                         SEQUENCE LISTING

<110>  The Dow Chemical Company
       Sanghani, Paresh
       Greenwalt, Scott
       Bhosale, Prakash
       Shiue, Eric
 
<120>  Process to Prepare Elongated 2-Ketoacids and C5-C10 Compounds 
       Therefrom via Genetic Modifications to Microbial Metabolic 
       Pathways

<130>  DOW 79046 MA/79046.369

<160>  37    

<170>  PatentIn version 3.5

<210>  1
<211>  466
<212>  PRT
<213>  Escherichia coli

<400>  1

Met Ala Lys Thr Leu Tyr Glu Lys Leu Phe Asp Ala His Val Val Tyr 
1               5                   10                  15      


Glu Ala Glu Asn Glu Thr Pro Leu Leu Tyr Ile Asp Arg His Leu Val 
            20                  25                  30          


His Glu Val Thr Ser Pro Gln Ala Phe Asp Gly Leu Arg Ala His Gly 
        35                  40                  45              


Arg Pro Val Arg Gln Pro Gly Lys Thr Phe Ala Thr Met Asp His Asn 
    50                  55                  60                  


Val Ser Thr Gln Thr Lys Asp Ile Asn Ala Cys Gly Glu Met Ala Arg 
65                  70                  75                  80  


Ile Gln Met Gln Glu Leu Ile Lys Asn Cys Lys Glu Phe Gly Val Glu 
                85                  90                  95      


Leu Tyr Asp Leu Asn His Pro Tyr Gln Gly Ile Val His Val Met Gly 
            100                 105                 110         


Pro Glu Gln Gly Val Thr Leu Pro Gly Met Thr Ile Val Cys Gly Asp 
        115                 120                 125             


Ser His Thr Ala Thr His Gly Ala Phe Gly Ala Leu Ala Phe Gly Ile 
    130                 135                 140                 


Gly Thr Ser Glu Val Glu His Val Leu Ala Thr Gln Thr Leu Lys Gln 
145                 150                 155                 160 


Gly Arg Ala Lys Thr Met Lys Ile Glu Val Gln Gly Lys Ala Ala Pro 
                165                 170                 175     


Gly Ile Thr Ala Lys Asp Ile Val Leu Ala Ile Ile Gly Lys Thr Gly 
            180                 185                 190         


Ser Ala Gly Gly Thr Gly His Val Val Glu Phe Cys Gly Glu Ala Ile 
        195                 200                 205             


Arg Asp Leu Ser Met Glu Gly Arg Met Thr Leu Cys Asn Met Ala Ile 
    210                 215                 220                 


Glu Met Gly Ala Lys Ala Gly Leu Val Ala Pro Asp Glu Thr Thr Phe 
225                 230                 235                 240 


Asn Tyr Val Lys Gly Arg Leu His Ala Pro Lys Gly Lys Asp Phe Asp 
                245                 250                 255     


Asp Ala Val Ala Tyr Trp Lys Thr Leu Gln Thr Asp Glu Gly Ala Thr 
            260                 265                 270         


Phe Asp Thr Val Val Thr Leu Gln Ala Glu Glu Ile Ser Pro Gln Val 
        275                 280                 285             


Thr Trp Gly Thr Asn Pro Gly Gln Val Ile Ser Val Asn Asp Asn Ile 
    290                 295                 300                 


Pro Asp Pro Ala Ser Phe Ala Asp Pro Val Glu Arg Ala Ser Ala Glu 
305                 310                 315                 320 


Lys Ala Leu Ala Tyr Met Gly Leu Lys Pro Gly Ile Pro Leu Thr Glu 
                325                 330                 335     


Val Ala Ile Asp Lys Val Phe Ile Gly Ser Cys Thr Asn Ser Arg Ile 
            340                 345                 350         


Glu Asp Leu Arg Ala Ala Ala Glu Ile Ala Lys Gly Arg Lys Val Ala 
        355                 360                 365             


Pro Gly Val Gln Ala Leu Val Val Pro Gly Ser Gly Pro Val Lys Ala 
    370                 375                 380                 


Gln Ala Glu Ala Glu Gly Leu Asp Lys Ile Phe Ile Glu Ala Gly Phe 
385                 390                 395                 400 


Glu Trp Arg Leu Pro Gly Cys Ser Met Cys Leu Ala Met Asn Asn Asp 
                405                 410                 415     


Arg Leu Asn Pro Gly Glu Arg Cys Ala Ser Thr Ser Asn Arg Asn Phe 
            420                 425                 430         


Glu Gly Arg Gln Gly Arg Gly Gly Arg Thr His Leu Val Ser Pro Ala 
        435                 440                 445             


Met Ala Ala Ala Ala Ala Val Thr Gly His Phe Ala Asp Ile Arg Asn 
    450                 455                 460                 


Ile Lys 
465     


<210>  2
<211>  201
<212>  PRT
<213>  Escherichia coli

<400>  2

Met Ala Glu Lys Phe Ile Lys His Thr Gly Leu Val Val Pro Leu Asp 
1               5                   10                  15      


Ala Ala Asn Val Asp Thr Asp Ala Ile Ile Pro Lys Gln Phe Leu Gln 
            20                  25                  30          


Lys Val Thr Arg Thr Gly Phe Gly Ala His Leu Phe Asn Asp Trp Arg 
        35                  40                  45              


Phe Leu Asp Glu Lys Gly Gln Gln Pro Asn Pro Asp Phe Val Leu Asn 
    50                  55                  60                  


Phe Pro Gln Tyr Gln Gly Ala Ser Ile Leu Leu Ala Arg Glu Asn Phe 
65                  70                  75                  80  


Gly Cys Gly Ser Ser Arg Glu His Ala Pro Trp Ala Leu Thr Asp Tyr 
                85                  90                  95      


Gly Phe Lys Val Val Ile Ala Pro Ser Phe Ala Asp Ile Phe Tyr Gly 
            100                 105                 110         


Asn Ser Phe Asn Asn Gln Leu Leu Pro Val Lys Leu Ser Asp Ala Glu 
        115                 120                 125             


Val Asp Glu Leu Phe Ala Leu Val Lys Ala Asn Pro Gly Ile His Phe 
    130                 135                 140                 


Asp Val Asp Leu Glu Ala Gln Glu Val Lys Ala Gly Glu Lys Thr Tyr 
145                 150                 155                 160 


Arg Phe Thr Ile Asp Ala Phe Arg Arg His Cys Met Met Asn Gly Leu 
                165                 170                 175     


Asp Ser Ile Gly Leu Thr Leu Gln His Asp Asp Ala Ile Ala Ala Tyr 
            180                 185                 190         


Glu Ala Lys Gln Pro Ala Phe Met Asn 
        195                 200     


<210>  3
<211>  466
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthesized - Modified LeuC (V35A)

<400>  3

Met Ala Lys Thr Leu Tyr Glu Lys Leu Phe Asp Ala His Val Val Tyr 
1               5                   10                  15      


Glu Ala Glu Asn Glu Thr Pro Leu Leu Tyr Ile Asp Arg His Leu Val 
            20                  25                  30          


His Glu Ala Thr Ser Pro Gln Ala Phe Asp Gly Leu Arg Ala His Gly 
        35                  40                  45              


Arg Pro Val Arg Gln Pro Gly Lys Thr Phe Ala Thr Met Asp His Asn 
    50                  55                  60                  


Val Ser Thr Gln Thr Lys Asp Ile Asn Ala Cys Gly Glu Met Ala Arg 
65                  70                  75                  80  


Ile Gln Met Gln Glu Leu Ile Lys Asn Cys Lys Glu Phe Gly Val Glu 
                85                  90                  95      


Leu Tyr Asp Leu Asn His Pro Tyr Gln Gly Ile Val His Val Met Gly 
            100                 105                 110         


Pro Glu Gln Gly Val Thr Leu Pro Gly Met Thr Ile Val Cys Gly Asp 
        115                 120                 125             


Ser His Thr Ala Thr His Gly Ala Phe Gly Ala Leu Ala Phe Gly Ile 
    130                 135                 140                 


Gly Thr Ser Glu Val Glu His Val Leu Ala Thr Gln Thr Leu Lys Gln 
145                 150                 155                 160 


Gly Arg Ala Lys Thr Met Lys Ile Glu Val Gln Gly Lys Ala Ala Pro 
                165                 170                 175     


Gly Ile Thr Ala Lys Asp Ile Val Leu Ala Ile Ile Gly Lys Thr Gly 
            180                 185                 190         


Ser Ala Gly Gly Thr Gly His Val Val Glu Phe Cys Gly Glu Ala Ile 
        195                 200                 205             


Arg Asp Leu Ser Met Glu Gly Arg Met Thr Leu Cys Asn Met Ala Ile 
    210                 215                 220                 


Glu Met Gly Ala Lys Ala Gly Leu Val Ala Pro Asp Glu Thr Thr Phe 
225                 230                 235                 240 


Asn Tyr Val Lys Gly Arg Leu His Ala Pro Lys Gly Lys Asp Phe Asp 
                245                 250                 255     


Asp Ala Val Ala Tyr Trp Lys Thr Leu Gln Thr Asp Glu Gly Ala Thr 
            260                 265                 270         


Phe Asp Thr Val Val Thr Leu Gln Ala Glu Glu Ile Ser Pro Gln Val 
        275                 280                 285             


Thr Trp Gly Thr Asn Pro Gly Gln Val Ile Ser Val Asn Asp Asn Ile 
    290                 295                 300                 


Pro Asp Pro Ala Ser Phe Ala Asp Pro Val Glu Arg Ala Ser Ala Glu 
305                 310                 315                 320 


Lys Ala Leu Ala Tyr Met Gly Leu Lys Pro Gly Ile Pro Leu Thr Glu 
                325                 330                 335     


Val Ala Ile Asp Lys Val Phe Ile Gly Ser Cys Thr Asn Ser Arg Ile 
            340                 345                 350         


Glu Asp Leu Arg Ala Ala Ala Glu Ile Ala Lys Gly Arg Lys Val Ala 
        355                 360                 365             


Pro Gly Val Gln Ala Leu Val Val Pro Gly Ser Gly Pro Val Lys Ala 
    370                 375                 380                 


Gln Ala Glu Ala Glu Gly Leu Asp Lys Ile Phe Ile Glu Ala Gly Phe 
385                 390                 395                 400 


Glu Trp Arg Leu Pro Gly Cys Ser Met Cys Leu Ala Met Asn Asn Asp 
                405                 410                 415     


Arg Leu Asn Pro Gly Glu Arg Cys Ala Ser Thr Ser Asn Arg Asn Phe 
            420                 425                 430         


Glu Gly Arg Gln Gly Arg Gly Gly Arg Thr His Leu Val Ser Pro Ala 
        435                 440                 445             


Met Ala Ala Ala Ala Ala Val Thr Gly His Phe Ala Asp Ile Arg Asn 
    450                 455                 460                 


Ile Lys 
465     


<210>  4
<211>  466
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthesized - Modified LeuC (V35G)

<400>  4

Met Ala Lys Thr Leu Tyr Glu Lys Leu Phe Asp Ala His Val Val Tyr 
1               5                   10                  15      


Glu Ala Glu Asn Glu Thr Pro Leu Leu Tyr Ile Asp Arg His Leu Val 
            20                  25                  30          


His Glu Gly Thr Ser Pro Gln Ala Phe Asp Gly Leu Arg Ala His Gly 
        35                  40                  45              


Arg Pro Val Arg Gln Pro Gly Lys Thr Phe Ala Thr Met Asp His Asn 
    50                  55                  60                  


Val Ser Thr Gln Thr Lys Asp Ile Asn Ala Cys Gly Glu Met Ala Arg 
65                  70                  75                  80  


Ile Gln Met Gln Glu Leu Ile Lys Asn Cys Lys Glu Phe Gly Val Glu 
                85                  90                  95      


Leu Tyr Asp Leu Asn His Pro Tyr Gln Gly Ile Val His Val Met Gly 
            100                 105                 110         


Pro Glu Gln Gly Val Thr Leu Pro Gly Met Thr Ile Val Cys Gly Asp 
        115                 120                 125             


Ser His Thr Ala Thr His Gly Ala Phe Gly Ala Leu Ala Phe Gly Ile 
    130                 135                 140                 


Gly Thr Ser Glu Val Glu His Val Leu Ala Thr Gln Thr Leu Lys Gln 
145                 150                 155                 160 


