                         SEQUENCE LISTING

<110>  BONNING , Bryony  C.
       KEMMERER , Mariah
 
<120>  Insect Toxin Delivery Mediated by a Densovirus Coat Protein

<130>  T2315-22736WO01

<150>  62/400,381
<151>  2016-09-27

<160>  16    

<170>  PatentIn version 3.5

<210>  1
<211>  810
<212>  PRT
<213>  Junonia coenia densovirus


<220>
<221>  misc_feature
<222>  (368)..(368)
<223>  Xaa can be any naturally occurring amino acid

<400>  1

Met Ser Phe Tyr Thr Ala Gly Leu Ile His Arg Ala Arg Pro Gly Tyr 
1               5                   10                  15      


Arg Ile Ile Pro Glu Ser Thr Ala Thr Glu Asp Ile Glu Leu Gly Ala 
            20                  25                  30          


Ile Gly Glu Glu Thr Pro Leu Leu Ser Glu Gly Ala Val Thr Ala Val 
        35                  40                  45              


Glu Glu Ser Ala Ala Val Gly Leu Pro Glu Leu Gly Ala Gly Leu Ala 
    50                  55                  60                  


Gly Ala Ile Gly Thr His Ala Asp Val Leu Tyr Arg Asn Arg Asn Val 
65                  70                  75                  80  


Phe Lys Ser Val Leu Thr Gly Asn Tyr Thr Asp Leu Lys Gly Asn Pro 
                85                  90                  95      


Leu Lys Gln Arg Asn Ala Ile Ser Glu Lys Thr Lys Gln Leu Gly Arg 
            100                 105                 110         


Gly Ile Phe Gln Gly Asp Phe Asn Arg Ala Phe Pro Asp Asp Leu Lys 
        115                 120                 125             


Leu Glu Thr Glu Gln Glu Lys Lys Asp Leu Leu Arg Tyr Tyr Asn His 
    130                 135                 140                 


Asn Arg Arg Leu Ala Gly Leu Ser Glu Ala Tyr Pro Gln Gly Lys Gly 
145                 150                 155                 160 


Tyr Ala Tyr Ala Lys Ser Gln Lys Val Leu Glu Ala Glu Arg Arg Gly 
                165                 170                 175     


Leu Thr Val Pro Gly Tyr Lys Tyr Leu Gly Pro Gly Asn Ser Leu Asn 
            180                 185                 190         


Arg Gly Gln Pro Thr Asn Gln Ile Asp Glu Asp Ala Lys Glu His Asp 
        195                 200                 205             


Glu Ala Tyr Asp Lys Ala Lys Thr Ser Gln Glu Val Ser Gln Ala Asp 
    210                 215                 220                 


Asn Thr Phe Val Asn Lys Ala Leu Asp His Ile Val Asn Ala Ile Asn 
225                 230                 235                 240 


Leu Lys Glu Thr Pro Gly Asn Ala Phe Gly Ala Ala Ile Gly Ala Ile 
                245                 250                 255     


Gly Ile Gly Thr Lys Gln Ala Ile Glu Lys His Ser Gly Val Ile Tyr 
            260                 265                 270         


Pro Ser Val Ser Gly Met Ser Arg Gln Ile Asn Ser Lys Tyr Leu Asn 
        275                 280                 285             


Ser Trp His Asp Trp Ile Glu Gln Asn Lys His Asn Asn Phe Glu Gly 
    290                 295                 300                 


Ile Gln Leu Pro Glu Asp Phe Tyr Thr Glu Glu Gln Thr Leu Ser Asp 
305                 310                 315                 320 


Ser Pro Met Ser Glu Gly Thr Lys Arg Lys Ala Asp Thr Pro Val Glu 
                325                 330                 335     


Glu Gly Pro Ser Lys Lys Gly Ala His Asn Ala Pro His Asn Ser Gln 
            340                 345                 350         


Gly Thr Asp Pro Gln Asn Pro Ser Ser Ser Gly Ala Thr Thr Ser Xaa 
        355                 360                 365             


Asp Val Glu Met Ala Met Ser Leu Pro Gly Thr Gly Ser Gly Thr Ser 
    370                 375                 380                 


Ser Gly Gly Gly Asn Thr Ser Gly Gln Glu Val Tyr Val Ile Pro Arg 
385                 390                 395                 400 


Pro Phe Ser Asn Phe Gly Lys Lys Leu Ser Thr Tyr Thr Lys Ser His 
                405                 410                 415     


Lys Phe Met Ile Phe Gly Leu Ala Asn Asn Val Ile Gly Pro Thr Gly 
            420                 425                 430         


Thr Gly Thr Thr Ala Val Asn Arg Leu Ile Thr Thr Cys Leu Ala Glu 
        435                 440                 445             


Ile Pro Trp Gln Lys Leu Pro Leu Tyr Met Asn Gln Ser Glu Phe Asp 
    450                 455                 460                 


