                         SEQUENCE LISTING

<110>  Bar Ilan University
 
<120>  WASP-PROTECTING SMALL MOLECULES, COMPOSITIONS, METHODS AND USES 
       THEREOF IN THE TREATMENT OF INNATE AND ACQUIRED IMMUNE-RELATED 
       DISORDERS OR CONDITIONS

<130>  2509126

<150>  IL 248028
<151>  2016-09-25

<160>  18    

<170>  PatentIn version 3.5

<210>  1
<211>  502
<212>  PRT
<213>  Homo sapiens

<400>  1

Met Ser Gly Gly Pro Met Gly Gly Arg Pro Gly Gly Arg Gly Ala Pro 
1               5                   10                  15      


Ala Val Gln Gln Asn Ile Pro Ser Thr Leu Leu Gln Asp His Glu Asn 
            20                  25                  30          


Gln Gln Leu Phe Glu Met Leu Gly Arg Lys Cys Leu Thr Leu Ala Thr 
        35                  40                  45              


Ala Val Val Gln Leu Tyr Leu Ala Leu Pro Pro Gly Ala Glu His Trp 
    50                  55                  60                  


Thr Lys Glu His Cys Gly Ala Val Cys Phe Val Lys Asp Asn Pro Gln 
65                  70                  75                  80  


Lys Ser Tyr Phe Ile Arg Leu Tyr Gly Leu Gln Ala Gly Arg Leu Leu 
                85                  90                  95      


Trp Glu Gln Glu Leu Tyr Ser Gln Leu Val Tyr Ser Thr Pro Thr Pro 
            100                 105                 110         


Phe Phe His Thr Phe Ala Gly Asp Asp Cys Gln Ala Gly Leu Asn Phe 
        115                 120                 125             


Ala Asp Glu Asp Glu Ala Gln Ala Phe Arg Ala Leu Val Gln Glu Lys 
    130                 135                 140                 


Ile Gln Lys Arg Asn Gln Arg Gln Ser Gly Asp Arg Arg Gln Leu Pro 
145                 150                 155                 160 


Pro Pro Pro Thr Pro Ala Asn Glu Glu Arg Arg Gly Gly Leu Pro Pro 
                165                 170                 175     


Leu Pro Leu His Pro Gly Gly Asp Gln Gly Gly Pro Pro Val Gly Pro 
            180                 185                 190         


Leu Ser Leu Gly Leu Ala Thr Val Asp Ile Gln Asn Pro Asp Ile Thr 
        195                 200                 205             


Ser Ser Arg Tyr Arg Gly Leu Pro Ala Pro Gly Pro Ser Pro Ala Asp 
    210                 215                 220                 


Lys Lys Arg Ser Gly Arg Lys Lys Ile Ser Lys Ala Asp Ile Gly Ala 
225                 230                 235                 240 


Pro Ser Gly Phe Lys His Val Ser His Val Gly Trp Asp Pro Gln Asn 
                245                 250                 255     


Gly Phe Asp Val Asn Asn Leu Asp Pro Asp Leu Arg Ser Leu Phe Ser 
            260                 265                 270         


Arg Ala Gly Ile Ser Glu Ala Gln Leu Thr Asp Ala Glu Thr Ser Lys 
        275                 280                 285             


Leu Ile Tyr Asp Phe Ile Glu Asp Gln Gly Gly Leu Glu Ala Val Arg 
    290                 295                 300                 


Gln Glu Met Arg Arg Gln Glu Pro Leu Pro Pro Pro Pro Pro Pro Ser 
305                 310                 315                 320 


Arg Gly Gly Asn Gln Leu Pro Arg Pro Pro Ile Val Gly Gly Asn Lys 
                325                 330                 335     


Gly Arg Ser Gly Pro Leu Pro Pro Val Pro Leu Gly Ile Ala Pro Pro 
            340                 345                 350         


Pro Pro Thr Pro Arg Gly Pro Pro Pro Pro Gly Arg Gly Gly Pro Pro 
        355                 360                 365             


