                         SEQUENCE LISTING

<110>  Poseida Therapeutics
       OSTERTAG, Eric
       SHEDLOCK, Devon
 
<120>  MODIFIED STEM CELL MEMORY T CELLS, METHODS OF MAKING AND METHODS 
       OF USING SAME

<130>  POTH-012/01WO

<150>  62/402,707
<151>  2016-09-30

<150>  62/553,058
<151>  2017-08-31

<150>  62/556,309
<151>  2017-09-08

<150>  62/502,508
<151>  2017-05-05

<160>  42    

<170>  PatentIn version 3.5

<210>  1
<211>  89
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  FN3 domain consensus sequence

<400>  1

Leu Pro Ala Pro Lys Asn Leu Val Val Ser Glu Val Thr Glu Asp Ser 
1               5                   10                  15      


Leu Arg Leu Ser Trp Thr Ala Pro Asp Ala Ala Phe Asp Ser Phe Leu 
            20                  25                  30          


Ile Gln Tyr Gln Glu Ser Glu Lys Val Gly Glu Ala Ile Asn Leu Thr 
        35                  40                  45              


Val Pro Gly Ser Glu Arg Ser Tyr Asp Leu Thr Gly Leu Lys Pro Gly 
    50                  55                  60                  


Thr Glu Tyr Thr Val Ser Ile Tyr Gly Val Lys Gly Gly His Arg Ser 
65                  70                  75                  80  


Asn Pro Leu Ser Ala Glu Phe Thr Thr 
                85                  


<210>  2
<211>  90
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  FN3 consensus sequence

<400>  2

Met Leu Pro Ala Pro Lys Asn Leu Val Val Ser Glu Val Thr Glu Asp 
1               5                   10                  15      


Ser Leu Arg Leu Ser Trp Thr Ala Pro Asp Ala Ala Phe Asp Ser Phe 
            20                  25                  30          


Leu Ile Gln Tyr Gln Glu Ser Glu Lys Val Gly Glu Ala Ile Asn Leu 
        35                  40                  45              


Thr Val Pro Gly Ser Glu Arg Ser Tyr Asp Leu Thr Gly Leu Lys Pro 
    50                  55                  60                  


Gly Thr Glu Tyr Thr Val Ser Ile Tyr Gly Val Lys Gly Gly His Arg 
65                  70                  75                  80  


Ser Asn Pro Leu Ser Ala Glu Phe Thr Thr 
                85                  90  


<210>  3
<211>  270
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  FN3 consensus sequence

<400>  3
atgctgcctg caccaaagaa cctggtggtg tctcatgtga cagaggatag tgccagactg       60

tcatggactg ctcccgacgc agccttcgat agttttatca tcgtgtaccg ggagaacatc      120

gaaaccggcg aggccattgt cctgacagtg ccagggtccg aacgctctta tgacctgaca      180

gatctgaagc ccggaactga gtactatgtg cagatcgccg gcgtcaaagg aggcaatatc      240

agcttccctc tgtccgcaat cttcaccaca                                       270


<210>  4
<211>  594
<212>  PRT
<213>  Trichoplusia ni

<400>  4

Met Gly Ser Ser Leu Asp Asp Glu His Ile Leu Ser Ala Leu Leu Gln 
1               5                   10                  15      


Ser Asp Asp Glu Leu Val Gly Glu Asp Ser Asp Ser Glu Ile Ser Asp 
            20                  25                  30          


His Val Ser Glu Asp Asp Val Gln Ser Asp Thr Glu Glu Ala Phe Ile 
        35                  40                  45              


Asp Glu Val His Glu Val Gln Pro Thr Ser Ser Gly Ser Glu Ile Leu 
    50                  55                  60                  


Asp Glu Gln Asn Val Ile Glu Gln Pro Gly Ser Ser Leu Ala Ser Asn 
65                  70                  75                  80  


Arg Ile Leu Thr Leu Pro Gln Arg Thr Ile Arg Gly Lys Asn Lys His 
                85                  90                  95      


Cys Trp Ser Thr Ser Lys Ser Thr Arg Arg Ser Arg Val Ser Ala Leu 
            100                 105                 110         


Asn Ile Val Arg Ser Gln Arg Gly Pro Thr Arg Met Cys Arg Asn Ile 
        115                 120                 125             


Tyr Asp Pro Leu Leu Cys Phe Lys Leu Phe Phe Thr Asp Glu Ile Ile 
    130                 135                 140                 


Ser Glu Ile Val Lys Trp Thr Asn Ala Glu Ile Ser Leu Lys Arg Arg 
145                 150                 155                 160 


Glu Ser Met Thr Gly Ala Thr Phe Arg Asp Thr Asn Glu Asp Glu Ile 
                165                 170                 175     


Tyr Ala Phe Phe Gly Ile Leu Val Met Thr Ala Val Arg Lys Asp Asn 
            180                 185                 190         


His Met Ser Thr Asp Asp Leu Phe Asp Arg Ser Leu Ser Met Val Tyr 
        195                 200                 205             


Val Ser Val Met Ser Arg Asp Arg Phe Asp Phe Leu Ile Arg Cys Leu 
    210                 215                 220                 


Arg Met Asp Asp Lys Ser Ile Arg Pro Thr Leu Arg Glu Asn Asp Val 
225                 230                 235                 240 


Phe Thr Pro Val Arg Lys Ile Trp Asp Leu Phe Ile His Gln Cys Ile 
                245                 250                 255     


Gln Asn Tyr Thr Pro Gly Ala His Leu Thr Ile Asp Glu Gln Leu Leu 
            260                 265                 270         


Gly Phe Arg Gly Arg Cys Pro Phe Arg Met Tyr Ile Pro Asn Lys Pro 
        275                 280                 285             


Ser Lys Tyr Gly Ile Lys Ile Leu Met Met Cys Asp Ser Gly Tyr Lys 
    290                 295                 300                 


Tyr Met Ile Asn Gly Met Pro Tyr Leu Gly Arg Gly Thr Gln Thr Asn 
305                 310                 315                 320 


Gly Val Pro Leu Gly Glu Tyr Tyr Val Lys Glu Leu Ser Lys Pro Val 
                325                 330                 335     


His Gly Ser Cys Arg Asn Ile Thr Cys Asp Asn Trp Phe Thr Ser Ile 
            340                 345                 350         


Pro Leu Ala Lys Asn Leu Leu Gln Glu Pro Tyr Lys Leu Thr Ile Val 
        355                 360                 365             


Gly Thr Val Arg Ser Asn Lys Arg Glu Ile Pro Glu Val Leu Lys Asn 
    370                 375                 380                 


Ser Arg Ser Arg Pro Val Gly Thr Ser Met Phe Cys Phe Asp Gly Pro 
385                 390                 395                 400 


Leu Thr Leu Val Ser Tyr Lys Pro Lys Pro Ala Lys Met Val Tyr Leu 
                405                 410                 415     


Leu Ser Ser Cys Asp Glu Asp Ala Ser Ile Asn Glu Ser Thr Gly Lys 
            420                 425                 430         


Pro Gln Met Val Met Tyr Tyr Asn Gln Thr Lys Gly Gly Val Asp Thr 
        435                 440                 445             


Leu Asp Gln Met Cys Ser Val Met Thr Cys Ser Arg Lys Thr Asn Arg 
    450                 455                 460                 


Trp Pro Met Ala Leu Leu Tyr Gly Met Ile Asn Ile Ala Cys Ile Asn 
465                 470                 475                 480 


Ser Phe Ile Ile Tyr Ser His Asn Val Ser Ser Lys Gly Glu Lys Val 
                485                 490                 495     


Gln Ser Arg Lys Lys Phe Met Arg Asn Leu Tyr Met Ser Leu Thr Ser 
            500                 505                 510         


Ser Phe Met Arg Lys Arg Leu Glu Ala Pro Thr Leu Lys Arg Tyr Leu 
        515                 520                 525             


Arg Asp Asn Ile Ser Asn Ile Leu Pro Asn Glu Val Pro Gly Thr Ser 
    530                 535                 540                 


Asp Asp Ser Thr Glu Glu Pro Val Met Lys Lys Arg Thr Tyr Cys Thr 
545                 550                 555                 560 


Tyr Cys Pro Ser Lys Ile Arg Arg Lys Ala Asn Ala Ser Cys Lys Lys 
                565                 570                 575     


Cys Lys Lys Val Ile Cys Arg Glu His Asn Ile Asp Met Cys Gln Ser 
            580                 585                 590         


Cys Phe 
        


<210>  5
<211>  594
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Super Piggybac Transposase

<400>  5

Met Gly Ser Ser Leu Asp Asp Glu His Ile Leu Ser Ala Leu Leu Gln 
1               5                   10                  15      


Ser Asp Asp Glu Leu Val Gly Glu Asp Ser Asp Ser Glu Val Ser Asp 
            20                  25                  30          


His Val Ser Glu Asp Asp Val Gln Ser Asp Thr Glu Glu Ala Phe Ile 
        35                  40                  45              


Asp Glu Val His Glu Val Gln Pro Thr Ser Ser Gly Ser Glu Ile Leu 
    50                  55                  60                  


Asp Glu Gln Asn Val Ile Glu Gln Pro Gly Ser Ser Leu Ala Ser Asn 
65                  70                  75                  80  


Arg Ile Leu Thr Leu Pro Gln Arg Thr Ile Arg Gly Lys Asn Lys His 
                85                  90                  95      


Cys Trp Ser Thr Ser Lys Ser Thr Arg Arg Ser Arg Val Ser Ala Leu 
            100                 105                 110         


Asn Ile Val Arg Ser Gln Arg Gly Pro Thr Arg Met Cys Arg Asn Ile 
        115                 120                 125             


Tyr Asp Pro Leu Leu Cys Phe Lys Leu Phe Phe Thr Asp Glu Ile Ile 
    130                 135                 140                 


Ser Glu Ile Val Lys Trp Thr Asn Ala Glu Ile Ser Leu Lys Arg Arg 
145                 150                 155                 160 


Glu Ser Met Thr Ser Ala Thr Phe Arg Asp Thr Asn Glu Asp Glu Ile 
                165                 170                 175     


Tyr Ala Phe Phe Gly Ile Leu Val Met Thr Ala Val Arg Lys Asp Asn 
            180                 185                 190         


His Met Ser Thr Asp Asp Leu Phe Asp Arg Ser Leu Ser Met Val Tyr 
        195                 200                 205             


Val Ser Val Met Ser Arg Asp Arg Phe Asp Phe Leu Ile Arg Cys Leu 
    210                 215                 220                 


Arg Met Asp Asp Lys Ser Ile Arg Pro Thr Leu Arg Glu Asn Asp Val 
225                 230                 235                 240 


Phe Thr Pro Val Arg Lys Ile Trp Asp Leu Phe Ile His Gln Cys Ile 
                245                 250                 255     


Gln Asn Tyr Thr Pro Gly Ala His Leu Thr Ile Asp Glu Gln Leu Leu 
            260                 265                 270         


Gly Phe Arg Gly Arg Cys Pro Phe Arg Val Tyr Ile Pro Asn Lys Pro 
        275                 280                 285             


Ser Lys Tyr Gly Ile Lys Ile Leu Met Met Cys Asp Ser Gly Thr Lys 
    290                 295                 300                 


Tyr Met Ile Asn Gly Met Pro Tyr Leu Gly Arg Gly Thr Gln Thr Asn 
305                 310                 315                 320 


Gly Val Pro Leu Gly Glu Tyr Tyr Val Lys Glu Leu Ser Lys Pro Val 
                325                 330                 335     


His Gly Ser Cys Arg Asn Ile Thr Cys Asp Asn Trp Phe Thr Ser Ile 
            340                 345                 350         


Pro Leu Ala Lys Asn Leu Leu Gln Glu Pro Tyr Lys Leu Thr Ile Val 
        355                 360                 365             


Gly Thr Val Arg Ser Asn Lys Arg Glu Ile Pro Glu Val Leu Lys Asn 
    370                 375                 380                 


Ser Arg Ser Arg Pro Val Gly Thr Ser Met Phe Cys Phe Asp Gly Pro 
385                 390                 395                 400 


Leu Thr Leu Val Ser Tyr Lys Pro Lys Pro Ala Lys Met Val Tyr Leu 
                405                 410                 415     


Leu Ser Ser Cys Asp Glu Asp Ala Ser Ile Asn Glu Ser Thr Gly Lys 
            420                 425                 430         


Pro Gln Met Val Met Tyr Tyr Asn Gln Thr Lys Gly Gly Val Asp Thr 
        435                 440                 445             


Leu Asp Gln Met Cys Ser Val Met Thr Cys Ser Arg Lys Thr Asn Arg 
    450                 455                 460                 


Trp Pro Met Ala Leu Leu Tyr Gly Met Ile Asn Ile Ala Cys Ile Asn 
465                 470                 475                 480 


Ser Phe Ile Ile Tyr Ser His Asn Val Ser Ser Lys Gly Glu Lys Val 
                485                 490                 495     


Gln Ser Arg Lys Lys Phe Met Arg Asn Leu Tyr Met Ser Leu Thr Ser 
            500                 505                 510         


Ser Phe Met Arg Lys Arg Leu Glu Ala Pro Thr Leu Lys Arg Tyr Leu 
        515                 520                 525             


Arg Asp Asn Ile Ser Asn Ile Leu Pro Lys Glu Val Pro Gly Thr Ser 
    530                 535                 540                 


Asp Asp Ser Thr Glu Glu Pro Val Met Lys Lys Arg Thr Tyr Cys Thr 
545                 550                 555                 560 


Tyr Cys Pro Ser Lys Ile Arg Arg Lys Ala Asn Ala Ser Cys Lys Lys 
                565                 570                 575     


Cys Lys Lys Val Ile Cys Arg Glu His Asn Ile Asp Met Cys Gln Ser 
            580                 585                 590         


Cys Phe 
        


<210>  6
<211>  340
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Sleeping Beauty Transposase

<400>  6

Met Gly Lys Ser Lys Glu Ile Ser Gln Asp Leu Arg Lys Lys Ile Val 
1               5                   10                  15      


Asp Leu His Lys Ser Gly Ser Ser Leu Gly Ala Ile Ser Lys Arg Leu 
            20                  25                  30          


Lys Val Pro Arg Ser Ser Val Gln Thr Ile Val Arg Lys Tyr Lys His 
        35                  40                  45              


His Gly Thr Thr Gln Pro Ser Tyr Arg Ser Gly Arg Arg Arg Tyr Leu 
    50                  55                  60                  


Ser Pro Arg Asp Glu Arg Thr Leu Val Arg Lys Val Gln Ile Asn Pro 
65                  70                  75                  80  


Arg Thr Thr Ala Lys Asp Leu Val Lys Met Leu Glu Glu Thr Gly Thr 
                85                  90                  95      


Lys Val Ser Ile Ser Thr Val Lys Arg Val Leu Tyr Arg His Asn Leu 
            100                 105                 110         


Lys Gly Arg Ser Ala Arg Lys Lys Pro Leu Leu Gln Asn Arg His Lys 
        115                 120                 125             


Lys Ala Arg Leu Arg Phe Ala Thr Ala His Gly Asp Lys Asp Arg Thr 
    130                 135                 140                 


Phe Trp Arg Asn Val Leu Trp Ser Asp Glu Thr Lys Ile Glu Leu Phe 
145                 150                 155                 160 


Gly His Asn Asp His Arg Tyr Val Trp Arg Lys Lys Gly Glu Ala Cys 
                165                 170                 175     


Lys Pro Lys Asn Thr Ile Pro Thr Val Lys His Gly Gly Gly Ser Ile 
            180                 185                 190         


Met Leu Trp Gly Cys Phe Ala Ala Gly Gly Thr Gly Ala Leu His Lys 
        195                 200                 205             


Ile Asp Gly Ile Met Arg Lys Glu Asn Tyr Val Asp Ile Leu Lys Gln 
    210                 215                 220                 


