                         SEQUENCE LISTING

<110>  Nanyang Technological University
 
<120>  METHODS FOR ENZYMATIC PEPTIDE LIGATION

<130>  P112356

<150>  Singapore Patent Application No. 10201607951V
<151>  2016-09-23

<160>  17    

<170>  PatentIn version 3.5

<210>  1
<211>  474
<212>  PRT
<213>  Ovaloparmena affinis

<400>  1

Met Val Arg Tyr Leu Ala Gly Ala Val Leu Leu Leu Val Val Leu Ser 
1               5                   10                  15      


Val Ala Ala Ala Val Ser Gly Ala Arg Asp Gly Asp Tyr Leu His Leu 
            20                  25                  30          


Pro Ser Glu Val Ser Arg Phe Phe Arg Pro Gln Glu Thr Asn Asp Asp 
        35                  40                  45              


His Gly Glu Asp Ser Val Gly Thr Arg Trp Ala Val Leu Ile Ala Gly 
    50                  55                  60                  


Ser Lys Gly Tyr Ala Asn Tyr Arg His Gln Ala Gly Val Cys His Ala 
65                  70                  75                  80  


Tyr Gln Ile Leu Lys Arg Gly Gly Leu Lys Asp Glu Asn Ile Val Val 
                85                  90                  95      


Phe Met Tyr Asp Asp Ile Ala Tyr Asn Glu Ser Asn Pro Arg Pro Gly 
            100                 105                 110         


Val Ile Ile Asn Ser Pro His Gly Ser Asp Val Tyr Ala Gly Val Pro 
        115                 120                 125             


Lys Asp Tyr Thr Gly Glu Glu Val Asn Ala Lys Asn Phe Leu Ala Ala 
    130                 135                 140                 


Ile Leu Gly Asn Lys Ser Ala Ile Thr Gly Gly Ser Gly Lys Val Val 
145                 150                 155                 160 


Asp Ser Gly Pro Asn Asp His Ile Phe Ile Tyr Tyr Thr Asp His Gly 
                165                 170                 175     


Ala Ala Gly Val Ile Gly Met Pro Ser Lys Pro Tyr Leu Tyr Ala Asp 
            180                 185                 190         


Glu Leu Asn Asp Ala Leu Lys Lys Lys His Ala Ser Gly Thr Tyr Lys 
        195                 200                 205             


Ser Leu Val Phe Tyr Leu Glu Ala Cys Glu Ser Gly Ser Met Phe Glu 
    210                 215                 220                 


Gly Ile Leu Pro Glu Asp Leu Asn Ile Tyr Ala Leu Thr Ser Thr Asn 
225                 230                 235                 240 


Thr Thr Glu Ser Ser Trp Ala Tyr Tyr Cys Pro Ala Gln Glu Asn Pro 
                245                 250                 255     


Pro Pro Pro Glu Tyr Asn Val Cys Leu Gly Asp Leu Phe Ser Val Ala 
            260                 265                 270         


Trp Leu Glu Asp Ser Asp Val Gln Asn Ser Trp Tyr Glu Thr Leu Asn 
        275                 280                 285             


Gln Gln Tyr His His Val Asp Lys Arg Ile Ser His Ala Ser His Ala 
    290                 295                 300                 


Thr Gln Tyr Gly Asn Leu Lys Leu Gly Glu Glu Gly Leu Phe Val Tyr 
305                 310                 315                 320 


Met Gly Ser Asn Pro Ala Asn Asp Asn Tyr Thr Ser Leu Asp Gly Asn 
                325                 330                 335     


Ala Leu Thr Pro Ser Ser Ile Val Val Asn Gln Arg Asp Ala Asp Leu 
            340                 345                 350         


Leu His Leu Trp Glu Lys Phe Arg Lys Ala Pro Glu Gly Ser Ala Arg 
        355                 360                 365             


Lys Glu Glu Ala Gln Thr Gln Ile Phe Lys Ala Met Ser His Arg Val 
    370                 375                 380                 


His Ile Asp Ser Ser Ile Lys Leu Ile Gly Lys Leu Leu Phe Gly Ile 
385                 390                 395                 400 


Glu Lys Cys Thr Glu Ile Leu Asn Ala Val Arg Pro Ala Gly Gln Pro 
                405                 410                 415     


Leu Val Asp Asp Trp Ala Cys Leu Arg Ser Leu Val Gly Thr Phe Glu 
            420                 425                 430         


Thr His Cys Gly Ser Leu Ser Glu Tyr Gly Met Arg His Thr Arg Thr 
        435                 440                 445             


Ile Ala Asn Ile Cys Asn Ala Gly Ile Ser Glu Glu Gln Met Ala Glu 
    450                 455                 460                 


Ala Ala Ser Gln Ala Cys Ala Ser Ile Pro 
465                 470                 


<210>  2
<211>  18
<212>  PRT
<213>  Ovaloparmena affinis


<220>
<221>  MISC_FEATURE
<222>  (1)..(1)
<223>  Xaa corresponds to position 247 of SEQ ID NO:1 and is Gly, Ala, 
       Ser, or Cys.

<220>
<221>  MISC_FEATURE
<222>  (2)..(2)
<223>  Xaa is any amino acid, preferably any amino acid that does not 
       disrupt the structural fold of the remaining conserved amino 
       acids

<220>
<221>  MISC_FEATURE
<222>  (6)..(6)
<223>  Xaa is any amino acid, preferably any amino acid that does not 
       disrupt the structural fold of the remaining conserved amino 
       acids

<220>
<221>  MISC_FEATURE
<222>  (7)..(7)
<223>  Xaa is any amino acid, preferably any amino acid that does not 
       disrupt the structural fold of the remaining conserved amino 
       acids

<220>
<221>  MISC_FEATURE
<222>  (8)..(8)
<223>  Xaa is any amino acid, preferably any amino acid that does not 
       disrupt the structural fold of the remaining conserved amino 
       acids

<220>
<221>  MISC_FEATURE
<222>  (9)..(9)
<223>  Xaa is any amino acid, preferably any amino acid that does not 
       disrupt the structural fold of the remaining conserved amino 
       acids

<220>
<221>  MISC_FEATURE
<222>  (10)..(10)
<223>  Xaa is any amino acid, preferably any amino acid that does not 
       disrupt the structural fold of the remaining conserved amino 
       acids

<220>
<221>  MISC_FEATURE
<222>  (16)..(16)
<223>  Xaa is any amino acid, preferably any amino acid that does not 
       disrupt the structural fold of the remaining conserved amino 
       acids

<220>
<221>  MISC_FEATURE
<222>  (17)..(17)
<223>  Xaa corresponds to position 263 of SEQ ID NO:1 and is any amino 
       acid, preferably any amino acid that does not disrupt the 
       structural fold of the remaining conserved amino acids

<400>  2

Xaa Xaa Tyr Cys Pro Xaa Xaa Xaa Xaa Xaa Pro Pro Pro Glu Tyr Xaa 
1               5                   10                  15      


Xaa Cys 
        


<210>  3
<211>  474
<212>  PRT
<213>  Ovaloparmena affinis

<400>  3

Met Val Arg Tyr Leu Ala Gly Ala Val Leu Leu Leu Val Val Leu Ser 
1               5                   10                  15      


Val Ala Ala Ala Val Ser Gly Ala Arg Asp Gly Asp Tyr Leu His Leu 
            20                  25                  30          


Pro Ser Glu Val Ser Arg Phe Phe Arg Pro Gln Glu Thr Asn Asp Asp 
        35                  40                  45              


