                         SEQUENCE LISTING

<110>  UNIVERSITY OF MIAMI
 
<120>  DOUBLE SUICIDE GENE VECTOR SYSTEMS FOR STEM CELLS

<130>  32286/51087A

<150>  US 62/394,977
<151>  2016-09-15

<160>  44    

<170>  PatentIn version 3.5

<210>  1
<211>  6560
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic polynucleotide


<220>
<221>  misc_feature
<223>  pUC57LoxNRNeoRLox

<400>  1
tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca       60

cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg      120

ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc      180

accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc      240

attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat      300

tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt      360

tttcccagtc acgacgttgt aaaacgacgg ccagtgaatt cctagagacg gatcgggaga      420

tctcccgatc ccctatggtg cactctcagt acaatctgct ctgatgccgc atagttaagc      480

cagtatctgc tccctgcttg tgtgttggag gtcgctgagt agtgcgcgag caaaatttaa      540

gctacaacaa ggcaaggctt gaccgacaat tgcatgaaga atctgcttag ggttaggcgt      600

tttgcgctgc ttcgcgaggt aaccataact tcgtatagca tacattatac gaagttattg      660

tacgggccag atatacgcgt tgacattgat tattgactag ttattaatag taatcaatta      720

cggggtcatt agttcatagc ccatatatgg agttccgcgt tacataactt acggtaaatg      780

gcccgcctgg ctgaccgccc aacgaccccc gcccattgac gtcaataatg acgtatgttc      840

ccatagtaac gccaataggg actttccatt gacgtcaatg ggtggagtat ttacggtaaa      900

ctgcccactt ggcagtacat caagtgtatc atatgccaag tacgccccct attgacgtca      960

atgacggtaa atggcccgcc tggcattatg cccagtacat gaccttatgg gactttccta     1020

cttggcagta catctacgta ttagtcatcg ctattaccat ggtgatgcgg ttttggcagt     1080

acatcaatgg gcgtggatag cggtttgact cacggggatt tccaagtctc caccccattg     1140

acgtcaatgg gagtttgttt tggcaccaaa atcaacggga ctttccaaaa tgtcgtaaca     1200

actccgcccc attgacgcaa atgggcggta ggcgtgtacg gtgggaggtc tatataagca     1260

gagctctctg gctaactaga gaacccactg cttactggct tatcgaaatt aatacgactc     1320

actataggga gacccaagct ggctagcgtt taaacttaag cttggtacgc caccatggac     1380

atcatttcag tggcactgaa gcgacacagc actaaagcat tcgatgcctc caagaaactg     1440

actcccgagc aggctgaaca gatcaagacc ctgctgcagt actctccaag ctccctgaac     1500

agtcagccct ggcactttat tgtggcctct acagaggaag gaaaggctag ggtggcaaaa     1560

tctgccgctg gcaattacgt gttcagcgag cgcaagatgc tggacgcctc acatgtggtc     1620

gtgttttgcg ccaagaccgc tatggacgac gtgtggctga aactggtcgt ggaccaggag     1680

gacgccgatg ggagattcgc aacacctgaa gccaaagcag ccaacgataa gggacggaaa     1740

ttctttgccg acatgcacag aaaggatctg catgacgatg ctgagtggat ggcaaaacag     1800

gtgtacctga acgtcggcaa ttttctgctg ggagtggctg cactgggact ggacgccgtc     1860

cctatcgaag gcttcgacgc cgctattctg gatgctgagt ttggcctgaa ggaaaaaggg     1920

tatacaagcc tggtcgtggt cccagtgggg caccattccg tcgaggattt caacgccact     1980

ctgcccaagt cccggctgcc tcagaatatc accctgacag aagtgtgatg accgctgatc     2040

agcctttaat taacgactgt gccttctagt tgccagccat ctgttgtttg cccctccccc     2100

gtgccttcct tgaccctgga aggtgccact cccactgtcc tttcctaata aaatgaggaa     2160

attgcatcgc attgtctgag taggtgtcat tctattctgg ggggtggggt ggggcaggac     2220

agcaaggggg aggattggga agacaatagc aggcatgctg gggatgcggt gggctctatg     2280

gcttctgagg cggaaagaac cagctggggc tctagggggt atccccacgc gccctgtagc     2340

ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac acttgccagc     2400

gccctagcgc ccgctccttt cgctttcttc ccttcctttc tcgccacgtt cgccggcttt     2460

ccccgtcaag ctctaaatcg ggggctccct ttagggttcc gatttagtgc tttacggcac     2520

ctcgacccca aaaaacttga ttagggtgat ggttcacgta gtgggccatc gccctgatag     2580

acggtttttc gccctttgac gttggagtcc acgttcttta atagtggact cttgttccaa     2640

actggaacaa cactcaaccc tatctcggtc tattcttttg atttataagg gattttgccg     2700

atttcggcct attggttaaa aaatgagctg atttaacaaa aatttaacgc gaattaattc     2760

tgtggaatgt gtgtcagtta gggtgtggaa agtccccagg ctccccagca ggcagaagta     2820

tgcaaagcat gcatctcaat tagtcagcaa ccaggtgtgg aaagtcccca ggctccccag     2880

caggcagaag tatgcaaagc atgcatctca attagtcagc aaccatagtc ccgcccctaa     2940

ctccgcccat cccgccccta actccgccca gttccgccca ttctccgccc catggctgac     3000

taattttttt tatttatgca gaggccgagg ccgcctctgc ctctgagcta ttccagaagt     3060

agtgaggagg cttttttgga ggcctaggct tttgcaaaaa gctcccggga gcttgtatat     3120

