                         SEQUENCE LISTING

<110>  BAVARIAN NORDIC A/S
 
<120>  COMPOSITIONS AND METHODS FOR ENHANCING THE STABILITY OF 
       TRANSGENES IN POXVIRUSES

<130>  BNIT0011PCT

<140>  
<141>  

<160>  35    

<170>  PatentIn version 3.5

<210>  1
<211>  1548
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  MUC1 coding sequence from PANVAC

<400>  1
atgacaccgg gcacccagtc tcctttcttc ctgctgctgc tcctcacagt gcttacagtt       60

gttacgggtt ctggtcatgc aagctctacc ccaggtggag aaaaggagac ttcggctacc      120

cagagaagtt cagtgcccag ctctactgag aagaatgctg tgagtatgac aagctccgta      180

ctctccagcc acagccccgg ttcaggctcc tccaccactc agggacagga tgtcactctg      240

gccccggcca cggaaccagc ttcaggttca gctgccttgt ggggacagga tgtcacctcg      300

gtaccagtta ctagaccagc tttaggtagc acagcacctc ctgctcatgg agtaactagt      360

gctcctgata ctcgtccagc tcctggcagt actgcaccac cggcacatgg cgtaacatca      420

gcacctgata caagacctgc acctggatct acagcgccgc ctgcgcacgg agtgacatcg      480

gcgcccgata cgcgccccgc tcccggtagc accgcaccgc ccgcccacgg tgttacaagt      540

gcacccgata cccggccggc acccggaagt accgctccac ctgcacacgg ggtcacaagc      600

gcgccagaca ctcgacctgc gccagggtcg actgcccctc cggcgcatgg tgtgacctca      660

gctcctgaca caaggccagc cccagctagc actctggtgc acaacggcac ctctgccagg      720

gctaccacaa ccccagccag caagagcact ccattctcaa ttcccagcca ccactctgat      780

actcctacca cccttgccag ccatagcacc aagactgatg ccagtagcac tcaccatagc      840

acggtacctc ctctcacctc ctccaatcac agcacttctc cccagttgtc tactggggtc      900

tctttctttt tcctgtcttt tcacatttca aacctccagt ttaattcctc tctggaagat      960

cccagcaccg actactacca agagctgcag agagacattt ctgaaatgtt tttgcagatt     1020

tataaacaag ggggttttct gggcctctcc aatattaagt tcaggccagg atctgtggtg     1080

gtacaattga ctctggcctt ccgagaaggt accatcaatg tccacgacgt ggagacacag     1140

ttcaatcagt ataaaacgga agcagcctct cgatataacc tgacgatctc agacgtcagc     1200

gtgagtgatg tgccatttcc tttctctgcc cagtctgggg ctggggtgcc aggctggggc     1260

atcgcgctgc tggtgctggt ctgtgttctg gttgcgctgg ccattgtcta tctcattgcc     1320

ttggctgtct gtcagtgccg ccgaaagaac tacgggcagc tggacatctt tccagcccgg     1380

gatacctacc atcctatgag cgagtacccc acctaccaca cccatgggcg ctatgtgccc     1440

cctagcagta ccgatcgtag cccctatgag aaggtttctg caggtaatgg tggcagcagc     1500

ctctcttaca caaacccagc agtggcagcc acttctgcca acttgtag                  1548


<210>  2
<211>  1365
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  MUC1 coding sequence from mBN336

<400>  2
atgacaccgg gcacccagtc tcctttcttc ctgctgctgc tcctcacagt gcttacagtt       60

gttacgggtt ctggtcatgc aagctctacc ccaggtggag aaaaggagac ttcggctacc      120

cagagaagtt cagtgcccag ctctactgag aagaatgctg tgagtatgac aagctccgta      180

ctctccagcc acagcccagg ttcaggctcc tccaccactc agggacagga tgtcactctg      240

gcaccggcca cggaaccagc ttcaggttca gctgccttgt ggggacagga tgtcacctcg      300

gtaccagtta ctagaccagc tttaggctac ctggcgccac cggctcatgg cgttacatcg      360

tatttggaca ctcgaccggc accagttagc acagcacctc ccgcacacgg tgtaactagc      420

gcgcctgata cacgtcccgc tcccggatct accgctccgc cagcgcacgg agtgacgtca      480

gcaccagata cgaggccagc gcctgctagc actctggtgc acaacggcac ctctgccagg      540

gctaccacaa ccccagccag caagagcact ccattctcaa ttcccagcca ccactctgat      600

actcctacca cccttgccag ccatagcacc aagactgatg ccagtagcac tcaccatagc      660

acggtacctc ctctcacctc ctccaatcac agcacttctc cccagttgtc tactggggtc      720

tctttctttt tcctgtcttt tcacatttca aacctccagt ttaattcctc tctggaagat      780

cccagcaccg actactacca agagctgcag agagacattt ctgaaatgtt tttgcagatt      840

tataaacaag ggggttttct gggcctctcc aatattaagt tcaggccagg atctgtggtg      900

gtacagttga ctctggcctt ccgagaaggt accatcaatg tccacgacgt ggagacacag      960

ttcaatcagt ataaaacgga agcagcctct cgatataacc tgacgatctc agacgtcagc     1020

gtgagtgatg tgccatttcc tttctctgcc cagtctgggg ctggggtgcc aggctggggc     1080

atcgcgctgc tggtgctggt ctgtgttctg gtttacctgg ccattgtcta tctcattgcc     1140

ttggctgtct gtcaggtccg ccgaaagaac tacgggcagc tggacatctt tccagcccgg     1200

gatacctacc atcctatgag cgagtacccc acctaccaca cccatgggcg ctatgtgccc     1260

cctagcagtc tgttccgtag cccctatgag aaggtttctg caggtaatgg tggcagctac     1320

ctctcttaca caaacccagc agtggcagcc acttctgcca acttg                     1365


<210>  3
<211>  1365
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  MUC1 coding sequence from mBN373

<400>  3
atgacaccgg gcacccagtc tcctttcttc ctgctgctgc tcctcacagt gcttacagtt       60

gttacgggtt ctggtcatgc aagctctacc ccaggtggag aaaaggagac ttcggctacc      120

cagagaagtt cagtgcccag ctctactgag aagaatgctg tgagtatgac aagctccgta      180

ctctccagcc acagcccagg ttcaggctcc tccaccactc agggacagga tgtcactctg      240

gcaccggcca cggaaccagc ttcaggttca gctgccttgt ggggacagga tgtcacctcg      300

gtaccagtta ctagaccagc tttaggctac ctggcgccac cggctcatgg cgttacatcg      360

tatttggaca ctcgaccggc accagttagc acagcacctc ccgcacacgg tgtaactagc      420

gcgcctgata cacgtcccgc tcccggatct accgctccgc cagcgcacgg agtgacgtca      480

gcaccagata cgaggccagc gcctgctagc actctggtgc acaacggcac ctctgccagg      540

gctaccacaa ccccagccag caagagcact ccattctcaa ttcccagcca ccactctgat      600

actcctacca cccttgccag ccatagcacc aagactgatg ccagtagcac tcaccatagc      660

acggtacctc ctctcacctc ctccaatcac agcacttctc cccagttgtc tactggggtc      720

tctttctttt tcctgtcttt tcacatttca aacctccagt ttaattcctc tctggaagat      780

cccagcaccg actactacca agagctgcag agagacattt ctgaaatgtt tttgcagatt      840

tataaacaag ggggttttct gggcctctcc aatattaagt tcaggccagg atctgtggtg      900

gtacagttga ctctggcctt ccgagaaggt accatcaatg tccacgacgt ggagacacag      960

ttcaatcagt ataaaacgga agcagcctct cgatataacc tgacgatctc agacgtcagc     1020

gtgagtgatg tgccatttcc tttctctgcc cagtctgggg ctggggtgcc aggctggggc     1080

atcgcgctgc tggtgctggt ctgtgttctg gtttacctgg ccattgtcta tctcattgcc     1140

ttggctgtct gtcaggtccg ccgaaagaac tacgggcagc tggacatctt tccagcccgg     1200

gatacctacc atcctatgag cgagtacccc acctaccaca cccatgggcg ctatgtgccc     1260

cctagcagtc tgttccgtag cccctatgag aaggtttctg caggtaatgg tggcagctac     1320

ctctcttaca caaacccagc agtggcagcc acttctgcca acttg                     1365


<210>  4
<211>  1365
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  MUC1 coding sequence from mBN398 and mBN400