His Gly Glu Asp Ser Val Gly Thr Arg Trp Ala Val Leu Ile Ala Gly 
    50                  55                  60                  


Ser Lys Gly Tyr Ala Asn Tyr Arg His Gln Ala Gly Val Cys His Ala 
65                  70                  75                  80  


Tyr Gln Ile Leu Lys Arg Gly Gly Leu Lys Asp Glu Asn Ile Val Val 
                85                  90                  95      


Phe Met Tyr Asp Asp Ile Ala Tyr Asn Glu Ser Asn Pro Arg Pro Gly 
            100                 105                 110         


Val Ile Ile Asn Ser Pro His Gly Ser Asp Val Tyr Ala Gly Val Pro 
        115                 120                 125             


Lys Asp Tyr Thr Gly Glu Glu Val Asn Ala Lys Asn Phe Leu Ala Ala 
    130                 135                 140                 


Ile Leu Gly Asn Lys Ser Ala Ile Thr Gly Gly Ser Gly Lys Val Val 
145                 150                 155                 160 


Asp Ser Gly Pro Asn Asp His Ile Phe Ile Tyr Tyr Thr Asp His Gly 
                165                 170                 175     


Ala Ala Gly Val Ile Gly Met Pro Ser Lys Pro Tyr Leu Tyr Ala Asp 
            180                 185                 190         


Glu Leu Asn Asp Ala Leu Lys Lys Lys His Ala Ser Gly Thr Tyr Lys 
        195                 200                 205             


Ser Leu Val Phe Tyr Leu Glu Ala Cys Glu Ser Gly Ser Met Phe Glu 
    210                 215                 220                 


Gly Ile Leu Pro Glu Asp Leu Asn Ile Tyr Ala Leu Thr Ser Thr Asn 
225                 230                 235                 240 


Thr Thr Glu Ser Ser Trp Cys Tyr Tyr Cys Pro Ala Gln Glu Asn Pro 
                245                 250                 255     


Pro Pro Pro Glu Tyr Asn Val Cys Leu Gly Asp Leu Phe Ser Val Ala 
            260                 265                 270         


Trp Leu Glu Asp Ser Asp Val Gln Asn Ser Trp Tyr Glu Thr Leu Asn 
        275                 280                 285             


Gln Gln Tyr His His Val Asp Lys Arg Ile Ser His Ala Ser His Ala 
    290                 295                 300                 


Thr Gln Tyr Gly Asn Leu Lys Leu Gly Glu Glu Gly Leu Phe Val Tyr 
305                 310                 315                 320 


Met Gly Ser Asn Pro Ala Asn Asp Asn Tyr Thr Ser Leu Asp Gly Asn 
                325                 330                 335     


Ala Leu Thr Pro Ser Ser Ile Val Val Asn Gln Arg Asp Ala Asp Leu 
            340                 345                 350         


Leu His Leu Trp Glu Lys Phe Arg Lys Ala Pro Glu Gly Ser Ala Arg 
        355                 360                 365             


Lys Glu Glu Ala Gln Thr Gln Ile Phe Lys Ala Met Ser His Arg Val 
    370                 375                 380                 


His Ile Asp Ser Ser Ile Lys Leu Ile Gly Lys Leu Leu Phe Gly Ile 
385                 390                 395                 400 


Glu Lys Cys Thr Glu Ile Leu Asn Ala Val Arg Pro Ala Gly Gln Pro 
                405                 410                 415     


Leu Val Asp Asp Trp Ala Cys Leu Arg Ser Leu Val Gly Thr Phe Glu 
            420                 425                 430         


Thr His Cys Gly Ser Leu Ser Glu Tyr Gly Met Arg His Thr Arg Thr 
        435                 440                 445             


Ile Ala Asn Ile Cys Asn Ala Gly Ile Ser Glu Glu Gln Met Ala Glu 
    450                 455                 460                 


Ala Ala Ser Gln Ala Cys Ala Ser Ile Pro 
465                 470                 


<210>  4
<211>  462
<212>  PRT
<213>  Clitoria ternatea

<400>  4

Ile Arg Asp Asp Phe Leu Arg Leu Pro Ser Gln Ala Ser Lys Phe Phe 
1               5                   10                  15      


Gln Ala Asp Asp Asn Val Glu Gly Thr Arg Trp Ala Val Leu Val Ala 
            20                  25                  30          


Gly Ser Lys Gly Tyr Val Asn Tyr Arg His Gln Ala Asp Val Cys His 
        35                  40                  45              


Ala Tyr Gln Ile Leu Lys Lys Gly Gly Leu Lys Asp Glu Asn Ile Ile 
    50                  55                  60                  


Val Phe Met Tyr Asp Asp Ile Ala Tyr Asn Glu Ser Asn Pro His Pro 
65                  70                  75                  80  


Gly Val Ile Ile Asn His Pro Tyr Gly Ser Asp Val Tyr Lys Gly Val 
                85                  90                  95      


Pro Lys Asp Tyr Val Gly Glu Asp Ile Asn Pro Pro Asn Phe Tyr Ala 
            100                 105                 110         


Val Leu Leu Ala Asn Lys Ser Ala Leu Thr Gly Thr Gly Ser Gly Lys 
        115                 120                 125             


Val Leu Asp Ser Gly Pro Asn Asp His Val Phe Ile Tyr Tyr Thr Asp 
    130                 135                 140                 


His Gly Gly Ala Gly Val Leu Gly Met Pro Ser Lys Pro Tyr Ile Ala 
145                 150                 155                 160 


Ala Ser Asp Leu Asn Asp Val Leu Lys Lys Lys His Ala Ser Gly Thr 
                165                 170                 175     


Tyr Lys Ser Ile Val Phe Tyr Val Glu Ser Cys Glu Ser Gly Ser Met 
            180                 185                 190         


Phe Asp Gly Leu Leu Pro Glu Asp His Asn Ile Tyr Val Met Gly Ala 
        195                 200                 205             


Ser Asp Thr Gly Glu Ser Ser Trp Val Thr Tyr Cys Pro Leu Gln His 
    210                 215                 220                 


Pro Ser Pro Pro Pro Glu Tyr Asp Val Cys Val Gly Asp Leu Phe Ser 
225                 230                 235                 240 


Val Ala Trp Leu Glu Asp Cys Asp Val His Asn Leu Gln Thr Glu Thr 
                245                 250                 255     


Phe Gln Gln Gln Tyr Glu Val Val Lys Asn Lys Thr Ile Val Ala Leu 
            260                 265                 270         


Ile Glu Asp Gly Thr His Val Val Gln Tyr Gly Asp Val Gly Leu Ser 
        275                 280                 285             


Lys Gln Thr Leu Phe Val Tyr Met Gly Thr Asp Pro Ala Asn Asp Asn 
    290                 295                 300                 


Asn Thr Phe Thr Asp Lys Asn Ser Leu Gly Thr Pro Arg Lys Ala Val 
305                 310                 315                 320 


Ser Gln Arg Asp Ala Asp Leu Ile His Tyr Trp Glu Lys Tyr Arg Arg 
                325                 330                 335     


Ala Pro Glu Gly Ser Ser Arg Lys Ala Glu Ala Lys Lys Gln Leu Arg 
            340                 345                 350         


Glu Val Met Ala His Arg Met His Ile Asp Asn Ser Val Lys His Ile 
        355                 360                 365             


Gly Lys Leu Leu Phe Gly Ile Glu Lys Gly His Lys Met Leu Asn Asn 
    370                 375                 380                 