Pro Pro Pro Pro Pro Ala Thr Gly Arg Ser Gly Pro Leu Pro Pro Pro 
    370                 375                 380                 


Pro Pro Gly Ala Gly Gly Pro Pro Met Pro Pro Pro Pro Pro Pro Pro 
385                 390                 395                 400 


Pro Pro Pro Pro Ser Ser Gly Asn Gly Pro Ala Pro Pro Pro Leu Pro 
                405                 410                 415     


Pro Ala Leu Val Pro Ala Gly Gly Leu Ala Pro Gly Gly Gly Arg Gly 
            420                 425                 430         


Ala Leu Leu Asp Gln Ile Arg Gln Gly Ile Gln Leu Asn Lys Thr Pro 
        435                 440                 445             


Gly Ala Pro Glu Ser Ser Ala Leu Gln Pro Pro Pro Gln Ser Ser Glu 
    450                 455                 460                 


Gly Leu Val Gly Ala Leu Met His Val Met Gln Lys Arg Ser Arg Ala 
465                 470                 475                 480 


Ile His Ser Ser Asp Glu Gly Glu Asp Gln Ala Gly Asp Glu Asp Glu 
                485                 490                 495     


Asp Asp Glu Trp Asp Asp 
            500         


<210>  2
<211>  1509
<212>  DNA
<213>  Homo sapiens

<400>  2
atgagtgggg gcccaatggg aggaaggccc gggggccgag gagcaccagc ggttcagcag       60

aacataccct ccaccctcct ccaggaccac gagaaccagc aactctttga gatgcttgga      120

cgaaaatgct tgacgctggc cactgcagtt gttcagctgt acctggcgct gccccctgga      180

gctgagcact ggaccaagga gcattgtggg gctgtgtgct tcgtgaagga taacccccag      240

aagtcctact tcatccgcct ttacggcctt caggctggtc ggctgctctg ggaacaggag      300

ctgtactcac agcttgtcta ctccaccccc acccccttct tccacacctt cgctggagat      360

gactgccaag cggggctgaa ctttgcagac gaggacgagg cccaggcctt ccgggccctc      420

gtgcaggaga agatacaaaa aaggaatcag aggcaaagtg gagacagacg ccagctaccc      480

ccaccaccaa caccagccaa tgaagagaga agaggagggc tcccacccct gcccctgcat      540

ccaggtggag accaaggagg ccctccagtg ggtccgctct ccctggggct ggcgacagtg      600

gacatccaga accctgacat cacgagttca cgataccgtg ggctcccagc acctggacct      660

agcccagctg ataagaaacg ctcagggagg aagaagatca gcaaagctga tattggtgca      720

cccagtggat tcaagcatgt cagccacgtg gggtgggacc cccagaatgg atttgacgtg      780

aacaacctcg acccagatct gcggagtctg ttctccaggg caggaatcag cgaggcccag      840

ctcaccgacg ccgagacctc taaacttatc tacgacttca ttgaggacca gggtgggctg      900

gaggctgtgc ggcaggagat gaggcgccag gagccacttc cgccgccccc accgccatct      960

cgaggaggga accagctccc ccggccccct attgtggggg gtaacaaggg tcgttctggt     1020

ccactgcccc ctgtaccttt ggggattgcc ccacccccac caacaccccg gggaccccca     1080

cccccaggcc gagggggccc tccaccacca ccccctccag ctactggacg ttctggacca     1140

ctgccccctc caccccctgg agctggtggg ccacccatgc caccaccacc gccaccaccg     1200

ccaccgccgc ccagctccgg gaatggacca gcccctcccc cactccctcc tgctctggtg     1260

cctgccgggg gcctggcccc tggtgggggt cggggagcgc ttttggatca aatccggcag     1320

ggaattcagc tgaacaagac ccctggggcc ccagagagct cagcgctgca gccaccacct     1380

cagagctcag agggactggt gggggccctg atgcacgtga tgcagaagag aagcagagcc     1440

atccactcct ccgacgaagg ggaggaccag gctggcgatg aagatgaaga tgatgaatgg     1500

gatgactag                                                             1509


<210>  3
<211>  25
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  HUMAN PKC theta RNA SEQUENCE

