                         SEQUENCE LISTING

<110>  SANOFI PASTEUR
 
<120>  LIVE ATTENUATED CHIMERIC ZIKA VIRUS AND ITS USE AS AN IMMUNOGENIC
        COMPOSITION

<130>  B12091B CS

<150>  US 62/402,640
<151>  2016-09-30

<150>  US 62/524,193
<151>  2017-06-23

<160>  13    

<170>  PatentIn version 3.5

<210>  1
<211>  2016
<212>  RNA
<213>  Zika virus

<400>  1
gcggagguca cuagacgugg gagugcauac uauauguacu uggacagaaa cgacgcuggg       60

gaggccauau cuuuuccaac cacauugggg augaauaagu guuauauaca gaucauggau      120

cuuggacaca ugugugaugc caccaugagc uaugaaugcc cuaugcugga ugagggggug      180

gaaccagaug acgucgauug uuggugcaac acgacgucaa cuuggguugu guacggaacc      240

ugccaucaca aaaaagguga agcacggaga ucuagaagag cugugacgcu ccccucccau      300

uccacuagga agcugcaaac gcggucgcaa accugguugg aaucaagaga auacacaaag      360

cacuugauua gagucgaaaa uuggauauuc aggaacccug gcuucgcguu agcagcagcu      420

gccaucgcuu ggcuuuuggg aagcucaacg agccaaaaag ucauauacuu ggucaugaua      480

cugcugauug ccccggcaua cagcaucagg ugcauaggag ucagcaauag ggacuuugug      540

gaagguaugu caggugggac uuggguugau guugucuugg aacauggagg uugugucacc      600

guaauggcac aggacaaacc gacugucgac auagagcugg uuacaacaac agucagcaac      660

auggcggagg uaagauccua cugcuaugag gcaucaauau cggacauggc uucggacagc      720

cgcugcccaa cacaagguga agccuaccuu gacaagcaau cagacacuca auaugucugc      780

aaaagaacgu uaguggacag aggcugggga aauggaugug gacuuuuugg caaagggagc      840

cuggugacau gcgcuaaguu ugcaugcucc aagaaaauga ccgggaagag cauccagcca      900

gagaaucugg aguaccggau aaugcuguca guucauggcu cccagcacag ugggaugauc      960

guuaaugaca caggacauga aacugaugag aauagagcga agguugagau aacgcccaau     1020

ucaccaagag ccgaagccac ccuggggggu uuuggaagcc uaggacuuga uugugaaccg     1080

aggacaggcc uugacuuuuc agauuuguau uacuugacua ugaauaacaa gcacugguug     1140

guucacaagg agugguucca cgacauucca uuaccuuggc acgcuggggc agacaccgga     1200

acuccacacu ggaacaacaa agaagcacug guagaguuca aggacgcaca ugccaaaagg     1260

caaacugucg ugguucuagg gagucaagaa ggagcaguuc acacggcccu ugcuggagcu     1320

cuggaggcug agauggaugg ugcaaaggga aggcuguccu cuggccacuu gaaaugucgc     1380

cugaaaaugg auaaacuuag auugaagggc gugucauacu ccuuguguac cgcagcguuc     1440

acauucacca agaucccggc ugaaacacug cacgggacag ucacagugga gguacaguac     1500

gcagggacag auggaccuug caagguucca gcucagaugg cgguggacau gcaaacucug     1560

accccaguug ggagguugau aaccgcuaac cccguaauca cugaaagcac ugagaacucu     1620

aagaugaugc uggaacuuga uccaccauuu ggggacucuu acauugucau aggagucggg     1680

gagaagaaga ucacccacca cuggcacagg aguggcagca ccauuggaaa agcauuugaa     1740

gccacuguga gaggugccaa gagaauggca gucuugggag acacagccug ggacuuugga     1800

ucaguuggag gcgcucucaa cucauugggc aagggcaucc aucaaauuuu uggagcagcu     1860

uucaaaucau uguuuggagg aauguccugg uucucacaaa uucucauugg aacguugcug     1920

augugguugg gucugaacac aaagaaugga ucuauuuccc uuaugugcuu ggccuuaggg     1980

ggaguguuga ucuucuuauc cacagcuguc ucugcu                               2016


<210>  2
<211>  672
<212>  PRT
<213>  Zika virus

<400>  2

Ala Glu Val Thr Arg Arg Gly Ser Ala Tyr Tyr Met Tyr Leu Asp Arg 
1               5                   10                  15      


Asn Asp Ala Gly Glu Ala Ile Ser Phe Pro Thr Thr Leu Gly Met Asn 
            20                  25                  30          


Lys Cys Tyr Ile Gln Ile Met Asp Leu Gly His Met Cys Asp Ala Thr 
        35                  40                  45              


Met Ser Tyr Glu Cys Pro Met Leu Asp Glu Gly Val Glu Pro Asp Asp 
    50                  55                  60                  


Val Asp Cys Trp Cys Asn Thr Thr Ser Thr Trp Val Val Tyr Gly Thr 
65                  70                  75                  80  


Cys His His Lys Lys Gly Glu Ala Arg Arg Ser Arg Arg Ala Val Thr 
                85                  90                  95      