<400>  4
atgacacctg gcactcagtc accattcttc ctgctgttac tcttgacagt gcttacagtt       60

gttacaggtt ctggtcatgc aagctctacc ccaggtggag aaaaggagac ttcggctacc      120

cagcggagtt cagtgcctag ctctactgag aagaatgctg tgagtatgac aagctccgta      180

ctctccagcc acagcccagg ttcaggctcc agcaccactc aaggacagga tgtcactctg      240

gcaccggcca cggaaccagc ttcaggttca gctgccttgt ggggacagga tgtcacatcg      300

gtaccagtta ctagaccagc tttaggcagt actgcgccac cggctcatgg cgttacatcg      360

gcacctgaca ctcgaccggc accaggtagc acagcacctc ccgcacacgg tgtaactagc      420

gcgcctgata cacgtcccgc tcccggatct accgctccgc cagcgcacgg agtgacgtca      480

gcaccagata cgaggccagc gcctgctagc actctggtgc acaatggcac atctgccagg      540

gctaccacaa ctccagccag caagagcact ccattctcaa ttccaagcca tcactctgat      600

actcctacca cacttgccag ccatagcacc aagactgatg ccagtagcac tcaccatagc      660

acggtacctc cactcacctc atccaatcac agcacttctc ctcagttgtc tactggagtc      720

tccttctttt tcctgtcctt tcacatttca aacttgcagt tcaattcttc cctggaagat      780

cccagcaccg actactacca agagctgcag agagacattt ctgagatgtt cttgcagatt      840

tataaacaag gtggattcct tggcctctct aatattaagt tcaggccagg atctgtggtc      900

gtacagttga ctctggcctt cagagaaggt accatcaatg tccacgacgt ggagacacag      960

ttcaatcagt ataagacgga agcagcctca cgatataacc tgacgatctc agacgtcagc     1020

gttagtgatg tgccatttcc tttctctgcc cagtctggag ctggtgtgcc aggctggggc     1080

atcgcgctgc tcgtgttggt ctgtgttctg gttgcgctgg ccattgtcta tctcattgcc     1140

ttggctgttt gtcagtgcag acgcaagaac tacggacagc tggacatctt tccagctcgg     1200

gatacctacc atcctatgag cgagtaccct acctaccaca cacatggtcg ctatgtgcca     1260

cctagcagta ccgatcgtag tccctatgag aaagtttctg caggtaatgg tggcagcagc     1320

ctctcttaca caaacccagc agtggcagcc acttctgcca acttg                     1365


<210>  5
<211>  1365
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  MUC1 nucleic acid sequence from mBN420

<400>  5
atgacacctg gcactcagtc accattcttc ctgctgttac tcttgacagt gcttacagtt       60

gttacaggtt ctggtcatgc aagctctacc ccaggtggag aaaaggagac ttcggctacc      120

cagcggagtt cagtgcctag ctctactgag aagaatgctg tgagtatgac aagctccgta      180

ctctccagcc acagcccagg ttcaggctcc agcaccactc aaggacagga tgtcactctg      240

gcaccggcca cggaaccagc ttcaggttca gctgccttgt ggggacagga tgtcacatcg      300

gtaccagtta ctagaccagc tttaggctac ctggcgccac cggctcatgg cgttacatcg      360

tatttggaca ctcgaccggc accagttagc acagcacctc ccgcacacgg tgtaactagc      420

gcgcctgata cacgtcccgc tcccggatct accgctccgc cagcgcacgg agtgacgtca      480

gcaccagata cgaggccagc gcctgctagc actctggtgc acaatggcac atctgccagg      540

gctaccacaa ctccagccag caagagcact ccattctcaa ttccaagcca tcactctgat      600

actcctacca cacttgccag ccatagcacc aagactgatg ccagtagcac tcaccatagc      660

acggtacctc cactcacctc atccaatcac agcacttctc ctcagttgtc tactggagtc      720

tccttctttt tcctgtcctt tcacatttca aacttgcagt tcaattcttc cctggaagat      780

cccagcaccg actactacca agagctgcag agagacattt ctgagatgtt cttgcagatt      840

tataaacaag gtggattcct tggcctctct aatattaagt tcaggccagg atctgtggtc      900

gtacagttga ctctggcctt cagagaaggt accatcaatg tccacgacgt ggagacacag      960

ttcaatcagt ataagacgga agcagcctca cgatataacc tgacgatctc agacgtcagc     1020

gttagtgatg tgccatttcc tttctctgcc cagtctggag ctggtgtgcc aggctggggc     1080

atcgcgctgc tcgtgttggt ctgtgttctg gtttacctgg ccattgtcta tctcattgcc     1140

ttggctgttt gtcaggtcag acgcaagaac tacggacagc tggacatctt tccagctcgg     1200

gatacctacc atcctatgag cgagtaccct acctaccaca cacatggtcg ctatgtgcca     1260

cctagcagtc tgttccgtag tccctatgag aaagtttctg caggtaatgg tggcagctac     1320

ctctcttaca caaacccagc agtggcagcc acttctgcca acttg                     1365


<210>  6
<211>  515
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  MUC 1 amino acid sequence as found in PANVAC

<400>  6

Met Thr Pro Gly Thr Gln Ser Pro Phe Phe Leu Leu Leu Leu Leu Thr 
1               5                   10                  15      


Val Leu Thr Val Val Thr Gly Ser Gly His Ala Ser Ser Thr Pro Gly 
            20                  25                  30          


Gly Glu Lys Glu Thr Ser Ala Thr Gln Arg Ser Ser Val Pro Ser Ser 
        35                  40                  45              


Thr Glu Lys Asn Ala Val Ser Met Thr Ser Ser Val Leu Ser Ser His 
    50                  55                  60                  


Ser Pro Gly Ser Gly Ser Ser Thr Thr Gln Gly Gln Asp Val Thr Leu 
65                  70                  75                  80  


Ala Pro Ala Thr Glu Pro Ala Ser Gly Ser Ala Ala Leu Trp Gly Gln 
                85                  90                  95      


Asp Val Thr Ser Val Pro Val Thr Arg Pro Ala Leu Gly Ser Thr Ala 
            100                 105                 110         


Pro Pro Ala His Gly Val Thr Ser Ala Pro Asp Thr Arg Pro Ala Pro 
        115                 120                 125             


Gly Ser Thr Ala Pro Pro Ala His Gly Val Thr Ser Ala Pro Asp Thr 
    130                 135                 140                 


Arg Pro Ala Pro Gly Ser Thr Ala Pro Pro Ala His Gly Val Thr Ser 
145                 150                 155                 160 


Ala Pro Asp Thr Arg Pro Ala Pro Gly Ser Thr Ala Pro Pro Ala His 
                165                 170                 175     


Gly Val Thr Ser Ala Pro Asp Thr Arg Pro Ala Pro Gly Ser Thr Ala 
            180                 185                 190         


Pro Pro Ala His Gly Val Thr Ser Ala Pro Asp Thr Arg Pro Ala Pro 
        195                 200                 205             


Gly Ser Thr Ala Pro Pro Ala His Gly Val Thr Ser Ala Pro Asp Thr 
    210                 215                 220                 


Arg Pro Ala Pro Ala Ser Thr Leu Val His Asn Gly Thr Ser Ala Arg 
225                 230                 235                 240 


Ala Thr Thr Thr Pro Ala Ser Lys Ser Thr Pro Phe Ser Ile Pro Ser 
                245                 250                 255     


His His Ser Asp Thr Pro Thr Thr Leu Ala Ser His Ser Thr Lys Thr 
            260                 265                 270         


Asp Ala Ser Ser Thr His His Ser Thr Val Pro Pro Leu Thr Ser Ser 
        275                 280                 285             


Asn His Ser Thr Ser Pro Gln Leu Ser Thr Gly Val Ser Phe Phe Phe 
    290                 295                 300                 


Leu Ser Phe His Ile Ser Asn Leu Gln Phe Asn Ser Ser Leu Glu Asp 
305                 310                 315                 320 