Val Arg Pro Ala Gly Leu Pro Val Val Asp Asp Trp Asp Cys Phe Lys 
385                 390                 395                 400 


Thr Leu Ile Arg Thr Phe Glu Thr His Cys Gly Ser Leu Ser Glu Tyr 
                405                 410                 415     


Gly Met Lys His Met Arg Ser Phe Ala Asn Leu Cys Asn Ala Gly Ile 
            420                 425                 430         


Arg Lys Glu Gln Met Ala Glu Ala Ser Ala Gln Ala Cys Val Ser Ile 
        435                 440                 445             


Pro Asp Asn Pro Trp Ser Ser Leu His Ala Gly Phe Ser Val 
    450                 455                 460         


<210>  5
<211>  488
<212>  PRT
<213>  Coffea canephora

<400>  5

Met Met Arg Tyr Ala Thr Ala Ala Leu Leu Leu Leu Ala Leu Ser Ile 
1               5                   10                  15      


Ile Ala Val Ala Glu Ala Arg Asp Asn Phe Leu Lys Leu Pro Ser Glu 
            20                  25                  30          


Ile Ala Asp Phe Phe His Pro Lys Glu Arg Ser Asp Ala Gly Gly Asp 
        35                  40                  45              


Ser Val Gly Thr Arg Trp Ala Val Leu Ile Ala Gly Ser Asn Gly Tyr 
    50                  55                  60                  


Trp Asn Tyr Arg His Gln Ala Asp Val Cys His Ala Tyr Gln Ile Leu 
65                  70                  75                  80  


Lys Arg Gly Gly Leu Lys Asp Glu Asn Ile Val Val Phe Met Tyr Asp 
                85                  90                  95      


Asp Ile Ala Tyr Asn Glu Glu Asn Pro Arg Pro Gly Val Ile Ile Asn 
            100                 105                 110         


Ser Pro His Gly Ala Asp Val Tyr Gln Gly Val Pro Lys Asp Tyr Thr 
        115                 120                 125             


Gly Asp Asp Val Asn Ala Lys Asn Phe Leu Ala Ala Ile Leu Gly Asp 
    130                 135                 140                 


Lys Thr Ala Ile Thr Gly Gly Ser Gly Lys Val Val Asp Ser Gly Pro 
145                 150                 155                 160 


Asn Asp His Ile Phe Ile Tyr Tyr Thr Asp His Gly Gly Pro Gly Val 
                165                 170                 175     


Leu Gly Thr Pro Ser Gly Pro Tyr Leu Tyr Ala Asp Asp Leu Asn Glu 
            180                 185                 190         


Val Leu Lys Lys Lys His Ala Ser Gly Thr Tyr Lys Ser Leu Val Phe 
        195                 200                 205             


Tyr Leu Glu Ala Cys Glu Ser Gly Ser Ile Phe Glu Gly Leu Leu Pro 
    210                 215                 220                 


Glu Asp Leu Asn Ile Tyr Ala Thr Thr Ala Ser Asn Ala Glu Glu Ser 
225                 230                 235                 240 


Ser Trp Gly Thr Tyr Cys Pro Gly Glu Tyr Pro Ser Pro Pro Pro Glu 
                245                 250                 255     


Tyr Glu Thr Cys Leu Gly Asp Leu Tyr Ser Val Ala Trp Met Glu Asp 
            260                 265                 270         


Ser Glu Ile His Asn Leu His Thr Glu Thr Leu Lys Gln Gln Tyr His 
        275                 280                 285             


Leu Val Lys Lys Arg Thr Ser Ser Ser Asn Ser Ala Tyr Gly Ser His 
    290                 295                 300                 


Val Met Gln Tyr Gly Asp Leu Lys Leu Ser Leu Glu Asp Leu Phe Leu 
305                 310                 315                 320 


Tyr Met Gly Thr Asn Pro Ala Asn Asp Asn Tyr Thr Phe Val Asp Glu 
                325                 330                 335     


Asn Ser Leu Arg Pro Ser Ser Lys Ala Val Asn Gln Arg Asp Ala Asp 
            340                 345                 350         


Leu Leu His Phe Trp Asp Lys Phe Arg Lys Ala Pro Glu Gly Ser Ala 
        355                 360                 365             


Arg Lys Val Glu Ala Gln Lys Gln Val Val Glu Ala Met Ser His Arg 
    370                 375                 380                 


Met His Ile Asp Asn Ser Val Lys Leu Ile Gly Lys Leu Leu Phe Gly 
385                 390                 395                 400 


Ile Glu Lys Gly Ser Glu Ile Leu Asn Ser Val Arg Pro Ala Gly His 
                405                 410                 415     


Pro Leu Ala Asp Asp Trp Asp Cys Leu Lys Ser Leu Val Arg Thr Phe 
            420                 425                 430         


Glu Thr His Cys Gly Ser Leu Ser Gln Tyr Gly Met Lys His Met Arg 
        435                 440                 445             


Ser Ile Ala Asn Ile Cys Asn Ala Gly Ile Lys Lys Asp Gln Met Ala 
    450                 455                 460                 


Glu Ala Ala Ala Gln Ala Cys Val Ser Leu Pro Ser Asn Ser Trp Ser 
465                 470                 475                 480 


Ser Leu His Arg Gly Phe Ser Ala 
                485             


<210>  6
<211>  491
<212>  PRT
<213>  Daucus carota subsp. sativus

<400>  6

Met Val Arg Tyr Leu Ala Gly Ala Val Ala Leu Leu Val Val Val Ser 
1               5                   10                  15      


Ile Ser Ala Val Val Glu Ser Arg Arg Asp Ile Val Gly Asp Val Leu 
            20                  25                  30          


Lys Leu Pro Ser Glu Val Ser Ser Phe Phe Arg Pro Val Ala Glu Glu 
        35                  40                  45              


Glu Asp Ser Val Gly Thr Arg Trp Ala Val Leu Ile Ala Gly Ser Asn 
    50                  55                  60                  


Gly Tyr Trp Asn Tyr Arg His Gln Ala Asp Ile Cys His Ala Tyr Gln 
65                  70                  75                  80  


Leu Leu Arg Arg Gly Gly Val Lys Glu Glu Asn Ile Val Val Phe Met 
                85                  90                  95      


Tyr Asp Asp Ile Ala Tyr Asp Glu Glu Asn Pro Arg Pro Gly Val Ile 
            100                 105                 110         


Ile Asn Ser Pro His Gly Ser Asp Val Tyr Lys Gly Val Pro Lys Asp 
        115                 120                 125             


Tyr Thr Gly Glu Asp Val Thr Val Asn Asn Val Phe Ala Ala Ile Leu 
    130                 135                 140                 


Gly Asp Lys Ser Ala Thr Thr Gly Gly Ser Gly Lys Val Val Asp Ser 
145                 150                 155                 160 


Gly Pro Asn Asp His Ile Phe Ile Tyr Tyr Ser Asp His Gly Gly Pro 
                165                 170                 175     


Gly Val Leu Gly Met Pro Thr Asn Pro Tyr Met Tyr Ala Gly Asp Leu 
            180                 185                 190         


Val Asp Val Leu Lys Lys Lys His Ala Ser Gly Thr Tyr Lys Ser Met 
        195                 200                 205             


Val Phe Tyr Leu Glu Ala Cys Glu Ser Gly Ser Ile Phe Glu Gly Leu 
    210                 215                 220                 


