                         SEQUENCE LISTING

<110>  Universitt fr Bodenkultur
 
<120>  Production of polysialylated polypeptides in plants and plant 
       cells

<130>  49181

<150>  EP 16193002.9
<151>  2016-10-10

<160>  38    

<170>  PatentIn version 3.5

<210>  1
<211>  29
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer

<400>  1
cgcgttaata cctaggccat gggccatgg                                         29


<210>  2
<211>  29
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer

<400>  2
cgcgccatgg cccatggcct aggtattaa                                         29


<210>  3
<211>  33
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer

<400>  3
agcaaggaga tggttctagt gatgcggaag aag                                    33


<210>  4
<211>  33
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer

<400>  4
cttcttccgc atcactagaa ccatctcctt gct                                    33


<210>  5
<211>  33
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer

<400>  5
ccataaattc tagaggcgca tcgcggccgc tcc                                    33


<210>  6
<211>  33
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer

<400>  6
ggagcggccg cgatgcgcct ctagaattta tgg                                    33


<210>  7
<211>  33
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer

<400>  7
tatatctaga atggtcagca agtcccgctg gaa                                    33


<210>  8
<211>  33
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer

<400>  8
tataggatcc tcagaaggac gtgaggttct tga                                    33


<210>  9
<211>  31
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer

<400>  9
tatatctaga atgcagctgc agttccggag c                                      31


<210>  10
<211>  57
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer

<400>  10
tataagatct ttacttttcg aactgcggat ggctccacgt ggccccatcg cactggc          57


<210>  11
<211>  30
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer

<400>  11
tataagatct cgtggcccca tcgcactggc                                        30


<210>  12
<211>  30
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer

<400>  12
tatatctaga atgcgctcca ttaggaagag                                        30


<210>  13
<211>  55
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer

<400>  13
tataggatcc ttacttttcg aactgcggat ggctccattg ctttacacac tttcc            55


<210>  14
<211>  28
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer

<400>  14
tataggatcc ttgctttaca cactttcc                                          28


<210>  15
<211>  66
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  CTS region

<400>  15

Met Gly Val Phe Ser Asn Leu Arg Gly Pro Lys Ile Gly Leu Thr His 
1               5                   10                  15      


Glu Glu Leu Pro Val Val Ala Asn Gly Ser Thr Ser Ser Ser Ser Ser 
            20                  25                  30          


Pro Ser Ser Phe Lys Arg Lys Val Ser Thr Phe Leu Pro Ile Cys Val 
        35                  40                  45              


Ala Leu Val Val Ile Ile Glu Ile Gly Phe Leu Cys Arg Leu Asp Asn 
    50                  55                  60                  


Ala Ser 
65      


<210>  16
<211>  66
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  CTS region

<400>  16

Met Gly Val Phe Ser Asn Leu Arg Gly Pro Arg Ala Gly Ala Thr His 
1               5                   10                  15      


Asp Glu Phe Pro Ala Thr Asn Gly Ser Pro Ser Ser Ser Ser Ser Pro 
            20                  25                  30          


Ser Ser Ser Ile Lys Arg Lys Leu Ser Asn Leu Leu Pro Leu Cys Val 
        35                  40                  45              


Ala Leu Val Val Ile Ala Glu Ile Gly Phe Leu Gly Arg Leu Asp Lys 
    50                  55                  60                  


Val Ala 
65      


<210>  17
<211>  52
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  CTS region

<400>  17

Met Pro Met Arg Tyr Leu Asn Ala Met Ala Ala Leu Leu Met Met Phe 
1               5                   10                  15      


Phe Thr Leu Leu Ile Leu Ser Phe Thr Gly Ile Leu Glu Phe Pro Ser 
            20                  25                  30          


Ala Ser Thr Ser Met Glu His Ser Ile Asp Pro Glu Pro Lys Leu Ser 
        35                  40                  45              


Asp Ser Thr Ser 
    50          


<210>  18
<211>  90
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  CTS region

<400>  18

Met Ser Lys Arg Asn Pro Lys Ile Leu Lys Ile Phe Leu Tyr Met Leu 
1               5                   10                  15      


Leu Leu Asn Ser Leu Phe Leu Ile Ile Tyr Phe Val Phe His Ser Ser 
            20                  25                  30          


Ser Phe Ser Pro Glu Gln Ser Gln Pro Pro His Ile Tyr His Val Ser 
        35                  40                  45              


Val Asn Asn Gln Ser Ala Ile Gln Lys Pro Trp Pro Ile Leu Pro Ser 
    50                  55                  60                  


Tyr Leu Pro Trp Thr Pro Pro Gln Arg Asn Leu Pro Thr Gly Ser Cys 
65                  70                  75                  80  


Glu Gly Tyr Phe Gly Asn Gly Phe Thr Lys 
                85                  90  


<210>  19
<211>  76
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  CTS region

<400>  19

Met Ala Asn Leu Trp Lys Lys Gln Arg Leu Arg Asp Thr Gly Leu Cys 
1               5                   10                  15      


Arg Leu Gly Ile Leu Phe Ala Val Thr Leu Ser Ile Val Leu Met Leu 
            20                  25                  30          


Val Ser Val Pro Arg Thr Ala Leu Asn Gly Ser Ser Ile Asp Asp Asp 
        35                  40                  45              


Leu Asp Gly Leu Asp Lys Asp Leu Glu Ala Lys Leu Asn Ala Ser Leu 
    50                  55                  60                  


Leu Ser Val Ala Arg Gly Asn Arg Met Ser Leu Arg 
65                  70                  75      


<210>  20
<211>  60
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  CTS region

<400>  20

Met Lys Arg Phe Tyr Gly Gly Leu Leu Val Val Ser Met Cys Met Phe 
1               5                   10                  15      


Leu Thr Val Tyr Arg Tyr Val Asp Leu Asn Thr Pro Val Glu Lys Pro 
            20                  25                  30          


Tyr Ile Thr Ala Ala Ala Ser Val Val Val Thr Pro Asn Thr Thr Leu 
        35                  40                  45              