ccattttcgg atctgatcaa gagacaggat gaggatcgtt tcgcatgatt gaacaagatg     3180

gattgcacgc aggttctccg gccgcttggg tggagaggct attcggctat gactgggcac     3240

aacagacaat cggctgctct gatgccgccg tgttccggct gtcagcgcag gggcgcccgg     3300

ttctttttgt caagaccgac ctgtccggtg ccctgaatga actgcaggac gaggcagcgc     3360

ggctatcgtg gctggccacg acgggcgttc cttgcgcagc tgtgctcgac gttgtcactg     3420

aagcgggaag ggactggctg ctattgggcg aagtgccggg gcaggatctc ctgtcatctc     3480

accttgctcc tgccgagaaa gtatccatca tggctgatgc aatgcggcgg ctgcatacgc     3540

ttgatccggc tacctgccca ttcgaccacc aagcgaaaca tcgcatcgag cgagcacgta     3600

ctcggatgga agccggtctt gtcgatcagg atgatctgga cgaagagcat caggggctcg     3660

cgccagccga actgttcgcc aggctcaagg cgcgcatgcc cgacggcgag gatctcgtcg     3720

tgacccatgg cgatgcctgc ttgccgaata tcatggtgga aaatggccgc ttttctggat     3780

tcatcgactg tggccggctg ggtgtggcgg accgctatca ggacatagcg ttggctaccc     3840

gtgatattgc tgaagagctt ggcggcgaat gggctgaccg cttcctcgtg ctttacggta     3900

tcgccgctcc cgattcgcag cgcatcgcct tctatcgcct tcttgacgag ttcttctgag     3960

cgggactctg gggttcgaaa tgaccgacca agcgacgccc aacctgccat cacgagattt     4020

cgattccacc gccgccttct atgaaaggtt gggcttcgga atcgttttcc gggacgccgg     4080

ctggatgatc ctccagcgcg gggatctcat gctggagttc ttcgcccacc ccaacttgtt     4140

tattgcagct tataatggtt acaaataaag caatagcatc acaaatttca caaataaagc     4200

atttttttca ctgcattcta gttgtggttt gtccaaactc atcaatgtat cttatataac     4260

ttcgtatagc atacattata cgaagttatg cggccgcgtc gactgcagag gcctgcatgc     4320

aagcttggcg taatcatggt catagctgtt tcctgtgtga aattgttatc cgctcacaat     4380

tccacacaac atacgagccg gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag     4440

ctaactcaca ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg     4500

ccagctgcat taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc     4560

ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc     4620

agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa     4680

catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt     4740

tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg     4800

gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg     4860

ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag     4920

cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc     4980

caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa     5040

ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg     5100

taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc     5160

taactacggc tacactagaa gaacagtatt tggtatctgc gctctgctga agccagttac     5220

cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg     5280

tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt     5340

gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt     5400

catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa     5460

atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga     5520

ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt     5580

gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg     5640

agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga     5700

gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga     5760

agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg     5820

catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc     5880

aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc     5940

gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca     6000

taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac     6060

caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg     6120

ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc     6180

ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg     6240

tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac     6300

aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat     6360

actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata     6420

catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa     6480

agtgccacct gacgtctaag aaaccattat tatcatgaca ttaacctata aaaataggcg     6540

tatcacgagg ccctttcgtc                                                 6560


<210>  2
<211>  7862
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic polynucleotide