Leu Pro Ser His Ser Thr Arg Lys Leu Gln Thr Arg Ser Gln Thr Trp 
            100                 105                 110         


Leu Glu Ser Arg Glu Tyr Thr Lys His Leu Ile Arg Val Glu Asn Trp 
        115                 120                 125             


Ile Phe Arg Asn Pro Gly Phe Ala Leu Ala Ala Ala Ala Ile Ala Trp 
    130                 135                 140                 


Leu Leu Gly Ser Ser Thr Ser Gln Lys Val Ile Tyr Leu Val Met Ile 
145                 150                 155                 160 


Leu Leu Ile Ala Pro Ala Tyr Ser Ile Arg Cys Ile Gly Val Ser Asn 
                165                 170                 175     


Arg Asp Phe Val Glu Gly Met Ser Gly Gly Thr Trp Val Asp Val Val 
            180                 185                 190         


Leu Glu His Gly Gly Cys Val Thr Val Met Ala Gln Asp Lys Pro Thr 
        195                 200                 205             


Val Asp Ile Glu Leu Val Thr Thr Thr Val Ser Asn Met Ala Glu Val 
    210                 215                 220                 


Arg Ser Tyr Cys Tyr Glu Ala Ser Ile Ser Asp Met Ala Ser Asp Ser 
225                 230                 235                 240 


Arg Cys Pro Thr Gln Gly Glu Ala Tyr Leu Asp Lys Gln Ser Asp Thr 
                245                 250                 255     


Gln Tyr Val Cys Lys Arg Thr Leu Val Asp Arg Gly Trp Gly Asn Gly 
            260                 265                 270         


Cys Gly Leu Phe Gly Lys Gly Ser Leu Val Thr Cys Ala Lys Phe Ala 
        275                 280                 285             


Cys Ser Lys Lys Met Thr Gly Lys Ser Ile Gln Pro Glu Asn Leu Glu 
    290                 295                 300                 


Tyr Arg Ile Met Leu Ser Val His Gly Ser Gln His Ser Gly Met Ile 
305                 310                 315                 320 


Val Asn Asp Thr Gly His Glu Thr Asp Glu Asn Arg Ala Lys Val Glu 
                325                 330                 335     


Ile Thr Pro Asn Ser Pro Arg Ala Glu Ala Thr Leu Gly Gly Phe Gly 
            340                 345                 350         


Ser Leu Gly Leu Asp Cys Glu Pro Arg Thr Gly Leu Asp Phe Ser Asp 
        355                 360                 365             


Leu Tyr Tyr Leu Thr Met Asn Asn Lys His Trp Leu Val His Lys Glu 
    370                 375                 380                 


Trp Phe His Asp Ile Pro Leu Pro Trp His Ala Gly Ala Asp Thr Gly 
385                 390                 395                 400 


Thr Pro His Trp Asn Asn Lys Glu Ala Leu Val Glu Phe Lys Asp Ala 
                405                 410                 415     


His Ala Lys Arg Gln Thr Val Val Val Leu Gly Ser Gln Glu Gly Ala 
            420                 425                 430         


Val His Thr Ala Leu Ala Gly Ala Leu Glu Ala Glu Met Asp Gly Ala 
        435                 440                 445             


Lys Gly Arg Leu Ser Ser Gly His Leu Lys Cys Arg Leu Lys Met Asp 
    450                 455                 460                 


Lys Leu Arg Leu Lys Gly Val Ser Tyr Ser Leu Cys Thr Ala Ala Phe 
465                 470                 475                 480 


Thr Phe Thr Lys Ile Pro Ala Glu Thr Leu His Gly Thr Val Thr Val 
                485                 490                 495     


Glu Val Gln Tyr Ala Gly Thr Asp Gly Pro Cys Lys Val Pro Ala Gln 
            500                 505                 510         


Met Ala Val Asp Met Gln Thr Leu Thr Pro Val Gly Arg Leu Ile Thr 
        515                 520                 525             


Ala Asn Pro Val Ile Thr Glu Ser Thr Glu Asn Ser Lys Met Met Leu 
    530                 535                 540                 


Glu Leu Asp Pro Pro Phe Gly Asp Ser Tyr Ile Val Ile Gly Val Gly 
545                 550                 555                 560 


Glu Lys Lys Ile Thr His His Trp His Arg Ser Gly Ser Thr Ile Gly 
                565                 570                 575     


Lys Ala Phe Glu Ala Thr Val Arg Gly Ala Lys Arg Met Ala Val Leu 
            580                 585                 590         


Gly Asp Thr Ala Trp Asp Phe Gly Ser Val Gly Gly Ala Leu Asn Ser 
        595                 600                 605             


Leu Gly Lys Gly Ile His Gln Ile Phe Gly Ala Ala Phe Lys Ser Leu 
    610                 615                 620                 


Phe Gly Gly Met Ser Trp Phe Ser Gln Ile Leu Ile Gly Thr Leu Leu 
625                 630                 635                 640 


Met Trp Leu Gly Leu Asn Thr Lys Asn Gly Ser Ile Ser Leu Met Cys 
                645                 650                 655     


Leu Ala Leu Gly Gly Val Leu Ile Phe Leu Ser Thr Ala Val Ser Ala 
            660                 665                 670         


