                         SEQUENCE LISTING

<110>  GlaxoSmithKline Intellectual Property Development Limited
 
<120>  ANTIBODIES WITH REDUCED BINDING TO PROCESS IMPURITIES

<130>  PB66137

<150>  US62/404849
<151>  2016-10-06

<160>  38    

<170>  PatentIn version 3.5

<210>  1
<211>  61
<212>  PRT
<213>  Human

<400>  1

Ser Ser Val Val Thr Val Pro Ser Ser Ser Leu Gly Thr Gln Thr Tyr 
1               5                   10                  15      


Ile Cys Asn Val Asn His Lys Pro Ser Asn Thr Lys Val Asp Lys Lys 
            20                  25                  30          


Val Glu Pro Lys Ser Cys Asp Lys Thr His Thr Cys Pro Pro Cys Pro 
        35                  40                  45              


Ala Pro Glu Leu Leu Gly Gly Pro Ser Val Phe Leu Phe 
    50                  55                  60      


<210>  2
<211>  61
<212>  PRT
<213>  Human

<400>  2

Ser Ser Val Val Thr Val Pro Ser Ser Ser Leu Gly Thr Gln Thr Tyr 
1               5                   10                  15      


Ile Cys Asn Val Asn His Lys Pro Ser Asn Thr Lys Val Asp Lys Lys 
            20                  25                  30          


Val Glu Pro Lys Ser Cys Asp Lys Thr His Thr Cys Pro Pro Cys Pro 
        35                  40                  45              


Ala Pro Glu Leu Leu Gly Gly Pro Ser Val Phe Leu Phe 
    50                  55                  60      


<210>  3
<211>  57
<212>  PRT
<213>  Artificial

<220>
<223>  mAb3 (IgG2)

<400>  3

Ser Ser Val Val Thr Val Pro Ser Ser Asn Phe Gly Thr Gln Thr Tyr 
1               5                   10                  15      


Thr Cys Asn Val Asp His Lys Pro Ser Asn Thr Lys Val Asp Lys Thr 
            20                  25                  30          


Val Glu Arg Lys Cys Cys Val Glu Cys Pro Pro Cys Pro Ala Pro Pro 
        35                  40                  45              


Ala Ala Ala Ser Ser Val Phe Leu Phe 
    50                  55          


<210>  4
<211>  57
<212>  PRT
<213>  Artificial

<220>
<223>  mAb4 (IgG2/4)

<400>  4

Ser Ser Val Val Thr Val Pro Ser Ser Asn Phe Gly Thr Gln Thr Tyr 
1               5                   10                  15      


Thr Cys Asn Val Asp His Lys Pro Ser Asn Thr Lys Val Asp Lys Thr 
            20                  25                  30          


Val Glu Arg Lys Cys Cys Val Glu Cys Pro Pro Cys Pro Ala Pro Pro 
        35                  40                  45              


Ala Ala Ala Ser Ser Val Phe Leu Phe 
    50                  55          


<210>  5
<211>  58
<212>  PRT
<213>  Artificial

<220>
<223>  mAb5 (IgG4)

<400>  5

Ser Ser Val Val Thr Val Pro Ser Ser Ser Leu Gly Thr Lys Thr Tyr 
1               5                   10                  15      


Thr Cys Asn Val Asp His Lys Pro Ser Asn Thr Lys Val Asp Lys Arg 
            20                  25                  30          


Val Glu Ser Lys Tyr Gly Pro Pro Cys Pro Pro Cys Pro Ala Pro Glu 
        35                  40                  45              


Phe Glu Gly Gly Pro Ser Val Phe Leu Phe 
    50                  55              


<210>  6
<211>  57
<212>  PRT
<213>  Artificial

<220>
<223>  mAb6  (IgG4)

<400>  6

Ser Ser Val Val Thr Val Pro Ser Ser Ser Leu Gly Thr Lys Thr Tyr 
1               5                   10                  15      


Thr Cys Asn Val Asp His Lys Pro Ser Asn Thr Lys Val Asp Lys Arg 
            20                  25                  30          


Val Glu Ser Lys Tyr Gly Pro Pro Cys Pro Pro Cys Pro Ala Pro Pro 
        35                  40                  45              


Ala Ala Ala Pro Ser Val Phe Leu Phe 
    50                  55          


<210>  7
<211>  58
<212>  PRT
<213>  Artificial

<220>
<223>  mAb7 (IgG4)

<400>  7

Ser Ser Val Val Thr Val Pro Ser Ser Ser Leu Gly Thr Lys Thr Tyr 
1               5                   10                  15      