Pro Ser Thr Asp Tyr Tyr Gln Glu Leu Gln Arg Asp Ile Ser Glu Met 
                325                 330                 335     


Phe Leu Gln Ile Tyr Lys Gln Gly Gly Phe Leu Gly Leu Ser Asn Ile 
            340                 345                 350         


Lys Phe Arg Pro Gly Ser Val Val Val Gln Leu Thr Leu Ala Phe Arg 
        355                 360                 365             


Glu Gly Thr Ile Asn Val His Asp Val Glu Thr Gln Phe Asn Gln Tyr 
    370                 375                 380                 


Lys Thr Glu Ala Ala Ser Arg Tyr Asn Leu Thr Ile Ser Asp Val Ser 
385                 390                 395                 400 


Val Ser Asp Val Pro Phe Pro Phe Ser Ala Gln Ser Gly Ala Gly Val 
                405                 410                 415     


Pro Gly Trp Gly Ile Ala Leu Leu Val Leu Val Cys Val Leu Val Ala 
            420                 425                 430         


Leu Ala Ile Val Tyr Leu Ile Ala Leu Ala Val Cys Gln Cys Arg Arg 
        435                 440                 445             


Lys Asn Tyr Gly Gln Leu Asp Ile Phe Pro Ala Arg Asp Thr Tyr His 
    450                 455                 460                 


Pro Met Ser Glu Tyr Pro Thr Tyr His Thr His Gly Arg Tyr Val Pro 
465                 470                 475                 480 


Pro Ser Ser Thr Asp Arg Ser Pro Tyr Glu Lys Val Ser Ala Gly Asn 
                485                 490                 495     


Gly Gly Ser Ser Leu Ser Tyr Thr Asn Pro Ala Val Ala Ala Thr Ser 
            500                 505                 510         


Ala Asn Leu 
        515 


<210>  7
<211>  60
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  VNTR #2 coding sequence from PANVAC

<400>  7
ggcagtactg caccaccggc acatggcgta acatcagcac ctgatacaag acctgcacct       60


<210>  8
<211>  60
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  VNTR #1 coding sequence from mBN420 and mBN336

<400>  8
ggctacctgg cgccaccggc tcatggcgtt acatcgtatt tggacactcg accggcacca       60


<210>  9
<211>  60
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  VNTR #1 coding sequence from PANVAC

<400>  9
ggtagcacag cacctcctgc tcatggagta actagtgctc ctgatactcg tccagctcct       60


<210>  10
<211>  60
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  VNTR #2 coding sequence from mBN420 and mBN336

<400>  10
gttagcacag cacctcccgc acacggtgta actagcgcgc ctgatacacg tcccgctccc       60


<210>  11
<211>  60
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  VNTR #3 coding sequence from PANVAC

<400>  11
ggatctacag cgccgcctgc gcacggagtg acatcggcgc ccgatacgcg ccccgctccc       60


<210>  12
<211>  60
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  VNTR #3 coding sequence from mBN420 and mBN336

<400>  12
ggatctaccg ctccgccagc gcacggagtg acgtcagcac cagatacgag gccagcgcct       60


<210>  13
<211>  2103
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  CEA coding sequence from PANVAC

<400>  13
atggagtctc cctcggcccc tccccacaga tggtgcatcc cctggcagag gctcctgctc       60

acagcctcac ttctaacctt ctggaacccg cccaccactg ccaagctcac tattgaatcc      120

acgccgttca atgtcgcaga ggggaaggag gtgcttctac ttgtccacaa tctgccccag      180

catctttttg gctacagctg gtacaaaggt gaaagagtgg atggcaaccg tcaaattata      240

ggatatgtaa taggaactca acaagctacc ccagggcccg catacagtgg tcgagagata      300

atatacccca atgcatccct gctgatccag aacatcatcc agaatgacac aggattctac      360

accctacacg tcataaagtc agatcttgtg aatgaagaag caactggcca gttccgggta      420

tacccggaac tccctaagcc ttctattagc tccaataata gtaagcctgt cgaagacaaa      480

gatgccgtcg cttttacatg cgagcccgaa actcaagacg caacatatct ctggtgggtg      540

aacaaccagt ccctgcctgt gtcccctaga ctccaactca gcaacggaaa tagaactctg      600

accctgttta acgtgaccag gaacgacaca gcaagctaca aatgcgaaac ccaaaatcca      660

gtcagcgcca ggaggtctga ttcagtgatt ctcaacgtgc tttacggacc cgatgctcct      720

acaatcagcc ctctaaacac aagctataga tcaggggaaa atctgaatct gagctgtcat      780

gccgctagca atcctcccgc ccaatacagc tggtttgtca atggcacttt ccaacagtcc      840

acccaggaac tgttcattcc caatattacc gtgaacaata gtggatccta cacgtgccaa      900

gctcacaata gcgacaccgg actcaaccgc acaaccgtga cgacgattac cgtgtatgag      960

ccaccaaaac cattcataac tagtaacaat tctaacccag ttgaggatga ggacgcagtt     1020

gcattaactt gtgagccaga gattcaaaat accacttatt tatggtgggt caataaccaa     1080

agtttgccgg ttagcccacg cttgcagttg tctaatgata accgcacatt gacactcctg     1140

tccgttactc gcaatgatgt aggaccttat gagtgtggca ttcagaatga attatccgtt     1200

gatcactccg accctgttat ccttaatgtt ttgtatggcc cagacgaccc aactatatct     1260

ccatcataca cctactaccg tcccggcgtg aacttgagcc tttcttgcca tgcagcatcc     1320

aacccccctg cacagtactc ctggctgatt gatggaaaca ttcagcagca tactcaagag     1380

ttatttataa gcaacataac tgagaagaac agcggactct atacttgcca ggccaataac     1440

tcagccagtg gtcacagcag gactacagtt aaaacaataa ctgtttccgc ggagctgccc     1500

aagccctcca tctccagcaa caactccaaa cccgtggagg acaaggatgc tgtggccttc     1560

acctgtgaac ctgaggctca gaacacaacc tacctgtggt gggtaaatgg tcagagcctc     1620

ccagtcagtc ccaggctgca gctgtccaat ggcaacagga ccctcactct attcaatgtc     1680

acaagaaatg acgcaagagc ctatgtatgt ggaatccaga actcagtgag tgcaaaccgc     1740

agtgacccag tcaccctgga tgtcctctat gggccggaca cccccatcat ttccccccca     1800

gactcgtctt acctttcggg agcggacctc aacctctcct gccactcggc ctctaaccca     1860

tccccgcagt attcttggcg tatcaatggg ataccgcagc aacacacaca agttctcttt     1920

atcgccaaaa tcacgccaaa taataacggg acctatgcct gttttgtctc taacttggct     1980

actggccgca ataattccat agtcaagagc atcacagtct ctgcatctgg aacttctcct     2040

ggtctctcag ctggggccac tgtcggcatc atgattggag tgctggttgg ggttgctctg     2100

ata                                                                   2103


<210>  14
<211>  2103
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  CEA coding sequence from mBN373 and mBN420