<210>  3
<211>  168
<212>  PRT
<213>  Zika virus

<400>  3

Ala Glu Val Thr Arg Arg Gly Ser Ala Tyr Tyr Met Tyr Leu Asp Arg 
1               5                   10                  15      


Asn Asp Ala Gly Glu Ala Ile Ser Phe Pro Thr Thr Leu Gly Met Asn 
            20                  25                  30          


Lys Cys Tyr Ile Gln Ile Met Asp Leu Gly His Met Cys Asp Ala Thr 
        35                  40                  45              


Met Ser Tyr Glu Cys Pro Met Leu Asp Glu Gly Val Glu Pro Asp Asp 
    50                  55                  60                  


Val Asp Cys Trp Cys Asn Thr Thr Ser Thr Trp Val Val Tyr Gly Thr 
65                  70                  75                  80  


Cys His His Lys Lys Gly Glu Ala Arg Arg Ser Arg Arg Ala Val Thr 
                85                  90                  95      


Leu Pro Ser His Ser Thr Arg Lys Leu Gln Thr Arg Ser Gln Thr Trp 
            100                 105                 110         


Leu Glu Ser Arg Glu Tyr Thr Lys His Leu Ile Arg Val Glu Asn Trp 
        115                 120                 125             


Ile Phe Arg Asn Pro Gly Phe Ala Leu Ala Ala Ala Ala Ile Ala Trp 
    130                 135                 140                 


Leu Leu Gly Ser Ser Thr Ser Gln Lys Val Ile Tyr Leu Val Met Ile 
145                 150                 155                 160 


Leu Leu Ile Ala Pro Ala Tyr Ser 
                165             


<210>  4
<211>  504
<212>  PRT
<213>  Zika virus

<400>  4

Ile Arg Cys Ile Gly Val Ser Asn Arg Asp Phe Val Glu Gly Met Ser 
1               5                   10                  15      


Gly Gly Thr Trp Val Asp Val Val Leu Glu His Gly Gly Cys Val Thr 
            20                  25                  30          


Val Met Ala Gln Asp Lys Pro Thr Val Asp Ile Glu Leu Val Thr Thr 
        35                  40                  45              


Thr Val Ser Asn Met Ala Glu Val Arg Ser Tyr Cys Tyr Glu Ala Ser 
    50                  55                  60                  


Ile Ser Asp Met Ala Ser Asp Ser Arg Cys Pro Thr Gln Gly Glu Ala 
65                  70                  75                  80  


Tyr Leu Asp Lys Gln Ser Asp Thr Gln Tyr Val Cys Lys Arg Thr Leu 
                85                  90                  95      


Val Asp Arg Gly Trp Gly Asn Gly Cys Gly Leu Phe Gly Lys Gly Ser 
            100                 105                 110         


Leu Val Thr Cys Ala Lys Phe Ala Cys Ser Lys Lys Met Thr Gly Lys 
        115                 120                 125             


Ser Ile Gln Pro Glu Asn Leu Glu Tyr Arg Ile Met Leu Ser Val His 
    130                 135                 140                 


Gly Ser Gln His Ser Gly Met Ile Val Asn Asp Thr Gly His Glu Thr 
145                 150                 155                 160 


Asp Glu Asn Arg Ala Lys Val Glu Ile Thr Pro Asn Ser Pro Arg Ala 
                165                 170                 175     


Glu Ala Thr Leu Gly Gly Phe Gly Ser Leu Gly Leu Asp Cys Glu Pro 
            180                 185                 190         


Arg Thr Gly Leu Asp Phe Ser Asp Leu Tyr Tyr Leu Thr Met Asn Asn 
        195                 200                 205             


Lys His Trp Leu Val His Lys Glu Trp Phe His Asp Ile Pro Leu Pro 
    210                 215                 220                 


Trp His Ala Gly Ala Asp Thr Gly Thr Pro His Trp Asn Asn Lys Glu 
225                 230                 235                 240 


Ala Leu Val Glu Phe Lys Asp Ala His Ala Lys Arg Gln Thr Val Val 
                245                 250                 255     


Val Leu Gly Ser Gln Glu Gly Ala Val His Thr Ala Leu Ala Gly Ala 
            260                 265                 270         


Leu Glu Ala Glu Met Asp Gly Ala Lys Gly Arg Leu Ser Ser Gly His 
        275                 280                 285             


Leu Lys Cys Arg Leu Lys Met Asp Lys Leu Arg Leu Lys Gly Val Ser 
    290                 295                 300                 


Tyr Ser Leu Cys Thr Ala Ala Phe Thr Phe Thr Lys Ile Pro Ala Glu 
305                 310                 315                 320 


Thr Leu His Gly Thr Val Thr Val Glu Val Gln Tyr Ala Gly Thr Asp 
                325                 330                 335     


Gly Pro Cys Lys Val Pro Ala Gln Met Ala Val Asp Met Gln Thr Leu 
            340                 345                 350         


Thr Pro Val Gly Arg Leu Ile Thr Ala Asn Pro Val Ile Thr Glu Ser 
        355                 360                 365             


Thr Glu Asn Ser Lys Met Met Leu Glu Leu Asp Pro Pro Phe Gly Asp 
    370                 375                 380                 


Ser Tyr Ile Val Ile Gly Val Gly Glu Lys Lys Ile Thr His His Trp 
385                 390                 395                 400 