Thr Cys Asn Val Asp His Lys Pro Ser Asn Thr Lys Val Asp Lys Arg 
            20                  25                  30          


Val Glu Ser Lys Tyr Gly Pro Pro Cys Pro Pro Cys Pro Ala Pro Glu 
        35                  40                  45              


Phe Glu Gly Gly Pro Ser Val Phe Leu Phe 
    50                  55              


<210>  8
<211>  61
<212>  PRT
<213>  Human

<400>  8

Ser Ser Val Val Thr Val Pro Ser Ser Ser Leu Gly Thr Gln Thr Tyr 
1               5                   10                  15      


Ile Cys Asn Val Asn His Lys Pro Ser Asn Thr Lys Val Asp Lys Lys 
            20                  25                  30          


Val Glu Pro Lys Ser Cys Asp Lys Thr His Thr Cys Pro Pro Cys Pro 
        35                  40                  45              


Ala Pro Glu Leu Leu Gly Gly Pro Ser Val Phe Leu Phe 
    50                  55                  60      


<210>  9
<211>  57
<212>  PRT
<213>  Human

<400>  9

Ser Ser Val Val Thr Val Pro Ser Ser Asn Phe Gly Thr Gln Thr Tyr 
1               5                   10                  15      


Thr Cys Asn Val Asp His Lys Pro Ser Asn Thr Lys Val Asp Lys Thr 
            20                  25                  30          


Val Glu Arg Lys Cys Cys Val Glu Cys Pro Pro Cys Pro Ala Pro Pro 
        35                  40                  45              


Val Ala Gly Pro Ser Val Phe Leu Phe 
    50                  55          


<210>  10
<211>  58
<212>  PRT
<213>  Human

<400>  10

Ser Ser Val Val Thr Val Pro Ser Ser Ser Leu Gly Thr Lys Thr Tyr 
1               5                   10                  15      


Thr Cys Asn Val Asp His Lys Pro Ser Asn Thr Lys Val Asp Lys Arg 
            20                  25                  30          


Val Glu Ser Lys Tyr Gly Pro Pro Cys Pro Ser Cys Pro Ala Pro Glu 
        35                  40                  45              


Phe Leu Gly Gly Pro Ser Val Phe Leu Phe 
    50                  55              


<210>  11
<211>  58
<212>  PRT
<213>  Artificial

<220>
<223>  mAb5-1B (IgG4)

<400>  11

Ser Ser Val Val Thr Val Pro Ser Ser Ser Leu Gly Thr Gln Thr Tyr 
1               5                   10                  15      


Thr Cys Asn Val Asp His Lys Pro Ser Asn Thr Lys Val Asp Lys Arg 
            20                  25                  30          


Val Glu Ser Lys Tyr Gly Pro Pro Cys Pro Pro Cys Pro Ala Pro Glu 
        35                  40                  45              


Phe Glu Gly Gly Pro Ser Val Phe Leu Phe 
    50                  55              


<210>  12
<211>  58
<212>  PRT
<213>  Artificial

<220>
<223>  mAb5-2B (IgG4)

<400>  12

Ser Ser Val Val Thr Val Pro Ser Ser Ser Leu Gly Thr Lys Thr Tyr 
1               5                   10                  15      


Thr Cys Asn Val Asp His Lys Pro Ser Asn Thr Lys Val Asp Lys Lys 
            20                  25                  30          


Val Glu Ser Lys Tyr Gly Pro Pro Cys Pro Pro Cys Pro Ala Pro Glu 
        35                  40                  45              


Phe Glu Gly Gly Pro Ser Val Phe Leu Phe 
    50                  55              


<210>  13
<211>  58
<212>  PRT
<213>  Artificial

<220>
<223>  mAb5-3B (IgG4)

<400>  13

Ser Ser Val Val Thr Val Pro Ser Ser Ser Leu Gly Thr Lys Thr Tyr 
1               5                   10                  15      


Thr Cys Asn Val Asp His Lys Pro Ser Asn Thr Lys Val Asp Lys Arg 
            20                  25                  30          


Val Glu Pro Lys Tyr Gly Pro Pro Cys Pro Pro Cys Pro Ala Pro Glu 
        35                  40                  45              


Phe Glu Gly Gly Pro Ser Val Phe Leu Phe 
    50                  55              


<210>  14
<211>  58
<212>  PRT
<213>  Artificial

<220>
<223>  mAb5-4B (IgG4)

<400>  14

Ser Ser Val Val Thr Val Pro Ser Ser Ser Leu Gly Thr Lys Thr Tyr 
1               5                   10                  15      