<400>  14
atggagtctc cctcggctcc tccacacaga tggtgcatcc cttggcagag gctcctgctc       60

acagcctcac ttctaacctt ctggaacccg cccaccactg ccaagctcac tattgaatcc      120

acgccgttca atgtcgcaga ggggaaggag gtgcttctac ttgtccacaa tctgcctcag      180

catctctttg gctacagctg gtacaaaggt gaaagagtgg atggcaaccg tcaaattata      240

ggatatgtaa taggaactca acaagctact ccagggcccg catacagtgg tcgagagata      300

atatacccta atgcatccct gctgatccag aacatcatcc agaatgacac aggattctac      360

accctacacg tcataaagtc agatcttgtg aatgaagaag caactggcca gttccgggta      420

taccctgaac tccctaagcc ttctattagc tccaataata gtaagcctgt cgaagacaaa      480

gatgccgtcg ctttcacatg cgagcccgaa actcaagacg caacatatct ctggtgggtg      540

aacaaccagt ccctgcctgt gtctcctaga ctccaactca gcaacggaaa tagaactctg      600

accctgttta acgtgaccag gaacgacaca gcaagctaca aatgcgaaac ccaaaatcca      660

gtcagcgcca ggaggtctga ttcagtgatt ctcaacgtgc tttacggacc cgatgctcct      720

acaatcagcc ctctaaacac aagctataga tcaggagaaa atctgaatct gagctgtcat      780

gccgctagca atcctccagc tcaatacagc tggtttgtca atggcacttt ccaacagtcc      840

acccaggaac tgttcattcc caatattacc gtgaacaata gtggatccta cacgtgccaa      900

gctcacaata gcgacaccgg actcaaccgc acaaccgtga cgacgattac cgtgtatgag      960

ccaccaaaac cattcataac tagtaacaat tctaacccag ttgaggatga ggacgcagtt     1020

gcattaactt gtgagccaga gattcaaaat accacttatt tatggtgggt caataaccaa     1080

agtttgccgg ttagcccacg cttgcagttg tctaatgata accgcacatt gacactcctg     1140

tccgttactc gcaatgatgt aggaccttat gagtgtggca ttcagaatga attatccgtt     1200

gatcactccg accctgttat ccttaatgtt ttgtatggcc cagacgaccc aactatatct     1260

ccatcataca cctactaccg tcccggcgtg aacttgagcc tttcttgcca tgcagcatct     1320

aatccacctg cacagtactc ctggctgatt gatggaaaca ttcagcagca tactcaagag     1380

ttatttataa gcaacataac tgagaagaac agcggactct atacttgcca ggccaataac     1440

tcagccagtg gtcacagcag gactacagtt aaaacaataa ctgtttccgc ggagctgccc     1500

aagccctcca tctccagcaa caactccaaa cccgtggagg acaaggatgc tgtggccttc     1560

acctgtgaac ctgaggctca gaacacaacc tacctgtggt gggtaaatgg tcagagcctc     1620

ccagtcagtc ccaggctgca gctgtccaat ggcaacagga ccctcactct attcaatgtc     1680

acaagaaatg acgcaagagc ctatgtatgt ggaatccaga actcagtgag tgcaaaccgc     1740

agtgacccag tcaccctgga tgtcctctat ggaccggaca cacccatcat ttcacctcca     1800

gactcgtctt acctttcggg agcggacctc aacctctcct gccactcggc ctctaaccca     1860

tctccgcagt attcttggcg tatcaatggg ataccgcagc aacacacaca agttctcttt     1920

atcgccaaaa tcacgccaaa taataacggg acctatgcct gttttgtctc taacttggct     1980

actggccgca ataattccat agtcaagagc atcacagtct ctgcatctgg aacttctcct     2040

ggtctctcag ctggagccac tgtcggcatc atgattggag tgctggttgg ggttgctctg     2100

ata                                                                   2103


<210>  15
<211>  864
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Optimized B7.1 coding sequence from mBN373 and mBN420

<400>  15
atgggacaca ccagaaggca gggcacaagc ccatccaagt gtccctacct gaacttcttt       60

cagctcctgg tgctggctgg cctgtcccac ttctgctccg gagtgatcca cgtgaccaag      120

gaggtcaaag aagtcgccac actgagctgc gggcacaatg tgtccgtgga ggaactggct      180

cagacacgga tctactggca gaaagagaag aaaatggtgc tgaccatgat gtccggcgac      240

atgaacatct ggcctgagta caagaaccgc accatcttcg acatcaccaa caatctgagc      300

atcgtgatcc tcgctctgag gccctccgac gagggaacat acgagtgcgt ggtgctgaag      360

tacgagaagg acgccttcaa acgcgagcac ctggccgagg tcaccctgtc cgtgaaggca      420

gacttcccaa cacccagcat cagcgacttc gagatcccta ccagcaacat ccggcggatt      480

atctgcagca cctccggagg cttcccagag cctcacctga gctggctcga gaacggcgaa      540

gagctcaacg ccatcaacac taccgtgtcc caggaccctg agacagagct gtacgctgtg      600

agcagcaagc tggacttcaa catgaccaca aatcacagct ttatgtgcct catcaagtac      660

ggccacctga gagtgaatca gaccttcaac tggaatacaa ccaagcagga acacttccca      720

gacaatctcc tgccctcctg ggctatcaca ctgattagcg tgaatggcat cttcgtgatc      780

tgctgtctga cctactgctt cgctcccaga tgccgggagc gcaggagaaa cgagaggctg      840

agacgggaat ccgtgaggcc cgtg                                             864


<210>  16
<211>  864
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  B7.1 coding sequence from PANVAC

<400>  16
atgggccaca cacggaggca gggaacatca ccatccaagt gtccatacct caatttcttt       60

cagctcttgg tgctggctgg tctttctcac ttctgttcag gtgttatcca cgtgaccaag      120

gaagtgaaag aagtggcaac gctgtcctgt ggtcacaatg tttctgttga agagctggca      180

caaactcgca tctactggca aaaggagaag aaaatggtgc tgactatgat gtctggagac      240

atgaatatat ggcccgagta caagaaccgg accatctttg atatcactaa taacctctcc      300

attgtgatcc tggctctgcg cccatctgac gagggcacat acgagtgtgt tgttctgaag      360

tatgaaaaag acgctttcaa gcgggaacac ctggctgaag tgacgttatc agtcaaagct      420

gacttcccta cacctagtat atctgacttt gaaattccaa cttctaatat tagaaggata      480

atttgctcaa cctctggagg ttttccagag cctcacctct cctggttgga aaatggagaa      540

gaattaaatg ccatcaacac aacagtttcc caagatcctg aaactgagct ctatgctgtt      600

agcagcaaac tggatttcaa tatgacaacc aaccacagct tcatgtgtct catcaagtat      660

ggacatttaa gagtgaatca gaccttcaac tggaatacaa ccaagcaaga gcattttcct      720

gataacctgc tcccatcctg ggccattacc ttaatctcag taaatggaat tttcgtgata      780

tgctgcctga cctactgctt tgccccacgc tgcagagaga gaaggaggaa tgagagattg      840

agaagggaaa gtgtacgccc tgta                                             864


<210>  17
<211>  864
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Optimized B7.1 coding sequence from mBN336

<400>  17
atgggccaca ccagaaggca gggcaccagc ccctccaagt gcccctacct gaacttcttc       60

cagctcctgg tgctggccgg cctgtcccac ttctgctccg gcgtgatcca cgtgaccaaa      120

gaggtcaaag aagtcgccac actgagctgc ggccacaatg tgtccgtgga ggaactggct      180

cagacccgga tctactggca gaaagaaaag aaaatggtgc tgaccatgat gtccggcgac      240

atgaacatct ggcctgagta caagaaccgc accatcttcg acatcaccaa caacctgagc      300

atcgtgatcc tcgccctgag gccctccgac gagggcacct acgagtgcgt ggtgctgaag      360

tacgagaagg acgccttcaa gcgcgagcac ctggccgagg tcaccctgtc cgtgaaggcc      420

gacttcccaa cccccagcat cagcgacttc gagatcccaa ccagcaacat ccggcggatc      480

atctgcagca cctccggcgg cttccccgag cctcacctga gctggctcga gaacggcgaa      540

gaactcaacg ccatcaacac taccgtgtcc caggaccccg agacagagct gtacgccgtg      600

agcagcaagc tggacttcaa catgaccaca aaccacagct ttatgtgcct catcaagtac      660

ggccacctga gagtgaatca gaccttcaac tggaacacca ccaagcagga acacttcccc      720

gacaatctgc tgccctcctg ggctatcacc ctgattagcg tgaatggcat cttcgtgatc      780

tgctgtctga cctactgctt cgcccccaga tgccgggagc ggcggagaaa cgagcggctg      840

cggcgggaat ccgtgaggcc cgtg                                             864


<210>  18
<211>  1596
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Optimized ICAM-1 coding sequence from mBN373 and mBN420