Gly Arg Ala Lys Thr Met Lys Ile Glu Val Gln Gly Lys Ala Ala Pro 
                165                 170                 175     


Gly Ile Thr Ala Lys Asp Ile Val Leu Ala Ile Ile Gly Lys Thr Gly 
            180                 185                 190         


Ser Ala Gly Gly Thr Gly His Val Val Glu Phe Cys Gly Glu Ala Ile 
        195                 200                 205             


Arg Asp Leu Ser Met Glu Gly Arg Met Thr Leu Cys Asn Met Ala Ile 
    210                 215                 220                 


Glu Met Gly Ala Lys Ala Gly Leu Val Ala Pro Asp Glu Thr Thr Phe 
225                 230                 235                 240 


Asn Tyr Val Lys Gly Arg Leu His Ala Pro Lys Gly Lys Asp Phe Asp 
                245                 250                 255     


Asp Ala Val Ala Tyr Trp Lys Thr Leu Gln Thr Asp Glu Gly Ala Thr 
            260                 265                 270         


Phe Asp Thr Val Val Thr Leu Gln Ala Glu Glu Ile Ser Pro Gln Val 
        275                 280                 285             


Thr Trp Gly Thr Asn Pro Gly Gln Val Ile Ser Val Asn Asp Asn Ile 
    290                 295                 300                 


Pro Asp Pro Ala Ser Phe Ala Asp Pro Val Glu Arg Ala Ser Ala Glu 
305                 310                 315                 320 


Lys Ala Leu Ala Tyr Met Gly Leu Lys Pro Gly Ile Pro Leu Thr Glu 
                325                 330                 335     


Val Ala Ile Asp Lys Val Phe Ile Gly Ser Cys Thr Asn Ser Arg Ile 
            340                 345                 350         


Glu Asp Leu Arg Ala Ala Ala Glu Ile Ala Lys Gly Arg Lys Val Ala 
        355                 360                 365             


Pro Gly Val Gln Ala Leu Val Val Pro Gly Ser Gly Pro Val Lys Ala 
    370                 375                 380                 


Gln Ala Glu Ala Glu Gly Leu Asp Lys Ile Phe Ile Glu Ala Gly Phe 
385                 390                 395                 400 


Glu Trp Arg Leu Pro Gly Cys Ser Met Cys Leu Ala Met Asn Asn Asp 
                405                 410                 415     


Arg Leu Asn Pro Gly Glu Arg Cys Ala Ser Thr Ser Asn Arg Asn Phe 
            420                 425                 430         


Glu Gly Arg Gln Gly Arg Gly Gly Arg Thr His Leu Val Ser Pro Ala 
        435                 440                 445             


Met Ala Ala Ala Ala Ala Val Thr Gly His Phe Ala Asp Ile Arg Asn 
    450                 455                 460                 


Ile Lys 
465     


<210>  5
<211>  466
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthezied - Modified LeuC (L411A)

<400>  5

Met Ala Lys Thr Leu Tyr Glu Lys Leu Phe Asp Ala His Val Val Tyr 
1               5                   10                  15      


Glu Ala Glu Asn Glu Thr Pro Leu Leu Tyr Ile Asp Arg His Leu Val 
            20                  25                  30          


His Glu Val Thr Ser Pro Gln Ala Phe Asp Gly Leu Arg Ala His Gly 
        35                  40                  45              


Arg Pro Val Arg Gln Pro Gly Lys Thr Phe Ala Thr Met Asp His Asn 
    50                  55                  60                  


Val Ser Thr Gln Thr Lys Asp Ile Asn Ala Cys Gly Glu Met Ala Arg 
65                  70                  75                  80  


Ile Gln Met Gln Glu Leu Ile Lys Asn Cys Lys Glu Phe Gly Val Glu 
                85                  90                  95      


Leu Tyr Asp Leu Asn His Pro Tyr Gln Gly Ile Val His Val Met Gly 
            100                 105                 110         


Pro Glu Gln Gly Val Thr Leu Pro Gly Met Thr Ile Val Cys Gly Asp 
        115                 120                 125             


Ser His Thr Ala Thr His Gly Ala Phe Gly Ala Leu Ala Phe Gly Ile 
    130                 135                 140                 


Gly Thr Ser Glu Val Glu His Val Leu Ala Thr Gln Thr Leu Lys Gln 
145                 150                 155                 160 


Gly Arg Ala Lys Thr Met Lys Ile Glu Val Gln Gly Lys Ala Ala Pro 
                165                 170                 175     


Gly Ile Thr Ala Lys Asp Ile Val Leu Ala Ile Ile Gly Lys Thr Gly 
            180                 185                 190         


Ser Ala Gly Gly Thr Gly His Val Val Glu Phe Cys Gly Glu Ala Ile 
        195                 200                 205             


Arg Asp Leu Ser Met Glu Gly Arg Met Thr Leu Cys Asn Met Ala Ile 
    210                 215                 220                 


Glu Met Gly Ala Lys Ala Gly Leu Val Ala Pro Asp Glu Thr Thr Phe 
225                 230                 235                 240 


Asn Tyr Val Lys Gly Arg Leu His Ala Pro Lys Gly Lys Asp Phe Asp 
                245                 250                 255     


Asp Ala Val Ala Tyr Trp Lys Thr Leu Gln Thr Asp Glu Gly Ala Thr 
            260                 265                 270         


Phe Asp Thr Val Val Thr Leu Gln Ala Glu Glu Ile Ser Pro Gln Val 
        275                 280                 285             


Thr Trp Gly Thr Asn Pro Gly Gln Val Ile Ser Val Asn Asp Asn Ile 
    290                 295                 300                 


Pro Asp Pro Ala Ser Phe Ala Asp Pro Val Glu Arg Ala Ser Ala Glu 
305                 310                 315                 320 


Lys Ala Leu Ala Tyr Met Gly Leu Lys Pro Gly Ile Pro Leu Thr Glu 
                325                 330                 335     


Val Ala Ile Asp Lys Val Phe Ile Gly Ser Cys Thr Asn Ser Arg Ile 
            340                 345                 350         


Glu Asp Leu Arg Ala Ala Ala Glu Ile Ala Lys Gly Arg Lys Val Ala 
        355                 360                 365             


Pro Gly Val Gln Ala Leu Val Val Pro Gly Ser Gly Pro Val Lys Ala 
    370                 375                 380                 


Gln Ala Glu Ala Glu Gly Leu Asp Lys Ile Phe Ile Glu Ala Gly Phe 
385                 390                 395                 400 


Glu Trp Arg Leu Pro Gly Cys Ser Met Cys Ala Ala Met Asn Asn Asp 
                405                 410                 415     


Arg Leu Asn Pro Gly Glu Arg Cys Ala Ser Thr Ser Asn Arg Asn Phe 
            420                 425                 430         


Glu Gly Arg Gln Gly Arg Gly Gly Arg Thr His Leu Val Ser Pro Ala 
        435                 440                 445             


Met Ala Ala Ala Ala Ala Val Thr Gly His Phe Ala Asp Ile Arg Asn 
    450                 455                 460                 


Ile Lys 
465     


<210>  6
<211>  466
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthesized - Modified LeuC (L411G)

<400>  6

Met Ala Lys Thr Leu Tyr Glu Lys Leu Phe Asp Ala His Val Val Tyr 
1               5                   10                  15      


Glu Ala Glu Asn Glu Thr Pro Leu Leu Tyr Ile Asp Arg His Leu Val 
            20                  25                  30          


His Glu Val Thr Ser Pro Gln Ala Phe Asp Gly Leu Arg Ala His Gly 
        35                  40                  45              


Arg Pro Val Arg Gln Pro Gly Lys Thr Phe Ala Thr Met Asp His Asn 
    50                  55                  60                  


Val Ser Thr Gln Thr Lys Asp Ile Asn Ala Cys Gly Glu Met Ala Arg 
65                  70                  75                  80  


Ile Gln Met Gln Glu Leu Ile Lys Asn Cys Lys Glu Phe Gly Val Glu 
                85                  90                  95      


Leu Tyr Asp Leu Asn His Pro Tyr Gln Gly Ile Val His Val Met Gly 
            100                 105                 110         


Pro Glu Gln Gly Val Thr Leu Pro Gly Met Thr Ile Val Cys Gly Asp 
        115                 120                 125             


Ser His Thr Ala Thr His Gly Ala Phe Gly Ala Leu Ala Phe Gly Ile 
    130                 135                 140                 


Gly Thr Ser Glu Val Glu His Val Leu Ala Thr Gln Thr Leu Lys Gln 
145                 150                 155                 160 


Gly Arg Ala Lys Thr Met Lys Ile Glu Val Gln Gly Lys Ala Ala Pro 
                165                 170                 175     


Gly Ile Thr Ala Lys Asp Ile Val Leu Ala Ile Ile Gly Lys Thr Gly 
            180                 185                 190         


Ser Ala Gly Gly Thr Gly His Val Val Glu Phe Cys Gly Glu Ala Ile 
        195                 200                 205             


Arg Asp Leu Ser Met Glu Gly Arg Met Thr Leu Cys Asn Met Ala Ile 
    210                 215                 220                 


Glu Met Gly Ala Lys Ala Gly Leu Val Ala Pro Asp Glu Thr Thr Phe 
225                 230                 235                 240 


Asn Tyr Val Lys Gly Arg Leu His Ala Pro Lys Gly Lys Asp Phe Asp 
                245                 250                 255     


Asp Ala Val Ala Tyr Trp Lys Thr Leu Gln Thr Asp Glu Gly Ala Thr 
            260                 265                 270         


Phe Asp Thr Val Val Thr Leu Gln Ala Glu Glu Ile Ser Pro Gln Val 
        275                 280                 285             


Thr Trp Gly Thr Asn Pro Gly Gln Val Ile Ser Val Asn Asp Asn Ile 
    290                 295                 300                 


Pro Asp Pro Ala Ser Phe Ala Asp Pro Val Glu Arg Ala Ser Ala Glu 
305                 310                 315                 320 


Lys Ala Leu Ala Tyr Met Gly Leu Lys Pro Gly Ile Pro Leu Thr Glu 
                325                 330                 335     


Val Ala Ile Asp Lys Val Phe Ile Gly Ser Cys Thr Asn Ser Arg Ile 
            340                 345                 350         


Glu Asp Leu Arg Ala Ala Ala Glu Ile Ala Lys Gly Arg Lys Val Ala 
        355                 360                 365             


Pro Gly Val Gln Ala Leu Val Val Pro Gly Ser Gly Pro Val Lys Ala 
    370                 375                 380                 


Gln Ala Glu Ala Glu Gly Leu Asp Lys Ile Phe Ile Glu Ala Gly Phe 
385                 390                 395                 400 


Glu Trp Arg Leu Pro Gly Cys Ser Met Cys Gly Ala Met Asn Asn Asp 
                405                 410                 415     


Arg Leu Asn Pro Gly Glu Arg Cys Ala Ser Thr Ser Asn Arg Asn Phe 
            420                 425                 430         


Glu Gly Arg Gln Gly Arg Gly Gly Arg Thr His Leu Val Ser Pro Ala 
        435                 440                 445             


Met Ala Ala Ala Ala Ala Val Thr Gly His Phe Ala Asp Ile Arg Asn 
    450                 455                 460                 


Ile Lys 
465     


<210>  7
<211>  466
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthesized - Modified LeuC (L411V)

<400>  7

Met Ala Lys Thr Leu Tyr Glu Lys Leu Phe Asp Ala His Val Val Tyr 
1               5                   10                  15      


Glu Ala Glu Asn Glu Thr Pro Leu Leu Tyr Ile Asp Arg His Leu Val 
            20                  25                  30          


His Glu Val Thr Ser Pro Gln Ala Phe Asp Gly Leu Arg Ala His Gly 
        35                  40                  45              


Arg Pro Val Arg Gln Pro Gly Lys Thr Phe Ala Thr Met Asp His Asn 
    50                  55                  60                  


Val Ser Thr Gln Thr Lys Asp Ile Asn Ala Cys Gly Glu Met Ala Arg 
65                  70                  75                  80  


Ile Gln Met Gln Glu Leu Ile Lys Asn Cys Lys Glu Phe Gly Val Glu 
                85                  90                  95      


Leu Tyr Asp Leu Asn His Pro Tyr Gln Gly Ile Val His Val Met Gly 
            100                 105                 110         


Pro Glu Gln Gly Val Thr Leu Pro Gly Met Thr Ile Val Cys Gly Asp 
        115                 120                 125             