His Leu Lys Thr Ser Val Arg Lys Leu Lys Leu Gly Arg Lys Trp Val 
225                 230                 235                 240 


Phe Gln Met Asp Asn Asp Pro Lys His Thr Ser Lys Val Val Ala Lys 
                245                 250                 255     


Trp Leu Lys Asp Asn Lys Val Lys Val Leu Glu Trp Pro Ser Gln Ser 
            260                 265                 270         


Pro Asp Leu Asn Pro Ile Glu Asn Leu Trp Ala Glu Leu Lys Lys Arg 
        275                 280                 285             


Val Arg Ala Arg Arg Pro Thr Asn Leu Thr Gln Leu His Gln Leu Cys 
    290                 295                 300                 


Gln Glu Glu Trp Ala Lys Ile His Pro Thr Tyr Cys Gly Lys Leu Val 
305                 310                 315                 320 


Glu Gly Tyr Pro Lys Arg Leu Thr Gln Val Lys Gln Phe Lys Gly Asn 
                325                 330                 335     


Ala Thr Lys Tyr 
            340 


<210>  7
<211>  340
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  hyperactive sleeping beauty transposase

<400>  7

Met Gly Lys Ser Lys Glu Ile Ser Gln Asp Leu Arg Lys Arg Ile Val 
1               5                   10                  15      


Asp Leu His Lys Ser Gly Ser Ser Leu Gly Ala Ile Ser Lys Arg Leu 
            20                  25                  30          


Ala Val Pro Arg Ser Ser Val Gln Thr Ile Val Arg Lys Tyr Lys His 
        35                  40                  45              


His Gly Thr Thr Gln Pro Ser Tyr Arg Ser Gly Arg Arg Arg Tyr Leu 
    50                  55                  60                  


Ser Pro Arg Asp Glu Arg Thr Leu Val Arg Lys Val Gln Ile Asn Pro 
65                  70                  75                  80  


Arg Thr Thr Ala Lys Asp Leu Val Lys Met Leu Glu Glu Thr Gly Thr 
                85                  90                  95      


Lys Val Ser Ile Ser Thr Val Lys Arg Val Leu Tyr Arg His Asn Leu 
            100                 105                 110         


Lys Gly His Ser Ala Arg Lys Lys Pro Leu Leu Gln Asn Arg His Lys 
        115                 120                 125             


Lys Ala Arg Leu Arg Phe Ala Thr Ala His Gly Asp Lys Asp Arg Thr 
    130                 135                 140                 


Phe Trp Arg Asn Val Leu Trp Ser Asp Glu Thr Lys Ile Glu Leu Phe 
145                 150                 155                 160 


Gly His Asn Asp His Arg Tyr Val Trp Arg Lys Lys Gly Glu Ala Cys 
                165                 170                 175     


Lys Pro Lys Asn Thr Ile Pro Thr Val Lys His Gly Gly Gly Ser Ile 
            180                 185                 190         


Met Leu Trp Gly Cys Phe Ala Ala Gly Gly Thr Gly Ala Leu His Lys 
        195                 200                 205             


Ile Asp Gly Ile Met Asp Ala Val Gln Tyr Val Asp Ile Leu Lys Gln 
    210                 215                 220                 


His Leu Lys Thr Ser Val Arg Lys Leu Lys Leu Gly Arg Lys Trp Val 
225                 230                 235                 240 


Phe Gln His Asp Asn Asp Pro Lys His Thr Ser Lys Val Val Ala Lys 
                245                 250                 255     


Trp Leu Lys Asp Asn Lys Val Lys Val Leu Glu Trp Pro Ser Gln Ser 
            260                 265                 270         


Pro Asp Leu Asn Pro Ile Glu Asn Leu Trp Ala Glu Leu Lys Lys Arg 
        275                 280                 285             


Val Arg Ala Arg Arg Pro Thr Asn Leu Thr Gln Leu His Gln Leu Cys 
    290                 295                 300                 


Gln Glu Glu Trp Ala Lys Ile His Pro Asn Tyr Cys Gly Lys Leu Val 
305                 310                 315                 320 


Glu Gly Tyr Pro Lys Arg Leu Thr Gln Val Lys Gln Phe Lys Gly Asn 
                325                 330                 335     


Ala Thr Lys Tyr 
            340 


<210>  8
<211>  21
<212>  PRT
<213>  Homo sapiens

<400>  8

Met Ala Leu Pro Val Thr Ala Leu Leu Leu Pro Leu Ala Leu Leu Leu 
1               5                   10                  15      


His Ala Ala Arg Pro 
            20      


<210>  9
<211>  63
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  nucleotide sequence for human CD8alpha signal peptide

<400>  9
atggcactgc cagtcaccgc cctgctgctg cctctggctc tgctgctgca cgcagctaga       60

cca                                                                     63


<210>  10
<211>  24
<212>  PRT
<213>  Homo sapiens

<400>  10

Ile Tyr Ile Trp Ala Pro Leu Ala Gly Thr Cys Gly Val Leu Leu Leu 
1               5                   10                  15      


Ser Leu Val Ile Thr Leu Tyr Cys 
            20                  


<210>  11
<211>  72
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  nucleotide sequence coding for human CD8alpha transmembrane 
       domain

<400>  11
atctacattt gggcaccact ggccgggacc tgtggagtgc tgctgctgag cctggtcatc       60

acactgtact gc                                                           72


<210>  12
<211>  112
<212>  PRT
<213>  Homo sapiens

<400>  12

Arg Val Lys Phe Ser Arg Ser Ala Asp Ala Pro Ala Tyr Lys Gln Gly 
1               5                   10                  15      


Gln Asn Gln Leu Tyr Asn Glu Leu Asn Leu Gly Arg Arg Glu Glu Tyr 
            20                  25                  30          


Asp Val Leu Asp Lys Arg Arg Gly Arg Asp Pro Glu Met Gly Gly Lys 
        35                  40                  45              


Pro Arg Arg Lys Asn Pro Gln Glu Gly Leu Tyr Asn Glu Leu Gln Lys 
    50                  55                  60                  


Asp Lys Met Ala Glu Ala Tyr Ser Glu Ile Gly Met Lys Gly Glu Arg 
65                  70                  75                  80  


Arg Arg Gly Lys Gly His Asp Gly Leu Tyr Gln Gly Leu Ser Thr Ala 
                85                  90                  95      


Thr Lys Asp Thr Tyr Asp Ala Leu His Met Gln Ala Leu Pro Pro Arg 
            100                 105                 110         


<210>  13
<211>  336
<212>  DNA
<213>  Artificial Sequenec

<220>
<223>  nucleotide sequence encoding the CD28 costimulatory domain

<400>  13
cgcgtgaagt ttagtcgatc agcagatgcc ccagcttaca aacagggaca gaaccagctg       60

tataacgagc tgaatctggg ccgccgagag gaatatgacg tgctggataa gcggagagga      120

cgcgaccccg aaatgggagg caagcccagg cgcaaaaacc ctcaggaagg cctgtataac      180

gagctgcaga aggacaaaat ggcagaagcc tattctgaga tcggcatgaa gggggagcga      240

cggagaggca aagggcacga tgggctgtac cagggactga gcaccgccac aaaggacacc      300

tatgatgctc tgcatatgca ggcactgcct ccaagg                                336


<210>  14
<211>  42
<212>  PRT
<213>  Homo sapiens

<400>  14

Lys Arg Gly Arg Lys Lys Leu Leu Tyr Ile Phe Lys Gln Pro Phe Met 
1               5                   10                  15      


Arg Pro Val Gln Thr Thr Gln Glu Glu Asp Gly Cys Ser Cys Arg Phe 
            20                  25                  30          


Pro Glu Glu Glu Glu Gly Gly Cys Glu Leu 
        35                  40          


<210>  15
<211>  126
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  nucleotide sequence encoding the 4-1BB costimulatory domain

<400>  15
aagagaggca ggaagaaact gctgtatatt ttcaaacagc ccttcatgcg ccccgtgcag       60

actacccagg aggaagacgg gtgctcctgt cgattccctg aggaagagga aggcgggtgt      120

gagctg                                                                 126


<210>  16
<211>  45
<212>  PRT
<213>  Homo sapiens

<400>  16

Thr Thr Thr Pro Ala Pro Arg Pro Pro Thr Pro Ala Pro Thr Ile Ala 
1               5                   10                  15      


Ser Gln Pro Leu Ser Leu Arg Pro Glu Ala Cys Arg Pro Ala Ala Gly 
            20                  25                  30          


Gly Ala Val His Thr Arg Gly Leu Asp Phe Ala Cys Asp 
        35                  40                  45  


<210>  17
<211>  135
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  nucleotide sequence encoding human CD8alpha hinge

<400>  17
actaccacac cagcacctag accaccaact ccagctccaa ccatcgcgag tcagcccctg       60

agtctgagac ctgaggcctg caggccagct gcaggaggag ctgtgcacac caggggcctg      120

gacttcgcct gcgac                                                       135


<210>  18
<211>  18
<212>  PRT
<213>  Thosea asigna

<400>  18

Glu Gly Arg Gly Ser Leu Leu Thr Cys Gly Asp Val Glu Glu Asn Pro 
1               5                   10                  15      


Gly Pro 
        


<210>  19
<211>  21
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  GSG-T2A

<400>  19

Gly Ser Gly Glu Gly Arg Gly Ser Leu Leu Thr Cys Gly Asp Val Glu 
1               5                   10                  15      


Glu Asn Pro Gly Pro 
            20      


<210>  20
<211>  63
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  GSG-T2A

<400>  20
ggatctggag agggaagggg aagcctgctg acctgtggag acgtggagga aaacccagga       60

cca                                                                     63


<210>  21
<211>  20
<212>  PRT
<213>  Equine rhinitis A

<400>  21

Gln Cys Thr Asn Tyr Ala Leu Leu Lys Leu Ala Gly Asp Val Glu Ser 
1               5                   10                  15      


Asn Pro Gly Pro 
            20  


<210>  22
<211>  23
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  GSG-E2A peptide

<400>  22

Gly Ser Gly Gln Cys Thr Asn Tyr Ala Leu Leu Lys Leu Ala Gly Asp 
1               5                   10                  15      


Val Glu Ser Asn Pro Gly Pro 
            20              


<210>  23
<211>  22
<212>  PRT
<213>  Foot and nout disease virus type O

<400>  23

Val Lys Gln Thr Leu Asn Phe Asp Leu Leu Lys Leu Ala Gly Asp Val 
1               5                   10                  15      


Glu Ser Asn Pro Gly Pro 
            20          


<210>  24
<211>  25
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  GSG-F2A peptide

<400>  24

Gly Ser Gly Val Lys Gln Thr Leu Asn Phe Asp Leu Leu Lys Leu Ala 
1               5                   10                  15      


Gly Asp Val Glu Ser Asn Pro Gly Pro 
            20                  25  


<210>  25
<211>  19
<212>  PRT
<213>  Porcine teschovirus-1

<400>  25

Ala Thr Asn Phe Ser Leu Leu Lys Gln Ala Gly Asp Val Glu Glu Asn 
1               5                   10                  15      


Pro Gly Pro 
            


<210>  26
<211>  22
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  GSG-P2A peptide

<400>  26

Gly Ser Gly Ala Thr Asn Phe Ser Leu Leu Lys Gln Ala Gly Asp Val 
1               5                   10                  15      


Glu Glu Asn Pro Gly Pro 
            20          


<210>  27
<211>  5296
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Helraiser transposon

<400>  27
tcctatataa taaaagagaa acatgcaaat tgaccatccc tccgctacgc tcaagccacg       60