<400>  18
atggctccta gctcacctag accagctctg cctgccctgc tcgtgctgct cggagctctg       60

ttccctggac caggcaacgc ccagaccagc gtgtcaccta gcaaagtgat tctgcccaga      120

ggaggctccg tgctggtcac atgtagcacc agctgcgacc agcccaagct cctcgggatc      180

gagacacctc tgcccaagaa agagctgctc ctgccaggca acaatcggaa agtgtacgag      240

ctgtccaatg tgcaggaaga tagccagccc atgtgctact ccaactgtcc cgacggccag      300

agcaccgcca agacctttct gaccgtgtac tggacacctg agcgggtgga actggctcca      360

ctgcccagct ggcagccagt gggcaagaat ctgaccctgc ggtgccaggt ggaaggcgga      420

gctcccagag ccaacctgac agtggtgctc ctgagaggcg agaaagagct gaagcgggaa      480

cctgccgtgg gcgagccagc cgaagtgacc acaaccgtgc tcgtgcggag ggaccaccac      540

ggagccaact tcagctgcag aaccgagctg gacctcaggc cacagggcct ggaactgttc      600

gagaacacca gcgctcccta ccagctccag accttcgtgc tcccagcaac accacctcag      660

ctggtgtcac ctcgggtgct ggaagtggac acccagggca cagtcgtgtg cagcctggac      720

ggcctgtttc ccgtgtccga agctcaggtc cacctggctc tcggagacca gagactgaac      780

cctaccgtga cctacggcaa tgacagcttc agcgccaagg cctccgtgtc cgtgaccgcc      840

gaggatgaag gcacccagag gctgacatgc gccgtgattc tgggcaacca gagccaggaa      900

accctgcaga ccgtcaccat ctatagcttc cctgcaccta atgtgatcct gacaaagccc      960

gaggtgtccg agggcactga agtgaccgtg aaatgcgagg cccaccctag agccaaagtg     1020

accctgaacg gcgtgccagc ccagccactc ggaccaagag cacagctcct gctgaaagcc     1080

acacccgagg ataacggccg gtccttctcc tgcagcgcta ccctcgaagt ggccggacag     1140

ctgatccaca agaaccagac cagagagctg agagtgctgt acggccctag actggacgag     1200

agagactgcc caggcaactg gacctggccc gagaactccc agcagacacc catgtgccag     1260

gcttggggca acccactgcc agagctgaag tgcctgaagg acggcacctt ccctctgccc     1320

atcggcgagt ccgtgacagt gaccagggac ctggaaggca cctacctgtg cagagccaga     1380

tccacacagg gcgaagtgac acgggaggtc accgtgaatg tgctgtcacc tcgctacgag     1440

atcgtgatca tcaccgtggt cgctgcagct gtgatcatgg gcacagccgg actgagcaca     1500

tacctgtaca accggcagcg gaagatcaag aagtacaggc tgcagcaggc ccagaaaggc     1560

acacccatga agcccaacac ccaggccact cctccc                               1596


<210>  19
<211>  1596
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  ICAM-1 coding sequence from PANVAC

<400>  19
atggctccca gcagcccccg gcccgcgctg cccgcactcc tggtcctgct cggggctctg       60

ttcccaggac ctggcaatgc ccagacatct gtgtccccct caaaagtcat cctgccccgg      120

ggaggctccg tgctggtgac atgcagcacc tcctgtgacc agcccaagtt gttgggcata      180

gagaccccgt tgcctaaaaa ggagttgctc ctgcctggga acaaccggaa ggtgtatgaa      240

ctgagcaatg tgcaagaaga tagccaacca atgtgctatt caaactgccc tgatgggcag      300

tcaacagcta aaaccttcct caccgtgtac tggactccag aacgggtgga actggcaccc      360

ctcccctctt ggcagccagt gggcaagaac cttaccctac gctgccaggt ggagggtggg      420

gcaccccggg ccaacctcac cgtggtgctg ctccgtgggg agaaggagct gaaacgggag      480

ccagctgtgg gggagcccgc tgaggtcacg accacggtgc tggtgaggag agatcaccat      540

ggagccaatt tctcgtgccg cactgaactg gacctgcggc cccaagggct ggagctgttt      600

gagaacacct cggcccccta ccagctccag acctttgtcc tgccagcgac tcccccacaa      660

cttgtcagcc cccgggtcct agaggtggac acgcagggga ccgtggtctg ttccctggac      720

gggctgttcc cagtctcgga ggcccaggtc cacctggcac tgggggacca gaggttgaac      780

cccacagtca cctatggcaa cgactccttc tcggccaagg cctcagtcag tgtgaccgca      840

gaggacgagg gcacccagcg gctgacgtgt gcagtaatac tggggaacca gagccaggag      900

acactgcaga cagtgaccat ctacagcttt ccggcgccca acgtgattct gacgaagcca      960

gaggtctcag aagggaccga ggtgacagtg aagtgtgagg cccaccctag agccaaggtg     1020

acgctgaatg gggttccagc ccagccactg ggcccgaggg cccagctcct gctgaaggcc     1080

accccagagg acaacgggcg cagcttctcc tgctctgcaa ccctggaggt ggccggccag     1140

cttatacaca agaaccagac ccgggagctt cgtgtcctgt atggcccccg actggacgag     1200

agggattgtc cgggaaactg gacgtggcca gaaaattccc agcagactcc aatgtgccag     1260

gcttggggga acccattgcc cgagctcaag tgtctaaagg atggcacttt cccactgccc     1320

atcggggaat cagtgactgt cactcgagat cttgagggca cctacctctg tcgggccagg     1380

agcactcaag gggaggtcac ccgcgaggtg accgtgaatg tgctctcccc ccggtatgag     1440

attgtcatca tcactgtggt agcagccgca gtcataatgg gcactgcagg cctcagcacg     1500

tacctctata accgccagcg gaagatcaag aaatacagac tacaacaggc ccaaaaaggg     1560

acccccatga aaccgaacac acaagccacg cctccc                               1596


<210>  20
<211>  1596
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Optimized ICAM-1 coding sequence from mBN336

<400>  20
atggccccta gcagccctag accagccctg cctgccctgc tggtgctgct gggcgctctg       60

ttccccggac ccggcaacgc ccagaccagc gtgtccccca gcaaagtgat tctgcccaga      120

ggcggctccg tgctggtcac atgtagcacc agctgcgacc agcccaagct cctcgggatc      180

gagacacccc tgcccaagaa agagctgctg ctgcccggca acaaccggaa agtgtacgag      240

ctgtccaatg tgcaggaaga tagccagccc atgtgctact ccaactgccc cgacggccag      300

agcaccgcca agacctttct gaccgtgtac tggacccccg agcgggtgga actggcccca      360

ctgcccagct ggcagcccgt gggcaagaat ctgaccctgc ggtgccaggt ggaaggcgga      420

gcccccagag ccaacctgac agtggtgctc ctgcggggcg aaaaagagct gaagcgggag      480

cctgccgtgg gcgagccagc cgaagtgacc acaaccgtgc tcgtgcggag ggaccaccac      540

ggcgccaact tcagctgcag aaccgagctg gacctcaggc cacagggcct ggaactgttc      600

gagaacacca gcgcccccta ccagctccag accttcgtgc tcccagcaac cccccctcag      660

ctggtgtccc ctcgggtgct ggaagtggac acccagggca cagtcgtgtg cagcctggac      720

ggcctgtttc ccgtgtccga agctcaggtc cacctggctc tcggggacca gagactgaac      780

cctaccgtga cctacggcaa tgacagcttc agcgccaagg cctccgtgtc cgtgaccgcc      840

gaggatgagg gcacccagag gctgacatgc gccgtgattc tgggcaacca gagccaggaa      900

accctgcaga ccgtcaccat ctatagcttc cctgccccca atgtgatcct gacaaagccc      960

gaggtgtccg agggcactga agtgaccgtg aaatgcgagg cccaccccag ggccaaagtg     1020

accctgaacg gcgtgccagc ccagccactc ggaccaagag cacagctcct gctgaaagcc     1080

acccccgagg ataacggccg gtccttctcc tgcagcgcta ccctcgaagt ggccgggcag     1140

ctgatccaca agaaccagac ccgggagctg agagtgctgt acggccccag actggacgag     1200

agagactgcc ccggcaactg gacctggccc gagaactccc agcagacccc catgtgccag     1260

gcttggggca acccactgcc agagctgaag tgcctgaagg acggcacctt ccctctgccc     1320

atcggcgagt ccgtgacagt gacccgggac ctggaaggca cctacctgtg ccgggccaga     1380

tccacacagg gcgaagtgac acgggaggtc accgtgaatg tgctgtcccc ccgctacgag     1440

atcgtgatca tcaccgtggt cgctgcagct gtgatcatgg gcacagccgg cctgagcaca     1500

tacctgtaca accggcagcg gaagatcaag aagtacaggc tgcagcaggc ccagaaaggc     1560

acccccatga agcccaacac ccaggccacc cctccc                               1596


<210>  21
<211>  750
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Optimized LFA-3 coding sequence from mBN373 and mBN420