Ser His Thr Ala Thr His Gly Ala Phe Gly Ala Leu Ala Phe Gly Ile 
    130                 135                 140                 


Gly Thr Ser Glu Val Glu His Val Leu Ala Thr Gln Thr Leu Lys Gln 
145                 150                 155                 160 


Gly Arg Ala Lys Thr Met Lys Ile Glu Val Gln Gly Lys Ala Ala Pro 
                165                 170                 175     


Gly Ile Thr Ala Lys Asp Ile Val Leu Ala Ile Ile Gly Lys Thr Gly 
            180                 185                 190         


Ser Ala Gly Gly Thr Gly His Val Val Glu Phe Cys Gly Glu Ala Ile 
        195                 200                 205             


Arg Asp Leu Ser Met Glu Gly Arg Met Thr Leu Cys Asn Met Ala Ile 
    210                 215                 220                 


Glu Met Gly Ala Lys Ala Gly Leu Val Ala Pro Asp Glu Thr Thr Phe 
225                 230                 235                 240 


Asn Tyr Val Lys Gly Arg Leu His Ala Pro Lys Gly Lys Asp Phe Asp 
                245                 250                 255     


Asp Ala Val Ala Tyr Trp Lys Thr Leu Gln Thr Asp Glu Gly Ala Thr 
            260                 265                 270         


Phe Asp Thr Val Val Thr Leu Gln Ala Glu Glu Ile Ser Pro Gln Val 
        275                 280                 285             


Thr Trp Gly Thr Asn Pro Gly Gln Val Ile Ser Val Asn Asp Asn Ile 
    290                 295                 300                 


Pro Asp Pro Ala Ser Phe Ala Asp Pro Val Glu Arg Ala Ser Ala Glu 
305                 310                 315                 320 


Lys Ala Leu Ala Tyr Met Gly Leu Lys Pro Gly Ile Pro Leu Thr Glu 
                325                 330                 335     


Val Ala Ile Asp Lys Val Phe Ile Gly Ser Cys Thr Asn Ser Arg Ile 
            340                 345                 350         


Glu Asp Leu Arg Ala Ala Ala Glu Ile Ala Lys Gly Arg Lys Val Ala 
        355                 360                 365             


Pro Gly Val Gln Ala Leu Val Val Pro Gly Ser Gly Pro Val Lys Ala 
    370                 375                 380                 


Gln Ala Glu Ala Glu Gly Leu Asp Lys Ile Phe Ile Glu Ala Gly Phe 
385                 390                 395                 400 


Glu Trp Arg Leu Pro Gly Cys Ser Met Cys Val Ala Met Asn Asn Asp 
                405                 410                 415     


Arg Leu Asn Pro Gly Glu Arg Cys Ala Ser Thr Ser Asn Arg Asn Phe 
            420                 425                 430         


Glu Gly Arg Gln Gly Arg Gly Gly Arg Thr His Leu Val Ser Pro Ala 
        435                 440                 445             


Met Ala Ala Ala Ala Ala Val Thr Gly His Phe Ala Asp Ile Arg Asn 
    450                 455                 460                 


Ile Lys 
465     


<210>  8
<211>  466
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthesized - Modified LeuC (V35A L411A)

<400>  8

Met Ala Lys Thr Leu Tyr Glu Lys Leu Phe Asp Ala His Val Val Tyr 
1               5                   10                  15      


Glu Ala Glu Asn Glu Thr Pro Leu Leu Tyr Ile Asp Arg His Leu Val 
            20                  25                  30          


His Glu Ala Thr Ser Pro Gln Ala Phe Asp Gly Leu Arg Ala His Gly 
        35                  40                  45              


Arg Pro Val Arg Gln Pro Gly Lys Thr Phe Ala Thr Met Asp His Asn 
    50                  55                  60                  


Val Ser Thr Gln Thr Lys Asp Ile Asn Ala Cys Gly Glu Met Ala Arg 
65                  70                  75                  80  


Ile Gln Met Gln Glu Leu Ile Lys Asn Cys Lys Glu Phe Gly Val Glu 
                85                  90                  95      


Leu Tyr Asp Leu Asn His Pro Tyr Gln Gly Ile Val His Val Met Gly 
            100                 105                 110         


Pro Glu Gln Gly Val Thr Leu Pro Gly Met Thr Ile Val Cys Gly Asp 
        115                 120                 125             


Ser His Thr Ala Thr His Gly Ala Phe Gly Ala Leu Ala Phe Gly Ile 
    130                 135                 140                 


Gly Thr Ser Glu Val Glu His Val Leu Ala Thr Gln Thr Leu Lys Gln 
145                 150                 155                 160 


Gly Arg Ala Lys Thr Met Lys Ile Glu Val Gln Gly Lys Ala Ala Pro 
                165                 170                 175     


Gly Ile Thr Ala Lys Asp Ile Val Leu Ala Ile Ile Gly Lys Thr Gly 
            180                 185                 190         


Ser Ala Gly Gly Thr Gly His Val Val Glu Phe Cys Gly Glu Ala Ile 
        195                 200                 205             


Arg Asp Leu Ser Met Glu Gly Arg Met Thr Leu Cys Asn Met Ala Ile 
    210                 215                 220                 


Glu Met Gly Ala Lys Ala Gly Leu Val Ala Pro Asp Glu Thr Thr Phe 
225                 230                 235                 240 


Asn Tyr Val Lys Gly Arg Leu His Ala Pro Lys Gly Lys Asp Phe Asp 
                245                 250                 255     


Asp Ala Val Ala Tyr Trp Lys Thr Leu Gln Thr Asp Glu Gly Ala Thr 
            260                 265                 270         


Phe Asp Thr Val Val Thr Leu Gln Ala Glu Glu Ile Ser Pro Gln Val 
        275                 280                 285             


Thr Trp Gly Thr Asn Pro Gly Gln Val Ile Ser Val Asn Asp Asn Ile 
    290                 295                 300                 


Pro Asp Pro Ala Ser Phe Ala Asp Pro Val Glu Arg Ala Ser Ala Glu 
305                 310                 315                 320 


Lys Ala Leu Ala Tyr Met Gly Leu Lys Pro Gly Ile Pro Leu Thr Glu 
                325                 330                 335     


Val Ala Ile Asp Lys Val Phe Ile Gly Ser Cys Thr Asn Ser Arg Ile 
            340                 345                 350         


Glu Asp Leu Arg Ala Ala Ala Glu Ile Ala Lys Gly Arg Lys Val Ala 
        355                 360                 365             


Pro Gly Val Gln Ala Leu Val Val Pro Gly Ser Gly Pro Val Lys Ala 
    370                 375                 380                 


Gln Ala Glu Ala Glu Gly Leu Asp Lys Ile Phe Ile Glu Ala Gly Phe 
385                 390                 395                 400 


Glu Trp Arg Leu Pro Gly Cys Ser Met Cys Ala Ala Met Asn Asn Asp 
                405                 410                 415     


Arg Leu Asn Pro Gly Glu Arg Cys Ala Ser Thr Ser Asn Arg Asn Phe 
            420                 425                 430         


Glu Gly Arg Gln Gly Arg Gly Gly Arg Thr His Leu Val Ser Pro Ala 
        435                 440                 445             


Met Ala Ala Ala Ala Ala Val Thr Gly His Phe Ala Asp Ile Arg Asn 
    450                 455                 460                 


Ile Lys 
465     


<210>  9
<211>  466
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthesized - Modified LeuC (V35A L411G)

<400>  9

Met Ala Lys Thr Leu Tyr Glu Lys Leu Phe Asp Ala His Val Val Tyr 
1               5                   10                  15      


Glu Ala Glu Asn Glu Thr Pro Leu Leu Tyr Ile Asp Arg His Leu Val 
            20                  25                  30          


His Glu Ala Thr Ser Pro Gln Ala Phe Asp Gly Leu Arg Ala His Gly 
        35                  40                  45              


Arg Pro Val Arg Gln Pro Gly Lys Thr Phe Ala Thr Met Asp His Asn 
    50                  55                  60                  


Val Ser Thr Gln Thr Lys Asp Ile Asn Ala Cys Gly Glu Met Ala Arg 
65                  70                  75                  80  


Ile Gln Met Gln Glu Leu Ile Lys Asn Cys Lys Glu Phe Gly Val Glu 
                85                  90                  95      


Leu Tyr Asp Leu Asn His Pro Tyr Gln Gly Ile Val His Val Met Gly 
            100                 105                 110         


Pro Glu Gln Gly Val Thr Leu Pro Gly Met Thr Ile Val Cys Gly Asp 
        115                 120                 125             


Ser His Thr Ala Thr His Gly Ala Phe Gly Ala Leu Ala Phe Gly Ile 
    130                 135                 140                 


Gly Thr Ser Glu Val Glu His Val Leu Ala Thr Gln Thr Leu Lys Gln 
145                 150                 155                 160 


Gly Arg Ala Lys Thr Met Lys Ile Glu Val Gln Gly Lys Ala Ala Pro 
                165                 170                 175     


Gly Ile Thr Ala Lys Asp Ile Val Leu Ala Ile Ile Gly Lys Thr Gly 
            180                 185                 190         


Ser Ala Gly Gly Thr Gly His Val Val Glu Phe Cys Gly Glu Ala Ile 
        195                 200                 205             


Arg Asp Leu Ser Met Glu Gly Arg Met Thr Leu Cys Asn Met Ala Ile 
    210                 215                 220                 


Glu Met Gly Ala Lys Ala Gly Leu Val Ala Pro Asp Glu Thr Thr Phe 
225                 230                 235                 240 


Asn Tyr Val Lys Gly Arg Leu His Ala Pro Lys Gly Lys Asp Phe Asp 
                245                 250                 255     


Asp Ala Val Ala Tyr Trp Lys Thr Leu Gln Thr Asp Glu Gly Ala Thr 
            260                 265                 270         


Phe Asp Thr Val Val Thr Leu Gln Ala Glu Glu Ile Ser Pro Gln Val 
        275                 280                 285             


Thr Trp Gly Thr Asn Pro Gly Gln Val Ile Ser Val Asn Asp Asn Ile 
    290                 295                 300                 


Pro Asp Pro Ala Ser Phe Ala Asp Pro Val Glu Arg Ala Ser Ala Glu 
305                 310                 315                 320 


Lys Ala Leu Ala Tyr Met Gly Leu Lys Pro Gly Ile Pro Leu Thr Glu 
                325                 330                 335     


Val Ala Ile Asp Lys Val Phe Ile Gly Ser Cys Thr Asn Ser Arg Ile 
            340                 345                 350         


Glu Asp Leu Arg Ala Ala Ala Glu Ile Ala Lys Gly Arg Lys Val Ala 
        355                 360                 365             


Pro Gly Val Gln Ala Leu Val Val Pro Gly Ser Gly Pro Val Lys Ala 
    370                 375                 380                 


Gln Ala Glu Ala Glu Gly Leu Asp Lys Ile Phe Ile Glu Ala Gly Phe 
385                 390                 395                 400 


Glu Trp Arg Leu Pro Gly Cys Ser Met Cys Gly Ala Met Asn Asn Asp 
                405                 410                 415     


Arg Leu Asn Pro Gly Glu Arg Cys Ala Ser Thr Ser Asn Arg Asn Phe 
            420                 425                 430         


Glu Gly Arg Gln Gly Arg Gly Gly Arg Thr His Leu Val Ser Pro Ala 
        435                 440                 445             


Met Ala Ala Ala Ala Ala Val Thr Gly His Phe Ala Asp Ile Arg Asn 
    450                 455                 460                 


Ile Lys 
465     


<210>  10
<211>  466
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthesized - Modified LeuC (V35A L411V)

<400>  10

Met Ala Lys Thr Leu Tyr Glu Lys Leu Phe Asp Ala His Val Val Tyr 
1               5                   10                  15      


Glu Ala Glu Asn Glu Thr Pro Leu Leu Tyr Ile Asp Arg His Leu Val 
            20                  25                  30          


His Glu Ala Thr Ser Pro Gln Ala Phe Asp Gly Leu Arg Ala His Gly 
        35                  40                  45              


Arg Pro Val Arg Gln Pro Gly Lys Thr Phe Ala Thr Met Asp His Asn 
    50                  55                  60                  


Val Ser Thr Gln Thr Lys Asp Ile Asn Ala Cys Gly Glu Met Ala Arg 
65                  70                  75                  80  