Pro Met Glu Trp Leu Arg Ile Thr Leu Pro Asp Phe 
    50                  55                  60  


<210>  21
<211>  118
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  CTS region

<400>  21

Met Lys Gln Phe Met Ser Val Val Arg Phe Lys Phe Gly Phe Thr Ser 
1               5                   10                  15      


Val Arg Met Arg Asp Trp Ser Val Gly Val Ser Ile Met Val Leu Thr 
            20                  25                  30          


Leu Ile Phe Ile Ile Arg Tyr Glu Gln Ser Asp His Thr His Thr Val 
        35                  40                  45              


Asp Asp Ser Ser Ile Glu Gly Glu Ser Val His Glu Pro Ala Lys Lys 
    50                  55                  60                  


Pro His Phe Met Thr Leu Glu Asp Leu Asp Tyr Leu Phe Ser Asn Lys 
65                  70                  75                  80  


Ser Phe Phe Gly Glu Glu Glu Val Ser Asn Gly Met Leu Val Trp Ser 
                85                  90                  95      


Arg Met Arg Pro Phe Leu Glu Arg Pro Asp Ala Leu Pro Glu Thr Ala 
            100                 105                 110         


Gln Gly Ile Glu Glu Ala 
        115             


<210>  22
<211>  52
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  CTS region

<400>  22

Met Ile His Thr Asn Leu Lys Lys Lys Phe Ser Leu Phe Ile Leu Val 
1               5                   10                  15      


Phe Leu Leu Phe Ala Val Ile Cys Val Trp Lys Lys Gly Ser Asp Tyr 
            20                  25                  30          


Glu Ala Leu Thr Leu Gln Ala Lys Glu Phe Gln Met Pro Lys Ser Gln 
        35                  40                  45              


Glu Lys Val Ala 
    50          


<210>  23
<211>  51
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  CTS region

<400>  23

Met Ile His Thr Asn Leu Lys Lys Lys Phe Ser Cys Cys Val Leu Val 
1               5                   10                  15      


Phe Leu Leu Phe Ala Val Ile Cys Val Trp Lys Glu Lys Lys Lys Gly 
            20                  25                  30          


Ser Tyr Tyr Asp Ser Phe Lys Leu Thr Lys Glu Phe Gln Val Leu Lys 
        35                  40                  45              


Ser Leu Gly 
    50      


<210>  24
<211>  52
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  CTS region

<400>  24

Met Val Ser Lys Ser Arg Trp Lys Leu Leu Ala Met Leu Ala Leu Val 
1               5                   10                  15      


Leu Val Val Met Val Trp Tyr Ser Ile Ser Arg Glu Asp Arg Tyr Ile 
            20                  25                  30          


Glu Leu Phe Tyr Phe Pro Ile Pro Glu Lys Lys Glu Pro Cys Leu Gln 
        35                  40                  45              


Gly Glu Ala Glu 
    50          


<210>  25
<211>  414
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ST6-ST8Sia-II

<400>  25

Met Ile His Thr Asn Leu Lys Lys Lys Phe Ser Leu Phe Ile Leu Val 
1               5                   10                  15      


Phe Leu Leu Phe Ala Val Ile Cys Val Trp Lys Lys Gly Ser Asp Tyr 
            20                  25                  30          


Glu Ala Leu Thr Leu Gln Ala Lys Glu Phe Gln Met Pro Lys Ser Gln 
        35                  40                  45              


Glu Lys Val Ala Leu Glu Asp Ile Ser Glu Ile Glu Glu Glu Ile Gly 
    50                  55                  60                  


Asn Ser Gly Gly Arg Gly Thr Ile Arg Ser Ala Val Asn Ser Leu His 
65                  70                  75                  80  


Ser Lys Ser Asn Arg Ala Glu Val Val Ile Asn Gly Ser Ser Ser Pro 
                85                  90                  95      


Ala Val Val Asp Arg Ser Asn Glu Ser Ile Lys His Asn Ile Gln Pro 
            100                 105                 110         


Ala Ser Ser Lys Trp Arg His Asn Gln Thr Leu Ser Leu Arg Ile Arg 
        115                 120                 125             


Lys Gln Ile Leu Lys Phe Leu Asp Ala Glu Lys Asp Ile Ser Val Leu 
    130                 135                 140                 


Lys Gly Thr Leu Lys Pro Gly Asp Ile Ile His Tyr Ile Phe Asp Arg 
145                 150                 155                 160 


Asp Ser Thr Met Asn Val Ser Gln Asn Leu Tyr Glu Leu Leu Pro Arg 
                165                 170                 175     


Thr Ser Pro Leu Lys Asn Lys His Phe Gly Thr Cys Ala Ile Val Gly 
            180                 185                 190         


Asn Ser Gly Val Leu Leu Asn Ser Gly Cys Gly Gln Glu Ile Asp Ala 
        195                 200                 205             


His Ser Phe Val Ile Arg Cys Asn Leu Ala Pro Val Gln Glu Tyr Ala 
    210                 215                 220                 


Arg Asp Val Gly Leu Lys Thr Asp Leu Val Thr Met Asn Pro Ser Val 
225                 230                 235                 240 


Ile Gln Arg Ala Phe Glu Asp Leu Val Asn Ala Thr Trp Arg Glu Lys 
                245                 250                 255     


Leu Leu Gln Arg Leu His Ser Leu Asn Gly Ser Ile Leu Trp Ile Pro 
            260                 265                 270         


Ala Phe Met Ala Arg Gly Gly Lys Glu Arg Val Glu Trp Val Asn Glu 
        275                 280                 285             


Leu Ile Leu Lys His His Val Asn Val Arg Thr Ala Tyr Pro Ser Leu 
    290                 295                 300                 


Arg Leu Leu His Ala Val Arg Gly Tyr Trp Leu Thr Asn Lys Val His 
305                 310                 315                 320 


Ile Lys Arg Pro Thr Thr Gly Leu Leu Met Tyr Thr Leu Ala Thr Arg 
                325                 330                 335     


