                         SEQUENCE LISTING

<110>  Bayer CropScience NV
 
<120>  Targeted genome optimization in plants

<130>  BCS 16-2019

<160>  6     

<170>  PatentIn version 3.5

<210>  1
<211>  9186
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Cas 9-vector pBAY00201

<400>  1
tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca       60

cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg      120

ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc      180

accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc      240

attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat      300

tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt      360

tttcccagtc acgacgttgt aaaacgacgg ccagtgaatt gggcccggcc ggccgcgatc      420

gcgcggccgc ctgcagtgca gcgtgacccg gtcgtgcccc tctctagaga taatgagcat      480

tgcatgtcta agttataaaa aattaccaca tatttttttt gtcacacttg tttgaagtgc      540

agtttatcta tctttataca tatatttaaa ctttactcta cgaataatat aatctatagt      600

actacaataa tatcagtgtt ttagagaatc atataaatga acagttagac atggtctaaa      660

ggacaattga gtattttgac aacaggactc tacagtttta tctttttagt gtgcatgtgt      720

tctccttttt ttttgcaaat agcttcacct atataatact tcatccattt tattagtaca      780

tccatttagg gtttagggtt aatggttttt atagactaat ttttttagta catctatttt      840

attctatttt agcctctaaa ttaagaaaac taaaactcta ttttagtttt tttatttaat      900

aatttagata taaaatagaa taaaataaag tgactaaaaa ttaaacaaat accctttaag      960

aaattaaaaa aactaaggaa acatttttct tgtttcgagt agataatgcc agcctgttaa     1020

acgccgtcga tcgacgagtc taacggacac caaccagcga accagcagcg tcgcgtcggg     1080

ccaagcgaag cagacggcac ggcatctctg tcgctgcctc tggacccctc tcgagagttc     1140

cgctccaccg ttggacttgc tccgctgtcg gcatccagaa attgcgtggc ggagcggcag     1200

acgtgagccg gcacggcagg cggcctcctc ctcctctcac ggcaccggca gctacggggg     1260

attcctttcc caccgctcct tcgctttccc ttcctcgccc gccgtaataa atagacaccc     1320

cctccacacc ctctttcccc aacctcgtgt tgttcggagc gcacacacac acaaccagat     1380

ctcccccaaa tccacccgtc ggcacctccg cttcaaggta cgccgctcgt cctccccccc     1440

cccccctctc taccttctct agatcggcgt tccggtccat gcttagggcc cggtagttct     1500

acttctgtcc atgtttgtgt tagatccgtg tttgtgttag atccgtgcta ctagcgttcg     1560

tacacggatg cgacctgtac gtcagacacg ttctgattgc taacttgcca gtgtttctct     1620

ttggggaatc ctgggatggc tctagccgtt ccgcagacgg gatcgatttc atgatttttt     1680

ttgtttcgtt gcatagggtt tggtttgccc ttttccttta tttcaatata tgccgtgcac     1740

ttgtttgtcg ggtcatcttt tcatgctttt ttttgtcttg gttgtgatga tgtggtctgg     1800

ttgggcggtc gttctagatc ggagtagaat tctgtttcaa actacctggt ggatttatta     1860

attttggatc tgtatgtgtg tgccatacat attcatagtt acgaattgaa gatgatggat     1920

ggaaatatcg atctaggata ggtatacatg ttgatgcggg ttttactgat gcatatacag     1980

agatgctttt tgttcgcttg gttgtgatga tgtggtgtgg ttgggcggtc gttcattcgt     2040

tctagatcgg agtagaatac tgtttcaaac tacctggtgt atttattaat tttggaactg     2100

tatgtgtgtg tcatacatct tcatagttac gagtttaaga tggatggaaa tatcgatcta     2160

ggataggtat acatgttgat gtgggtttta ctgatgcata tacatgatgg catatgcagc     2220

atctattcat atgctctaac cttgagtacc tatctattat aataaacaag tatgttttat     2280

aattattttg atcttgatat acttggatga tggcatatgc agcagctata tgtggatttt     2340

tttagccctg ccttcatacg ctatttattt gcttggtact gtttcttttg tcgatgctca     2400

ccctgttgtt tggtgttact tctgcagcca tggcccctaa gaagaaacga aaggtaggca     2460

tccacggtgt tccagcggct atggacaaga agtacagcat cgggctggac attggcacga     2520

actctgttgg gtgggcggtt atcactgacg agtacaaggt gccgtcgaag aagttcaagg     2580

tgctaggcaa caccgaccgt cacagcatca agaagaacct catcggcgcc ctgttgtttg     2640

actctgggga aaccgccgag gctactcgac taaagagaac cgcccgtagg cgatacacta     2700

ggcggaagaa caggatctgt tatctccagg agatcttcag caacgagatg gcgaaggtcg     2760

atgactcatt cttccaccgc cttgaggagt ctttcctggt cgaggaggac aaaaagcacg     2820

agcgacaccc gatcttcggg aacatcgttg atgaggtagc ctaccacgag aagtacccta     2880

ctatctacca cctgcgcaag aaactagtgg actccaccga caaggcggac ctccgcctta     2940

tctatctcgc cctcgcccac atgatcaagt ttcgcgggca cttcctcatc gaaggcgatc     3000

tcaatccgga caacagcgac gtggacaagc tattcatcca gctagttcag acctacaacc     3060

agctctttga agagaaccct attaacgcga gcggggtgga cgccaaagcc atcctttccg     3120

ccaggctctc taagagccgg agactcgaga accttatcgc tcagttgccc ggcgagaaga     3180

aaaacggcct tttcggcaac ctgattgccc tgtccttggg cttgacgccg aacttcaaga     3240

gcaacttcga cctagctgag gatgctaaac tgcagctgtc caaggacact tatgatgacg     3300

accttgacaa cctcctggcc caaattgggg atcagtacgc ggacctcttc ctcgctgcca     3360

agaacctctc cgacgcgatt ctgttgtcgg acatcctccg cgtgaacacc gagattacta     3420

aggccccact gtccgcgagc atgatcaaac gctacgacga gcaccatcag gatctaactc     3480

tgctcaaggc gctcgtccgt cagcaactcc ctgagaagta caaggagatc ttcttcgacc     3540

agtccaagaa cggctacgcc ggttacattg atggtggcgc gagccaggag gagttctaca     3600

agttcatcaa gcccatctta gagaagatgg acggcaccga agagcttctc gtgaagctca     3660

acagggagga cctcctaagg aagcagcgca cctttgacaa cgggtctatt cctcatcaga     3720

tccatctcgg cgagctccac gcgattttga gacgccaaga ggacttctac ccgttcttga     3780

aggacaaccg ggagaagatc gagaagatcc tcacgttcag gatcccttat tacgtcggcc     3840

ccctagctag gggtaactcg agattcgcgt ggatgaccag aaagtccgag gaaaccatta     3900

ccccgtggaa ctttgaagag gtggtcgaca agggcgcgag cgcgcaatcg ttcattgagc     3960

ggatgaccaa cttcgacaag aacctaccga acgagaaggt cctgccgaaa cactccctgc     4020

tctacgagta cttcaccgtt tataacgagc tgacgaaggt caagtacgtg actgagggca     4080

tgcgtaaacc agcgttcctc agcggtgagc agaagaaggc tatcgtggac ctgctcttta     4140

agacgaaccg caaggtgacc gtgaagcagc tcaaggagga ttatttcaag aagatcgagt     4200

gcttcgactc agtggagatt agcggcgtgg aggaccgttt caatgcgagc ttaggtacct     4260

accacgacct cctgaagatc atcaaggaca aggacttcct cgacaacgag gaaaacgagg     4320

acatcctgga ggacatcgtg ctcaccctga ccctctttga agacagggag atgatcgagg     4380

aaaggttgaa gacctacgcg cacctattcg acgacaaggt catgaaacag ctcaagcgga     4440

ggagatacac tggctggggc agactttcgc ggaagctcat caatgggatc agggacaagc     4500

agtccggcaa gaccatcctg gacttcttga agagcgacgg cttcgctaat cgcaacttca     4560

tgcagctcat tcatgacgac tccctaacat tcaaggagga catccaaaag gcgcaagtct     4620

cgggtcaggg ggattctttg cacgagcaca tcgcaaacct cgccggttct ccggccatta     4680

aaaagggcat ccttcagacg gtcaaggtgg tcgacgagct cgtgaaggtt atgggccggc     4740

ataagcccga gaacatagtg atcgagatgg ctagggagaa ccagaccacg caaaagggcc     4800

agaagaactc acgtgagcgc atgaagagga tcgaggaagg tatcaaggag ctgggctctc     4860

agattctcaa ggagcatccg gtcgagaaca cccagcttca gaacgagaag ctgtacctct     4920

actacctcca gaatggccgc gacatgtacg ttgatcagga gttagatatt aatcggctga     4980

gcgactacga cgttgaccac atcgtgcccc agtcgttcct caaggatgac tccatcgaca     5040

acaaggtcct gaccagatcg gacaagaacc gtggtaagag cgacaatgtg ccctccgagg     5100

aggtggtcaa aaagatgaag aactattggc ggcagttgct gaacgccaag ctcatcaccc     5160

agcgaaagtt cgataattta acgaaggccg aacgaggggg cctttccgaa ctcgataagg     5220

ccggtttcat caagcgtcag ctggttgaga cgcgacagat cacgaagcat gtggcccaga     5280

ttctggacag ccggatgaac acgaagtacg acgagaacga caaacttatt cgggaggtga     5340

aggtgataac cctcaagtcc aagttagtgt cggacttccg caaggacttt cagttctata     5400

aagtgcgcga gatcaacaat taccaccacg cccacgacgc ctacttgaat gccgtggtcg     5460

gcacggcgct catcaaaaag taccccaagc tggagagcga gttcgtctac ggcgactaca     5520

aggtttatga cgtcaggaag atgattgcga agtcagagca agagatcggg aaggccaccg     5580

ccaagtactt cttctactcg aacatcatga acttctttaa gaccgagatc accctcgcca     5640

atggggagat ccggaaacgt ccccttatcg agacgaacgg cgagactggc gagatagtct     5700

gggacaaagg tcgcgatttc gccaccgttc gaaaggtgct ctcaatgcct caggtcaata     5760

tcgtgaagaa gaccgaggtt cagacggggg gcttcagcaa ggagtccatc ctccctaagc     5820

gcaactctga caagctgatc gcccggaaaa aggattggga cccgaaaaag tacggcggct     5880

tcgattctcc caccgtagct tatagcgtgc tcgtggttgc caaggtggag aagggcaaga     5940

gcaagaaact gaagtcggtc aaggagttac tcggcatcac aatcatggag cgcagctcgt     6000

ttgaaaagaa cccgatcgac ttcctggagg ccaagggcta taaggaggtg aagaaggacc     6060

tcatcattaa gctgccgaag tacagcttgt tcgagctcga gaatggcagg aagaggatgc     6120

