> 113
atggtgaacg ttggtccttc tcatgctgca gttgctgtgg atgctagcga agcccgcaga      60

agaaatattt cagaagaagt attcgaactg agggatagaa gagattctac agtggtaatt     120

gagggtgaag ccccggtaag aacttttacc agtagctcta gtaaccatga aagagaggat     180

acgtatgttt ctagaaggca ggtaatgaga gatatttttg ctaaatactt gaagttcatt     240

ggacctggat tgatggttag tgtggcttac atcgatcccg gtaattactc tactgccgtc     300

gatgcaggtg cctctaatca attttcccta ctttgtatca ttttgttatc aaactttatt     360

gccatatttt tgcaatgtct gtgtatcaag ttgggttccg ttacgggact agatctaagt     420

cgagcttgca gagagtattt accacggtgg ctcaactgga cattgtattt ttttgcagaa     480

tgtgccgtta tagccaccga tatagctgaa gtgattggta cagcgattgc cttgaatatc     540

ctgatcaaag tgccccttcc agcgggcgtg gccattactg ttgtggatgt gtttttgatt     600

atgtttacat ataaacctgg tgcgtcatca attaggttca ttagaatatt tgaatgtttt     660

gttgcagtat tagttgttgg cgtgtgcatt tgtttcgcaa tagaattggc ttatatcccg     720

aagagtacgt ccgttaaaca agtgttcaga ggatttgtgc catctgccca aatgtttgac     780

cacaatggta tttataccgc tatttccatc ttaggtgcta ctgttatgcc acattcgttg     840

tttttgggtt ccgctttagt gcagccaagg cttttagatt atgacgttaa acacggtaat     900

tatactgttt ctgaagaaca agataaagtg aaaaaatcta aatccactga agagattatg     960

gaagaaaaat attttaatta tagacccacg aacgctgcta tcaaatattg catgaaatat    1020

tctatggtcg aattaagcat aactctcttc accctagcgc ttttcgtcaa ttgtgccatc    1080

ctagttgttg cgggctccac tctatataac tcaccagaag cagatggggc agatttgttt    1140

actattcatg aattattatc aagaaatctg gcacccgcag caggtacgat tttcatgctc    1200

gcacttttat taagtggtca atccgcaggt gtagtgtgta ctatgtcggg tcaaattgta    1260

agtgagggtc atattaattg gaagttgcag ccatggcaaa gaagattggc cactagatgt    1320

atttcgataa tcccttgttt ggtcatctct atctgtatcg gtagagaagc tttatcaaag    1380

gccttaaatg cttcccaagt tgttttatcc atagttctgc catttttggt agcaccttta    1440

attttcttca catgtaaaaa atcaatcatg aaaaccgaaa ttaccgtcga ccatactgaa    1500

gaagatagcc ataaccatca aaataacaac gatagatctg caggtagcgt aatcgagcaa    1560

gatggttcta gtggcatgga gatagaaaat ggaaaagatg tcaaaatcgt ttatatggca    1620

aacaattgga ttatcactgt tattgctata attgtgtggc ttttcttatc tttactgaac    1680

gtttatgcca ttgttcaatt aggcatgtct catggtgata tcagttaa                 1728


<210> 114
<211> 575
<212> PRT
<213> Saccharomyces cerevisiae


<220> 
<223> Sc_Smf1 (K(20,33,34,65)R) protein sequence CEN.PK113-7D

<400> 114
Met Val Asn Val Gly Pro Ser His Ala Ala Val Ala Val Asp Ala Ser 
1               5                   10                  15      
Glu Ala Arg Arg Arg Asn Ile Ser Glu Glu Val Phe Glu Leu Arg Asp 
            20                  25                  30          
Arg Arg Asp Ser Thr Val Val Ile Glu Gly Glu Ala Pro Val Arg Thr 
        35                  40                  45              
Phe Thr Ser Ser Ser Ser Asn His Glu Arg Glu Asp Thr Tyr Val Ser 
    50                  55                  60                  
Arg Arg Gln Val Met Arg Asp Ile Phe Ala Lys Tyr Leu Lys Phe Ile 
65                  70                  75                  80  
Gly Pro Gly Leu Met Val Ser Val Ala Tyr Ile Asp Pro Gly Asn Tyr 
                85                  90                  95      
Ser Thr Ala Val Asp Ala Gly Ala Ser Asn Gln Phe Ser Leu Leu Cys 
            100                 105                 110         
Ile Ile Leu Leu Ser Asn Phe Ile Ala Ile Phe Leu Gln Cys Leu Cys 
        115                 120                 125             
Ile Lys Leu Gly Ser Val Thr Gly Leu Asp Leu Ser Arg Ala Cys Arg 
    130                 135                 140                 
Glu Tyr Leu Pro Arg Trp Leu Asn Trp Thr Leu Tyr Phe Phe Ala Glu 
145                 150                 155                 160 
Cys Ala Val Ile Ala Thr Asp Ile Ala Glu Val Ile Gly Thr Ala Ile 
                165                 170                 175     
Ala Leu Asn Ile Leu Ile Lys Val Pro Leu Pro Ala Gly Val Ala Ile 
            180                 185                 190         
Thr Val Val Asp Val Phe Leu Ile Met Phe Thr Tyr Lys Pro Gly Ala 
        195                 200                 205             
Ser Ser Ile Arg Phe Ile Arg Ile Phe Glu Cys Phe Val Ala Val Leu 
    210                 215                 220                 
Val Val Gly Val Cys Ile Cys Phe Ala Ile Glu Leu Ala Tyr Ile Pro 
225                 230                 235                 240 
Lys Ser Thr Ser Val Lys Gln Val Phe Arg Gly Phe Val Pro Ser Ala 
                245                 250                 255     
Gln Met Phe Asp His Asn Gly Ile Tyr Thr Ala Ile Ser Ile Leu Gly 
            260                 265                 270         
Ala Thr Val Met Pro His Ser Leu Phe Leu Gly Ser Ala Leu Val Gln 
        275                 280                 285             
Pro Arg Leu Leu Asp Tyr Asp Val Lys His Gly Asn Tyr Thr Val Ser 
    290                 295                 300                 
Glu Glu Gln Asp Lys Val Lys Lys Ser Lys Ser Thr Glu Glu Ile Met 
305                 310                 315                 320 
Glu Glu Lys Tyr Phe Asn Tyr Arg Pro Thr Asn Ala Ala Ile Lys Tyr 
                325                 330                 335     
Cys Met Lys Tyr Ser Met Val Glu Leu Ser Ile Thr Leu Phe Thr Leu 
            340                 345                 350         
Ala Leu Phe Val Asn Cys Ala Ile Leu Val Val Ala Gly Ser Thr Leu 
        355                 360                 365             
Tyr Asn Ser Pro Glu Ala Asp Gly Ala Asp Leu Phe Thr Ile His Glu 
    370                 375                 380                 
Leu Leu Ser Arg Asn Leu Ala Pro Ala Ala Gly Thr Ile Phe Met Leu 
385                 390                 395                 400 
Ala Leu Leu Leu Ser Gly Gln Ser Ala Gly Val Val Cys Thr Met Ser 
                405                 410                 415     
Gly Gln Ile Val Ser Glu Gly His Ile Asn Trp Lys Leu Gln Pro Trp 
            420                 425                 430         
Gln Arg Arg Leu Ala Thr Arg Cys Ile Ser Ile Ile Pro Cys Leu Val 
        435                 440                 445             
Ile Ser Ile Cys Ile Gly Arg Glu Ala Leu Ser Lys Ala Leu Asn Ala 
    450                 455                 460                 
Ser Gln Val Val Leu Ser Ile Val Leu Pro Phe Leu Val Ala Pro Leu 
465                 470                 475                 480 
Ile Phe Phe Thr Cys Lys Lys Ser Ile Met Lys Thr Glu Ile Thr Val 
                485                 490                 495     
Asp His Thr Glu Glu Asp Ser His Asn His Gln Asn Asn Asn Asp Arg 
            500                 505                 510         
Ser Ala Gly Ser Val Ile Glu Gln Asp Gly Ser Ser Gly Met Glu Ile 
        515                 520                 525             
Glu Asn Gly Lys Asp Val Lys Ile Val Tyr Met Ala Asn Asn Trp Ile 
    530                 535                 540                 
Ile Thr Val Ile Ala Ile Ile Val Trp Leu Phe Leu Ser Leu Leu Asn 
545                 550                 555                 560 
Val Tyr Ala Ile Val Gln Leu Gly Met Ser His Gly Asp Ile Ser 
                565                 570                 575 

<210> 115
<211> 1764
<212> DNA
<213> Saccharomyces cerevisiae


<220> 
<223> Sc_PHO84 DNA sequence CEN.PK113-7D

<400> 115
atgagttccg tcaataaaga tactattcat gttgctgaaa gaagtcttca taaagaacac      60