Phe Cys Lys Gln Ile Tyr Leu Tyr Gly Phe Trp Pro Phe Pro Leu Asp 
            340                 345                 350         


Gln Asn Gln Asn Pro Val Lys Tyr His Tyr Tyr Asp Ser Leu Lys Tyr 
        355                 360                 365             


Gly Tyr Thr Ser Gln Ala Ser Pro His Thr Met Pro Leu Glu Phe Lys 
    370                 375                 380                 


Ala Leu Lys Ser Leu His Glu Gln Gly Ala Leu Lys Leu Thr Val Gly 
385                 390                 395                 400 


Gln Cys Asp Gly Ala Thr Trp Ser His Pro Gln Phe Glu Lys 
                405                 410                 


<210>  26
<211>  395
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ST6-ST8sia-IV

<400>  26

Met Ile His Thr Asn Leu Lys Lys Lys Phe Ser Leu Phe Ile Leu Val 
1               5                   10                  15      


Phe Leu Leu Phe Ala Val Ile Cys Val Trp Lys Lys Gly Ser Asp Tyr 
            20                  25                  30          


Glu Ala Leu Thr Leu Gln Ala Lys Glu Phe Gln Met Pro Lys Ser Gln 
        35                  40                  45              


Glu Lys Val Ala Leu Glu Arg Thr Glu Glu His Gln Glu Thr Gln Leu 
    50                  55                  60                  


Ile Gly Asp Gly Glu Leu Ser Leu Ser Arg Ser Leu Val Asn Ser Ser 
65                  70                  75                  80  


Asp Lys Ile Ile Arg Lys Ala Gly Ser Ser Ile Phe Gln His Asn Val 
                85                  90                  95      


Glu Gly Trp Lys Ile Asn Ser Ser Leu Val Leu Glu Ile Arg Lys Asn 
            100                 105                 110         


Ile Leu Arg Phe Leu Asp Ala Glu Arg Asp Val Ser Val Val Lys Ser 
        115                 120                 125             


Ser Phe Lys Pro Gly Asp Val Ile His Tyr Val Leu Asp Arg Arg Arg 
    130                 135                 140                 


Thr Leu Asn Ile Ser His Asp Leu His Ser Leu Leu Pro Glu Val Ser 
145                 150                 155                 160 


Pro Met Lys Asn Arg Arg Phe Lys Thr Cys Ala Val Val Gly Asn Ser 
                165                 170                 175     


Gly Ile Leu Leu Asp Ser Glu Cys Gly Lys Glu Ile Asp Ser His Asn 
            180                 185                 190         


Phe Val Ile Arg Cys Asn Leu Ala Pro Val Val Glu Phe Ala Ala Asp 
        195                 200                 205             


Val Gly Thr Lys Ser Asp Phe Ile Thr Met Asn Pro Ser Val Val Gln 
    210                 215                 220                 


Arg Ala Phe Gly Gly Phe Arg Asn Glu Ser Asp Arg Glu Lys Phe Val 
225                 230                 235                 240 


His Arg Leu Ser Met Leu Asn Asp Ser Val Leu Trp Ile Pro Ala Phe 
                245                 250                 255     


Met Val Lys Gly Gly Glu Lys His Val Glu Trp Val Asn Ala Leu Ile 
            260                 265                 270         


Leu Lys Asn Lys Leu Lys Val Arg Thr Ala Tyr Pro Ser Leu Arg Leu 
        275                 280                 285             


Ile His Ala Val Arg Gly Tyr Trp Leu Thr Asn Lys Val Pro Ile Lys 
    290                 295                 300                 


Arg Pro Ser Thr Gly Leu Leu Met Tyr Thr Leu Ala Thr Arg Phe Cys 
305                 310                 315                 320 


Asp Glu Ile His Leu Tyr Gly Phe Trp Pro Phe Pro Lys Asp Leu Asn 
                325                 330                 335     


Gly Lys Ala Val Lys Tyr His Tyr Tyr Asp Asp Leu Lys Tyr Arg Tyr 
            340                 345                 350         


Phe Ser Asn Ala Ser Pro His Arg Met Pro Leu Glu Phe Lys Thr Leu 
        355                 360                 365             


Asn Val Leu His Asn Arg Gly Ala Leu Lys Leu Thr Thr Gly Lys Cys 
    370                 375                 380                 


Val Lys Gln Trp Ser His Pro Gln Phe Glu Lys 
385                 390                 395 


<210>  27
<211>  22
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Tobacco chitinase signal peptide sequence

<400>  27

Met Lys Thr Asn Leu Phe Leu Phe Leu Ile Phe Ser Leu Leu Leu Ser 
1               5                   10                  15      


Leu Ser Ser Ala Glu Phe 
            20          


<210>  28
<211>  20
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Arabidopsis alpha-glucosidase II signal peptide sequence

<400>  28

Met Arg Ser Leu Leu Phe Val Leu Ser Leu Ile Cys Phe Cys Ser Gln 
1               5                   10                  15      


Thr Ala Leu Ser 
            20  


<210>  29
<211>  24
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Barley alpha-amylase signal peptide sequence

<400>  29

Met Ala Asn Lys His Leu Ser Leu Ser Leu Phe Leu Val Leu Leu Gly 
1               5                   10                  15      


Leu Ser Ala Ser Leu Ala Ser Gly 
            20                  


<210>  30
<211>  27
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  N. plumbaginifolia calreticulin signal peptide sequence

<400>  30

Met Ala Thr Gln Arg Arg Ala Asn Pro Ser Ser Leu His Leu Ile Thr 
1               5                   10                  15      


Val Phe Ser Leu Leu Val Ala Val Val Ser Ala 
            20                  25          


<210>  31
<211>  386
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Secreted ST8Sia-II

<400>  31

Met Ala Asn Lys His Leu Ser Leu Ser Leu Phe Leu Val Leu Leu Gly 
1               5                   10                  15      