His Arg Ser Gly Ser Thr Ile Gly Lys Ala Phe Glu Ala Thr Val Arg 
                405                 410                 415     


Gly Ala Lys Arg Met Ala Val Leu Gly Asp Thr Ala Trp Asp Phe Gly 
            420                 425                 430         


Ser Val Gly Gly Ala Leu Asn Ser Leu Gly Lys Gly Ile His Gln Ile 
        435                 440                 445             


Phe Gly Ala Ala Phe Lys Ser Leu Phe Gly Gly Met Ser Trp Phe Ser 
    450                 455                 460                 


Gln Ile Leu Ile Gly Thr Leu Leu Met Trp Leu Gly Leu Asn Thr Lys 
465                 470                 475                 480 


Asn Gly Ser Ile Ser Leu Met Cys Leu Ala Leu Gly Gly Val Leu Ile 
                485                 490                 495     


Phe Leu Ser Thr Ala Val Ser Ala 
            500                 


<210>  5
<211>  75
<212>  PRT
<213>  Zika virus

<400>  5

Ala Val Thr Leu Pro Ser His Ser Thr Arg Lys Leu Gln Thr Arg Ser 
1               5                   10                  15      


Gln Thr Trp Leu Glu Ser Arg Glu Tyr Thr Lys His Leu Ile Arg Val 
            20                  25                  30          


Glu Asn Trp Ile Phe Arg Asn Pro Gly Phe Ala Leu Ala Ala Ala Ala 
        35                  40                  45              


Ile Ala Trp Leu Leu Gly Ser Ser Thr Ser Gln Lys Val Ile Tyr Leu 
    50                  55                  60                  


Val Met Ile Leu Leu Ile Ala Pro Ala Tyr Ser 
65                  70                  75  


<210>  6
<211>  20
<212>  PRT
<213>  Chimeric YFV/ZIKV

<400>  6

Ser His Asp Val Leu Thr Val Gln Phe Leu Ile Leu Gly Met Leu Leu 
1               5                   10                  15      


Thr Ala Met Ala 
            20  


<210>  7
<211>  101
<212>  PRT
<213>  Yellow fever virus

<400>  7

Met Ser Gly Arg Lys Ala Gln Gly Lys Thr Leu Gly Val Asn Met Val 
1               5                   10                  15      


Arg Arg Gly Val Arg Ser Leu Ser Asn Lys Ile Lys Gln Lys Thr Lys 
            20                  25                  30          


Gln Ile Gly Asn Arg Pro Gly Pro Ser Arg Gly Val Gln Gly Phe Ile 
        35                  40                  45              


Phe Phe Phe Leu Phe Asn Ile Leu Thr Gly Lys Lys Ile Thr Ala His 
    50                  55                  60                  


Leu Lys Arg Leu Trp Lys Met Leu Asp Pro Arg Gln Gly Leu Ala Val 
65                  70                  75                  80  


Leu Arg Lys Val Lys Arg Val Val Ala Ser Leu Met Arg Gly Leu Ser 
                85                  90                  95      


Ser Arg Lys Arg Arg 
            100     


<210>  8
<211>  511
<212>  RNA
<213>  Yellow fever virus

<400>  8
ugaaacacca ucuaacagga auaaccggga uacaaaccac ggguggagaa ccggacuccc       60

cacaaccuga aaccgggaua uaaaccacgg cuggagaacc ggacuccgca cuuaaaauga      120

aacagaaacc gggauaaaaa cuacggaugg agaaccggac uccacacauu gagacagaag      180

aaguugucag cccagaaccc cacacgaguu uugccacugc uaagcuguga ggcagugcag      240

gcugggacag ccgaccucca gguugcgaaa aaccugguuu cugggaccuc ccaccccaga      300

guaaaaagaa cggagccucc gcuaccaccc ucccacgugg ugguagaaag acggggucua      360

gagguuagag gagacccucc agggaacaaa uagugggacc auauugacgc cagggaaaga      420

ccggaguggu ucucugcuuu uccuccagag gucugugagc acaguuugcu caagaauaag      480

cagaccuuug gaugacaaac acaaaaccac u                                     511


<210>  9
<211>  506
<212>  RNA
<213>  Yellow fever virus

<400>  9
ugaaacacca ucuaacagga auaaccggga uacaaaccac ggguggagaa ccggacuccc       60

cacaaccuga aaccgggaua uaaaccacgg cuggagaacc ggacuccgca cuuaaaauga      120

aacagaaacc gggauaaaaa cuacggaugg agaaccggac uccacacauu gagacagaag      180

aaguugucag cccagaaccc cacacgaguu uugccacugc uaagcuguga ggcagugcag      240

gcugggacag ccgaccucug cgaaaaaccu gguuucuggg accucccacc ccagaguaaa      300

aagaacggag ccuccgcuac cacccuccca cgugguggua gaaagacggg gucuagaggu      360

uagaggagac ccuccaggga acaaauagug ggaccauauu gacgccaggg aaagaccgga      420

gugguucucu gcuuuuccuc cagaggucug ugagcacagu uugcucaaga auaagcagac      480

cuuuggauga caaacacaaa accacu                                           506


<210>  10
<211>  10907
<212>  DNA
<213>  Chimeric YFV/ZIKV

<400>  10
agtaaatcct gtgtgctaat tgaggtgcat tggtctgcaa atcgagttgc taggcaataa       60

