                         SEQUENCE LISTING

<110>  University of Copenhagen
 
<120>  Method for processing lignocellulosic biomass

<130>  P4351PC00

<160>  9     

<170>  PatentIn version 3.5

<210>  1
<211>  909
<212>  DNA
<213>  A. nidulans

<400>  1
gtgaagctcc agtacctgct gtccatcctg ctctacgcgt actcctgcac ggccctcatg       60

ctggaccgcc gcgacccgac gcccgggcag ctctcccagg tgaccgactt tggggataac      120

ccgaccaacg tcgggtttta catttacgtg ccccagaacc tggcctcgaa cccggccatc      180

attgtggcta ttcattactg cacgggcacg gcccaggcgt actactccgg gaccccctac      240

gcgcagtacg ctgagacgta cgggtttatc gtcatctacc ccgagtcccc ctactcgggg      300

acgtgctggg acgtctccag ccagagcacg ctcacgcaca acgggggggg taactcgaac      360

tcgattgcga acatggtcga ctggacgatt aaccagtaca acgcggacgc cagccgggtc      420

tacgtcacgg gcacgtcgag cggcgcgatg atgaccaacg tcatggctgc tacctacccc      480

aacctgtttg ccgcggggat cgcgtacgcg ggggtgcccg ctgggtgctt ctacagcgag      540

gcgaacgtcg aggaccagtg gaacagcacc tgcgctcagg ggcagagcat ctccacgcct      600

gagcattggg cccagatcgc tcaggctatg tactccggct acgagggctc ccgccccaag      660

atgcagatct accacgggtc cgcggacgcg acgctctacc cccagaacta ctacgagacg      720

tgcaagcagt gggctggcgt cttcggctac aactacgact cgccccagga ggtccagaac      780

gatacgcctg tggctggctg ggccaagacc atttggggtg agaacctcca gggtatcctg      840

gccgacggcg tcgggcacaa catccagatc cagggtgagg aggatctgaa gtggttcggt      900

tttacgagc                                                              909


<210>  2
<211>  303
<212>  PRT
<213>  A. nidulans

<400>  2

Val Lys Leu Gln Tyr Leu Leu Ser Ile Leu Leu Tyr Ala Tyr Ser Cys 
1               5                   10                  15      


Thr Ala Leu Met Leu Asp Arg Arg Asp Pro Thr Pro Gly Gln Leu Ser 
            20                  25                  30          


Gln Val Thr Asp Phe Gly Asp Asn Pro Thr Asn Val Gly Phe Tyr Ile 
        35                  40                  45              


Tyr Val Pro Gln Asn Leu Ala Ser Asn Pro Ala Ile Ile Val Ala Ile 
    50                  55                  60                  


His Tyr Cys Thr Gly Thr Ala Gln Ala Tyr Tyr Ser Gly Thr Pro Tyr 
65                  70                  75                  80  


Ala Gln Tyr Ala Glu Thr Tyr Gly Phe Ile Val Ile Tyr Pro Glu Ser 
                85                  90                  95      


Pro Tyr Ser Gly Thr Cys Trp Asp Val Ser Ser Gln Ser Thr Leu Thr 
            100                 105                 110         


His Asn Gly Gly Gly Asn Ser Asn Ser Ile Ala Asn Met Val Asp Trp 
        115                 120                 125             


Thr Ile Asn Gln Tyr Asn Ala Asp Ala Ser Arg Val Tyr Val Thr Gly 
    130                 135                 140                 


Thr Ser Ser Gly Ala Met Met Thr Asn Val Met Ala Ala Thr Tyr Pro 
145                 150                 155                 160 


Asn Leu Phe Ala Ala Gly Ile Ala Tyr Ala Gly Val Pro Ala Gly Cys 
                165                 170                 175     


Phe Tyr Ser Glu Ala Asn Val Glu Asp Gln Trp Asn Ser Thr Cys Ala 
            180                 185                 190         


Gln Gly Gln Ser Ile Ser Thr Pro Glu His Trp Ala Gln Ile Ala Gln 
        195                 200                 205             


Ala Met Tyr Ser Gly Tyr Glu Gly Ser Arg Pro Lys Met Gln Ile Tyr 
    210                 215                 220                 


His Gly Ser Ala Asp Ala Thr Leu Tyr Pro Gln Asn Tyr Tyr Glu Thr 
225                 230                 235                 240 