Leu Ser Ala Ser Leu Ala Ser Gly Asp Ile Ser Glu Ile Glu Glu Glu 
            20                  25                  30          


Ile Gly Asn Ser Gly Gly Arg Gly Thr Ile Arg Ser Ala Val Asn Ser 
        35                  40                  45              


Leu His Ser Lys Ser Asn Arg Ala Glu Val Val Ile Asn Gly Ser Ser 
    50                  55                  60                  


Ser Pro Ala Val Val Asp Arg Ser Asn Glu Ser Ile Lys His Asn Ile 
65                  70                  75                  80  


Gln Pro Ala Ser Ser Lys Trp Arg His Asn Gln Thr Leu Ser Leu Arg 
                85                  90                  95      


Ile Arg Lys Gln Ile Leu Lys Phe Leu Asp Ala Glu Lys Asp Ile Ser 
            100                 105                 110         


Val Leu Lys Gly Thr Leu Lys Pro Gly Asp Ile Ile His Tyr Ile Phe 
        115                 120                 125             


Asp Arg Asp Ser Thr Met Asn Val Ser Gln Asn Leu Tyr Glu Leu Leu 
    130                 135                 140                 


Pro Arg Thr Ser Pro Leu Lys Asn Lys His Phe Gly Thr Cys Ala Ile 
145                 150                 155                 160 


Val Gly Asn Ser Gly Val Leu Leu Asn Ser Gly Cys Gly Gln Glu Ile 
                165                 170                 175     


Asp Ala His Ser Phe Val Ile Arg Cys Asn Leu Ala Pro Val Gln Glu 
            180                 185                 190         


Tyr Ala Arg Asp Val Gly Leu Lys Thr Asp Leu Val Thr Met Asn Pro 
        195                 200                 205             


Ser Val Ile Gln Arg Ala Phe Glu Asp Leu Val Asn Ala Thr Trp Arg 
    210                 215                 220                 


Glu Lys Leu Leu Gln Arg Leu His Ser Leu Asn Gly Ser Ile Leu Trp 
225                 230                 235                 240 


Ile Pro Ala Phe Met Ala Arg Gly Gly Lys Glu Arg Val Glu Trp Val 
                245                 250                 255     


Asn Glu Leu Ile Leu Lys His His Val Asn Val Arg Thr Ala Tyr Pro 
            260                 265                 270         


Ser Leu Arg Leu Leu His Ala Val Arg Gly Tyr Trp Leu Thr Asn Lys 
        275                 280                 285             


Val His Ile Lys Arg Pro Thr Thr Gly Leu Leu Met Tyr Thr Leu Ala 
    290                 295                 300                 


Thr Arg Phe Cys Lys Gln Ile Tyr Leu Tyr Gly Phe Trp Pro Phe Pro 
305                 310                 315                 320 


Leu Asp Gln Asn Gln Asn Pro Val Lys Tyr His Tyr Tyr Asp Ser Leu 
                325                 330                 335     


Lys Tyr Gly Tyr Thr Ser Gln Ala Ser Ala His Thr Met Pro Leu Glu 
            340                 345                 350         


Phe Lys Ala Leu Lys Ser Leu His Glu Gln Gly Ala Leu Lys Leu Thr 
        355                 360                 365             


Val Gly Gln Cys Asp Gly Ala Thr Arg Ser Trp Ser His Pro Gln Phe 
    370                 375                 380                 


Glu Lys 
385     


<210>  32
<211>  367
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Secreted ST8Sia-IV

<400>  32

Met Ala Asn Lys His Leu Ser Leu Ser Leu Phe Leu Val Leu Leu Gly 
1               5                   10                  15      


Leu Ser Ala Ser Leu Ala Ser Gly Arg Thr Glu Glu His Gln Glu Thr 
            20                  25                  30          


Gln Leu Ile Gly Asp Gly Glu Leu Ser Leu Ser Arg Ser Leu Val Asn 
        35                  40                  45              


Ser Ser Asp Lys Ile Ile Arg Lys Ala Gly Ser Ser Ile Phe Gln His 
    50                  55                  60                  


Asn Val Glu Gly Trp Lys Ile Asn Ser Ser Leu Val Leu Glu Ile Arg 
65                  70                  75                  80  


Lys Asn Ile Leu Arg Phe Leu Asp Ala Glu Arg Asp Val Ser Val Val 
                85                  90                  95      


Lys Ser Ser Phe Lys Pro Gly Asp Val Ile His Tyr Val Leu Asp Arg 
            100                 105                 110         


Arg Arg Thr Leu Asn Ile Ser His Asp Leu His Ser Leu Leu Pro Glu 
        115                 120                 125             


Val Ser Pro Met Lys Asn Arg Arg Phe Lys Thr Cys Ala Val Val Gly 
    130                 135                 140                 


Asn Ser Gly Ile Leu Leu Asp Ser Glu Cys Gly Lys Glu Ile Asp Ser 
145                 150                 155                 160 


His Asn Phe Val Ile Arg Cys Asn Leu Ala Pro Val Val Glu Phe Ala 
                165                 170                 175     


Ala Asp Val Gly Thr Lys Ser Asp Phe Ile Thr Met Asn Pro Ser Val 
            180                 185                 190         


Val Gln Arg Ala Phe Gly Gly Phe Arg Asn Glu Ser Asp Arg Glu Lys 
        195                 200                 205             


Phe Val His Arg Leu Ser Met Leu Asn Asp Ser Val Leu Trp Ile Pro 
    210                 215                 220                 


Ala Phe Met Val Lys Gly Gly Glu Lys His Val Glu Trp Val Asn Ala 
225                 230                 235                 240 


Leu Ile Leu Lys Asn Lys Leu Lys Val Arg Thr Ala Tyr Pro Ser Leu 
                245                 250                 255     


Arg Leu Ile His Ala Val Arg Gly Tyr Trp Leu Thr Asn Lys Val Pro 
            260                 265                 270         


Ile Lys Arg Pro Ser Thr Gly Leu Leu Met Tyr Thr Leu Ala Thr Arg 
        275                 280                 285             