Ile Gln Met Gln Glu Leu Ile Lys Asn Cys Lys Glu Phe Gly Val Glu 
                85                  90                  95      


Leu Tyr Asp Leu Asn His Pro Tyr Gln Gly Ile Val His Val Met Gly 
            100                 105                 110         


Pro Glu Gln Gly Val Thr Leu Pro Gly Met Thr Ile Val Cys Gly Asp 
        115                 120                 125             


Ser His Thr Ala Thr His Gly Ala Phe Gly Ala Leu Ala Phe Gly Ile 
    130                 135                 140                 


Gly Thr Ser Glu Val Glu His Val Leu Ala Thr Gln Thr Leu Lys Gln 
145                 150                 155                 160 


Gly Arg Ala Lys Thr Met Lys Ile Glu Val Gln Gly Lys Ala Ala Pro 
                165                 170                 175     


Gly Ile Thr Ala Lys Asp Ile Val Leu Ala Ile Ile Gly Lys Thr Gly 
            180                 185                 190         


Ser Ala Gly Gly Thr Gly His Val Val Glu Phe Cys Gly Glu Ala Ile 
        195                 200                 205             


Arg Asp Leu Ser Met Glu Gly Arg Met Thr Leu Cys Asn Met Ala Ile 
    210                 215                 220                 


Glu Met Gly Ala Lys Ala Gly Leu Val Ala Pro Asp Glu Thr Thr Phe 
225                 230                 235                 240 


Asn Tyr Val Lys Gly Arg Leu His Ala Pro Lys Gly Lys Asp Phe Asp 
                245                 250                 255     


Asp Ala Val Ala Tyr Trp Lys Thr Leu Gln Thr Asp Glu Gly Ala Thr 
            260                 265                 270         


Phe Asp Thr Val Val Thr Leu Gln Ala Glu Glu Ile Ser Pro Gln Val 
        275                 280                 285             


Thr Trp Gly Thr Asn Pro Gly Gln Val Ile Ser Val Asn Asp Asn Ile 
    290                 295                 300                 


Pro Asp Pro Ala Ser Phe Ala Asp Pro Val Glu Arg Ala Ser Ala Glu 
305                 310                 315                 320 


Lys Ala Leu Ala Tyr Met Gly Leu Lys Pro Gly Ile Pro Leu Thr Glu 
                325                 330                 335     


Val Ala Ile Asp Lys Val Phe Ile Gly Ser Cys Thr Asn Ser Arg Ile 
            340                 345                 350         


Glu Asp Leu Arg Ala Ala Ala Glu Ile Ala Lys Gly Arg Lys Val Ala 
        355                 360                 365             


Pro Gly Val Gln Ala Leu Val Val Pro Gly Ser Gly Pro Val Lys Ala 
    370                 375                 380                 


Gln Ala Glu Ala Glu Gly Leu Asp Lys Ile Phe Ile Glu Ala Gly Phe 
385                 390                 395                 400 


Glu Trp Arg Leu Pro Gly Cys Ser Met Cys Val Ala Met Asn Asn Asp 
                405                 410                 415     


Arg Leu Asn Pro Gly Glu Arg Cys Ala Ser Thr Ser Asn Arg Asn Phe 
            420                 425                 430         


Glu Gly Arg Gln Gly Arg Gly Gly Arg Thr His Leu Val Ser Pro Ala 
        435                 440                 445             


Met Ala Ala Ala Ala Ala Val Thr Gly His Phe Ala Asp Ile Arg Asn 
    450                 455                 460                 


Ile Lys 
465     


<210>  11
<211>  466
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthesized - Modified LeuC (V35G L411A)

<400>  11

Met Ala Lys Thr Leu Tyr Glu Lys Leu Phe Asp Ala His Val Val Tyr 
1               5                   10                  15      


Glu Ala Glu Asn Glu Thr Pro Leu Leu Tyr Ile Asp Arg His Leu Val 
            20                  25                  30          


His Glu Gly Thr Ser Pro Gln Ala Phe Asp Gly Leu Arg Ala His Gly 
        35                  40                  45              


Arg Pro Val Arg Gln Pro Gly Lys Thr Phe Ala Thr Met Asp His Asn 
    50                  55                  60                  


Val Ser Thr Gln Thr Lys Asp Ile Asn Ala Cys Gly Glu Met Ala Arg 
65                  70                  75                  80  


Ile Gln Met Gln Glu Leu Ile Lys Asn Cys Lys Glu Phe Gly Val Glu 
                85                  90                  95      


Leu Tyr Asp Leu Asn His Pro Tyr Gln Gly Ile Val His Val Met Gly 
            100                 105                 110         


Pro Glu Gln Gly Val Thr Leu Pro Gly Met Thr Ile Val Cys Gly Asp 
        115                 120                 125             


Ser His Thr Ala Thr His Gly Ala Phe Gly Ala Leu Ala Phe Gly Ile 
    130                 135                 140                 


Gly Thr Ser Glu Val Glu His Val Leu Ala Thr Gln Thr Leu Lys Gln 
145                 150                 155                 160 


Gly Arg Ala Lys Thr Met Lys Ile Glu Val Gln Gly Lys Ala Ala Pro 
                165                 170                 175     


Gly Ile Thr Ala Lys Asp Ile Val Leu Ala Ile Ile Gly Lys Thr Gly 
            180                 185                 190         


Ser Ala Gly Gly Thr Gly His Val Val Glu Phe Cys Gly Glu Ala Ile 
        195                 200                 205             


Arg Asp Leu Ser Met Glu Gly Arg Met Thr Leu Cys Asn Met Ala Ile 
    210                 215                 220                 


Glu Met Gly Ala Lys Ala Gly Leu Val Ala Pro Asp Glu Thr Thr Phe 
225                 230                 235                 240 


Asn Tyr Val Lys Gly Arg Leu His Ala Pro Lys Gly Lys Asp Phe Asp 
                245                 250                 255     


Asp Ala Val Ala Tyr Trp Lys Thr Leu Gln Thr Asp Glu Gly Ala Thr 
            260                 265                 270         


Phe Asp Thr Val Val Thr Leu Gln Ala Glu Glu Ile Ser Pro Gln Val 
        275                 280                 285             


Thr Trp Gly Thr Asn Pro Gly Gln Val Ile Ser Val Asn Asp Asn Ile 
    290                 295                 300                 


Pro Asp Pro Ala Ser Phe Ala Asp Pro Val Glu Arg Ala Ser Ala Glu 
305                 310                 315                 320 


Lys Ala Leu Ala Tyr Met Gly Leu Lys Pro Gly Ile Pro Leu Thr Glu 
                325                 330                 335     


Val Ala Ile Asp Lys Val Phe Ile Gly Ser Cys Thr Asn Ser Arg Ile 
            340                 345                 350         


Glu Asp Leu Arg Ala Ala Ala Glu Ile Ala Lys Gly Arg Lys Val Ala 
        355                 360                 365             


Pro Gly Val Gln Ala Leu Val Val Pro Gly Ser Gly Pro Val Lys Ala 
    370                 375                 380                 


Gln Ala Glu Ala Glu Gly Leu Asp Lys Ile Phe Ile Glu Ala Gly Phe 
385                 390                 395                 400 


Glu Trp Arg Leu Pro Gly Cys Ser Met Cys Ala Ala Met Asn Asn Asp 
                405                 410                 415     


Arg Leu Asn Pro Gly Glu Arg Cys Ala Ser Thr Ser Asn Arg Asn Phe 
            420                 425                 430         


Glu Gly Arg Gln Gly Arg Gly Gly Arg Thr His Leu Val Ser Pro Ala 
        435                 440                 445             


Met Ala Ala Ala Ala Ala Val Thr Gly His Phe Ala Asp Ile Arg Asn 
    450                 455                 460                 


Ile Lys 
465     


<210>  12
<211>  466
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthesized - Modified LeuC (V35G L411G)

<400>  12

Met Ala Lys Thr Leu Tyr Glu Lys Leu Phe Asp Ala His Val Val Tyr 
1               5                   10                  15      


Glu Ala Glu Asn Glu Thr Pro Leu Leu Tyr Ile Asp Arg His Leu Val 
            20                  25                  30          


His Glu Gly Thr Ser Pro Gln Ala Phe Asp Gly Leu Arg Ala His Gly 
        35                  40                  45              


Arg Pro Val Arg Gln Pro Gly Lys Thr Phe Ala Thr Met Asp His Asn 
    50                  55                  60                  


Val Ser Thr Gln Thr Lys Asp Ile Asn Ala Cys Gly Glu Met Ala Arg 
65                  70                  75                  80  


Ile Gln Met Gln Glu Leu Ile Lys Asn Cys Lys Glu Phe Gly Val Glu 
                85                  90                  95      


Leu Tyr Asp Leu Asn His Pro Tyr Gln Gly Ile Val His Val Met Gly 
            100                 105                 110         


Pro Glu Gln Gly Val Thr Leu Pro Gly Met Thr Ile Val Cys Gly Asp 
        115                 120                 125             


Ser His Thr Ala Thr His Gly Ala Phe Gly Ala Leu Ala Phe Gly Ile 
    130                 135                 140                 


Gly Thr Ser Glu Val Glu His Val Leu Ala Thr Gln Thr Leu Lys Gln 
145                 150                 155                 160 


Gly Arg Ala Lys Thr Met Lys Ile Glu Val Gln Gly Lys Ala Ala Pro 
                165                 170                 175     


Gly Ile Thr Ala Lys Asp Ile Val Leu Ala Ile Ile Gly Lys Thr Gly 
            180                 185                 190         


Ser Ala Gly Gly Thr Gly His Val Val Glu Phe Cys Gly Glu Ala Ile 
        195                 200                 205             


Arg Asp Leu Ser Met Glu Gly Arg Met Thr Leu Cys Asn Met Ala Ile 
    210                 215                 220                 


Glu Met Gly Ala Lys Ala Gly Leu Val Ala Pro Asp Glu Thr Thr Phe 
225                 230                 235                 240 


Asn Tyr Val Lys Gly Arg Leu His Ala Pro Lys Gly Lys Asp Phe Asp 
                245                 250                 255     


Asp Ala Val Ala Tyr Trp Lys Thr Leu Gln Thr Asp Glu Gly Ala Thr 
            260                 265                 270         


Phe Asp Thr Val Val Thr Leu Gln Ala Glu Glu Ile Ser Pro Gln Val 
        275                 280                 285             


Thr Trp Gly Thr Asn Pro Gly Gln Val Ile Ser Val Asn Asp Asn Ile 
    290                 295                 300                 


Pro Asp Pro Ala Ser Phe Ala Asp Pro Val Glu Arg Ala Ser Ala Glu 
305                 310                 315                 320 


Lys Ala Leu Ala Tyr Met Gly Leu Lys Pro Gly Ile Pro Leu Thr Glu 
                325                 330                 335     


Val Ala Ile Asp Lys Val Phe Ile Gly Ser Cys Thr Asn Ser Arg Ile 
            340                 345                 350         


Glu Asp Leu Arg Ala Ala Ala Glu Ile Ala Lys Gly Arg Lys Val Ala 
        355                 360                 365             


Pro Gly Val Gln Ala Leu Val Val Pro Gly Ser Gly Pro Val Lys Ala 
    370                 375                 380                 


Gln Ala Glu Ala Glu Gly Leu Asp Lys Ile Phe Ile Glu Ala Gly Phe 
385                 390                 395                 400 


Glu Trp Arg Leu Pro Gly Cys Ser Met Cys Gly Ala Met Asn Asn Asp 
                405                 410                 415     


Arg Leu Asn Pro Gly Glu Arg Cys Ala Ser Thr Ser Asn Arg Asn Phe 
            420                 425                 430         


Glu Gly Arg Gln Gly Arg Gly Gly Arg Thr His Leu Val Ser Pro Ala 
        435                 440                 445             


Met Ala Ala Ala Ala Ala Val Thr Gly His Phe Ala Asp Ile Arg Asn 
    450                 455                 460                 


Ile Lys 
465     


<210>  13
<211>  466
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthesized - Modified LeuC (V35G L411V)

<400>  13

Met Ala Lys Thr Leu Tyr Glu Lys Leu Phe Asp Ala His Val Val Tyr 
1               5                   10                  15      


Glu Ala Glu Asn Glu Thr Pro Leu Leu Tyr Ile Asp Arg His Leu Val 
            20                  25                  30          


His Glu Gly Thr Ser Pro Gln Ala Phe Asp Gly Leu Arg Ala His Gly 
        35                  40                  45              