<400>  21
atggtggctg gctctgatgc agggagagcc ctgggagtgc tgtctgtcgt gtgcctgctg       60

cactgcttcg gcttcatcag ctgcttcagc cagcagatct acggagtggt ctacggcaac      120

gtgaccttcc acgtgcccag caacgtgcct ctgaaagagg tgctctggaa gaaacagaag      180

gacaaggtcg cagagctgga gaacagcgag ttccgggcct tcagcagctt caagaaccgg      240

gtgtacctgg acaccgtgtc cggcagcctg accatctaca acctgaccag cagcgacgag      300

gacgagtacg agatggaaag ccctaacatc accgacacca tgaagttctt tctgtacgtg      360

ctggaaagcc tgcccagccc aacactgacc tgtgccctga ccaacggctc catcgaggtg      420

cagtgcatga ttcccgagca ctacaactcc cacagaggcc tgatcatgta ctcttgggac      480

tgccctatgg aacagtgcaa gcgcaacagc accagcatct acttcaagat ggagaacgac      540

ctccctcaga agatccagtg cacactgagc aatccactgt tcaacaccac atccagcatc      600

atcctgacaa cctgtattcc cagcagtggc cacagcagac acagatacgc cctgatccct      660

attccactgg ccgtgatcac cacatgcatc gtgctgtaca tgaacggcat cctgaagtgc      720

gaccggaagc ccgaccggac caacagcaac                                       750


<210>  22
<211>  750
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  LFA-3 coding sequence from PANVAC

<400>  22
atggttgctg ggagcgacgc ggggcgggcc ctgggggtcc tcagcgtggt ctgcctgctg       60

cactgctttg gtttcatcag ctgtttttcc caacaaatat atggtgttgt gtatgggaat      120

gtaactttcc atgtaccaag caatgtgcct ttaaaagagg tcctatggaa aaaacaaaag      180

gataaagttg cagaactgga aaattctgaa ttcagagctt tctcatcttt taaaaatagg      240

gtttatttag acactgtgtc aggtagcctc actatctaca acttaacatc atcagatgaa      300

gatgagtatg aaatggaatc gccaaatatt actgatacca tgaagttctt tctttatgtg      360

cttgagtctc ttccatctcc cacactaact tgtgcattga ctaatggaag cattgaagtc      420

caatgcatga taccagagca ttacaacagc catcgaggac ttataatgta ctcatgggat      480

tgtcctatgg agcaatgtaa acgtaactca accagtatat attttaagat ggaaaatgat      540

cttccacaaa aaatacagtg tactcttagc aatccattat ttaatacaac atcatcaatc      600

attttgacaa cctgtatccc aagcagcggt cattcaagac acagatatgc acttataccc      660

ataccattag cagtaattac aacatgtatt gtgctgtata tgaatggtat tctgaaatgt      720

gacagaaaac cagacagaac caactccaat                                       750


<210>  23
<211>  750
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Optimized LFA-3 coding sequence from mBN336

<400>  23
atggtggctg gctctgatgc aggcagagcc ctgggcgtgc tgtctgtcgt gtgcctgctg       60

cactgcttcg gcttcatcag ctgcttcagc cagcagatct acggcgtggt gtacggcaac      120

gtgaccttcc acgtgcccag caacgtgcct ctgaaagagg tgctctggaa gaagcagaag      180

gacaaggtcg cagagctgga aaacagcgag ttccgggcct tcagcagctt caagaaccgg      240

gtgtacctgg acaccgtgtc cggcagcctg accatctaca acctgaccag cagcgacgag      300

gacgagtacg agatggaaag ccccaacatc accgacacca tgaagttctt tctgtacgtg      360

ctggaaagcc tgcccagccc caccctgacc tgtgccctga ccaacggctc catcgaggtg      420

cagtgcatga tccccgagca ctacaactcc caccggggcc tgatcatgta ctcttgggac      480

tgccctatgg aaacgtgcaa gcgcaacagc accagcatct acttcaagat ggaaaacgac      540

ctcccccaga aaatccagtg caccctgagc aaccccctgt tcaacaccac ctccagcatc      600

atcctgacca cctgtatccc cagcagcggc cacagcagac acagatacgc cctgatcccc      660

atccccctgg ccgtgatcac cacatgcatc gtgctgtaca tgaacggcat cctgaagtgc      720

gaccggaagc ccgaccggac caacagcaac                                       750


<210>  24
<211>  9
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  MUC1 Agonist Epitope

<400>  24

Tyr Leu Ala Pro Pro Ala His Gly Val 
1               5                   


<210>  25
<211>  9
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  MUC1 agonist epitope

<400>  25

Tyr Leu Asp Thr Arg Pro Ala Pro Val 
1               5                   


<210>  26
<211>  10
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  MUC1 agonist epitope

<400>  26

Tyr Leu Ala Ile Val Tyr Leu Ile Ala Leu 
1               5                   10  


<210>  27
<211>  10
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  MUC1 agonist epitope

<400>  27

Tyr Leu Ile Ala Leu Ala Val Cys Gln Val 
1               5                   10  


<210>  28
<211>  9
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  MUC1 agonist epitope

<400>  28

Tyr Leu Ser Tyr Thr Asn Pro Ala Val 
1               5                   


<210>  29
<211>  9
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  MUC1 agonist epitope

<400>  29

Ser Leu Phe Arg Ser Pro Tyr Glu Lys 
1               5                   


<210>  30
<211>  455
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  MUC1 amino acid sequence as found in mBN336, mBN373, and mBN420

<400>  30

Met Thr Pro Gly Thr Gln Ser Pro Phe Phe Leu Leu Leu Leu Leu Thr 
1               5                   10                  15      


Val Leu Thr Val Val Thr Gly Ser Gly His Ala Ser Ser Thr Pro Gly 
            20                  25                  30          


Gly Glu Lys Glu Thr Ser Ala Thr Gln Arg Ser Ser Val Pro Ser Ser 
        35                  40                  45              


Thr Glu Lys Asn Ala Val Ser Met Thr Ser Ser Val Leu Ser Ser His 
    50                  55                  60                  


Ser Pro Gly Ser Gly Ser Ser Thr Thr Gln Gly Gln Asp Val Thr Leu 
65                  70                  75                  80  


Ala Pro Ala Thr Glu Pro Ala Ser Gly Ser Ala Ala Leu Trp Gly Gln 
                85                  90                  95      


Asp Val Thr Ser Val Pro Val Thr Arg Pro Ala Leu Gly Tyr Leu Ala 
            100                 105                 110         


Pro Pro Ala His Gly Val Thr Ser Tyr Leu Asp Thr Arg Pro Ala Pro 
        115                 120                 125             


Val Ser Thr Ala Pro Pro Ala His Gly Val Thr Ser Ala Pro Asp Thr 
    130                 135                 140                 


Arg Pro Ala Pro Gly Ser Thr Ala Pro Pro Ala His Gly Val Thr Ser 
145                 150                 155                 160 


Ala Pro Asp Thr Arg Pro Ala Pro Ala Ser Thr Leu Val His Asn Gly 
                165                 170                 175     


Thr Ser Ala Arg Ala Thr Thr Thr Pro Ala Ser Lys Ser Thr Pro Phe 
            180                 185                 190         


Ser Ile Pro Ser His His Ser Asp Thr Pro Thr Thr Leu Ala Ser His 
        195                 200                 205             


Ser Thr Lys Thr Asp Ala Ser Ser Thr His His Ser Thr Val Pro Pro 
    210                 215                 220                 


Leu Thr Ser Ser Asn His Ser Thr Ser Pro Gln Leu Ser Thr Gly Val 
225                 230                 235                 240 


Ser Phe Phe Phe Leu Ser Phe His Ile Ser Asn Leu Gln Phe Asn Ser 
                245                 250                 255     


Ser Leu Glu Asp Pro Ser Thr Asp Tyr Tyr Gln Glu Leu Gln Arg Asp 
            260                 265                 270         