<400>  3
gagucuccgu uggaugaggu ggaua                                             25


<210>  4
<211>  25
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  HUMAN PKC theta RNA SEQUENCE

<400>  4
gcauccguuu cugacgcaca uguuu                                             25


<210>  5
<211>  25
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  HUMAN PKC theta RNA SEQUENCE

<400>  5
ccggccgaaa gugaaaucac cauuu                                             25


<210>  6
<211>  19
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  NONSPECIFIC siRNA

<400>  6
uagcgacuaa acacaucaa                                                    19


<210>  7
<211>  19
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  NONSPECIFIC siRNA

<400>  7
uaaggcuaug aagagauac                                                    19


<210>  8
<211>  19
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  NONSPECIFIC siRNA

<400>  8
auguauuggc cuguauuag                                                    19


<210>  9
<211>  19
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  NONSPECIFIC siRNA

<400>  9
augaacguga auugcucaa                                                    19


<210>  10
<211>  19
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  NONSPECIFIC siRNA

<400>  10
ugguuuacau gucgacuaa                                                    19


<210>  11
<211>  26
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  RNA guide

<400>  11
aaactcgctg gagatgtaag tggatc                                            26


<210>  12
<211>  25
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  RNA guide

<400>  12
caccgatcac ttacatctcc agcga                                             25


<210>  13
<211>  502
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Human WASp mutant R86C

<400>  13

Met Ser Gly Gly Pro Met Gly Gly Arg Pro Gly Gly Arg Gly Ala Pro 
1               5                   10                  15      


Ala Val Gln Gln Asn Ile Pro Ser Thr Leu Leu Gln Asp His Glu Asn 
            20                  25                  30          


Gln Gln Leu Phe Glu Met Leu Gly Arg Lys Cys Leu Thr Leu Ala Thr 
        35                  40                  45              


Ala Val Val Gln Leu Tyr Leu Ala Leu Pro Pro Gly Ala Glu His Trp 
    50                  55                  60                  


Thr Lys Glu His Cys Gly Ala Val Cys Phe Val Lys Asp Asn Pro Gln 
65                  70                  75                  80  


Lys Ser Tyr Phe Ile Cys Leu Tyr Gly Leu Gln Ala Gly Arg Leu Leu 
                85                  90                  95      


Trp Glu Gln Glu Leu Tyr Ser Gln Leu Val Tyr Ser Thr Pro Thr Pro 
            100                 105                 110         


Phe Phe His Thr Phe Ala Gly Asp Asp Cys Gln Ala Gly Leu Asn Phe 
        115                 120                 125             


Ala Asp Glu Asp Glu Ala Gln Ala Phe Arg Ala Leu Val Gln Glu Lys 
    130                 135                 140                 


Ile Gln Lys Arg Asn Gln Arg Gln Ser Gly Asp Arg Arg Gln Leu Pro 
145                 150                 155                 160 


Pro Pro Pro Thr Pro Ala Asn Glu Glu Arg Arg Gly Gly Leu Pro Pro 
                165                 170                 175     


Leu Pro Leu His Pro Gly Gly Asp Gln Gly Gly Pro Pro Val Gly Pro 
            180                 185                 190         


Leu Ser Leu Gly Leu Ala Thr Val Asp Ile Gln Asn Pro Asp Ile Thr 
        195                 200                 205             


Ser Ser Arg Tyr Arg Gly Leu Pro Ala Pro Gly Pro Ser Pro Ala Asp 
    210                 215                 220                 


Lys Lys Arg Ser Gly Arg Lys Lys Ile Ser Lys Ala Asp Ile Gly Ala 
225                 230                 235                 240 


Pro Ser Gly Phe Lys His Val Ser His Val Gly Trp Asp Pro Gln Asn 
                245                 250                 255     


Gly Phe Asp Val Asn Asn Leu Asp Pro Asp Leu Arg Ser Leu Phe Ser 
            260                 265                 270         