cccaccagcc aatcagaagt gactatgcaa attaacccaa caaagatggc agttaaattt      120

gcatacgcag gtgtcaagcg ccccaggagg caacggcggc cgcgggctcc caggaccttc      180

gctggccccg ggaggcgagg ccggccgcgc ctagccacac ccgcgggctc ccgggacctt      240

cgccagcaga gagcagagcg ggagagcggg cggagagcgg gaggtttgga ggacttggca      300

gagcaggagg ccgctggaca tagagcagag cgagagagag ggtggcttgg agggcgtggc      360

tccctctgtc accccagctt cctcatcaca gctgtggaaa ctgacagcag ggaggaggaa      420

gtcccacccc cacagaatca gccagaatca gccgttggtc agacagctct cagcggcctg      480

acagccagga ctctcattca cctgcatctc agaccgtgac agtagagagg tgggactatg      540

tctaaagaac aactgttgat acaacgtagc tctgcagccg aaagatgccg gcgttatcga      600

cagaaaatgt ctgcagagca acgtgcgtct gatcttgaaa gaaggcggcg cctgcaacag      660

aatgtatctg aagagcagct actggaaaaa cgtcgctctg aagccgaaaa acagcggcgt      720

catcgacaga aaatgtctaa agaccaacgt gcctttgaag ttgaaagaag gcggtggcga      780

cgacagaata tgtctagaga acagtcatca acaagtacta ccaataccgg taggaactgc      840

cttctcagca aaaatggagt acatgaggat gcaattctcg aacatagttg tggtggaatg      900

actgttcgat gtgaattttg cctatcacta aatttctctg atgaaaaacc atccgatggg      960

aaatttactc gatgttgtag caaagggaaa gtctgtccaa atgatataca ttttccagat     1020

tacccggcat atttaaaaag attaatgaca aacgaagatt ctgacagtaa aaatttcatg     1080

gaaaatattc gttccataaa tagttctttt gcttttgctt ccatgggtgc aaatattgca     1140

tcgccatcag gatatgggcc atactgtttt agaatacacg gacaagttta tcaccgtact     1200

ggaactttac atccttcgga tggtgtttct cggaagtttg ctcaactcta tattttggat     1260

acagccgaag ctacaagtaa aagattagca atgccagaaa accagggctg ctcagaaaga     1320

ctcatgatca acatcaacaa cctcatgcat gaaataaatg aattaacaaa atcgtacaag     1380

atgctacatg aggtagaaaa ggaagcccaa tctgaagcag cagcaaaagg tattgctccc     1440

acagaagtaa caatggcgat taaatacgat cgtaacagtg acccaggtag atataattct     1500

ccccgtgtaa ccgaggttgc tgtcatattc agaaacgaag atggagaacc tccttttgaa     1560

agggacttgc tcattcattg taaaccagat cccaataatc caaatgccac taaaatgaaa     1620

caaatcagta tcctgtttcc tacattagat gcaatgacat atcctattct ttttccacat     1680

ggtgaaaaag gctggggaac agatattgca ttaagactca gagacaacag tgtaatcgac     1740

aataatacta gacaaaatgt aaggacacga gtcacacaaa tgcagtatta tggatttcat     1800

ctctctgtgc gggacacgtt caatcctatt ttaaatgcag gaaaattaac tcaacagttt     1860

attgtggatt catattcaaa aatggaggcc aatcggataa atttcatcaa agcaaaccaa     1920

tctaagttga gagttgaaaa atatagtggt ttgatggatt atctcaaatc tagatctgaa     1980

aatgacaatg tgccgattgg taaaatgata atacttccat catcttttga gggtagtccc     2040

agaaatatgc agcagcgata tcaggatgct atggcaattg taacgaagta tggcaagccc     2100

gatttattca taaccatgac atgcaacccc aaatgggcag atattacaaa caatttacaa     2160

cgctggcaaa aagttgaaaa cagacctgac ttggtagcca gagtttttaa tattaagctg     2220

aatgctcttt taaatgatat atgtaaattc catttatttg gcaaagtaat agctaaaatt     2280

catgtcattg aatttcagaa acgcggactg cctcacgctc acatattatt gatattagat     2340

agtgagtcca aattacgttc agaagatgac attgaccgta tagttaaggc agaaattcca     2400

gatgaagacc agtgtcctcg actttttcaa attgtaaaat caaatatggt acatggacca     2460

tgtggaatac aaaatccaaa tagtccatgt atggaaaatg gaaaatgttc aaagggatat     2520

ccaaaagaat ttcaaaatgc gaccattgga aatattgatg gatatcccaa atacaaacga     2580

agatctggta gcaccatgtc tattggaaat aaagttgtcg ataacacttg gattgtccct     2640

tataacccgt atttgtgcct taaatataac tgtcatataa atgttgaagt ctgtgcatca     2700

attaaaagtg tcaaatattt atttaaatac atctataaag ggcacgattg tgcaaatatt     2760

caaatttctg aaaaaaatat tatcaatcat gacgaagtac aggacttcat tgactccagg     2820

tatgtgagcg ctcctgaggc tgtttggaga ctttttgcaa tgcgaatgca tgaccaatct     2880

catgcaatca caagattagc tattcatttg ccaaatgatc agaatttgta ttttcatacc     2940

gatgattttg ctgaagtttt agatagggct aaaaggcata actcgacttt gatggcttgg     3000

ttcttattga atagagaaga ttctgatgca cgtaattatt attattggga gattccacag     3060

cattatgtgt ttaataattc tttgtggaca aaacgccgaa agggtgggaa taaagtatta     3120

ggtagactgt tcactgtgag ctttagagaa ccagaacgat attaccttag acttttgctt     3180

ctgcatgtaa aaggtgcgat aagttttgag gatctgcgaa ctgtaggagg tgtaacttat     3240

gatacatttc atgaagctgc taaacaccga ggattattac ttgatgacac tatctggaaa     3300

gatacgattg acgatgcaat catccttaat atgcccaaac aactacggca actttttgca     3360

tatatatgtg tgtttggatg tccttctgct gcagacaaat tatgggatga gaataaatct     3420

cattttattg aagatttctg ttggaaatta caccgaagag aaggtgcctg tgtgaactgt     3480

gaaatgcatg cccttaacga aattcaggag gtattcacat tgcatggaat gaaatgttca     3540

catttcaaac ttccggacta tcctttatta atgaatgcaa atacatgtga tcaattgtac     3600

gagcaacaac aggcagaggt tttgataaat tctctgaatg atgaacagtt ggcagccttt     3660

cagactataa cttcagccat cgaagatcaa actgtacacc ccaaatgctt tttcttggat     3720

ggtccaggtg gtagtggaaa aacatatctg tataaagttt taacacatta tattagaggt     3780

cgtggtggta ctgttttacc cacagcatct acaggaattg ctgcaaattt acttcttggt     3840

ggaagaacct ttcattccca atataaatta ccaattccat taaatgaaac ttcaatttct     3900

agactcgata taaagagtga agttgctaaa accattaaaa aggcccaact tctcattatt     3960

gatgaatgca ccatggcatc cagtcatgct ataaacgcca tagatagatt actaagagaa     4020

attatgaatt tgaatgttgc atttggtggg aaagttctcc ttctcggagg ggattttcga     4080

caatgtctca gtattgtacc acatgctatg cgatcggcca tagtacaaac gagtttaaag     4140

tactgtaatg tttggggatg tttcagaaag ttgtctctta aaacaaatat gagatcagag     4200

gattctgctt atagtgaatg gttagtaaaa cttggagatg gcaaacttga tagcagtttt     4260

catttaggaa tggatattat tgaaatcccc catgaaatga tttgtaacgg atctattatt     4320

gaagctacct ttggaaatag tatatctata gataatatta aaaatatatc taaacgtgca     4380

attctttgtc caaaaaatga gcatgttcaa aaattaaatg aagaaatttt ggatatactt     4440

gatggagatt ttcacacata tttgagtgat gattccattg attcaacaga tgatgctgaa     4500

aaggaaaatt ttcccatcga atttcttaat agtattactc cttcgggaat gccgtgtcat     4560

aaattaaaat tgaaagtggg tgcaatcatc atgctattga gaaatcttaa tagtaaatgg     4620

ggtctttgta atggtactag atttattatc aaaagattac gacctaacat tatcgaagct     4680

gaagtattaa caggatctgc agagggagag gttgttctga ttccaagaat tgatttgtcc     4740

ccatctgaca ctggcctccc atttaaatta attcgaagac agtttcccgt gatgccagca     4800

tttgcgatga ctattaataa atcacaagga caaactctag acagagtagg aatattccta     4860

cctgaacccg ttttcgcaca tggtcagtta tatgttgctt tctctcgagt tcgaagagca     4920

tgtgacgtta aagttaaagt tgtaaatact tcatcacaag ggaaattagt caagcactct     4980

gaaagtgttt ttactcttaa tgtggtatac agggagatat tagaataagt ttaatcactt     5040

tatcagtcat tgtttgcatc aatgttgttt ttatatcatg tttttgttgt ttttatatca     5100

tgtctttgtt gttgttatat catgttgtta ttgtttattt attaataaat ttatgtatta     5160

ttttcatata cattttactc atttcctttc atctctcaca cttctattat agagaaaggg     5220

caaatagcaa tattaaaata tttcctctaa ttaattccct ttcaatgtgc acgaatttcg     5280

tgcaccgggc cactag                                                     5296


<210>  28
<211>  1496
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Helitron transposase

<400>  28

Met Ser Lys Glu Gln Leu Leu Ile Gln Arg Ser Ser Ala Ala Glu Arg 
1               5                   10                  15      


Cys Arg Arg Tyr Arg Gln Lys Met Ser Ala Glu Gln Arg Ala Ser Asp 
            20                  25                  30          


Leu Glu Arg Arg Arg Arg Leu Gln Gln Asn Val Ser Glu Glu Gln Leu 
        35                  40                  45              


Leu Glu Lys Arg Arg Ser Glu Ala Glu Lys Gln Arg Arg His Arg Gln 
    50                  55                  60                  


Lys Met Ser Lys Asp Gln Arg Ala Phe Glu Val Glu Arg Arg Arg Trp 
65                  70                  75                  80  


Arg Arg Gln Asn Met Ser Arg Glu Gln Ser Ser Thr Ser Thr Thr Asn 
                85                  90                  95      


Thr Gly Arg Asn Cys Leu Leu Ser Lys Asn Gly Val His Glu Asp Ala 
            100                 105                 110         


Ile Leu Glu His Ser Cys Gly Gly Met Thr Val Arg Cys Glu Phe Cys 
        115                 120                 125             


Leu Ser Leu Asn Phe Ser Asp Glu Lys Pro Ser Asp Gly Lys Phe Thr 
    130                 135                 140                 


Arg Cys Cys Ser Lys Gly Lys Val Cys Pro Asn Asp Ile His Phe Pro 
145                 150                 155                 160 


Asp Tyr Pro Ala Tyr Leu Lys Arg Leu Met Thr Asn Glu Asp Ser Asp 
                165                 170                 175     


Ser Lys Asn Phe Met Glu Asn Ile Arg Ser Ile Asn Ser Ser Phe Ala 
            180                 185                 190         


Phe Ala Ser Met Gly Ala Asn Ile Ala Ser Pro Ser Gly Tyr Gly Pro 
        195                 200                 205             


Tyr Cys Phe Arg Ile His Gly Gln Val Tyr His Arg Thr Gly Thr Leu 
    210                 215                 220                 


His Pro Ser Asp Gly Val Ser Arg Lys Phe Ala Gln Leu Tyr Ile Leu 
225                 230                 235                 240 


Asp Thr Ala Glu Ala Thr Ser Lys Arg Leu Ala Met Pro Glu Asn Gln 
                245                 250                 255     


Gly Cys Ser Glu Arg Leu Met Ile Asn Ile Asn Asn Leu Met His Glu 
            260                 265                 270         


Ile Asn Glu Leu Thr Lys Ser Tyr Lys Met Leu His Glu Val Glu Lys 
        275                 280                 285             


Glu Ala Gln Ser Glu Ala Ala Ala Lys Gly Ile Ala Pro Thr Glu Val 
    290                 295                 300                 


Thr Met Ala Ile Lys Tyr Asp Arg Asn Ser Asp Pro Gly Arg Tyr Asn 
305                 310                 315                 320 


Ser Pro Arg Val Thr Glu Val Ala Val Ile Phe Arg Asn Glu Asp Gly 
                325                 330                 335     


Glu Pro Pro Phe Glu Arg Asp Leu Leu Ile His Cys Lys Pro Asp Pro 
            340                 345                 350         


Asn Asn Pro Asn Ala Thr Lys Met Lys Gln Ile Ser Ile Leu Phe Pro 
        355                 360                 365             


Thr Leu Asp Ala Met Thr Tyr Pro Ile Leu Phe Pro His Gly Glu Lys 
    370                 375                 380                 


Gly Trp Gly Thr Asp Ile Ala Leu Arg Leu Arg Asp Asn Ser Val Ile 
385                 390                 395                 400 


Asp Asn Asn Thr Arg Gln Asn Val Arg Thr Arg Val Thr Gln Met Gln 
                405                 410                 415     


Tyr Tyr Gly Phe His Leu Ser Val Arg Asp Thr Phe Asn Pro Ile Leu 
            420                 425                 430         


Asn Ala Gly Lys Leu Thr Gln Gln Phe Ile Val Asp Ser Tyr Ser Lys 
        435                 440                 445             


Met Glu Ala Asn Arg Ile Asn Phe Ile Lys Ala Asn Gln Ser Lys Leu 
    450                 455                 460                 


Arg Val Glu Lys Tyr Ser Gly Leu Met Asp Tyr Leu Lys Ser Arg Ser 
465                 470                 475                 480 


Glu Asn Asp Asn Val Pro Ile Gly Lys Met Ile Ile Leu Pro Ser Ser 
                485                 490                 495     


Phe Glu Gly Ser Pro Arg Asn Met Gln Gln Arg Tyr Gln Asp Ala Met 
            500                 505                 510         


Ala Ile Val Thr Lys Tyr Gly Lys Pro Asp Leu Phe Ile Thr Met Thr 
        515                 520                 525             


Cys Asn Pro Lys Trp Ala Asp Ile Thr Asn Asn Leu Gln Arg Trp Gln 
    530                 535                 540                 


Lys Val Glu Asn Arg Pro Asp Leu Val Ala Arg Val Phe Asn Ile Lys 
545                 550                 555                 560 


Leu Asn Ala Leu Leu Asn Asp Ile Cys Lys Phe His Leu Phe Gly Lys 
                565                 570                 575     


Val Ile Ala Lys Ile His Val Ile Glu Phe Gln Lys Arg Gly Leu Pro 
            580                 585                 590         


His Ala His Ile Leu Leu Ile Leu Asp Ser Glu Ser Lys Leu Arg Ser 
        595                 600                 605             


Glu Asp Asp Ile Asp Arg Ile Val Lys Ala Glu Ile Pro Asp Glu Asp 
    610                 615                 620                 


Gln Cys Pro Arg Leu Phe Gln Ile Val Lys Ser Asn Met Val His Gly 
625                 630                 635                 640 


Pro Cys Gly Ile Gln Asn Pro Asn Ser Pro Cys Met Glu Asn Gly Lys 
                645                 650                 655     


Cys Ser Lys Gly Tyr Pro Lys Glu Phe Gln Asn Ala Thr Ile Gly Asn 
            660                 665                 670         


Ile Asp Gly Tyr Pro Lys Tyr Lys Arg Arg Ser Gly Ser Thr Met Ser 
        675                 680                 685             


Ile Gly Asn Lys Val Val Asp Asn Thr Trp Ile Val Pro Tyr Asn Pro 
    690                 695                 700                 


Tyr Leu Cys Leu Lys Tyr Asn Cys His Ile Asn Val Glu Val Cys Ala 
705                 710                 715                 720 


Ser Ile Lys Ser Val Lys Tyr Leu Phe Lys Tyr Ile Tyr Lys Gly His 
                725                 730                 735     


Asp Cys Ala Asn Ile Gln Ile Ser Glu Lys Asn Ile Ile Asn His Asp 
            740                 745                 750         


Glu Val Gln Asp Phe Ile Asp Ser Arg Tyr Val Ser Ala Pro Glu Ala 
        755                 760                 765             


Val Trp Arg Leu Phe Ala Met Arg Met His Asp Gln Ser His Ala Ile 
    770                 775                 780                 


Thr Arg Leu Ala Ile His Leu Pro Asn Asp Gln Asn Leu Tyr Phe His 
785                 790                 795                 800 


Thr Asp Asp Phe Ala Glu Val Leu Asp Arg Ala Lys Arg His Asn Ser 
                805                 810                 815     


Thr Leu Met Ala Trp Phe Leu Leu Asn Arg Glu Asp Ser Asp Ala Arg 
            820                 825                 830         


Asn Tyr Tyr Tyr Trp Glu Ile Pro Gln His Tyr Val Phe Asn Asn Ser 
        835                 840                 845             


Leu Trp Thr Lys Arg Arg Lys Gly Gly Asn Lys Val Leu Gly Arg Leu 
    850                 855                 860                 


Phe Thr Val Ser Phe Arg Glu Pro Glu Arg Tyr Tyr Leu Arg Leu Leu 
865                 870                 875                 880 


Leu Leu His Val Lys Gly Ala Ile Ser Phe Glu Asp Leu Arg Thr Val 
                885                 890                 895     


Gly Gly Val Thr Tyr Asp Thr Phe His Glu Ala Ala Lys His Arg Gly 
            900                 905                 910         


Leu Leu Leu Asp Asp Thr Ile Trp Lys Asp Thr Ile Asp Asp Ala Ile 
        915                 920                 925             


Ile Leu Asn Met Pro Lys Gln Leu Arg Gln Leu Phe Ala Tyr Ile Cys 
    930                 935                 940                 


Val Phe Gly Cys Pro Ser Ala Ala Asp Lys Leu Trp Asp Glu Asn Lys 
945                 950                 955                 960 


Ser His Phe Ile Glu Asp Phe Cys Trp Lys Leu His Arg Arg Glu Gly 
                965                 970                 975     


Ala Cys Val Asn Cys Glu Met His Ala Leu Asn Glu Ile Gln Glu Val 
            980                 985                 990         


Phe Thr Leu His Gly Met Lys Cys  Ser His Phe Lys Leu  Pro Asp Tyr 
        995                 1000                 1005             


Pro Leu  Leu Met Asn Ala Asn  Thr Cys Asp Gln Leu  Tyr Glu Gln 
    1010                 1015                 1020             


Gln Gln  Ala Glu Val Leu Ile  Asn Ser Leu Asn Asp  Glu Gln Leu 
    1025                 1030                 1035             


Ala Ala  Phe Gln Thr Ile Thr  Ser Ala Ile Glu Asp  Gln Thr Val 
    1040                 1045                 1050             


His Pro  Lys Cys Phe Phe Leu  Asp Gly Pro Gly Gly  Ser Gly Lys 
    1055                 1060                 1065             


Thr Tyr  Leu Tyr Lys Val Leu  Thr His Tyr Ile Arg  Gly Arg Gly 
    1070                 1075                 1080             


Gly Thr  Val Leu Pro Thr Ala  Ser Thr Gly Ile Ala  Ala Asn Leu 
    1085                 1090                 1095             


Leu Leu  Gly Gly Arg Thr Phe  His Ser Gln Tyr Lys  Leu Pro Ile 
    1100                 1105                 1110             


Pro Leu  Asn Glu Thr Ser Ile  Ser Arg Leu Asp Ile  Lys Ser Glu 
    1115                 1120                 1125             


Val Ala  Lys Thr Ile Lys Lys  Ala Gln Leu Leu Ile  Ile Asp Glu 
    1130                 1135                 1140             


Cys Thr  Met Ala Ser Ser His  Ala Ile Asn Ala Ile  Asp Arg Leu 
    1145                 1150                 1155             


Leu Arg  Glu Ile Met Asn Leu  Asn Val Ala Phe Gly  Gly Lys Val 
    1160                 1165                 1170             