Thr Cys Asn Val Asp His Lys Pro Ser Asn Thr Lys Val Asp Lys Lys 
            20                  25                  30          


Val Glu Pro Lys Tyr Gly Pro Pro Cys Pro Pro Cys Pro Ala Pro Glu 
        35                  40                  45              


Phe Glu Gly Gly Pro Ser Val Phe Leu Phe 
    50                  55              


<210>  15
<211>  58
<212>  PRT
<213>  Artificial

<220>
<223>  mAb5-5B (IgG4)

<400>  15

Ser Ser Val Val Thr Val Pro Ser Ser Ser Leu Gly Thr Lys Thr Tyr 
1               5                   10                  15      


Thr Cys Asn Val Asp His Lys Pro Ser Asn Thr Lys Val Asp Lys Arg 
            20                  25                  30          


Val Glu Ser Lys Cys Cys Val Glu Cys Pro Pro Cys Pro Ala Pro Glu 
        35                  40                  45              


Phe Glu Gly Gly Pro Ser Val Phe Leu Phe 
    50                  55              


<210>  16
<211>  61
<212>  PRT
<213>  Artificial

<220>
<223>  mAb5-6B (IgG4)

<400>  16

Ser Ser Val Val Thr Val Pro Ser Ser Ser Leu Gly Thr Lys Thr Tyr 
1               5                   10                  15      


Thr Cys Asn Val Asp His Lys Pro Ser Asn Thr Lys Val Asp Lys Arg 
            20                  25                  30          


Val Glu Ser Lys Ser Cys Asp Lys Thr His Thr Cys Pro Pro Cys Pro 
        35                  40                  45              


Ala Pro Glu Phe Glu Gly Gly Pro Ser Val Phe Leu Phe 
    50                  55                  60      


<210>  17
<211>  58
<212>  PRT
<213>  Artificial

<220>
<223>  mAb5-7B (IgG4)

<400>  17

Ser Ser Val Val Thr Val Pro Ser Ser Ser Leu Gly Thr Lys Thr Tyr 
1               5                   10                  15      


Thr Cys Asn Val Asp His Lys Pro Ser Asn Thr Lys Val Asp Lys Arg 
            20                  25                  30          


Val Leu Ser Lys Tyr Gly Pro Pro Cys Pro Pro Cys Pro Ala Pro Glu 
        35                  40                  45              


Phe Glu Gly Gly Pro Ser Val Phe Leu Phe 
    50                  55              


<210>  18
<211>  61
<212>  PRT
<213>  Human

<400>  18

Ser Ser Val Val Thr Val Pro Ser Ser Ser Leu Gly Thr Gln Thr Tyr 
1               5                   10                  15      


Ile Cys Asn Val Asn His Lys Pro Ser Asn Thr Lys Val Asp Lys Lys 
            20                  25                  30          


Val Glu Pro Lys Ser Cys Asp Lys Thr His Thr Cys Pro Pro Cys Pro 
        35                  40                  45              


Ala Pro Glu Leu Leu Gly Gly Pro Ser Val Phe Leu Phe 
    50                  55                  60      


<210>  19
<211>  4
<212>  PRT
<213>  Human

<400>  19

Cys Pro Ser Cys 
1               


<210>  20
<211>  4
<212>  PRT
<213>  Human

<400>  20

Cys Pro Pro Cys 
1               


<210>  21
<211>  12
<212>  PRT
<213>  Human

<400>  21

Glu Ser Lys Tyr Gly Pro Pro Cys Pro Ser Cys Pro 
1               5                   10          


<210>  22
<211>  12
<212>  PRT
<213>  Artificial

<220>
<223>  Unmodified IgG4 antibody hinge (S_P)

<400>  22

Glu Ser Lys Tyr Gly Pro Pro Cys Pro Pro Cys Pro 
1               5                   10          


<210>  23
<211>  4
<212>  PRT
<213>  Human

<400>  23

Tyr Gly Pro Pro 
1               


<210>  24
<211>  7
<212>  PRT
<213>  Human

<400>  24

Ser Cys Asp Lys Thr His Thr 
1               5           


<210>  25
<211>  4
<212>  PRT
<213>  Human

<400>  25

Cys Cys Val Glu 
1               


<210>  26
<211>  7
<212>  PRT
<213>  Human

<400>  26

Glu Ser Lys Tyr Gly Pro Pro 
1               5           


<210>  27
<211>  10
<212>  PRT
<213>  Human

<400>  27

Glu Pro Lys Ser Cys Asp Lys Thr His Thr 
1               5                   10  


<210>  28
<211>  7
<212>  PRT
<213>  Artificial

<220>
<223>  Modification of IgG4 Kabat residues 226 to 238

<400>  28

Glu Arg Lys Tyr Gly Pro Pro 
1               5           


<210>  29
<211>  7
<212>  PRT
<213>  Human

<400>  29

Glu Arg Lys Cys Cys Val Glu 
1               5           


<210>  30
<211>  12
<212>  PRT
<213>  Artificial

<220>
<223>  Modification of IgG4 Kabat residues 226 to 238

<400>  30

Glu Leu Lys Thr Pro Leu Gly Asp Thr Thr His Thr 
1               5                   10          