Arg Ala Gly Ile Ser Glu Ala Gln Leu Thr Asp Ala Glu Thr Ser Lys 
        275                 280                 285             


Leu Ile Tyr Asp Phe Ile Glu Asp Gln Gly Gly Leu Glu Ala Val Arg 
    290                 295                 300                 


Gln Glu Met Arg Arg Gln Glu Pro Leu Pro Pro Pro Pro Pro Pro Ser 
305                 310                 315                 320 


Arg Gly Gly Asn Gln Leu Pro Arg Pro Pro Ile Val Gly Gly Asn Lys 
                325                 330                 335     


Gly Arg Ser Gly Pro Leu Pro Pro Val Pro Leu Gly Ile Ala Pro Pro 
            340                 345                 350         


Pro Pro Thr Pro Arg Gly Pro Pro Pro Pro Gly Arg Gly Gly Pro Pro 
        355                 360                 365             


Pro Pro Pro Pro Pro Ala Thr Gly Arg Ser Gly Pro Leu Pro Pro Pro 
    370                 375                 380                 


Pro Pro Gly Ala Gly Gly Pro Pro Met Pro Pro Pro Pro Pro Pro Pro 
385                 390                 395                 400 


Pro Pro Pro Pro Ser Ser Gly Asn Gly Pro Ala Pro Pro Pro Leu Pro 
                405                 410                 415     


Pro Ala Leu Val Pro Ala Gly Gly Leu Ala Pro Gly Gly Gly Arg Gly 
            420                 425                 430         


Ala Leu Leu Asp Gln Ile Arg Gln Gly Ile Gln Leu Asn Lys Thr Pro 
        435                 440                 445             


Gly Ala Pro Glu Ser Ser Ala Leu Gln Pro Pro Pro Gln Ser Ser Glu 
    450                 455                 460                 


Gly Leu Val Gly Ala Leu Met His Val Met Gln Lys Arg Ser Arg Ala 
465                 470                 475                 480 


Ile His Ser Ser Asp Glu Gly Glu Asp Gln Ala Gly Asp Glu Asp Glu 
                485                 490                 495     


Asp Asp Glu Trp Asp Asp 
            500         


<210>  14
<211>  1509
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Human WASp mutant R86C

<400>  14
atgagtgggg gcccaatggg aggaaggccc gggggccgag gagcaccagc ggttcagcag       60