Phe Cys Asp Glu Ile His Leu Tyr Gly Phe Trp Pro Phe Pro Lys Asp 
    290                 295                 300                 


Leu Asn Gly Lys Ala Val Lys Tyr His Tyr Tyr Asp Asp Leu Lys Tyr 
305                 310                 315                 320 


Arg Tyr Phe Ser Asn Ala Ser Pro His Arg Met Pro Leu Glu Phe Lys 
                325                 330                 335     


Thr Leu Asn Val Leu His Asn Arg Gly Ala Leu Lys Leu Thr Thr Gly 
            340                 345                 350         


Lys Cys Val Lys Gln Gly Ser Trp Ser His Pro Gln Phe Glu Lys 
        355                 360                 365         


<210>  33
<211>  385
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ST8Sia-II

<400>  33

Met Gln Leu Gln Phe Arg Ser Trp Met Leu Ala Ala Leu Thr Leu Leu 
1               5                   10                  15      


Val Val Phe Leu Ile Phe Ala Asp Ile Ser Glu Ile Glu Glu Glu Ile 
            20                  25                  30          


Gly Asn Ser Gly Gly Arg Gly Thr Ile Arg Ser Ala Val Asn Ser Leu 
        35                  40                  45              


His Ser Lys Ser Asn Arg Ala Glu Val Val Ile Asn Gly Ser Ser Ser 
    50                  55                  60                  


Pro Ala Val Val Asp Arg Ser Asn Glu Ser Ile Lys His Asn Ile Gln 
65                  70                  75                  80  


Pro Ala Ser Ser Lys Trp Arg His Asn Gln Thr Leu Ser Leu Arg Ile 
                85                  90                  95      


Arg Lys Gln Ile Leu Lys Phe Leu Asp Ala Glu Lys Asp Ile Ser Val 
            100                 105                 110         


Leu Lys Gly Thr Leu Lys Pro Gly Asp Ile Ile His Tyr Ile Phe Asp 
        115                 120                 125             


Arg Asp Ser Thr Met Asn Val Ser Gln Asn Leu Tyr Glu Leu Leu Pro 
    130                 135                 140                 


Arg Thr Ser Pro Leu Lys Asn Lys His Phe Gly Thr Cys Ala Ile Val 
145                 150                 155                 160 


Gly Asn Ser Gly Val Leu Leu Asn Ser Gly Cys Gly Gln Glu Ile Asp 
                165                 170                 175     


Ala His Ser Phe Val Ile Arg Cys Asn Leu Ala Pro Val Gln Glu Tyr 
            180                 185                 190         


Ala Arg Asp Val Gly Leu Lys Thr Asp Leu Val Thr Met Asn Pro Ser 
        195                 200                 205             


Val Ile Gln Arg Ala Phe Glu Asp Leu Val Asn Ala Thr Trp Arg Glu 
    210                 215                 220                 


Lys Leu Leu Gln Arg Leu His Ser Leu Asn Gly Ser Ile Leu Trp Ile 
225                 230                 235                 240 


Pro Ala Phe Met Ala Arg Gly Gly Lys Glu Arg Val Glu Trp Val Asn 
                245                 250                 255     


Glu Leu Ile Leu Lys His His Val Asn Val Arg Thr Ala Tyr Pro Ser 
            260                 265                 270         


Leu Arg Leu Leu His Ala Val Arg Gly Tyr Trp Leu Thr Asn Lys Val 
        275                 280                 285             


His Ile Lys Arg Pro Thr Thr Gly Leu Leu Met Tyr Thr Leu Ala Thr 
    290                 295                 300                 


Arg Phe Cys Lys Gln Ile Tyr Leu Tyr Gly Phe Trp Pro Phe Pro Leu 
305                 310                 315                 320 


Asp Gln Asn Gln Asn Pro Val Lys Tyr His Tyr Tyr Asp Ser Leu Lys 
                325                 330                 335     


Tyr Gly Tyr Thr Ser Gln Ala Ser Ala His Thr Met Pro Leu Glu Phe 
            340                 345                 350         


Lys Ala Leu Lys Ser Leu His Glu Gln Gly Ala Leu Lys Leu Thr Val 
        355                 360                 365             


Gly Gln Cys Asp Gly Ala Thr Arg Ser Trp Ser His Pro Gln Phe Glu 
    370                 375                 380                 


Lys 
385 


<210>  34
<211>  369
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ST8Sia-IV

<400>  34

Met Arg Ser Ile Arg Lys Arg Trp Thr Ile Cys Thr Ile Ser Leu Leu 
1               5                   10                  15      


Leu Ile Phe Tyr Lys Thr Lys Glu Ile Ala Arg Thr Glu Glu His Gln 
            20                  25                  30          


Glu Thr Gln Leu Ile Gly Asp Gly Glu Leu Ser Leu Ser Arg Ser Leu 
        35                  40                  45              


Val Asn Ser Ser Asp Lys Ile Ile Arg Lys Ala Gly Ser Ser Ile Phe 
    50                  55                  60                  


Gln His Asn Val Glu Gly Trp Lys Ile Asn Ser Ser Leu Val Leu Glu 
65                  70                  75                  80  


Ile Arg Lys Asn Ile Leu Arg Phe Leu Asp Ala Glu Arg Asp Val Ser 
                85                  90                  95      


Val Val Lys Ser Ser Phe Lys Pro Gly Asp Val Ile His Tyr Val Leu 
            100                 105                 110         


Asp Arg Arg Arg Thr Leu Asn Ile Ser His Asp Leu His Ser Leu Leu 
        115                 120                 125             


Pro Glu Val Ser Pro Met Lys Asn Arg Arg Phe Lys Thr Cys Ala Val 
    130                 135                 140                 


Val Gly Asn Ser Gly Ile Leu Leu Asp Ser Glu Cys Gly Lys Glu Ile 
145                 150                 155                 160 