Leu Pro Glu Gly Leu Asn Ile Tyr Ala Thr Thr Ala Ser Asn Ala Tyr 
225                 230                 235                 240 


Glu Ser Ser Trp Gly Thr Tyr Cys Pro Gly Glu Tyr Pro Ser Pro Pro 
                245                 250                 255     


Pro Glu Tyr Glu Thr Cys Leu Gly Asp Leu Tyr Ser Val Ala Trp Met 
            260                 265                 270         


Glu Asp Ser Asp Ile His Asn Leu Arg Thr Glu Thr Leu Arg Gln Gln 
        275                 280                 285             


Tyr Gln Gln Val Lys Lys Arg Thr Ser Asn Asp Asn Ser Gly Trp Gly 
    290                 295                 300                 


Ser His Val Met Gln Tyr Gly Asp Leu Lys Leu Ser Thr Glu Glu Leu 
305                 310                 315                 320 


Phe Met Tyr Met Gly Thr Asn Pro Ala Asn Asp Asn Phe Thr Phe Val 
                325                 330                 335     


Asp Asp Asn Ser Leu Arg Leu Ser Ser Ser Lys Ala Val Asn Gln Arg 
            340                 345                 350         


Asp Ala Asp Leu Leu His Phe Trp Asp Lys Tyr Arg Lys Ala Pro Glu 
        355                 360                 365             


Gly Ser Asp Arg Lys Ile Ala Ala Gln Lys Gln Phe Ser Glu Ala Met 
    370                 375                 380                 


Ser His Arg Met His Leu Asp Asn Ser Ile Gln Leu Ile Gly Lys Leu 
385                 390                 395                 400 


Leu Phe Gly Ile Asp Thr Ala Ser Glu Val Leu Thr Thr Val Arg Pro 
                405                 410                 415     


Ser Gly Gln Pro Leu Val Asp Asp Trp Leu Cys Leu Lys Lys Leu Val 
            420                 425                 430         


Arg Thr Phe Glu Thr Tyr Cys Gly Ser Leu Ser Gln Tyr Gly Met Lys 
        435                 440                 445             


His Met Arg Ser Ile Ala Asn Ile Cys Asn Ala Gly Ile Ser Glu Glu 
    450                 455                 460                 


Gln Met Ser Glu Ala Ser Ala Gln Ala Cys Val Thr Phe Pro Ser Asn 
465                 470                 475                 480 


Pro Trp Ser Ser Val Asn Lys Gly Phe Thr Ala 
                485                 490     


<210>  7
<211>  486
<212>  PRT
<213>  Corchorus capsularis

<400>  7

Met Thr Arg Leu Val Ala Gly Val Ile Leu Leu Leu Leu Ser Val Thr 
1               5                   10                  15      


Gly Ile Val Ser Ala Gly Arg Asp Ala Thr Gly Asp Val Leu Arg Leu 
            20                  25                  30          


Pro Ser Glu Ala Ser Arg Phe Phe Arg Pro Ser Asp Asp Asp Glu Val 
        35                  40                  45              


Gly Thr Arg Trp Ala Val Leu Ile Ala Gly Ser Asn Gly Tyr Trp Asn 
    50                  55                  60                  


Tyr Arg His Gln Ala Asp Val Cys His Ala Tyr Gln Leu Leu Lys Lys 
65                  70                  75                  80  


Gly Gly Leu Lys Asp Glu Asn Ile Ile Val Phe Met Tyr Asp Asp Ile 
                85                  90                  95      


Ala Tyr Asn Tyr Glu Asn Pro Arg Gln Gly Ile Ile Ile Asn Ser Pro 
            100                 105                 110         


His Gly Asp Asp Val Tyr Gln Gly Val Pro Lys Asp Tyr Thr Gly Glu 
        115                 120                 125             


Glu Val Thr Val His Asn Phe Leu Ala Ala Ile Leu Gly Asn Lys Thr 
    130                 135                 140                 


Ala Ile Thr Gly Gly Ser Gly Lys Val Val Asp Ser Gly Pro Asn Asp 
145                 150                 155                 160 


His Ile Phe Ile Tyr Tyr Thr Asp His Gly Gly Pro Gly Val Leu Gly 
                165                 170                 175     


Met Pro Thr Tyr Pro Tyr Leu Tyr Ala Asp Glu Leu Ile Asp Thr Leu 
            180                 185                 190         


Lys Lys Lys His Ala Ser Gly Thr Tyr Lys Ser Leu Val Phe Tyr Leu 
        195                 200                 205             


Glu Ala Cys Glu Ser Gly Ser Ile Phe Glu Gly Leu Leu Pro Glu Gly 
    210                 215                 220                 


Leu Asn Ile Tyr Ala Thr Thr Ala Ser Asn Ala Asp Glu Ser Ser Trp 
225                 230                 235                 240 


Gly Thr Tyr Cys Pro Gly Glu Tyr Pro Ser Pro Pro Pro Glu Tyr Glu 
                245                 250                 255     


Thr Cys Leu Gly Asp Leu Tyr Ser Val Ala Trp Met Glu Asp Ser Asp 
            260                 265                 270         


Leu His Asn Leu Arg Thr Glu Thr Leu His Gln Gln Tyr Glu Leu Val 
        275                 280                 285             


Lys Arg Arg Thr Leu Asn Gly Asn Ser Ala Tyr Gly Ser His Val Met 
    290                 295                 300                 


Gln Tyr Gly Asp Val Gly Leu Ala Lys Glu His Leu Phe Leu Tyr Leu 
305                 310                 315                 320 


Gly Thr Asn Pro Ala Asn Asp Asn Phe Thr Phe Ile Asp Glu Asn Ser 
                325                 330                 335     


Leu Gln Pro Pro Ala Lys Ala Val Asn Gln Arg Asp Ala Asp Leu Val 
            340                 345                 350         


His Phe Trp Asp Lys Tyr Arg Lys Ala Pro Asp Gly Ser Ala Arg Lys 
        355                 360                 365             


Val Glu Ala Gln Lys Gln Val Val Glu Ala Met Ser His Arg Met His 
    370                 375                 380                 


Val Asp Asn Ser Ile Gln Leu Ile Gly Lys Leu Leu Phe Gly Ile Glu 
385                 390                 395                 400 


Arg Gly Ala Asp Val Leu Lys Thr Val Arg Pro Ala Gly Gln Pro Leu 
                405                 410                 415     


Val Asp Asp Trp Lys Cys Leu Lys Ser Met Val Arg Thr Phe Glu Thr 
            420                 425                 430         


His Cys Gly Ser Leu Ala Gln Tyr Gly Met Lys His Met Arg Ser Ile 
        435                 440                 445             


Ala Asn Ile Cys Asn Ala Gly Ile Gln Thr Glu Gln Ile Ala Glu Ala 
    450                 455                 460                 


Ser Ala Gln Ala Cys Val Ser Ile Pro Ser Gly Gln Trp Ser Ser Ile 
465                 470                 475                 480 


Gln Lys Gly Phe Ser Ala 
                485     


<210>  8
<211>  486
<212>  PRT
<213>  Corchorus olitorius

<400>  8

Met Thr Arg Leu Val Ala Gly Val Ile Leu Leu Leu Leu Ser Val Thr 
1               5                   10                  15      


Gly Ile Ala Ser Ala Gly Arg Asp Ala Thr Gly Asp Val Leu Arg Leu 
            20                  25                  30          


Pro Ser Glu Ala Ser Arg Phe Phe Arg Pro Ser Asp Asp Asp Glu Val 
        35                  40                  45              