aacataccct ccaccctcct ccaggaccac gagaaccagc aactctttga gatgcttgga      120

cgaaaatgct tgacgctggc cactgcagtt gttcagctgt acctggcgct gccccctgga      180

gctgagcact ggaccaagga gcattgtggg gctgtgtgct tcgtgaagga taacccccag      240

aagtcctact tcatctgcct ttacggcctt caggctggtc ggctgctctg ggaacaggag      300

ctgtactcac agcttgtcta ctccaccccc acccccttct tccacacctt cgctggagat      360

gactgccaag cggggctgaa ctttgcagac gaggacgagg cccaggcctt ccgggccctc      420

gtgcaggaga agatacaaaa aaggaatcag aggcaaagtg gagacagacg ccagctaccc      480

ccaccaccaa caccagccaa tgaagagaga agaggagggc tcccacccct gcccctgcat      540

ccaggtggag accaaggagg ccctccagtg ggtccgctct ccctggggct ggcgacagtg      600

gacatccaga accctgacat cacgagttca cgataccgtg ggctcccagc acctggacct      660

agcccagctg ataagaaacg ctcagggagg aagaagatca gcaaagctga tattggtgca      720

cccagtggat tcaagcatgt cagccacgtg gggtgggacc cccagaatgg atttgacgtg      780

aacaacctcg acccagatct gcggagtctg ttctccaggg caggaatcag cgaggcccag      840

ctcaccgacg ccgagacctc taaacttatc tacgacttca ttgaggacca gggtgggctg      900

gaggctgtgc ggcaggagat gaggcgccag gagccacttc cgccgccccc accgccatct      960

cgaggaggga accagctccc ccggccccct attgtggggg gtaacaaggg tcgttctggt     1020

ccactgcccc ctgtaccttt ggggattgcc ccacccccac caacaccccg gggaccccca     1080

cccccaggcc gagggggccc tccaccacca ccccctccag ctactggacg ttctggacca     1140

ctgccccctc caccccctgg agctggtggg ccacccatgc caccaccacc gccaccaccg     1200

ccaccgccgc ccagctccgg gaatggacca gcccctcccc cactccctcc tgctctggtg     1260

cctgccgggg gcctggcccc tggtgggggt cggggagcgc ttttggatca aatccggcag     1320

ggaattcagc tgaacaagac ccctggggcc ccagagagct cagcgctgca gccaccacct     1380

cagagctcag agggactggt gggggccctg atgcacgtga tgcagaagag aagcagagcc     1440

atccactcct ccgacgaagg ggaggaccag gctggcgatg aagatgaaga tgatgaatgg     1500

gatgactag                                                             1509


<210>  15
<211>  502
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Human WASp mutant Y107C

<400>  15

Met Ser Gly Gly Pro Met Gly Gly Arg Pro Gly Gly Arg Gly Ala Pro 
1               5                   10                  15      


Ala Val Gln Gln Asn Ile Pro Ser Thr Leu Leu Gln Asp His Glu Asn 
            20                  25                  30          


Gln Gln Leu Phe Glu Met Leu Gly Arg Lys Cys Leu Thr Leu Ala Thr 
        35                  40                  45              


Ala Val Val Gln Leu Tyr Leu Ala Leu Pro Pro Gly Ala Glu His Trp 
    50                  55                  60                  


Thr Lys Glu His Cys Gly Ala Val Cys Phe Val Lys Asp Asn Pro Gln 
65                  70                  75                  80  


Lys Ser Tyr Phe Ile Arg Leu Tyr Gly Leu Gln Ala Gly Arg Leu Leu 
                85                  90                  95      


Trp Glu Gln Glu Leu Tyr Ser Gln Leu Val Cys Ser Thr Pro Thr Pro 
            100                 105                 110         


Phe Phe His Thr Phe Ala Gly Asp Asp Cys Gln Ala Gly Leu Asn Phe 
        115                 120                 125             


Ala Asp Glu Asp Glu Ala Gln Ala Phe Arg Ala Leu Val Gln Glu Lys 
    130                 135                 140                 


Ile Gln Lys Arg Asn Gln Arg Gln Ser Gly Asp Arg Arg Gln Leu Pro 
145                 150                 155                 160 


Pro Pro Pro Thr Pro Ala Asn Glu Glu Arg Arg Gly Gly Leu Pro Pro 
                165                 170                 175     


Leu Pro Leu His Pro Gly Gly Asp Gln Gly Gly Pro Pro Val Gly Pro 
            180                 185                 190         


Leu Ser Leu Gly Leu Ala Thr Val Asp Ile Gln Asn Pro Asp Ile Thr 
        195                 200                 205             


Ser Ser Arg Tyr Arg Gly Leu Pro Ala Pro Gly Pro Ser Pro Ala Asp 
    210                 215                 220                 


Lys Lys Arg Ser Gly Arg Lys Lys Ile Ser Lys Ala Asp Ile Gly Ala 
225                 230                 235                 240 


Pro Ser Gly Phe Lys His Val Ser His Val Gly Trp Asp Pro Gln Asn 
                245                 250                 255     


Gly Phe Asp Val Asn Asn Leu Asp Pro Asp Leu Arg Ser Leu Phe Ser 
            260                 265                 270         


Arg Ala Gly Ile Ser Glu Ala Gln Leu Thr Asp Ala Glu Thr Ser Lys 
        275                 280                 285             


Leu Ile Tyr Asp Phe Ile Glu Asp Gln Gly Gly Leu Glu Ala Val Arg 
    290                 295                 300                 


Gln Glu Met Arg Arg Gln Glu Pro Leu Pro Pro Pro Pro Pro Pro Ser 
305                 310                 315                 320 