Arg Pro Val Arg Gln Pro Gly Lys Thr Phe Ala Thr Met Asp His Asn 
    50                  55                  60                  


Val Ser Thr Gln Thr Lys Asp Ile Asn Ala Cys Gly Glu Met Ala Arg 
65                  70                  75                  80  


Ile Gln Met Gln Glu Leu Ile Lys Asn Cys Lys Glu Phe Gly Val Glu 
                85                  90                  95      


Leu Tyr Asp Leu Asn His Pro Tyr Gln Gly Ile Val His Val Met Gly 
            100                 105                 110         


Pro Glu Gln Gly Val Thr Leu Pro Gly Met Thr Ile Val Cys Gly Asp 
        115                 120                 125             


Ser His Thr Ala Thr His Gly Ala Phe Gly Ala Leu Ala Phe Gly Ile 
    130                 135                 140                 


Gly Thr Ser Glu Val Glu His Val Leu Ala Thr Gln Thr Leu Lys Gln 
145                 150                 155                 160 


Gly Arg Ala Lys Thr Met Lys Ile Glu Val Gln Gly Lys Ala Ala Pro 
                165                 170                 175     


Gly Ile Thr Ala Lys Asp Ile Val Leu Ala Ile Ile Gly Lys Thr Gly 
            180                 185                 190         


Ser Ala Gly Gly Thr Gly His Val Val Glu Phe Cys Gly Glu Ala Ile 
        195                 200                 205             


Arg Asp Leu Ser Met Glu Gly Arg Met Thr Leu Cys Asn Met Ala Ile 
    210                 215                 220                 


Glu Met Gly Ala Lys Ala Gly Leu Val Ala Pro Asp Glu Thr Thr Phe 
225                 230                 235                 240 


Asn Tyr Val Lys Gly Arg Leu His Ala Pro Lys Gly Lys Asp Phe Asp 
                245                 250                 255     


Asp Ala Val Ala Tyr Trp Lys Thr Leu Gln Thr Asp Glu Gly Ala Thr 
            260                 265                 270         


Phe Asp Thr Val Val Thr Leu Gln Ala Glu Glu Ile Ser Pro Gln Val 
        275                 280                 285             


Thr Trp Gly Thr Asn Pro Gly Gln Val Ile Ser Val Asn Asp Asn Ile 
    290                 295                 300                 


Pro Asp Pro Ala Ser Phe Ala Asp Pro Val Glu Arg Ala Ser Ala Glu 
305                 310                 315                 320 


Lys Ala Leu Ala Tyr Met Gly Leu Lys Pro Gly Ile Pro Leu Thr Glu 
                325                 330                 335     


Val Ala Ile Asp Lys Val Phe Ile Gly Ser Cys Thr Asn Ser Arg Ile 
            340                 345                 350         


Glu Asp Leu Arg Ala Ala Ala Glu Ile Ala Lys Gly Arg Lys Val Ala 
        355                 360                 365             


Pro Gly Val Gln Ala Leu Val Val Pro Gly Ser Gly Pro Val Lys Ala 
    370                 375                 380                 


Gln Ala Glu Ala Glu Gly Leu Asp Lys Ile Phe Ile Glu Ala Gly Phe 
385                 390                 395                 400 


Glu Trp Arg Leu Pro Gly Cys Ser Met Cys Val Ala Met Asn Asn Asp 
                405                 410                 415     


Arg Leu Asn Pro Gly Glu Arg Cys Ala Ser Thr Ser Asn Arg Asn Phe 
            420                 425                 430         


Glu Gly Arg Gln Gly Arg Gly Gly Arg Thr His Leu Val Ser Pro Ala 
        435                 440                 445             


Met Ala Ala Ala Ala Ala Val Thr Gly His Phe Ala Asp Ile Arg Asn 
    450                 455                 460                 


Ile Lys 
465     


<210>  14
<211>  201
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthesized - Modified LeuD (H88A)

<400>  14

Met Ala Glu Lys Phe Ile Lys His Thr Gly Leu Val Val Pro Leu Asp 
1               5                   10                  15      


Ala Ala Asn Val Asp Thr Asp Ala Ile Ile Pro Lys Gln Phe Leu Gln 
            20                  25                  30          


Lys Val Thr Arg Thr Gly Phe Gly Ala His Leu Phe Asn Asp Trp Arg 
        35                  40                  45              


Phe Leu Asp Glu Lys Gly Gln Gln Pro Asn Pro Asp Phe Val Leu Asn 
    50                  55                  60                  


Phe Pro Gln Tyr Gln Gly Ala Ser Ile Leu Leu Ala Arg Glu Asn Phe 
65                  70                  75                  80  


Gly Cys Gly Ser Ser Arg Glu Ala Ala Pro Trp Ala Leu Thr Asp Tyr 
                85                  90                  95      


Gly Phe Lys Val Val Ile Ala Pro Ser Phe Ala Asp Ile Phe Tyr Gly 
            100                 105                 110         


Asn Ser Phe Asn Asn Gln Leu Leu Pro Val Lys Leu Ser Asp Ala Glu 
        115                 120                 125             


Val Asp Glu Leu Phe Ala Leu Val Lys Ala Asn Pro Gly Ile His Phe 
    130                 135                 140                 


Asp Val Asp Leu Glu Ala Gln Glu Val Lys Ala Gly Glu Lys Thr Tyr 
145                 150                 155                 160 


Arg Phe Thr Ile Asp Ala Phe Arg Arg His Cys Met Met Asn Gly Leu 
                165                 170                 175     


Asp Ser Ile Gly Leu Thr Leu Gln His Asp Asp Ala Ile Ala Ala Tyr 
            180                 185                 190         


Glu Ala Lys Gln Pro Ala Phe Met Asn 
        195                 200     


<210>  15
<211>  201
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthesized - Modified LeuD (H88G)

<400>  15

Met Ala Glu Lys Phe Ile Lys His Thr Gly Leu Val Val Pro Leu Asp 
1               5                   10                  15      


Ala Ala Asn Val Asp Thr Asp Ala Ile Ile Pro Lys Gln Phe Leu Gln 
            20                  25                  30          


Lys Val Thr Arg Thr Gly Phe Gly Ala His Leu Phe Asn Asp Trp Arg 
        35                  40                  45              


Phe Leu Asp Glu Lys Gly Gln Gln Pro Asn Pro Asp Phe Val Leu Asn 
    50                  55                  60                  


Phe Pro Gln Tyr Gln Gly Ala Ser Ile Leu Leu Ala Arg Glu Asn Phe 
65                  70                  75                  80  


Gly Cys Gly Ser Ser Arg Glu Gly Ala Pro Trp Ala Leu Thr Asp Tyr 
                85                  90                  95      


Gly Phe Lys Val Val Ile Ala Pro Ser Phe Ala Asp Ile Phe Tyr Gly 
            100                 105                 110         


Asn Ser Phe Asn Asn Gln Leu Leu Pro Val Lys Leu Ser Asp Ala Glu 
        115                 120                 125             


Val Asp Glu Leu Phe Ala Leu Val Lys Ala Asn Pro Gly Ile His Phe 
    130                 135                 140                 


Asp Val Asp Leu Glu Ala Gln Glu Val Lys Ala Gly Glu Lys Thr Tyr 
145                 150                 155                 160 


Arg Phe Thr Ile Asp Ala Phe Arg Arg His Cys Met Met Asn Gly Leu 
                165                 170                 175     


Asp Ser Ile Gly Leu Thr Leu Gln His Asp Asp Ala Ile Ala Ala Tyr 
            180                 185                 190         


Glu Ala Lys Gln Pro Ala Phe Met Asn 
        195                 200     


<210>  16
<211>  201
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthesized - Modified LeuD (H88V)

<400>  16

Met Ala Glu Lys Phe Ile Lys His Thr Gly Leu Val Val Pro Leu Asp 
1               5                   10                  15      


Ala Ala Asn Val Asp Thr Asp Ala Ile Ile Pro Lys Gln Phe Leu Gln 
            20                  25                  30          


Lys Val Thr Arg Thr Gly Phe Gly Ala His Leu Phe Asn Asp Trp Arg 
        35                  40                  45              


Phe Leu Asp Glu Lys Gly Gln Gln Pro Asn Pro Asp Phe Val Leu Asn 
    50                  55                  60                  


Phe Pro Gln Tyr Gln Gly Ala Ser Ile Leu Leu Ala Arg Glu Asn Phe 
65                  70                  75                  80  


Gly Cys Gly Ser Ser Arg Glu Val Ala Pro Trp Ala Leu Thr Asp Tyr 
                85                  90                  95      


Gly Phe Lys Val Val Ile Ala Pro Ser Phe Ala Asp Ile Phe Tyr Gly 
            100                 105                 110         


Asn Ser Phe Asn Asn Gln Leu Leu Pro Val Lys Leu Ser Asp Ala Glu 
        115                 120                 125             


Val Asp Glu Leu Phe Ala Leu Val Lys Ala Asn Pro Gly Ile His Phe 
    130                 135                 140                 


Asp Val Asp Leu Glu Ala Gln Glu Val Lys Ala Gly Glu Lys Thr Tyr 
145                 150                 155                 160 


Arg Phe Thr Ile Asp Ala Phe Arg Arg His Cys Met Met Asn Gly Leu 
                165                 170                 175     


Asp Ser Ile Gly Leu Thr Leu Gln His Asp Asp Ala Ile Ala Ala Tyr 
            180                 185                 190         


Glu Ala Lys Gln Pro Ala Phe Met Asn 
        195                 200     


<210>  17
<211>  201
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthesized - Modified LeuD (H88S)

<400>  17

Met Ala Glu Lys Phe Ile Lys His Thr Gly Leu Val Val Pro Leu Asp 
1               5                   10                  15      


Ala Ala Asn Val Asp Thr Asp Ala Ile Ile Pro Lys Gln Phe Leu Gln 
            20                  25                  30          


Lys Val Thr Arg Thr Gly Phe Gly Ala His Leu Phe Asn Asp Trp Arg 
        35                  40                  45              


Phe Leu Asp Glu Lys Gly Gln Gln Pro Asn Pro Asp Phe Val Leu Asn 
    50                  55                  60                  


Phe Pro Gln Tyr Gln Gly Ala Ser Ile Leu Leu Ala Arg Glu Asn Phe 
65                  70                  75                  80  


Gly Cys Gly Ser Ser Arg Glu Ser Ala Pro Trp Ala Leu Thr Asp Tyr 
                85                  90                  95      


Gly Phe Lys Val Val Ile Ala Pro Ser Phe Ala Asp Ile Phe Tyr Gly 
            100                 105                 110         


Asn Ser Phe Asn Asn Gln Leu Leu Pro Val Lys Leu Ser Asp Ala Glu 
        115                 120                 125             


Val Asp Glu Leu Phe Ala Leu Val Lys Ala Asn Pro Gly Ile His Phe 
    130                 135                 140                 


Asp Val Asp Leu Glu Ala Gln Glu Val Lys Ala Gly Glu Lys Thr Tyr 
145                 150                 155                 160 


Arg Phe Thr Ile Asp Ala Phe Arg Arg His Cys Met Met Asn Gly Leu 
                165                 170                 175     


Asp Ser Ile Gly Leu Thr Leu Gln His Asp Asp Ala Ile Ala Ala Tyr 
            180                 185                 190         


Glu Ala Lys Gln Pro Ala Phe Met Asn 
        195                 200     


<210>  18
<211>  201
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthesized - Modified LeuD (L31A)

<400>  18

Met Ala Glu Lys Phe Ile Lys His Thr Gly Leu Val Val Pro Leu Asp 
1               5                   10                  15      


Ala Ala Asn Val Asp Thr Asp Ala Ile Ile Pro Lys Gln Phe Ala Gln 
            20                  25                  30          


Lys Val Thr Arg Thr Gly Phe Gly Ala His Leu Phe Asn Asp Trp Arg 
        35                  40                  45              


Phe Leu Asp Glu Lys Gly Gln Gln Pro Asn Pro Asp Phe Val Leu Asn 
    50                  55                  60                  


Phe Pro Gln Tyr Gln Gly Ala Ser Ile Leu Leu Ala Arg Glu Asn Phe 
65                  70                  75                  80  


Gly Cys Gly Ser Ser Arg Glu His Ala Pro Trp Ala Leu Thr Asp Tyr 
                85                  90                  95      