Asp Ser His Asn Phe Val Ile Arg Cys Asn Leu Ala Pro Val Val Glu 
                165                 170                 175     


Phe Ala Ala Asp Val Gly Thr Lys Ser Asp Phe Ile Thr Met Asn Pro 
            180                 185                 190         


Ser Val Val Gln Arg Ala Phe Gly Gly Phe Arg Asn Glu Ser Asp Arg 
        195                 200                 205             


Glu Lys Phe Val His Arg Leu Ser Met Leu Asn Asp Ser Val Leu Trp 
    210                 215                 220                 


Ile Pro Ala Phe Met Val Lys Gly Gly Glu Lys His Val Glu Trp Val 
225                 230                 235                 240 


Asn Ala Leu Ile Leu Lys Asn Lys Leu Lys Val Arg Thr Ala Tyr Pro 
                245                 250                 255     


Ser Leu Arg Leu Ile His Ala Val Arg Gly Tyr Trp Leu Thr Asn Lys 
            260                 265                 270         


Val Pro Ile Lys Arg Pro Ser Thr Gly Leu Leu Met Tyr Thr Leu Ala 
        275                 280                 285             


Thr Arg Phe Cys Asp Glu Ile His Leu Tyr Gly Phe Trp Pro Phe Pro 
    290                 295                 300                 


Lys Asp Leu Asn Gly Lys Ala Val Lys Tyr His Tyr Tyr Asp Asp Leu 
305                 310                 315                 320 


Lys Tyr Arg Tyr Phe Ser Asn Ala Ser Pro His Arg Met Pro Leu Glu 
                325                 330                 335     


Phe Lys Thr Leu Asn Val Leu His Asn Arg Gly Ala Leu Lys Leu Thr 
            340                 345                 350         


Thr Gly Lys Cys Val Lys Gln Gly Ser Trp Ser His Pro Gln Phe Glu 
        355                 360                 365             


Lys 
    


<210>  35
<211>  529
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ST6-PSTNmB

<400>  35

Met Ile His Thr Asn Leu Lys Lys Lys Phe Ser Leu Phe Ile Leu Val 
1               5                   10                  15      


Phe Leu Leu Phe Ala Val Ile Cys Val Trp Lys Lys Gly Ser Asp Tyr 
            20                  25                  30          


Glu Ala Leu Thr Leu Gln Ala Lys Glu Phe Gln Met Pro Lys Ser Gln 
        35                  40                  45              


Glu Lys Val Ala Leu Glu Trp Leu Thr Thr Ser Pro Phe Tyr Leu Thr 
    50                  55                  60                  


Pro Pro Arg Asn Asn Leu Phe Val Ile Ser Asn Leu Gly Gln Leu Asn 
65                  70                  75                  80  


Gln Val Gln Ser Leu Ile Lys Ile Gln Lys Leu Thr Asn Asn Leu Leu 
                85                  90                  95      


Val Ile Leu Tyr Thr Ser Gln Asn Leu Lys Met Pro Lys Leu Val His 
            100                 105                 110         


Gln Ser Ala Asn Lys Asn Leu Phe Glu Ser Ile Tyr Leu Phe Glu Leu 
        115                 120                 125             


Pro Arg Ser Pro Asn Asn Ile Thr Pro Lys Lys Leu Leu Tyr Ile Tyr 
    130                 135                 140                 


Arg Ser Tyr Lys Lys Ile Leu Asn Ile Ile Gln Pro Ala His Leu Tyr 
145                 150                 155                 160 


Met Leu Ser Phe Thr Gly His Tyr Ser Tyr Leu Ile Ser Ile Ala Lys 
                165                 170                 175     


Lys Lys Asn Ile Thr Thr His Leu Ile Asp Glu Gly Thr Gly Thr Tyr 
            180                 185                 190         


Ala Pro Leu Leu Glu Ser Phe Ser Tyr His Pro Thr Lys Leu Glu Arg 
        195                 200                 205             


Tyr Leu Ile Gly Asn Asn Leu Asn Ile Lys Gly Tyr Ile Asp His Phe 
    210                 215                 220                 


Asp Ile Leu His Val Pro Phe Pro Glu Tyr Ala Lys Lys Ile Phe Asn 
225                 230                 235                 240 


Ala Lys Lys Tyr Asn Arg Phe Phe Ala His Ala Gly Gly Ile Ser Ile 
                245                 250                 255     


Asn Asn Asn Ile Ala Asn Leu Gln Lys Lys Tyr Gln Ile Ser Lys Asn 
            260                 265                 270         


Asp Tyr Ile Phe Val Ser Gln Arg Tyr Pro Ile Ser Asp Asp Leu Tyr 
        275                 280                 285             


Tyr Lys Ser Ile Val Glu Ile Leu Asn Ser Ile Ser Leu Gln Ile Lys 
    290                 295                 300                 


Gly Lys Ile Phe Ile Lys Leu His Pro Lys Glu Met Gly Asn Asn Tyr 
305                 310                 315                 320 


Val Met Ser Leu Phe Leu Asn Met Val Glu Ile Asn Pro Arg Leu Val 
                325                 330                 335     


Val Ile Asn Glu Pro Pro Phe Leu Ile Glu Pro Leu Ile Tyr Leu Thr 
            340                 345                 350         


Asn Pro Lys Gly Ile Ile Gly Leu Ala Ser Ser Ser Leu Ile Tyr Thr 
        355                 360                 365             


Pro Leu Leu Ser Pro Ser Thr Gln Cys Leu Ser Ile Gly Glu Leu Ile 
    370                 375                 380                 


Ile Asn Leu Ile Gln Lys Tyr Ser Met Val Glu Asn Thr Glu Met Ile 
385                 390                 395                 400 


Gln Glu His Leu Glu Ile Ile Lys Lys Phe Asn Phe Ile Asn Ile Leu 
                405                 410                 415     


Asn Asp Leu Asn Gly Val Ile Ser Asn Pro Leu Phe Lys Thr Glu Glu 
            420                 425                 430         