Arg Gly Gly Asn Gln Leu Pro Arg Pro Pro Ile Val Gly Gly Asn Lys 
                325                 330                 335     


Gly Arg Ser Gly Pro Leu Pro Pro Val Pro Leu Gly Ile Ala Pro Pro 
            340                 345                 350         


Pro Pro Thr Pro Arg Gly Pro Pro Pro Pro Gly Arg Gly Gly Pro Pro 
        355                 360                 365             


Pro Pro Pro Pro Pro Ala Thr Gly Arg Ser Gly Pro Leu Pro Pro Pro 
    370                 375                 380                 


Pro Pro Gly Ala Gly Gly Pro Pro Met Pro Pro Pro Pro Pro Pro Pro 
385                 390                 395                 400 


Pro Pro Pro Pro Ser Ser Gly Asn Gly Pro Ala Pro Pro Pro Leu Pro 
                405                 410                 415     


Pro Ala Leu Val Pro Ala Gly Gly Leu Ala Pro Gly Gly Gly Arg Gly 
            420                 425                 430         


Ala Leu Leu Asp Gln Ile Arg Gln Gly Ile Gln Leu Asn Lys Thr Pro 
        435                 440                 445             


Gly Ala Pro Glu Ser Ser Ala Leu Gln Pro Pro Pro Gln Ser Ser Glu 
    450                 455                 460                 


Gly Leu Val Gly Ala Leu Met His Val Met Gln Lys Arg Ser Arg Ala 
465                 470                 475                 480 


Ile His Ser Ser Asp Glu Gly Glu Asp Gln Ala Gly Asp Glu Asp Glu 
                485                 490                 495     


Asp Asp Glu Trp Asp Asp 
            500         


<210>  16
<211>  1509
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Human WASp mutant Y107C

<400>  16
atgagtgggg gcccaatggg aggaaggccc gggggccgag gagcaccagc ggttcagcag       60

aacataccct ccaccctcct ccaggaccac gagaaccagc aactctttga gatgcttgga      120

cgaaaatgct tgacgctggc cactgcagtt gttcagctgt acctggcgct gccccctgga      180

gctgagcact ggaccaagga gcattgtggg gctgtgtgct tcgtgaagga taacccccag      240

aagtcctact tcatccgcct ttacggcctt caggctggtc ggctgctctg ggaacaggag      300

ctgtactcac agcttgtctg ctccaccccc acccccttct tccacacctt cgctggagat      360

gactgccaag cggggctgaa ctttgcagac gaggacgagg cccaggcctt ccgggccctc      420

gtgcaggaga agatacaaaa aaggaatcag aggcaaagtg gagacagacg ccagctaccc      480

ccaccaccaa caccagccaa tgaagagaga agaggagggc tcccacccct gcccctgcat      540

ccaggtggag accaaggagg ccctccagtg ggtccgctct ccctggggct ggcgacagtg      600

gacatccaga accctgacat cacgagttca cgataccgtg ggctcccagc acctggacct      660

agcccagctg ataagaaacg ctcagggagg aagaagatca gcaaagctga tattggtgca      720

cccagtggat tcaagcatgt cagccacgtg gggtgggacc cccagaatgg atttgacgtg      780

aacaacctcg acccagatct gcggagtctg ttctccaggg caggaatcag cgaggcccag      840

ctcaccgacg ccgagacctc taaacttatc tacgacttca ttgaggacca gggtgggctg      900

gaggctgtgc ggcaggagat gaggcgccag gagccacttc cgccgccccc accgccatct      960

cgaggaggga accagctccc ccggccccct attgtggggg gtaacaaggg tcgttctggt     1020

ccactgcccc ctgtaccttt ggggattgcc ccacccccac caacaccccg gggaccccca     1080

cccccaggcc gagggggccc tccaccacca ccccctccag ctactggacg ttctggacca     1140

ctgccccctc caccccctgg agctggtggg ccacccatgc caccaccacc gccaccaccg     1200

ccaccgccgc ccagctccgg gaatggacca gcccctcccc cactccctcc tgctctggtg     1260

cctgccgggg gcctggcccc tggtgggggt cggggagcgc ttttggatca aatccggcag     1320

ggaattcagc tgaacaagac ccctggggcc ccagagagct cagcgctgca gccaccacct     1380

cagagctcag agggactggt gggggccctg atgcacgtga tgcagaagag aagcagagcc     1440

atccactcct ccgacgaagg ggaggaccag gctggcgatg aagatgaaga tgatgaatgg     1500

gatgactag                                                             1509


<210>  17
<211>  499
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Human WASp mutant A134T