tagcttcggc cggcgaactt caaaaaggga acgagctcgc gttgccgtcg aagtacgtga     6180

acttcctcta cttagctagt cactatgaaa agctgaaggg ctcgccggag gacaacgagc     6240

agaagcagct cttcgtcgag caacacaagc actacctcga cgagatcatc gagcagataa     6300

gtgagttcag caagcgcgtg atcctcgctg atgccaacct cgataaggtg ctcagcgcct     6360

acaataagca ccgagacaag ccgattcggg agcaggcgga gaacatcatt catttattta     6420

cgctcactaa cctaggcgct ccggctgcct tcaagtactt cgacaccacg atcgacagga     6480

agcgctacac cagcaccaaa gaagtcctcg acgcgacgct cattcaccag agcatcacag     6540

ggctctacga gactcggatc gacttgtcgc aattgggtgg ggataagagg ccggccgcga     6600

ctaaaaaggc cggccaagcc aagaaaaaga aatgattgct agcacgcgtc gaagcagatc     6660

gttcaaacat ttggcaataa agtttcttaa gattgaatcc tgttgccggt cttgcgatga     6720

ttatcatata atttctgttg aattacgtta agcatgtaat aattaacatg taatgcatga     6780

cgttatttat gagatgggtt tttatgatta gagtcccgca attatacatt taatacgcga     6840

tagaaaacaa aatatagcgc gcaaactagg ataaattatc gcgcgcggtg tcatctatgt     6900

tactagatcc ctgcaggccc gggttaatta aatttaaatg gcgcgccagc ttggcgtaat     6960

catggtcata gctgtttcct gtgtgaaatt gttatccgct cacaattcca cacaacatac     7020

gagccggaag cataaagtgt aaagcctggg gtgcctaatg agtgagctaa ctcacattaa     7080

ttgcgttgcg ctcactgccc gctttccagt cgggaaacct gtcgtgccag ctgcattaat     7140

gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc     7200

tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg     7260

cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag     7320

gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc     7380

gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag     7440

gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga     7500

ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc     7560

atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg     7620

tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt     7680

ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca     7740

gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca     7800

ctagaagaac agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag     7860

ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca     7920

agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg     7980

ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa     8040

aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta     8100

tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag     8160

cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga     8220

tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac     8280

cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc     8340

ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta     8400

gttcgccagt taatagtttg cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac     8460

gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat     8520

gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa     8580

gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg     8640

tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag     8700

aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aatacgggat aataccgcgc     8760

cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct     8820

caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat     8880

cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg     8940

ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc     9000

aatattattg aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta     9060

tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg     9120

tctaagaaac cattattatc atgacattaa cctataaaaa taggcgtatc acgaggccct     9180

ttcgtc                                                                9186


<210>  2
<211>  3318
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  gRNA vector pKVA766

<400>  2
tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca       60

cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg      120

ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc      180

accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc      240

attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat      300

tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt      360

tttcccagtc acgacgttgt aaaacgacgg ccagtgaatt gacgcgtatt gggatggaac      420

cctgcaggaa aaaaaaagca ccgactcggt gccacttttt caagttgata acggactagc      480

cttattttaa cttgctattt ctagctctaa aacttgacag cagccgtgac tgcaagctga      540

ctacagcgcg cgggtttata agctggttcc atcgtctttc ggtttccgat gtggtactaa      600

acatcccccg caacgccacc gcgcaaacca tcggatcccc atccaacgga ctaaagtggg      660

tagcagcgcc acccgcgcgg tacggcgcgc gtgggctgca ccttccgacg cagcccaaat      720

caggatacgg cccattagta tatattctcc tcctcccagc ccacatcaca tgcgtttcac      780

actttcgcct gtaatcgctc aagtagtcaa gtatgagctc tgtctgcacg aactgattgt      840

cactagtgta ccatcaaact actagacgtg acttgtgata atctcagacg cttatggagt      900

gaaacatggt aaaaaaatgc gtttatgtgc ttgatcactt tgatcagcct tgcctccatc      960

gtctgaaaca tgataagaag acctttgtta cgaaagtgtg aaacgattct gttattcctt     1020

cggctatgcc gtaattcaac tttcacaaag cgatcgcgtt ccatcccaat ggcgcgccga     1080

gcttggcgta atcatggtca tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc     1140

cacacaacat acgagccgga agcataaagt gtaaagcctg gggtgcctaa tgagtgagct     1200

aactcacatt aattgcgttg cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc     1260

agctgcatta atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt     1320

ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag     1380

ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca     1440

tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt     1500

tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc     1560

gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct     1620

ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg     1680

tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca     1740

agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact     1800

atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta     1860

acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta     1920

actacggcta cactagaaga acagtatttg gtatctgcgc tctgctgaag ccagttacct     1980

tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt     2040

tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga     2100

tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca     2160

tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat     2220

caatctaaag tatatatgag taaacttggt ctgacagtta ccaatgctta atcagtgagg     2280

cacctatctc agcgatctgt ctatttcgtt catccatagt tgcctgactc cccgtcgtgt     2340

agataactac gatacgggag ggcttaccat ctggccccag tgctgcaatg ataccgcgag     2400

acccacgctc accggctcca gatttatcag caataaacca gccagccgga agggccgagc     2460

gcagaagtgg tcctgcaact ttatccgcct ccatccagtc tattaattgt tgccgggaag     2520

ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt gctacaggca     2580

tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag ctccggttcc caacgatcaa     2640

ggcgagttac atgatccccc atgttgtgca aaaaagcggt tagctccttc ggtcctccga     2700

tcgttgtcag aagtaagttg gccgcagtgt tatcactcat ggttatggca gcactgcata     2760

attctcttac tgtcatgcca tccgtaagat gcttttctgt gactggtgag tactcaacca     2820

agtcattctg agaatagtgt atgcggcgac cgagttgctc ttgcccggcg tcaatacggg     2880

ataataccgc gccacatagc agaactttaa aagtgctcat cattggaaaa cgttcttcgg     2940

ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg     3000

cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga gcaaaaacag     3060

gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga atactcatac     3120

tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg agcggataca     3180

tatttgaatg tatttagaaa aataaacaaa taggggttcc gcgcacattt ccccgaaaag     3240

tgccacctga cgtctaagaa accattatta tcatgacatt aacctataaa aataggcgta     3300

tcacgaggcc ctttcgtc                                                   3318


<210>  3
<211>  3645
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  TIPS repair vector pKVA761