<220>
<221>  misc_feature
<223>  pPURHtertpTKHipCre

<400>  2
tggaatgtgt gtcagttagg gtgtggaaag tccccaggct ccccagcagg cagaagtatg       60

caaagcatgc atctcaatta gtcagcaacc aggtgtggaa agtccccagg ctccccagca      120

ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa ccatagtccc gcccctaact      180

ccgcccatcc cgcccctaac tccgcccagt tccgcccatt ctccgcccca tggctgacta      240

atttttttta tttatgcaga ggccgaggcc gcctcggcct ctgagctatt ccagaagtag      300

tgaggaggct tttttggagg cctaggcttt tgcaaaaagc ttgcatgcct gcaggtcggc      360

cgccacgacc ggtgccgcca ccatcccctg acccacgccc ctgacccctc acaaggagac      420

gaccttccat gaccgagtac aagcccacgg tgcgcctcgc cacccgcgac gacgtccccc      480

gggccgtacg caccctcgcc gccgcgttcg ccgactaccc cgccacgcgc cacaccgtcg      540

acccggaccg ccacatcgag cgggtcaccg agctgcaaga actcttcctc acgcgcgtcg      600

ggctcgacat cggcaaggtg tgggtcgcgg acgacggcgc cgcggtggcg gtctggacca      660

cgccggagag cgtcgaagcg ggggcggtgt tcgccgagat cggcccgcgc atggccgagt      720

tgagcggttc ccggctggcc gcgcagcaac agatggaagg cctcctggcg ccgcaccggc      780

ccaaggagcc cgcgtggttc ctggccaccg tcggcgtctc gcccgaccac cagggcaagg      840

gtctgggcag cgccgtcgtg ctccccggag tggaggcggc cgagcgcgcc ggggtgcccg      900

ccttcctgga gacctccgcg ccccgcaacc tccccttcta cgagcggctc ggcttcaccg      960

tcaccgccga cgtcgagtgc ccgaaggacc gcgcgacctg gtgcatgacc cgcaagcccg     1020

gtgcctgacg cccgccccac gacccgcagc gcccgaccga aaggagcgca cgaccccatg     1080

gctccgaccg aagccgaccc gggcggcccc gccgaccccg cacccgcccc cgaggcccac     1140

cgactctaga ggatcataat cagccatacc acatttgtag aggttttact tgctttaaaa     1200

aacctcccac acctccccct gaacctgaaa cataaaatga atgcaattgt tgttgttaac     1260

ttgtttattg cagcttataa tggttacaaa taaagcaata gcatcacaaa tttcacaaat     1320

aaagcatttt tttcactgca ttctagttgt ggtttgtcca aactcatcaa tgtatcttat     1380

catgtctgga tccccaggaa gctcctctgt gtcctcataa accctaacct cctctacttg     1440

agaggacatt ccaatcatag gctgcccatc caccctctgt gtcctcctgt taattaggtc     1500

acttaacaaa aaggaaattg ggtaggggtt tttcacagac cgctttctaa gggtaatttt     1560

aaaatatctg ggaagtccct tccactgctg tgttccagaa gtgttggtaa acagcccaca     1620

aatgtcaaca gcagaaacat acaagctgtc agctttgcac aagggcccaa caccctgctc     1680

atcaagaagc actgtggttg ctgtgttagt aatgtgcaaa acaggaggca cattttcccc     1740

acctgtgtag gttccaaaat atctagtgtt ttcattttta cttggatcag gaacccagca     1800

ctccactgga taagcattat ccttatccaa aacagccttg tggtcagtgt tcatctgctg     1860

actgtcaact gtagcatttt ttggggttac agtttgagca ggatatttgg tcctgtagtt     1920

tgctaacaca ccctgcagct ccaaaggttc cccaccaaca gcaaaaaaat gaaaatttga     1980

cccttgaatg ggttttccag caccattttc atgagttttt tgtgtccctg aatgcaagtt     2040

taacatagca gttaccccaa taacctcagt tttaacagta acagcttccc acatcaaaat     2100

atttccacag gttaagtcct catttaaatt aggcaaagga attcttgaag acgaaagggc     2160

ctcgtgatac gcctattttt ataggttaat gtcatgataa taatggtttc ttagacgtca     2220

ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat     2280

tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa     2340

aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt     2400

tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag     2460

ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt     2520

tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg     2580

gtattatccc gtgttgacgc cgggcaagag caactcggtc gccgcataca ctattctcag     2640

aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta     2700

agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg     2760

acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta     2820

actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac     2880

accacgatgc ctgcagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt     2940

actctagctt cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca     3000

cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag     3060

cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta     3120

gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag     3180

ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt     3240

tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat     3300

aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta     3360

gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa     3420

acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt     3480

tttccgaagg taactggctt cagcagagcg cagataccaa atactgtcct tctagtgtag     3540

ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta     3600

atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca     3660

agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag     3720

cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa     3780

agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga     3840

acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc     3900

gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc     3960

ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt     4020

gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt     4080

gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag     4140

gaagcggaag agcgcctgat gcggtatttt ctccttacgc atctgtgcgg tatttcacac     4200

cgcatatggt gcactctcag tacaatctgc tctgatgccg catagttcag ctgagtggat     4260

tcgcgggcac agacgcccag gaccgcgctt cccacgtggc ggagggactg gggacccggg     4320

cacccgtcct gccccttcac cttccagctc cgcctcctcc gcgcggaccc cgccccgtcc     4380

cgacccctcc cgggtccccg gcccagcccc ctccgggccc tcccagcccc tccccttcct     4440

ttccgcggcc ccgccctctc ctcgcggcgc gagtttcagg cagcgctgcg tcctgctgcg     4500

cacgtgggaa gccctggccc cggccacccc cgcgggccgg cccgccacca tggcctccta     4560

cccatgccac cagcatgcat ctgccttcga ccaggccgct agatcaaggg gccacagcaa     4620

ccggagaacc gccctgaggc ccaggcgcca gcaggaggct accgaagtgc gccctgagca     4680

gaagatgcca acactgctgc gagtctatat cgatggccct catgggatgg gaaaaaccac     4740

aactacccag ctgctggtgg ccctggggtc ccgggacgac atcgtgtacg tccctgagcc     4800

aatgacttat tggagagtgc tgggggcctc tgaaaccatc gctaatatct acacaactca     4860

gcaccgcctg gaccagggag agatcagcgc cggcgatgca gccgtggtca tgacctccgc     4920

acagattaca atgggaatgc cttatgccgt gaccgacgcc gtcctggctc cacacatcgg     4980

aggagaagca ggcagctccc atgcaccacc tccagccctg acaatcttcc tggataggca     5040

cccaattgct tttatgctgt gctaccccgc tgcacgctat ctgatgggca gcatgacccc     5100

acaggcagtg ctggcctttg tcgctctgat cccccctaca ctgcccggga ctaacattgt     5160

gctgggagct ctgcctgagg acaggcatat cgatcgactg gcaaagcgac agcgaccagg     5220

agaacggctg gacctggcaa tgctggccgc tattcgacgg gtgtacggac tgctggctaa     5280

caccgtgaga tacctgcagt gtggaggcag ttggagggag gactggggac agctgtcagg     5340

gactgccgtg ccaccacagg gagctgaacc tcagagtaat gcaggccccc ggcctcacat     5400

cggggatact ctgttcaccc tgtttagagc acccgagctg ctggccccta acggagacct     5460

gtacaacgtg ttcgcttggg cactggatgt cctggccaaa cggctgcgga gcatgcacgt     5520

gtttattctg gactatgatc agagtcccgc tggctgtagg gatgcactgc tgcagctgac     5580

ttcaggaatg gtgcagaccc atgtcaccac accaggcagc atccccacaa tttgcgacct     5640

ggctcgaact ttcgcacggt ctagtgattg tgccttttga ttgccagcca tctgttgttt     5700

gcccctcccc cgtgccttcc ttgaccctgg aaggtgccac tcccactgtc ctttcctaat     5760

aaaatgagga aattgcatcg cattgtctga gtaggtgtca ttctattctg gggggtgggg     5820

tggggcagga cagcaagggg gaggattggg aagacaatag caggcatgct ggggatgcgg     5880

tgggctctat ggctctgtat accgtcgacc tctagctaga gctggcgcgc cgggacaggg     5940

gtgtggggac aggggtctgg ggacaggggt gtggggacag gggtcctggg gacaggggtg     6000

tggggatagg ggtgtgggga caggggtgtg gggacagggg tgtggggaca ggggtctggg     6060

gacagcagcg caaagagccc cgccctgcag cctccagctc tcctggtcta atgtggaaag     6120

tggcccaggt gagggctttg ctctcctgga gacatttgcc cccagctgtg agcagggaca     6180

ggtctggcca ccgggcccct ggttaagact ctaatgaccc gctggtcctg aggaagaggt     6240