Leu Leu  Leu Gly Gly Asp Phe  Arg Gln Cys Leu Ser  Ile Val Pro 
    1175                 1180                 1185             


His Ala  Met Arg Ser Ala Ile  Val Gln Thr Ser Leu  Lys Tyr Cys 
    1190                 1195                 1200             


Asn Val  Trp Gly Cys Phe Arg  Lys Leu Ser Leu Lys  Thr Asn Met 
    1205                 1210                 1215             


Arg Ser  Glu Asp Ser Ala Tyr  Ser Glu Trp Leu Val  Lys Leu Gly 
    1220                 1225                 1230             


Asp Gly  Lys Leu Asp Ser Ser  Phe His Leu Gly Met  Asp Ile Ile 
    1235                 1240                 1245             


Glu Ile  Pro His Glu Met Ile  Cys Asn Gly Ser Ile  Ile Glu Ala 
    1250                 1255                 1260             


Thr Phe  Gly Asn Ser Ile Ser  Ile Asp Asn Ile Lys  Asn Ile Ser 
    1265                 1270                 1275             


Lys Arg  Ala Ile Leu Cys Pro  Lys Asn Glu His Val  Gln Lys Leu 
    1280                 1285                 1290             


Asn Glu  Glu Ile Leu Asp Ile  Leu Asp Gly Asp Phe  His Thr Tyr 
    1295                 1300                 1305             


Leu Ser  Asp Asp Ser Ile Asp  Ser Thr Asp Asp Ala  Glu Lys Glu 
    1310                 1315                 1320             


Asn Phe  Pro Ile Glu Phe Leu  Asn Ser Ile Thr Pro  Ser Gly Met 
    1325                 1330                 1335             


Pro Cys  His Lys Leu Lys Leu  Lys Val Gly Ala Ile  Ile Met Leu 
    1340                 1345                 1350             


Leu Arg  Asn Leu Asn Ser Lys  Trp Gly Leu Cys Asn  Gly Thr Arg 
    1355                 1360                 1365             


Phe Ile  Ile Lys Arg Leu Arg  Pro Asn Ile Ile Glu  Ala Glu Val 
    1370                 1375                 1380             


Leu Thr  Gly Ser Ala Glu Gly  Glu Val Val Leu Ile  Pro Arg Ile 
    1385                 1390                 1395             


Asp Leu  Ser Pro Ser Asp Thr  Gly Leu Pro Phe Lys  Leu Ile Arg 
    1400                 1405                 1410             


Arg Gln  Phe Pro Val Met Pro  Ala Phe Ala Met Thr  Ile Asn Lys 
    1415                 1420                 1425             


Ser Gln  Gly Gln Thr Leu Asp  Arg Val Gly Ile Phe  Leu Pro Glu 
    1430                 1435                 1440             


Pro Val  Phe Ala His Gly Gln  Leu Tyr Val Ala Phe  Ser Arg Val 
    1445                 1450                 1455             


Arg Arg  Ala Cys Asp Val Lys  Val Lys Val Val Asn  Thr Ser Ser 
    1460                 1465                 1470             


Gln Gly  Lys Leu Val Lys His  Ser Glu Ser Val Phe  Thr Leu Asn 
    1475                 1480                 1485             


Val Val  Tyr Arg Glu Ile Leu  Glu 
    1490                 1495     


<210>  29
<211>  30
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Helraiser palindromic sequence

<400>  29
gtgcacgaat ttcgtgcacc gggccactag                                        30


<210>  30
<211>  649
<212>  PRT
<213>  Oryzias latipes

<400>  30

Met Glu Glu Val Cys Asp Ser Ser Ala Ala Ala Ser Ser Thr Val Gln 
1               5                   10                  15      


Asn Gln Pro Gln Asp Gln Glu His Pro Trp Pro Tyr Leu Arg Glu Phe 
            20                  25                  30          


Phe Ser Leu Ser Gly Val Asn Lys Asp Ser Phe Lys Met Lys Cys Val 
        35                  40                  45              


Leu Cys Leu Pro Leu Asn Lys Glu Ile Ser Ala Phe Lys Ser Ser Pro 
    50                  55                  60                  


Ser Asn Leu Arg Lys His Ile Glu Arg Met His Pro Asn Tyr Leu Lys 
65                  70                  75                  80  


Asn Tyr Ser Lys Leu Thr Ala Gln Lys Arg Lys Ile Gly Thr Ser Thr 
                85                  90                  95      


His Ala Ser Ser Ser Lys Gln Leu Lys Val Asp Ser Val Phe Pro Val 
            100                 105                 110         


Lys His Val Ser Pro Val Thr Val Asn Lys Ala Ile Leu Arg Tyr Ile 
        115                 120                 125             


Ile Gln Gly Leu His Pro Phe Ser Thr Val Asp Leu Pro Ser Phe Lys 
    130                 135                 140                 


Glu Leu Ile Ser Thr Leu Gln Pro Gly Ile Ser Val Ile Thr Arg Pro 
145                 150                 155                 160 


Thr Leu Arg Ser Lys Ile Ala Glu Ala Ala Leu Ile Met Lys Gln Lys 
                165                 170                 175     


Val Thr Ala Ala Met Ser Glu Val Glu Trp Ile Ala Thr Thr Thr Asp 
            180                 185                 190         


Cys Trp Thr Ala Arg Arg Lys Ser Phe Ile Gly Val Thr Ala His Trp 
        195                 200                 205             


Ile Asn Pro Gly Ser Leu Glu Arg His Ser Ala Ala Leu Ala Cys Lys 
    210                 215                 220                 


Arg Leu Met Gly Ser His Thr Phe Glu Val Leu Ala Ser Ala Met Asn 
225                 230                 235                 240 


Asp Ile His Ser Glu Tyr Glu Ile Arg Asp Lys Val Val Cys Thr Thr 
                245                 250                 255     


Thr Asp Ser Gly Ser Asn Phe Met Lys Ala Phe Arg Val Phe Gly Val 
            260                 265                 270         


Glu Asn Asn Asp Ile Glu Thr Glu Ala Arg Arg Cys Glu Ser Asp Asp 
        275                 280                 285             


Thr Asp Ser Glu Gly Cys Gly Glu Gly Ser Asp Gly Val Glu Phe Gln 
    290                 295                 300                 


Asp Ala Ser Arg Val Leu Asp Gln Asp Asp Gly Phe Glu Phe Gln Leu 
305                 310                 315                 320 


Pro Lys His Gln Lys Cys Ala Cys His Leu Leu Asn Leu Val Ser Ser 
                325                 330                 335     


Val Asp Ala Gln Lys Ala Leu Ser Asn Glu His Tyr Lys Lys Leu Tyr 
            340                 345                 350         


Arg Ser Val Phe Gly Lys Cys Gln Ala Leu Trp Asn Lys Ser Ser Arg 
        355                 360                 365             


Ser Ala Leu Ala Ala Glu Ala Val Glu Ser Glu Ser Arg Leu Gln Leu 
    370                 375                 380                 


Leu Arg Pro Asn Gln Thr Arg Trp Asn Ser Thr Phe Met Ala Val Asp 
385                 390                 395                 400 


Arg Ile Leu Gln Ile Cys Lys Glu Ala Gly Glu Gly Ala Leu Arg Asn 
                405                 410                 415     


Ile Cys Thr Ser Leu Glu Val Pro Met Phe Asn Pro Ala Glu Met Leu 
            420                 425                 430         


Phe Leu Thr Glu Trp Ala Asn Thr Met Arg Pro Val Ala Lys Val Leu 
        435                 440                 445             


Asp Ile Leu Gln Ala Glu Thr Asn Thr Gln Leu Gly Trp Leu Leu Pro 
    450                 455                 460                 


Ser Val His Gln Leu Ser Leu Lys Leu Gln Arg Leu His His Ser Leu 
465                 470                 475                 480 


Arg Tyr Cys Asp Pro Leu Val Asp Ala Leu Gln Gln Gly Ile Gln Thr 
                485                 490                 495     


Arg Phe Lys His Met Phe Glu Asp Pro Glu Ile Ile Ala Ala Ala Ile 
            500                 505                 510         


Leu Leu Pro Lys Phe Arg Thr Ser Trp Thr Asn Asp Glu Thr Ile Ile 
        515                 520                 525             


Lys Arg Gly Met Asp Tyr Ile Arg Val His Leu Glu Pro Leu Asp His 
    530                 535                 540                 


Lys Lys Glu Leu Ala Asn Ser Ser Ser Asp Asp Glu Asp Phe Phe Ala 
545                 550                 555                 560 


Ser Leu Lys Pro Thr Thr His Glu Ala Ser Lys Glu Leu Asp Gly Tyr 
                565                 570                 575     


Leu Ala Cys Val Ser Asp Thr Arg Glu Ser Leu Leu Thr Phe Pro Ala 
            580                 585                 590         


Ile Cys Ser Leu Ser Ile Lys Thr Asn Thr Pro Leu Pro Ala Ser Ala 
        595                 600                 605             


Ala Cys Glu Arg Leu Phe Ser Thr Ala Gly Leu Leu Phe Ser Pro Lys 
    610                 615                 620                 


Arg Ala Arg Leu Asp Thr Asn Asn Phe Glu Asn Gln Leu Leu Leu Lys 
625                 630                 635                 640 


Leu Asn Leu Arg Phe Tyr Asn Phe Glu 
                645                 


<210>  31
<211>  4682
<212>  DNA
<213>  Oryzias latipes

<400>  31
cagaggtgta aagtacttga gtaattttac ttgattactg tacttaagta ttatttttgg       60

ggatttttac tttacttgag tacaattaaa aatcaatact tttactttta cttaattaca      120

tttttttaga aaaaaaagta ctttttactc cttacaattt tatttacagt caaaaagtac      180

ttattttttg gagatcactt cattctattt tcccttgcta ttaccaaacc aattgaattg      240

cgctgatgcc cagtttaatt taaatgttat ttattctgcc tatgaaaatc gttttcacat      300

tatatgaaat tggtcagaca tgttcattgg tcctttggaa gtgacgtcat gtcacatcta      360

ttaccacaat gcacagcacc ttgacctgga aattagggaa attataacag tcaatcagtg      420

gaagaaaatg gaggaagtat gtgattcatc agcagctgcg agcagcacag tccaaaatca      480

gccacaggat caagagcacc cgtggccgta tcttcgcgaa ttcttttctt taagtggtgt      540

aaataaagat tcattcaaga tgaaatgtgt cctctgtctc ccgcttaata aagaaatatc      600

ggccttcaaa agttcgccat caaacctaag gaagcatatt gaggtaagta cattaagtat      660

tttgttttac tgatagtttt tttttttttt tttttttttt tttttgggtg tgcatgtttt      720

gacgttgatg gcgcgccttt tatatgtgta gtaggcctat tttcactaat gcatgcgatt      780

gacaatataa ggctcacgta ataaaatgct aaaatgcatt tgtaattggt aacgttaggt      840

ccacgggaaa tttggcgcct attgcagctt tgaataatca ttatcattcc gtgctctcat      900

tgtgtttgaa ttcatgcaaa acacaagaaa accaagcgag aaattttttt ccaaacatgt      960

tgtattgtca aaacggtaac actttacaat gaggttgatt agttcatgta ttaactaaca     1020

ttaaataacc atgagcaata catttgttac tgtatctgtt aatctttgtt aacgttagtt     1080

aatagaaata cagatgttca ttgtttgttc atgttagttc acagtgcatt aactaatgtt     1140

aacaagatat aaagtattag taaatgttga aattaacatg tatacgtgca gttcattatt     1200

agttcatgtt aactaatgta gttaactaac gaaccttatt gtaaaagtgt taccatcaaa     1260

actaatgtaa tgaaatcaat tcaccctgtc atgtcagcct tacagtcctg tgtttttgtc     1320

aatataatca gaaataaaat taatgtttga ttgtcactaa atgctactgt atttctaaaa     1380

tcaacaagta tttaacatta taaagtgtgc aattggctgc aaatgtcagt tttattaaag     1440

ggttagttca cccaaaaatg aaaataatgt cattaatgac tcgccctcat gtcgttccaa     1500

gcccgtaaga cctccgttca tcttcagaac acagtttaag atattttaga tttagtccga     1560

gagctttctg tgcctccatt gagaatgtat gtacggtata ctgtccatgt ccagaaaggt     1620

aataaaaaca tcaaagtagt ccatgtgaca tcagtgggtt agttagaatt ttttgaagca     1680

tcgaatacat tttggtccaa aaataacaaa acctacgact ttattcggca ttgtattctc     1740

ttccgggtct gttgtcaatc cgcgttcacg acttcgcagt gacgctacaa tgctgaataa     1800

agtcgtaggt tttgttattt ttggaccaaa atgtattttc gatgcttcaa ataattctac     1860

ctaacccact gatgtcacat ggactacttt gatgttttta ttacctttct ggacatggac     1920

agtataccgt acatacattt tcagtggagg gacagaaagc tctcggacta aatctaaaat     1980

atcttaaact gtgttccgaa gatgaacgga ggtgttacgg gcttggaacg acatgagggt     2040

gagtcattaa tgacatcttt tcatttttgg gtgaactaac cctttaatgc tgtaatcaga     2100

gagtgtatgt gtaattgtta catttattgc atacaatata aatatttatt tgttgttttt     2160

acagagaatg cacccaaatt acctcaaaaa ctactctaaa ttgacagcac agaagagaaa     2220

gatcgggacc tccacccatg cttccagcag taagcaactg aaagttgact cagttttccc     2280

agtcaaacat gtgtctccag tcactgtgaa caaagctata ttaaggtaca tcattcaagg     2340

acttcatcct ttcagcactg ttgatctgcc atcatttaaa gagctgatta gtacactgca     2400

gcctggcatt tctgtcatta caaggcctac tttacgctcc aagatagctg aagctgctct     2460

gatcatgaaa cagaaagtga ctgctgccat gagtgaagtt gaatggattg caaccacaac     2520

ggattgttgg actgcacgta gaaagtcatt cattggtgta actgctcact ggatcaaccc     2580

tggaagtctt gaaagacatt ccgctgcact tgcctgcaaa agattaatgg gctctcatac     2640

ttttgaggta ctggccagtg ccatgaatga tatccactca gagtatgaaa tacgtgacaa     2700

ggttgtttgc acaaccacag acagtggttc caactttatg aaggctttca gagtttttgg     2760

tgtggaaaac aatgatatcg agactgaggc aagaaggtgt gaaagtgatg acactgattc     2820

tgaaggctgt ggtgagggaa gtgatggtgt ggaattccaa gatgcctcac gagtcctgga     2880

ccaagacgat ggcttcgaat tccagctacc aaaacatcaa aagtgtgcct gtcacttact     2940

taacctagtc tcaagcgttg atgcccaaaa agctctctca aatgaacact acaagaaact     3000

ctacagatct gtctttggca aatgccaagc tttatggaat aaaagcagcc gatcggctct     3060

agcagctgaa gctgttgaat cagaaagccg gcttcagctt ttaaggccaa accaaacgcg     3120

gtggaattca acttttatgg ctgttgacag aattcttcaa atttgcaaag aagcaggaga     3180

aggcgcactt cggaatatat gcacctctct tgaggttcca atgtaagtgt ttttcccctc     3240

tatcgatgta aacaaatgtg ggttgttttt gtttaatact ctttgattat gctgatttct     3300

cctgtaggtt taatccagca gaaatgctgt tcttgacaga gtgggccaac acaatgcgtc     3360

cagttgcaaa agtactcgac atcttgcaag cggaaacgaa tacacagctg gggtggctgc     3420

tgcctagtgt ccatcagtta agcttgaaac ttcagcgact ccaccattct ctcaggtact     3480

gtgacccact tgtggatgcc ctacaacaag gaatccaaac acgattcaag catatgtttg     3540

aagatcctga gatcatagca gctgccatcc ttctccctaa atttcggacc tcttggacaa     3600

atgatgaaac catcataaaa cgaggtaaat gaatgcaagc aacatacact tgacgaattc     3660

taatctgggc aacctttgag ccataccaaa attattcttt tatttattta tttttgcact     3720

ttttaggaat gttatatccc atctttggct gtgatctcaa tatgaatatt gatgtaaagt     3780

attcttgcag caggttgtag ttatccctca gtgtttcttg aaaccaaact catatgtatc     3840

atatgtggtt tggaaatgca gttagatttt atgctaaaat aagggatttg catgatttta     3900

gatgtagatg actgcacgta aatgtagtta atgacaaaat ccataaaatt tgttcccagt     3960

cagaagcccc tcaaccaaac ttttctttgt gtctgctcac tgtgcttgta ggcatggact     4020

acatcagagt gcatctggag cctttggacc acaagaagga attggccaac agttcatctg     4080

atgatgaaga ttttttcgct tctttgaaac cgacaacaca tgaagccagc aaagagttgg     4140

atggatatct ggcctgtgtt tcagacacca gggagtctct gctcacgttt cctgctattt     4200

gcagcctctc tatcaagact aatacacctc ttcccgcatc ggctgcctgt gagaggcttt     4260

tcagcactgc aggattgctt ttcagcccca aaagagctag gcttgacact aacaattttg     4320

agaatcagct tctactgaag ttaaatctga ggttttacaa ctttgagtag cgtgtactgg     4380

cattagattg tctgtcttat agtttgataa ttaaatacaa acagttctaa agcaggataa     4440

aaccttgtat gcatttcatt taatgttttt tgagattaaa agcttaaaca agaatctcta     4500

gttttctttc ttgcttttac ttttacttcc ttaatactca agtacaattt taatggagta     4560

cttttttact tttactcaag taagattcta gccagatact tttactttta attgagtaaa     4620

attttcccta agtacttgta ctttcacttg agtaaaattt ttgagtactt tttacacctc     4680

tg                                                                    4682


<210>  32
<211>  1053
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  dSaCas9