<400>  3
tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca       60

cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg      120

ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc      180

accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc      240

attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat      300

tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt      360

tttcccagtc acgacgttgt aaaacgacgg ccagtgcgat cgcttctccc aaaattatgc      420

aattttgagg ctcctctaca tcattataat tccccaatac attgctcttt attcttaaca      480

gctttgatcg cgaaatttaa cattttaatt cttgagctgt tattttgtag catcagttta      540

tcatgagcca tgtttggtac taaatataca atcccttggg tttatttgtt tccaagcatg      600

tcattaactt atcttaatgt ggacaagaaa ctgatgcctg cttacattgc tattatttca      660

agcgggtatt gatcctttga catgtgattg atcatttttt tttctctggt tattagggca      720

caacagtggt ggacaacttg ctgaacagtg aggatgttca ctacatgctt gaggccctga      780

aagccctcgg gctctctgtg gaagcagata aagttgcaaa aagagctgta gtcgttggct      840

gtggtggcaa gtttcctgtt gagaaggatg cgaaagagga agtgcaactc ttcttgggga      900

acgctggaat tgcaatgcga tcgttgacag cagccgtgac tgctgctggt ggaaatgcaa      960

cgtatgtttt tttttaatgt ttatgaaaat aagtatggaa ttcatggggt atgttttatg     1020

acctttttct ttaccatcag ttatgtgctt gatggagtgc cacgaatgag ggagagaccg     1080

attggtgact tggttgtcgg gttgaaacaa cttggtgcgg atgtcgactg tttccttggc     1140

actgaatgcc cacctgttcg tgtcaaggga attggaggac ttcctggtgg caaggttagt     1200

tactcctaaa ctgcatcctt tgtacttctg tatgcacctc aattctttgt caaccttctg     1260

catttataag gaacattcta tgatgaaatt cgaccttaca ctgcacagta acttgaaatg     1320

tttcatgctt aatcaatatg ccatattcct gccaagctca agcgagcaat atttgtttga     1380

atttggtacc atatttttgt atacctgcag gggcgtaatc atggtcatag ctgtttcctg     1440

tgtgaaattg ttatccgctc acaattccac acaacatacg agccggaagc ataaagtgta     1500

aagcctgggg tgcctaatga gtgagctaac tcacattaat tgcgttgcgc tcactgcccg     1560

ctttccagtc gggaaacctg tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga     1620

gaggcggttt gcgtattggg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg     1680

tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag     1740

aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc     1800

gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca     1860

aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt     1920

ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc     1980

tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc     2040

tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc     2100

ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact     2160

tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg     2220

ctacagagtt cttgaagtgg tggcctaact acggctacac tagaagaaca gtatttggta     2280

tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca     2340

aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa     2400

aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg     2460

aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc     2520

ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg     2580

acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat     2640

ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg     2700

gccccagtgc tgcaatgata ccgcgagacc cacgctcacc ggctccagat ttatcagcaa     2760

taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca     2820

tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc     2880

gcaacgttgt tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt     2940

cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa     3000

aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat     3060

cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct     3120

tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga     3180

gttgctcttg cccggcgtca atacgggata ataccgcgcc acatagcaga actttaaaag     3240

tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga     3300

gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca     3360

ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg     3420

cgacacggaa atgttgaata ctcatactct tcctttttca atattattga agcatttatc     3480

agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag     3540

gggttccgcg cacatttccc cgaaaagtgc cacctgacgt ctaagaaacc attattatca     3600

tgacattaac ctataaaaat aggcgtatca cgaggccctt tcgtc                     3645


<210>  4
<211>  16730
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  T-DNA vector pBAY00461

<400>  4
aattacaacg gtatatatcc tgccagtact cggccgtcga ccgcggtacc ccggaatttt       60