gctgacgacc aaggagatct tcccacagac ccagcaccag ggaaatggtc cggaggcaaa     6300

gaattctgag ccgccaccat ggccaattta ctgaccgtgc accagaatct gccagctctg     6360

cccgtggacg caacatctga tgaggtccgg aagaacctga tggacatgtt ccgagatcgg     6420

caggccttta gtgaacatac ctggaaaatg ctgctgtcag tgtgccgaag ctgggccgct     6480

tggtgtaagc tgaacaatag aaaatggttc cctgccgagc cagaagacgt gcgcgattac     6540

ctgctgtatc tgcaggcccg agggctggct gtcaagacaa ttcagcagca cctggggcag     6600

ctgaatatgc tgcatcggag aagcggactg cccaggccta gcgactccaa tgccgtgtcc     6660

ctggtcatga ggcgcatccg gaaggagaac gtggatgcag gcgaaagagc caaacaggcc     6720

ctggctttcg agagaactga ctttgatcag gtgaggtccc tgatggaaaa ctctgacagg     6780

tgccaggata ttcgcaatct ggcctttctg ggaatcgctt acaacacact gctgcgcatc     6840

gctgagattg caagaatcag ggtgaaggac atttcccgaa ctgatggcgg cagaatgctg     6900

atccacattg gccggaccaa aacactggtg agtacagcag gagtcgagaa ggccctgtca     6960

ctgggcgtga ctaaactggt cgaaagatgg atctctgtga gtggcgtcgc agacgatccc     7020

aacaattatc tgttctgtcg cgtgcgaaag aacggggtcg cagccccttc agctactagc     7080

cagctgtcca ccagggccct ggagggaatt tttgaagcta cacaccgcct gatctacggg     7140

gccaaagacg attctggaca gaggtatctg gcttggtctg gacatagtgc acgcgtggga     7200

gctgcacggg acatggcaag agccggcgtc agcattcccg agatcatgca ggccggaggc     7260

tggactaacg tgaatattgt catgaactac atcagaaatc tggatagcga aaccggggct     7320

atggtgcgcc tgctggaaga tggcgatgga ccggtggaac aaaaacttat ttctgaagaa     7380

gatctgtgat acctcaggct aagcgtgcag gctgcctatc agaaggtggt ggctggtgtg     7440

gccaatgccc tggctcacaa ataccactga gatctttttc cctctgccaa aaattatggg     7500

gacatcatga agccccttga gcatctgact tctggctaat aaaggaaatt tattttcatt     7560

gcaatagtgt gttggaattt tttgtgtctc tcactcggaa ggacatatgg gagggcaaat     7620

catttaaaac atcagaatga gtatttggtt tagagtttgg caacatatgc catatgctgg     7680

ctgccatgaa caaaggtggc tataaagagg tcatcagtat atgaaacagc cccctgctgt     7740

ccattcctta ttccatagaa aagccttgac ttgaggttag atttttttta tattttgttt     7800

tgtgttattt ttttctttaa catccctaaa attttcctta catgttttac tagccacagc     7860

tg                                                                    7862


<210>  3
<211>  17
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic polynucleotide


<220>
<221>  misc_feature
<223>  M13 Fwd of SEQ ID NO: 1 (pUC57LoxNRNeoRLox)

<400>  3
gtaaaacgac ggccagt                                                      17


<210>  4
<211>  34
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic polynucleotide


<220>
<221>  misc_feature
<223>  LoxP of SEQ ID NO: 1 (pUC57LoxNRNeoRLox)

<400>  4
ataacttcgt atagcataca ttatacgaag ttat                                   34


<210>  5
<211>  380
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic polynucleotide


<220>
<221>  misc_feature
<223>  CMV Enhancer of SEQ ID NO: 1 (pUC57LoxNRNeoRLox)

<400>  5
gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc       60

catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca      120

acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga      180

ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc      240

aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct      300

ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat      360

tagtcatcgc tattaccatg                                                  380


<210>  6
<211>  204
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic polynucleotide


<220>
<221>  misc_feature
<223>  CMV promoter of SEQ ID NO: 1 (pUC57LoxNRNeoRLox)

<400>  6
gtgatgcggt tttggcagta catcaatggg cgtggatagc ggtttgactc acggggattt       60

ccaagtctcc accccattga cgtcaatggg agtttgtttt ggcaccaaaa tcaacgggac      120

tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa tgggcggtag gcgtgtacgg      180

tgggaggtct atataagcag agct                                             204


<210>  7
<211>  19
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic polynucleotide