Cys Lys Gln Trp Ala Gly Val Phe Gly Tyr Asn Tyr Asp Ser Pro Gln 
                245                 250                 255     


Glu Val Gln Asn Asp Thr Pro Val Ala Gly Trp Ala Lys Thr Ile Trp 
            260                 265                 270         


Gly Glu Asn Leu Gln Gly Ile Leu Ala Asp Gly Val Gly His Asn Ile 
        275                 280                 285             


Gln Ile Gln Gly Glu Glu Asp Leu Lys Trp Phe Gly Phe Thr Ser 
    290                 295                 300             


<210>  3
<211>  936
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Nucleotide sequence encoding tagged acetylxylan esterase

<400>  3
gtgaagctcc agtacctgct gtccatcctg ctctacgcgt actcctgcac ggccctcatg       60

ctggaccgcc gcgacccgac gcccgggcag ctctcccagg tgaccgactt tggggataac      120

ccgaccaacg tcgggtttta catttacgtg ccccagaacc tggcctcgaa cccggccatc      180

attgtggcta ttcattactg cacgggcacg gcccaggcgt actactccgg gaccccctac      240

gcgcagtacg ctgagacgta cgggtttatc gtcatctacc ccgagtcccc ctactcgggg      300

acgtgctggg acgtctccag ccagagcacg ctcacgcaca acgggggggg taactcgaac      360

tcgattgcga acatggtcga ctggacgatt aaccagtaca acgcggacgc cagccgggtc      420

tacgtcacgg gcacgtcgag cggcgcgatg atgaccaacg tcatggctgc tacctacccc      480

aacctgtttg ccgcggggat cgcgtacgcg ggggtgcccg ctgggtgctt ctacagcgag      540

gcgaacgtcg aggaccagtg gaacagcacc tgcgctcagg ggcagagcat ctccacgcct      600

gagcattggg cccagatcgc tcaggctatg tactccggct acgagggctc ccgccccaag      660

atgcagatct accacgggtc cgcggacgcg acgctctacc cccagaacta ctacgagacg      720

tgcaagcagt gggctggcgt cttcggctac aactacgact cgccccagga ggtccagaac      780

gatacgcctg tggctggctg ggccaagacc atttggggtg agaacctcca gggtatcctg      840

gccgacggcg tcgggcacaa catccagatc cagggtgagg aggatctgaa gtggttcggt      900

tttacgagct ggagccaccc gcagttcgag aagtaa                                936


<210>  4
<211>  311
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Tagged acetylxylan esterase

<400>  4

Val Lys Leu Gln Tyr Leu Leu Ser Ile Leu Leu Tyr Ala Tyr Ser Cys 
1               5                   10                  15      


Thr Ala Leu Met Leu Asp Arg Arg Asp Pro Thr Pro Gly Gln Leu Ser 
            20                  25                  30          


Gln Val Thr Asp Phe Gly Asp Asn Pro Thr Asn Val Gly Phe Tyr Ile 
        35                  40                  45              


Tyr Val Pro Gln Asn Leu Ala Ser Asn Pro Ala Ile Ile Val Ala Ile 
    50                  55                  60                  


His Tyr Cys Thr Gly Thr Ala Gln Ala Tyr Tyr Ser Gly Thr Pro Tyr 
65                  70                  75                  80  


Ala Gln Tyr Ala Glu Thr Tyr Gly Phe Ile Val Ile Tyr Pro Glu Ser 
                85                  90                  95      


Pro Tyr Ser Gly Thr Cys Trp Asp Val Ser Ser Gln Ser Thr Leu Thr 
            100                 105                 110         


His Asn Gly Gly Gly Asn Ser Asn Ser Ile Ala Asn Met Val Asp Trp 
        115                 120                 125             


Thr Ile Asn Gln Tyr Asn Ala Asp Ala Ser Arg Val Tyr Val Thr Gly 
    130                 135                 140                 


Thr Ser Ser Gly Ala Met Met Thr Asn Val Met Ala Ala Thr Tyr Pro 
145                 150                 155                 160 


Asn Leu Phe Ala Ala Gly Ile Ala Tyr Ala Gly Val Pro Ala Gly Cys 
                165                 170                 175     


Phe Tyr Ser Glu Ala Asn Val Glu Asp Gln Trp Asn Ser Thr Cys Ala 
            180                 185                 190         


Gln Gly Gln Ser Ile Ser Thr Pro Glu His Trp Ala Gln Ile Ala Gln 
        195                 200                 205             