gtggcgctct atcatagcta taaacctatt cagcacaata ttagttagct aggggccccc      120

tcgagggcga tcgcgcggcc gcctgcagtg cagcgtgacc cggtcgtgcc cctctctaga      180

gataatgagc attgcatgtc taagttataa aaaattacca catatttttt ttgtcacact      240

tgtttgaagt gcagtttatc tatctttata catatattta aactttactc tacgaataat      300

ataatctata gtactacaat aatatcagtg ttttagagaa tcatataaat gaacagttag      360

acatggtcta aaggacaatt gagtattttg acaacaggac tctacagttt tatcttttta      420

gtgtgcatgt gttctccttt ttttttgcaa atagcttcac ctatataata cttcatccat      480

tttattagta catccattta gggtttaggg ttaatggttt ttatagacta atttttttag      540

tacatctatt ttattctatt ttagcctcta aattaagaaa actaaaactc tattttagtt      600

tttttattta ataatttaga tataaaatag aataaaataa agtgactaaa aattaaacaa      660

atacccttta agaaattaaa aaaactaagg aaacattttt cttgtttcga gtagataatg      720

ccagcctgtt aaacgccgtc gatcgacgag tctaacggac accaaccagc gaaccagcag      780

cgtcgcgtcg ggccaagcga agcagacggc acggcatctc tgtcgctgcc tctggacccc      840

tctcgagagt tccgctccac cgttggactt gctccgctgt cggcatccag aaattgcgtg      900

gcggagcggc agacgtgagc cggcacggca ggcggcctcc tcctcctctc acggcaccgg      960

cagctacggg ggattccttt cccaccgctc cttcgctttc ccttcctcgc ccgccgtaat     1020

aaatagacac cccctccaca ccctctttcc ccaacctcgt gttgttcgga gcgcacacac     1080

acacaaccag atctccccca aatccacccg tcggcacctc cgcttcaagg tacgccgctc     1140

gtcctccccc cccccccctc tctaccttct ctagatcggc gttccggtcc atgcttaggg     1200

cccggtagtt ctacttctgt ccatgtttgt gttagatccg tgtttgtgtt agatccgtgc     1260

tactagcgtt cgtacacgga tgcgacctgt acgtcagaca cgttctgatt gctaacttgc     1320

cagtgtttct ctttggggaa tcctgggatg gctctagccg ttccgcagac gggatcgatt     1380

tcatgatttt ttttgtttcg ttgcataggg tttggtttgc ccttttcctt tatttcaata     1440

tatgccgtgc acttgtttgt cgggtcatct tttcatgctt ttttttgtct tggttgtgat     1500

gatgtggtct ggttgggcgg tcgttctaga tcggagtaga attctgtttc aaactacctg     1560

gtggatttat taattttgga tctgtatgtg tgtgccatac atattcatag ttacgaattg     1620

aagatgatgg atggaaatat cgatctagga taggtataca tgttgatgcg ggttttactg     1680

atgcatatac agagatgctt tttgttcgct tggttgtgat gatgtggtgt ggttgggcgg     1740

tcgttcattc gttctagatc ggagtagaat actgtttcaa actacctggt gtatttatta     1800

attttggaac tgtatgtgtg tgtcatacat cttcatagtt acgagtttaa gatggatgga     1860

aatatcgatc taggataggt atacatgttg atgtgggttt tactgatgca tatacatgat     1920

ggcatatgca gcatctattc atatgctcta accttgagta cctatctatt ataataaaca     1980

agtatgtttt ataattattt tgatcttgat atacttggat gatggcatat gcagcagcta     2040

tatgtggatt tttttagccc tgccttcata cgctatttat ttgcttggta ctgtttcttt     2100

tgtcgatgct caccctgttg tttggtgtta cttctgcagc catggcccct aagaagaaac     2160

gaaaggtagg catccacggt gttccagcgg ctatggacaa gaagtacagc atcgggctgg     2220

acattggcac gaactctgtt gggtgggcgg ttatcactga cgagtacaag gtgccgtcga     2280

agaagttcaa ggtgctaggc aacaccgacc gtcacagcat caagaagaac ctcatcggcg     2340

ccctgttgtt tgactctggg gaaaccgccg aggctactcg actaaagaga accgcccgta     2400

ggcgatacac taggcggaag aacaggatct gttatctcca ggagatcttc agcaacgaga     2460

tggcgaaggt cgatgactca ttcttccacc gccttgagga gtctttcctg gtcgaggagg     2520

acaaaaagca cgagcgacac ccgatcttcg ggaacatcgt tgatgaggta gcctaccacg     2580

agaagtaccc tactatctac cacctgcgca agaaactagt ggactccacc gacaaggcgg     2640

acctccgcct tatctatctc gccctcgccc acatgatcaa gtttcgcggg cacttcctca     2700

tcgaaggcga tctcaatccg gacaacagcg acgtggacaa gctattcatc cagctagttc     2760

agacctacaa ccagctcttt gaagagaacc ctattaacgc gagcggggtg gacgccaaag     2820

ccatcctttc cgccaggctc tctaagagcc ggagactcga gaaccttatc gctcagttgc     2880

ccggcgagaa gaaaaacggc cttttcggca acctgattgc cctgtccttg ggcttgacgc     2940

cgaacttcaa gagcaacttc gacctagctg aggatgctaa actgcagctg tccaaggaca     3000

cttatgatga cgaccttgac aacctcctgg cccaaattgg ggatcagtac gcggacctct     3060

tcctcgctgc caagaacctc tccgacgcga ttctgttgtc ggacatcctc cgcgtgaaca     3120

ccgagattac taaggcccca ctgtccgcga gcatgatcaa acgctacgac gagcaccatc     3180

aggatctaac tctgctcaag gcgctcgtcc gtcagcaact ccctgagaag tacaaggaga     3240

tcttcttcga ccagtccaag aacggctacg ccggttacat tgatggtggc gcgagccagg     3300

aggagttcta caagttcatc aagcccatct tagagaagat ggacggcacc gaagagcttc     3360

tcgtgaagct caacagggag gacctcctaa ggaagcagcg cacctttgac aacgggtcta     3420

ttcctcatca gatccatctc ggcgagctcc acgcgatttt gagacgccaa gaggacttct     3480

acccgttctt gaaggacaac cgggagaaga tcgagaagat cctcacgttc aggatccctt     3540

attacgtcgg ccccctagct aggggtaact cgagattcgc gtggatgacc agaaagtccg     3600

aggaaaccat taccccgtgg aactttgaag aggtggtcga caagggcgcg agcgcgcaat     3660

cgttcattga gcggatgacc aacttcgaca agaacctacc gaacgagaag gtcctgccga     3720

aacactccct gctctacgag tacttcaccg tttataacga gctgacgaag gtcaagtacg     3780

tgactgaggg catgcgtaaa ccagcgttcc tcagcggtga gcagaagaag gctatcgtgg     3840

acctgctctt taagacgaac cgcaaggtga ccgtgaagca gctcaaggag gattatttca     3900

agaagatcga gtgcttcgac tcagtggaga ttagcggcgt ggaggaccgt ttcaatgcga     3960

gcttaggtac ctaccacgac ctcctgaaga tcatcaagga caaggacttc ctcgacaacg     4020

aggaaaacga ggacatcctg gaggacatcg tgctcaccct gaccctcttt gaagacaggg     4080

agatgatcga ggaaaggttg aagacctacg cgcacctatt cgacgacaag gtcatgaaac     4140

agctcaagcg gaggagatac actggctggg gcagactttc gcggaagctc atcaatggga     4200

tcagggacaa gcagtccggc aagaccatcc tggacttctt gaagagcgac ggcttcgcta     4260

atcgcaactt catgcagctc attcatgacg actccctaac attcaaggag gacatccaaa     4320

aggcgcaagt ctcgggtcag ggggattctt tgcacgagca catcgcaaac ctcgccggtt     4380

ctccggccat taaaaagggc atccttcaga cggtcaaggt ggtcgacgag ctcgtgaagg     4440

ttatgggccg gcataagccc gagaacatag tgatcgagat ggctagggag aaccagacca     4500

cgcaaaaggg ccagaagaac tcacgtgagc gcatgaagag gatcgaggaa ggtatcaagg     4560

agctgggctc tcagattctc aaggagcatc cggtcgagaa cacccagctt cagaacgaga     4620

agctgtacct ctactacctc cagaatggcc gcgacatgta cgttgatcag gagttagata     4680

ttaatcggct gagcgactac gacgttgacc acatcgtgcc ccagtcgttc ctcaaggatg     4740

actccatcga caacaaggtc ctgaccagat cggacaagaa ccgtggtaag agcgacaatg     4800

tgccctccga ggaggtggtc aaaaagatga agaactattg gcggcagttg ctgaacgcca     4860

agctcatcac ccagcgaaag ttcgataatt taacgaaggc cgaacgaggg ggcctttccg     4920

aactcgataa ggccggtttc atcaagcgtc agctggttga gacgcgacag atcacgaagc     4980

atgtggccca gattctggac agccggatga acacgaagta cgacgagaac gacaaactta     5040

ttcgggaggt gaaggtgata accctcaagt ccaagttagt gtcggacttc cgcaaggact     5100

ttcagttcta taaagtgcgc gagatcaaca attaccacca cgcccacgac gcctacttga     5160

atgccgtggt cggcacggcg ctcatcaaaa agtaccccaa gctggagagc gagttcgtct     5220

acggcgacta caaggtttat gacgtcagga agatgattgc gaagtcagag caagagatcg     5280

ggaaggccac cgccaagtac ttcttctact cgaacatcat gaacttcttt aagaccgaga     5340

tcaccctcgc caatggggag atccggaaac gtccccttat cgagacgaac ggcgagactg     5400

gcgagatagt ctgggacaaa ggtcgcgatt tcgccaccgt tcgaaaggtg ctctcaatgc     5460

ctcaggtcaa tatcgtgaag aagaccgagg ttcagacggg gggcttcagc aaggagtcca     5520

tcctccctaa gcgcaactct gacaagctga tcgcccggaa aaaggattgg gacccgaaaa     5580

agtacggcgg cttcgattct cccaccgtag cttatagcgt gctcgtggtt gccaaggtgg     5640

agaagggcaa gagcaagaaa ctgaagtcgg tcaaggagtt actcggcatc acaatcatgg     5700

