                         SEQUENCE LISTING

<110>  Precision NanoSystems Inc.
 
<120>  Compositions for Transfecting Resistant Cell Types

<130>  PNI-IP-014PC

<150>  62/403,640
<151>  2016-10-03

<160>  6     

<170>  PatentIn version 3.5

<210>  1
<211>  966
<212>  DNA
<213>  Artificial

<220>
<223>  mRNA eGFP ORF


<220>
<221>  misc_feature
<222>  (721)..(846)
<223>  n is a, c, g, t or u

<400>  1
atggtgagca agggcgagga gctgttcacc ggggtggtgc ccatcctggt cgagctggac       60

ggcgacgtaa acggccacaa gttcagcgtg tccggcgagg gcgagggcga tgccacctac      120

ggcaagctga ccctgaagtt catctgcacc accggcaagc tgcccgtgcc ctggcccacc      180

ctcgtgacca ccctgaccta cggcgtgcag tgcttcagcc gctaccccga ccacatgaag      240

cagcacgact tcttcaagtc cgccatgccc gaaggctacg tccaggagcg caccatcttc      300

ttcaaggacg acggcaacta caagacccgc gccgaggtga agttcgaggg cgacaccctg      360

gtgaaccgca tcgagctgaa gggcatcgac ttcaaggagg acggcaacat cctggggcac      420

aagctggagt acaactacaa cagccacaac gtctatatca tggccgacaa gcagaagaac      480

ggcatcaagg tgaacttcaa gatccgccac aacatcgagg acggcagcgt gcagctcgcc      540

gaccactacc agcagaacac ccccatcggc gacggccccg tgctgctgcc cgacaaccac      600

tacctgagca cccagtccgc cctgagcaaa gaccccaacg agaagcgcga tcacatggtc      660

ctgctggagt tcgtgaccgc cgccgggatc actctcggca tggacgagct gtacaagtaa      720

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn      780

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn      840

nnnnnnaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa      900

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa      960

aaaaaa                                                                 966


<210>  2
<211>  25
<212>  DNA
<213>  Artificial

<220>
<223>  HPRT siRNA first strand

<400>  2
gccagacuuu guuggauuug aaatt                                             25


<210>  3
<211>  26
<212>  DNA
<213>  Artificial

<220>
<223>  HPRT siRNA second strand

<400>  3
aauuucaauc caacaaaguc uggcuu                                            26


<210>  4
<211>  23
<212>  DNA
<213>  Artificial

<220>
<223>  Sense strand for DsiNC-1 control

<400>  4
cguuaaucgc guauaauacg cgu                                               23


<210>  5
<211>  27
<212>  DNA
<213>  Artificial

<220>
<223>  Antisense strand for DsiNC

<400>  5
auacgcguau uauacgcgau uaacgac                                           27


<210>  6
<211>  5514
<212>  DNA
<213>  Artificial

<220>
<223>  Circular dsDNA pCX-EGFP

<400>  6
gtcgacattg attattgact agttattaat agtaatcaat tacggggtca ttagttcata       60

gcccatatat ggagttccgc gttacataac ttacggtaaa tggcccgcct ggctgaccgc      120

ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt tcccatagta acgccaatag      180