Ala Met Tyr Ser Gly Tyr Glu Gly Ser Arg Pro Lys Met Gln Ile Tyr 
    210                 215                 220                 


His Gly Ser Ala Asp Ala Thr Leu Tyr Pro Gln Asn Tyr Tyr Glu Thr 
225                 230                 235                 240 


Cys Lys Gln Trp Ala Gly Val Phe Gly Tyr Asn Tyr Asp Ser Pro Gln 
                245                 250                 255     


Glu Val Gln Asn Asp Thr Pro Val Ala Gly Trp Ala Lys Thr Ile Trp 
            260                 265                 270         


Gly Glu Asn Leu Gln Gly Ile Leu Ala Asp Gly Val Gly His Asn Ile 
        275                 280                 285             


Gln Ile Gln Gly Glu Glu Asp Leu Lys Trp Phe Gly Phe Thr Ser Trp 
    290                 295                 300                 


Ser His Pro Gln Phe Glu Lys 
305                 310     


<210>  5
<211>  1020
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Nucleotide sequence encoding tagged acetylxylan esterase 
       comprising the gametolysin signal peptide

<400>  5
atgtcgctgg cgacgcggcg cttcggcgcc gcagcggcgc ttctagtcgc cgcatgcgtg       60

ctgtgcacag ctcctgcgtg ggccgtgaag ctccagtacc tgctgtccat cctgctctac      120

gcgtactcct gcacggccct catgctggac cgccgcgacc cgacgcccgg gcagctctcc      180

caggtgaccg actttgggga taacccgacc aacgtcgggt tttacattta cgtgccccag      240

aacctggcct cgaacccggc catcattgtg gctattcatt actgcacggg cacggcccag      300

gcgtactact ccgggacccc ctacgcgcag tacgctgaga cgtacgggtt tatcgtcatc      360

taccccgagt ccccctactc ggggacgtgc tgggacgtct ccagccagag cacgctcacg      420

cacaacgggg ggggtaactc gaactcgatt gcgaacatgg tcgactggac gattaaccag      480

tacaacgcgg acgccagccg ggtctacgtc acgggcacgt cgagcggcgc gatgatgacc      540

aacgtcatgg ctgctaccta ccccaacctg tttgccgcgg ggatcgcgta cgcgggggtg      600

cccgctgggt gcttctacag cgaggcgaac gtcgaggacc agtggaacag cacctgcgct      660

caggggcaga gcatctccac gcctgagcat tgggcccaga tcgctcaggc tatgtactcc      720

ggctacgagg gctcccgccc caagatgcag atctaccacg ggtccgcgga cgcgacgctc      780

tacccccaga actactacga gacgtgcaag cagtgggctg gcgtcttcgg ctacaactac      840

gactcgcccc aggaggtcca gaacgatacg cctgtggctg gctgggccaa gaccatttgg      900

ggtgagaacc tccagggtat cctggccgac ggcgtcgggc acaacatcca gatccagggt      960

gaggaggatc tgaagtggtt cggttttacg agctggagcc acccgcagtt cgagaagtaa     1020


<210>  6
<211>  302
<212>  PRT
<213>  Trichoderma reesei

<400>  6

Met Pro Ser Val Lys Glu Thr Leu Thr Leu Leu Leu Ser Gln Ala Phe 
1               5                   10                  15      


Leu Ala Thr Gly Ser Pro Val Asp Gly Glu Thr Val Val Lys Arg Gln 
            20                  25                  30          


Cys Pro Ala Ile His Val Phe Gly Ala Arg Glu Thr Thr Val Ser Gln 
        35                  40                  45              


Gly Tyr Gly Ser Ser Ala Thr Val Val Asn Leu Val Ile Gln Ala His 
    50                  55                  60                  


Pro Gly Thr Thr Ser Glu Ala Ile Val Tyr Pro Ala Cys Gly Gly Gln 
65                  70                  75                  80  


Ala Ser Cys Gly Gly Ile Ser Tyr Ala Asn Ser Val Val Asn Gly Thr 
                85                  90                  95      


Asn Ala Ala Ala Ala Ala Ile Asn Asn Phe His Asn Ser Cys Pro Asp 
            100                 105                 110         


Thr Gln Leu Val Leu Val Gly Tyr Ser Gln Gly Ala Gln Ile Phe Asp 
        115                 120                 125             


Asn Ala Leu Cys Gly Gly Gly Asp Pro Gly Glu Gly Ile Thr Asn Thr 
    130                 135                 140                 