cttaccgaag gtggtaacat ggccttccac aaccatttga atgattttgc tcatattgaa     120

gatcctctgg aaagaagaag attggctttg gagtccatcg atgacgaagg tttcggttgg     180

caacaagtta agaccatctc cattgctggt gttggtttct tgacagattc ttatgatatt     240

tttgccatta atttgggtat cactatgatg tcctacgttt actggcacgg tagtatgcca     300

ggtccaagtc aaaccttgtt gaaggtttcc acttctgttg gtactgttat tggtcaattt     360

ggttttggta ctttagctga tattgttggt cgtaagagaa tttatggtat ggaacttatt     420

atcatgattg tctgtaccat tctgcaaacc actgttgctc attctcctgc tattaacttc     480

gttgctgttt taacattcta ccgtattgtc atgggtattg gtatcggtgg tgactaccca     540

ctatcttcta ttattacttc tgaatttgcc actaccaaat ggagaggtgc catcatgggt     600

gctgtctttg ctaaccaagc ttggggtcaa atctccggtg gtatcatcgc tcttatcttg     660

gttgctgctt acaagggcga actagaatac gcaaactctg gtgctgaatg tgatgctaga     720

tgtcaaaagg cttgtgacca aatgtggaga atccttattg ggttgggtac cgttctaggg     780

ttggcatgtt tgtatttcag attaactatt ccagaatctc ctagatatca attggatgtt     840

aacgctaagt tggaacttgc tgctgccgca caagaacaag atggcgaaaa gaaaattcac     900

gacaccagtg atgaagacat ggcaattaac ggtttggaaa gagcttctac tgccgtcgaa     960

tctcttgaca atcatcctcc aaaggcttcg ttcaaagatt tctgcagaca ttttggtcaa    1020

tggaagtacg gtaagatttt gctaggtact gctggttcat ggtttacctt agatgttgct    1080

ttctacgggt tgagtttaaa cagtgctgtt attctgcaaa ccatcggtta tgccggttcc    1140

aaaaacgttt acaagaaact gtatgatact gctgtcggta atctgatttt gatttgtgct    1200

ggttcattac ctggttactg ggtatccgtc ttcactgtcg atataatcgg tagaaaacca    1260

attcaattag ccggtttcat catcttgacc gctttgttct gtgtcatcgg tttcgcatac    1320

cataaacttg gtgaccatgg tctgttggct ctttacgtca tttgtcaatt cttccaaaac    1380

ttcggtccaa acacaaccac ctttattgtt cctggtgagt gtttcccaac tcgttacaga    1440

tctactgctc atggtatttc tgctgcatct ggtaaggtcg gtgccattat tgcacaaacc    1500

gctttgggta ctctaatcga ccataactgt gctagagacg gtaagccaac caactgttgg    1560

ttacctcacg tcatggaaat tttcgcctta ttcatgttgt tgggtatctt cacaaccttg    1620

ttgatcccag aaactaagag aaagactcta gaagaaatta acgagctata ccacgatgaa    1680

atcgatcctg ctacgctaaa cttcagaaac aagaataatg acattgaatc ttccagccca    1740

tctcaacttc aacatgaagc ataa                                           1764


<210> 116
<211> 587
<212> PRT
<213> Saccharomyces cerevisiae


<220> 
<223> Sc_Pho84 protein sequence CEN.PK113-7D

<400> 116
Met Ser Ser Val Asn Lys Asp Thr Ile His Val Ala Glu Arg Ser Leu 
1               5                   10                  15      
His Lys Glu His Leu Thr Glu Gly Gly Asn Met Ala Phe His Asn His 
            20                  25                  30          
Leu Asn Asp Phe Ala His Ile Glu Asp Pro Leu Glu Arg Arg Arg Leu 
        35                  40                  45              
Ala Leu Glu Ser Ile Asp Asp Glu Gly Phe Gly Trp Gln Gln Val Lys 
    50                  55                  60                  
Thr Ile Ser Ile Ala Gly Val Gly Phe Leu Thr Asp Ser Tyr Asp Ile 
65                  70                  75                  80  
Phe Ala Ile Asn Leu Gly Ile Thr Met Met Ser Tyr Val Tyr Trp His 
                85                  90                  95      
Gly Ser Met Pro Gly Pro Ser Gln Thr Leu Leu Lys Val Ser Thr Ser 
            100                 105                 110         
Val Gly Thr Val Ile Gly Gln Phe Gly Phe Gly Thr Leu Ala Asp Ile 
        115                 120                 125             
Val Gly Arg Lys Arg Ile Tyr Gly Met Glu Leu Ile Ile Met Ile Val 
    130                 135                 140                 
Cys Thr Ile Leu Gln Thr Thr Val Ala His Ser Pro Ala Ile Asn Phe 
145                 150                 155                 160 
Val Ala Val Leu Thr Phe Tyr Arg Ile Val Met Gly Ile Gly Ile Gly 
                165                 170                 175     
Gly Asp Tyr Pro Leu Ser Ser Ile Ile Thr Ser Glu Phe Ala Thr Thr 
            180                 185                 190         
Lys Trp Arg Gly Ala Ile Met Gly Ala Val Phe Ala Asn Gln Ala Trp 
        195                 200                 205             
Gly Gln Ile Ser Gly Gly Ile Ile Ala Leu Ile Leu Val Ala Ala Tyr 
    210                 215                 220                 
Lys Gly Glu Leu Glu Tyr Ala Asn Ser Gly Ala Glu Cys Asp Ala Arg 
225                 230                 235                 240 
Cys Gln Lys Ala Cys Asp Gln Met Trp Arg Ile Leu Ile Gly Leu Gly 
                245                 250                 255     
Thr Val Leu Gly Leu Ala Cys Leu Tyr Phe Arg Leu Thr Ile Pro Glu 
            260                 265                 270         
Ser Pro Arg Tyr Gln Leu Asp Val Asn Ala Lys Leu Glu Leu Ala Ala 
        275                 280                 285             
Ala Ala Gln Glu Gln Asp Gly Glu Lys Lys Ile His Asp Thr Ser Asp 
    290                 295                 300                 
Glu Asp Met Ala Ile Asn Gly Leu Glu Arg Ala Ser Thr Ala Val Glu 
305                 310                 315                 320 
Ser Leu Asp Asn His Pro Pro Lys Ala Ser Phe Lys Asp Phe Cys Arg 
                325                 330                 335     
His Phe Gly Gln Trp Lys Tyr Gly Lys Ile Leu Leu Gly Thr Ala Gly 
            340                 345                 350         
Ser Trp Phe Thr Leu Asp Val Ala Phe Tyr Gly Leu Ser Leu Asn Ser 
        355                 360                 365             
Ala Val Ile Leu Gln Thr Ile Gly Tyr Ala Gly Ser Lys Asn Val Tyr 
    370                 375                 380                 
Lys Lys Leu Tyr Asp Thr Ala Val Gly Asn Leu Ile Leu Ile Cys Ala 
385                 390                 395                 400 
Gly Ser Leu Pro Gly Tyr Trp Val Ser Val Phe Thr Val Asp Ile Ile 
                405                 410                 415     
Gly Arg Lys Pro Ile Gln Leu Ala Gly Phe Ile Ile Leu Thr Ala Leu 
            420                 425                 430         
Phe Cys Val Ile Gly Phe Ala Tyr His Lys Leu Gly Asp His Gly Leu 
        435                 440                 445             
Leu Ala Leu Tyr Val Ile Cys Gln Phe Phe Gln Asn Phe Gly Pro Asn 
    450                 455                 460                 
Thr Thr Thr Phe Ile Val Pro Gly Glu Cys Phe Pro Thr Arg Tyr Arg 
465                 470                 475                 480 
Ser Thr Ala His Gly Ile Ser Ala Ala Ser Gly Lys Val Gly Ala Ile 
                485                 490                 495     
Ile Ala Gln Thr Ala Leu Gly Thr Leu Ile Asp His Asn Cys Ala Arg 
            500                 505                 510         
Asp Gly Lys Pro Thr Asn Cys Trp Leu Pro His Val Met Glu Ile Phe 
        515                 520                 525             
Ala Leu Phe Met Leu Leu Gly Ile Phe Thr Thr Leu Leu Ile Pro Glu 
    530                 535                 540                 
Thr Lys Arg Lys Thr Leu Glu Glu Ile Asn Glu Leu Tyr His Asp Glu 
545                 550                 555                 560 
Ile Asp Pro Ala Thr Leu Asn Phe Arg Asn Lys Asn Asn Asp Ile Glu 
                565                 570                 575     
Ser Ser Ser Pro Ser Gln Leu Gln His Glu Ala 
            580                 585         

<210> 117
<211> 942
<212> DNA
<213> Saccharomyces cerevisiae


<220> 
<223> Sc_ATX2 DNA sequence CEN.PK113-7D

<400> 117
atgaagtttt tgggtgtaat actgctagca agttttcttt tgattgctac cttcctcata      60