Gly Thr Arg Trp Ala Val Leu Ile Ala Gly Ser Asn Gly Tyr Trp Asn 
    50                  55                  60                  


Tyr Arg His Gln Ala Asp Val Cys His Ala Tyr Gln Leu Leu Lys Lys 
65                  70                  75                  80  


Gly Gly Leu Lys Asp Glu Asn Ile Ile Val Phe Met Tyr Asp Asp Ile 
                85                  90                  95      


Ala Phe Asn Tyr Glu Asn Pro Arg Gln Gly Ile Ile Ile Asn Ser Pro 
            100                 105                 110         


His Gly Asp Asp Val Tyr Gln Asp Val Pro Lys Asp Tyr Thr Gly Glu 
        115                 120                 125             


Glu Val Thr Val His Asn Phe Leu Ala Ala Ile Leu Gly Asn Lys Thr 
    130                 135                 140                 


Ala Ile Lys Gly Gly Ser Gly Lys Val Val Asp Ser Gly Pro Asn Asp 
145                 150                 155                 160 


His Ile Phe Ile Tyr Tyr Thr Asp His Gly Gly Pro Gly Val Leu Gly 
                165                 170                 175     


Met Pro Thr Tyr Pro Tyr Leu Tyr Ala Asp Glu Leu Ile Asp Thr Leu 
            180                 185                 190         


Lys Lys Lys His Ala Ser Gly Thr Tyr Lys Ser Leu Val Phe Tyr Leu 
        195                 200                 205             


Glu Ala Cys Glu Ser Gly Ser Ile Phe Glu Gly Leu Leu Pro Glu Gly 
    210                 215                 220                 


Leu Asn Ile Tyr Ala Thr Thr Ala Ser Asn Ala Asp Glu Ser Ser Trp 
225                 230                 235                 240 


Gly Thr Tyr Cys Pro Gly Glu Tyr Pro Ser Pro Pro Pro Glu Tyr Glu 
                245                 250                 255     


Thr Cys Leu Gly Asp Leu Tyr Ser Val Ala Trp Met Glu Asp Ser Asp 
            260                 265                 270         


Leu His Asn Leu Arg Thr Glu Thr Leu His Gln Gln Tyr Glu Leu Val 
        275                 280                 285             


Lys Arg Arg Thr Leu Asn Gly Asn Ser Ala Tyr Gly Ser His Val Met 
    290                 295                 300                 


Gln Tyr Gly Asp Val Gly Leu Ala Lys Glu His Leu Phe Leu Tyr Leu 
305                 310                 315                 320 


Gly Thr Asn Pro Ala Asn Asp Asn Phe Thr Phe Ile Asp Glu Asn Ser 
                325                 330                 335     


Leu Gln Pro Pro Ala Lys Ala Val Asn Gln Arg Asp Ala Asp Leu Val 
            340                 345                 350         


His Phe Trp Asp Lys Tyr Arg Lys Ala Pro Asp Gly Ser Val Arg Lys 
        355                 360                 365             


Val Glu Ala Gln Lys Gln Val Val Glu Ala Met Ser His Arg Met His 
    370                 375                 380                 


Val Asp Asn Ser Ile Gln Leu Ile Gly Lys Leu Leu Phe Gly Ile Glu 
385                 390                 395                 400 


Arg Gly Ala Asp Val Leu Lys Thr Val Arg Pro Ala Gly Gln Pro Leu 
                405                 410                 415     


Val Asp Asp Trp Lys Cys Leu Lys Ser Met Val Arg Thr Phe Glu Thr 
            420                 425                 430         


His Cys Gly Ser Leu Ala Gln Tyr Gly Met Lys His Met Arg Ser Ile 
        435                 440                 445             


Ala Asn Ile Cys Asn Ala Gly Ile Gln Thr Glu Gln Met Ala Glu Ala 
    450                 455                 460                 


Ser Ser Gln Ala Cys Val Ser Ile Pro Ser Gly Gln Trp Ser Ser Ile 
465                 470                 475                 480 


Gln Lys Gly Phe Ser Ala 
                485     


<210>  9
<211>  493
<212>  PRT
<213>  Jatropha curcas

<400>  9

Met Thr Arg Leu Ala Thr Gly Val Ile Leu Leu Leu Leu Ala Leu Cys 
1               5                   10                  15      


Val Val Ser Ser Ala Gly Ser Arg Asp Ile Val Gly Asp Val Leu Arg 
            20                  25                  30          


Leu Pro Ser Glu Ala Ser Arg Phe Phe Arg Pro Gly Gly Ala His Val 
        35                  40                  45              


Ala Lys Glu Asp Asp Ser Thr Gly Thr Arg Trp Ala Ile Leu Ile Ala 
    50                  55                  60                  


Gly Ser Asn Gly Tyr Trp Asn Tyr Arg His Gln Ala Asp Val Cys His 
65                  70                  75                  80  


Ala Tyr Gln Leu Leu Lys Lys Gly Gly Leu Lys Asp Glu Asn Ile Ile 
                85                  90                  95      


Val Phe Met Tyr Asp Asp Ile Ala Phe Asn Lys Glu Asn Pro Arg Pro 
            100                 105                 110         


Gly Val Ile Ile Asn Asn Pro Tyr Gly Glu Asp Val Tyr Lys Gly Val 
        115                 120                 125             


Pro Lys Asp Tyr Thr Gly Glu Asp Val Asn Val Asn Asn Phe Phe Ala 
    130                 135                 140                 


Ala Ile Leu Gly Asn Lys Thr Ala Ile Thr Gly Gly Ser Gly Lys Val 
145                 150                 155                 160 


Val Asp Ser Gly Pro Asn Asp His Ile Phe Ile Tyr Tyr Thr Asp His 
                165                 170                 175     


Gly Gly Pro Gly Val Leu Gly Met Pro Thr Asn Pro Tyr Leu Tyr Ala 
            180                 185                 190         


Asn Asp Leu Ile Asp Val Leu Ile Lys Lys His Ala Ser Gly Thr Tyr 
        195                 200                 205             


Lys Ser Leu Val Phe Tyr Leu Glu Ala Cys Glu Ser Gly Ser Ile Phe 
    210                 215                 220                 


Glu Gly Leu Leu Pro Glu Gly Leu Asn Ile Tyr Ala Thr Thr Ala Ala 
225                 230                 235                 240 


Asn Ala Glu Glu Ser Ser Trp Gly Thr Tyr Cys Pro Gly Glu Tyr Pro 
                245                 250                 255     


Ser Pro Pro Pro Glu Tyr Glu Thr Cys Leu Gly Asp Leu Tyr Ser Val 
            260                 265                 270         


Ala Trp Met Glu Asp Ser Asp Val His Asn Leu Gln Thr Glu Thr Leu 
        275                 280                 285             


Arg Gln Gln Tyr Gln Leu Val Lys Arg Arg Thr Ala Asn Gly Asn Ser 
    290                 295                 300                 


Ala Tyr Gly Ser His Val Met Gln Tyr Gly Asp Val Gly Leu Ser Lys 
305                 310                 315                 320 


Asp Asn Leu Phe Leu Tyr Met Gly Thr Asn Pro Ala Asn Glu Asn Tyr 
                325                 330                 335     


Thr Phe Val Asp Glu Asn Ser Leu Arg Pro Pro Ser Lys Ala Val Asn 
            340                 345                 350         


Gln Arg Asp Ala Asp Leu Val His Phe Trp Asp Lys Tyr Arg Lys Ala 
        355                 360                 365             