agcgcagctc gtttgaaaag aacccgatcg acttcctgga ggccaagggc tataaggagg     5760

tgaagaagga cctcatcatt aagctgccga agtacagctt gttcgagctc gagaatggca     5820

ggaagaggat gctagcttcg gccggcgaac ttcaaaaagg gaacgagctc gcgttgccgt     5880

cgaagtacgt gaacttcctc tacttagcta gtcactatga aaagctgaag ggctcgccgg     5940

aggacaacga gcagaagcag ctcttcgtcg agcaacacaa gcactacctc gacgagatca     6000

tcgagcagat aagtgagttc agcaagcgcg tgatcctcgc tgatgccaac ctcgataagg     6060

tgctcagcgc ctacaataag caccgagaca agccgattcg ggagcaggcg gagaacatca     6120

ttcatttatt tacgctcact aacctaggcg ctccggctgc cttcaagtac ttcgacacca     6180

cgatcgacag gaagcgctac accagcacca aagaagtcct cgacgcgacg ctcattcacc     6240

agagcatcac agggctctac gagactcgga tcgacttgtc gcaattgggt ggggataaga     6300

ggccggccgc gactaaaaag gccggccaag ccaagaaaaa gaaatgattg ctagcacgcg     6360

tcgaagcaga tcgttcaaac atttggcaat aaagtttctt aagattgaat cctgttgccg     6420

gtcttgcgat gattatcata taatttctgt tgaattacgt taagcatgta ataattaaca     6480

tgtaatgcat gacgttattt atgagatggg tttttatgat tagagtcccg caattataca     6540

tttaatacgc gatagaaaac aaaatatagc gcgcaaacta ggataaatta tcgcgcgcgg     6600

tgtcatctat gttactagat ccctgcaggt ttgtgaaagt tgaattacgg catagccgaa     6660

ggaataacag aatcgtttca cactttcgta acaaaggtct tcttatcatg tttcagacga     6720

tggaggcaag gctgatcaaa gtgatcaagc acataaacgc atttttttac catgtttcac     6780

tccataagcg tctgagatta tcacaagtca cgtctagtag tttgatggta cactagtgac     6840

aatcagttcg tgcagacaga gctcatactt gactacttga gcgattacag gcgaaagtgt     6900

gaaacgcatg tgatgtgggc tgggaggagg agaatatata ctaatgggcc gtatcctgat     6960

ttgggctgcg tcggaaggtg cagcccacgc gcgccgtacc gcgcgggtgg cgctgctacc     7020

cactttagtc cgttggatgg ggatccgatg gtttgcgcgg tggcgttgcg ggggatgttt     7080

agtaccacat cggaaaccga aagacgatgg aaccagctta taaacccgcg cgctgtagtc     7140

agcttgcagt cacggctgct gtcaagtttt agagctagaa atagcaagtt aaaataaggc     7200

tagtccgtta tcaacttgaa aaagtggcac cgagtcggtg cttttttttt aattaattct     7260

cccaaaatta tgcaattttg aggctcctct acatcattat aattccccaa tacattgctc     7320

tttattctta acagctttga tcgcgaaatt taacatttta attcttgagc tgttattttg     7380

tagcatcagt ttatcatgag ccatgtttgg tactaaatat acaatccctt gggtttattt     7440

gtttccaagc atgtcattaa cttatcttaa tgtggacaag aaactgatgc ctgcttacat     7500

tgctattatt tcaagcgggt attgatcctt tgacatgtga ttgatcattt ttttttctct     7560

ggttattagg gcacaacagt ggtggacaac ttgctgaaca gtgaggatgt tcactacatg     7620

cttgaggccc tgaaagccct cgggctctct gtggaagcag ataaagttgc aaaaagagct     7680

gtagtcgttg gctgtggtgg caagtttcct gttgagaagg atgcgaaaga ggaagtgcaa     7740

ctcttcttgg ggaacgctgg aattgcaatg cgatcgttga cagcagccgt gactgctgct     7800

ggtggaaatg caacgtatgt ttttttttaa tgtttatgaa aataagtatg gaattcatgg     7860

ggtatgtttt atgacctttt tctttaccat cagttatgtg cttgatggag tgccacgaat     7920

gagggagaga ccgattggtg acttggttgt cgggttgaaa caacttggtg cggatgtcga     7980

ctgtttcctt ggcactgaat gcccacctgt tcgtgtcaag ggaattggag gacttcctgg     8040

tggcaaggtt agttactcct aaactgcatc ctttgtactt ctgtatgcac ctcaattctt     8100

tgtcaacctt ctgcatttat aaggaacatt ctatgatgaa attcgacctt acactgcaca     8160

gtaacttgaa atgtttcatg cttaatcaat atgccatatt cctgccaagc tcaagcgagc     8220

aatatttgtt tgaatttggt accatatttt tgtataggcg cgccgaagca gatcgttcaa     8280

acatttggca ataaagtttc ttaagattga atcctgttgc cggtcttgcg atgattatca     8340

tataatttct gttgaattac gttaagcatg taataattaa catgtaatgc atgacgttat     8400

ttatgagatg ggtttttatg attagagtcc cgcaattata catttaatac gcgatagaaa     8460

acaaaatata gcgcgcaaac taggataaat tatcgcgcgc ggtgtcatct atgttactag     8520

atcggaattc gatatcatta ccctgttatc cctaaagctt attaatataa cttcgtatag     8580

catacattat acgaagttat gtttcctacg cagcaggtct catcaagacg atctacccga     8640

gtaacaatct ccaggagatc aaataccttc ccaagaaggt taaagatgca gtcaaaagat     8700

tcaggactaa ttgcatcaag aacacagaga aagacatatt tctcaagatc agaagtacta     8760

ttccagtatg gacgattcaa ggcttgcttc ataaaccaag gcaagtaata gagattggag     8820

tctctaaaaa ggtagttcct actgaatcta aggccatgca tggagtctaa gattcaaatc     8880

gaggatctaa cagaactcgc cgtgaagact ggcgaacagt tcatacagag tcttttacga     8940

ctcaatgaca agaagaaaat cttcgtcaac atggtggagc acgacactct ggtctactcc     9000

aaaaatgtca aagatacagt ctcagaagac caaagggcta ttgagacttt tcaacaaagg     9060

ataatttcgg gaaacctcct cggattccat tgcccagcta tctgtcactt catcgaaagg     9120

acagtagaaa aggaaggtgg ctcctacaaa tgccatcatt gcgataaagg aaaggctatc     9180

attcaagatg cctctgccga cagtggtccc aaagatggac ccccacccac gaggagcatc     9240

gtggaaaaag aagacgttcc aaccacgtct tcaaagcaag tggattgatg tgacatctcc     9300

actgacgtaa gggatgacgc acaatcccac tatccttcgc aagacccttc ctctatataa     9360

ggaagttcat ttcatttgga gaggacacgc tgaaatcacc agtctctctc tataaatcta     9420

tctctctctc tataacaatg gacccagaac gacgcccggc cgacatccgc cgtgccaccg     9480

aggcggacat gccggcggtc tgcaccatcg tcaaccacta catcgagaca agcacggtca     9540

acttccgtac cgagccgcag gaaccgcagg agtggacgga cgacctcgtc cgtctgcggg     9600

agcgctatcc ctggctcgtc gccgaggtgg acggcgaggt cgccggcatc gcctacgcgg     9660

gcccctggaa ggcacgcaac gcctacgact ggacggccga gtcgaccgtg tacgtctccc     9720

cccgccacca gcggacggga ctgggctcca cgctctacac ccacctgctg aagtccctgg     9780

aggcacaggg cttcaagagc gtggtcgctg tcatcgggct gcccaacgac ccgagcgtgc     9840

gcatgcacga ggcgctcgga tatgcccccc gcggcatgct gcgggcggcc ggcttcaagc     9900

acgggaactg gcatgacgtg ggtttctggc agctggactt cagcctgccg gtaccgcccc     9960

gtccggtcct gcccgtcacc gagatctgag atcacccgtt ctaggatccg aagcagatcg    10020

ttcaaacatt tggcaataaa gtttcttaag attgaatcct gttgccggtc ttgcgatgat    10080

tatcatataa tttctgttga attacgttaa gcatgtaata attaacatgt aatgcatgac    10140

gttatttatg agatgggttt ttatgattag agtcccgcaa ttatacattt aatacgcgat    10200

agaaaacaaa atatagcgcg caaactagga taaattatcg cgcgcggtgt catctatgtt    10260

actagatcga aacataactt cgtatagcat acattatacg aagttatcaa aacgtcgtga    10320

gacagtttgg ttaactataa cggtcctaag gtagcgatta gttagctagc gagcggcgaa    10380

ctaataactc cgctctaccg aaagttactc gaggcattac ggcattacgg cactcgcgag    10440

ggtcccaatt cgagcatgga gccatttaca attgaatata tcctgccgcc gctgccgctt    10500

tgcacccggt ggagcttgca tgttggtttc tacgcagaac tgagccggtt aggcagataa    10560

tttccattga gaactgagcc atgtgcacct tccccccaac acggtgagcg acggggcaac    10620

ggagtgatcc acatgggact tttaaacatc atccgtcgga tggcgttgcg agagaagcag    10680

tcgatccgtg agatcagccg acgcaccggg caggcgcgca acacgatcgc aaagtatttg    10740

aacgcaggta caatcgagcc gacgttcacg gtaccggaac gaccaagcaa gctagcttag    10800

taaagccctc gctagatttt aatgcggatg ttgcgattac ttcgccaact attgcgataa    10860

caagaaaaag ccagcctttc atgatatatc tcccaatttg tgtagggctt attatgcacg    10920

cttaaaaata ataaaagcag acttgacctg atagtttggc tgtgagcaat tatgtgctta    10980

gtgcatctaa cgcttgagtt aagccgcgcc gcgaagcggc gtcggcttga acgaattgtt    11040

agacattatt tgccgactac cttggtgatc tcgcctttca cgtagtggac aaattcttcc    11100

aactgatctg cgcgcgaggc caagcgatct tcttcttgtc caagataagc ctgtctagct    11160

tcaagtatga cgggctgata ctgggccggc aggcgctcca ttgcccagtc ggcagcgaca    11220

tccttcggcg cgattttgcc ggttactgcg ctgtaccaaa tgcgggacaa cgtaagcact    11280

acatttcgct catcgccagc ccagtcgggc ggcgagttcc atagcgttaa ggtttcattt    11340

agcgcctcaa atagatcctg ttcaggaacc ggatcaaaga gttcctccgc cgctggacct    11400