gggcttatcc cattgtatta tattgataaa caaaaaagtt ccattgtcac aaaccaagaa     120

ggtgctgact caatttctga ttttactacg aacgcagata ctcaaacgat caatgacgat     180

gttagtagtt accgtgtgaa gattgccgtt ctatcacaat tcggtatagg aatgttgtta     240

ggtacatcat ttatgttggt aataccggaa ggtatcaaag cgtgtgtcga acatgatgga     300

aacgttggtg tgaatttatt aattgggttt ctaggtgttt atgttctcga taggttggtt     360

acactatggg tttctagaaa gcaaacggtc tatacgcatg atgctgtcaa atttcaaagc     420

tggaaagata taataaacca tccaagacaa atatggatga acttaataca aaacaatgtt     480

gtctttgcct tattcattca tggactatct gatggaattg cattaggtac gacaacgaac     540

aatgattcac tgctcatagt ggtgttaatt gctatagtta ttcataaaat tcctgccgtt     600

ctttccctca ctagtttgat ggtatcgaga caaaacttaa tgaaatggga ggtgatctgt     660

aacgttttct tgtttgcgtc atcgactccg atcggataca tagtgttatc ccttttgaat     720

ctgagtcatt cgccaacgat ggattggata agtggaaatc ttctgcttat gagtggtggc     780

agtttactat atgcgtcttt cacagcattt gttggtggag attctcatga tcacgatctc     840

agcgttgaac aggaagtcgt tttacctcat gatgaatccg tttatgtttt gataggtgtc     900

tgcataccgt tggtgatatc gtactgcatc tctgaagaat aa                        942


<210> 118
<211> 313
<212> PRT
<213> Saccharomyces cerevisiae


<220> 
<223> Sc_Atx2 protein sequence CEN.PK113-7D

<400> 118
Met Lys Phe Leu Gly Val Ile Leu Leu Ala Ser Phe Leu Leu Ile Ala 
1               5                   10                  15      
Thr Phe Leu Ile Gly Leu Ile Pro Leu Tyr Tyr Ile Asp Lys Gln Lys 
            20                  25                  30          
Ser Ser Ile Val Thr Asn Gln Glu Gly Ala Asp Ser Ile Ser Asp Phe 
        35                  40                  45              
Thr Thr Asn Ala Asp Thr Gln Thr Ile Asn Asp Asp Val Ser Ser Tyr 
    50                  55                  60                  
Arg Val Lys Ile Ala Val Leu Ser Gln Phe Gly Ile Gly Met Leu Leu 
65                  70                  75                  80  
Gly Thr Ser Phe Met Leu Val Ile Pro Glu Gly Ile Lys Ala Cys Val 
                85                  90                  95      
Glu His Asp Gly Asn Val Gly Val Asn Leu Leu Ile Gly Phe Leu Gly 
            100                 105                 110         
Val Tyr Val Leu Asp Arg Leu Val Thr Leu Trp Val Ser Arg Lys Gln 
        115                 120                 125             
Thr Val Tyr Thr His Asp Ala Val Lys Phe Gln Ser Trp Lys Asp Ile 
    130                 135                 140                 
Ile Asn His Pro Arg Gln Ile Trp Met Asn Leu Ile Gln Asn Asn Val 
145                 150                 155                 160 
Val Phe Ala Leu Phe Ile His Gly Leu Ser Asp Gly Ile Ala Leu Gly 
                165                 170                 175     
Thr Thr Thr Asn Asn Asp Ser Leu Leu Ile Val Val Leu Ile Ala Ile 
            180                 185                 190         
Val Ile His Lys Ile Pro Ala Val Leu Ser Leu Thr Ser Leu Met Val 
        195                 200                 205             
Ser Arg Gln Asn Leu Met Lys Trp Glu Val Ile Cys Asn Val Phe Leu 
    210                 215                 220                 
Phe Ala Ser Ser Thr Pro Ile Gly Tyr Ile Val Leu Ser Leu Leu Asn 
225                 230                 235                 240 
Leu Ser His Ser Pro Thr Met Asp Trp Ile Ser Gly Asn Leu Leu Leu 
                245                 250                 255     
Met Ser Gly Gly Ser Leu Leu Tyr Ala Ser Phe Thr Ala Phe Val Gly 
            260                 265                 270         
Gly Asp Ser His Asp His Asp Leu Ser Val Glu Gln Glu Val Val Leu 
        275                 280                 285             
Pro His Asp Glu Ser Val Tyr Val Leu Ile Gly Val Cys Ile Pro Leu 
    290                 295                 300                 
Val Ile Ser Tyr Cys Ile Ser Glu Glu 
305                 310             

<210> 119
<211> 600
<212> DNA
<213> Saccharomyces cerevisiae


<220> 
<223> Sc_TPI1 promoter

<400> 119
gacacctaac tacatagtgt ttaaagatta cggatattta acttacttag aataatgcca      60

tttttttgag ttataataat cctacgttag tgtgagcggg atttaaactg tgaggacctt     120

aatacattca gacacttctg cggtatcacc ctacttattc ccttcgagat tatatctagg     180

aacccatcag gttggtggaa gattacccgt tctaagactt ttcagcttcc tctattgatg     240

ttacacctgg acaccccttt tctggcatcc agtttttaat cttcagtggc atgtgagatt     300

ctccgaaatt aattaaagca atcacacaat tctctcggat accacctcgg ttgaaactga     360

caggtggttt gttacgcatg ctaatgcaaa ggagcctata tacctttggc tcggctgctg     420

taacagggaa tataaagggc agcataattt aggagtttag tgaacttgca acatttacta     480

ttttcccttc ttacgtaaat atttttcttt ttaattctaa atcaatcttt ttcaattttt     540

tgtttgtatt cttttcttgc ttaaatctat aactacaaaa aacacataca taaactaaaa     600


<210> 120
<211> 301
<212> DNA
<213> Saccharomyces cerevisiae


<220> 
<223> Sc_PGK1 terminator

<400> 120
aattgaattg aattgaaatc gatagatcaa tttttttctt ttctctttcc ccatccttta     60

cgctaaaata atagtttatt ttattttttg aatatttttt atttatatac gtatatatag    120

actattattt atcttttaat gattattaag atttttatta aaaaaaaatt cgctcctctt    180

ttaatgcctt tatgcagttt ttttttccca ttcgatattt ctatgttcgg gttcagcgta    240

ttttaagttt aataactcga aaattctgcg ttcgttaaag ctttcgagaa ggatattatt    300

t                                                                    301


<210> 121
<211> 486
<212> DNA
<213> Artificial Sequence


<220> 
<223> gBLOCK bearing sequence of SNR52p-gRNA.INT1-SUP4t

<400> 121
ctgcagcccg ggggatccac tagttctaga gcggccgcca ccgcccgcgg tctttgaaaa      60

gataatgtat gattatgctt tcactcatat ttatacagaa acttgatgtt ttctttcgag     120

tatatacaag gtgattacat gtacgtttga agtacaactc tagattttgt agtgccctct     180

tgggctagcg gtaaaggtgc gcattttttc acaccctaca atgttctgtt caaaagattt     240

tggtcaaacg ctgtagaagt gaaagttggt gcgcatgttt cggcgttcga aacttctccg     300

cagtgaaaga taaatgatct attagaacca gggaggtccg ttttagagct agaaatagca     360

agttaaaata aggctagtcc gttatcaact tgaaaaagtg gcaccgagtc ggtggtgctt     420

tttttgtttt ttatgtctcc gcggcaatta accctcacta aagggaacaa aagctggagc     480

tccacc                                                                486


<210> 122
<211> 699
<212> DNA
<213> Artificial Sequence


<220> 
<223> pRN599

<400> 122
gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtga aaaaaagcgg      60

ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca     120

tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg     180

tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct     240

cttgcccggc gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca     300

tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca     360

gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg     420

tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac     480

ggaaatgttg aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt     540

attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc     600

cgcgcacatt tccccgaaaa gtgccacctg acgtctaaga aaccattatt atcatgacat     660

taacctataa aaataggcgt atcacgaggc cctttcgtc                            699


<210> 123
<211> 19
<212> DNA
<213> Artificial Sequence


<220> 
<223> Forward primer INT1-5'flank (BOZ-783)

<400> 123
cggcattatt gtgtatggc                                                 19


<210> 124
<211> 72
<212> DNA
<213> Artificial Sequence


<220> 
<223> reverse primer INT1-5 'flank with connector c (DBC-19944)

<400> 124
caacaggagg cggatggata tactgtggtc tggaagatgc cggaaagcgt catgttatgc     60

tcctcaatca cg                                                         72


<210> 125
<211> 21
<212> DNA
<213> Artificial Sequence


<220> 
<223> forward primer connector c to amplify anaerobic promoter-PRK-PGK1
      terminator expression cassette (DBC-5799)

<400> 125
acgctttccg gcatcttcca g                                              21


<210> 126
<211> 20
<212> DNA
<213> Artificial Sequence


<220> 
<223> reverse primer connector d to amplify PRK expression cassette
      (DBC-5800)

<400> 126
gcggaatatt ggcggaacgg                                                20


<210> 127
<211> 74
<212> DNA
<213> Artificial Sequence


<220> 
<223> forward primer to amplify URA3 marker with flank connector d
      (DBC-19947)

<400> 127
aacgttgtcc aggtttgtat ccacgtgtgt ccgttccgcc aatattccgc gctgtggttt     60

cagggtccat aaag                                                       74


<210> 128
<211> 72
<212> DNA
<213> Artificial Sequence


<220> 
<223> reverse primer to amplify URA3 locus with flank connector e
      (DBC-19949)

<400> 128
aggtacaaca agcacgaccg agcatatggg aaggatgttt gtggttattt cgagattccc     60

gggtaataac tg                                                         72


<210> 129
<211> 20
<212> DNA
<213> Artificial Sequence


<220> 
<223> forward primer INT1-3'flank with connector d (DBC-19946)

<400> 129
aactccatca aatggtcagg                                                20


<210> 130
<211> 20
<212> DNA
<213> Artificial Sequence


<220> 
<223> reverse primer INT1-3 'flank (BoZ-788)

<400> 130
aacaaaagct gggtaccggg                                                20


<210> 131
<211> 73
<212> DNA
<213> Artificial Sequence


<220> 
<223> forward primer on backbone pRN599 (DBC-13775)

<400> 131
cttatcgata ccgtcgacct cgaggggggg cccggtaccc agcttttgtt ccgcggtctt     60

tgaaaagata atg                                                        73


<210> 132
<211> 69
<212> DNA
<213> Artificial Sequence


<220> 
<223> reverse primer on backbone pRN599 (DBC-13776)

<400> 132
aaaatacaac aaataaaaaa cactcaatga cctgaccatt tgatggagtt ccgcggagac     60

ataaaaaac                                                             69


<210> 133
<211> 73
<212> DNA
<213> Artificial Sequence


<220> 
<223> forward primer on gRNA cassette INT1 (DBC-13773)

<400> 133
cttatcgata ccgtcgacct cgaggggggg cccggtaccc agcttttgtt ccgcggtctt     60

tgaaaagata atg                                                        73


<210> 134
<211> 69
<212> DNA
<213> Artificial Sequence


<220> 
<223> reverse primer on gRNa cassette INT1 (DBC-13774)