Gly Phe Lys Val Val Ile Ala Pro Ser Phe Ala Asp Ile Phe Tyr Gly 
            100                 105                 110         


Asn Ser Phe Asn Asn Gln Leu Leu Pro Val Lys Leu Ser Asp Ala Glu 
        115                 120                 125             


Val Asp Glu Leu Phe Ala Leu Val Lys Ala Asn Pro Gly Ile His Phe 
    130                 135                 140                 


Asp Val Asp Leu Glu Ala Gln Glu Val Lys Ala Gly Glu Lys Thr Tyr 
145                 150                 155                 160 


Arg Phe Thr Ile Asp Ala Phe Arg Arg His Cys Met Met Asn Gly Leu 
                165                 170                 175     


Asp Ser Ile Gly Leu Thr Leu Gln His Asp Asp Ala Ile Ala Ala Tyr 
            180                 185                 190         


Glu Ala Lys Gln Pro Ala Phe Met Asn 
        195                 200     


<210>  19
<211>  201
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthesized - Modified LeuD (L31A H88A)

<400>  19

Met Ala Glu Lys Phe Ile Lys His Thr Gly Leu Val Val Pro Leu Asp 
1               5                   10                  15      


Ala Ala Asn Val Asp Thr Asp Ala Ile Ile Pro Lys Gln Phe Ala Gln 
            20                  25                  30          


Lys Val Thr Arg Thr Gly Phe Gly Ala His Leu Phe Asn Asp Trp Arg 
        35                  40                  45              


Phe Leu Asp Glu Lys Gly Gln Gln Pro Asn Pro Asp Phe Val Leu Asn 
    50                  55                  60                  


Phe Pro Gln Tyr Gln Gly Ala Ser Ile Leu Leu Ala Arg Glu Asn Phe 
65                  70                  75                  80  


Gly Cys Gly Ser Ser Arg Glu Ala Ala Pro Trp Ala Leu Thr Asp Tyr 
                85                  90                  95      


Gly Phe Lys Val Val Ile Ala Pro Ser Phe Ala Asp Ile Phe Tyr Gly 
            100                 105                 110         


Asn Ser Phe Asn Asn Gln Leu Leu Pro Val Lys Leu Ser Asp Ala Glu 
        115                 120                 125             


Val Asp Glu Leu Phe Ala Leu Val Lys Ala Asn Pro Gly Ile His Phe 
    130                 135                 140                 


Asp Val Asp Leu Glu Ala Gln Glu Val Lys Ala Gly Glu Lys Thr Tyr 
145                 150                 155                 160 


Arg Phe Thr Ile Asp Ala Phe Arg Arg His Cys Met Met Asn Gly Leu 
                165                 170                 175     


Asp Ser Ile Gly Leu Thr Leu Gln His Asp Asp Ala Ile Ala Ala Tyr 
            180                 185                 190         


Glu Ala Lys Gln Pro Ala Phe Met Asn 
        195                 200     


<210>  20
<211>  201
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthesized - Modified LeuD (L31A H88G)

<400>  20

Met Ala Glu Lys Phe Ile Lys His Thr Gly Leu Val Val Pro Leu Asp 
1               5                   10                  15      


Ala Ala Asn Val Asp Thr Asp Ala Ile Ile Pro Lys Gln Phe Ala Gln 
            20                  25                  30          


Lys Val Thr Arg Thr Gly Phe Gly Ala His Leu Phe Asn Asp Trp Arg 
        35                  40                  45              


Phe Leu Asp Glu Lys Gly Gln Gln Pro Asn Pro Asp Phe Val Leu Asn 
    50                  55                  60                  


Phe Pro Gln Tyr Gln Gly Ala Ser Ile Leu Leu Ala Arg Glu Asn Phe 
65                  70                  75                  80  


Gly Cys Gly Ser Ser Arg Glu Gly Ala Pro Trp Ala Leu Thr Asp Tyr 
                85                  90                  95      


Gly Phe Lys Val Val Ile Ala Pro Ser Phe Ala Asp Ile Phe Tyr Gly 
            100                 105                 110         


Asn Ser Phe Asn Asn Gln Leu Leu Pro Val Lys Leu Ser Asp Ala Glu 
        115                 120                 125             


Val Asp Glu Leu Phe Ala Leu Val Lys Ala Asn Pro Gly Ile His Phe 
    130                 135                 140                 


Asp Val Asp Leu Glu Ala Gln Glu Val Lys Ala Gly Glu Lys Thr Tyr 
145                 150                 155                 160 


Arg Phe Thr Ile Asp Ala Phe Arg Arg His Cys Met Met Asn Gly Leu 
                165                 170                 175     


Asp Ser Ile Gly Leu Thr Leu Gln His Asp Asp Ala Ile Ala Ala Tyr 
            180                 185                 190         


Glu Ala Lys Gln Pro Ala Phe Met Asn 
        195                 200     


<210>  21
<211>  201
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthesized - Modified LeuD (L31A H88V)

<400>  21

Met Ala Glu Lys Phe Ile Lys His Thr Gly Leu Val Val Pro Leu Asp 
1               5                   10                  15      


Ala Ala Asn Val Asp Thr Asp Ala Ile Ile Pro Lys Gln Phe Ala Gln 
            20                  25                  30          


Lys Val Thr Arg Thr Gly Phe Gly Ala His Leu Phe Asn Asp Trp Arg 
        35                  40                  45              


Phe Leu Asp Glu Lys Gly Gln Gln Pro Asn Pro Asp Phe Val Leu Asn 
    50                  55                  60                  


Phe Pro Gln Tyr Gln Gly Ala Ser Ile Leu Leu Ala Arg Glu Asn Phe 
65                  70                  75                  80  


Gly Cys Gly Ser Ser Arg Glu Val Ala Pro Trp Ala Leu Thr Asp Tyr 
                85                  90                  95      


Gly Phe Lys Val Val Ile Ala Pro Ser Phe Ala Asp Ile Phe Tyr Gly 
            100                 105                 110         


Asn Ser Phe Asn Asn Gln Leu Leu Pro Val Lys Leu Ser Asp Ala Glu 
        115                 120                 125             


Val Asp Glu Leu Phe Ala Leu Val Lys Ala Asn Pro Gly Ile His Phe 
    130                 135                 140                 


Asp Val Asp Leu Glu Ala Gln Glu Val Lys Ala Gly Glu Lys Thr Tyr 
145                 150                 155                 160 


Arg Phe Thr Ile Asp Ala Phe Arg Arg His Cys Met Met Asn Gly Leu 
                165                 170                 175     


Asp Ser Ile Gly Leu Thr Leu Gln His Asp Asp Ala Ile Ala Ala Tyr 
            180                 185                 190         


Glu Ala Lys Gln Pro Ala Phe Met Asn 
        195                 200     


<210>  22
<211>  201
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthesized - Modified LeuD (L31A H88S)

<400>  22

Met Ala Glu Lys Phe Ile Lys His Thr Gly Leu Val Val Pro Leu Asp 
1               5                   10                  15      


Ala Ala Asn Val Asp Thr Asp Ala Ile Ile Pro Lys Gln Phe Ala Gln 
            20                  25                  30          


Lys Val Thr Arg Thr Gly Phe Gly Ala His Leu Phe Asn Asp Trp Arg 
        35                  40                  45              


Phe Leu Asp Glu Lys Gly Gln Gln Pro Asn Pro Asp Phe Val Leu Asn 
    50                  55                  60                  


Phe Pro Gln Tyr Gln Gly Ala Ser Ile Leu Leu Ala Arg Glu Asn Phe 
65                  70                  75                  80  


Gly Cys Gly Ser Ser Arg Glu Ser Ala Pro Trp Ala Leu Thr Asp Tyr 
                85                  90                  95      


Gly Phe Lys Val Val Ile Ala Pro Ser Phe Ala Asp Ile Phe Tyr Gly 
            100                 105                 110         


Asn Ser Phe Asn Asn Gln Leu Leu Pro Val Lys Leu Ser Asp Ala Glu 
        115                 120                 125             


Val Asp Glu Leu Phe Ala Leu Val Lys Ala Asn Pro Gly Ile His Phe 
    130                 135                 140                 


Asp Val Asp Leu Glu Ala Gln Glu Val Lys Ala Gly Glu Lys Thr Tyr 
145                 150                 155                 160 


Arg Phe Thr Ile Asp Ala Phe Arg Arg His Cys Met Met Asn Gly Leu 
                165                 170                 175     


Asp Ser Ile Gly Leu Thr Leu Gln His Asp Asp Ala Ile Ala Ala Tyr 
            180                 185                 190         


Glu Ala Lys Gln Pro Ala Phe Met Asn 
        195                 200     


<210>  23
<211>  201
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthesized - Modified LeuD (L31G)

<400>  23

Met Ala Glu Lys Phe Ile Lys His Thr Gly Leu Val Val Pro Leu Asp 
1               5                   10                  15      


Ala Ala Asn Val Asp Thr Asp Ala Ile Ile Pro Lys Gln Phe Gly Gln 
            20                  25                  30          


Lys Val Thr Arg Thr Gly Phe Gly Ala His Leu Phe Asn Asp Trp Arg 
        35                  40                  45              


Phe Leu Asp Glu Lys Gly Gln Gln Pro Asn Pro Asp Phe Val Leu Asn 
    50                  55                  60                  


Phe Pro Gln Tyr Gln Gly Ala Ser Ile Leu Leu Ala Arg Glu Asn Phe 
65                  70                  75                  80  


Gly Cys Gly Ser Ser Arg Glu His Ala Pro Trp Ala Leu Thr Asp Tyr 
                85                  90                  95      


Gly Phe Lys Val Val Ile Ala Pro Ser Phe Ala Asp Ile Phe Tyr Gly 
            100                 105                 110         


Asn Ser Phe Asn Asn Gln Leu Leu Pro Val Lys Leu Ser Asp Ala Glu 
        115                 120                 125             


Val Asp Glu Leu Phe Ala Leu Val Lys Ala Asn Pro Gly Ile His Phe 
    130                 135                 140                 


Asp Val Asp Leu Glu Ala Gln Glu Val Lys Ala Gly Glu Lys Thr Tyr 
145                 150                 155                 160 


Arg Phe Thr Ile Asp Ala Phe Arg Arg His Cys Met Met Asn Gly Leu 
                165                 170                 175     


Asp Ser Ile Gly Leu Thr Leu Gln His Asp Asp Ala Ile Ala Ala Tyr 
            180                 185                 190         


Glu Ala Lys Gln Pro Ala Phe Met Asn 
        195                 200     


<210>  24
<211>  201
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthesized - Modified LeuD (L31G H88A)

<400>  24

Met Ala Glu Lys Phe Ile Lys His Thr Gly Leu Val Val Pro Leu Asp 
1               5                   10                  15      


Ala Ala Asn Val Asp Thr Asp Ala Ile Ile Pro Lys Gln Phe Gly Gln 
            20                  25                  30          


Lys Val Thr Arg Thr Gly Phe Gly Ala His Leu Phe Asn Asp Trp Arg 
        35                  40                  45              


Phe Leu Asp Glu Lys Gly Gln Gln Pro Asn Pro Asp Phe Val Leu Asn 
    50                  55                  60                  


Phe Pro Gln Tyr Gln Gly Ala Ser Ile Leu Leu Ala Arg Glu Asn Phe 
65                  70                  75                  80  


Gly Cys Gly Ser Ser Arg Glu Ala Ala Pro Trp Ala Leu Thr Asp Tyr 
                85                  90                  95      


Gly Phe Lys Val Val Ile Ala Pro Ser Phe Ala Asp Ile Phe Tyr Gly 
            100                 105                 110         


Asn Ser Phe Asn Asn Gln Leu Leu Pro Val Lys Leu Ser Asp Ala Glu 
        115                 120                 125             


Val Asp Glu Leu Phe Ala Leu Val Lys Ala Asn Pro Gly Ile His Phe 
    130                 135                 140                 


Asp Val Asp Leu Glu Ala Gln Glu Val Lys Ala Gly Glu Lys Thr Tyr 
145                 150                 155                 160 


Arg Phe Thr Ile Asp Ala Phe Arg Arg His Cys Met Met Asn Gly Leu 
                165                 170                 175     


Asp Ser Ile Gly Leu Thr Leu Gln His Asp Asp Ala Ile Ala Ala Tyr 
            180                 185                 190         


Glu Ala Lys Gln Pro Ala Phe Met Asn 
        195                 200     


<210>  25
<211>  201
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthesized - Modified LeuD (L31G H88G)