Ala Val Pro Leu Thr Ala Gly Ala Val Ser Ala Val Lys Ala Ala Ile 
145                 150                 155                 160 


Phe Met Gly Asp Pro Arg Asn Ile His Gly Leu Pro Tyr Asn Val Gly 
                165                 170                 175     


Thr Cys Thr Thr Gln Gly Phe Asp Ala Arg Pro Ala Gly Phe Val Cys 
            180                 185                 190         


Pro Ser Ala Ser Lys Ile Lys Ser Tyr Cys Asp Ala Ala Asp Pro Tyr 
        195                 200                 205             


Cys Cys Thr Gly Asn Asp Pro Asn Val His Gln Gly Tyr Gly Gln Glu 
    210                 215                 220                 


Tyr Gly Gln Gln Ala Leu Ala Phe Ile Asn Ser Gln Leu Ser Ser Gly 
225                 230                 235                 240 


Gly Ser Gln Pro Pro Gly Gly Gly Pro Thr Ser Thr Ser Arg Pro Thr 
                245                 250                 255     


Ser Thr Arg Thr Gly Ser Ser Pro Gly Pro Thr Gln Thr His Trp Gly 
            260                 265                 270         


Gln Cys Gly Gly Gln Gly Trp Thr Gly Pro Thr Gln Cys Glu Ser Gly 
        275                 280                 285             


Thr Thr Cys Gln Val Ile Ser Gln Trp Tyr Ser Gln Cys Leu 
    290                 295                 300         


<210>  7
<211>  382
<212>  PRT
<213>  Talaromyces purpureogenus

<400>  7

Met Lys Ser Leu Ser Phe Ser Phe Leu Val Thr Leu Phe Leu Tyr Leu 
1               5                   10                  15      


Thr Leu Ser Ser Ala Arg Thr Leu Gly Lys Asp Val Asn Lys Arg Val 
            20                  25                  30          


Thr Ala Gly Ser Leu Gln Gln Val Thr Gly Phe Gly Asp Asn Ala Ser 
        35                  40                  45              


Gly Thr Leu Met Tyr Ile Tyr Val Pro Lys Asn Leu Ala Thr Asn Pro 
    50                  55                  60                  


Gly Ile Val Val Ala Ile His Tyr Cys Thr Gly Thr Ala Gln Ala Tyr 
65                  70                  75                  80  


Tyr Thr Gly Ser Pro Tyr Ala Gln Leu Ala Glu Gln Tyr Gly Phe Ile 
                85                  90                  95      


Val Ile Tyr Pro Gln Ser Pro Tyr Ser Gly Thr Cys Trp Asp Val Ser 
            100                 105                 110         


Ser Gln Ala Ala Leu Thr His Asn Gly Gly Gly Asp Ser Asn Ser Ile 
        115                 120                 125             


Ala Asn Met Val Thr Trp Thr Ile Ser Gln Tyr Asn Ala Asn Thr Ala 
    130                 135                 140                 


Lys Val Phe Val Thr Gly Ser Ser Ser Gly Ala Met Met Thr Asn Val 
145                 150                 155                 160 


Met Ala Ala Thr Tyr Pro Glu Leu Phe Ala Ala Ala Thr Val Tyr Ser 
                165                 170                 175     


Gly Val Gly Ala Gly Cys Phe Tyr Ser Ser Ser Asn Gln Ala Asp Ala 
            180                 185                 190         


Trp Asn Ser Ser Cys Ala Thr Gly Ser Val Ile Ser Thr Pro Ala Val 
        195                 200                 205             


Trp Gly Gly Ile Ala Lys Asn Met Tyr Ser Gly Tyr Ser Gly Ser Arg 
    210                 215                 220                 


Pro Arg Met Gln Ile Tyr His Gly Ser Ala Asp Thr Thr Leu Tyr Pro 
225                 230                 235                 240 


Gln Asn Tyr Tyr Glu Thr Cys Lys Gln Trp Ala Gly Val Phe Gly Tyr 
                245                 250                 255     


Asn Tyr Asp Ser Pro Gln Ser Thr Leu Ala Asn Thr Pro Asp Ala Asn 
            260                 265                 270         


Tyr Gln Thr Thr Asn Trp Gly Pro Asn Leu Gln Gly Ile Tyr Ala Thr 
        275                 280                 285             


Gly Val Gly His Thr Val Pro Ile His Gly Ala Lys Asp Met Glu Trp 
    290                 295                 300                 