<400> 134
aaaatacaac aaataaaaaa cactcaatga cctgaccatt tgatggagtt ccgcggagac     60

ataaaaaac                                                             69


<210> 135
<211> 1903
<212> DNA
<213> Saccharomyces cerevisiae


<220> 
<223> Sc_ PMR1 DNA sequence of CEN.PK113-7D

<400> 135
aatatcggta tccttagcgg ttgctgctat tccagaaggg ttaccaatta ttgtcactgt      60

tactttggca ttgggtgttc tgagaatggc caagcgtaaa gccatcgtga gaaggttacc     120

aagtgtcgaa actttaggct ctgtcaacgt tatctgctcc gacaaaacag gtacactaac     180

ctcaaaccac atgaccgtat ctaaactttg gtgcttggac agtatgtcca ataagctaaa     240

cgtcctctca ttagacaaaa ataagaagac taaaaattct aatggaaatt tgaaaaacta     300

tttgactgaa gacgttaggg aaactctaac tatcggtaat ctctgtaata atgcatcttt     360

ctctcaagaa catgccatat ttctgggaaa tcctactgat gtagctcttt tagagcaatt     420

ggcaaacttt gaaatgcctg atatcagaaa caccgttcaa aaagttcagg aacttccatt     480

taactcgaaa agaaaattaa tggcaaccaa gattctcaac cctgtcgaca ataagtgtac     540

agtttatgtt aaaggtgcat ttgaaagaat tcttgagtac tccacaagtt atttgaaatc     600

aaagggtaaa aaaactgaaa agttgactga agcccaaaaa gctacgataa atgagtgcgc     660

aaattctatg gcatctgaag gtttgcgtgt ctttggattt gctaaactaa ctttgtctga     720

ttcatcaact cctctaaccg aagacctaat caaagattta acctttactg gtttaatcgg     780

tatgaatgac ccaccaagac cgaacgttaa atttgccatc gaacaattac tacaaggtgg     840

tgtccatatt attatgatca ctggtgattc tgagaatacc gcagtaaaca ttgcaaaaca     900

aattggtatt ccagttattg atccaaagct ttccgtttta tccggtgata aattagatga     960

aatgtcagat gatcaactgg ccaatgtcat cgaccacgtt aatatttttg ctcgtgctac    1020

gcctgagcat aagttaaaca ttgttcgtgc attaagaaag aggggtgatg tggtagcaat    1080

gactggtgat ggtgttaacg acgctcctgc gttgaaactt tcagatattg gtgtttctat    1140

gggtagaatt ggtacagatg tagccaaaga agcctcagat atggtcttaa ctgatgatga    1200

cttcagtact attttaactg ccattgaaga gggtaaaggt atctttaata atattcagaa    1260

tttcctgact tttcaattgt ctacttctgt tgccgcacta tcattagttg cactatctac    1320

agcgtttaaa ctacccaatc cactgaacgc aatgcaaatt ctttggataa atattttaat    1380

ggatgggcca ccagctcaat ccttaggtgt ggaacctgtt gatcatgaag ttatgaaaaa    1440

acctccaaga aaacgtaccg ataaaatttt gacccatgat gtaatgaaac gtttactaac    1500

caccgcggcc tgtatcatcg ttgggacagt ttacattttt gttaaagaga tggccgaaga    1560

tggtaaagta actgctagag atactactat gacatttact tgttttgttt tttttgatat    1620

gtttaatgct ttggcctgca gacataacac aaagtcaatc ttcgaaatcg gctttttcac    1680

gaacaaaatg ttcaactacg ccgttggact gtctctgtta ggtcaaatgt gcgctatata    1740

tataccattt ttccaaagta tctttaaaac tgagaaactt ggtatctctg atatactatt    1800

gttattgctc atcagcagta gcgttttcat cgttgatgaa ttgagaaaat tgtggacgag    1860

gaaaaagaat gaagaagact caacgtattt ctcaaatgtt tga                      1903


<210> 136
<211> 600
<212> PRT
<213> Saccharomyces cerevisiae


<220> 
<223> Sc_PMR1 protein sequence of CEN.PK113-7D

<400> 136
Ala Ile Val Arg Arg Leu Pro Ser Val Glu Thr Leu Gly Ser Val Asn 
1               5                   10                  15      
Val Ile Cys Ser Asp Lys Thr Gly Thr Leu Thr Ser Asn His Met Thr 
            20                  25                  30          
Val Ser Lys Leu Trp Cys Leu Asp Ser Met Ser Asn Lys Leu Asn Val 
        35                  40                  45              
Leu Ser Leu Asp Lys Asn Lys Lys Thr Lys Asn Ser Asn Gly Asn Leu 
    50                  55                  60                  
Lys Asn Tyr Leu Thr Glu Asp Val Arg Glu Thr Leu Thr Ile Gly Asn 
65                  70                  75                  80  
Leu Cys Asn Asn Ala Ser Phe Ser Gln Glu His Ala Ile Phe Leu Gly 
                85                  90                  95      
Asn Pro Thr Asp Val Ala Leu Leu Glu Gln Leu Ala Asn Phe Glu Met 
            100                 105                 110         
Pro Asp Ile Arg Asn Thr Val Gln Lys Val Gln Glu Leu Pro Phe Asn 
        115                 120                 125             
Ser Lys Arg Lys Leu Met Ala Thr Lys Ile Leu Asn Pro Val Asp Asn 
    130                 135                 140                 
Lys Cys Thr Val Tyr Val Lys Gly Ala Phe Glu Arg Ile Leu Glu Tyr 
145                 150                 155                 160 
Ser Thr Ser Tyr Leu Lys Ser Lys Gly Lys Lys Thr Glu Lys Leu Thr 
                165                 170                 175     
Glu Ala Gln Lys Ala Thr Ile Asn Glu Cys Ala Asn Ser Met Ala Ser 
            180                 185                 190         
Glu Gly Leu Arg Val Phe Gly Phe Ala Lys Leu Thr Leu Ser Asp Ser 
        195                 200                 205             
Ser Thr Pro Leu Thr Glu Asp Leu Ile Lys Asp Leu Thr Phe Thr Gly 
    210                 215                 220                 
Leu Ile Gly Met Asn Asp Pro Pro Arg Pro Asn Val Lys Phe Ala Ile 
225                 230                 235                 240 
Glu Gln Leu Leu Gln Gly Gly Val His Ile Ile Met Ile Thr Gly Asp 
                245                 250                 255     
Ser Glu Asn Thr Ala Val Asn Ile Ala Lys Gln Ile Gly Ile Pro Val 
            260                 265                 270         
Ile Asp Pro Lys Leu Ser Val Leu Ser Gly Asp Lys Leu Asp Glu Met 
        275                 280                 285             
Ser Asp Asp Gln Leu Ala Asn Val Ile Asp His Val Asn Ile Phe Ala 
    290                 295                 300                 
Arg Ala Thr Pro Glu His Lys Leu Asn Ile Val Arg Ala Leu Arg Lys 
305                 310                 315                 320 
Arg Gly Asp Val Val Ala Met Thr Gly Asp Gly Val Asn Asp Ala Pro 
                325                 330                 335     
Ala Leu Lys Leu Ser Asp Ile Gly Val Ser Met Gly Arg Ile Gly Thr 
            340                 345                 350         
Asp Val Ala Lys Glu Ala Ser Asp Met Val Leu Thr Asp Asp Asp Phe 
        355                 360                 365             
Ser Thr Ile Leu Thr Ala Ile Glu Glu Gly Lys Gly Ile Phe Asn Asn 
    370                 375                 380                 
Ile Gln Asn Phe Leu Thr Phe Gln Leu Ser Thr Ser Val Ala Ala Leu 
385                 390                 395                 400 
Ser Leu Val Ala Leu Ser Thr Ala Phe Lys Leu Pro Asn Pro Leu Asn 
                405                 410                 415     
Ala Met Gln Ile Leu Trp Ile Asn Ile Leu Met Asp Gly Pro Pro Ala 
            420                 425                 430         
Gln Ser Leu Gly Val Glu Pro Val Asp His Glu Val Met Lys Lys Pro 
        435                 440                 445             
Pro Arg Lys Arg Thr Asp Lys Ile Leu Thr His Asp Val Met Lys Arg 
    450                 455                 460                 
Leu Leu Thr Thr Ala Ala Cys Ile Ile Val Gly Thr Val Tyr Ile Phe 
465                 470                 475                 480 
Val Lys Glu Met Ala Glu Asp Gly Lys Val Thr Ala Arg Asp Thr Thr 
                485                 490                 495     
Met Thr Phe Thr Cys Phe Val Phe Phe Asp Met Phe Asn Ala Leu Ala 
            500                 505                 510         
Cys Arg His Asn Thr Lys Ser Ile Phe Glu Ile Gly Phe Phe Thr Asn 
        515                 520                 525             
Lys Met Phe Asn Tyr Ala Val Gly Leu Ser Leu Leu Gly Gln Met Cys 
    530                 535                 540                 
Ala Ile Tyr Ile Pro Phe Phe Gln Ser Ile Phe Lys Thr Glu Lys Leu 
545                 550                 555                 560 
Gly Ile Ser Asp Ile Leu Leu Leu Leu Leu Ile Ser Ser Ser Val Phe 
                565                 570                 575     
Ile Val Asp Glu Leu Arg Lys Leu Trp Thr Arg Lys Lys Asn Glu Glu 
            580                 585                 590         
Asp Ser Thr Tyr Phe Ser Asn Val 
        595                 600 

<210> 137
<211> 1175
<212> DNA
<213> Saccharomyces cerevisiae


<220> 
<223> Sc_PMR1 WT integration construct

<400> 137
atgcaaattc tttggataaa tattttaatg gatgggccac cagctcaatc cttaggtgtg      60

gaacctgttg atcatgaagt tatgaaaaaa cctccaagaa aacgtaccga taaaattttg     120

acccatgatg taatgaaacg tttactaacc accgcggcct gtatcatcgt tgggacagtt     180

tacatttttg ttaaagagat ggccgaagat ggtaaagtaa ctgctagaga tactactatg     240

acatttactt gttttgtttt ttttgatatg tttaatgctt tggcctgcag acataacaca     300

aagtcaatct tcgaaatcgg ctttttcacg aacaaaatgt tcaactacgc cgttggactg     360

tctctgttag gtcaaatgtg cgctatatat ataccatttt tccaaagtat ctttaaaact     420

gagaaacttg gtatctctga tatactattg ttattgctca tcagcagtag cgttttcatc     480

gttgatgaat tgagaaaatt gtggacgagg aaaaagaatg aagaagactc aacgtatttc     540

tcaaatgttt gatatgtcac attttgtgct tttatcgttt ttccttcctt ccctttatct     600

ttcatgagga cgcccaaccc tattgaggta aatgtactaa ttaatgggaa catatgtaga     660

tgtatatatg tacatatatt tacaacggat actaagataa acatgtatgg gcgacttttc     720

gtatatacaa agcaatataa aattttattc tttccttctt tttccggact agatataaaa     780

tctccgtaat cagtggttat gattttcaaa agttaatcac agttttattt aaaactgtat     840

acaattattt gcagccgcct ttcgcaatct gttcataaca gctaatcctt ctccttcctc     900

atttatacct tcgacaaata ttaggtctac tttatcattt tcatccactt ttcttaaagc     960

tgcaaacaag ttagtctgta tttcttcacc agattggcca agtctttcga tgatgaaagt    1020

tttcgaagaa aaatcgggtt cattaaagat cttagattgt aagtcggaat cacgtaattt    1080

tagggaagtt aaaatcgcaa ttttttttat attactgttg gcttttaatt ccgtttcgat    1140

taacctcttt aatctttcca tcctatcaac gccct                               1175


<210> 138
<211> 2525
<212> DNA
<213> Artificial Sequence


<220> 
<223> Sc_PMR1 Q783A integration construct

<400> 138
atatcggtat ccttagcggt tgctgctatt ccagaagggt taccaattat tgtcactgtt      60