<400>  17

Met Ser Gly Gly Pro Met Gly Gly Arg Pro Gly Gly Arg Gly Ala Pro 
1               5                   10                  15      


Ala Val Gln Gln Asn Ile Pro Ser Thr Leu Leu Gln Asp His Glu Asn 
            20                  25                  30          


Gln Gln Leu Phe Glu Met Leu Gly Arg Lys Cys Leu Thr Leu Ala Thr 
        35                  40                  45              


Ala Val Val Gln Leu Tyr Leu Ala Leu Pro Pro Gly Ala Glu His Trp 
    50                  55                  60                  


Thr Lys Glu His Cys Gly Ala Val Cys Phe Val Lys Asp Asn Pro Gln 
65                  70                  75                  80  


Lys Ser Tyr Phe Ile Arg Leu Tyr Gly Leu Gln Ala Gly Arg Leu Leu 
                85                  90                  95      


Trp Glu Gln Glu Leu Tyr Ser Gln Leu Val Tyr Ser Thr Pro Thr Pro 
            100                 105                 110         


Phe Phe His Thr Phe Ala Gly Asp Asp Cys Gln Ala Gly Leu Asn Phe 
        115                 120                 125             


Ala Asp Glu Asp Glu Thr Gln Arg Ala Leu Val Gln Glu Lys Ile Gln 
    130                 135                 140                 


Lys Arg Asn Gln Arg Gln Ser Gly Asp Arg Arg Gln Leu Pro Pro Pro 
145                 150                 155                 160 


Pro Thr Pro Ala Asn Glu Glu Arg Arg Gly Gly Leu Pro Pro Leu Pro 
                165                 170                 175     


Leu His Pro Gly Gly Asp Gln Gly Gly Pro Pro Val Gly Pro Leu Ser 
            180                 185                 190         


Leu Gly Leu Ala Thr Val Asp Ile Gln Asn Pro Asp Ile Thr Ser Ser 
        195                 200                 205             


Arg Tyr Arg Gly Leu Pro Ala Pro Gly Pro Ser Pro Ala Asp Lys Lys 
    210                 215                 220                 


Arg Ser Gly Arg Lys Lys Ile Ser Lys Ala Asp Ile Gly Ala Pro Ser 
225                 230                 235                 240 


Gly Phe Lys His Val Ser His Val Gly Trp Asp Pro Gln Asn Gly Phe 
                245                 250                 255     


Asp Val Asn Asn Leu Asp Pro Asp Leu Arg Ser Leu Phe Ser Arg Ala 
            260                 265                 270         


Gly Ile Ser Glu Ala Gln Leu Thr Asp Ala Glu Thr Ser Lys Leu Ile 
        275                 280                 285             


Tyr Asp Phe Ile Glu Asp Gln Gly Gly Leu Glu Ala Val Arg Gln Glu 
    290                 295                 300                 


Met Arg Arg Gln Glu Pro Leu Pro Pro Pro Pro Pro Pro Ser Arg Gly 
305                 310                 315                 320 


Gly Asn Gln Leu Pro Arg Pro Pro Ile Val Gly Gly Asn Lys Gly Arg 
                325                 330                 335     


Ser Gly Pro Leu Pro Pro Val Pro Gly Ile Ala Pro Pro Pro Pro Thr 
            340                 345                 350         


Pro Arg Gly Pro Pro Pro Pro Gly Arg Gly Gly Pro Pro Pro Pro Pro 
        355                 360                 365             