accaaggcaa cgctatgttc tcttgctttt gtcagcaaga tagccagatc aatgtcgatc    11460

gtggctggct cgaagatacc tgcaagaatg tcattgcgct gccattctcc aaattgcagt    11520

tcgcgcttag ctggataacg ccacggaatg atgtcgtcgt gcacaacaat ggtgacttct    11580

acagcgcgga gaatctcgct ctctccaggg gaagccgaag tttccaaaag gtcgttgatc    11640

aaagctcgcc gcgttgtttc atcaagcctt acggtcaccg taaccagcaa atcaatatca    11700

ctgtgtggct tcaggccgcc atccactgcg gagccgtaca aatgtacggc cagcaacgtc    11760

ggttcgagat ggcgctcgat gacgccaact acctctgata gttgagtcga tacttcggcg    11820

atcaccgctt ccctcatgat gtttaacttt gttttagggc gactgccctg ctgcgtaaca    11880

tcgttgctgc tccataacat caaacatcga cccacggcgt aacgcgcttg ctgcttggat    11940

gcccgaggca tagactgtac cccaaaaaaa cagtcataac aagccatgaa aaccgccact    12000

gcgccgttac caccgctgcg ttcggtcaag gttctggaca gttgcgtgag cgcatacgct    12060

acttgcatta cagcttacga accgaacagg cttatgtcca ctgggttcgt gccttcatcc    12120

gtttccacgg tgtgcgtcac ccggcaacct tgggcagcag cgaagtcgag gcatttctgt    12180

cctggctggc gaacgagcgc aaggtttcgg tctccacgca tcgtcaggca ttggcggcct    12240

tgctgttctt ctacggcaag gtgctgtgca cggatctgcc ctggcttcag gagatcggaa    12300

gacctcggcc gtcgcggcgc ttgccggtgg tgctgacccc ggatgaagtc tctagagctc    12360

tagagggttc gcatcctcgg ttttctggaa ggcgagcatc gtttgttcgc ccagcttctg    12420

tatggaacgg gcatgcggat cagtgagggt ttgcaactgc gggtcaagga tctggatttc    12480

gatcacggca cgatcatcgt gcgggagggc aagggctcca aggatcgggc cttgatgtta    12540

cccgagagct tggcacccag cctgcgcgag caggatcgat ccgtgcggct gcatgaaatc    12600

ctggccggtt tgtctgatgc caagctggcg gcctggccgg ccagcttggc cgctgaagaa    12660

accgagcgcc gccgtctaaa aaggtgatgt gtatttgagt aaaacagctt gcgtcatgcg    12720

gtcgctgcgt atatgatgcg atgagtaaat aaacaaatac gcaaggggaa cgcatgaagg    12780

ttatcgctgt acttaaccag aaaggcgggt caggcaagac gaccatcgca acccatctag    12840

cccgcgccct gcaactcgcc ggggccgatg ttctgttagt cgattccgat ccccagggca    12900

gtgcccgcga ttgggcggcc gtgcgggaag atcaaccgct aaccgttgtc ggcatcgacc    12960

gcccgacgat tgaccgcgac gtgaaggcca tcggccggcg cgacttcgta gtgatcgacg    13020

gagcgcccca ggcggcggac ttggctgtgt ccgcgatcaa ggcagccgac ttcgtgctga    13080

ttccggtgca gccaagccct tacgacatat gggccaccgc cgacctggtg gagctggtta    13140

agcagcgcat tgaggtcacg gatggaaggc tacaagcggc ctttgtcgtg tcgcgggcga    13200

tcaaaggcac gcgcatcggc ggtgaggttg ccgaggcgct ggccgggtac gagctgccca    13260

ttcttgagtc ccgtatcacg cagcgcgtga gctacccagg cactgccgcc gccggcacaa    13320

ccgttcttga atcagaaccc gagggcgacg ctgcccgcga ggtccaggcg ctggccgctg    13380

aaattaaatc aaaactcatt tgagttaatg aggtaaagag aaaatgagca aaagcacaaa    13440

cacgctaagt gccggccgtc cgagcgcacg cagcagcaag gctgcaacgt tggccagcct    13500

ggcagacacg ccagccatga agcgggtcaa ctttcagttg ccggcggagg atcacaccaa    13560

gctgaagatg tacgcggtac gccaaggcaa gaccattacc gagctgctat ctgaatacat    13620

cgcgcagcta ccagagtaaa tgagcaaatg aataaatgag tagatgaatt ttagcggcta    13680

aaggaggcgg catggaaaat caagaacaac caggcaccga cgccgtggaa tgccccatgt    13740

gtggaggaac gggcggttgg ccaggcgtaa gcggctgggt tgtctgccgg ccctgcaatg    13800

gcactggaac ccccaagccc gaggaatcgg cgtgagcggt cgcaaaccat ccggcccggt    13860

acaaatcggc gcggcgctgg gtgatgacct ggtggagaag ttgaaggccg cgcaggccgc    13920

ccagcggcaa cgcatcgagg cagaagcacg ccccggtgaa tcgtggcaag cggccgctga    13980

tcgaatccgc aaagaatccc ggcaaccgcc ggcagccggt gcgccgtcga ttaggaagcc    14040

gcccaagggc gacgagcaac cagatttttt cgttccgatg ctctatgacg tgggcacccg    14100

cgatagtcgc agcatcatgg acgtggccgt tttccgtctg tcgaagcgtg accgacgagc    14160

tggcgaggtg atccgctacg agcttccaga cgggcacgta gaggtttccg cagggccggc    14220

cggcatggcc agtgtgtggg attacgacct ggtactgatg gcggtttccc atctaaccga    14280

atccatgaac cgataccggg aagggaaggg agacaagccc ggccgcgtgt tccgtccaca    14340

cgttgcggac gtactcaagt tctgccggcg agccgatggc ggaaagcaga aagacgacct    14400

ggtagaaacc tgcattcggt taaacaccac gcacgttgcc atgcagcgta cgaagaaggc    14460

caagaacggc cgcctggtga cggtatccga gggtgaagcc ttgattagcc gctacaagat    14520

cgtaaagagc gaaaccgggc ggccggagta catcgagatc gagctagctg attggatgta    14580

ccgcgagatc acagaaggca agaacccgga cgtgctgacg gttcaccccg attacttttt    14640

gatcgatccc ggcatcggcc gttttctcta ccgcctggca cgccgcgccg caggcaaggc    14700

agaagccaga tggttgttca agacgatcta cgaacgcagt ggcagcgccg gagagttcaa    14760

gaagttctgt ttcaccgtgc gcaagctgat cgggtcaaat gacctgccgg agtacgattt    14820

gaaggaggag gcggggcagg ctggcccgat cctagtcatg cgctaccgca acctgatcga    14880

gggcgaagca tccgccggtt cctaatgtac ggagcagatg ctagggcaaa ttgccctagc    14940

aggggaaaaa ggtcgaaaag gtctctttcc tgtggatagc acgtacattg ggaacccaaa    15000

gccgtacatt gggaaccgga acccgtacat tgggaaccca aagccgtaca ttgggaaccg    15060

gtcacacatg taagtgactg atataaaaga gaaaaaaggc gatttttccg cctaaaactc    15120

tttaaaactt attaaaactc ttaaaacccg cctggcctgt gcataactgt ctggccagcg    15180

cacagccgaa gagctgcaaa aagcgcctac ccttcggtcg ctgcgctccc tacgccccgc    15240

cgcttcgcgt cggcctatcg cggccgctgg ccgctcaaaa atggctggcc tacggccagg    15300

caatctacca gggcgcggac aagccgcgcc gtcgccactc gaccgccggc gcccacatca    15360

aggcaccctg cctcgcgcgt ttcggtgatg acggtgaaaa cctctgacac atgcagctcc    15420

cggagacggt cacagcttgt ctgtaagcgg atgccgggag cagacaagcc cgtcagggcg    15480

cgtcagcggg tgttggcggg tgtcggggcg cagccatgac ccagtcacgt agcgatagcg    15540

gagtgtatac tggcttaact atgcggcatc agagcagatt gtactgagag tgcaccatat    15600

gcggtgtgaa ataccgcaca gatgcgtaag gagaaaatac cgcatcaggc gctcttccgc    15660

ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca    15720

ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg    15780

agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca    15840

taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa    15900

cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc    15960

tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc    16020

gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct    16080

gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg    16140

tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag    16200

gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta    16260

cggctacact agaaggacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg    16320

aaaaagagtt gatagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt    16380

tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc cggaaaacgc    16440

aagcgcaaag agaaagcagg tagcttgcag tgggcttaca tggcgatagc tagactgggc    16500

ggttttatgg acagcaagcg aaccggaatt gccagattcg aagctcggtc ccgtgggtgt    16560

tctgtcgtct cgttgtacaa cgaaatccat tcccattccg cgctcaagat ggcttcccct    16620

cggcagttca tcagggctaa atcaatctag ccgacttgtc cggtgaaatg ggctgcactc    16680

caacagaaac aatcaaacaa acatacacag cgacttattc acacgcgaca               16730


<210>  5
<211>  4206
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Modified Cas 9 coding region comprising nuclear localization 
       signals and codon optimized


<220>
<221>  misc_feature
<222>  (10)..(27)
<223>  nuclear localization signal Large T-antigen SV40

<220>
<221>  misc_feature
<222>  (4165)..(4203)
<223>  nuclear localization signal nucleoplasmin

<400>  5
atggccccta agaagaaacg aaaggtaggc atccacggtg ttccagcggc tatggacaag       60