Pro Asp Gly Ser Thr Arg Lys Ile Gln Ala Gln Lys Gln Phe Val Glu 
    370                 375                 380                 


Ala Met Ser His Arg Met His Leu Asp His Ser Met Lys Leu Ile Gly 
385                 390                 395                 400 


Lys Leu Leu Phe Gly Ile Gly Lys Gly Ser Glu Val Leu Asn Ala Ile 
                405                 410                 415     


Arg Pro Ala Gly Gln Pro Leu Val Asp Asp Trp Val Cys Leu Lys Thr 
            420                 425                 430         


Leu Val Arg Thr Phe Glu Thr His Cys Gly Ser Leu Ser Gln Tyr Gly 
        435                 440                 445             


Met Lys His Met Arg Ser Leu Ala Asn Leu Cys Asn Ala Gly Ile Arg 
    450                 455                 460                 


Glu Asp Gln Met Ala Glu Ala Ser Ala Gln Ala Cys Val Ser Ile Pro 
465                 470                 475                 480 


Ser Gly Pro Trp Ser Ser Leu His Lys Gly Phe Ser Ala 
                485                 490             


<210>  10
<211>  494
<212>  PRT
<213>  Malus hupehensis

<400>  10

Met Thr Arg Leu Ala Ser Ala Val Val Leu Leu Phe Leu Ala Ser Val 
1               5                   10                  15      


Leu Ala Ser Ala Ala Gly Ser Arg Asp Leu Ile Gly Asp Val Leu Arg 
            20                  25                  30          


Leu Pro Ser Glu Ala Ser Arg Phe Phe Gly Arg Gly Asp Asp Ala Pro 
        35                  40                  45              


Asp Gln Gln Asp Asp Gly Thr Val Gly Thr Arg Trp Ala Val Leu Ile 
    50                  55                  60                  


Ala Gly Ser Asn Gly Tyr Trp Asn Tyr Arg His Gln Ala Asp Ile Cys 
65                  70                  75                  80  


His Ala Tyr Gln Leu Leu Lys Lys Gly Gly Leu Lys Asp Glu Asn Ile 
                85                  90                  95      


Val Val Phe Met Tyr Asp Asp Ile Ala Tyr Asn Glu Glu Asn Pro Arg 
            100                 105                 110         


Gln Gly Val Ile Ile Asn Ser Pro His Gly Ser Asp Val Tyr Glu Gly 
        115                 120                 125             


Val Pro Lys Asp Tyr Thr Gly Glu Asp Val Thr Val Asn Asn Phe Phe 
    130                 135                 140                 


Ala Ala Ile Leu Gly Asn Lys Thr Ala Leu Thr Gly Gly Ser Gly Lys 
145                 150                 155                 160 


Val Val Asp Ser Gly Pro Asn Asp His Ile Phe Ile Tyr Tyr Thr Asp 
                165                 170                 175     


His Gly Gly Pro Gly Ile Leu Gly Met Pro Thr Ser Pro Tyr Ile Tyr 
            180                 185                 190         


Ala Asn Asp Leu Ile Glu Val Leu Lys Lys Lys His Ala Ala Gly Thr 
        195                 200                 205             


Tyr Arg Ser Leu Val Phe Tyr Leu Glu Ala Cys Glu Ser Gly Ser Ile 
    210                 215                 220                 


Phe Glu Gly Leu Leu Pro Glu Gly Leu Asn Ile Phe Ala Thr Thr Ala 
225                 230                 235                 240 


Ser Asn Ala Glu Glu Ser Ser Trp Gly Thr Tyr Cys Pro Gly Glu Tyr 
                245                 250                 255     


Pro Ser Pro Pro Pro Glu Tyr Asp Thr Cys Leu Gly Asp Leu Tyr Ser 
            260                 265                 270         


Val Ala Trp Met Glu Asp Ser Asp Val His Asn Leu Arg Ser Glu Thr 
        275                 280                 285             


Leu His Gln Gln Tyr Glu Leu Val Lys Thr Arg Ala Ala Asn Asp Asn 
    290                 295                 300                 


Ser Gly Ser Gly Ser His Val Met Gln Tyr Gly Asp Val Gly Leu Ser 
305                 310                 315                 320 


Lys Asn Asn Leu Phe Val Tyr Met Gly Thr Asn Pro Ala Asn Asp Asn 
                325                 330                 335     


Tyr Thr Phe Leu Gly Glu Asn Ser Leu Arg Pro Ser Ser Lys Ala Val 
            340                 345                 350         


Asn Gln Arg Asp Ala Asp Leu Leu His Phe Trp His Lys Tyr Arg Lys 
        355                 360                 365             


Ala Pro Glu Gly Ser Ala Arg Lys Ile Gln Ala Gln Lys Asp Phe Val 
    370                 375                 380                 


Glu Ala Met Ser His Arg Met His Ile Asp Gln Thr Met Lys Leu Ile 
385                 390                 395                 400 


Gly Lys Leu Leu Phe Gly Ile Glu Lys Gly Pro Gln Val Leu Asn Ala 
                405                 410                 415     


Val Arg Pro Ala Gly Gln Pro Leu Val Asp Asp Trp Asp Cys Leu Lys 
            420                 425                 430         


Thr Met Val Arg Ser Phe Glu Thr His Cys Gly Ser Leu Ser Gln Tyr 
        435                 440                 445             


Gly Met Lys His Met Arg Ser Leu Ala Asn Ile Cys Asn Ala Gly Met 
    450                 455                 460                 


Thr Gln Glu Gln Met Ala Glu Ala Ser Ala Gln Ala Cys Val Ser Ala 
465                 470                 475                 480 


Pro Ser Gly Arg Trp Ser Ser Leu His Arg Gly Phe Ser Ala 
                485                 490                 


<210>  11
<211>  494
<212>  PRT
<213>  Pyrus x bretschneideri

<400>  11

Met Thr Arg Phe Ala Gly Ala Val Val Leu Leu Phe Leu Ala Ser Val 
1               5                   10                  15      


Leu Ala Ser Ala Ala Gly Ser Arg Asp Leu Ile Gly Asp Val Leu Arg 
            20                  25                  30          


Leu Pro Ser Glu Ala Ser Arg Phe Phe Gly Arg Gly Asp Asp Ala Pro 
        35                  40                  45              


Asp Gln Gln Asp Asp Gly Thr Val Gly Thr Arg Trp Ala Val Leu Ile 
    50                  55                  60                  


Ala Gly Ser Asn Gly Tyr Trp Asn Tyr Arg His Gln Ala Asp Ile Cys 
65                  70                  75                  80  


His Ala Tyr Gln Leu Leu Lys Lys Gly Gly Leu Lys Asp Glu Asn Ile 
                85                  90                  95      


Val Val Phe Met Tyr Asp Asp Ile Ala Tyr Asn Glu Glu Asn Pro Arg 
            100                 105                 110         


Gln Gly Val Ile Ile Asn Asn Pro Gln Gly Ser Asp Val Tyr Glu Gly 
        115                 120                 125             


Val Pro Lys Asp Tyr Thr Gly Glu Asp Val Thr Val Asn Asn Phe Phe 
    130                 135                 140                 


Ala Ala Ile Leu Gly Asn Lys Thr Ala Leu Thr Gly Gly Ser Gly Lys 
145                 150                 155                 160 


Val Val Asp Ser Gly Pro Asn Asp His Ile Phe Ile Tyr Tyr Thr Asp 
                165                 170                 175     