Leu Leu Pro Pro Gly Ser Arg Val Val Glu Cys Asn Val Lys Val Ile 
465                 470                 475                 480 


Phe Arg Thr Asn Arg Ile Ala Phe Glu Thr Ser Ser Thr Ala Thr Lys 
                485                 490                 495     


Gln Ala Thr Leu Asn Gln Ile Ser Asn Leu Gln Thr Ala Val Gly Leu 
            500                 505                 510         


Asn Lys Leu Gly Trp Gly Ile Asp Arg Ser Phe Thr Ala Phe Gln Ser 
        515                 520                 525             


Asp Gln Pro Met Ile Pro Thr Ala Thr Ser Ala Pro Lys Tyr Glu Pro 
    530                 535                 540                 


Ile Thr Gly Thr Thr Gly Tyr Arg Gly Met Ile Ala Asp Tyr Tyr Gly 
545                 550                 555                 560 


Ala Asp Ser Thr Asn Asp Ala Ala Phe Gly Asn Ala Gly Asn Tyr Pro 
                565                 570                 575     


His His Gln Val Gly Ser Phe Thr Phe Ile Gln Asn Tyr Tyr Cys Met 
            580                 585                 590         


Tyr Gln Gln Thr Asn Gln Gly Thr Gly Gly Trp Pro Cys Leu Ala Glu 
        595                 600                 605             


His Leu Gln Gln Phe Asp Ser Lys Thr Val Asn Asn Gln Cys Leu Ile 
    610                 615                 620                 


Asp Val Thr Tyr Lys Pro Lys Met Gly Leu Ile Lys Pro Pro Leu Asn 
625                 630                 635                 640 


Tyr Lys Ile Ile Gly Gln Pro Thr Ala Lys Gly Thr Ile Ser Val Gly 
                645                 650                 655     


Asp Asn Leu Val Asn Met Arg Gly Ala Val Val Ile Asn Pro Pro Glu 
            660                 665                 670         


Ala Thr Gln Ser Val Thr Glu Ser Thr His Asn Leu Thr Arg Asn Phe 
        675                 680                 685             


Pro Ala Asn Leu Phe Asn Ile Tyr Ser Asp Ile Glu Lys Ser Gln Ile 
    690                 695                 700                 


Leu His Lys Gly Pro Trp Gly His Glu Asn Pro Gln Ile Gln Pro Ser 
705                 710                 715                 720 


Val His Ile Gly Ile Gln Ala Val Pro Ala Leu Thr Thr Gly Ala Leu 
                725                 730                 735     


Leu Val Asn Ser Ser Pro Leu Asn Ser Trp Thr Asp Ser Met Gly Tyr 
            740                 745                 750         


Ile Asp Val Met Ser Ser Cys Thr Val Met Glu Ser Gln Pro Thr His 
        755                 760                 765             


Phe Pro Phe Ser Thr Asp Ala Asn Thr Asn Pro Gly Asn Thr Ile Tyr 
    770                 775                 780                 


Arg Ile Asn Leu Thr Pro Asn Ser Leu Thr Ser Ala Phe Asn Gly Leu 
785                 790                 795                 800 


Tyr Gly Asn Gly Ala Thr Leu Gly Asn Val 
                805                 810 


<210>  2
<211>  437
<212>  PRT
<213>  Junonia coenia densovirus

<400>  2

Met Ser Leu Pro Gly Thr Gly Ser Gly Thr Ser Ser Gly Gly Gly Asn 
1               5                   10                  15      


Thr Ser Gly Gln Glu Val Tyr Val Ile Pro Arg Pro Phe Ser Asn Phe 
            20                  25                  30          


Gly Lys Lys Leu Ser Thr Tyr Thr Lys Ser His Lys Phe Met Ile Phe 
        35                  40                  45              


Gly Leu Ala Asn Asn Val Ile Gly Pro Thr Gly Thr Gly Thr Thr Ala 
    50                  55                  60                  


Val Asn Arg Leu Ile Thr Thr Cys Leu Ala Glu Ile Pro Trp Gln Lys 
65                  70                  75                  80  


Leu Pro Leu Tyr Met Asn Gln Ser Glu Phe Asp Leu Leu Pro Pro Gly 
                85                  90                  95      


Ser Arg Val Val Glu Cys Asn Val Lys Val Ile Phe Arg Thr Asn Arg 
            100                 105                 110         


Ile Ala Phe Glu Thr Ser Ser Thr Ala Thr Lys Gln Ala Thr Leu Asn 
        115                 120                 125             


Gln Ile Ser Asn Leu Gln Thr Ala Val Gly Leu Asn Lys Leu Gly Trp 
    130                 135                 140                 


Gly Ile Asp Arg Ser Phe Thr Ala Phe Gln Ser Asp Gln Pro Met Ile 
145                 150                 155                 160 