acacatttgg attaatttta atcgttcgtt gagcgattag cagagaactg accagaacat      120

gtctggtcgt aaagctcagg gaaaaaccct gggcgtcaat atggtacgac gaggagttcg      180

ctccttgtca aacaaaataa aacaaaaaac aaaacaaatt ggaaacagac ctggaccttc      240

aagaggtgtt caaggattta tctttttctt tttgttcaac attttgactg gaaaaaagat      300

cacagcccac ctaaagaggt tgtggaaaat gctggaccca agacaaggct tggctgttct      360

aaggaaagtc aagagagtgg tggccagttt gatgagagga ttgtcctcaa ggaaacgccg      420

ttcccatgat gttctgactg tgcaattcct aattttgggc atgctgttga tgacgggtgg      480

agcggaggtc actagacgtg ggagtgcata ctatatgtac ttggacagaa acgacgctgg      540

ggaggccata tcttttccaa ccacattggg gatgaataag tgttatatac agatcatgga      600

tcttggacac atgtgtgatg ccaccatgag ctatgaatgc cctatgctgg atgagggggt      660

ggaaccagat gacgtcgatt gttggtgcaa cacgacgtca acttgggttg tgtacggaac      720

ctgccatcac aaaaaaggtg aagcacggag atctagaaga gctgtgacgc tcccctccca      780

ttccactagg aagctgcaaa cgcggtcgca aacctggttg gaatcaagag aatacacaaa      840

gcacttgatt agagtcgaaa attggatatt caggaaccct ggcttcgcgt tagcagcagc      900

tgccatcgct tggcttttgg gaagctcaac gagccaaaaa gtcatatact tggtcatgat      960

actgctgatt gccccggcat acagcatcag gtgcatagga gtcagcaata gggactttgt     1020

ggaaggtatg tcaggtggga cttgggttga tgttgtcttg gaacatggag gttgtgtcac     1080

cgtaatggca caggacaaac cgactgtcga catagagctg gttacaacaa cagtcagcaa     1140

catggcggag gtaagatcct actgctatga ggcatcaata tcggacatgg ctagcgacag     1200

ccgctgccca acacaaggtg aagcctacct tgacaagcaa tcagacactc aatatgtctg     1260

caaaagaacg ttagtggaca gaggctgggg aaatggatgt ggactttttg gcaaagggag     1320

cctggtgaca tgcgctaagt ttgcctgctc caagaaaatg accgggaaga gcatccagcc     1380

agagaatctg gagtaccgga taatgctgtc agttcatggc tcccagcaca gtgggatgat     1440

cgttaatgac acaggacatg aaactgatga gaatagagcg aaggttgaga taacgcccaa     1500

ttcaccaaga gccgaagcca ccctgggggg ttttggaagc ctaggacttg attgtgaacc     1560

gaggacaggc cttgactttt cagatttgta ttacttgact atgaataaca agcactggtt     1620

ggttcacaag gagtggttcc acgacattcc attaccttgg cacgctgggg cagacaccgg     1680

aactccacac tggaacaaca aagaagcact ggtagagttc aaggacgcac atgccaaaag     1740

gcaaactgtc gtggttctag ggagtcaaga aggagcagtt cacacggccc ttgctggagc     1800

tctggaggct gagatggatg gtgcaaaggg aaggctgtcc tctggccact tgaaatgtcg     1860

cctgaaaatg gataaactta gattgaaggg cgtgtcatac tccttgtgta ccgcagcgtt     1920

cacattcacc aagatcccgg ctgaaacact gcacgggaca gtcacagtgg aggtacagta     1980

cgcagggaca gatggacctt gcaaggttcc agctcagatg gcggtggaca tgcaaactct     2040

gaccccagtt gggaggttga taaccgctaa ccccgtaatc actgaaagca ctgagaactc     2100

taagatgatg ctggaacttg atccaccatt tggggactct tacattgtca taggagtcgg     2160

ggagaagaag atcacccacc actggcacag gagtggcagc accattggaa aagcatttga     2220

agccactgtg agaggtgcca agagaatggc agtcttggga gacacagcct gggactttgg     2280

atcagttgga ggcgctctca actcattggg caagggcatc catcaaattt ttggagcagc     2340

tttcaaatca ttgtttggag gaatgtcctg gttctcacaa attctcattg gaacgttgct     2400

gatgtggttg ggtttaaaca caaagaatgg atctatttcc cttatgtgct tggccttagg     2460

gggagtgttg atcttcttat ccacagctgt ctctgctgat caaggatgcg ccatcaactt     2520

tggcaagaga gagctcaagt gcggagatgg tatcttcata tttagagact ctgatgactg     2580

gctgaacaag tactcatact atccagaaga tcctgtgaag cttgcatcaa tagtgaaagc     2640

ctcttttgaa gaagggaagt gtggcctaaa ttcagttgac tcccttgagc atgagatgtg     2700

gagaagcagg gcagatgaga tcaatgccat ttttgaggaa aacgaggtgg acatttctgt     2760

tgtcgtgcag gatccaaaga atgtttacca gagaggaact catccatttt ccagaattcg     2820

ggatggtctg cagtatggtt ggaagacttg gggtaagaac cttgtgttct ccccagggag     2880