Thr Phe Glu Thr Leu Leu Lys Ser Ala Glu Phe Ala Tyr Lys Ser Lys 
        435                 440                 445             


Asn Tyr Phe Gln Ala Ile Phe Tyr Trp Gln Leu Ala Ser Lys Asn Asn 
    450                 455                 460                 


Ile Thr Leu Leu Gly His Lys Ala Leu Trp Tyr Tyr Asn Ala Leu Tyr 
465                 470                 475                 480 


Asn Val Lys Gln Ile Tyr Lys Met Glu Tyr Ser Asp Ile Phe Tyr Ile 
                485                 490                 495     


Asp Asn Ile Ser Val Asp Phe His Ser Lys Asp Lys Leu Thr Trp Glu 
            500                 505                 510         


Lys Ile Lys His Tyr Tyr Tyr Ser Ala Asp Asn Arg Ile Gly Arg Asp 
        515                 520                 525             


Arg 
    


<210>  36
<211>  528
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ST8-PSTNmB

<400>  36

Met Arg Ser Ile Arg Lys Arg Trp Thr Ile Cys Thr Ile Ser Leu Leu 
1               5                   10                  15      


Leu Ile Phe Tyr Lys Thr Lys Glu Ile Ala Arg Thr Glu Glu His Gln 
            20                  25                  30          


Glu Thr Gln Leu Ile Gly Asp Gly Glu Leu Ser Leu Ser Arg Ser Leu 
        35                  40                  45              


Val Asn Ser Gly Ser Trp Leu Thr Thr Ser Pro Phe Tyr Leu Thr Pro 
    50                  55                  60                  


Pro Arg Asn Asn Leu Phe Val Ile Ser Asn Leu Gly Gln Leu Asn Gln 
65                  70                  75                  80  


Val Gln Ser Leu Ile Lys Ile Gln Lys Leu Thr Asn Asn Leu Leu Val 
                85                  90                  95      


Ile Leu Tyr Thr Ser Gln Asn Leu Lys Met Pro Lys Leu Val His Gln 
            100                 105                 110         


Ser Ala Asn Lys Asn Leu Phe Glu Ser Ile Tyr Leu Phe Glu Leu Pro 
        115                 120                 125             


Arg Ser Pro Asn Asn Ile Thr Pro Lys Lys Leu Leu Tyr Ile Tyr Arg 
    130                 135                 140                 


Ser Tyr Lys Lys Ile Leu Asn Ile Ile Gln Pro Ala His Leu Tyr Met 
145                 150                 155                 160 


Leu Ser Phe Thr Gly His Tyr Ser Tyr Leu Ile Ser Ile Ala Lys Lys 
                165                 170                 175     


Lys Asn Ile Thr Thr His Leu Ile Asp Glu Gly Thr Gly Thr Tyr Ala 
            180                 185                 190         


Pro Leu Leu Glu Ser Phe Ser Tyr His Pro Thr Lys Leu Glu Arg Tyr 
        195                 200                 205             


Leu Ile Gly Asn Asn Leu Asn Ile Lys Gly Tyr Ile Asp His Phe Asp 
    210                 215                 220                 


Ile Leu His Val Pro Phe Pro Glu Tyr Ala Lys Lys Ile Phe Asn Ala 
225                 230                 235                 240 


Lys Lys Tyr Asn Arg Phe Phe Ala His Ala Gly Gly Ile Ser Ile Asn 
                245                 250                 255     


Asn Asn Ile Ala Asn Leu Gln Lys Lys Tyr Gln Ile Ser Lys Asn Asp 
            260                 265                 270         


Tyr Ile Phe Val Ser Gln Arg Tyr Pro Ile Ser Asp Asp Leu Tyr Tyr 
        275                 280                 285             


Lys Ser Ile Val Glu Ile Leu Asn Ser Ile Ser Leu Gln Ile Lys Gly 
    290                 295                 300                 


Lys Ile Phe Ile Lys Leu His Pro Lys Glu Met Gly Asn Asn Tyr Val 
305                 310                 315                 320 


Met Ser Leu Phe Leu Asn Met Val Glu Ile Asn Pro Arg Leu Val Val 
                325                 330                 335     


Ile Asn Glu Pro Pro Phe Leu Ile Glu Pro Leu Ile Tyr Leu Thr Asn 
            340                 345                 350         


Pro Lys Gly Ile Ile Gly Leu Ala Ser Ser Ser Leu Ile Tyr Thr Pro 
        355                 360                 365             


Leu Leu Ser Pro Ser Thr Gln Cys Leu Ser Ile Gly Glu Leu Ile Ile 
    370                 375                 380                 


Asn Leu Ile Gln Lys Tyr Ser Met Val Glu Asn Thr Glu Met Ile Gln 
385                 390                 395                 400 


Glu His Leu Glu Ile Ile Lys Lys Phe Asn Phe Ile Asn Ile Leu Asn 
                405                 410                 415     


Asp Leu Asn Gly Val Ile Ser Asn Pro Leu Phe Lys Thr Glu Glu Thr 
            420                 425                 430         


Phe Glu Thr Leu Leu Lys Ser Ala Glu Phe Ala Tyr Lys Ser Lys Asn 
        435                 440                 445             


Tyr Phe Gln Ala Ile Phe Tyr Trp Gln Leu Ala Ser Lys Asn Asn Ile 
    450                 455                 460                 


Thr Leu Leu Gly His Lys Ala Leu Trp Tyr Tyr Asn Ala Leu Tyr Asn 
465                 470                 475                 480 


Val Lys Gln Ile Tyr Lys Met Glu Tyr Ser Asp Ile Phe Tyr Ile Asp 
                485                 490                 495     


Asn Ile Ser Val Asp Phe His Ser Lys Asp Lys Leu Thr Trp Glu Lys 
            500                 505                 510         


Ile Lys His Tyr Tyr Tyr Ser Ala Asp Asn Arg Ile Gly Arg Asp Arg 
        515                 520                 525             


<210>  37
<211>  314
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ST6-CstII