Pro Thr Ala Thr Ser Ala Pro Lys Tyr Glu Pro Ile Thr Gly Thr Thr 
                165                 170                 175     


Gly Tyr Arg Gly Met Ile Ala Asp Tyr Tyr Gly Ala Asp Ser Thr Asn 
            180                 185                 190         


Asp Ala Ala Phe Gly Asn Ala Gly Asn Tyr Pro His His Gln Val Gly 
        195                 200                 205             


Ser Phe Thr Phe Ile Gln Asn Tyr Tyr Cys Met Tyr Gln Gln Thr Asn 
    210                 215                 220                 


Gln Gly Thr Gly Gly Trp Pro Cys Leu Ala Glu His Leu Gln Gln Phe 
225                 230                 235                 240 


Asp Ser Lys Thr Val Asn Asn Gln Cys Leu Ile Asp Val Thr Tyr Lys 
                245                 250                 255     


Pro Lys Met Gly Leu Ile Lys Pro Pro Leu Asn Tyr Lys Ile Ile Gly 
            260                 265                 270         


Gln Pro Thr Ala Lys Gly Thr Ile Ser Val Gly Asp Asn Leu Val Asn 
        275                 280                 285             


Met Arg Gly Ala Val Val Ile Asn Pro Pro Glu Ala Thr Gln Ser Val 
    290                 295                 300                 


Thr Glu Ser Thr His Asn Leu Thr Arg Asn Phe Pro Ala Asn Leu Phe 
305                 310                 315                 320 


Asn Ile Tyr Ser Asp Ile Glu Lys Ser Gln Ile Leu His Lys Gly Pro 
                325                 330                 335     


Trp Gly His Glu Asn Pro Gln Ile Gln Pro Ser Val His Ile Gly Ile 
            340                 345                 350         


Gln Ala Val Pro Ala Leu Thr Thr Gly Ala Leu Leu Val Asn Ser Ser 
        355                 360                 365             


Pro Leu Asn Ser Trp Thr Asp Ser Met Gly Tyr Ile Asp Val Met Ser 
    370                 375                 380                 


Ser Cys Thr Val Met Glu Ser Gln Pro Thr His Phe Pro Phe Ser Thr 
385                 390                 395                 400 


Asp Ala Asn Thr Asn Pro Gly Asn Thr Ile Tyr Arg Ile Asn Leu Thr 
                405                 410                 415     


Pro Asn Ser Leu Thr Ser Ala Phe Asn Gly Leu Tyr Gly Asn Gly Ala 
            420                 425                 430         


Thr Leu Gly Asn Val 
        435         


<210>  3
<211>  704
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  fusion protein sequence of Junonia coenia Densovirus structural 
       protein VP4 fused to eGFP via a peptide linker

<400>  3

Met Ser Leu Pro Gly Thr Gly Ser Gly Thr Ser Ser Gly Gly Gly Asn 
1               5                   10                  15      


Thr Ser Gly Gln Glu Val Tyr Val Ile Pro Arg Pro Phe Ser Asn Phe 
            20                  25                  30          


Gly Lys Lys Leu Ser Thr Tyr Thr Lys Ser His Lys Phe Met Ile Phe 
        35                  40                  45              


Gly Leu Ala Asn Asn Val Ile Gly Pro Thr Gly Thr Gly Thr Thr Ala 
    50                  55                  60                  


Val Asn Arg Leu Ile Thr Thr Cys Leu Ala Glu Ile Pro Trp Gln Lys 
65                  70                  75                  80  


Leu Pro Leu Tyr Met Asn Gln Ser Glu Phe Asp Leu Leu Pro Pro Gly 
                85                  90                  95      


Ser Arg Val Val Glu Cys Asn Val Lys Val Ile Phe Arg Thr Asn Arg 
            100                 105                 110         


Ile Ala Phe Glu Thr Ser Ser Thr Ala Thr Lys Gln Ala Thr Leu Asn 
        115                 120                 125             


Gln Ile Ser Asn Leu Gln Thr Ala Val Gly Leu Asn Lys Leu Gly Trp 
    130                 135                 140                 


Gly Ile Asp Arg Ser Phe Thr Ala Phe Gln Ser Asp Gln Pro Met Ile 
145                 150                 155                 160 


Pro Thr Ala Thr Ser Ala Pro Lys Tyr Glu Pro Ile Thr Gly Thr Thr 
                165                 170                 175     


Gly Tyr Arg Gly Met Ile Ala Asp Tyr Tyr Gly Ala Asp Ser Thr Asn 
            180                 185                 190         


Asp Ala Ala Phe Gly Asn Ala Gly Asn Tyr Pro His His Gln Val Gly 
        195                 200                 205             


Ser Phe Thr Phe Ile Gln Asn Tyr Tyr Cys Met Tyr Gln Gln Thr Asn 
    210                 215                 220                 