aagtacagca tcgggctgga cattggcacg aactctgttg ggtgggcggt tatcactgac      120

gagtacaagg tgccgtcgaa gaagttcaag gtgctaggca acaccgaccg tcacagcatc      180

aagaagaacc tcatcggcgc cctgttgttt gactctgggg aaaccgccga ggctactcga      240

ctaaagagaa ccgcccgtag gcgatacact aggcggaaga acaggatctg ttatctccag      300

gagatcttca gcaacgagat ggcgaaggtc gatgactcat tcttccaccg ccttgaggag      360

tctttcctgg tcgaggagga caaaaagcac gagcgacacc cgatcttcgg gaacatcgtt      420

gatgaggtag cctaccacga gaagtaccct actatctacc acctgcgcaa gaaactagtg      480

gactccaccg acaaggcgga cctccgcctt atctatctcg ccctcgccca catgatcaag      540

tttcgcgggc acttcctcat cgaaggcgat ctcaatccgg acaacagcga cgtggacaag      600

ctattcatcc agctagttca gacctacaac cagctctttg aagagaaccc tattaacgcg      660

agcggggtgg acgccaaagc catcctttcc gccaggctct ctaagagccg gagactcgag      720

aaccttatcg ctcagttgcc cggcgagaag aaaaacggcc ttttcggcaa cctgattgcc      780

ctgtccttgg gcttgacgcc gaacttcaag agcaacttcg acctagctga ggatgctaaa      840

ctgcagctgt ccaaggacac ttatgatgac gaccttgaca acctcctggc ccaaattggg      900

gatcagtacg cggacctctt cctcgctgcc aagaacctct ccgacgcgat tctgttgtcg      960

gacatcctcc gcgtgaacac cgagattact aaggccccac tgtccgcgag catgatcaaa     1020

cgctacgacg agcaccatca ggatctaact ctgctcaagg cgctcgtccg tcagcaactc     1080

cctgagaagt acaaggagat cttcttcgac cagtccaaga acggctacgc cggttacatt     1140

gatggtggcg cgagccagga ggagttctac aagttcatca agcccatctt agagaagatg     1200

gacggcaccg aagagcttct cgtgaagctc aacagggagg acctcctaag gaagcagcgc     1260

acctttgaca acgggtctat tcctcatcag atccatctcg gcgagctcca cgcgattttg     1320

agacgccaag aggacttcta cccgttcttg aaggacaacc gggagaagat cgagaagatc     1380

ctcacgttca ggatccctta ttacgtcggc cccctagcta ggggtaactc gagattcgcg     1440

tggatgacca gaaagtccga ggaaaccatt accccgtgga actttgaaga ggtggtcgac     1500

aagggcgcga gcgcgcaatc gttcattgag cggatgacca acttcgacaa gaacctaccg     1560

aacgagaagg tcctgccgaa acactccctg ctctacgagt acttcaccgt ttataacgag     1620

ctgacgaagg tcaagtacgt gactgagggc atgcgtaaac cagcgttcct cagcggtgag     1680

cagaagaagg ctatcgtgga cctgctcttt aagacgaacc gcaaggtgac cgtgaagcag     1740

ctcaaggagg attatttcaa gaagatcgag tgcttcgact cagtggagat tagcggcgtg     1800

gaggaccgtt tcaatgcgag cttaggtacc taccacgacc tcctgaagat catcaaggac     1860

aaggacttcc tcgacaacga ggaaaacgag gacatcctgg aggacatcgt gctcaccctg     1920

accctctttg aagacaggga gatgatcgag gaaaggttga agacctacgc gcacctattc     1980

gacgacaagg tcatgaaaca gctcaagcgg aggagataca ctggctgggg cagactttcg     2040

cggaagctca tcaatgggat cagggacaag cagtccggca agaccatcct ggacttcttg     2100

aagagcgacg gcttcgctaa tcgcaacttc atgcagctca ttcatgacga ctccctaaca     2160

ttcaaggagg acatccaaaa ggcgcaagtc tcgggtcagg gggattcttt gcacgagcac     2220

atcgcaaacc tcgccggttc tccggccatt aaaaagggca tccttcagac ggtcaaggtg     2280

gtcgacgagc tcgtgaaggt tatgggccgg cataagcccg agaacatagt gatcgagatg     2340

gctagggaga accagaccac gcaaaagggc cagaagaact cacgtgagcg catgaagagg     2400

atcgaggaag gtatcaagga gctgggctct cagattctca aggagcatcc ggtcgagaac     2460

acccagcttc agaacgagaa gctgtacctc tactacctcc agaatggccg cgacatgtac     2520

gttgatcagg agttagatat taatcggctg agcgactacg acgttgacca catcgtgccc     2580

cagtcgttcc tcaaggatga ctccatcgac aacaaggtcc tgaccagatc ggacaagaac     2640

cgtggtaaga gcgacaatgt gccctccgag gaggtggtca aaaagatgaa gaactattgg     2700

cggcagttgc tgaacgccaa gctcatcacc cagcgaaagt tcgataattt aacgaaggcc     2760

gaacgagggg gcctttccga actcgataag gccggtttca tcaagcgtca gctggttgag     2820

acgcgacaga tcacgaagca tgtggcccag attctggaca gccggatgaa cacgaagtac     2880

gacgagaacg acaaacttat tcgggaggtg aaggtgataa ccctcaagtc caagttagtg     2940

tcggacttcc gcaaggactt tcagttctat aaagtgcgcg agatcaacaa ttaccaccac     3000

gcccacgacg cctacttgaa tgccgtggtc ggcacggcgc tcatcaaaaa gtaccccaag     3060

ctggagagcg agttcgtcta cggcgactac aaggtttatg acgtcaggaa gatgattgcg     3120

aagtcagagc aagagatcgg gaaggccacc gccaagtact tcttctactc gaacatcatg     3180

aacttcttta agaccgagat caccctcgcc aatggggaga tccggaaacg tccccttatc     3240

gagacgaacg gcgagactgg cgagatagtc tgggacaaag gtcgcgattt cgccaccgtt     3300

cgaaaggtgc tctcaatgcc tcaggtcaat atcgtgaaga agaccgaggt tcagacgggg     3360

ggcttcagca aggagtccat cctccctaag cgcaactctg acaagctgat cgcccggaaa     3420

aaggattggg acccgaaaaa gtacggcggc ttcgattctc ccaccgtagc ttatagcgtg     3480

ctcgtggttg ccaaggtgga gaagggcaag agcaagaaac tgaagtcggt caaggagtta     3540

ctcggcatca caatcatgga gcgcagctcg tttgaaaaga acccgatcga cttcctggag     3600

gccaagggct ataaggaggt gaagaaggac ctcatcatta agctgccgaa gtacagcttg     3660

ttcgagctcg agaatggcag gaagaggatg ctagcttcgg ccggcgaact tcaaaaaggg     3720

aacgagctcg cgttgccgtc gaagtacgtg aacttcctct acttagctag tcactatgaa     3780

aagctgaagg gctcgccgga ggacaacgag cagaagcagc tcttcgtcga gcaacacaag     3840

cactacctcg acgagatcat cgagcagata agtgagttca gcaagcgcgt gatcctcgct     3900

gatgccaacc tcgataaggt gctcagcgcc tacaataagc accgagacaa gccgattcgg     3960

gagcaggcgg agaacatcat tcatttattt acgctcacta acctaggcgc tccggctgcc     4020

ttcaagtact tcgacaccac gatcgacagg aagcgctaca ccagcaccaa agaagtcctc     4080

gacgcgacgc tcattcacca gagcatcaca gggctctacg agactcggat cgacttgtcg     4140

caattgggtg gggataagag gccggccgcg actaaaaagg ccggccaagc caagaaaaag     4200

aaatga                                                                4206


<210>  6
<211>  1401
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Modified Cas 9 protein comprising nuclear localization signals


<220>
<221>  MISC_FEATURE
<222>  (4)..(9)
<223>  nuclear localization signal large T-antigen SV40

<220>
<221>  MISC_FEATURE
<222>  (1389)..(1401)
<223>  nuclear localization signal large nucleoplasmin

<400>  6

Met Ala Pro Lys Lys Lys Arg Lys Val Gly Ile His Gly Val Pro Ala 
1               5                   10                  15      


Ala Met Asp Lys Lys Tyr Ser Ile Gly Leu Asp Ile Gly Thr Asn Ser 
            20                  25                  30          


Val Gly Trp Ala Val Ile Thr Asp Glu Tyr Lys Val Pro Ser Lys Lys 
        35                  40                  45              


Phe Lys Val Leu Gly Asn Thr Asp Arg His Ser Ile Lys Lys Asn Leu 
    50                  55                  60                  


Ile Gly Ala Leu Leu Phe Asp Ser Gly Glu Thr Ala Glu Ala Thr Arg 
65                  70                  75                  80  


Leu Lys Arg Thr Ala Arg Arg Arg Tyr Thr Arg Arg Lys Asn Arg Ile 
                85                  90                  95      


Cys Tyr Leu Gln Glu Ile Phe Ser Asn Glu Met Ala Lys Val Asp Asp 
            100                 105                 110         


Ser Phe Phe His Arg Leu Glu Glu Ser Phe Leu Val Glu Glu Asp Lys 
        115                 120                 125             


Lys His Glu Arg His Pro Ile Phe Gly Asn Ile Val Asp Glu Val Ala 
    130                 135                 140                 


Tyr His Glu Lys Tyr Pro Thr Ile Tyr His Leu Arg Lys Lys Leu Val 
145                 150                 155                 160 


Asp Ser Thr Asp Lys Ala Asp Leu Arg Leu Ile Tyr Leu Ala Leu Ala 
                165                 170                 175     


His Met Ile Lys Phe Arg Gly His Phe Leu Ile Glu Gly Asp Leu Asn 
            180                 185                 190         


Pro Asp Asn Ser Asp Val Asp Lys Leu Phe Ile Gln Leu Val Gln Thr 
        195                 200                 205             


Tyr Asn Gln Leu Phe Glu Glu Asn Pro Ile Asn Ala Ser Gly Val Asp 
    210                 215                 220                 


Ala Lys Ala Ile Leu Ser Ala Arg Leu Ser Lys Ser Arg Arg Leu Glu 
225                 230                 235                 240 


Asn Leu Ile Ala Gln Leu Pro Gly Glu Lys Lys Asn Gly Leu Phe Gly 
                245                 250                 255     


Asn Leu Ile Ala Leu Ser Leu Gly Leu Thr Pro Asn Phe Lys Ser Asn 
            260                 265                 270         


Phe Asp Leu Ala Glu Asp Ala Lys Leu Gln Leu Ser Lys Asp Thr Tyr 
        275                 280                 285             


Asp Asp Asp Leu Asp Asn Leu Leu Ala Gln Ile Gly Asp Gln Tyr Ala 
    290                 295                 300                 


Asp Leu Phe Leu Ala Ala Lys Asn Leu Ser Asp Ala Ile Leu Leu Ser 
305                 310                 315                 320 


Asp Ile Leu Arg Val Asn Thr Glu Ile Thr Lys Ala Pro Leu Ser Ala 
                325                 330                 335     


Ser Met Ile Lys Arg Tyr Asp Glu His His Gln Asp Leu Thr Leu Leu 
            340                 345                 350         


Lys Ala Leu Val Arg Gln Gln Leu Pro Glu Lys Tyr Lys Glu Ile Phe 
        355                 360                 365             


Phe Asp Gln Ser Lys Asn Gly Tyr Ala Gly Tyr Ile Asp Gly Gly Ala 
    370                 375                 380                 


Ser Gln Glu Glu Phe Tyr Lys Phe Ile Lys Pro Ile Leu Glu Lys Met 
385                 390                 395                 400 


Asp Gly Thr Glu Glu Leu Leu Val Lys Leu Asn Arg Glu Asp Leu Leu 
                405                 410                 415     


Arg Lys Gln Arg Thr Phe Asp Asn Gly Ser Ile Pro His Gln Ile His 
            420                 425                 430         


Leu Gly Glu Leu His Ala Ile Leu Arg Arg Gln Glu Asp Phe Tyr Pro 
        435                 440                 445             