actttggcat tgggtgttct gagaatggcc aagcgtaaag ccatcgtgag aaggttacca     120

agtgtcgaaa ctttaggctc tgtcaacgtt atctgctccg acaaaacagg tacactaacc     180

tcaaaccaca tgaccgtatc taaactttgg tgcttggaca gtatgtccaa taagctaaac     240

gtcctctcat tagacaaaaa taagaagact aaaaattcta atggaaattt gaaaaactat     300

ttgactgaag acgttaggga aactctaact atcggtaatc tctgtaataa tgcatctttc     360

tctcaagaac atgccatatt tctgggaaat cctactgatg tagctctttt agagcaattg     420

gcaaactttg aaatgcctga tatcagaaac accgttcaaa aagttcagga acttccattt     480

aactcgaaaa gaaaattaat ggcaaccaag attctcaacc ctgtcgacaa taagtgtaca     540

gtttatgtta aaggtgcatt tgaaagaatt cttgagtact ccacaagtta tttgaaatca     600

aagggtaaaa aaactgaaaa gttgactgaa gcccaaaaag ctacgataaa tgagtgcgca     660

aattctatgg catctgaagg tttgcgtgtc tttggatttg ctaaactaac tttgtctgat     720

tcatcaactc ctctaaccga agacctaatc aaagatttaa cctttactgg tttaatcggt     780

atgaatgacc caccaagacc gaacgttaaa tttgccatcg aacaattact acaaggtggt     840

gtccatatta ttatgatcac tggtgattct gagaataccg cagtaaacat tgcaaaacaa     900

attggtattc cagttattga tccaaagctt tccgttttat ccggtgataa attagatgaa     960

atgtcagatg atcaactggc caatgtcatc gaccacgtta atatttttgc tcgtgctacg    1020

cctgagcata agttaaacat tgttcgtgca ttaagaaaga ggggtgatgt ggtagcaatg    1080

actggtgatg gtgttaacga cgctcctgcg ttgaaacttt cagatattgg tgtttctatg    1140

ggtagaattg gtacagatgt agccaaagaa gcctcagata tggtcttaac tgatgatgac    1200

ttcagtacta ttttaactgc cattgaagag ggtaaaggta tctttaataa tattcagaat    1260

ttcctgactt ttcaattgtc tacttctgtt gccgcactat cattagttgc actatctaca    1320

gcgtttaaac tacccaatcc actgaacgca atgcaaattc tttggataaa tattttaatg    1380

gatgggccac cagctgcttc cttaggtgtg gaacctgttg atcatgaagt tatgaaaaaa    1440

cctccaagaa aacgtaccga taaaattttg acccatgatg taatgaaacg tttactaacc    1500

accgcggcct gtatcatcgt tgggacagtt tacatttttg ttaaagagat ggccgaagat    1560

ggtaaagtaa ctgctagaga tactactatg acatttactt gttttgtttt ttttgatatg    1620

tttaatgctt tggcctgcag acataacaca aagtcaatct tcgaaatcgg ctttttcacg    1680

aacaaaatgt tcaactacgc cgttggactg tctctgttag gtcaaatgtg cgctatatat    1740

ataccatttt tccaaagtat ctttaaaact gagaaacttg gtatctctga tatactattg    1800

ttattgctca tcagcagtag cgttttcatc gttgatgaat tgagaaaatt gtggacgagg    1860

aaaaagaatg aagaagactc aacgtatttc tcaaatgttt gatatgtcac attttgtgct    1920

tttatcgttt ttccttcctt ccctttatct ttcatgagga cgcccaaccc tattgaggta    1980

aatgtactaa ttaatgggaa catatgtaga tgtatatatg tacatatatt tacaacggat    2040

actaagataa acatgtatgg gcgacttttc gtatatacaa agcaatataa aattttattc    2100

tttccttctt tttccggact agatataaaa tctccgtaat cagtggttat gattttcaaa    2160

agttaatcac agttttattt aaaactgtat acaattattt gcagccgcct ttcgcaatct    2220

gttcataaca gctaatcctt ctccttcctc atttatacct tcgacaaata ttaggtctac    2280

tttatcattt tcatccactt ttcttaaagc tgcaaacaag ttagtctgta tttcttcacc    2340

agattggcca agtctttcga tgatgaaagt tttcgaagaa aaatcgggtt cattaaagat    2400

cttagattgt aagtcggaat cacgtaattt tagggaagtt aaaatcgcaa ttttttttat    2460

attactgttg gcttttaatt ccgtttcgat taacctcttt aatctttcca tcctatcaac    2520

gccct                                                                2525


<210> 139
<211> 1175
<212> DNA
<213> Artificial Sequence


<220> 
<223> Sc_PMR1 Q783V integration construct

<400> 139
atgcaaattc tttggataaa tattttaatg gatgggccac cagctgtttc cttaggtgtg      60

gaacctgttg atcatgaagt tatgaaaaaa cctccaagaa aacgtaccga taaaattttg     120

acccatgatg taatgaaacg tttactaacc accgcggcct gtatcatcgt tgggacagtt     180

tacatttttg ttaaagagat ggccgaagat ggtaaagtaa ctgctagaga tactactatg     240

acatttactt gttttgtttt ttttgatatg tttaatgctt tggcctgcag acataacaca     300

aagtcaatct tcgaaatcgg ctttttcacg aacaaaatgt tcaactacgc cgttggactg     360

tctctgttag gtcaaatgtg cgctatatat ataccatttt tccaaagtat ctttaaaact     420

gagaaacttg gtatctctga tatactattg ttattgctca tcagcagtag cgttttcatc     480

gttgatgaat tgagaaaatt gtggacgagg aaaaagaatg aagaagactc aacgtatttc     540

tcaaatgttt gatatgtcac attttgtgct tttatcgttt ttccttcctt ccctttatct     600

ttcatgagga cgcccaaccc tattgaggta aatgtactaa ttaatgggaa catatgtaga     660

tgtatatatg tacatatatt tacaacggat actaagataa acatgtatgg gcgacttttc     720

gtatatacaa agcaatataa aattttattc tttccttctt tttccggact agatataaaa     780

tctccgtaat cagtggttat gattttcaaa agttaatcac agttttattt aaaactgtat     840

acaattattt gcagccgcct ttcgcaatct gttcataaca gctaatcctt ctccttcctc     900

atttatacct tcgacaaata ttaggtctac tttatcattt tcatccactt ttcttaaagc     960

tgcaaacaag ttagtctgta tttcttcacc agattggcca agtctttcga tgatgaaagt    1020

tttcgaagaa aaatcgggtt cattaaagat cttagattgt aagtcggaat cacgtaattt    1080

tagggaagtt aaaatcgcaa ttttttttat attactgttg gcttttaatt ccgtttcgat    1140

taacctcttt aatctttcca tcctatcaac gccct                               1175


<210> 140
<211> 2525
<212> DNA
<213> Artificial Sequence


<220> 
<223> Sc_PMR1 V335G integration construct

<400> 140
atatcggtat ccttagcggt tgctgctatt ccagaagggt taccaattat tggtactgtt      60