Ile Ser Glu Met Phe Leu Gln Ile Tyr Lys Gln Gly Gly Phe Leu Gly 
        275                 280                 285             


Leu Ser Asn Ile Lys Phe Arg Pro Gly Ser Val Val Val Gln Leu Thr 
    290                 295                 300                 


Leu Ala Phe Arg Glu Gly Thr Ile Asn Val His Asp Val Glu Thr Gln 
305                 310                 315                 320 


Phe Asn Gln Tyr Lys Thr Glu Ala Ala Ser Arg Tyr Asn Leu Thr Ile 
                325                 330                 335     


Ser Asp Val Ser Val Ser Asp Val Pro Phe Pro Phe Ser Ala Gln Ser 
            340                 345                 350         


Gly Ala Gly Val Pro Gly Trp Gly Ile Ala Leu Leu Val Leu Val Cys 
        355                 360                 365             


Val Leu Val Tyr Leu Ala Ile Val Tyr Leu Ile Ala Leu Ala Val Cys 
    370                 375                 380                 


Gln Val Arg Arg Lys Asn Tyr Gly Gln Leu Asp Ile Phe Pro Ala Arg 
385                 390                 395                 400 


Asp Thr Tyr His Pro Met Ser Glu Tyr Pro Thr Tyr His Thr His Gly 
                405                 410                 415     


Arg Tyr Val Pro Pro Ser Ser Leu Phe Arg Ser Pro Tyr Glu Lys Val 
            420                 425                 430         


Ser Ala Gly Asn Gly Gly Ser Tyr Leu Ser Tyr Thr Asn Pro Ala Val 
        435                 440                 445             


Ala Ala Thr Ser Ala Asn Leu 
    450                 455 


<210>  31
<211>  1365
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  MUC1 coding sequence for mBN336 w/o agonist epitopes from WO 
       2013/103658

<400>  31
atgacaccgg gcacccagtc tcctttcttc ctgctgctgc tcctcacagt gcttacagtt       60

gttacgggtt ctggtcatgc aagctctacc ccaggtggag aaaaggagac ttcggctacc      120

cagagaagtt cagtgcccag ctctactgag aagaatgctg tgagtatgac aagctccgta      180

ctctccagcc acagcccagg ttcaggctcc tccaccactc agggacagga tgtcactctg      240

gcaccggcca cggaaccagc ttcaggttca gctgccttgt ggggacagga tgtcacctcg      300

gtaccagtta ctagaccagc tttaggcagc acagcgccac cggctcatgg cgttacatcg      360

gctcctgaca ctcgaccggc accaggcagc acagcacctc ccgcacacgg tgtaactagc      420

gcgcctgata cacgtcccgc tcccggatct accgctccgc cagcgcacgg agtgacgtca      480

gcaccagata cgaggccagc gcctgctagc actctggtgc acaacggcac ctctgccagg      540

gctaccacaa ccccagccag caagagcact ccattctcaa ttcccagcca ccactctgat      600

actcctacca cccttgccag ccatagcacc aagactgatg ccagtagcac tcaccatagc      660

acggtacctc ctctcacctc ctccaatcac agcacttctc cccagttgtc tactggggtc      720

tctttctttt tcctgtcttt tcacatttca aacctccagt ttaattcctc tctggaagat      780

cccagcaccg actactacca agagctgcag agagacattt ctgaaatgtt tttgcagatt      840

tataaacaag ggggttttct gggcctctcc aatattaagt tcaggccagg atctgtggtg      900

gtacagttga ctctggcctt ccgagaaggt accatcaatg tccacgacgt ggagacacag      960

ttcaatcagt ataaaacgga agcagcctct cgatataacc tgacgatctc agacgtcagc     1020

gtgagtgatg tgccatttcc tttctctgcc cagtctgggg ctggggtgcc aggctggggc     1080

atcgcgctgc tggtgctggt ctgtgttctg gttgcactgg ccattgtcta tctcattgcc     1140

ttggctgtct gtcagtgccg ccgaaagaac tacgggcagc tggacatctt tccagcccgg     1200

gatacctacc atcctatgag cgagtacccc acctaccaca cccatgggcg ctatgtgccc     1260

cctagcagta ccgatcgtag cccctatgag aaggtttctg caggtaatgg tggcagcagt     1320

ctctcttaca caaacccagc agtggcagcc acttctgcca acttg                     1365


<210>  32
<211>  1365
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  MUC1 coding sequence for mBN373 w/o agonist epitopes from WO 
       2013/103658

<400>  32
atgacaccgg gcacccagtc tcctttcttc ctgctgctgc tcctcacagt gcttacagtt       60

gttacgggtt ctggtcatgc aagctctacc ccaggtggag aaaaggagac ttcggctacc      120

cagagaagtt cagtgcccag ctctactgag aagaatgctg tgagtatgac aagctccgta      180

ctctccagcc acagcccagg ttcaggctcc tccaccactc agggacagga tgtcactctg      240

gcaccggcca cggaaccagc ttcaggttca gctgccttgt ggggacagga tgtcacctcg      300

gtaccagtta ctagaccagc tttaggcagc acagcgccac cggctcatgg cgttacatcg      360

gctcctgaca ctcgaccggc accaggcagc acagcacctc ccgcacacgg tgtaactagc      420

gcgcctgata cacgtcccgc tcccggatct accgctccgc cagcgcacgg agtgacgtca      480

gcaccagata cgaggccagc gcctgctagc actctggtgc acaacggcac ctctgccagg      540

gctaccacaa ccccagccag caagagcact ccattctcaa ttcccagcca ccactctgat      600

actcctacca cccttgccag ccatagcacc aagactgatg ccagtagcac tcaccatagc      660

acggtacctc ctctcacctc ctccaatcac agcacttctc cccagttgtc tactggggtc      720

tctttctttt tcctgtcttt tcacatttca aacctccagt ttaattcctc tctggaagat      780

cccagcaccg actactacca agagctgcag agagacattt ctgaaatgtt tttgcagatt      840

tataaacaag ggggttttct gggcctctcc aatattaagt tcaggccagg atctgtggtg      900

gtacagttga ctctggcctt ccgagaaggt accatcaatg tccacgacgt ggagacacag      960

ttcaatcagt ataaaacgga agcagcctct cgatataacc tgacgatctc agacgtcagc     1020

gtgagtgatg tgccatttcc tttctctgcc cagtctgggg ctggggtgcc aggctggggc     1080

atcgcgctgc tggtgctggt ctgtgttctg gttgcactgg ccattgtcta tctcattgcc     1140

ttggctgtct gtcagtgccg ccgaaagaac tacgggcagc tggacatctt tccagcccgg     1200

gatacctacc atcctatgag cgagtacccc acctaccaca cccatgggcg ctatgtgccc     1260

cctagcagta ccgatcgtag cccctatgag aaggtttctg caggtaatgg tggcagcagt     1320

ctctcttaca caaacccagc agtggcagcc acttctgcca acttg                     1365


<210>  33
<211>  1365
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  MUC1 coding sequence for mBN420 w/o agonist epitopes from WO 
       2013/103658