Gln Gly Thr Gly Gly Trp Pro Cys Leu Ala Glu His Leu Gln Gln Phe 
225                 230                 235                 240 


Asp Ser Lys Thr Val Asn Asn Gln Cys Leu Ile Asp Val Thr Tyr Lys 
                245                 250                 255     


Pro Lys Met Gly Leu Ile Lys Pro Pro Leu Asn Tyr Lys Ile Ile Gly 
            260                 265                 270         


Gln Pro Thr Ala Lys Gly Thr Ile Ser Val Gly Asp Asn Leu Val Asn 
        275                 280                 285             


Met Arg Gly Ala Val Val Ile Asn Pro Pro Glu Ala Thr Gln Ser Val 
    290                 295                 300                 


Thr Glu Ser Thr His Asn Leu Thr Arg Asn Phe Pro Ala Asn Leu Phe 
305                 310                 315                 320 


Asn Ile Tyr Ser Asp Ile Glu Lys Ser Gln Ile Leu His Lys Gly Pro 
                325                 330                 335     


Trp Gly His Glu Asn Pro Gln Ile Gln Pro Ser Val His Ile Gly Ile 
            340                 345                 350         


Gln Ala Val Pro Ala Leu Thr Thr Gly Ala Leu Leu Val Asn Ser Ser 
        355                 360                 365             


Pro Leu Asn Ser Trp Thr Asp Ser Met Gly Tyr Ile Asp Val Met Ser 
    370                 375                 380                 


Ser Cys Thr Val Met Glu Ser Gln Pro Thr His Phe Pro Phe Ser Thr 
385                 390                 395                 400 


Asp Ala Asn Thr Asn Pro Gly Asn Thr Ile Tyr Arg Ile Asn Leu Thr 
                405                 410                 415     


Pro Asn Ser Leu Thr Ser Ala Phe Asn Gly Leu Tyr Gly Asn Gly Ala 
            420                 425                 430         


Thr Leu Gly Asn Val Gly Asp Asp Ala Pro Pro Ser Pro Gly Pro Asp 
        435                 440                 445             


Pro Gly Pro Gln Pro Pro Pro Pro Pro Pro Pro Ser Pro Thr Pro Val 
    450                 455                 460                 


Gly Met Val Ser Lys Gly Glu Glu Leu Phe Thr Gly Val Val Pro Ile 
465                 470                 475                 480 


Leu Val Glu Leu Asp Gly Asp Val Asn Gly His Lys Phe Ser Val Ser 
                485                 490                 495     


Gly Glu Gly Glu Gly Asp Ala Thr Tyr Gly Lys Leu Thr Leu Lys Phe 
            500                 505                 510         


Ile Cys Thr Thr Gly Lys Leu Pro Val Pro Trp Pro Thr Leu Val Thr 
        515                 520                 525             


Thr Phe Thr Tyr Gly Val Gln Cys Phe Ser Arg Tyr Pro Asp His Met 
    530                 535                 540                 


Lys Gln His Asp Phe Phe Lys Ser Ala Met Pro Glu Gly Tyr Val Gln 
545                 550                 555                 560 


Glu Arg Thr Ile Phe Phe Lys Asp Asp Gly Asn Tyr Lys Thr Arg Ala 
                565                 570                 575     


Glu Val Lys Phe Glu Gly Asp Thr Leu Val Asn Arg Ile Glu Leu Lys 
            580                 585                 590         


Gly Ile Asp Phe Lys Glu Asp Gly Asn Ile Leu Gly His Lys Leu Glu 
        595                 600                 605             


Tyr Asn Tyr Asn Ser His Asn Val Tyr Ile Met Ala Asp Lys Gln Lys 
    610                 615                 620                 


Asn Gly Ile Lys Val Asn Phe Lys Ile Arg His Asn Ile Glu Asp Gly 
625                 630                 635                 640 


Ser Val Gln Leu Ala Asp His Tyr Gln Gln Asn Thr Pro Ile Gly Asp 
                645                 650                 655     


Gly Pro Val Leu Leu Pro Asp Asn His Tyr Leu Ser Thr Gln Ser Ala 
            660                 665                 670         


Leu Ser Lys Asp Pro Asn Glu Lys Arg Asp His Met Val Leu Leu Glu 
        675                 680                 685             


Phe Val Thr Ala Ala Gly Ile Thr His Gly Met Asp Glu Leu Tyr Lys 
    690                 695                 700                 


<210>  4
<211>  32
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  peptide linker

<400>  4

Gly Asp Asp Ala Pro Pro Ser Pro Gly Pro Asp Pro Gly Pro Gln Pro 
1               5                   10                  15      


Pro Pro Pro Pro Pro Pro Ser Pro Thr Pro Val Gly Gly Ala Ala Gly 
            20                  25                  30          


<210>  5
<211>  6
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  artificial peptide sequence of an affinity tag