Phe Leu Lys Asp Asn Arg Glu Lys Ile Glu Lys Ile Leu Thr Phe Arg 
    450                 455                 460                 


Ile Pro Tyr Tyr Val Gly Pro Leu Ala Arg Gly Asn Ser Arg Phe Ala 
465                 470                 475                 480 


Trp Met Thr Arg Lys Ser Glu Glu Thr Ile Thr Pro Trp Asn Phe Glu 
                485                 490                 495     


Glu Val Val Asp Lys Gly Ala Ser Ala Gln Ser Phe Ile Glu Arg Met 
            500                 505                 510         


Thr Asn Phe Asp Lys Asn Leu Pro Asn Glu Lys Val Leu Pro Lys His 
        515                 520                 525             


Ser Leu Leu Tyr Glu Tyr Phe Thr Val Tyr Asn Glu Leu Thr Lys Val 
    530                 535                 540                 


Lys Tyr Val Thr Glu Gly Met Arg Lys Pro Ala Phe Leu Ser Gly Glu 
545                 550                 555                 560 


Gln Lys Lys Ala Ile Val Asp Leu Leu Phe Lys Thr Asn Arg Lys Val 
                565                 570                 575     


Thr Val Lys Gln Leu Lys Glu Asp Tyr Phe Lys Lys Ile Glu Cys Phe 
            580                 585                 590         


Asp Ser Val Glu Ile Ser Gly Val Glu Asp Arg Phe Asn Ala Ser Leu 
        595                 600                 605             


Gly Thr Tyr His Asp Leu Leu Lys Ile Ile Lys Asp Lys Asp Phe Leu 
    610                 615                 620                 


Asp Asn Glu Glu Asn Glu Asp Ile Leu Glu Asp Ile Val Leu Thr Leu 
625                 630                 635                 640 


Thr Leu Phe Glu Asp Arg Glu Met Ile Glu Glu Arg Leu Lys Thr Tyr 
                645                 650                 655     


Ala His Leu Phe Asp Asp Lys Val Met Lys Gln Leu Lys Arg Arg Arg 
            660                 665                 670         


Tyr Thr Gly Trp Gly Arg Leu Ser Arg Lys Leu Ile Asn Gly Ile Arg 
        675                 680                 685             


Asp Lys Gln Ser Gly Lys Thr Ile Leu Asp Phe Leu Lys Ser Asp Gly 
    690                 695                 700                 


Phe Ala Asn Arg Asn Phe Met Gln Leu Ile His Asp Asp Ser Leu Thr 
705                 710                 715                 720 


Phe Lys Glu Asp Ile Gln Lys Ala Gln Val Ser Gly Gln Gly Asp Ser 
                725                 730                 735     


Leu His Glu His Ile Ala Asn Leu Ala Gly Ser Pro Ala Ile Lys Lys 
            740                 745                 750         


Gly Ile Leu Gln Thr Val Lys Val Val Asp Glu Leu Val Lys Val Met 
        755                 760                 765             


Gly Arg His Lys Pro Glu Asn Ile Val Ile Glu Met Ala Arg Glu Asn 
    770                 775                 780                 


Gln Thr Thr Gln Lys Gly Gln Lys Asn Ser Arg Glu Arg Met Lys Arg 
785                 790                 795                 800 


Ile Glu Glu Gly Ile Lys Glu Leu Gly Ser Gln Ile Leu Lys Glu His 
                805                 810                 815     


Pro Val Glu Asn Thr Gln Leu Gln Asn Glu Lys Leu Tyr Leu Tyr Tyr 
            820                 825                 830         


Leu Gln Asn Gly Arg Asp Met Tyr Val Asp Gln Glu Leu Asp Ile Asn 
        835                 840                 845             


Arg Leu Ser Asp Tyr Asp Val Asp His Ile Val Pro Gln Ser Phe Leu 
    850                 855                 860                 


Lys Asp Asp Ser Ile Asp Asn Lys Val Leu Thr Arg Ser Asp Lys Asn 
865                 870                 875                 880 


Arg Gly Lys Ser Asp Asn Val Pro Ser Glu Glu Val Val Lys Lys Met 
                885                 890                 895     


Lys Asn Tyr Trp Arg Gln Leu Leu Asn Ala Lys Leu Ile Thr Gln Arg 
            900                 905                 910         


Lys Phe Asp Asn Leu Thr Lys Ala Glu Arg Gly Gly Leu Ser Glu Leu 
        915                 920                 925             


Asp Lys Ala Gly Phe Ile Lys Arg Gln Leu Val Glu Thr Arg Gln Ile 
    930                 935                 940                 


Thr Lys His Val Ala Gln Ile Leu Asp Ser Arg Met Asn Thr Lys Tyr 
945                 950                 955                 960 


Asp Glu Asn Asp Lys Leu Ile Arg Glu Val Lys Val Ile Thr Leu Lys 
                965                 970                 975     


Ser Lys Leu Val Ser Asp Phe Arg Lys Asp Phe Gln Phe Tyr Lys Val 
            980                 985                 990         


Arg Glu Ile Asn Asn Tyr His His  Ala His Asp Ala Tyr  Leu Asn Ala 
        995                 1000                 1005             


Val Val  Gly Thr Ala Leu Ile  Lys Lys Tyr Pro Lys  Leu Glu Ser 
    1010                 1015                 1020             


Glu Phe  Val Tyr Gly Asp Tyr  Lys Val Tyr Asp Val  Arg Lys Met 
    1025                 1030                 1035             


Ile Ala  Lys Ser Glu Gln Glu  Ile Gly Lys Ala Thr  Ala Lys Tyr 
    1040                 1045                 1050             


Phe Phe  Tyr Ser Asn Ile Met  Asn Phe Phe Lys Thr  Glu Ile Thr 
    1055                 1060                 1065             


Leu Ala  Asn Gly Glu Ile Arg  Lys Arg Pro Leu Ile  Glu Thr Asn 
    1070                 1075                 1080             


Gly Glu  Thr Gly Glu Ile Val  Trp Asp Lys Gly Arg  Asp Phe Ala 
    1085                 1090                 1095             


Thr Val  Arg Lys Val Leu Ser  Met Pro Gln Val Asn  Ile Val Lys 
    1100                 1105                 1110             


Lys Thr  Glu Val Gln Thr Gly  Gly Phe Ser Lys Glu  Ser Ile Leu 
    1115                 1120                 1125             


Pro Lys  Arg Asn Ser Asp Lys  Leu Ile Ala Arg Lys  Lys Asp Trp 
    1130                 1135                 1140             


Asp Pro  Lys Lys Tyr Gly Gly  Phe Asp Ser Pro Thr  Val Ala Tyr 
    1145                 1150                 1155             


Ser Val  Leu Val Val Ala Lys  Val Glu Lys Gly Lys  Ser Lys Lys 
    1160                 1165                 1170             


Leu Lys  Ser Val Lys Glu Leu  Leu Gly Ile Thr Ile  Met Glu Arg 
    1175                 1180                 1185             


Ser Ser  Phe Glu Lys Asn Pro  Ile Asp Phe Leu Glu  Ala Lys Gly 
    1190                 1195                 1200             


Tyr Lys  Glu Val Lys Lys Asp  Leu Ile Ile Lys Leu  Pro Lys Tyr 
    1205                 1210                 1215             


Ser Leu  Phe Glu Leu Glu Asn  Gly Arg Lys Arg Met  Leu Ala Ser 
    1220                 1225                 1230             


Ala Gly  Glu Leu Gln Lys Gly  Asn Glu Leu Ala Leu  Pro Ser Lys 
    1235                 1240                 1245             


Tyr Val  Asn Phe Leu Tyr Leu  Ala Ser His Tyr Glu  Lys Leu Lys 
    1250                 1255                 1260             


Gly Ser  Pro Glu Asp Asn Glu  Gln Lys Gln Leu Phe  Val Glu Gln 
    1265                 1270                 1275             


His Lys  His Tyr Leu Asp Glu  Ile Ile Glu Gln Ile  Ser Glu Phe 
    1280                 1285                 1290             


Ser Lys  Arg Val Ile Leu Ala  Asp Ala Asn Leu Asp  Lys Val Leu 
    1295                 1300                 1305             


Ser Ala  Tyr Asn Lys His Arg  Asp Lys Pro Ile Arg  Glu Gln Ala 
    1310                 1315                 1320             


Glu Asn  Ile Ile His Leu Phe  Thr Leu Thr Asn Leu  Gly Ala Pro 
    1325                 1330                 1335             


Ala Ala  Phe Lys Tyr Phe Asp  Thr Thr Ile Asp Arg  Lys Arg Tyr 
    1340                 1345                 1350             


Thr Ser  Thr Lys Glu Val Leu  Asp Ala Thr Leu Ile  His Gln Ser 
    1355                 1360                 1365             


Ile Thr  Gly Leu Tyr Glu Thr  Arg Ile Asp Leu Ser  Gln Leu Gly 
    1370                 1375                 1380             


Gly Asp  Lys Arg Pro Ala Ala  Thr Lys Lys Ala Gly  Gln Ala Lys 
    1385                 1390                 1395             


Lys Lys  Lys 
    1400     