actttggcat tgggtgttct gagaatggcc aagcgtaaag ccatcgtgag aaggttacca     120

agtgtcgaaa ctttaggctc tgtcaacgtt atctgctccg acaaaacagg tacactaacc     180

tcaaaccaca tgaccgtatc taaactttgg tgcttggaca gtatgtccaa taagctaaac     240

gtcctctcat tagacaaaaa taagaagact aaaaattcta atggaaattt gaaaaactat     300

ttgactgaag acgttaggga aactctaact atcggtaatc tctgtaataa tgcatctttc     360

tctcaagaac atgccatatt tctgggaaat cctactgatg tagctctttt agagcaattg     420

gcaaactttg aaatgcctga tatcagaaac accgttcaaa aagttcagga acttccattt     480

aactcgaaaa gaaaattaat ggcaaccaag attctcaacc ctgtcgacaa taagtgtaca     540

gtttatgtta aaggtgcatt tgaaagaatt cttgagtact ccacaagtta tttgaaatca     600

aagggtaaaa aaactgaaaa gttgactgaa gcccaaaaag ctacgataaa tgagtgcgca     660

aattctatgg catctgaagg tttgcgtgtc tttggatttg ctaaactaac tttgtctgat     720

tcatcaactc ctctaaccga agacctaatc aaagatttaa cctttactgg tttaatcggt     780

atgaatgacc caccaagacc gaacgttaaa tttgccatcg aacaattact acaaggtggt     840

gtccatatta ttatgatcac tggtgattct gagaataccg cagtaaacat tgcaaaacaa     900

attggtattc cagttattga tccaaagctt tccgttttat ccggtgataa attagatgaa     960

atgtcagatg atcaactggc caatgtcatc gaccacgtta atatttttgc tcgtgctacg    1020

cctgagcata agttaaacat tgttcgtgca ttaagaaaga ggggtgatgt ggtagcaatg    1080

actggtgatg gtgttaacga cgctcctgcg ttgaaacttt cagatattgg tgtttctatg    1140

ggtagaattg gtacagatgt agccaaagaa gcctcagata tggtcttaac tgatgatgac    1200

ttcagtacta ttttaactgc cattgaagag ggtaaaggta tctttaataa tattcagaat    1260

ttcctgactt ttcaattgtc tacttctgtt gccgcactat cattagttgc actatctaca    1320

gcgtttaaac tacccaatcc actgaacgca atgcaaattc tttggataaa tattttaatg    1380

gatgggccac cagctcaatc cttaggtgtg gaacctgttg atcatgaagt tatgaaaaaa    1440

cctccaagaa aacgtaccga taaaattttg acccatgatg taatgaaacg tttactaacc    1500

accgcggcct gtatcatcgt tgggacagtt tacatttttg ttaaagagat ggccgaagat    1560

ggtaaagtaa ctgctagaga tactactatg acatttactt gttttgtttt ttttgatatg    1620

tttaatgctt tggcctgcag acataacaca aagtcaatct tcgaaatcgg ctttttcacg    1680

aacaaaatgt tcaactacgc cgttggactg tctctgttag gtcaaatgtg cgctatatat    1740

ataccatttt tccaaagtat ctttaaaact gagaaacttg gtatctctga tatactattg    1800

ttattgctca tcagcagtag cgttttcatc gttgatgaat tgagaaaatt gtggacgagg    1860

aaaaagaatg aagaagactc aacgtatttc tcaaatgttt gatatgtcac attttgtgct    1920

tttatcgttt ttccttcctt ccctttatct ttcatgagga cgcccaaccc tattgaggta    1980

aatgtactaa ttaatgggaa catatgtaga tgtatatatg tacatatatt tacaacggat    2040

actaagataa acatgtatgg gcgacttttc gtatatacaa agcaatataa aattttattc    2100

tttccttctt tttccggact agatataaaa tctccgtaat cagtggttat gattttcaaa    2160

agttaatcac agttttattt aaaactgtat acaattattt gcagccgcct ttcgcaatct    2220

gttcataaca gctaatcctt ctccttcctc atttatacct tcgacaaata ttaggtctac    2280

tttatcattt tcatccactt ttcttaaagc tgcaaacaag ttagtctgta tttcttcacc    2340

agattggcca agtctttcga tgatgaaagt tttcgaagaa aaatcgggtt cattaaagat    2400

cttagattgt aagtcggaat cacgtaattt tagggaagtt aaaatcgcaa ttttttttat    2460

attactgttg gcttttaatt ccgtttcgat taacctcttt aatctttcca tcctatcaac    2520

gccct                                                                2525


<210> 141
<211> 1175
<212> DNA
<213> Artificial Sequence


<220> 
<223> Sc_PMR1 V335A integration construct

<400> 141
atgcaaattc tttggataaa tattttaatg gatgggccac cagctcaatc cttaggtgtg      60

gaacctgttg atcatgaagt tatgaaaaaa cctccaagaa aacgtaccga taaaattttg     120

acccatgatg taatgaaacg tttactaacc accgcggcct gtatcatcgt tgggacagtt     180

tacatttttg ttaaagagat ggccgaagat ggtaaagtaa ctgctagaga tactactatg     240

acatttactt gttttgtttt ttttgatatg tttaatgctt tggcctgcag acataacaca     300

aagtcaatct tcgaaatcgg ctttttcacg aacaaaatgt tcaactacgc cgttggactg     360

tctctgttag gtcaaatgtg cgctatatat ataccatttt tccaaagtat ctttaaaact     420

gagaaacttg gtatctctga tatactattg ttattgctca tcagcagtag cgttttcatc     480

gttgatgaat tgagaaaatt gtggacgagg aaaaagaatg aagaagactc aacgtatttc     540

tcaaatgttt gatatgtcac attttgtgct tttatcgttt ttccttcctt ccctttatct     600

ttcatgagga cgcccaaccc tattgaggta aatgtactaa ttaatgggaa catatgtaga     660

tgtatatatg tacatatatt tacaacggat actaagataa acatgtatgg gcgacttttc     720

gtatatacaa agcaatataa aattttattc tttccttctt tttccggact agatataaaa     780

tctccgtaat cagtggttat gattttcaaa agttaatcac agttttattt aaaactgtat     840

acaattattt gcagccgcct ttcgcaatct gttcataaca gctaatcctt ctccttcctc     900

atttatacct tcgacaaata ttaggtctac tttatcattt tcatccactt ttcttaaagc     960

tgcaaacaag ttagtctgta tttcttcacc agattggcca agtctttcga tgatgaaagt    1020

tttcgaagaa aaatcgggtt cattaaagat cttagattgt aagtcggaat cacgtaattt    1080

tagggaagtt aaaatcgcaa ttttttttat attactgttg gcttttaatt ccgtttcgat    1140

taacctcttt aatctttcca tcctatcaac gccct                               1175


<210> 142
<211> 1175
<212> DNA
<213> Artificial Sequence


<220> 
<223> Sc_PMR1 V335Q integration construct

<400> 142
atgcaaattc tttggataaa tattttaatg gatgggccac cagctcaatc cttaggtgtg      60

gaacctgttg atcatgaagt tatgaaaaaa cctccaagaa aacgtaccga taaaattttg     120

acccatgatg taatgaaacg tttactaacc accgcggcct gtatcatcgt tgggacagtt     180

tacatttttg ttaaagagat ggccgaagat ggtaaagtaa ctgctagaga tactactatg     240

acatttactt gttttgtttt ttttgatatg tttaatgctt tggcctgcag acataacaca     300

aagtcaatct tcgaaatcgg ctttttcacg aacaaaatgt tcaactacgc cgttggactg     360

tctctgttag gtcaaatgtg cgctatatat ataccatttt tccaaagtat ctttaaaact     420

gagaaacttg gtatctctga tatactattg ttattgctca tcagcagtag cgttttcatc     480

gttgatgaat tgagaaaatt gtggacgagg aaaaagaatg aagaagactc aacgtatttc     540

tcaaatgttt gatatgtcac attttgtgct tttatcgttt ttccttcctt ccctttatct     600

ttcatgagga cgcccaaccc tattgaggta aatgtactaa ttaatgggaa catatgtaga     660

tgtatatatg tacatatatt tacaacggat actaagataa acatgtatgg gcgacttttc     720

gtatatacaa agcaatataa aattttattc tttccttctt tttccggact agatataaaa     780

tctccgtaat cagtggttat gattttcaaa agttaatcac agttttattt aaaactgtat     840

acaattattt gcagccgcct ttcgcaatct gttcataaca gctaatcctt ctccttcctc     900

atttatacct tcgacaaata ttaggtctac tttatcattt tcatccactt ttcttaaagc     960

tgcaaacaag ttagtctgta tttcttcacc agattggcca agtctttcga tgatgaaagt    1020

tttcgaagaa aaatcgggtt cattaaagat cttagattgt aagtcggaat cacgtaattt    1080

tagggaagtt aaaatcgcaa ttttttttat attactgttg gcttttaatt ccgtttcgat    1140

taacctcttt aatctttcca tcctatcaac gccct                               1175


<210> 143
<211> 1175
<212> DNA
<213> Artificial Sequence


<220> 
<223> Sc_PMR1 Q783V/V335Q integration construct

<400> 143
atgcaaattc tttggataaa tattttaatg gatgggccac cagctgtttc cttaggtgtg      60

gaacctgttg atcatgaagt tatgaaaaaa cctccaagaa aacgtaccga taaaattttg     120

acccatgatg taatgaaacg tttactaacc accgcggcct gtatcatcgt tgggacagtt     180

tacatttttg ttaaagagat ggccgaagat ggtaaagtaa ctgctagaga tactactatg     240

acatttactt gttttgtttt ttttgatatg tttaatgctt tggcctgcag acataacaca     300

aagtcaatct tcgaaatcgg ctttttcacg aacaaaatgt tcaactacgc cgttggactg     360

tctctgttag gtcaaatgtg cgctatatat ataccatttt tccaaagtat ctttaaaact     420

gagaaacttg gtatctctga tatactattg ttattgctca tcagcagtag cgttttcatc     480

gttgatgaat tgagaaaatt gtggacgagg aaaaagaatg aagaagactc aacgtatttc     540

tcaaatgttt gatatgtcac attttgtgct tttatcgttt ttccttcctt ccctttatct     600

ttcatgagga cgcccaaccc tattgaggta aatgtactaa ttaatgggaa catatgtaga     660

tgtatatatg tacatatatt tacaacggat actaagataa acatgtatgg gcgacttttc     720

gtatatacaa agcaatataa aattttattc tttccttctt tttccggact agatataaaa     780

tctccgtaat cagtggttat gattttcaaa agttaatcac agttttattt aaaactgtat     840

acaattattt gcagccgcct ttcgcaatct gttcataaca gctaatcctt ctccttcctc     900

atttatacct tcgacaaata ttaggtctac tttatcattt tcatccactt ttcttaaagc     960

tgcaaacaag ttagtctgta tttcttcacc agattggcca agtctttcga tgatgaaagt    1020

tttcgaagaa aaatcgggtt cattaaagat cttagattgt aagtcggaat cacgtaattt    1080

tagggaagtt aaaatcgcaa ttttttttat attactgttg gcttttaatt ccgtttcgat    1140

taacctcttt aatctttcca tcctatcaac gccct                               1175


<210> 144
<211> 1175
<212> DNA
<213> Artificial Sequence


<220> 
<223> Sc_PMR1 Q783L/V335A integration construct

<400> 144
atgcaaattc tttggataaa tattttaatg gatgggccac cagctttgtc cttaggtgtg      60