<400>  5

His His His His His His 
1               5       


<210>  6
<211>  37
<212>  PRT
<213>  Hadronyche versuta

<400>  6

Ser Pro Thr Cys Ile Pro Ser Gly Gln Pro Cys Pro Tyr Asn Glu Asn 
1               5                   10                  15      


Cys Cys Ser Gln Ser Cys Thr Phe Lys Glu Asn Glu Asn Gly Asn Thr 
            20                  25                  30          


Val Lys Arg Cys Asp 
        35          


<210>  7
<211>  88
<212>  PRT
<213>  Androctonus australis

<400>  7

Met Lys Phe Leu Leu Leu Phe Leu Val Val Leu Pro Ile Met Gly Val 
1               5                   10                  15      


Phe Gly Lys Lys Asn Gly Tyr Ala Val Asp Ser Ser Gly Lys Ala Pro 
            20                  25                  30          


Glu Cys Leu Leu Ser Asn Tyr Cys Asn Asn Glu Cys Thr Lys Val His 
        35                  40                  45              


Tyr Ala Asp Lys Gly Tyr Cys Cys Leu Leu Ser Cys Tyr Cys Phe Gly 
    50                  55                  60                  


Leu Asn Asp Asp Lys Lys Val Leu Glu Ile Ser Asp Thr Arg Lys Ser 
65                  70                  75                  80  


Tyr Cys Asp Thr Thr Ile Ile Asn 
                85              


<210>  8
<211>  88
<212>  PRT
<213>  Androctonus australis

<400>  8

Met Lys Phe Leu Leu Leu Phe Leu Val Val Leu Pro Ile Met Gly Val 
1               5                   10                  15      


Leu Gly Lys Lys Asn Gly Tyr Ala Val Asp Ser Ser Gly Lys Ala Pro 
            20                  25                  30          


Glu Cys Leu Leu Ser Asn Tyr Cys Tyr Asn Glu Cys Thr Lys Val His 
        35                  40                  45              


Tyr Ala Asp Lys Gly Tyr Cys Cys Leu Leu Ser Cys Tyr Cys Phe Gly 
    50                  55                  60                  


Leu Asn Asp Asp Lys Lys Val Leu Glu Ile Ser Asp Thr Arg Lys Ser 
65                  70                  75                  80  


Tyr Cys Asp Thr Pro Ile Ile Asn 
                85              


<210>  9
<211>  65
<212>  PRT
<213>  Androctonus australis

<400>  9

Glu His Gly Tyr Leu Leu Asn Lys Tyr Thr Gly Cys Lys Val Trp Cys 
1               5                   10                  15      


Val Ile Asn Asn Glu Glu Cys Gly Tyr Leu Cys Asn Lys Arg Arg Gly 
            20                  25                  30          


Gly Tyr Tyr Gly Tyr Cys Tyr Phe Trp Lys Leu Ala Cys Tyr Cys Gln 
        35                  40                  45              


Gly Ala Arg Lys Ser Glu Leu Trp Asn Tyr Lys Thr Asn Lys Cys Asp 
    50                  55                  60                  


Leu 
65  


<210>  10
<211>  60
<212>  PRT
<213>  Androctonus australis

<400>  10

Asp Gly Tyr Ile Lys Arg His Asp Gly Cys Lys Val Thr Cys Leu Ile 
1               5                   10                  15      


Asn Asp Asn Tyr Cys Asp Thr Glu Cys Lys Arg Glu Gly Gly Ser Tyr 
            20                  25                  30          


Gly Tyr Cys Tyr Ser Val Gly Phe Ala Cys Trp Cys Glu Gly Leu Pro 
        35                  40                  45              


Asp Asp Lys Ala Trp Lys Ser Glu Thr Asn Thr Cys 
    50                  55                  60  


<210>  11
<211>  64
<212>  PRT
<213>  Leiurus quinquestriatus

<400>  11

Asp Gly Tyr Ile Lys Arg Arg Asp Gly Cys Lys Val Ala Cys Leu Ile 
1               5                   10                  15      


Gly Asn Glu Gly Cys Asp Lys Glu Cys Lys Ala Tyr Gly Gly Ser Tyr 
            20                  25                  30          


Gly Tyr Cys Trp Thr Trp Gly Leu Ala Cys Trp Cys Glu Gly Leu Pro 
        35                  40                  45              


Asp Asp Lys Thr Trp Lys Ser Glu Thr Asn Thr Cys Gly Gly Lys Lys 
    50                  55                  60                  


<210>  12
<211>  61
<212>  PRT
<213>  Leiurus quinquestriatus

<400>  12

Asp Gly Tyr Ile Arg Lys Arg Asp Gly Cys Lys Leu Ser Cys Leu Phe 
1               5                   10                  15      


Gly Asn Glu Gly Cys Asn Lys Glu Cys Lys Ser Tyr Gly Gly Ser Tyr 
            20                  25                  30          