His Gly Gly Pro Gly Val Leu Gly Met Pro Thr Ser Pro Tyr Ile Tyr 
            180                 185                 190         


Ala Asn Asp Leu Ile Glu Val Leu Lys Lys Lys His Ala Ala Gly Thr 
        195                 200                 205             


Tyr Lys Ser Leu Val Phe Tyr Leu Glu Ala Cys Glu Ser Gly Ser Ile 
    210                 215                 220                 


Phe Glu Gly Leu Leu Pro Glu Gly Leu Asn Ile Phe Ala Thr Thr Ala 
225                 230                 235                 240 


Ser Asn Ala Glu Glu Ser Ser Trp Gly Thr Tyr Cys Pro Gly Glu Tyr 
                245                 250                 255     


Pro Ser Pro Pro Pro Glu Tyr Glu Thr Cys Leu Gly Asp Leu Tyr Ser 
            260                 265                 270         


Val Ala Trp Met Glu Asp Ser Asp Ile His Asn Leu Arg Ser Glu Thr 
        275                 280                 285             


Leu His Gln Gln Tyr Glu Leu Val Lys Thr Arg Thr Ala Asn Asp Asn 
    290                 295                 300                 


Tyr Gly Phe Gly Ser His Val Met Gln Tyr Gly Asp Val Gly Leu Ser 
305                 310                 315                 320 


Lys Asn Asn Leu Phe Val Tyr Met Gly Thr Asn Pro Ala Asn Asp Asn 
                325                 330                 335     


Tyr Thr Phe Leu Gly Glu Asn Ser Leu Arg Pro Ser Ser Lys Ala Val 
            340                 345                 350         


Asn Gln Arg Asp Ala Asp Leu Leu His Phe Trp His Lys Tyr Arg Lys 
        355                 360                 365             


Ala Pro Glu Gly Ser Ala Arg Lys Val Gln Ala Gln Lys Asp Phe Val 
    370                 375                 380                 


Glu Ala Met Ser His Arg Met His Ile Asp Gln Thr Met Lys Leu Ile 
385                 390                 395                 400 


Gly Lys Leu Leu Phe Gly Ile Glu Lys Gly Pro Gln Val Leu Asn Ala 
                405                 410                 415     


Val Arg Pro Ala Gly Gln Pro Leu Val Asp Asp Trp Asp Cys Leu Lys 
            420                 425                 430         


Thr Met Val Arg Ser Phe Glu Thr His Cys Gly Ser Leu Ser Gln Tyr 
        435                 440                 445             


Gly Met Lys His Met Arg Ser Leu Ala Asn Ile Cys Asn Ala Gly Met 
    450                 455                 460                 


Thr Gln Glu Gln Met Ala Glu Ala Ser Ala Gln Ala Cys Val Ser Ala 
465                 470                 475                 480 


Pro Ser Ser Arg Trp Ser Ser Leu His Arg Gly Phe Ser Ala 
                485                 490                 


<210>  12
<211>  537
<212>  PRT
<213>  artificial sequence

<220>
<223>  artificial sequence

<400>  12

Met Gly Met Ala His His His His His His Met Gln Ile Phe Val Lys 
1               5                   10                  15      


Thr Leu Thr Gly Lys Thr Ile Thr Leu Glu Val Glu Pro Ser Asp Thr 
            20                  25                  30          


Ile Glu Asn Val Lys Ala Lys Ile Gln Asp Lys Glu Gly Ile Pro Pro 
        35                  40                  45              


Asp Gln Gln Arg Leu Ile Phe Ala Gly Lys Gln Leu Glu Asp Gly Arg 
    50                  55                  60                  


Thr Leu Ser Asp Tyr Asn Ile Gln Lys Glu Ser Thr Leu His Leu Val 
65                  70                  75                  80  


Leu Arg Leu Arg Gly Gly Ala Arg Asp Gly Asp Tyr Leu His Leu Pro 
                85                  90                  95      


Ser Glu Val Ser Arg Phe Phe Arg Pro Gln Glu Thr Asn Asp Asp His 
            100                 105                 110         


Gly Glu Asp Ser Val Gly Thr Arg Trp Ala Val Leu Ile Ala Gly Ser 
        115                 120                 125             


Lys Gly Tyr Ala Asn Tyr Arg His Gln Ala Gly Val Cys His Ala Tyr 
    130                 135                 140                 


Gln Ile Leu Lys Arg Gly Gly Leu Lys Asp Glu Asn Ile Val Val Phe 
145                 150                 155                 160 


Met Tyr Asp Asp Ile Ala Tyr Asn Glu Ser Asn Pro Arg Pro Gly Val 
                165                 170                 175     


Ile Ile Asn Ser Pro His Gly Ser Asp Val Tyr Ala Gly Val Pro Lys 
            180                 185                 190         


Asp Tyr Thr Gly Glu Glu Val Asn Ala Lys Asn Phe Leu Ala Ala Ile 
        195                 200                 205             


Leu Gly Asn Lys Ser Ala Ile Thr Gly Gly Ser Gly Lys Val Val Asp 
    210                 215                 220                 


Ser Gly Pro Asn Asp His Ile Phe Ile Tyr Tyr Thr Asp His Gly Ala 
225                 230                 235                 240 


Ala Gly Val Ile Gly Met Pro Ser Lys Pro Tyr Leu Tyr Ala Asp Glu 
                245                 250                 255     


Leu Asn Asp Ala Leu Lys Lys Lys His Ala Ser Gly Thr Tyr Lys Ser 
            260                 265                 270         


Leu Val Phe Tyr Leu Glu Ala Cys Glu Ser Gly Ser Met Phe Glu Gly 
        275                 280                 285             


Ile Leu Pro Glu Asp Leu Asn Ile Tyr Ala Leu Thr Ser Thr Asn Thr 
    290                 295                 300                 


Thr Glu Ser Ser Trp Cys Tyr Tyr Cys Pro Ala Gln Glu Asn Pro Pro 
305                 310                 315                 320 


Pro Pro Glu Tyr Asn Val Cys Leu Gly Asp Leu Phe Ser Val Ala Trp 
                325                 330                 335     


Leu Glu Asp Ser Asp Val Gln Asn Ser Trp Tyr Glu Thr Leu Asn Gln 
            340                 345                 350         


Gln Tyr His His Val Asp Lys Arg Ile Ser His Ala Ser His Ala Thr 
        355                 360                 365             


Gln Tyr Gly Asn Leu Lys Leu Gly Glu Glu Gly Leu Phe Val Tyr Met 
    370                 375                 380                 


Gly Ser Asn Pro Ala Asn Asp Asn Tyr Thr Ser Leu Asp Gly Asn Ala 
385                 390                 395                 400 


Leu Thr Pro Ser Ser Ile Val Val Asn Gln Arg Asp Ala Asp Leu Leu 
                405                 410                 415     


His Leu Trp Glu Lys Phe Arg Lys Ala Pro Glu Gly Ser Ala Arg Lys 
            420                 425                 430         


Glu Glu Ala Gln Thr Gln Ile Phe Lys Ala Met Ser His Arg Val His 
        435                 440                 445             


Ile Asp Ser Ser Ile Lys Leu Ile Gly Lys Leu Leu Phe Gly Ile Glu 
    450                 455                 460                 


Lys Cys Thr Glu Ile Leu Asn Ala Val Arg Pro Ala Gly Gln Pro Leu 
465                 470                 475                 480 


Val Asp Asp Trp Ala Cys Leu Arg Ser Leu Val Gly Thr Phe Glu Thr 
                485                 490                 495     