gaagaatgga agcttcatca tagatggaaa gtccaggaaa gaatgcccgt tttcaaaccg     2940

ggtctggaat tctttccaga tagaggagtt tgggacggga gtgttcacca cacgcgtgta     3000

catggacgca gtctttgaat acaccataga ctgcgatgga tctatcttgg gtgcagcggt     3060

gaacggaaaa aagagtgccc atggctctcc aacattttgg atgggaagtc atgaagtaaa     3120

tgggacatgg atgatccaca ccttggaggc attagattac aaggagtgtg agtggccact     3180

gacacatacg attggaacat cagttgaaga gagtgaaatg ttcatgccga gatcaatcgg     3240

aggcccagtt agctctcaca atcatatccc tggatacaag gttcagacga acggaccttg     3300

gatgcaggta ccactagaag tgaagagaga agcttgccca gggactagcg tgatcattga     3360

tggcaactgt gatggacggg gaaaatcaac cagatccacc acggatagcg ggaaagttat     3420

tcctgaatgg tgttgccgct cctgcacaat gccgcctgtg agcttccatg gtagtgatgg     3480

gtgttggtat cccatggaaa ttaggccaag gaaaacgcat gaaagccatc tggtgcgctc     3540

ctgggttacg gccggagaaa tacatgctgt cccttttggt ttggtgagca tgatgatagc     3600

aatggaagtg gtcctaagga aaagacaggg accaaagcaa atgttggttg gaggagtagt     3660

gctcttggga gcaatgctgg tcgggcaagt aactctcctt gatttgctga aactcacagt     3720

ggctgtggga ttgcatttcc atgagatgaa caatggagga gacgccatgt atatggcgtt     3780

gattgctgcc ttttcaatca gaccagggct gctcatcggc tttgggctca ggaccctatg     3840

gagccctcgg gaacgccttg tgctgaccct aggagcagcc atggtggaga ttgccttggg     3900

tggcgtgatg ggcggcctgt ggaagtatct aaatgcagtt tctctctgca tcctgacaat     3960

aaatgctgtt gcttctagga aagcatcaaa taccatcttg cccctcatgg ctctgttgac     4020

acctgtcact atggctgagg tgagacttgc cgcaatgttc ttttgtgcca tggttatcat     4080

aggggtcctt caccagaatt tcaaggacac ctccatgcag aagactatac ctctggtggc     4140

cctcacactc acatcttacc tgggcttgac acaacctttt ttgggcctgt gtgcatttct     4200

ggcaacccgc atatttgggc gaaggagtat cccagtgaat gaggcactcg cagcagctgg     4260

tctagtggga gtgctggcag gactggcttt tcaggagatg gagaacttcc ttggtccgat     4320

tgcagttgga ggactcctga tgatgctggt tagcgtggct gggagggtgg atgggctaga     4380

gctcaagaag cttggtgaag tttcatggga agaggaggcg gagatcagcg ggagttccgc     4440

ccgctatgat gtggcactca gtgaacaagg ggagttcaag ctgctttctg aagagaaagt     4500

gccatgggac caggttgtga tgacctcgct ggccttggtt ggggctgccc tccatccatt     4560

tgctcttctg ctggtccttg ctgggtggct gtttcatgtc aggggagcta ggagaagtgg     4620

ggatgtcttg tgggatattc ccactcctaa gatcatcgag gaatgtgaac atctggagga     4680

tgggatttat ggcatattcc agtcaacctt cttgggggcc tcccagcgag gagtgggagt     4740

ggcacaggga ggggtgttcc acacaatgtg gcatgtcaca agaggagctt tccttgtcag     4800

gaatggcaag aagttgattc catcttgggc ttcagtaaag gaagaccttg tcgcctatgg     4860

tggctcatgg aagttggaag gcagatggga tggagaggaa gaggtccagt tgatcgcggc     4920

tgttccagga aagaacgtgg tcaacgtcca gacaaaaccg agcttgttca aagtgaggaa     4980

tgggggagaa atcggggctg tcgctcttga ctatccgagt ggcacttcag gatctcctat     5040

tgttaacagg aacggagagg tgattgggct gtacggcaat ggcatccttg tcggtgacaa     5100

ctccttcgtg tccgccatat cccagactga ggtgaaggaa gaaggaaagg aggagctcca     5160

agagatcccg acaatgctaa agaaaggaat gacaactgtc cttgattttc atcctggagc     5220

tgggaagaca agacgtttcc tcccacagat cttggccgag tgcgcacgga gacgcttgcg     5280

cactcttgtg ttggccccca ccagggttgt tctttctgaa atgaaggagg cttttcacgg     5340

cctggacgtg aaattccaca cacaggcttt ttccgctcac ggcagcggga gagaagtcat     5400

tgatgccatg tgccatgcca ccctaactta caggatgttg gaaccaacta gggttgttaa     5460

ctgggaagtg atcattatgg atgaagccca ttttttggat ccagccagca tagccgctag     5520

aggttgggca gcgcacagag ctagggcaaa tgaaagtgca acaatcttga tgacagccac     5580

accgcctggg actagtgatg aatttccaca ttcaaatggt gaaatagaag atgttcaaac     5640

ggacataccc agtgagccct ggaacacagg gcatgactgg atcctggctg acaaaaggcc     5700