gaacctgttg atcatgaagt tatgaaaaaa cctccaagaa aacgtaccga taaaattttg     120

acccatgatg taatgaaacg tttactaacc accgcggcct gtatcatcgt tgggacagtt     180

tacatttttg ttaaagagat ggccgaagat ggtaaagtaa ctgctagaga tactactatg     240

acatttactt gttttgtttt ttttgatatg tttaatgctt tggcctgcag acataacaca     300

aagtcaatct tcgaaatcgg ctttttcacg aacaaaatgt tcaactacgc cgttggactg     360

tctctgttag gtcaaatgtg cgctatatat ataccatttt tccaaagtat ctttaaaact     420

gagaaacttg gtatctctga tatactattg ttattgctca tcagcagtag cgttttcatc     480

gttgatgaat tgagaaaatt gtggacgagg aaaaagaatg aagaagactc aacgtatttc     540

tcaaatgttt gatatgtcac attttgtgct tttatcgttt ttccttcctt ccctttatct     600

ttcatgagga cgcccaaccc tattgaggta aatgtactaa ttaatgggaa catatgtaga     660

tgtatatatg tacatatatt tacaacggat actaagataa acatgtatgg gcgacttttc     720

gtatatacaa agcaatataa aattttattc tttccttctt tttccggact agatataaaa     780

tctccgtaat cagtggttat gattttcaaa agttaatcac agttttattt aaaactgtat     840

acaattattt gcagccgcct ttcgcaatct gttcataaca gctaatcctt ctccttcctc     900

atttatacct tcgacaaata ttaggtctac tttatcattt tcatccactt ttcttaaagc     960

tgcaaacaag ttagtctgta tttcttcacc agattggcca agtctttcga tgatgaaagt    1020

tttcgaagaa aaatcgggtt cattaaagat cttagattgt aagtcggaat cacgtaattt    1080

tagggaagtt aaaatcgcaa ttttttttat attactgttg gcttttaatt ccgtttcgat    1140

taacctcttt aatctttcca tcctatcaac gccct                               1175


<210> 145
<211> 2525
<212> DNA
<213> Artificial Sequence


<220> 
<223> Sc_PMR1 Q783L/V335T integration construct

<400> 145
atatcggtat ccttagcggt tgctgctatt ccagaagggt taccaattat tactactgtt      60

actttggcat tgggtgttct gagaatggcc aagcgtaaag ccatcgtgag aaggttacca     120

agtgtcgaaa ctttaggctc tgtcaacgtt atctgctccg acaaaacagg tacactaacc     180

tcaaaccaca tgaccgtatc taaactttgg tgcttggaca gtatgtccaa taagctaaac     240

gtcctctcat tagacaaaaa taagaagact aaaaattcta atggaaattt gaaaaactat     300

ttgactgaag acgttaggga aactctaact atcggtaatc tctgtaataa tgcatctttc     360

tctcaagaac atgccatatt tctgggaaat cctactgatg tagctctttt agagcaattg     420

gcaaactttg aaatgcctga tatcagaaac accgttcaaa aagttcagga acttccattt     480

aactcgaaaa gaaaattaat ggcaaccaag attctcaacc ctgtcgacaa taagtgtaca     540

gtttatgtta aaggtgcatt tgaaagaatt cttgagtact ccacaagtta tttgaaatca     600

aagggtaaaa aaactgaaaa gttgactgaa gcccaaaaag ctacgataaa tgagtgcgca     660

aattctatgg catctgaagg tttgcgtgtc tttggatttg ctaaactaac tttgtctgat     720

tcatcaactc ctctaaccga agacctaatc aaagatttaa cctttactgg tttaatcggt     780

atgaatgacc caccaagacc gaacgttaaa tttgccatcg aacaattact acaaggtggt     840

gtccatatta ttatgatcac tggtgattct gagaataccg cagtaaacat tgcaaaacaa     900

attggtattc cagttattga tccaaagctt tccgttttat ccggtgataa attagatgaa     960

atgtcagatg atcaactggc caatgtcatc gaccacgtta atatttttgc tcgtgctacg    1020

cctgagcata agttaaacat tgttcgtgca ttaagaaaga ggggtgatgt ggtagcaatg    1080

actggtgatg gtgttaacga cgctcctgcg ttgaaacttt cagatattgg tgtttctatg    1140

ggtagaattg gtacagatgt agccaaagaa gcctcagata tggtcttaac tgatgatgac    1200

ttcagtacta ttttaactgc cattgaagag ggtaaaggta tctttaataa tattcagaat    1260

ttcctgactt ttcaattgtc tacttctgtt gccgcactat cattagttgc actatctaca    1320

gcgtttaaac tacccaatcc actgaacgca atgcaaattc tttggataaa tattttaatg    1380

gatgggccac cagctttgtc cttaggtgtg gaacctgttg atcatgaagt tatgaaaaaa    1440

cctccaagaa aacgtaccga taaaattttg acccatgatg taatgaaacg tttactaacc    1500

accgcggcct gtatcatcgt tgggacagtt tacatttttg ttaaagagat ggccgaagat    1560

ggtaaagtaa ctgctagaga tactactatg acatttactt gttttgtttt ttttgatatg    1620

tttaatgctt tggcctgcag acataacaca aagtcaatct tcgaaatcgg ctttttcacg    1680

aacaaaatgt tcaactacgc cgttggactg tctctgttag gtcaaatgtg cgctatatat    1740

ataccatttt tccaaagtat ctttaaaact gagaaacttg gtatctctga tatactattg    1800

ttattgctca tcagcagtag cgttttcatc gttgatgaat tgagaaaatt gtggacgagg    1860

aaaaagaatg aagaagactc aacgtatttc tcaaatgttt gatatgtcac attttgtgct    1920

tttatcgttt ttccttcctt ccctttatct ttcatgagga cgcccaaccc tattgaggta    1980

aatgtactaa ttaatgggaa catatgtaga tgtatatatg tacatatatt tacaacggat    2040

actaagataa acatgtatgg gcgacttttc gtatatacaa agcaatataa aattttattc    2100

tttccttctt tttccggact agatataaaa tctccgtaat cagtggttat gattttcaaa    2160

agttaatcac agttttattt aaaactgtat acaattattt gcagccgcct ttcgcaatct    2220

gttcataaca gctaatcctt ctccttcctc atttatacct tcgacaaata ttaggtctac    2280

tttatcattt tcatccactt ttcttaaagc tgcaaacaag ttagtctgta tttcttcacc    2340

agattggcca agtctttcga tgatgaaagt tttcgaagaa aaatcgggtt cattaaagat    2400

cttagattgt aagtcggaat cacgtaattt tagggaagtt aaaatcgcaa ttttttttat    2460

attactgttg gcttttaatt ccgtttcgat taacctcttt aatctttcca tcctatcaac    2520

gccct                                                                2525


<210> 146
<211> 4075
<212> DNA
<213> Artificial Sequence


<220> 
<223> Sc_PMR1 Q783E V335A integration construct

<400> 146
ccatggctac tgctatttcg tccacagctt aatacttacc gaaccaggcc ttgcggctct       60