Gly Tyr Cys Trp Thr Trp Gly Leu Ala Cys Trp Cys Glu Gly Leu Pro 
        35                  40                  45              


Asp Asp Lys Thr Trp Lys Ser Glu Thr Asn Thr Cys Gly 
    50                  55                  60      


<210>  13
<211>  2124
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  recombinant DNA sequence encoding Junonia coenia Densovirus coat 
       protein VP4 fused to eGFP via a peptide linker

<400>  13
atgtcattac ctggaactgg ttctggaaca tcatctggag gaggcaacac ttcaggtcaa       60

gaggtttatg taattcctcg tccattttcg aactttggta aaaaattaag tacttataca      120

aagtctcata aatttatgat atttggtctt gccaataatg ttattggacc tacaggtact      180

ggtacaacag ctgtaaatcg tttaattaca acttgtttgg ctgaaattcc atggcagaaa      240

ttgcctttgt atatgaacca atctgaattt gatttattac ctcctggtag tagagtagtt      300

gaatgtaatg ttaaagtaat attcagaact aatcgtattg catttgagac tagttcaact      360

gctactaaac aagctacatt gaatcaaata tctaatttac aaactgctgt tggattaaat      420

aaacttggat ggggtattga tagatcattt actgcttttc aatcagatca acctatgatt      480

cccactgcta ctagtgcacc aaaatatgaa cctataactg gtacgactgg ttatagaggt      540

atgatagctg attattatgg tgctgattct actaatgatg ctgcatttgg taatgctggt      600

aactatcctc atcatcaagt tggttcattt acttttattc aaaattatta ttgtatgtat      660

caacaaacca atcaaggtac tggaggttgg ccatgtttag ctgaacatct tcaacaattt      720

gattctaaaa ctgttaataa tcaatgttta attgatgtaa cttataaacc taaaatgggt      780

ttaattaaac caccgttaaa ttataaaatt attggtcaac ctactgcaaa aggtactata      840

tctgttggtg ataatttagt taacatgcga ggagctgttg taataaatcc acctgaagca      900

acacaatctg ttactgaatc aactcataat ttgactcgca attttccagc taatttgttt      960

aatatttatt ctgacattga aaaatctcaa attttacata aaggaccttg gggacacgaa     1020

aatccacaga tacaaccaag tgttcatatt ggtattcaag ctgtaccagc attaactaca     1080

ggagctttac ttgtaaattc aagtccttta aattcatgga ctgattctat gggttatatt     1140

gatgttatgt ctagttgtac tgttatggaa tctcagccta cacactttcc attttcgact     1200

gatgctaata ctaaccctgg taataccatt tatcgtatta atcttacacc gaactctctt     1260

actagtgctt tcaatggatt gtacggtaat ggagctactc ttggtaacgt tggggacgac     1320

gctcccccgt caccagggcc tgatcccggg ccccaaccac caccacctcc acccccaagt     1380

cccactcccg taggaatggt gagcaagggc gaggagctgt tcaccggggt ggtgcccatc     1440

ctggtcgagc tggacggcga cgtaaacggc cacaagttca gcgtgtccgg cgagggcgag     1500

ggcgatgcca cctacggcaa gctgaccctg aagttcatct gcaccaccgg caagctgccc     1560

gtgccctggc ccaccctcgt gaccaccttc acctacggcg tgcagtgctt cagccgctac     1620

cccgaccaca tgaagcagca cgacttcttc aagtccgcca tgcccgaagg ctacgtccag     1680

gagcgcacca tcttcttcaa ggacgacggc aactacaaga cccgcgccga ggtgaagttc     1740

gagggcgaca ccctggtgaa ccgcatcgag ctgaagggca tcgacttcaa ggaggacggc     1800

aacatcctgg ggcacaagct ggagtacaac tacaacagcc acaacgtcta tatcatggcc     1860

gacaagcaga agaacggcat caaggtgaac ttcaagatcc gccacaacat cgaggacggc     1920

agcgtgcagc tcgccgacca ctaccagcag aacaccccca tcggcgacgg ccccgtgctg     1980

ctgcccgaca accactacct gagcacccag tccgccctga gcaaagaccc caacgagaag     2040

cgcgatcaca tggtcctgct ggagttcgtg accgccgccg ggatcactca cggcatggac     2100

gagctgtaca agtaaagcgg ctaa                                            2124


<210>  14
<211>  84
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  DNA sequence for PolyProline Linker

<400>  14
ggggacgacg ctcccccgtc accagggcct gatcccgggc cccaaccacc accacctcca       60

cccccaagtc ccactcccgt agga                                              84


<210>  15
<211>  533
<212>  PRT
<213>  Junonia coenia


<220>
<221>  misc_feature
<222>  (91)..(91)
<223>  Xaa can be any naturally occurring amino acid