<400>  32

Met Lys Arg Asn Tyr Ile Leu Gly Leu Ala Ile Gly Ile Thr Ser Val 
1               5                   10                  15      


Gly Tyr Gly Ile Ile Asp Tyr Glu Thr Arg Asp Val Ile Asp Ala Gly 
            20                  25                  30          


Val Arg Leu Phe Lys Glu Ala Asn Val Glu Asn Asn Glu Gly Arg Arg 
        35                  40                  45              


Ser Lys Arg Gly Ala Arg Arg Leu Lys Arg Arg Arg Arg His Arg Ile 
    50                  55                  60                  


Gln Arg Val Lys Lys Leu Leu Phe Asp Tyr Asn Leu Leu Thr Asp His 
65                  70                  75                  80  


Ser Glu Leu Ser Gly Ile Asn Pro Tyr Glu Ala Arg Val Lys Gly Leu 
                85                  90                  95      


Ser Gln Lys Leu Ser Glu Glu Glu Phe Ser Ala Ala Leu Leu His Leu 
            100                 105                 110         


Ala Lys Arg Arg Gly Val His Asn Val Asn Glu Val Glu Glu Asp Thr 
        115                 120                 125             


Gly Asn Glu Leu Ser Thr Lys Glu Gln Ile Ser Arg Asn Ser Lys Ala 
    130                 135                 140                 


Leu Glu Glu Lys Tyr Val Ala Glu Leu Gln Leu Glu Arg Leu Lys Lys 
145                 150                 155                 160 


Asp Gly Glu Val Arg Gly Ser Ile Asn Arg Phe Lys Thr Ser Asp Tyr 
                165                 170                 175     


Val Lys Glu Ala Lys Gln Leu Leu Lys Val Gln Lys Ala Tyr His Gln 
            180                 185                 190         


Leu Asp Gln Ser Phe Ile Asp Thr Tyr Ile Asp Leu Leu Glu Thr Arg 
        195                 200                 205             


Arg Thr Tyr Tyr Glu Gly Pro Gly Glu Gly Ser Pro Phe Gly Trp Lys 
    210                 215                 220                 


Asp Ile Lys Glu Trp Tyr Glu Met Leu Met Gly His Cys Thr Tyr Phe 
225                 230                 235                 240 


Pro Glu Glu Leu Arg Ser Val Lys Tyr Ala Tyr Asn Ala Asp Leu Tyr 
                245                 250                 255     


Asn Ala Leu Asn Asp Leu Asn Asn Leu Val Ile Thr Arg Asp Glu Asn 
            260                 265                 270         


Glu Lys Leu Glu Tyr Tyr Glu Lys Phe Gln Ile Ile Glu Asn Val Phe 
        275                 280                 285             


Lys Gln Lys Lys Lys Pro Thr Leu Lys Gln Ile Ala Lys Glu Ile Leu 
    290                 295                 300                 


Val Asn Glu Glu Asp Ile Lys Gly Tyr Arg Val Thr Ser Thr Gly Lys 
305                 310                 315                 320 


Pro Glu Phe Thr Asn Leu Lys Val Tyr His Asp Ile Lys Asp Ile Thr 
                325                 330                 335     


Ala Arg Lys Glu Ile Ile Glu Asn Ala Glu Leu Leu Asp Gln Ile Ala 
            340                 345                 350         


Lys Ile Leu Thr Ile Tyr Gln Ser Ser Glu Asp Ile Gln Glu Glu Leu 
        355                 360                 365             


Thr Asn Leu Asn Ser Glu Leu Thr Gln Glu Glu Ile Glu Gln Ile Ser 
    370                 375                 380                 


Asn Leu Lys Gly Tyr Thr Gly Thr His Asn Leu Ser Leu Lys Ala Ile 
385                 390                 395                 400 


Asn Leu Ile Leu Asp Glu Leu Trp His Thr Asn Asp Asn Gln Ile Ala 
                405                 410                 415     


Ile Phe Asn Arg Leu Lys Leu Val Pro Lys Lys Val Asp Leu Ser Gln 
            420                 425                 430         


Gln Lys Glu Ile Pro Thr Thr Leu Val Asp Asp Phe Ile Leu Ser Pro 
        435                 440                 445             


Val Val Lys Arg Ser Phe Ile Gln Ser Ile Lys Val Ile Asn Ala Ile 
    450                 455                 460                 


Ile Lys Lys Tyr Gly Leu Pro Asn Asp Ile Ile Ile Glu Leu Ala Arg 
465                 470                 475                 480 


Glu Lys Asn Ser Lys Asp Ala Gln Lys Met Ile Asn Glu Met Gln Lys 
                485                 490                 495     


Arg Asn Arg Gln Thr Asn Glu Arg Ile Glu Glu Ile Ile Arg Thr Thr 
            500                 505                 510         


Gly Lys Glu Asn Ala Lys Tyr Leu Ile Glu Lys Ile Lys Leu His Asp 
        515                 520                 525             


Met Gln Glu Gly Lys Cys Leu Tyr Ser Leu Glu Ala Ile Pro Leu Glu 
    530                 535                 540                 


Asp Leu Leu Asn Asn Pro Phe Asn Tyr Glu Val Asp His Ile Ile Pro 
545                 550                 555                 560 


Arg Ser Val Ser Phe Asp Asn Ser Phe Asn Asn Lys Val Leu Val Lys 
                565                 570                 575     


Gln Glu Glu Ala Ser Lys Lys Gly Asn Arg Thr Pro Phe Gln Tyr Leu 
            580                 585                 590         


Ser Ser Ser Asp Ser Lys Ile Ser Tyr Glu Thr Phe Lys Lys His Ile 
        595                 600                 605             


Leu Asn Leu Ala Lys Gly Lys Gly Arg Ile Ser Lys Thr Lys Lys Glu 
    610                 615                 620                 


Tyr Leu Leu Glu Glu Arg Asp Ile Asn Arg Phe Ser Val Gln Lys Asp 
625                 630                 635                 640 


Phe Ile Asn Arg Asn Leu Val Asp Thr Arg Tyr Ala Thr Arg Gly Leu 
                645                 650                 655     


Met Asn Leu Leu Arg Ser Tyr Phe Arg Val Asn Asn Leu Asp Val Lys 
            660                 665                 670         


Val Lys Ser Ile Asn Gly Gly Phe Thr Ser Phe Leu Arg Arg Lys Trp 
        675                 680                 685             


Lys Phe Lys Lys Glu Arg Asn Lys Gly Tyr Lys His His Ala Glu Asp 
    690                 695                 700                 


Ala Leu Ile Ile Ala Asn Ala Asp Phe Ile Phe Lys Glu Trp Lys Lys 
705                 710                 715                 720 


Leu Asp Lys Ala Lys Lys Val Met Glu Asn Gln Met Phe Glu Glu Lys 
                725                 730                 735     


Gln Ala Glu Ser Met Pro Glu Ile Glu Thr Glu Gln Glu Tyr Lys Glu 
            740                 745                 750         


Ile Phe Ile Thr Pro His Gln Ile Lys His Ile Lys Asp Phe Lys Asp 
        755                 760                 765             


Tyr Lys Tyr Ser His Arg Val Asp Lys Lys Pro Asn Arg Glu Leu Ile 
    770                 775                 780                 


Asn Asp Thr Leu Tyr Ser Thr Arg Lys Asp Asp Lys Gly Asn Thr Leu 
785                 790                 795                 800 


Ile Val Asn Asn Leu Asn Gly Leu Tyr Asp Lys Asp Asn Asp Lys Leu 
                805                 810                 815     


Lys Lys Leu Ile Asn Lys Ser Pro Glu Lys Leu Leu Met Tyr His His 
            820                 825                 830         


Asp Pro Gln Thr Tyr Gln Lys Leu Lys Leu Ile Met Glu Gln Tyr Gly 
        835                 840                 845             


Asp Glu Lys Asn Pro Leu Tyr Lys Tyr Tyr Glu Glu Thr Gly Asn Tyr 
    850                 855                 860                 


Leu Thr Lys Tyr Ser Lys Lys Asp Asn Gly Pro Val Ile Lys Lys Ile 
865                 870                 875                 880 


Lys Tyr Tyr Gly Asn Lys Leu Asn Ala His Leu Asp Ile Thr Asp Asp 
                885                 890                 895     


Tyr Pro Asn Ser Arg Asn Lys Val Val Lys Leu Ser Leu Lys Pro Tyr 
            900                 905                 910         


Arg Phe Asp Val Tyr Leu Asp Asn Gly Val Tyr Lys Phe Val Thr Val 
        915                 920                 925             


Lys Asn Leu Asp Val Ile Lys Lys Glu Asn Tyr Tyr Glu Val Asn Ser 
    930                 935                 940                 


Lys Cys Tyr Glu Glu Ala Lys Lys Leu Lys Lys Ile Ser Asn Gln Ala 
945                 950                 955                 960 


Glu Phe Ile Ala Ser Phe Tyr Asn Asn Asp Leu Ile Lys Ile Asn Gly 
                965                 970                 975     


Glu Leu Tyr Arg Val Ile Gly Val Asn Asn Asp Leu Leu Asn Arg Ile 
            980                 985                 990         


Glu Val Asn Met Ile Asp Ile Thr  Tyr Arg Glu Tyr Leu  Glu Asn Met 
        995                 1000                 1005             


Asn Asp  Lys Arg Pro Pro Arg  Ile Ile Lys Thr Ile  Ala Ser Lys 
    1010                 1015                 1020             


Thr Gln  Ser Ile Lys Lys Tyr  Ser Thr Asp Ile Leu  Gly Asn Leu 
    1025                 1030                 1035             


Tyr Glu  Val Lys Ser Lys Lys  His Pro Gln Ile Ile  Lys Lys Gly 
    1040                 1045                 1050             


<210>  33
<211>  1368
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  dCas9 with X at position 1, X can be any amino acid


<220>
<221>  misc_feature
<222>  (1)..(1)
<223>  Xaa can be any naturally occurring amino acid

<400>  33

Xaa Asp Lys Lys Tyr Ser Ile Gly Leu Ala Ile Gly Thr Asn Ser Val 
1               5                   10                  15      


Gly Trp Ala Val Ile Thr Asp Glu Tyr Lys Val Pro Ser Lys Lys Phe 
            20                  25                  30          


Lys Val Leu Gly Asn Thr Asp Arg His Ser Ile Lys Lys Asn Leu Ile 
        35                  40                  45              


Gly Ala Leu Leu Phe Asp Ser Gly Glu Thr Ala Glu Ala Thr Arg Leu 
    50                  55                  60                  


Lys Arg Thr Ala Arg Arg Arg Tyr Thr Arg Arg Lys Asn Arg Ile Cys 
65                  70                  75                  80  


Tyr Leu Gln Glu Ile Phe Ser Asn Glu Met Ala Lys Val Asp Asp Ser 
                85                  90                  95      


Phe Phe His Arg Leu Glu Glu Ser Phe Leu Val Glu Glu Asp Lys Lys 
            100                 105                 110         


His Glu Arg His Pro Ile Phe Gly Asn Ile Val Asp Glu Val Ala Tyr 
        115                 120                 125             


His Glu Lys Tyr Pro Thr Ile Tyr His Leu Arg Lys Lys Leu Val Asp 
    130                 135                 140                 


Ser Thr Asp Lys Ala Asp Leu Arg Leu Ile Tyr Leu Ala Leu Ala His 
145                 150                 155                 160 


Met Ile Lys Phe Arg Gly His Phe Leu Ile Glu Gly Asp Leu Asn Pro 
                165                 170                 175     


Asp Asn Ser Asp Val Asp Lys Leu Phe Ile Gln Leu Val Gln Thr Tyr 
            180                 185                 190         


Asn Gln Leu Phe Glu Glu Asn Pro Ile Asn Ala Ser Gly Val Asp Ala 
        195                 200                 205             


Lys Ala Ile Leu Ser Ala Arg Leu Ser Lys Ser Arg Arg Leu Glu Asn 
    210                 215                 220                 


Leu Ile Ala Gln Leu Pro Gly Glu Lys Lys Asn Gly Leu Phe Gly Asn 
225                 230                 235                 240 


Leu Ile Ala Leu Ser Leu Gly Leu Thr Pro Asn Phe Lys Ser Asn Phe 
                245                 250                 255     


Asp Leu Ala Glu Asp Ala Lys Leu Gln Leu Ser Lys Asp Thr Tyr Asp 
            260                 265                 270         


Asp Asp Leu Asp Asn Leu Leu Ala Gln Ile Gly Asp Gln Tyr Ala Asp 
        275                 280                 285             


Leu Phe Leu Ala Ala Lys Asn Leu Ser Asp Ala Ile Leu Leu Ser Asp 
    290                 295                 300                 


Ile Leu Arg Val Asn Thr Glu Ile Thr Lys Ala Pro Leu Ser Ala Ser 
305                 310                 315                 320 


Met Ile Lys Arg Tyr Asp Glu His His Gln Asp Leu Thr Leu Leu Lys 
                325                 330                 335     


Ala Leu Val Arg Gln Gln Leu Pro Glu Lys Tyr Lys Glu Ile Phe Phe 
            340                 345                 350         


Asp Gln Ser Lys Asn Gly Tyr Ala Gly Tyr Ile Asp Gly Gly Ala Ser 
        355                 360                 365             


Gln Glu Glu Phe Tyr Lys Phe Ile Lys Pro Ile Leu Glu Lys Met Asp 
    370                 375                 380                 


Gly Thr Glu Glu Leu Leu Val Lys Leu Asn Arg Glu Asp Leu Leu Arg 
385                 390                 395                 400 


Lys Gln Arg Thr Phe Asp Asn Gly Ser Ile Pro His Gln Ile His Leu 
                405                 410                 415     