<400>  33
atgacacctg gcactcagtc accattcttc ctgctgttac tcttgacagt gcttacagtt       60

gttacaggtt ctggtcatgc aagctctacc ccaggtggag aaaaggagac ttcggctacc      120

cagcggagtt cagtgcctag ctctactgag aagaatgctg tgagtatgac aagctccgta      180

ctctccagcc acagcccagg ttcaggctcc agcaccactc aaggacagga tgtcactctg      240

gcaccggcca cggaaccagc ttcaggttca gctgccttgt ggggacagga tgtcacatcg      300

gtaccagtta ctagaccagc tttaggcagc acagcgccac cggctcatgg cgttacatcg      360

gctcctgaca ctcgaccggc accaggcagc acagcacctc ccgcacacgg tgtaactagc      420

gcgcctgata cacgtcccgc tcccggatct accgctccgc cagcgcacgg agtgacgtca      480

gcaccagata cgaggccagc gcctgctagc actctggtgc acaatggcac atctgccagg      540

gctaccacaa ctccagccag caagagcact ccattctcaa ttccaagcca tcactctgat      600

actcctacca cacttgccag ccatagcacc aagactgatg ccagtagcac tcaccatagc      660

acggtacctc cactcacctc atccaatcac agcacttctc ctcagttgtc tactggagtc      720

tccttctttt tcctgtcctt tcacatttca aacttgcagt tcaattcttc cctggaagat      780

cccagcaccg actactacca agagctgcag agagacattt ctgagatgtt cttgcagatt      840

tataaacaag gtggattcct tggcctctct aatattaagt tcaggccagg atctgtggtc      900

gtacagttga ctctggcctt cagagaaggt accatcaatg tccacgacgt ggagacacag      960

ttcaatcagt ataagacgga agcagcctca cgatataacc tgacgatctc agacgtcagc     1020

gttagtgatg tgccatttcc tttctctgcc cagtctggag ctggtgtgcc aggctggggc     1080

atcgcgctgc tcgtgttggt ctgtgttctg gttgcactgg ccattgtcta tctcattgcc     1140

ttggctgttt gtcagtgcag acgcaagaac tacggacagc tggacatctt tccagctcgg     1200

gatacctacc atcctatgag cgagtaccct acctaccaca cacatggtcg ctatgtgcca     1260

cctagcagta ccgatcgtag tccctatgag aaagtttctg caggtaatgg tggcagcagt     1320

ctctcttaca caaacccagc agtggcagcc acttctgcca acttg                     1365


<210>  34
<211>  1365
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  MUC1 coding sequence for mBN optimized w/o agonist epitopes from 
       WO 2013/103658

<400>  34
atgacacctg gcactcagtc accattcttc ctgctgttac tcttgacagt gcttacagtt       60

gttacaggtt ctggtcatgc aagctctacc ccaggtggag aaaaggagac ttcggctacc      120

cagcggagtt cagtgcctag ctctactgag aagaatgctg tgagtatgac aagctccgta      180

ctctccagcc acagcccagg ttcaggctcc agcaccactc aaggacagga cgtcactctg      240

gcaccggcca cggaaccagc ttcaggttca gctgccttgt ggggacagga tgtgacatcg      300

gtaccagtta ctagaccagc tttaggcagc acagcgccac cggctcatgg cgttacatcg      360

gctcctgaca ctcgaccggc accaggcagc acagcacctc ccgcacacgg tgtaactagc      420

gcgcctgata cacgtcccgc tcccggatct accgctccgc cagcgcacgg agtgacgtca      480

gcaccagata cgaggccagc gcctgctagc actctggtgc acaatggcac atctgccagg      540

gctaccacaa ctccagccag caagagcact ccattctcaa ttccaagcca tcactctgat      600

actcctacca cacttgccag ccatagcacc aagactgatg ccagtagcac tcaccatagc      660

acggtacctc cactcacctc atccaatcac agcacttctc ctcagttgtc tactggagtc      720

tccttctttt tcctgtcctt tcacatttca aacttgcagt tcaattcttc cctggaagat      780

cccagcaccg actactacca agagctgcag agagacattt ctgagatgtt cttgcagatt      840

tataaacaag gtggattcct tggcctctct aatattaagt tcaggccagg atctgtggtc      900

gtacagttga ctctggcctt cagagaaggt accatcaatg tccacgacgt ggagacacag      960

ttcaatcagt ataagacgga agcagcctca cgatataacc tgacgatctc agacgtcagc     1020

gttagtgatg tgccatttcc tttctctgcc cagtctggag ctggtgtgcc aggctggggc     1080

atcgcgctgc tcgtgttggt ctgtgttctg gttgcactgg ccattgtcta tctcattgcc     1140

ttggctgttt gtcagtgcag acgcaagaac tacggacagc tggacatctt tccagctcgg     1200

gatacctacc atcctatgag cgagtaccct acctaccaca cacatggtcg ctatgtgcca     1260

cctagcagta ccgatcgtag tccctatgag aaagtttctg caggtaatgg tggcagcagt     1320

ctctcttaca caaacccagc agtggcagcc acttctgcca acttg                     1365


<210>  35
<211>  455
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  MUC1 amino acid from mBN336/mBN373/mBN420 w/o agonist epitopes 
       from WO 2013/103658

<400>  35

Met Thr Pro Gly Thr Gln Ser Pro Phe Phe Leu Leu Leu Leu Leu Thr 
1               5                   10                  15      


Val Leu Thr Val Val Thr Gly Ser Gly His Ala Ser Ser Thr Pro Gly 
            20                  25                  30          


Gly Glu Lys Glu Thr Ser Ala Thr Gln Arg Ser Ser Val Pro Ser Ser 
        35                  40                  45              


Thr Glu Lys Asn Ala Val Ser Met Thr Ser Ser Val Leu Ser Ser His 
    50                  55                  60                  


Ser Pro Gly Ser Gly Ser Ser Thr Thr Gln Gly Gln Asp Val Thr Leu 
65                  70                  75                  80  


Ala Pro Ala Thr Glu Pro Ala Ser Gly Ser Ala Ala Leu Trp Gly Gln 
                85                  90                  95      


Asp Val Thr Ser Val Pro Val Thr Arg Pro Ala Leu Gly Ser Thr Ala 
            100                 105                 110         


Pro Pro Ala His Gly Val Thr Ser Ala Pro Asp Thr Arg Pro Ala Pro 
        115                 120                 125             


Gly Ser Thr Ala Pro Pro Ala His Gly Val Thr Ser Ala Pro Asp Thr 
    130                 135                 140                 


Arg Pro Ala Pro Gly Ser Thr Ala Pro Pro Ala His Gly Val Thr Ser 
145                 150                 155                 160 


Ala Pro Asp Thr Arg Pro Ala Pro Ala Ser Thr Leu Val His Asn Gly 
                165                 170                 175     


Thr Ser Ala Arg Ala Thr Thr Thr Pro Ala Ser Lys Ser Thr Pro Phe 
            180                 185                 190         


Ser Ile Pro Ser His His Ser Asp Thr Pro Thr Thr Leu Ala Ser His 
        195                 200                 205             


Ser Thr Lys Thr Asp Ala Ser Ser Thr His His Ser Thr Val Pro Pro 
    210                 215                 220                 


Leu Thr Ser Ser Asn His Ser Thr Ser Pro Gln Leu Ser Thr Gly Val 
225                 230                 235                 240 


Ser Phe Phe Phe Leu Ser Phe His Ile Ser Asn Leu Gln Phe Asn Ser 
                245                 250                 255     


Ser Leu Glu Asp Pro Ser Thr Asp Tyr Tyr Gln Glu Leu Gln Arg Asp 
            260                 265                 270         


Ile Ser Glu Met Phe Leu Gln Ile Tyr Lys Gln Gly Gly Phe Leu Gly 
        275                 280                 285             


Leu Ser Asn Ile Lys Phe Arg Pro Gly Ser Val Val Val Gln Leu Thr 
    290                 295                 300                 


Leu Ala Phe Arg Glu Gly Thr Ile Asn Val His Asp Val Glu Thr Gln 
305                 310                 315                 320 


Phe Asn Gln Tyr Lys Thr Glu Ala Ala Ser Arg Tyr Asn Leu Thr Ile 
                325                 330                 335     


Ser Asp Val Ser Val Ser Asp Val Pro Phe Pro Phe Ser Ala Gln Ser 
            340                 345                 350         


Gly Ala Gly Val Pro Gly Trp Gly Ile Ala Leu Leu Val Leu Val Cys 
        355                 360                 365             


Val Leu Val Ala Leu Ala Ile Val Tyr Leu Ile Ala Leu Ala Val Cys 
    370                 375                 380                 


Gln Cys Arg Arg Lys Asn Tyr Gly Gln Leu Asp Ile Phe Pro Ala Arg 
385                 390                 395                 400 


Asp Thr Tyr His Pro Met Ser Glu Tyr Pro Thr Tyr His Thr His Gly 
                405                 410                 415     


Arg Tyr Val Pro Pro Ser Ser Thr Asp Arg Ser Pro Tyr Glu Lys Val 
            420                 425                 430         


Ser Ala Gly Asn Gly Gly Ser Ser Leu Ser Tyr Thr Asn Pro Ala Val 
        435                 440                 445             


Ala Ala Thr Ser Ala Asn Leu 
    450                 455 