<400>  15

Met Ser Arg Gln Ile Asn Ser Lys Tyr Leu Asn Ser Trp His Asp Trp 
1               5                   10                  15      


Ile Glu Gln Asn Lys His Asn Asn Phe Glu Gly Ile Gln Leu Pro Glu 
            20                  25                  30          


Asp Phe Tyr Thr Glu Glu Gln Thr Leu Ser Asp Ser Pro Met Ser Glu 
        35                  40                  45              


Gly Thr Lys Arg Lys Ala Asp Thr Pro Val Glu Glu Gly Pro Ser Lys 
    50                  55                  60                  


Lys Gly Ala His Asn Ala Pro His Asn Ser Gln Gly Thr Asp Pro Gln 
65                  70                  75                  80  


Asn Pro Ser Ser Ser Gly Ala Thr Thr Ser Xaa Asp Val Glu Met Ala 
                85                  90                  95      


Met Ser Leu Pro Gly Thr Gly Ser Gly Thr Ser Ser Gly Gly Gly Asn 
            100                 105                 110         


Thr Ser Gly Gln Glu Val Tyr Val Ile Pro Arg Pro Phe Ser Asn Phe 
        115                 120                 125             


Gly Lys Lys Leu Ser Thr Tyr Thr Lys Ser His Lys Phe Met Ile Phe 
    130                 135                 140                 


Gly Leu Ala Asn Asn Val Ile Gly Pro Thr Gly Thr Gly Thr Thr Ala 
145                 150                 155                 160 


Val Asn Arg Leu Ile Thr Thr Cys Leu Ala Glu Ile Pro Trp Gln Lys 
                165                 170                 175     


Leu Pro Leu Tyr Met Asn Gln Ser Glu Phe Asp Leu Leu Pro Pro Gly 
            180                 185                 190         


Ser Arg Val Val Glu Cys Asn Val Lys Val Ile Phe Arg Thr Asn Arg 
        195                 200                 205             


Ile Ala Phe Glu Thr Ser Ser Thr Ala Thr Lys Gln Ala Thr Leu Asn 
    210                 215                 220                 


Gln Ile Ser Asn Leu Gln Thr Ala Val Gly Leu Asn Lys Leu Gly Trp 
225                 230                 235                 240 


Gly Ile Asp Arg Ser Phe Thr Ala Phe Gln Ser Asp Gln Pro Met Ile 
                245                 250                 255     


Pro Thr Ala Thr Ser Ala Pro Lys Tyr Glu Pro Ile Thr Gly Thr Thr 
            260                 265                 270         


Gly Tyr Arg Gly Met Ile Ala Asp Tyr Tyr Gly Ala Asp Ser Thr Asn 
        275                 280                 285             


Asp Ala Ala Phe Gly Asn Ala Gly Asn Tyr Pro His His Gln Val Gly 
    290                 295                 300                 


Ser Phe Thr Phe Ile Gln Asn Tyr Tyr Cys Met Tyr Gln Gln Thr Asn 
305                 310                 315                 320 


Gln Gly Thr Gly Gly Trp Pro Cys Leu Ala Glu His Leu Gln Gln Phe 
                325                 330                 335     


Asp Ser Lys Thr Val Asn Asn Gln Cys Leu Ile Asp Val Thr Tyr Lys 
            340                 345                 350         


Pro Lys Met Gly Leu Ile Lys Pro Pro Leu Asn Tyr Lys Ile Ile Gly 
        355                 360                 365             


Gln Pro Thr Ala Lys Gly Thr Ile Ser Val Gly Asp Asn Leu Val Asn 
    370                 375                 380                 


Met Arg Gly Ala Val Val Ile Asn Pro Pro Glu Ala Thr Gln Ser Val 
385                 390                 395                 400 


Thr Glu Ser Thr His Asn Leu Thr Arg Asn Phe Pro Ala Asn Leu Phe 
                405                 410                 415     


Asn Ile Tyr Ser Asp Ile Glu Lys Ser Gln Ile Leu His Lys Gly Pro 
            420                 425                 430         


Trp Gly His Glu Asn Pro Gln Ile Gln Pro Ser Val His Ile Gly Ile 
        435                 440                 445             


Gln Ala Val Pro Ala Leu Thr Thr Gly Ala Leu Leu Val Asn Ser Ser 
    450                 455                 460                 


Pro Leu Asn Ser Trp Thr Asp Ser Met Gly Tyr Ile Asp Val Met Ser 
465                 470                 475                 480 


Ser Cys Thr Val Met Glu Ser Gln Pro Thr His Phe Pro Phe Ser Thr 
                485                 490                 495     


Asp Ala Asn Thr Asn Pro Gly Asn Thr Ile Tyr Arg Ile Asn Leu Thr 
            500                 505                 510         


Pro Asn Ser Leu Thr Ser Ala Phe Asn Gly Leu Tyr Gly Asn Gly Ala 
        515                 520                 525             