cacggcatgg ttccttccat ccatcagagc tgcaaatgtc atggctgcct ctttgcgtaa     5760

ggctggaaag agtgtggtgg tcctgaacag gaaaaccttt gagagagaat accccacgat     5820

aaagcagaag aaacctgact ttatattggc cactgacata gctgaaatgg gagccaacct     5880

ttgcgtggag cgagtgctgg attgcaggac ggcttttaag cctgtgcttg tggatgaagg     5940

gaggaaggtg gcaataaaag ggccacttcg tatctccgca tcctctgctg ctcaaaggag     6000

ggggcgcatt gggagaaatc ccaacagaga tggagactca tactactatt ctgagcctac     6060

aagtgaaaat aatgcccacc acgtctgctg gttggaggcc tcaatgctct tggacaacat     6120

ggaggtgagg ggtggaatgg tcgccccact ctatggcgtt gaaggaacta aaacaccagt     6180

ttcccctggt gaaatgagac tgagggatga ccagaggaaa gtcttcagag aactagtgag     6240

gaattgtgac ctgcccgttt ggctttcgtg gcaagtggcc aaggctggtt tgaagacgaa     6300

tgatcgtaag tggtgttttg aaggccctga ggaacatgag atcttgaatg acagcggtga     6360

aacagtgaag tgcagggctc ctggaggagc aaagaagcct ctgcgcccaa ggtggtgtga     6420

tgaaagggtg tcatctgacc agagtgcgct gtctgaattt attaagtttg ctgaaggtag     6480

gaggggagct gctgaagtgc tagttgtgct gagtgaactc cctgatttcc tggctaaaaa     6540

aggtggagag gcaatggata ccatcagtgt gttcctccac tctgaggaag gctctagggc     6600

ttaccgcaat gcactatcaa tgatgcctga ggcaatgaca atagtcatgc tgtttatact     6660

ggctggacta ctgacatcgg gaatggtcat ctttttcatg tctcccaaag gcatcagtag     6720

aatgtctatg gcgatgggca caatggccgg ctgtggatat ctcatgttcc ttggaggcgt     6780

caaacccact cacatctcct atgtcatgct catattcttt gtcctgatgg tggttgtgat     6840

ccccgagcca gggcaacaaa ggtccatcca agacaaccaa gtggcatacc tcattattgg     6900

catcctgacg ctggtttcag cggtggcagc caacgagcta ggtatgctgg agaaaaccaa     6960

agaggacctc tttgggaaga agaacttaat tccatctagt gcttcaccct ggagttggcc     7020

ggatcttgac ctgaagccag gagctgcctg gacagtgtac gttggcattg ttacaatgct     7080

ctctccaatg ttgcaccact ggatcaaagt cgaatatggc aacctgtctc tgtctggaat     7140

agcccagtca gcctcagtcc tttctttcat ggacaagggg ataccattca tgaagatgaa     7200

tatctcggtc ataatgctgc tggtcagtgg ctggaattca ataacagtga tgcctctgct     7260

ctgtggcata gggtgcgcca tgctccactg gtctctcatt ttacctggaa tcaaagcgca     7320

gcagtcaaag cttgcacaga gaagggtgtt ccatggcgtt gccaagaacc ctgtggttga     7380

tgggaatcca acagttgaca ttgaggaagc tcctgaaatg cctgcccttt atgagaagaa     7440

actggctcta tatctccttc ttgctctcag cctagcttct gttgccatgt gcagaacgcc     7500

cttttcattg gctgaaggca ttgtcctagc atcagctgcc ttagggccgc tcatagaggg     7560

aaacaccagc cttctttgga atggacccat ggctgtctcc atgacaggag tcatgagggg     7620

gaatcactat gcttttgtgg gagtcatgta caatctatgg aagatgaaaa ctggacgccg     7680

ggggagcgcg aatggaaaaa ctttgggtga agtctggaag agggaactga atctgttgga     7740

caagcgacag tttgagttgt ataaaaggac cgacattgtg gaggtggatc gtgatacggc     7800

acgcaggcat ttggccgaag ggaaggtgga caccggggtg gcggtctcca gggggaccgc     7860

aaagttaagg tggttccatg agcgtggcta tgtcaagctg gaaggtaggg tgattgacct     7920

ggggtgtggc cgcggaggct ggtgttacta cgctgctgcg caaaaggaag tgagtggggt     7980

caaaggattt actcttggaa gagacggcca tgagaaaccc atgaatgtgc aaagtctggg     8040

atggaacatc atcaccttca aggacaaaac tgatatccac cgcctagaac cagtgaaatg     8100

tgacaccctt ttgtgtgaca ttggagagtc atcatcgtca tcggtcacag agggggaaag     8160

gaccgtgaga gttcttgata ctgtagaaaa atggctggct tgtggggttg acaacttctg     8220

tgtgaaggtg ttagctccat acatgccaga tgttcttgag aaactggaat tgctccaaag     8280

gaggtttggc ggaacagtga tcaggaaccc tctctccagg aattccactc atgaaatgta     8340

ctacgtgtct ggagcccgca gcaatgtcac atttactgtg aaccaaacat cccgcctcct     8400

gatgaggaga atgaggcgtc caactggaaa agtgaccctg gaggctgacg tcatcctccc     8460