<400>  25

Met Ala Glu Lys Phe Ile Lys His Thr Gly Leu Val Val Pro Leu Asp 
1               5                   10                  15      


Ala Ala Asn Val Asp Thr Asp Ala Ile Ile Pro Lys Gln Phe Gly Gln 
            20                  25                  30          


Lys Val Thr Arg Thr Gly Phe Gly Ala His Leu Phe Asn Asp Trp Arg 
        35                  40                  45              


Phe Leu Asp Glu Lys Gly Gln Gln Pro Asn Pro Asp Phe Val Leu Asn 
    50                  55                  60                  


Phe Pro Gln Tyr Gln Gly Ala Ser Ile Leu Leu Ala Arg Glu Asn Phe 
65                  70                  75                  80  


Gly Cys Gly Ser Ser Arg Glu Gly Ala Pro Trp Ala Leu Thr Asp Tyr 
                85                  90                  95      


Gly Phe Lys Val Val Ile Ala Pro Ser Phe Ala Asp Ile Phe Tyr Gly 
            100                 105                 110         


Asn Ser Phe Asn Asn Gln Leu Leu Pro Val Lys Leu Ser Asp Ala Glu 
        115                 120                 125             


Val Asp Glu Leu Phe Ala Leu Val Lys Ala Asn Pro Gly Ile His Phe 
    130                 135                 140                 


Asp Val Asp Leu Glu Ala Gln Glu Val Lys Ala Gly Glu Lys Thr Tyr 
145                 150                 155                 160 


Arg Phe Thr Ile Asp Ala Phe Arg Arg His Cys Met Met Asn Gly Leu 
                165                 170                 175     


Asp Ser Ile Gly Leu Thr Leu Gln His Asp Asp Ala Ile Ala Ala Tyr 
            180                 185                 190         


Glu Ala Lys Gln Pro Ala Phe Met Asn 
        195                 200     


<210>  26
<211>  201
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthesized - Modified LeuD (L31G H88V)

<400>  26

Met Ala Glu Lys Phe Ile Lys His Thr Gly Leu Val Val Pro Leu Asp 
1               5                   10                  15      


Ala Ala Asn Val Asp Thr Asp Ala Ile Ile Pro Lys Gln Phe Gly Gln 
            20                  25                  30          


Lys Val Thr Arg Thr Gly Phe Gly Ala His Leu Phe Asn Asp Trp Arg 
        35                  40                  45              


Phe Leu Asp Glu Lys Gly Gln Gln Pro Asn Pro Asp Phe Val Leu Asn 
    50                  55                  60                  


Phe Pro Gln Tyr Gln Gly Ala Ser Ile Leu Leu Ala Arg Glu Asn Phe 
65                  70                  75                  80  


Gly Cys Gly Ser Ser Arg Glu Val Ala Pro Trp Ala Leu Thr Asp Tyr 
                85                  90                  95      


Gly Phe Lys Val Val Ile Ala Pro Ser Phe Ala Asp Ile Phe Tyr Gly 
            100                 105                 110         


Asn Ser Phe Asn Asn Gln Leu Leu Pro Val Lys Leu Ser Asp Ala Glu 
        115                 120                 125             


Val Asp Glu Leu Phe Ala Leu Val Lys Ala Asn Pro Gly Ile His Phe 
    130                 135                 140                 


Asp Val Asp Leu Glu Ala Gln Glu Val Lys Ala Gly Glu Lys Thr Tyr 
145                 150                 155                 160 


Arg Phe Thr Ile Asp Ala Phe Arg Arg His Cys Met Met Asn Gly Leu 
                165                 170                 175     


Asp Ser Ile Gly Leu Thr Leu Gln His Asp Asp Ala Ile Ala Ala Tyr 
            180                 185                 190         


Glu Ala Lys Gln Pro Ala Phe Met Asn 
        195                 200     


<210>  27
<211>  201
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthesized - Modified LeuD( L31G H88S)

<400>  27

Met Ala Glu Lys Phe Ile Lys His Thr Gly Leu Val Val Pro Leu Asp 
1               5                   10                  15      


Ala Ala Asn Val Asp Thr Asp Ala Ile Ile Pro Lys Gln Phe Gly Gln 
            20                  25                  30          


Lys Val Thr Arg Thr Gly Phe Gly Ala His Leu Phe Asn Asp Trp Arg 
        35                  40                  45              


Phe Leu Asp Glu Lys Gly Gln Gln Pro Asn Pro Asp Phe Val Leu Asn 
    50                  55                  60                  


Phe Pro Gln Tyr Gln Gly Ala Ser Ile Leu Leu Ala Arg Glu Asn Phe 
65                  70                  75                  80  


Gly Cys Gly Ser Ser Arg Glu Ser Ala Pro Trp Ala Leu Thr Asp Tyr 
                85                  90                  95      


Gly Phe Lys Val Val Ile Ala Pro Ser Phe Ala Asp Ile Phe Tyr Gly 
            100                 105                 110         


Asn Ser Phe Asn Asn Gln Leu Leu Pro Val Lys Leu Ser Asp Ala Glu 
        115                 120                 125             


Val Asp Glu Leu Phe Ala Leu Val Lys Ala Asn Pro Gly Ile His Phe 
    130                 135                 140                 


Asp Val Asp Leu Glu Ala Gln Glu Val Lys Ala Gly Glu Lys Thr Tyr 
145                 150                 155                 160 


Arg Phe Thr Ile Asp Ala Phe Arg Arg His Cys Met Met Asn Gly Leu 
                165                 170                 175     


Asp Ser Ile Gly Leu Thr Leu Gln His Asp Asp Ala Ile Ala Ala Tyr 
            180                 185                 190         


Glu Ala Lys Gln Pro Ala Phe Met Asn 
        195                 200     


<210>  28
<211>  201
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthesized - Modified LeuD (L31V)

<400>  28

Met Ala Glu Lys Phe Ile Lys His Thr Gly Leu Val Val Pro Leu Asp 
1               5                   10                  15      


Ala Ala Asn Val Asp Thr Asp Ala Ile Ile Pro Lys Gln Phe Val Gln 
            20                  25                  30          


Lys Val Thr Arg Thr Gly Phe Gly Ala His Leu Phe Asn Asp Trp Arg 
        35                  40                  45              


Phe Leu Asp Glu Lys Gly Gln Gln Pro Asn Pro Asp Phe Val Leu Asn 
    50                  55                  60                  


Phe Pro Gln Tyr Gln Gly Ala Ser Ile Leu Leu Ala Arg Glu Asn Phe 
65                  70                  75                  80  


Gly Cys Gly Ser Ser Arg Glu His Ala Pro Trp Ala Leu Thr Asp Tyr 
                85                  90                  95      


Gly Phe Lys Val Val Ile Ala Pro Ser Phe Ala Asp Ile Phe Tyr Gly 
            100                 105                 110         


Asn Ser Phe Asn Asn Gln Leu Leu Pro Val Lys Leu Ser Asp Ala Glu 
        115                 120                 125             


Val Asp Glu Leu Phe Ala Leu Val Lys Ala Asn Pro Gly Ile His Phe 
    130                 135                 140                 


Asp Val Asp Leu Glu Ala Gln Glu Val Lys Ala Gly Glu Lys Thr Tyr 
145                 150                 155                 160 


Arg Phe Thr Ile Asp Ala Phe Arg Arg His Cys Met Met Asn Gly Leu 
                165                 170                 175     


Asp Ser Ile Gly Leu Thr Leu Gln His Asp Asp Ala Ile Ala Ala Tyr 
            180                 185                 190         


Glu Ala Lys Gln Pro Ala Phe Met Asn 
        195                 200     


<210>  29
<211>  201
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthesized - Modified LeuD (L31V H88A)

<400>  29

Met Ala Glu Lys Phe Ile Lys His Thr Gly Leu Val Val Pro Leu Asp 
1               5                   10                  15      


Ala Ala Asn Val Asp Thr Asp Ala Ile Ile Pro Lys Gln Phe Val Gln 
            20                  25                  30          


Lys Val Thr Arg Thr Gly Phe Gly Ala His Leu Phe Asn Asp Trp Arg 
        35                  40                  45              


Phe Leu Asp Glu Lys Gly Gln Gln Pro Asn Pro Asp Phe Val Leu Asn 
    50                  55                  60                  


Phe Pro Gln Tyr Gln Gly Ala Ser Ile Leu Leu Ala Arg Glu Asn Phe 
65                  70                  75                  80  


Gly Cys Gly Ser Ser Arg Glu Ala Ala Pro Trp Ala Leu Thr Asp Tyr 
                85                  90                  95      


Gly Phe Lys Val Val Ile Ala Pro Ser Phe Ala Asp Ile Phe Tyr Gly 
            100                 105                 110         


Asn Ser Phe Asn Asn Gln Leu Leu Pro Val Lys Leu Ser Asp Ala Glu 
        115                 120                 125             


Val Asp Glu Leu Phe Ala Leu Val Lys Ala Asn Pro Gly Ile His Phe 
    130                 135                 140                 


Asp Val Asp Leu Glu Ala Gln Glu Val Lys Ala Gly Glu Lys Thr Tyr 
145                 150                 155                 160 


Arg Phe Thr Ile Asp Ala Phe Arg Arg His Cys Met Met Asn Gly Leu 
                165                 170                 175     


Asp Ser Ile Gly Leu Thr Leu Gln His Asp Asp Ala Ile Ala Ala Tyr 
            180                 185                 190         


Glu Ala Lys Gln Pro Ala Phe Met Asn 
        195                 200     


<210>  30
<211>  201
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthesized - Modified LeuD (L31V H88G)

<400>  30

Met Ala Glu Lys Phe Ile Lys His Thr Gly Leu Val Val Pro Leu Asp 
1               5                   10                  15      


Ala Ala Asn Val Asp Thr Asp Ala Ile Ile Pro Lys Gln Phe Val Gln 
            20                  25                  30          


Lys Val Thr Arg Thr Gly Phe Gly Ala His Leu Phe Asn Asp Trp Arg 
        35                  40                  45              


Phe Leu Asp Glu Lys Gly Gln Gln Pro Asn Pro Asp Phe Val Leu Asn 
    50                  55                  60                  


Phe Pro Gln Tyr Gln Gly Ala Ser Ile Leu Leu Ala Arg Glu Asn Phe 
65                  70                  75                  80  


Gly Cys Gly Ser Ser Arg Glu Gly Ala Pro Trp Ala Leu Thr Asp Tyr 
                85                  90                  95      


Gly Phe Lys Val Val Ile Ala Pro Ser Phe Ala Asp Ile Phe Tyr Gly 
            100                 105                 110         


Asn Ser Phe Asn Asn Gln Leu Leu Pro Val Lys Leu Ser Asp Ala Glu 
        115                 120                 125             


Val Asp Glu Leu Phe Ala Leu Val Lys Ala Asn Pro Gly Ile His Phe 
    130                 135                 140                 


Asp Val Asp Leu Glu Ala Gln Glu Val Lys Ala Gly Glu Lys Thr Tyr 
145                 150                 155                 160 


Arg Phe Thr Ile Asp Ala Phe Arg Arg His Cys Met Met Asn Gly Leu 
                165                 170                 175     


Asp Ser Ile Gly Leu Thr Leu Gln His Asp Asp Ala Ile Ala Ala Tyr 
            180                 185                 190         


Glu Ala Lys Gln Pro Ala Phe Met Asn 
        195                 200     


<210>  31
<211>  201
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthesized - Modified LeuD (L31V H88V)

<400>  31

Met Ala Glu Lys Phe Ile Lys His Thr Gly Leu Val Val Pro Leu Asp 
1               5                   10                  15      


Ala Ala Asn Val Asp Thr Asp Ala Ile Ile Pro Lys Gln Phe Val Gln 
            20                  25                  30          


Lys Val Thr Arg Thr Gly Phe Gly Ala His Leu Phe Asn Asp Trp Arg 
        35                  40                  45              


Phe Leu Asp Glu Lys Gly Gln Gln Pro Asn Pro Asp Phe Val Leu Asn 
    50                  55                  60                  


Phe Pro Gln Tyr Gln Gly Ala Ser Ile Leu Leu Ala Arg Glu Asn Phe 
65                  70                  75                  80  


Gly Cys Gly Ser Ser Arg Glu Val Ala Pro Trp Ala Leu Thr Asp Tyr 
                85                  90                  95      