<210>  31
<211>  6
<212>  PRT
<213>  Human

<400>  31

Glu Phe Leu Gly Gly Pro 
1               5       


<210>  32
<211>  5
<212>  PRT
<213>  Artificial

<220>
<223>  Modification of IgG4 Kabat residues 246 to 251

<400>  32

Pro Ala Ala Ala Ser 
1               5   


<210>  33
<211>  5
<212>  PRT
<213>  Artificial

<220>
<223>  Modification of IgG4 Kabat residues 246 to 251

<400>  33

Pro Ala Ala Ala Pro 
1               5   


<210>  34
<211>  61
<212>  PRT
<213>  Artificial

<220>
<223>  mAb2-alphaB(IgG1)

<400>  34

Ser Ser Val Val Thr Val Pro Ser Ser Ser Leu Gly Thr Gln Thr Tyr 
1               5                   10                  15      


Ile Cys Asn Val Asn His Lys Pro Ser Asn Thr Lys Val Asp Lys Arg 
            20                  25                  30          


Val Glu Pro Lys Ser Cys Asp Lys Thr His Thr Cys Pro Pro Cys Pro 
        35                  40                  45              


Ala Pro Glu Leu Leu Gly Gly Pro Ser Val Phe Leu Phe 
    50                  55                  60      


<210>  35
<211>  58
<212>  PRT
<213>  Artificial

<220>
<223>  mAb5-8B(IgG4)

<400>  35

Ser Ser Val Val Thr Val Pro Ser Ser Ser Leu Gly Thr Lys Thr Tyr 
1               5                   10                  15      


Thr Cys Asn Val Asp His Lys Pro Ser Asn Thr Lys Val Asp Lys Thr 
            20                  25                  30          


Val Glu Ser Lys Tyr Gly Pro Pro Cys Pro Pro Cys Pro Ala Pro Glu 
        35                  40                  45              


Phe Glu Gly Gly Pro Ser Val Phe Leu Phe 
    50                  55              


<210>  36
<211>  58
<212>  PRT
<213>  Artificial

<220>
<223>  mAb7-2B(IgG4)

<400>  36

Ser Ser Val Val Thr Val Pro Ser Ser Ser Leu Gly Thr Lys Thr Tyr 
1               5                   10                  15      


Thr Cys Asn Val Asp His Lys Pro Ser Asn Thr Lys Val Asp Lys Lys 
            20                  25                  30          


Val Glu Ser Lys Tyr Gly Pro Pro Cys Pro Pro Cys Pro Ala Pro Glu 
        35                  40                  45              


Phe Glu Gly Gly Pro Ser Val Phe Leu Phe 
    50                  55              


<210>  37
<211>  58
<212>  PRT
<213>  Artificial

<220>
<223>  mAb7-4B (IgG4)

<400>  37

Ser Ser Val Val Thr Val Pro Ser Ser Ser Leu Gly Thr Lys Thr Tyr 
1               5                   10                  15      


Thr Cys Asn Val Asp His Lys Pro Ser Asn Thr Lys Val Asp Lys Lys 
            20                  25                  30          


Val Glu Pro Lys Tyr Gly Pro Pro Cys Pro Pro Cys Pro Ala Pro Glu 
        35                  40                  45              


Phe Glu Gly Gly Pro Ser Val Phe Leu Phe 
    50                  55              


<210>  38
<211>  58
<212>  PRT
<213>  Artificial

<220>
<223>  mAb7-5B(IgG4)

<400>  38

Ser Ser Val Val Thr Val Pro Ser Ser Ser Leu Gly Thr Lys Thr Tyr 
1               5                   10                  15      


Thr Cys Asn Val Asp His Lys Pro Ser Asn Thr Lys Val Asp Lys Arg 
            20                  25                  30          


Val Glu Ser Lys Cys Cys Val Glu Cys Pro Pro Cys Pro Ala Pro Glu 
        35                  40                  45              


Phe Glu Gly Gly Pro Ser Val Phe Leu Phe 
    50                  55              