aattgggaca cgcagtgttg agacagacaa gggacccctg gacaaagagg ccatagaaga     8520

aagggttgag aggataaaat ctgagtacat gacctcttgg ttttatgaca atgacaaccc     8580

ctacaggacc tggcactact gtggctccta tgtcacaaaa acctccggaa gtgcggcgag     8640

catggtaaat ggtgttatta aaattctgac atatccatgg gacaggatag aggaggtcac     8700

aagaatggca atgactgaca caaccccttt tggacagcaa agagtgttta aagaaaaagt     8760

tgacaccaga gcaaaggatc caccagcggg aactaggaag atcatgaaag ttgtcaacag     8820

gtggctgttc cgccacctgg ccagagaaaa gaaccccaga ctgtgcacaa aggaagaatt     8880

tattgcaaaa gtccgaagtc atgcagccat tggagcttac ctggaagaac aagaacagtg     8940

gaagactgcc aatgaggctg tccaagaccc aaagttctgg gaactggtgg atgaagaaag     9000

gaagctgcac caacaaggca ggtgtcggac ttgtgtgtac aacatgatgg ggaaaagaga     9060

gaagaagctg tcagagtttg ggaaagcaaa gggaagccgt gccatatggt atatgtggct     9120

gggagcgcgg tatcttgagt ttgaggccct gggattcctg aatgaggacc attgggcttc     9180

cagggaaaac tcaggaggag gagtggaagg cattggctta caatacctag gatatgtgat     9240

cagagacctg gctgcaatgg atggtggtgg attctacgcg gatgacaccg ctggatggga     9300

cacgcgcatc acagaggcag accttgatga tgaacaggag atcttgaact acatgagccc     9360

acatcacaaa aaactggcac aagcagtgat ggaaatgaca tacaagaaca aagtggtgaa     9420

agtgttgaga ccagccccag gagggaaagc ctacatggat gtcataagtc gacgagacca     9480

gagaggatcc gggcaggtag tgacttatgc tctgaacacc atcaccaact tgaaagtcca     9540

attgatcaga atggcagaag cagagatggt gatacatcac caacatgttc aagattgtga     9600

tgaatcagtt ctgaccaggc tggaggcatg gctcactgag cacggatgtg acagactgaa     9660

gaggatggcg gtgagtggag acgactgtgt ggtccggccc atcgatgaca ggttcggcct     9720

ggccctgtcc catctcaacg ccatgtccaa ggttagaaag gacatatctg aatggcagcc     9780

atcaaaaggg tggaatgatt gggagaatgt gcccttctgt tcccaccact tccatgaact     9840

acagctgaag gatggcagga ggattgtggt gccttgccga gaacaggacg agctcattgg     9900

gagaggaagg gtgtctccag gaaacggctg gatgatcaag gaaacagctt gcctcagcaa     9960

agcctatgcc aacatgtggt cactgatgta ttttcacaaa agggacatga ggctactgtc    10020

attggctgtt tcctcagctg ttcccacctc atgggttcca caaggacgca caacatggtc    10080

gattcatggg aaaggggagt ggatgaccac ggaagacatg cttgaggtgt ggaacagagt    10140

atggataacc aacaacccac acatgcagga caagacaatg gtgaaaaaat ggagagatgt    10200

cccttatcta accaagagac aagacaagct gtgcggatca ctgattggaa tgaccaatag    10260

ggccacctgg gcctcccaca tccatttagt catccatcgt atccgaacgc tgattggaca    10320

ggagaaatac actgactacc taacagtcat ggacaggtat tctgtggatg ctgacctgca    10380

actgggtgag cttatctgaa acaccatcta acaggaataa ccgggataca aaccacgggt    10440

ggagaaccgg actccccaca acctgaaacc gggatataaa ccacggctgg agaaccggac    10500

tccgcactta aaatgaaaca gaaaccggga taaaaactac ggatggagaa ccggactcca    10560

cacattgaga cagaagaagt tgtcagccca gaaccccaca cgagttttgc cactgctaag    10620

ctgtgaggca gtgcaggctg ggacagccga cctccaggtt gcgaaaaacc tggtttctgg    10680

gacctcccac cccagagtaa aaagaacgga gcctccgcta ccaccctccc acgtggtggt    10740

agaaagacgg ggtctagagg ttagaggaga ccctccaggg aacaaatagt gggaccatat    10800

tgacgccagg gaaagaccgg agtggttctc tgcttttcct ccagaggtct gtgagcacag    10860

tttgctcaag aataagcaga cctttggatg acaaacacaa aaccact                  10907


<210>  11
<211>  14
<212>  DNA
<213>  artificial

<220>
<223>  primers

<400>  11
ycaycacttc aaca                                                         14


<210>  12
<211>  18
<212>  DNA
<213>  artificial

<220>
<223>  primers

<400>  12
ggaagargty ccgttytg                                                     18


<210>  13
<211>  18
<212>  DNA
<213>  artificial

<220>
<223>  primers

<400>  13
gccaatcagt tcatcttg                                                     18