Gly Glu Leu His Ala Ile Leu Arg Arg Gln Glu Asp Phe Tyr Pro Phe 
            420                 425                 430         


Leu Lys Asp Asn Arg Glu Lys Ile Glu Lys Ile Leu Thr Phe Arg Ile 
        435                 440                 445             


Pro Tyr Tyr Val Gly Pro Leu Ala Arg Gly Asn Ser Arg Phe Ala Trp 
    450                 455                 460                 


Met Thr Arg Lys Ser Glu Glu Thr Ile Thr Pro Trp Asn Phe Glu Glu 
465                 470                 475                 480 


Val Val Asp Lys Gly Ala Ser Ala Gln Ser Phe Ile Glu Arg Met Thr 
                485                 490                 495     


Asn Phe Asp Lys Asn Leu Pro Asn Glu Lys Val Leu Pro Lys His Ser 
            500                 505                 510         


Leu Leu Tyr Glu Tyr Phe Thr Val Tyr Asn Glu Leu Thr Lys Val Lys 
        515                 520                 525             


Tyr Val Thr Glu Gly Met Arg Lys Pro Ala Phe Leu Ser Gly Glu Gln 
    530                 535                 540                 


Lys Lys Ala Ile Val Asp Leu Leu Phe Lys Thr Asn Arg Lys Val Thr 
545                 550                 555                 560 


Val Lys Gln Leu Lys Glu Asp Tyr Phe Lys Lys Ile Glu Cys Phe Asp 
                565                 570                 575     


Ser Val Glu Ile Ser Gly Val Glu Asp Arg Phe Asn Ala Ser Leu Gly 
            580                 585                 590         


Thr Tyr His Asp Leu Leu Lys Ile Ile Lys Asp Lys Asp Phe Leu Asp 
        595                 600                 605             


Asn Glu Glu Asn Glu Asp Ile Leu Glu Asp Ile Val Leu Thr Leu Thr 
    610                 615                 620                 


Leu Phe Glu Asp Arg Glu Met Ile Glu Glu Arg Leu Lys Thr Tyr Ala 
625                 630                 635                 640 


His Leu Phe Asp Asp Lys Val Met Lys Gln Leu Lys Arg Arg Arg Tyr 
                645                 650                 655     


Thr Gly Trp Gly Arg Leu Ser Arg Lys Leu Ile Asn Gly Ile Arg Asp 
            660                 665                 670         


Lys Gln Ser Gly Lys Thr Ile Leu Asp Phe Leu Lys Ser Asp Gly Phe 
        675                 680                 685             


Ala Asn Arg Asn Phe Met Gln Leu Ile His Asp Asp Ser Leu Thr Phe 
    690                 695                 700                 


Lys Glu Asp Ile Gln Lys Ala Gln Val Ser Gly Gln Gly Asp Ser Leu 
705                 710                 715                 720 


His Glu His Ile Ala Asn Leu Ala Gly Ser Pro Ala Ile Lys Lys Gly 
                725                 730                 735     


Ile Leu Gln Thr Val Lys Val Val Asp Glu Leu Val Lys Val Met Gly 
            740                 745                 750         


Arg His Lys Pro Glu Asn Ile Val Ile Glu Met Ala Arg Glu Asn Gln 
        755                 760                 765             


Thr Thr Gln Lys Gly Gln Lys Asn Ser Arg Glu Arg Met Lys Arg Ile 
    770                 775                 780                 


Glu Glu Gly Ile Lys Glu Leu Gly Ser Gln Ile Leu Lys Glu His Pro 
785                 790                 795                 800 


Val Glu Asn Thr Gln Leu Gln Asn Glu Lys Leu Tyr Leu Tyr Tyr Leu 
                805                 810                 815     


Gln Asn Gly Arg Asp Met Tyr Val Asp Gln Glu Leu Asp Ile Asn Arg 
            820                 825                 830         


Leu Ser Asp Tyr Asp Val Asp Ala Ile Val Pro Gln Ser Phe Leu Lys 
        835                 840                 845             


Asp Asp Ser Ile Asp Asn Lys Val Leu Thr Arg Ser Asp Lys Asn Arg 
    850                 855                 860                 


Gly Lys Ser Asp Asn Val Pro Ser Glu Glu Val Val Lys Lys Met Lys 
865                 870                 875                 880 


Asn Tyr Trp Arg Gln Leu Leu Asn Ala Lys Leu Ile Thr Gln Arg Lys 
                885                 890                 895     


Phe Asp Asn Leu Thr Lys Ala Glu Arg Gly Gly Leu Ser Glu Leu Asp 
            900                 905                 910         


Lys Ala Gly Phe Ile Lys Arg Gln Leu Val Glu Thr Arg Gln Ile Thr 
        915                 920                 925             


Lys His Val Ala Gln Ile Leu Asp Ser Arg Met Asn Thr Lys Tyr Asp 
    930                 935                 940                 


Glu Asn Asp Lys Leu Ile Arg Glu Val Lys Val Ile Thr Leu Lys Ser 
945                 950                 955                 960 


Lys Leu Val Ser Asp Phe Arg Lys Asp Phe Gln Phe Tyr Lys Val Arg 
                965                 970                 975     


Glu Ile Asn Asn Tyr His His Ala His Asp Ala Tyr Leu Asn Ala Val 
            980                 985                 990         


Val Gly Thr Ala Leu Ile Lys Lys  Tyr Pro Lys Leu Glu  Ser Glu Phe 
        995                 1000                 1005             


Val Tyr  Gly Asp Tyr Lys Val  Tyr Asp Val Arg Lys  Met Ile Ala 
    1010                 1015                 1020             


Lys Ser  Glu Gln Glu Ile Gly  Lys Ala Thr Ala Lys  Tyr Phe Phe 
    1025                 1030                 1035             


Tyr Ser  Asn Ile Met Asn Phe  Phe Lys Thr Glu Ile  Thr Leu Ala 
    1040                 1045                 1050             


Asn Gly  Glu Ile Arg Lys Arg  Pro Leu Ile Glu Thr  Asn Gly Glu 
    1055                 1060                 1065             


Thr Gly  Glu Ile Val Trp Asp  Lys Gly Arg Asp Phe  Ala Thr Val 
    1070                 1075                 1080             


Arg Lys  Val Leu Ser Met Pro  Gln Val Asn Ile Val  Lys Lys Thr 
    1085                 1090                 1095             


Glu Val  Gln Thr Gly Gly Phe  Ser Lys Glu Ser Ile  Leu Pro Lys 
    1100                 1105                 1110             


Arg Asn  Ser Asp Lys Leu Ile  Ala Arg Lys Lys Asp  Trp Asp Pro 
    1115                 1120                 1125             


Lys Lys  Tyr Gly Gly Phe Asp  Ser Pro Thr Val Ala  Tyr Ser Val 
    1130                 1135                 1140             


Leu Val  Val Ala Lys Val Glu  Lys Gly Lys Ser Lys  Lys Leu Lys 
    1145                 1150                 1155             


Ser Val  Lys Glu Leu Leu Gly  Ile Thr Ile Met Glu  Arg Ser Ser 
    1160                 1165                 1170             


Phe Glu  Lys Asn Pro Ile Asp  Phe Leu Glu Ala Lys  Gly Tyr Lys 
    1175                 1180                 1185             


Glu Val  Lys Lys Asp Leu Ile  Ile Lys Leu Pro Lys  Tyr Ser Leu 
    1190                 1195                 1200             


Phe Glu  Leu Glu Asn Gly Arg  Lys Arg Met Leu Ala  Ser Ala Gly 
    1205                 1210                 1215             


Glu Leu  Gln Lys Gly Asn Glu  Leu Ala Leu Pro Ser  Lys Tyr Val 
    1220                 1225                 1230             


Asn Phe  Leu Tyr Leu Ala Ser  His Tyr Glu Lys Leu  Lys Gly Ser 
    1235                 1240                 1245             


Pro Glu  Asp Asn Glu Gln Lys  Gln Leu Phe Val Glu  Gln His Lys 
    1250                 1255                 1260             


His Tyr  Leu Asp Glu Ile Ile  Glu Gln Ile Ser Glu  Phe Ser Lys 
    1265                 1270                 1275             


Arg Val  Ile Leu Ala Asp Ala  Asn Leu Asp Lys Val  Leu Ser Ala 
    1280                 1285                 1290             


Tyr Asn  Lys His Arg Asp Lys  Pro Ile Arg Glu Gln  Ala Glu Asn 
    1295                 1300                 1305             


Ile Ile  His Leu Phe Thr Leu  Thr Asn Leu Gly Ala  Pro Ala Ala 
    1310                 1315                 1320             


Phe Lys  Tyr Phe Asp Thr Thr  Ile Asp Arg Lys Arg  Tyr Thr Ser 
    1325                 1330                 1335             


Thr Lys  Glu Val Leu Asp Ala  Thr Leu Ile His Gln  Ser Ile Thr 
    1340                 1345                 1350             


Gly Leu  Tyr Glu Thr Arg Ile  Asp Leu Ser Gln Leu  Gly Gly Asp 
    1355                 1360                 1365             


<210>  34
<211>  199
<212>  PRT
<213>  Clostridium sp. 7_2_43FAA

<400>  34

Glu Gly Ile Lys Ser Asn Ile Ser Leu Leu Lys Asp Glu Leu Arg Gly 
1               5                   10                  15      


Gln Ile Ser His Ile Ser His Glu Tyr Leu Ser Leu Ile Asp Leu Ala 
            20                  25                  30          


Phe Asp Ser Lys Gln Asn Arg Leu Phe Glu Met Lys Val Leu Glu Leu 
        35                  40                  45              


Leu Val Asn Glu Tyr Gly Phe Lys Gly Arg His Leu Gly Gly Ser Arg 
    50                  55                  60                  


Lys Pro Asp Gly Ile Val Tyr Ser Thr Thr Leu Glu Asp Asn Phe Gly 
65                  70                  75                  80  


Ile Ile Val Asp Thr Lys Ala Tyr Ser Glu Gly Tyr Ser Leu Pro Ile 
                85                  90                  95      


Ser Gln Ala Asp Glu Met Glu Arg Tyr Val Arg Glu Asn Ser Asn Arg 
            100                 105                 110         


Asp Glu Glu Val Asn Pro Asn Lys Trp Trp Glu Asn Phe Ser Glu Glu 
        115                 120                 125             


Val Lys Lys Tyr Tyr Phe Val Phe Ile Ser Gly Ser Phe Lys Gly Lys 
    130                 135                 140                 


Phe Glu Glu Gln Leu Arg Arg Leu Ser Met Thr Thr Gly Val Asn Gly 
145                 150                 155                 160 


Ser Ala Val Asn Val Val Asn Leu Leu Leu Gly Ala Glu Lys Ile Arg 
                165                 170                 175     


Ser Gly Glu Met Thr Ile Glu Glu Leu Glu Arg Ala Met Phe Asn Asn 
            180                 185                 190         


Ser Glu Phe Ile Leu Lys Tyr 
        195                 


<210>  35
<211>  4897
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PB-EF1a vector

<400>  35
tgtacataga ttaaccctag aaagataatc atattgtgac gtacgttaaa gataatcatg       60