Gly Phe Lys Val Val Ile Ala Pro Ser Phe Ala Asp Ile Phe Tyr Gly 
            100                 105                 110         


Asn Ser Phe Asn Asn Gln Leu Leu Pro Val Lys Leu Ser Asp Ala Glu 
        115                 120                 125             


Val Asp Glu Leu Phe Ala Leu Val Lys Ala Asn Pro Gly Ile His Phe 
    130                 135                 140                 


Asp Val Asp Leu Glu Ala Gln Glu Val Lys Ala Gly Glu Lys Thr Tyr 
145                 150                 155                 160 


Arg Phe Thr Ile Asp Ala Phe Arg Arg His Cys Met Met Asn Gly Leu 
                165                 170                 175     


Asp Ser Ile Gly Leu Thr Leu Gln His Asp Asp Ala Ile Ala Ala Tyr 
            180                 185                 190         


Glu Ala Lys Gln Pro Ala Phe Met Asn 
        195                 200     


<210>  32
<211>  201
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthesized - Modified LeuD (L31V H88S)

<400>  32

Met Ala Glu Lys Phe Ile Lys His Thr Gly Leu Val Val Pro Leu Asp 
1               5                   10                  15      


Ala Ala Asn Val Asp Thr Asp Ala Ile Ile Pro Lys Gln Phe Val Gln 
            20                  25                  30          


Lys Val Thr Arg Thr Gly Phe Gly Ala His Leu Phe Asn Asp Trp Arg 
        35                  40                  45              


Phe Leu Asp Glu Lys Gly Gln Gln Pro Asn Pro Asp Phe Val Leu Asn 
    50                  55                  60                  


Phe Pro Gln Tyr Gln Gly Ala Ser Ile Leu Leu Ala Arg Glu Asn Phe 
65                  70                  75                  80  


Gly Cys Gly Ser Ser Arg Glu Ser Ala Pro Trp Ala Leu Thr Asp Tyr 
                85                  90                  95      


Gly Phe Lys Val Val Ile Ala Pro Ser Phe Ala Asp Ile Phe Tyr Gly 
            100                 105                 110         


Asn Ser Phe Asn Asn Gln Leu Leu Pro Val Lys Leu Ser Asp Ala Glu 
        115                 120                 125             


Val Asp Glu Leu Phe Ala Leu Val Lys Ala Asn Pro Gly Ile His Phe 
    130                 135                 140                 


Asp Val Asp Leu Glu Ala Gln Glu Val Lys Ala Gly Glu Lys Thr Tyr 
145                 150                 155                 160 


Arg Phe Thr Ile Asp Ala Phe Arg Arg His Cys Met Met Asn Gly Leu 
                165                 170                 175     


Asp Ser Ile Gly Leu Thr Leu Gln His Asp Asp Ala Ile Ala Ala Tyr 
            180                 185                 190         


Glu Ala Lys Gln Pro Ala Phe Met Asn 
        195                 200     


<210>  33
<211>  201
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthesized - Modified LeuD (L31S)

<400>  33

Met Ala Glu Lys Phe Ile Lys His Thr Gly Leu Val Val Pro Leu Asp 
1               5                   10                  15      


Ala Ala Asn Val Asp Thr Asp Ala Ile Ile Pro Lys Gln Phe Ser Gln 
            20                  25                  30          


Lys Val Thr Arg Thr Gly Phe Gly Ala His Leu Phe Asn Asp Trp Arg 
        35                  40                  45              


Phe Leu Asp Glu Lys Gly Gln Gln Pro Asn Pro Asp Phe Val Leu Asn 
    50                  55                  60                  


Phe Pro Gln Tyr Gln Gly Ala Ser Ile Leu Leu Ala Arg Glu Asn Phe 
65                  70                  75                  80  


Gly Cys Gly Ser Ser Arg Glu His Ala Pro Trp Ala Leu Thr Asp Tyr 
                85                  90                  95      


Gly Phe Lys Val Val Ile Ala Pro Ser Phe Ala Asp Ile Phe Tyr Gly 
            100                 105                 110         


Asn Ser Phe Asn Asn Gln Leu Leu Pro Val Lys Leu Ser Asp Ala Glu 
        115                 120                 125             


Val Asp Glu Leu Phe Ala Leu Val Lys Ala Asn Pro Gly Ile His Phe 
    130                 135                 140                 


Asp Val Asp Leu Glu Ala Gln Glu Val Lys Ala Gly Glu Lys Thr Tyr 
145                 150                 155                 160 


Arg Phe Thr Ile Asp Ala Phe Arg Arg His Cys Met Met Asn Gly Leu 
                165                 170                 175     


Asp Ser Ile Gly Leu Thr Leu Gln His Asp Asp Ala Ile Ala Ala Tyr 
            180                 185                 190         


Glu Ala Lys Gln Pro Ala Phe Met Asn 
        195                 200     


<210>  34
<211>  201
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthesized - Modified LeuD (L31S H88A)

<400>  34

Met Ala Glu Lys Phe Ile Lys His Thr Gly Leu Val Val Pro Leu Asp 
1               5                   10                  15      


Ala Ala Asn Val Asp Thr Asp Ala Ile Ile Pro Lys Gln Phe Ser Gln 
            20                  25                  30          


Lys Val Thr Arg Thr Gly Phe Gly Ala His Leu Phe Asn Asp Trp Arg 
        35                  40                  45              


Phe Leu Asp Glu Lys Gly Gln Gln Pro Asn Pro Asp Phe Val Leu Asn 
    50                  55                  60                  


Phe Pro Gln Tyr Gln Gly Ala Ser Ile Leu Leu Ala Arg Glu Asn Phe 
65                  70                  75                  80  


Gly Cys Gly Ser Ser Arg Glu Ala Ala Pro Trp Ala Leu Thr Asp Tyr 
                85                  90                  95      


Gly Phe Lys Val Val Ile Ala Pro Ser Phe Ala Asp Ile Phe Tyr Gly 
            100                 105                 110         


Asn Ser Phe Asn Asn Gln Leu Leu Pro Val Lys Leu Ser Asp Ala Glu 
        115                 120                 125             


Val Asp Glu Leu Phe Ala Leu Val Lys Ala Asn Pro Gly Ile His Phe 
    130                 135                 140                 


Asp Val Asp Leu Glu Ala Gln Glu Val Lys Ala Gly Glu Lys Thr Tyr 
145                 150                 155                 160 


Arg Phe Thr Ile Asp Ala Phe Arg Arg His Cys Met Met Asn Gly Leu 
                165                 170                 175     


Asp Ser Ile Gly Leu Thr Leu Gln His Asp Asp Ala Ile Ala Ala Tyr 
            180                 185                 190         


Glu Ala Lys Gln Pro Ala Phe Met Asn 
        195                 200     


<210>  35
<211>  201
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthesized - Modified LeuD (L31S H88G)

<400>  35

Met Ala Glu Lys Phe Ile Lys His Thr Gly Leu Val Val Pro Leu Asp 
1               5                   10                  15      


Ala Ala Asn Val Asp Thr Asp Ala Ile Ile Pro Lys Gln Phe Ser Gln 
            20                  25                  30          


Lys Val Thr Arg Thr Gly Phe Gly Ala His Leu Phe Asn Asp Trp Arg 
        35                  40                  45              


Phe Leu Asp Glu Lys Gly Gln Gln Pro Asn Pro Asp Phe Val Leu Asn 
    50                  55                  60                  


Phe Pro Gln Tyr Gln Gly Ala Ser Ile Leu Leu Ala Arg Glu Asn Phe 
65                  70                  75                  80  


Gly Cys Gly Ser Ser Arg Glu Gly Ala Pro Trp Ala Leu Thr Asp Tyr 
                85                  90                  95      


Gly Phe Lys Val Val Ile Ala Pro Ser Phe Ala Asp Ile Phe Tyr Gly 
            100                 105                 110         


Asn Ser Phe Asn Asn Gln Leu Leu Pro Val Lys Leu Ser Asp Ala Glu 
        115                 120                 125             


Val Asp Glu Leu Phe Ala Leu Val Lys Ala Asn Pro Gly Ile His Phe 
    130                 135                 140                 


Asp Val Asp Leu Glu Ala Gln Glu Val Lys Ala Gly Glu Lys Thr Tyr 
145                 150                 155                 160 


Arg Phe Thr Ile Asp Ala Phe Arg Arg His Cys Met Met Asn Gly Leu 
                165                 170                 175     


Asp Ser Ile Gly Leu Thr Leu Gln His Asp Asp Ala Ile Ala Ala Tyr 
            180                 185                 190         


Glu Ala Lys Gln Pro Ala Phe Met Asn 
        195                 200     


<210>  36
<211>  201
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthesized - Modified LeuD (L31S H88V)

<400>  36

Met Ala Glu Lys Phe Ile Lys His Thr Gly Leu Val Val Pro Leu Asp 
1               5                   10                  15      


Ala Ala Asn Val Asp Thr Asp Ala Ile Ile Pro Lys Gln Phe Ser Gln 
            20                  25                  30          


Lys Val Thr Arg Thr Gly Phe Gly Ala His Leu Phe Asn Asp Trp Arg 
        35                  40                  45              


Phe Leu Asp Glu Lys Gly Gln Gln Pro Asn Pro Asp Phe Val Leu Asn 
    50                  55                  60                  


Phe Pro Gln Tyr Gln Gly Ala Ser Ile Leu Leu Ala Arg Glu Asn Phe 
65                  70                  75                  80  


Gly Cys Gly Ser Ser Arg Glu Val Ala Pro Trp Ala Leu Thr Asp Tyr 
                85                  90                  95      


Gly Phe Lys Val Val Ile Ala Pro Ser Phe Ala Asp Ile Phe Tyr Gly 
            100                 105                 110         


Asn Ser Phe Asn Asn Gln Leu Leu Pro Val Lys Leu Ser Asp Ala Glu 
        115                 120                 125             


Val Asp Glu Leu Phe Ala Leu Val Lys Ala Asn Pro Gly Ile His Phe 
    130                 135                 140                 


Asp Val Asp Leu Glu Ala Gln Glu Val Lys Ala Gly Glu Lys Thr Tyr 
145                 150                 155                 160 


Arg Phe Thr Ile Asp Ala Phe Arg Arg His Cys Met Met Asn Gly Leu 
                165                 170                 175     


Asp Ser Ile Gly Leu Thr Leu Gln His Asp Asp Ala Ile Ala Ala Tyr 
            180                 185                 190         


Glu Ala Lys Gln Pro Ala Phe Met Asn 
        195                 200     


<210>  37
<211>  201
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthesized - Modified LeuD (L31S H88S)

<400>  37

Met Ala Glu Lys Phe Ile Lys His Thr Gly Leu Val Val Pro Leu Asp 
1               5                   10                  15      


Ala Ala Asn Val Asp Thr Asp Ala Ile Ile Pro Lys Gln Phe Ser Gln 
            20                  25                  30          


Lys Val Thr Arg Thr Gly Phe Gly Ala His Leu Phe Asn Asp Trp Arg 
        35                  40                  45              


Phe Leu Asp Glu Lys Gly Gln Gln Pro Asn Pro Asp Phe Val Leu Asn 
    50                  55                  60                  


Phe Pro Gln Tyr Gln Gly Ala Ser Ile Leu Leu Ala Arg Glu Asn Phe 
65                  70                  75                  80  


Gly Cys Gly Ser Ser Arg Glu Ser Ala Pro Trp Ala Leu Thr Asp Tyr 
                85                  90                  95      


Gly Phe Lys Val Val Ile Ala Pro Ser Phe Ala Asp Ile Phe Tyr Gly 
            100                 105                 110         


Asn Ser Phe Asn Asn Gln Leu Leu Pro Val Lys Leu Ser Asp Ala Glu 
        115                 120                 125             


Val Asp Glu Leu Phe Ala Leu Val Lys Ala Asn Pro Gly Ile His Phe 
    130                 135                 140                 


Asp Val Asp Leu Glu Ala Gln Glu Val Lys Ala Gly Glu Lys Thr Tyr 
145                 150                 155                 160 


Arg Phe Thr Ile Asp Ala Phe Arg Arg His Cys Met Met Asn Gly Leu 
                165                 170                 175     


Asp Ser Ile Gly Leu Thr Leu Gln His Asp Asp Ala Ile Ala Ala Tyr 
            180                 185                 190         


Glu Ala Lys Gln Pro Ala Phe Met Asn 
        195                 200     