<400>  37

Met Ile His Thr Asn Leu Lys Lys Lys Phe Ser Leu Phe Ile Leu Val 
1               5                   10                  15      


Phe Leu Leu Phe Ala Val Ile Cys Val Trp Lys Lys Gly Ser Asp Tyr 
            20                  25                  30          


Glu Ala Leu Thr Leu Gln Ala Lys Glu Phe Gln Met Pro Lys Ser Gln 
        35                  40                  45              


Glu Lys Val Ala Leu Glu Lys Lys Val Ile Ile Ala Gly Asn Gly Pro 
    50                  55                  60                  


Ser Leu Lys Glu Ile Asp Tyr Ser Arg Leu Pro Asn Asp Phe Asp Val 
65                  70                  75                  80  


Phe Arg Cys Asn Gln Phe Tyr Phe Glu Asp Lys Tyr Tyr Leu Gly Lys 
                85                  90                  95      


Lys Cys Lys Ala Val Phe Tyr Asn Pro Ser Leu Phe Phe Glu Gln Tyr 
            100                 105                 110         


Tyr Thr Leu Lys His Leu Ile Gln Asn Gln Glu Tyr Glu Thr Glu Leu 
        115                 120                 125             


Ile Met Cys Ser Asn Tyr Asn Gln Ala His Leu Glu Asn Glu Asn Phe 
    130                 135                 140                 


Val Lys Thr Phe Tyr Asp Tyr Phe Pro Asp Ala His Leu Gly Tyr Asp 
145                 150                 155                 160 


Phe Phe Lys Gln Leu Lys Asp Phe Asn Ala Tyr Phe Lys Phe His Glu 
                165                 170                 175     


Ile Tyr Phe Asn Gln Arg Ile Thr Ser Gly Val Tyr Met Cys Ala Val 
            180                 185                 190         


Ala Ile Ala Leu Gly Tyr Lys Glu Ile Tyr Leu Ser Gly Ile Asp Phe 
        195                 200                 205             


Tyr Gln Asn Gly Ser Ser Tyr Ala Phe Asp Thr Lys Gln Lys Asn Leu 
    210                 215                 220                 


Leu Lys Leu Ala Pro Asn Phe Lys Asn Asp Asn Ser His Tyr Ile Gly 
225                 230                 235                 240 


His Ser Lys Asn Thr Asp Ile Lys Ala Leu Glu Phe Leu Glu Lys Thr 
                245                 250                 255     


Tyr Lys Ile Lys Leu Tyr Cys Leu Cys Pro Asn Ser Leu Leu Ala Asn 
            260                 265                 270         


Phe Ile Glu Leu Ala Pro Asn Leu Asn Ser Asn Phe Ile Ile Gln Glu 
        275                 280                 285             


Lys Asn Asn Tyr Thr Lys Asp Ile Leu Ile Pro Ser Ser Glu Ala Tyr 
    290                 295                 300                 


Gly Lys Phe Ser Lys Asn Ile Asn Phe Lys 
305                 310                 


<210>  38
<211>  313
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ST8-CstII

<400>  38

Met Arg Ser Ile Arg Lys Arg Trp Thr Ile Cys Thr Ile Ser Leu Leu 
1               5                   10                  15      


Leu Ile Phe Tyr Lys Thr Lys Glu Ile Ala Arg Thr Glu Glu His Gln 
            20                  25                  30          


Glu Thr Gln Leu Ile Gly Asp Gly Glu Leu Ser Leu Ser Arg Ser Leu 
        35                  40                  45              


Val Asn Ser Gly Ser Lys Lys Val Ile Ile Ala Gly Asn Gly Pro Ser 
    50                  55                  60                  


Leu Lys Glu Ile Asp Tyr Ser Arg Leu Pro Asn Asp Phe Asp Val Phe 
65                  70                  75                  80  


Arg Cys Asn Gln Phe Tyr Phe Glu Asp Lys Tyr Tyr Leu Gly Lys Lys 
                85                  90                  95      


Cys Lys Ala Val Phe Tyr Asn Pro Ser Leu Phe Phe Glu Gln Tyr Tyr 
            100                 105                 110         


Thr Leu Lys His Leu Ile Gln Asn Gln Glu Tyr Glu Thr Glu Leu Ile 
        115                 120                 125             


Met Cys Ser Asn Tyr Asn Gln Ala His Leu Glu Asn Glu Asn Phe Val 
    130                 135                 140                 


Lys Thr Phe Tyr Asp Tyr Phe Pro Asp Ala His Leu Gly Tyr Asp Phe 
145                 150                 155                 160 


Phe Lys Gln Leu Lys Asp Phe Asn Ala Tyr Phe Lys Phe His Glu Ile 
                165                 170                 175     


Tyr Phe Asn Gln Arg Ile Thr Ser Gly Val Tyr Met Cys Ala Val Ala 
            180                 185                 190         


Ile Ala Leu Gly Tyr Lys Glu Ile Tyr Leu Ser Gly Ile Asp Phe Tyr 
        195                 200                 205             


Gln Asn Gly Ser Ser Tyr Ala Phe Asp Thr Lys Gln Lys Asn Leu Leu 
    210                 215                 220                 


Lys Leu Ala Pro Asn Phe Lys Asn Asp Asn Ser His Tyr Ile Gly His 
225                 230                 235                 240 


Ser Lys Asn Thr Asp Ile Lys Ala Leu Glu Phe Leu Glu Lys Thr Tyr 
                245                 250                 255     


Lys Ile Lys Leu Tyr Cys Leu Cys Pro Asn Ser Leu Leu Ala Asn Phe 
            260                 265                 270         


Ile Glu Leu Ala Pro Asn Leu Asn Ser Asn Phe Ile Ile Gln Glu Lys 
        275                 280                 285             


Asn Asn Tyr Thr Lys Asp Ile Leu Ile Pro Ser Ser Glu Ala Tyr Gly 
    290                 295                 300                 


Lys Phe Ser Lys Asn Ile Asn Phe Lys 
305                 310             