Pro Pro Ala Thr Gly Arg Ser Gly Pro Leu Pro Pro Pro Pro Pro Gly 
    370                 375                 380                 


Ala Gly Gly Pro Pro Met Pro Pro Pro Pro Pro Pro Pro Pro Pro Pro 
385                 390                 395                 400 


Pro Ser Ser Gly Asn Gly Pro Ala Pro Pro Pro Leu Pro Pro Ala Leu 
                405                 410                 415     


Val Pro Ala Gly Gly Leu Ala Pro Gly Gly Gly Arg Gly Ala Leu Leu 
            420                 425                 430         


Asp Gln Ile Arg Gln Gly Ile Gln Leu Asn Lys Thr Pro Gly Ala Pro 
        435                 440                 445             


Glu Ser Ser Ala Leu Gln Pro Pro Pro Gln Ser Ser Glu Gly Leu Val 
    450                 455                 460                 


Gly Ala Leu Met His Val Met Gln Lys Arg Ser Arg Ala Ile His Ser 
465                 470                 475                 480 


Ser Asp Glu Gly Glu Asp Gln Ala Gly Asp Glu Asp Glu Asp Asp Glu 
                485                 490                 495     


Trp Asp Asp 
            


<210>  18
<211>  1509
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Human WASp mutant A134T

<400>  18
atgagtgggg gcccaatggg aggaaggccc gggggccgag gagcaccagc ggttcagcag       60

aacataccct ccaccctcct ccaggaccac gagaaccagc aactctttga gatgcttgga      120

cgaaaatgct tgacgctggc cactgcagtt gttcagctgt acctggcgct gccccctgga      180

gctgagcact ggaccaagga gcattgtggg gctgtgtgct tcgtgaagga taacccccag      240

aagtcctact tcatccgcct ttacggcctt caggctggtc ggctgctctg ggaacaggag      300

ctgtactcac agcttgtcta ctccaccccc acccccttct tccacacctt cgctggagat      360

gactgccaag cggggctgaa ctttgcagac gaggacgaga cccaggcctt ccgggccctc      420

gtgcaggaga agatacaaaa aaggaatcag aggcaaagtg gagacagacg ccagctaccc      480

ccaccaccaa caccagccaa tgaagagaga agaggagggc tcccacccct gcccctgcat      540

ccaggtggag accaaggagg ccctccagtg ggtccgctct ccctggggct ggcgacagtg      600

gacatccaga accctgacat cacgagttca cgataccgtg ggctcccagc acctggacct      660

agcccagctg ataagaaacg ctcagggagg aagaagatca gcaaagctga tattggtgca      720

cccagtggat tcaagcatgt cagccacgtg gggtgggacc cccagaatgg atttgacgtg      780

aacaacctcg acccagatct gcggagtctg ttctccaggg caggaatcag cgaggcccag      840

ctcaccgacg ccgagacctc taaacttatc tacgacttca ttgaggacca gggtgggctg      900

gaggctgtgc ggcaggagat gaggcgccag gagccacttc cgccgccccc accgccatct      960

cgaggaggga accagctccc ccggccccct attgtggggg gtaacaaggg tcgttctggt     1020

ccactgcccc ctgtaccttt ggggattgcc ccacccccac caacaccccg gggaccccca     1080

cccccaggcc gagggggccc tccaccacca ccccctccag ctactggacg ttctggacca     1140

ctgccccctc caccccctgg agctggtggg ccacccatgc caccaccacc gccaccaccg     1200

ccaccgccgc ccagctccgg gaatggacca gcccctcccc cactccctcc tgctctggtg     1260

cctgccgggg gcctggcccc tggtgggggt cggggagcgc ttttggatca aatccggcag     1320

ggaattcagc tgaacaagac ccctggggcc ccagagagct cagcgctgca gccaccacct     1380

cagagctcag agggactggt gggggccctg atgcacgtga tgcagaagag aagcagagcc     1440

atccactcct ccgacgaagg ggaggaccag gctggcgatg aagatgaaga tgatgaatgg     1500

gatgactag                                                             1509