cgtaaaattg acgcatgtgt tttatcggtc tgtatatcga ggtttattta ttaatttgaa      120

tagatattaa gttttattat atttacactt acatactaat aataaattca acaaacaatt      180

tatttatgtt tatttattta ttaaaaaaaa acaaaaactc aaaatttctt ctataaagta      240

acaaaacttt tatcgaatac ctgcagcccg ggggatgcag agggacagcc cccccccaaa      300

gcccccaggg atgtaattac gtccctcccc cgctaggggg cagcagcgag ccgcccgggg      360

ctccgctccg gtccggcgct ccccccgcat ccccgagccg gcagcgtgcg gggacagccc      420

gggcacgggg aaggtggcac gggatcgctt tcctctgaac gcttctcgct gctctttgag      480

cctgcagaca cctgggggga tacggggaaa agttgactgt gcctttcgat cgaaccatgg      540

acagttagct ttgcaaagat ggataaagtt ttaaacagag aggaatcttt gcagctaatg      600

gaccttctag gtcttgaaag gagtgggaat tggctccggt gcccgtcagt gggcagagcg      660

cacatcgccc acagtccccg agaagttggg gggaggggtc ggcaattgaa ccggtgccta      720

gagaaggtgg cgcggggtaa actgggaaag tgatgtcgtg tactggctcc gcctttttcc      780

cgagggtggg ggagaaccgt atataagtgc agtagtcgcc gtgaacgttc tttttcgcaa      840

cgggtttgcc gccagaacac aggtaagtgc cgtgtgtggt tcccgcgggc ctggcctctt      900

tacgggttat ggcccttgcg tgccttgaat tacttccacc tggctgcagt acgtgattct      960

tgatcccgag cttcgggttg gaagtgggtg ggagagttcg aggccttgcg cttaaggagc     1020

cccttcgcct cgtgcttgag ttgaggcctg gcctgggcgc tggggccgcc gcgtgcgaat     1080

ctggtggcac cttcgcgcct gtctcgctgc tttcgataag tctctagcca tttaaaattt     1140

ttgatgacct gctgcgacgc tttttttctg gcaagatagt cttgtaaatg cgggccaaga     1200

tctgcacact ggtatttcgg tttttggggc cgcgggcggc gacggggccc gtgcgtccca     1260

gcgcacatgt tcggcgaggc ggggcctgcg agcgcggcca ccgagaatcg gacgggggta     1320

gtctcaagct ggccggcctg ctctggtgcc tggcctcgcg ccgccgtgta tcgccccgcc     1380

ctgggcggca aggctggccc ggtcggcacc agttgcgtga gcggaaagat ggccgcttcc     1440

cggccctgct gcagggagct caaaatggag gacgcggcgc tcgggagagc gggcgggtga     1500

gtcacccaca caaaggaaaa gggcctttcc gtcctcagcc gtcgcttcat gtgactccac     1560

ggagtaccgg gcgccgtcca ggcacctcga ttagttctcg agcttttgga gtacgtcgtc     1620

tttaggttgg ggggaggggt tttatgcgat ggagtttccc cacactgagt gggtggagac     1680

tgaagttagg ccagcttggc acttgatgta attctccttg gaatttgccc tttttgagtt     1740

tggatcttgg ttcattctca agcctcagac agtggttcaa agtttttttc ttccatttca     1800

ggtgtcgtga gaattctaat acgactcact atagggtgtg ctgtctcatc attttggcaa     1860

agattggcca ccaagcttgt cctgcaggag ggtcgacgcc tctagacggg cggccgctcc     1920

ggatccacgg gtaccgatca catatgcctt taattaaaca ctagttctat agtgtcacct     1980

aaattccctt tagtgagggt taatggccgt aggccgccag aattgggtcc agacatgata     2040

agatacattg atgagtttgg acaaaccaca actagaatgc agtgaaaaaa atgctttatt     2100

tgtgaaattt gtgatgctat tgctttattt gtaaccatta taagctgcaa taaacaagtt     2160

aacaacaaca attgcattca ttttatgttt caggttcagg gggaggtgtg ggaggttttt     2220

tcggactcta ggacctgcgc atgcgcttgg cgtaatcatg gtcatagctg tttcctgttt     2280

tccccgtatc cccccaggtg tctgcaggct caaagagcag cgagaagcgt tcagaggaaa     2340

gcgatcccgt gccaccttcc ccgtgcccgg gctgtccccg cacgctgccg gctcggggat     2400

gcggggggag cgccggaccg gagcggagcc ccgggcggct cgctgctgcc ccctagcggg     2460

ggagggacgt aattacatcc ctgggggctt tggggggggg ctgtccctct caccgcggtg     2520

gagctccagc ttttgttcga attggggccc cccctcgagg gtatcgatga tatctataac     2580

aagaaaatat atatataata agttatcacg taagtagaac atgaaataac aatataatta     2640

tcgtatgagt taaatcttaa aagtcacgta aaagataatc atgcgtcatt ttgactcacg     2700

cggtcgttat agttcaaaat cagtgacact taccgcattg acaagcacgc ctcacgggag     2760

ctccaagcgg cgactgagat gtcctaaatg cacagcgacg gattcgcgct atttagaaag     2820

agagagcaat atttcaagaa tgcatgcgtc aattttacgc agactatctt tctagggtta     2880

atctagctag ccttaagggc gcctattgcg ttgcgctcac tgcccgcttt ccagtcggga     2940

aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt     3000

attgggcgct cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg     3060

cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac     3120

gcaggaaaga acatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac     3180

cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc     3240

ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca     3300

actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgttcttcta     3360

gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct     3420

ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg     3480

gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc     3540

acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta     3600

tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg     3660

gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt     3720

cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg     3780

cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg     3840

ccttttgctc acatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa     3900

tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag tcagaagaac     3960

tcgtcaagaa ggcgatagaa ggcgatgcgc tgcgaatcgg gagcggcgat accgtaaagc     4020

acgaggaagc ggtcagccca ttcgccgcca agctcttcag caatatcacg ggtagccaac     4080

gctatgtcct gatagcggtc cgccacaccc agccggccac agtcgatgaa tccagaaaag     4140

cggccatttt ccaccatgat attcggcaag caggcatcgc catgggtcac gacgagatcc     4200

tcgccgtcgg gcatgctcgc cttgagcctg gcgaacagtt cggctggcgc gagcccctga     4260

tgctcttcgt ccagatcatc ctgatcgaca agaccggctt ccatccgagt acgtgctcgc     4320

tcgatgcgat gtttcgcttg gtggtcgaat gggcaggtag ccggatcaag cgtatgcagc     4380

cgccgcattg catcagccat gatggatact ttctcggcag gagcaaggtg agatgacagg     4440

agatcctgcc ccggcacttc gcccaatagc agccagtccc ttcccgcttc agtgacaacg     4500

tcgagcacag ctgcgcaagg aacgcccgtc gtggccagcc acgatagccg cgctgcctcg     4560

tcttgcagtt cattcagggc accggacagg tcggtcttga caaaaagaac cgggcgcccc     4620

tgcgctgaca gccggaacac ggcggcatca gagcagccga ttgtctgttg tgcccagtca     4680

tagccgaata gcctctccac ccaagcggcc ggagaacctg cgtgcaatcc atcttgttca     4740

atcataatat tattgaagca tttatcaggg ttcgtctcgt cccggtctcc tcccaatgca     4800

tgtcaatatt ggccattagc catattattc attggttata tagcataaat caatattggc     4860

tattggccat tgcatacgtt gtatctatat cataata                              4897


<210>  36
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  EF1a-2r primer

<400>  36
caccggagcc aattcccact                                                   20


<210>  37
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  AAVS-3r primer

<400>  37
ctgcaccacg tgatgtcctc                                                   20


<210>  38
<211>  28
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  SV40pA-1r primer

<400>  38
gtaaccatta taagctgcaa taaacaag                                          28


<210>  39
<211>  24
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  AAVS-2f primer

<400>  39
ctggggactc tttaaggaaa gaag                                              24


<210>  40
<211>  1591
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  dCas9-Clo051

<400>  40

Met Ala Pro Lys Lys Lys Arg Lys Val Glu Gly Ile Lys Ser Asn Ile 
1               5                   10                  15      


Ser Leu Leu Lys Asp Glu Leu Arg Gly Gln Ile Ser His Ile Ser His 
            20                  25                  30          


Glu Tyr Leu Ser Leu Ile Asp Leu Ala Phe Asp Ser Lys Gln Asn Arg 
        35                  40                  45              


Leu Phe Glu Met Lys Val Leu Glu Leu Leu Val Asn Glu Tyr Gly Phe 
    50                  55                  60                  


Lys Gly Arg His Leu Gly Gly Ser Arg Lys Pro Asp Gly Ile Val Tyr 
65                  70                  75                  80  


Ser Thr Thr Leu Glu Asp Asn Phe Gly Ile Ile Val Asp Thr Lys Ala 
                85                  90                  95      


Tyr Ser Glu Gly Tyr Ser Leu Pro Ile Ser Gln Ala Asp Glu Met Glu 
            100                 105                 110         


Arg Tyr Val Arg Glu Asn Ser Asn Arg Asp Glu Glu Val Asn Pro Asn 
        115                 120                 125             


Lys Trp Trp Glu Asn Phe Ser Glu Glu Val Lys Lys Tyr Tyr Phe Val 
    130                 135                 140                 


Phe Ile Ser Gly Ser Phe Lys Gly Lys Phe Glu Glu Gln Leu Arg Arg 
145                 150                 155                 160 


Leu Ser Met Thr Thr Gly Val Asn Gly Ser Ala Val Asn Val Val Asn 
                165                 170                 175     


Leu Leu Leu Gly Ala Glu Lys Ile Arg Ser Gly Glu Met Thr Ile Glu 
            180                 185                 190         


Glu Leu Glu Arg Ala Met Phe Asn Asn Ser Glu Phe Ile Leu Lys Tyr 
        195                 200                 205             


Gly Gly Gly Gly Ser Asp Lys Lys Tyr Ser Ile Gly Leu Ala Ile Gly 
    210                 215                 220                 


Thr Asn Ser Val Gly Trp Ala Val Ile Thr Asp Glu Tyr Lys Val Pro 
225                 230                 235                 240 


Ser Lys Lys Phe Lys Val Leu Gly Asn Thr Asp Arg His Ser Ile Lys 
                245                 250                 255     


Lys Asn Leu Ile Gly Ala Leu Leu Phe Asp Ser Gly Glu Thr Ala Glu 
            260                 265                 270         


Ala Thr Arg Leu Lys Arg Thr Ala Arg Arg Arg Tyr Thr Arg Arg Lys 
        275                 280                 285             


Asn Arg Ile Cys Tyr Leu Gln Glu Ile Phe Ser Asn Glu Met Ala Lys 
    290                 295                 300                 


Val Asp Asp Ser Phe Phe His Arg Leu Glu Glu Ser Phe Leu Val Glu 
305                 310                 315                 320 


Glu Asp Lys Lys His Glu Arg His Pro Ile Phe Gly Asn Ile Val Asp 
                325                 330                 335     


Glu Val Ala Tyr His Glu Lys Tyr Pro Thr Ile Tyr His Leu Arg Lys 
            340                 345                 350         


Lys Leu Val Asp Ser Thr Asp Lys Ala Asp Leu Arg Leu Ile Tyr Leu 
        355                 360                 365             


Ala Leu Ala His Met Ile Lys Phe Arg Gly His Phe Leu Ile Glu Gly 
    370                 375                 380                 


Asp Leu Asn Pro Asp Asn Ser Asp Val Asp Lys Leu Phe Ile Gln Leu 
385                 390                 395                 400 


Val Gln Thr Tyr Asn Gln Leu Phe Glu Glu Asn Pro Ile Asn Ala Ser 
                405                 410                 415     


Gly Val Asp Ala Lys Ala Ile Leu Ser Ala Arg Leu Ser Lys Ser Arg 
            420                 425                 430         


Arg Leu Glu Asn Leu Ile Ala Gln Leu Pro Gly Glu Lys Lys Asn Gly 
        435                 440                 445             


Leu Phe Gly Asn Leu Ile Ala Leu Ser Leu Gly Leu Thr Pro Asn Phe 
    450                 455                 460                 


Lys Ser Asn Phe Asp Leu Ala Glu Asp Ala Lys Leu Gln Leu Ser Lys 
465                 470                 475                 480 


Asp Thr Tyr Asp Asp Asp Leu Asp Asn Leu Leu Ala Gln Ile Gly Asp 
                485                 490                 495     


Gln Tyr Ala Asp Leu Phe Leu Ala Ala Lys Asn Leu Ser Asp Ala Ile 
            500                 505                 510         


Leu Leu Ser Asp Ile Leu Arg Val Asn Thr Glu Ile Thr Lys Ala Pro 
        515                 520                 525             


Leu Ser Ala Ser Met Ile Lys Arg Tyr Asp Glu His His Gln Asp Leu 
    530                 535                 540                 


Thr Leu Leu Lys Ala Leu Val Arg Gln Gln Leu Pro Glu Lys Tyr Lys 
545                 550                 555                 560 


Glu Ile Phe Phe Asp Gln Ser Lys Asn Gly Tyr Ala Gly Tyr Ile Asp 
                565                 570                 575     


Gly Gly Ala Ser Gln Glu Glu Phe Tyr Lys Phe Ile Lys Pro Ile Leu 
            580                 585                 590         


Glu Lys Met Asp Gly Thr Glu Glu Leu Leu Val Lys Leu Asn Arg Glu 
        595                 600                 605             


Asp Leu Leu Arg Lys Gln Arg Thr Phe Asp Asn Gly Ser Ile Pro His 
    610                 615                 620                 


Gln Ile His Leu Gly Glu Leu His Ala Ile Leu Arg Arg Gln Glu Asp 
625                 630                 635                 640 


Phe Tyr Pro Phe Leu Lys Asp Asn Arg Glu Lys Ile Glu Lys Ile Leu 
                645                 650                 655     


Thr Phe Arg Ile Pro Tyr Tyr Val Gly Pro Leu Ala Arg Gly Asn Ser 
            660                 665                 670         


Arg Phe Ala Trp Met Thr Arg Lys Ser Glu Glu Thr Ile Thr Pro Trp 
        675                 680                 685             


Asn Phe Glu Glu Val Val Asp Lys Gly Ala Ser Ala Gln Ser Phe Ile 
    690                 695                 700                 


Glu Arg Met Thr Asn Phe Asp Lys Asn Leu Pro Asn Glu Lys Val Leu 
705                 710                 715                 720 


Pro Lys His Ser Leu Leu Tyr Glu Tyr Phe Thr Val Tyr Asn Glu Leu 
                725                 730                 735     


Thr Lys Val Lys Tyr Val Thr Glu Gly Met Arg Lys Pro Ala Phe Leu 
            740                 745                 750         


Ser Gly Glu Gln Lys Lys Ala Ile Val Asp Leu Leu Phe Lys Thr Asn 
        755                 760                 765             


Arg Lys Val Thr Val Lys Gln Leu Lys Glu Asp Tyr Phe Lys Lys Ile 
    770                 775                 780                 


Glu Cys Phe Asp Ser Val Glu Ile Ser Gly Val Glu Asp Arg Phe Asn 
785                 790                 795                 800 


Ala Ser Leu Gly Thr Tyr His Asp Leu Leu Lys Ile Ile Lys Asp Lys 
                805                 810                 815     


Asp Phe Leu Asp Asn Glu Glu Asn Glu Asp Ile Leu Glu Asp Ile Val 
            820                 825                 830         


Leu Thr Leu Thr Leu Phe Glu Asp Arg Glu Met Ile Glu Glu Arg Leu 
        835                 840                 845             


Lys Thr Tyr Ala His Leu Phe Asp Asp Lys Val Met Lys Gln Leu Lys 
    850                 855                 860                 


Arg Arg Arg Tyr Thr Gly Trp Gly Arg Leu Ser Arg Lys Leu Ile Asn 
865                 870                 875                 880 


Gly Ile Arg Asp Lys Gln Ser Gly Lys Thr Ile Leu Asp Phe Leu Lys 
                885                 890                 895     


Ser Asp Gly Phe Ala Asn Arg Asn Phe Met Gln Leu Ile His Asp Asp 
            900                 905                 910         


Ser Leu Thr Phe Lys Glu Asp Ile Gln Lys Ala Gln Val Ser Gly Gln 
        915                 920                 925             


Gly Asp Ser Leu His Glu His Ile Ala Asn Leu Ala Gly Ser Pro Ala 
    930                 935                 940                 


Ile Lys Lys Gly Ile Leu Gln Thr Val Lys Val Val Asp Glu Leu Val 
945                 950                 955                 960 


Lys Val Met Gly Arg His Lys Pro Glu Asn Ile Val Ile Glu Met Ala 
                965                 970                 975     


Arg Glu Asn Gln Thr Thr Gln Lys Gly Gln Lys Asn Ser Arg Glu Arg 
            980                 985                 990         


Met Lys Arg Ile Glu Glu Gly Ile  Lys Glu Leu Gly Ser  Gln Ile Leu 
        995                 1000                 1005             


Lys Glu  His Pro Val Glu Asn  Thr Gln Leu Gln Asn  Glu Lys Leu 
    1010                 1015                 1020             


Tyr Leu  Tyr Tyr Leu Gln Asn  Gly Arg Asp Met Tyr  Val Asp Gln 
    1025                 1030                 1035             


Glu Leu  Asp Ile Asn Arg Leu  Ser Asp Tyr Asp Val  Asp Ala Ile 
    1040                 1045                 1050             


Val Pro  Gln Ser Phe Leu Lys  Asp Asp Ser Ile Asp  Asn Lys Val 
    1055                 1060                 1065             


Leu Thr  Arg Ser Asp Lys Asn  Arg Gly Lys Ser Asp  Asn Val Pro 
    1070                 1075                 1080             


Ser Glu  Glu Val Val Lys Lys  Met Lys Asn Tyr Trp  Arg Gln Leu 
    1085                 1090                 1095             


Leu Asn  Ala Lys Leu Ile Thr  Gln Arg Lys Phe Asp  Asn Leu Thr 
    1100                 1105                 1110             


Lys Ala  Glu Arg Gly Gly Leu  Ser Glu Leu Asp Lys  Ala Gly Phe 
    1115                 1120                 1125             


Ile Lys  Arg Gln Leu Val Glu  Thr Arg Gln Ile Thr  Lys His Val 
    1130                 1135                 1140             


Ala Gln  Ile Leu Asp Ser Arg  Met Asn Thr Lys Tyr  Asp Glu Asn 
    1145                 1150                 1155             


Asp Lys  Leu Ile Arg Glu Val  Lys Val Ile Thr Leu  Lys Ser Lys 
    1160                 1165                 1170             


Leu Val  Ser Asp Phe Arg Lys  Asp Phe Gln Phe Tyr  Lys Val Arg 
    1175                 1180                 1185             


Glu Ile  Asn Asn Tyr His His  Ala His Asp Ala Tyr  Leu Asn Ala 
    1190                 1195                 1200             


Val Val  Gly Thr Ala Leu Ile  Lys Lys Tyr Pro Lys  Leu Glu Ser 
    1205                 1210                 1215             


Glu Phe  Val Tyr Gly Asp Tyr  Lys Val Tyr Asp Val  Arg Lys Met 
    1220                 1225                 1230             


Ile Ala  Lys Ser Glu Gln Glu  Ile Gly Lys Ala Thr  Ala Lys Tyr 
    1235                 1240                 1245             


Phe Phe  Tyr Ser Asn Ile Met  Asn Phe Phe Lys Thr  Glu Ile Thr 
    1250                 1255                 1260             


Leu Ala  Asn Gly Glu Ile Arg  Lys Arg Pro Leu Ile  Glu Thr Asn 
    1265                 1270                 1275             


Gly Glu  Thr Gly Glu Ile Val  Trp Asp Lys Gly Arg  Asp Phe Ala 
    1280                 1285                 1290             


Thr Val  Arg Lys Val Leu Ser  Met Pro Gln Val Asn  Ile Val Lys 
    1295                 1300                 1305             


Lys Thr  Glu Val Gln Thr Gly  Gly Phe Ser Lys Glu  Ser Ile Leu 
    1310                 1315                 1320             


Pro Lys  Arg Asn Ser Asp Lys  Leu Ile Ala Arg Lys  Lys Asp Trp 
    1325                 1330                 1335             


Asp Pro  Lys Lys Tyr Gly Gly  Phe Asp Ser Pro Thr  Val Ala Tyr 
    1340                 1345                 1350             