Thr Leu Gly Asn Val 
    530             


<210>  16
<211>  488
<212>  PRT
<213>  Junonia coenia


<220>
<221>  misc_feature
<222>  (46)..(46)
<223>  Xaa can be any naturally occurring amino acid

<400>  16

Met Ser Glu Gly Thr Lys Arg Lys Ala Asp Thr Pro Val Glu Glu Gly 
1               5                   10                  15      


Pro Ser Lys Lys Gly Ala His Asn Ala Pro His Asn Ser Gln Gly Thr 
            20                  25                  30          


Asp Pro Gln Asn Pro Ser Ser Ser Gly Ala Thr Thr Ser Xaa Asp Val 
        35                  40                  45              


Glu Met Ala Met Ser Leu Pro Gly Thr Gly Ser Gly Thr Ser Ser Gly 
    50                  55                  60                  


Gly Gly Asn Thr Ser Gly Gln Glu Val Tyr Val Ile Pro Arg Pro Phe 
65                  70                  75                  80  


Ser Asn Phe Gly Lys Lys Leu Ser Thr Tyr Thr Lys Ser His Lys Phe 
                85                  90                  95      


Met Ile Phe Gly Leu Ala Asn Asn Val Ile Gly Pro Thr Gly Thr Gly 
            100                 105                 110         


Thr Thr Ala Val Asn Arg Leu Ile Thr Thr Cys Leu Ala Glu Ile Pro 
        115                 120                 125             


Trp Gln Lys Leu Pro Leu Tyr Met Asn Gln Ser Glu Phe Asp Leu Leu 
    130                 135                 140                 


Pro Pro Gly Ser Arg Val Val Glu Cys Asn Val Lys Val Ile Phe Arg 
145                 150                 155                 160 


Thr Asn Arg Ile Ala Phe Glu Thr Ser Ser Thr Ala Thr Lys Gln Ala 
                165                 170                 175     


Thr Leu Asn Gln Ile Ser Asn Leu Gln Thr Ala Val Gly Leu Asn Lys 
            180                 185                 190         


Leu Gly Trp Gly Ile Asp Arg Ser Phe Thr Ala Phe Gln Ser Asp Gln 
        195                 200                 205             


Pro Met Ile Pro Thr Ala Thr Ser Ala Pro Lys Tyr Glu Pro Ile Thr 
    210                 215                 220                 


Gly Thr Thr Gly Tyr Arg Gly Met Ile Ala Asp Tyr Tyr Gly Ala Asp 
225                 230                 235                 240 


Ser Thr Asn Asp Ala Ala Phe Gly Asn Ala Gly Asn Tyr Pro His His 
                245                 250                 255     


Gln Val Gly Ser Phe Thr Phe Ile Gln Asn Tyr Tyr Cys Met Tyr Gln 
            260                 265                 270         


Gln Thr Asn Gln Gly Thr Gly Gly Trp Pro Cys Leu Ala Glu His Leu 
        275                 280                 285             


Gln Gln Phe Asp Ser Lys Thr Val Asn Asn Gln Cys Leu Ile Asp Val 
    290                 295                 300                 


Thr Tyr Lys Pro Lys Met Gly Leu Ile Lys Pro Pro Leu Asn Tyr Lys 
305                 310                 315                 320 


Ile Ile Gly Gln Pro Thr Ala Lys Gly Thr Ile Ser Val Gly Asp Asn 
                325                 330                 335     


Leu Val Asn Met Arg Gly Ala Val Val Ile Asn Pro Pro Glu Ala Thr 
            340                 345                 350         


Gln Ser Val Thr Glu Ser Thr His Asn Leu Thr Arg Asn Phe Pro Ala 
        355                 360                 365             


Asn Leu Phe Asn Ile Tyr Ser Asp Ile Glu Lys Ser Gln Ile Leu His 
    370                 375                 380                 


Lys Gly Pro Trp Gly His Glu Asn Pro Gln Ile Gln Pro Ser Val His 
385                 390                 395                 400 


Ile Gly Ile Gln Ala Val Pro Ala Leu Thr Thr Gly Ala Leu Leu Val 
                405                 410                 415     


Asn Ser Ser Pro Leu Asn Ser Trp Thr Asp Ser Met Gly Tyr Ile Asp 
            420                 425                 430         


Val Met Ser Ser Cys Thr Val Met Glu Ser Gln Pro Thr His Phe Pro 
        435                 440                 445             


Phe Ser Thr Asp Ala Asn Thr Asn Pro Gly Asn Thr Ile Tyr Arg Ile 
    450                 455                 460                 


Asn Leu Thr Pro Asn Ser Leu Thr Ser Ala Phe Asn Gly Leu Tyr Gly 
465                 470                 475                 480 


Asn Gly Ala Thr Leu Gly Asn Val 
                485             