taccctgcgt gcccgcgcac cctggcgact tcgcctcgtt ttggcgattt tcttagcaaa      120

tattctttca cgatcgtagt ggtggaaggt gggtgaaaag agttgcgttc ctagcaagga      180

tcggttggta tcaattgcac gttcagctca tccctagata accacttaac agcaaagccg      240

atcgttaaac ttttgtattc tacattctag ttgcattttt tttttttttc attttgttac      300

atcaagacaa gattctctat ttaaagaagt actttttttg ttaacagtgt ggaccctacc      360

tatcgttata tcgagatctt gttccctagg ccatcgtaca ctatagccct tccttcaagc      420

acttaataga aaaacctctt tttctacacc atcataatag tgtttgctcg ccccgttctt      480

tccattccca tttataacaa tactagtaac tataataata tccttacgac tgggcaagac      540

aagacgaagc aaggccagca cagacgtaag cttaagtgta agtaaaagat aagataatta      600

tgagtgacaa tccatttaat gcgagtcttc ttgacgagga ctcaaaccgt gagagagaaa      660

tactagatgc cacagcagag gccctttcga aaccaagccc ttctttagag tattgtactt      720

tatccgtgga cgaagctcta gaaaaactgg acactgacaa aaacggtggt ttacgatcat      780

ctaacgaggc caacaatagg agatcacttt atggccccaa tgaaataacc gtagaagatg      840

atgaaagtct tttcaagaag ttcttgtcaa atttcattga ggatcgaatg attctacttt      900

taataggatc cgcagtggtc tctcttttta tgggtaacat tgatgatgct gttagtatca      960

cactggccat tttcatagtt gtcactgtcg gttttgtcca agaatatagg tctgaaaaat     1020

ctctagaagc gttgaataaa ttggttcctg ctgaatgtca cttaatgaga tgtggtcaag     1080

agagtcatgt actggcttcc accttggttc ctggtgattt agtgcacttc agaataggtg     1140

acagaatccc cgcagacatt agaattattg aagcaatcga tttatccatc gatgaaagta     1200

atttaactgg tgaaaatgaa ccggtacata aaacctcaca aacgatcgaa aaatcttcct     1260

ttaacgatca gcctaattca attgtaccga tttctgagag atcttgtata gcttatatgg     1320

gtacattagt caaggaaggt catggtaagg gtatcgtcgt aggaacaggt acaaacacat     1380

cctttggtgc cgtttttgaa atgatgaata atattgaaaa accgaagact ccattgcagt     1440

taacaatgga caaattggga aaggacttgt cactggttag cttcatagtt attggtatga     1500

tttgtttagt tggtatcata caaggtagat cttggttaga aatgttccaa atatcggtat     1560

ccttagcggt tgctgctatt ccagaagggt taccaattat tgctactgtt actttggcat     1620

tgggtgttct gagaatggcc aagcgtaaag ccatcgtgag aaggttacca agtgtcgaaa     1680

ctttaggctc tgtcaacgtt atctgctccg acaaaacagg tacactaacc tcaaaccaca     1740

tgaccgtatc taaactttgg tgcttggaca gtatgtccaa taagctaaac gtcctctcat     1800

tagacaaaaa taagaagact aaaaattcta atggaaattt gaaaaactat ttgactgaag     1860

acgttaggga aactctaact atcggtaatc tctgtaataa tgcatctttc tctcaagaac     1920

atgccatatt tctgggaaat cctactgatg tagctctttt agagcaattg gcaaactttg     1980

aaatgcctga tatcagaaac accgttcaaa aagttcagga acttccattt aactcgaaaa     2040

gaaaattaat ggcaaccaag attctcaacc ctgtcgacaa taagtgtaca gtttatgtta     2100

aaggtgcatt tgaaagaatt cttgagtact ccacaagtta tttgaaatca aagggtaaaa     2160

aaactgaaaa gttgactgaa gcccaaaaag ctacgataaa tgagtgcgca aattctatgg     2220

catctgaagg tttgcgtgtc tttggatttg ctaaactaac tttgtctgat tcatcaactc     2280

ctctaaccga agacctaatc aaagatttaa cctttactgg tttaatcggt atgaatgacc     2340

caccaagacc gaacgttaaa tttgccatcg aacaattact acaaggtggt gtccatatta     2400

ttatgatcac tggtgattct gagaataccg cagtaaacat tgcaaaacaa attggtattc     2460

cagttattga tccaaagctt tccgttttat ccggtgataa attagatgaa atgtcagatg     2520

atcaactggc caatgtcatc gaccacgtta atatttttgc tcgtgctacg cctgagcata     2580

agttaaacat tgttcgtgca ttaagaaaga ggggtgatgt ggtagcaatg actggtgatg     2640

gtgttaacga cgctcctgcg ttgaaacttt cagatattgg tgtttctatg ggtagaattg     2700

gtacagatgt agccaaagaa gcctcagata tggtcttaac tgatgatgac ttcagtacta     2760

ttttaactgc cattgaagag ggtaaaggta tctttaataa tattcagaat ttcctgactt     2820

ttcaattgtc tacttctgtt gccgcactat cattagttgc actatctaca gcgtttaaac     2880

tacccaatcc actgaacgca atgcaaattc tttggataaa tattttaatg gatgggccac     2940

cagctgaatc cttaggtgtg gaacctgttg atcatgaagt tatgaaaaaa cctccaagaa     3000

aacgtaccga taaaattttg acccatgatg taatgaaacg tttactaacc accgcggcct     3060

gtatcatcgt tgggacagtt tacatttttg ttaaagagat ggccgaagat ggtaaagtaa     3120

ctgctagaga tactactatg acatttactt gttttgtttt ttttgatatg tttaatgctt     3180

tggcctgcag acataacaca aagtcaatct tcgaaatcgg ctttttcacg aacaaaatgt     3240

tcaactacgc cgttggactg tctctgttag gtcaaatgtg cgctatatat ataccatttt     3300

tccaaagtat ctttaaaact gagaaacttg gtatctctga tatactattg ttattgctca     3360

tcagcagtag cgttttcatc gttgatgaat tgagaaaatt gtggacgagg aaaaagaatg     3420

aagaagactc aacgtatttc tcaaatgttt gatatgtcac attttgtgct tttatcgttt     3480

ttccttcctt ccctttatct ttcatgagga cgcccaaccc tattgaggta aatgtactaa     3540

ttaatgggaa catatgtaga tgtatatatg tacatatatt tacaacggat actaagataa     3600

acatgtatgg gcgacttttc gtatatacaa agcaatataa aattttattc tttccttctt     3660

tttccggact agatataaaa tctccgtaat cagtggttat gattttcaaa agttaatcac     3720

agttttattt aaaactgtat acaattattt gcagccgcct ttcgcaatct gttcataaca     3780

gctaatcctt ctccttcctc atttatacct tcgacaaata ttaggtctac tttatcattt     3840

tcatccactt ttcttaaagc tgcaaacaag ttagtctgta tttcttcacc agattggcca     3900

agtctttcga tgatgaaagt tttcgaagaa aaatcgggtt cattaaagat cttagattgt     3960

aagtcggaat cacgtaattt tagggaagtt aaaatcgcaa ttttttttat attactgttg     4020

gcttttaatt ccgtttcgat taacctcttt aatctttcca tcctatcaac gccct          4075


<210> 147
<211> 103
<212> DNA
<213> Artificial Sequence


<220> 
<223> gRNA construction Pho85 deletion

<400> 147
tgcgcatgtt tcggcgttcg aaacttctcc gcagtgaaag ataaatgatc tttgggtaac     60

tttgttacac gttttagagc tagaaatagc aagttaaaat aag                      103


<210> 148
<211> 103
<212> DNA
<213> Artificial Sequence


<220> 
<223> gRNA construction Pho80 deletion

<400> 148
tgcgcatgtt tcggcgttcg aaacttctcc gcagtgaaag ataaatgatc ctcactatat     60

caatatttga gttttagagc tagaaatagc aagttaaaat aag                      103


<210> 149
<211> 103
<212> DNA
<213> Artificial Sequence


<220> 
<223> gRNA construction Bsd2 deletion

<400> 149
tgcgcatgtt tcggcgttcg aaacttctcc gcagtgaaag ataaatgatc gaaatatagc     60

aaatttatta gttttagagc tagaaatagc aagttaaaat aag                      103


<210> 150
<211> 103
<212> DNA
<213> Artificial Sequence


<220> 
<223> gRNA construction Csr1 deletion

<400> 150
tgcgcatgtt tcggcgttcg aaacttctcc gcagtgaaag ataaatgatc agaaccattg     60

tagtcataaa gttttagagc tagaaatagc aagttaaaat aag                      103


<210> 151
<211> 103
<212> DNA
<213> Artificial Sequence


<220> 
<223> gRNA construction Ecm2 deletion

<400> 151
tgcgcatgtt tcggcgttcg aaacttctcc gcagtgaaag ataaatgatc taaaccaatc     60

tatattaata gttttagagc tagaaatagc aagttaaaat aag                      103


<210> 152
<211> 120
<212> DNA
<213> Artificial Sequence


<220> 
<223> PHO85_repair oligo fw

<400> 152
cgtataattg cagataaggg atatatagcg cggcaaactg ggcaaacttg agcaatacca     60

cgcagcccgt cagcgaaaaa ccgtagccat gtatatataa tgataataat catctataat    120


<210> 153
<211> 120
<212> DNA
<213> Artificial Sequence


<220> 
<223> PHO85_repair oligo rv

<400> 153
attatagatg attattatca ttatatatac atggctacgg tttttcgctg acgggctgcg     60

tggtattgct caagtttgcc cagtttgccg cgctatatat cccttatctg caattatacg    120


<210> 154
<211> 120
<212> DNA
<213> Artificial Sequence


<220> 
<223> PHO80_repair oligo fw

<400> 154
caaatttaag tctgcaagct atcataagac gaggatatcc tttggagact catagaaatc     60

tgatttttcg tggggtatta tgaaagcaat catgattgag caaaattaaa actttccatt    120


<210> 155
<211> 120
<212> DNA
<213> Artificial Sequence


<220> 
<223> PHO80_repair oligo rv

<400> 155
aatggaaagt tttaattttg ctcaatcatg attgctttca taatacccca cgaaaaatca     60

gatttctatg agtctccaaa ggatatcctc gtcttatgat agcttgcaga cttaaatttg    120


<210> 156
<211> 120
<212> DNA
<213> Artificial Sequence


<220> 
<223> BSD2_repair oligo fw

<400> 156
ttagatacat aaaaagagca tgagaataac aagaacacgt agtctaggaa actaagcgct     60

gccatcttct tcattatgat aaaaagagat atataacttt tttaagtgat cggctggtaa    120


<210> 157
<211> 120
<212> DNA
<213> Artificial Sequence


<220> 
<223> BSD2_repair oligo rv

<400> 157
ttaccagccg atcacttaaa aaagttatat atctcttttt atcataatga agaagatggc     60

agcgcttagt ttcctagact acgtgttctt gttattctca tgctcttttt atgtatctaa    120


<210> 158
<211> 120
<212> DNA
<213> Artificial Sequence


<220> 
<223> CSR1_repair oligo fw

<400> 158
tacgcacaca gaagcacaaa agcacaaaag cacataagaa gaccacatat aagctagatt     60

gccaagccac gcgacgctcc atactgtata gacctttatt ataaaaacat ctatttcttt    120


<210> 159
<211> 120
<212> DNA
<213> Artificial Sequence


<220> 
<223> CSR1_repair oligo rv

<400> 159
aaagaaatag atgtttttat aataaaggtc tatacagtat ggagcgtcgc gtggcttggc     60

aatctagctt atatgtggtc ttcttatgtg cttttgtgct tttgtgcttc tgtgtgcgta    120


<210> 160
<211> 120
<212> DNA
<213> Artificial Sequence


<220> 
<223> ECM21_repair oligo fw

<400> 160
gaaaggtgac tcttttaaat agagaagaac aagcaagatt tttccctacc cctattgggc     60

attttttcgt tttttatttt tttttgaatt attctacgat aattatatat atagagccac    120


<210> 161
<211> 120
<212> DNA
<213> Artificial Sequence


<220> 
<223> ECM21_repair oligo rv

<400> 161
gtggctctat atatataatt atcgtagaat aattcaaaaa aaaataaaaa acgaaaaaat     60

gcccaatagg ggtagggaaa aatcttgctt gttcttctct atttaaaaga gtcacctttc    120


<210> 162
<211> 20
<212> DNA
<213> Artificial Sequence


<220> 
<223> PHO85_dg fw

<400> 162
ccatagtccg tccagacacg                                                20


<210> 163
<211> 20
<212> DNA
<213> Artificial Sequence


<220> 
<223> PHO85_dg rv

<400> 163
gaacgccaac ggaaagaacc                                                20


<210> 164
<211> 20
<212> DNA
<213> Artificial Sequence


<220> 
<223> PHO80_dg fw

<400> 164
gaatgaggcg aacgtgcttg                                                20


<210> 165
<211> 21
<212> DNA
<213> Artificial Sequence


<220> 
<223> PHO80_dg rv

<400> 165
tctcgaagtg ttctctgtcg c                                              21


<210> 166
<211> 20
<212> DNA
<213> Artificial Sequence


<220> 
<223> BSD2_dg fw

<400> 166
cagccagtaa tcccaggtgg                                                20


<210> 167
<211> 20
<212> DNA
<213> Artificial Sequence


<220> 
<223> BSD2_dg rv

<400> 167
agtttccatg agacaggccg                                                20


<210> 168
<211> 20
<212> DNA
<213> Artificial Sequence


<220> 
<223> CSR1_dg fw

<400> 168
cgtgtttgtt gagctcctgc                                                20


<210> 169
<211> 20
<212> DNA
<213> Artificial Sequence


<220> 
<223> CSR1_dg rv

<400> 169
aacgcagatt gtaggaccgg                                                20


<210> 170
<211> 20
<212> DNA
<213> Artificial Sequence


<220> 
<223> ECM21_dg fw

<400> 170
aagcacagac tccacggaac                                                20


<210> 171
<211> 20
<212> DNA
<213> Artificial Sequence


<220> 
<223> ECM21_dg rv

<400> 171
gctggtgtca attcggcaag                                                20