Ser Val  Leu Val Val Ala Lys  Val Glu Lys Gly Lys  Ser Lys Lys 
    1355                 1360                 1365             


Leu Lys  Ser Val Lys Glu Leu  Leu Gly Ile Thr Ile  Met Glu Arg 
    1370                 1375                 1380             


Ser Ser  Phe Glu Lys Asn Pro  Ile Asp Phe Leu Glu  Ala Lys Gly 
    1385                 1390                 1395             


Tyr Lys  Glu Val Lys Lys Asp  Leu Ile Ile Lys Leu  Pro Lys Tyr 
    1400                 1405                 1410             


Ser Leu  Phe Glu Leu Glu Asn  Gly Arg Lys Arg Met  Leu Ala Ser 
    1415                 1420                 1425             


Ala Gly  Glu Leu Gln Lys Gly  Asn Glu Leu Ala Leu  Pro Ser Lys 
    1430                 1435                 1440             


Tyr Val  Asn Phe Leu Tyr Leu  Ala Ser His Tyr Glu  Lys Leu Lys 
    1445                 1450                 1455             


Gly Ser  Pro Glu Asp Asn Glu  Gln Lys Gln Leu Phe  Val Glu Gln 
    1460                 1465                 1470             


His Lys  His Tyr Leu Asp Glu  Ile Ile Glu Gln Ile  Ser Glu Phe 
    1475                 1480                 1485             


Ser Lys  Arg Val Ile Leu Ala  Asp Ala Asn Leu Asp  Lys Val Leu 
    1490                 1495                 1500             


Ser Ala  Tyr Asn Lys His Arg  Asp Lys Pro Ile Arg  Glu Gln Ala 
    1505                 1510                 1515             


Glu Asn  Ile Ile His Leu Phe  Thr Leu Thr Asn Leu  Gly Ala Pro 
    1520                 1525                 1530             


Ala Ala  Phe Lys Tyr Phe Asp  Thr Thr Ile Asp Arg  Lys Arg Tyr 
    1535                 1540                 1545             


Thr Ser  Thr Lys Glu Val Leu  Asp Ala Thr Leu Ile  His Gln Ser 
    1550                 1555                 1560             


Ile Thr  Gly Leu Tyr Glu Thr  Arg Ile Asp Leu Ser  Gln Leu Gly 
    1565                 1570                 1575             


Gly Asp  Gly Ser Pro Lys Lys  Lys Arg Lys Val Ser  Ser 
    1580                 1585                 1590     


<210>  41
<211>  4073
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  pHR-GFP- selection gene plasmid

<400>  41
ggggccacta gggacaggat cggcgtcttc actcgctggg ttcccttttc cttctccttc       60

tggggcctgt gccatctctc gtttcttagg atggccttct ccgacggatg tctcccttgc      120

gtcccgcctc cccttcttgt aggcctgcat catcaccgtt tttctggaca accccaaagt      180

accccgtctc cctggcttta gccacctctc catcctcttg ctttctttgc ctggacaccc      240

cgttctcctg tggattcggg tcacctctca ctcctttcat ttgggcagct cccctacccc      300

ccttacctct ctagtctgtg ctagctcttc cagccccctg tcatggcatc ttccaggggt      360

ccgagagctc agctagtctt cttcctccaa cccgggcccc tatgtccact tcaggacagc      420

atgtttgctg cctccaggga tcctgtgtcc ccgagctggg accaccttat attcccaggg      480

ccggttaatg tggctctggt tctgggtact tttatctgtc ccgacgtccg atcgaaccat      540

ggacagttag ctttgcaaag atggataaag ttttaaacag agaggaatct ttgcagctaa      600

tggaccttct aggtcttgaa aggagtggga attggctccg gtgcccgtca gtgggcagag      660

cgcacatcgc ccacagtccc cgagaagttg gggggagggg tcggcaattg aaccggtgcc      720

tagagaaggt ggcgcggggt aaactgggaa agtgatgtcg tgtactggct ccgccttttt      780

cccgagggtg ggggagaacc gtatataagt gcagtagtcg ccgtgaacgt tctttttcgc      840

aacgggtttg ccgccagaac acaggtaagt gccgtgtgtg gttcccgcgg gcctggcctc      900

tttacgggtt atggcccttg cgtgccttga attacttcca cctggctgca gtacgtgatt      960

cttgatcccg agcttcgggt tggaagtggg tgggagagtt cgaggccttg cgcttaagga     1020

gccccttcgc ctcgtgcttg agttgaggcc tggcctgggc gctggggccg ccgcgtgcga     1080

atctggtggc accttcgcgc ctgtctcgct gctttcgata agtctctagc catttaaaat     1140

ttttgatgac ctgctgcgac gctttttttc tggcaagata gtcttgtaaa tgcgggccaa     1200

gatctgcaca ctggtatttc ggtttttggg gccgcgggcg gcgacggggc ccgtgcgtcc     1260

cagcgcacat gttcggcgag gcggggcctg cgagcgcggc caccgagaat cggacggggg     1320

tagtctcaag ctggccggcc tgctctggtg cctggcctcg cgccgccgtg tatcgccccg     1380

ccctgggcgg caaggctggc ccggtcggca ccagttgcgt gagcggaaag atggccgctt     1440

cccggccctg ctgcagggag ctcaaaatgg aggacgcggc gctcgggaga gcgggcgggt     1500

gagtcaccca cacaaaggaa aagggccttt ccgtcctcag ccgtcgcttc atgtgactcc     1560

acggagtacc gggcgccgtc caggcacctc gattagttct cgagcttttg gagtacgtcg     1620

tctttaggtt ggggggaggg gttttatgcg atggagtttc cccacactga gtgggtggag     1680

actgaagtta ggccagcttg gcacttgatg taattctcct tggaatttgc cctttttgag     1740

tttggatctt ggttcattct caagcctcag acagtggttc aaagtttttt tcttccattt     1800

caggtgtcgt gagaattcta atacgactca ctatagggtg tgctgtctca tcattttggc     1860

aaagattggc caccaagctt accgccatgg tgagcaaggg cgaggagctg ttcaccgggg     1920

tggtgcccat cctggtcgag ctggacggcg acgtaaacgg ccacaagttc agcgtgtccg     1980

gcgagggcga gggcgatgcc acctacggca agctgaccct gaagttcatc tgcaccaccg     2040

gcaagctgcc cgtgccctgg cccaccctcg tgaccaccct gacctacggc gtgcagtgct     2100

tcagccgcta ccccgaccac atgaagcagc acgacttctt caagtccgcc atgcccgaag     2160

gctacgtcca ggagcgcacc atcttcttca aggacgacgg caactacaag acccgcgccg     2220

aggtgaagtt cgagggcgac accctggtga accgcatcga gctgaagggc atcgacttca     2280

aggaggacgg caacatcctg gggcacaagc tggagtacaa ctacaacagc cacaacgtct     2340

atatcatggc cgacaagcag aagaacggca tcaaggtgaa cttcaagatc cgccacaaca     2400

tcgaggacgg cagcgtgcag ctcgccgacc actaccagca gaacaccccc atcggcgacg     2460

gccccgtgct gctgcccgac aaccactacc tgagcaccca gtccgccctg agcaaagacc     2520

ccaacgagaa gcgtgatcac atggtcctgc tggagttcgt gaccgccgcc gggatcactc     2580

tcggcatgga cgagctgtac aaggaaggaa gaggcagcct gctgacatgt ggcgacgtgg     2640

aggagaaccc tggcccaatg gtgggcagcc tgaattgtat cgtggccgtg tcccagaaca     2700

tgggcatcgg caagaatggc gattttcctt ggccccctct gagaaatgag tccagatact     2760

ttcagaggat gaccacaacc agctccgtgg agggcaagca gaacctggtc atcatgggca     2820

agaagacatg gttctctatc ccagagaaga accgccccct gaagggccgg atcaatctgg     2880

tgctgagcag ggagctgaag gagccacccc agggagcaca ctttctgtcc aggtctctgg     2940

acgatgccct gaagctgacc gagcagcctg agctggccaa caaggtggac atggtgtgga     3000

tcgtgggcgg ctctagcgtg tataaggagg ccatgaatca ccctggccac ctgaagctgt     3060

tcgtgacacg gatcatgcag gactttgagt ccgatacctt ctttccagag atcgacctgg     3120

agaagtacaa gctgctgccc gagtatcctg gcgtgctgtc tgatgtgcag gaggagaagg     3180

gcatcaagta caagttcgag gtgtatgaga agaacgattg ataacatatg cctttaatta     3240

aacactagtt ctatagtgtc acctaaattc cctttagtga gggttaatgg ccgtaggccg     3300

ccagaattgg gtccagacat gataagatac attgatgagt ttggacaaac cacaactaga     3360

atgcagtgaa aaaaatgctt tatttgtgaa atttgtgatg ctattgcttt atttgtaacc     3420

attataagct gcaataaaca agttaacaac aacaattgca ttcattttat gtttcaggtt     3480

cagggggagg tgtgggaggt tttttcggac tctaggacct gcgcatgcgc ttggggtacc     3540

taggatatcg acagaaaagc cccatcctta ggcctcctcc ttcctagtct cctgatattg     3600

ggtctaaccc ccacctcctg ttaggcagat tccttatctg gtgacacacc cccatttcct     3660

ggagccatct ctctccttgc cagaacctct aaggtttgct tacgatggag ccagagagga     3720

tcctgggagg gagagcttgg cagggggtgg gagggaaggg ggggatgcgt gacctgcccg     3780

gttctcagtg gccaccctgc gctaccctct cccagaacct gagctgctct gacgcggccg     3840

tctggtgcgt ttcactgatc ctggtgctgc agcttcctta cacttcccaa gaggagaagc     3900

agtttggaaa aacaaaatca gaataagttg gtcctgagtt ctaactttgg ctcttcacct     3960

ttctagtccc caatttatat tgttcctccg tgcgtcagtt ttacctgtga gataaggcca     4020

gtagccagcc ccgtcctggc agggctgtgc ctctagggac aggattggtg acg            4073


<210>  42
<211>  3089
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  pMMEJ-GFP-selection gene plasmid

<400>  42
ccaatcctgt ccctagtggc cccactgtgg ggacgtccga tcgaaccatg gacagttagc       60

tttgcaaaga tggataaagt tttaaacaga gaggaatctt tgcagctaat ggaccttcta      120

ggtcttgaaa ggagtgggaa ttggctccgg tgcccgtcag tgggcagagc gcacatcgcc      180

cacagtcccc gagaagttgg ggggaggggt cggcaattga accggtgcct agagaaggtg      240

gcgcggggta aactgggaaa gtgatgtcgt gtactggctc cgcctttttc ccgagggtgg      300

gggagaaccg tatataagtg cagtagtcgc cgtgaacgtt ctttttcgca acgggtttgc      360

cgccagaaca caggtaagtg ccgtgtgtgg ttcccgcggg cctggcctct ttacgggtta      420

tggcccttgc gtgccttgaa ttacttccac ctggctgcag tacgtgattc ttgatcccga      480

gcttcgggtt ggaagtgggt gggagagttc gaggccttgc gcttaaggag ccccttcgcc      540

tcgtgcttga gttgaggcct ggcctgggcg ctggggccgc cgcgtgcgaa tctggtggca      600

ccttcgcgcc tgtctcgctg ctttcgataa gtctctagcc atttaaaatt tttgatgacc      660

tgctgcgacg ctttttttct ggcaagatag tcttgtaaat gcgggccaag atctgcacac      720

tggtatttcg gtttttgggg ccgcgggcgg cgacggggcc cgtgcgtccc agcgcacatg      780

ttcggcgagg cggggcctgc gagcgcggcc accgagaatc ggacgggggt agtctcaagc      840

tggccggcct gctctggtgc ctggcctcgc gccgccgtgt atcgccccgc cctgggcggc      900

aaggctggcc cggtcggcac cagttgcgtg agcggaaaga tggccgcttc ccggccctgc      960

tgcagggagc tcaaaatgga ggacgcggcg ctcgggagag cgggcgggtg agtcacccac     1020

acaaaggaaa agggcctttc cgtcctcagc cgtcgcttca tgtgactcca cggagtaccg     1080

ggcgccgtcc aggcacctcg attagttctc gagcttttgg agtacgtcgt ctttaggttg     1140

gggggagggg ttttatgcga tggagtttcc ccacactgag tgggtggaga ctgaagttag     1200

gccagcttgg cacttgatgt aattctcctt ggaatttgcc ctttttgagt ttggatcttg     1260

gttcattctc aagcctcaga cagtggttca aagttttttt cttccatttc aggtgtcgtg     1320

agaattctaa tacgactcac tatagggtgt gctgtctcat cattttggca aagattggcc     1380

accaagctta ccgccatggt gagcaagggc gaggagctgt tcaccggggt ggtgcccatc     1440

ctggtcgagc tggacggcga cgtaaacggc cacaagttca gcgtgtccgg cgagggcgag     1500

ggcgatgcca cctacggcaa gctgaccctg aagttcatct gcaccaccgg caagctgccc     1560

gtgccctggc ccaccctcgt gaccaccctg acctacggcg tgcagtgctt cagccgctac     1620

cccgaccaca tgaagcagca cgacttcttc aagtccgcca tgcccgaagg ctacgtccag     1680

gagcgcacca tcttcttcaa ggacgacggc aactacaaga cccgcgccga ggtgaagttc     1740

gagggcgaca ccctggtgaa ccgcatcgag ctgaagggca tcgacttcaa ggaggacggc     1800

aacatcctgg ggcacaagct ggagtacaac tacaacagcc acaacgtcta tatcatggcc     1860

gacaagcaga agaacggcat caaggtgaac ttcaagatcc gccacaacat cgaggacggc     1920

agcgtgcagc tcgccgacca ctaccagcag aacaccccca tcggcgacgg ccccgtgctg     1980

ctgcccgaca accactacct gagcacccag tccgccctga gcaaagaccc caacgagaag     2040

cgtgatcaca tggtcctgct ggagttcgtg accgccgccg ggatcactct cggcatggac     2100

gagctgtaca aggaaggaag aggcagcctg ctgacatgtg gcgacgtgga ggagaaccct     2160

ggcccaatgg tgggcagcct gaattgtatc gtggccgtgt cccagaacat gggcatcggc     2220

aagaatggcg attttccttg gccccctctg agaaatgagt ccagatactt tcagaggatg     2280

accacaacca gctccgtgga gggcaagcag aacctggtca tcatgggcaa gaagacatgg     2340

ttctctatcc cagagaagaa ccgccccctg aagggccgga tcaatctggt gctgagcagg     2400

gagctgaagg agccacccca gggagcacac tttctgtcca ggtctctgga cgatgccctg     2460

aagctgaccg agcagcctga gctggccaac aaggtggaca tggtgtggat cgtgggcggc     2520

tctagcgtgt ataaggaggc catgaatcac cctggccacc tgaagctgtt cgtgacacgg     2580

atcatgcagg actttgagtc cgataccttc tttccagaga tcgacctgga gaagtacaag     2640

ctgctgcccg agtatcctgg cgtgctgtct gatgtgcagg aggagaaggg catcaagtac     2700

aagttcgagg tgtatgagaa gaacgattga taacatatgc ctttaattaa acactagttc     2760

tatagtgtca cctaaattcc ctttagtgag ggttaatggc cgtaggccgc cagaattggg     2820

tccagacatg ataagataca ttgatgagtt tggacaaacc acaactagaa tgcagtgaaa     2880

aaaatgcttt atttgtgaaa tttgtgatgc tattgcttta tttgtaacca ttataagctg     2940

caataaacaa gttaacaaca acaattgcat tcattttatg tttcaggttc agggggaggt     3000

gtgggaggtt ttttcggact ctaggacctg cgcatgcgct tggggtacct aggatatcgg     3060

atggggcttt tctgtcacca atcctgagg                                       3089