His Cys Gly Ser Leu Ser Glu Tyr Gly Met Arg His Thr Arg Thr Ile 
            500                 505                 510         


Ala Asn Ile Cys Asn Ala Gly Ile Ser Glu Glu Gln Met Ala Glu Ala 
        515                 520                 525             


Ala Ser Gln Ala Cys Ala Ser Ile Pro 
    530                 535         


<210>  13
<211>  10
<212>  PRT
<213>  artificial sequence

<220>
<223>  artificial sequence

<400>  13

Met Gly Met Ala His His His His His His 
1               5                   10  


<210>  14
<211>  76
<212>  PRT
<213>  artificial sequence

<220>
<223>  artificial sequence

<400>  14

Met Gln Ile Phe Val Lys Thr Leu Thr Gly Lys Thr Ile Thr Leu Glu 
1               5                   10                  15      


Val Glu Pro Ser Asp Thr Ile Glu Asn Val Lys Ala Lys Ile Gln Asp 
            20                  25                  30          


Lys Glu Gly Ile Pro Pro Asp Gln Gln Arg Leu Ile Phe Ala Gly Lys 
        35                  40                  45              


Gln Leu Glu Asp Gly Arg Thr Leu Ser Asp Tyr Asn Ile Gln Lys Glu 
    50                  55                  60                  


Ser Thr Leu His Leu Val Leu Arg Leu Arg Gly Gly 
65                  70                  75      


<210>  15
<211>  450
<212>  PRT
<213>  Ovaloparmena affinis

<400>  15

Ala Arg Gly Asp Tyr Leu His Leu Pro Ser Glu Val Ser Arg Phe Phe 
1               5                   10                  15      


Arg Pro Gln Glu Thr Asn Asp Asp His Gly Glu Asp Ser Val Gly Thr 
            20                  25                  30          


Arg Trp Ala Val Leu Ile Ala Gly Ser Lys Gly Tyr Ala Asn Tyr Arg 
        35                  40                  45              


His Gln Ala Gly Val Cys His Ala Tyr Gln Ile Leu Lys Arg Gly Gly 
    50                  55                  60                  


Leu Lys Asp Glu Asn Ile Val Val Phe Met Tyr Asp Asp Ile Ala Tyr 
65                  70                  75                  80  


Asn Glu Ser Asn Pro Arg Pro Gly Val Ile Ile Asn Ser Pro His Gly 
                85                  90                  95      


Ser Asp Val Tyr Ala Gly Val Pro Lys Asp Tyr Thr Gly Glu Glu Val 
            100                 105                 110         


Asn Ala Lys Asn Phe Leu Ala Ala Ile Leu Gly Asn Lys Ser Ala Ile 
        115                 120                 125             


Thr Gly Gly Ser Gly Lys Val Val Asp Ser Gly Pro Asn Asp His Ile 
    130                 135                 140                 


Phe Ile Tyr Tyr Thr Asp His Gly Ala Ala Gly Val Ile Gly Met Pro 
145                 150                 155                 160 


Ser Lys Pro Tyr Leu Tyr Ala Asp Glu Leu Asn Asp Ala Leu Lys Lys 
                165                 170                 175     


Lys His Ala Ser Gly Thr Tyr Lys Ser Leu Val Phe Tyr Leu Glu Ala 
            180                 185                 190         


Cys Glu Ser Gly Ser Met Phe Glu Gly Ile Leu Pro Glu Asp Leu Asn 
        195                 200                 205             


Ile Tyr Ala Leu Thr Ser Thr Asn Thr Thr Glu Ser Ser Trp Cys Tyr 
    210                 215                 220                 


Tyr Cys Pro Ala Gln Glu Asn Pro Pro Pro Pro Glu Tyr Asn Val Cys 
225                 230                 235                 240 


Leu Gly Asp Leu Phe Ser Val Ala Trp Leu Glu Asp Ser Asp Val Gln 
                245                 250                 255     


Asn Ser Trp Tyr Glu Thr Leu Asn Gln Gln Tyr His His Val Asp Lys 
            260                 265                 270         


Arg Ile Ser His Ala Ser His Ala Thr Gln Tyr Gly Asn Leu Lys Leu 
        275                 280                 285             


Gly Glu Glu Gly Leu Phe Val Tyr Met Gly Ser Asn Pro Ala Asn Asp 
    290                 295                 300                 


Asn Tyr Thr Ser Leu Asp Gly Asn Ala Leu Thr Pro Ser Ser Ile Val 
305                 310                 315                 320 


Val Asn Gln Arg Asp Ala Asp Leu Leu His Leu Trp Glu Lys Phe Arg 
                325                 330                 335     


Lys Ala Pro Glu Gly Ser Ala Arg Lys Glu Glu Ala Gln Thr Gln Ile 
            340                 345                 350         


Phe Lys Ala Met Ser His Arg Val His Ile Asp Ser Ser Ile Lys Leu 
        355                 360                 365             


Ile Gly Lys Leu Leu Phe Gly Ile Glu Lys Cys Thr Glu Ile Leu Asn 
    370                 375                 380                 


Ala Val Arg Pro Ala Gly Gln Pro Leu Val Asp Asp Trp Ala Cys Leu 
385                 390                 395                 400 


Arg Ser Leu Val Gly Thr Phe Glu Thr His Cys Gly Ser Leu Ser Glu 
                405                 410                 415     


Tyr Gly Met Arg His Thr Arg Thr Ile Ala Asn Ile Cys Asn Ala Gly 
            420                 425                 430         


Ile Ser Glu Glu Gln Met Ala Glu Ala Ala Ser Gln Ala Cys Ala Ser 
        435                 440                 445             


Ile Pro 
    450 


<210>  16
<211>  38
<212>  DNA
<213>  artificial sequence

<220>
<223>  primer used for mutagenesis

<400>  16
caccaccgaa agcagctggg cctactattg cccggcgc                               38


<210>  17
<211>  14
<212>  PRT
<213>  artificial sequence

<220>
<223>  Peptide template used for characterizing recognition sequence 
       specificity of Quicklase


<220>
<221>  MISC_FEATURE
<222>  (1)..(1)
<223>  Xaa is any amino acid. In each screen, only one of the residue is
       modified. The default amino acid at Xaa1 position is Gly, at Xaa2
       position is Leu, and at Xaa3 position is Gly, and at the Xaa4 
       position is Leu, in this screen.

<220>
<221>  MISC_FEATURE
<222>  (2)..(2)
<223>  Xaa is any amino acid. In each screen, only one of the residue is
       modified. The default amino acid at Xaa1 position is Gly, at Xaa2
       position is Leu, and at Xaa3 position is Gly, and at the Xaa4 
       position is Leu, in this screen.

<220>
<221>  MISC_FEATURE
<222>  (13)..(13)
<223>  Xaa is any amino acid. In each screen, only one of the residue is
       modified. The default amino acid at Xaa1 position is Gly, at Xaa2
       position is Leu, and at Xaa3 position is Gly, and at the Xaa4 
       position is Leu, in this screen.

<220>
<221>  MISC_FEATURE
<222>  (14)..(14)
<223>  Xaa is any amino acid. In each screen, only one of the residue is
       modified. The default amino acid at Xaa1 position is Gly, at Xaa2
       position is Leu, and at Xaa3 position is Gly, and at the Xaa4 
       position is Leu, in this screen.

<400>  17

Xaa Xaa Tyr Arg Arg Gly Arg Leu Tyr Arg Arg Asn Xaa Xaa 
1               5                   10                  