ggactttcca ttgacgtcaa tgggtggact atttacggta aactgcccac ttggcagtac      240

atcaagtgta tcatatgcca agtacgcccc ctattgacgt caatgacggt aaatggcccg      300

cctggcatta tgcccagtac atgaccttat gggactttcc tacttggcag tacatctacg      360

tattagtcat cgctattacc atgggtcgag gtgagcccca cgttctgctt cactctcccc      420

atctcccccc cctccccacc cccaattttg tatttattta ttttttaatt attttgtgca      480

gcgatggggg cggggggggg gggggcgcgc gccaggcggg gcggggcggg gcgaggggcg      540

gggcggggcg aggcggagag gtgcggcggc agccaatcag agcggcgcgc tccgaaagtt      600

tccttttatg gcgaggcggc ggcggcggcg gccctataaa aagcgaagcg cgcggcgggc      660

gggagtcgct gcgttgcctt cgccccgtgc cccgctccgc gccgcctcgc gccgcccgcc      720

ccggctctga ctgaccgcgt tactcccaca ggtgagcggg cgggacggcc cttctcctcc      780

gggctgtaat tagcgcttgg tttaatgacg gctcgtttct tttctgtggc tgcgtgaaag      840

ccttaaaggg ctccgggagg gccctttgtg cgggggggag cggctcgggg ggtgcgtgcg      900

tgtgtgtgtg cgtggggagc gccgcgtgcg gcccgcgctg cccggcggct gtgagcgctg      960

cgggcgcggc gcggggcttt gtgcgctccg cgtgtgcgcg aggggagcgc ggccgggggc     1020

ggtgccccgc ggtgcggggg ggctgcgagg ggaacaaagg ctgcgtgcgg ggtgtgtgcg     1080

tgggggggtg agcagggggt gtgggcgcgg cggtcgggct gtaacccccc cctgcacccc     1140

cctccccgag ttgctgagca cggcccggct tcgggtgcgg ggctccgtac ggggcgtggc     1200

gcggggctcg ccgtgccggg cggggggtgg cggcaggtgg gggtgccggg cggggcgggg     1260

ccgcctcggg ccggggaggg ctcgggggag gggcgcggcg gcccccggag cgccggcggc     1320

tgtcgaggcg cggcgagccg cagccattgc cttttatggt aatcgtgcga gagggcgcag     1380

ggacttcctt tgtcccaaat ctgtgcggag ccgaaatctg ggaggcgccg ccgcaccccc     1440

tctagcgggc gcggggcgaa gcggtgcggc gccggcagga aggaactggg cggggagggc     1500

cttcgtgcgt cgccgcgccg ccgtcccctt ctccctctcc agcctcgggg ctgtccgcgg     1560

ggggacggct gccttcgggg gggacggggc agggcggggt tcggcttctg gcgtgtgacc     1620

ggcggctcta gagcctctgc taaccatgtt catgccttct tctttttcct acagctcctg     1680

ggcaacgtgc tggttattgt gctgtctcat cattttggca aagaattcgc caccatggtg     1740

agcaagggcg aggagctgtt caccggggtg gtgcccatcc tggtcgagct ggacggcgac     1800

gtgaacggcc acaagttcag cgtgtccggc gagggcgagg gcgatgccac ctacggcaag     1860

ctgaccctga agttcatctg caccaccggc aagctgcccg tgccctggcc caccctcgtg     1920

accaccctga cctacggcgt gcagtgcttc agccgctacc ccgaccacat gaagcagcac     1980

gacttcttca agtccgccat gcccgaaggc tacgtccagg agcgcaccat cttcttcaag     2040

gacgacggca actacaagac ccgcgccgag gtgaagttcg agggcgacac cctggtgaac     2100

cgcatcgagc tgaagggcat cgacttcaag gaggacggca acatcctggg gcacaagctg     2160

gagtacaact acaacagcca caacgtctat atcatggccg acaagcagaa gaacggcatc     2220

aaggtgaact tcaagatccg ccacaacatc gaggacggca gcgtgcagct cgccgaccac     2280

taccagcaga acacccccat cggcgacggc cccgtgctgc tgcccgacaa ccactacctg     2340

agcacccagt ccgccctgag caaagacccc aacgagaagc gcgatcacat ggtcctgctg     2400

gagttcgtga ccgccgccgg gatcactcac ggcatggacg agctgtacaa gtaagaattc     2460

actcctcagg tgcaggctgc ctatcagaag gtggtggctg gtgtggccaa tgccctggct     2520

cacaaatacc actgagatct ttttccctct gccaaaaatt atggggacat catgaagccc     2580

cttgagcatc tgacttctgg ctaataaagg aaatttattt tcattgcaat agtgtgttgg     2640

aattttttgt gtctctcact cggaaggaca tatgggaggg caaatcattt aaaacatcag     2700

aatgagtatt tggtttagag tttggcaaca tatgccatat gctggctgcc atgaacaaag     2760

gtggctataa agaggtcatc agtatatgaa acagccccct gctgtccatt ccttattcca     2820

tagaaaagcc ttgacttgag gttagatttt ttttatattt tgttttgtgt tatttttttc     2880

tttaacatcc ctaaaatttt ccttacatgt tttactagcc agatttttcc tcctctcctg     2940

actactccca gtcatagctg tccctcttct cttatgaaga tccctcgacc tgcagcccaa     3000

gcttggcgta atcatggtca tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc     3060

cacacaacat acgagccgga agcataaagt gtaaagcctg gggtgcctaa tgagtgagct     3120

aactcacatt aattgcgttg cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc     3180

agcggatccg catctcaatt agtcagcaac catagtcccg cccctaactc cgcccatccc     3240

gcccctaact ccgcccagtt ccgcccattc tccgccccat ggctgactaa ttttttttat     3300

ttatgcagag gccgaggccg cctcggcctc tgagctattc cagaagtagt gaggaggctt     3360

ttttggaggc ctaggctttt gcaaaaagct aacttgttta ttgcagctta taatggttac     3420

aaataaagca atagcatcac aaatttcaca aataaagcat ttttttcact gcattctagt     3480

tgtggtttgt ccaaactcat caatgtatct tatcatgtct ggatccgctg cattaatgaa     3540

tcggccaacg cgcggggaga ggcggtttgc gtattgggcg ctcttccgct tcctcgctca     3600

ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg     3660

taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc     3720

agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc     3780

cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac     3840

tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc     3900

tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcaat     3960

gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc     4020

acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca     4080

acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag     4140

cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta     4200

gaaggacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg     4260

gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc     4320

agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt     4380

ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa     4440

ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat     4500

atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct atctcagcga     4560

tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata actacgatac     4620

gggagggctt accatctggc cccagtgctg caatgatacc gcgagaccca cgctcaccgg     4680

ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga agtggtcctg     4740

caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga gtaagtagtt     4800

cgccagttaa tagtttgcgc aacgttgttg ccattgctac aggcatcgtg gtgtcacgct     4860

cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga gttacatgat     4920

cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt gtcagaagta     4980

agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct cttactgtca     5040

tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca ttctgagaat     5100

agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat acgggataat accgcgccac     5160

atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga aaactctcaa     5220

ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc aactgatctt     5280

cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg     5340

caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc ctttttcaat     5400

attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt     5460

agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca cctg           5514