Phe Gly Phe Ser Gly Ser Gly Ser Ser Ser Thr Thr Thr Ala Ser Ala 
305                 310                 315                 320 


Thr Lys Thr Ser Thr Thr Ser Thr Thr Ser Thr Lys Thr Thr Ser Ser 
                325                 330                 335     


Thr Ser Ser Thr Thr Thr Ser Ser Thr Gly Val Ala Ala His Trp Gly 
            340                 345                 350         


Gln Cys Gly Gly Ser Gly Trp Thr Gly Pro Thr Val Cys Glu Ser Gly 
        355                 360                 365             


Tyr Thr Cys Thr Tyr Ser Asn Ala Trp Tyr Ser Gln Cys Leu 
    370                 375                 380         


<210>  8
<211>  346
<212>  PRT
<213>  Thermoanaerobacterium Sp.

<400>  8

Met Lys His His His His His His Pro Met Ser Asp Tyr Asp Ile Pro 
1               5                   10                  15      


Thr Thr Glu Asn Leu Tyr Phe Gln Gly Ala Met Gly Leu Phe Asp Met 
            20                  25                  30          


Pro Leu Gln Lys Leu Arg Glu Tyr Thr Gly Thr Asn Pro Cys Pro Glu 
        35                  40                  45              


Asp Phe Asp Glu Tyr Trp Asn Arg Ala Leu Asp Glu Met Arg Ser Val 
    50                  55                  60                  


Asp Pro Lys Ile Glu Leu Lys Glu Ser Ser Phe Gln Val Ser Phe Ala 
65                  70                  75                  80  


Glu Cys Tyr Asp Leu Tyr Phe Thr Gly Val Arg Gly Ala Arg Ile His 
                85                  90                  95      


Ala Lys Tyr Ile Lys Pro Lys Thr Glu Gly Lys His Pro Ala Leu Ile 
            100                 105                 110         


Arg Phe His Gly Tyr Ser Ser Asn Ser Gly Asp Trp Asn Asp Lys Leu 
        115                 120                 125             


Asn Tyr Val Ala Ala Gly Phe Thr Val Val Ala Met Asp Val Arg Gly 
    130                 135                 140                 


Gln Gly Gly Gln Ser Gln Asp Val Gly Gly Val Thr Gly Asn Thr Leu 
145                 150                 155                 160 


Asn Gly His Ile Ile Arg Gly Leu Asp Asp Asp Ala Asp Asn Met Leu 
                165                 170                 175     


Phe Arg His Ile Phe Leu Asp Thr Ala Gln Leu Ala Gly Ile Val Met 
            180                 185                 190         


Asn Met Pro Glu Val Asp Glu Asp Arg Val Gly Val Met Gly Pro Ser 
        195                 200                 205             


Gln Gly Gly Gly Leu Ser Leu Ala Cys Ala Ala Leu Glu Pro Arg Val 
    210                 215                 220                 


Arg Lys Val Val Ser Glu Tyr Pro Phe Leu Ser Asp Tyr Lys Arg Val 
225                 230                 235                 240 


Trp Asp Leu Asp Leu Ala Lys Asn Ala Tyr Gln Glu Ile Thr Asp Tyr 
                245                 250                 255     


Phe Arg Leu Phe Asp Pro Arg His Glu Arg Glu Asn Glu Val Phe Thr 
            260                 265                 270         


Lys Leu Gly Tyr Ile Asp Val Lys Asn Leu Ala Lys Arg Ile Lys Gly 
        275                 280                 285             


Asp Val Leu Met Cys Val Gly Leu Met Asp Gln Val Cys Pro Pro Ser 
    290                 295                 300                 


Thr Val Phe Ala Ala Tyr Asn Asn Ile Gln Ser Lys Lys Asp Ile Lys 
305                 310                 315                 320 


Val Tyr Pro Asp Tyr Gly His Glu Pro Met Arg Gly Phe Gly Asp Leu 
                325                 330                 335     


Ala Met Gln Phe Met Leu Glu Leu Tyr Ser 
            340                 345     


<210>  9
<211>  28
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Gametolysin signal peptide

<400>  9

Met Ser Leu Ala Thr Arg Arg Phe Gly Ala Ala Ala Ala Leu Leu Val 
1               5                   10                  15      


Ala Ala Cys Val Leu Cys Thr Ala Pro Ala Trp Ala 
            20                  25              