<220>
<221>  misc_feature
<223>  T7 Promoter of SEQ ID NO: 1 (pUC57LoxNRNeoRLox)

<400>  7
taatacgact cactatagg                                                    19


<210>  8
<211>  509
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic polynucleotide


<220>
<221>  misc_feature
<223>  Codon Optimized Nitroreductase for efficient mammalian 
       translation of SEQ ID NO: 1 (pUC57LoxNRNeoRLox)

<400>  8
actaaagcat tcgatgcctc caagaaactg actcccgagc aggctgaaca gatcaagacc       60

ctgctgcagt actctccaag ctccctgaac agtcagccct ggcactttat tgtggcctct      120

acagaggaag gaaaggctag ggtggcaaaa tctgccgctg gcaattacgt gttcagcgag      180

cgcaagatgc tggacgcctc acatgtggtc gtgttttgcg ccaagaccgc tatggacgac      240

gtgtggctga aactggtcgt ggaccaggag gacgccgatg ggagattcgc aacacctgaa      300

gccaaagcag ccaacgataa gggacggaaa ttctttgccg acatgcacag aaaggatctg      360

catgacgatg ctgagtggat ggcaaaacag gtgtacctga acgtcggcaa ttttctgctg      420

ggagtggctg cactgggact ggacgccgtc cctatcgaag gcttcgacgc cgctattctg      480

gatgctgagt ttggcctgaa ggaaaaagg                                        509


<210>  9
<211>  225
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic polynucleotide


<220>
<221>  misc_feature
<223>  bGH poly(A) signal of SEQ ID NO: 1 (pUC57LoxNRNeoRLox)

<400>  9
ctgtgccttc tagttgccag ccatctgttg tttgcccctc ccccgtgcct tccttgaccc       60

tggaaggtgc cactcccact gtcctttcct aataaaatga ggaaattgca tcgcattgtc      120

tgagtaggtg tcattctatt ctggggggtg gggtggggca ggacagcaag ggggaggatt      180

gggaagacaa tagcaggcat gctggggatg cggtgggctc tatgg                      225


<210>  10
<211>  429
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic polynucleotide


<220>
<221>  misc_feature
<223>  f1 ori of SEQ ID NO: 1 (pUC57LoxNRNeoRLox)

<400>  10
acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg       60

ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca      120

cgttcgccgg ctttccccgt caagctctaa atcgggggct ccctttaggg ttccgattta      180

gtgctttacg gcacctcgac cccaaaaaac ttgattaggg tgatggttca cgtagtgggc      240

catcgccctg atagacggtt tttcgccctt tgacgttgga gtccacgttc tttaatagtg      300

gactcttgtt ccaaactgga acaacactca accctatctc ggtctattct tttgatttat      360

aagggatttt gccgatttcg gcctattggt taaaaaatga gctgatttaa caaaaattta      420

acgcgaatt                                                              429


<210>  11
<211>  330
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic polynucleotide


<220>
<221>  misc_feature
<223>  SV40 promoter of SEQ ID NO: 1 (pUC57LoxNRNeoRLox)

<400>  11
gtgtgtcagt tagggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc       60

atgcatctca attagtcagc aaccaggtgt ggaaagtccc caggctcccc agcaggcaga      120

agtatgcaaa gcatgcatct caattagtca gcaaccatag tcccgcccct aactccgccc      180

atcccgcccc taactccgcc cagttccgcc cattctccgc cccatggctg actaattttt      240

tttatttatg cagaggccga ggccgcctct gcctctgagc tattccagaa gtagtgagga      300

ggcttttttg gaggcctagg cttttgcaaa                                       330


<210>  12
<211>  795
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic polynucleotide


<220>
<221>  misc_feature
<223>  Neomycin resistance gene Aminoglycoside phosphotransferase from 
       Tn5 of SEQ ID NO: 1 (pUC57LoxNRNeoRLox)

<400>  12
atgattgaac aagatggatt gcacgcaggt tctccggccg cttgggtgga gaggctattc       60

ggctatgact gggcacaaca gacaatcggc tgctctgatg ccgccgtgtt ccggctgtca      120

gcgcaggggc gcccggttct ttttgtcaag accgacctgt ccggtgccct gaatgaactg      180

caggacgagg cagcgcggct atcgtggctg gccacgacgg gcgttccttg cgcagctgtg      240

ctcgacgttg tcactgaagc gggaagggac tggctgctat tgggcgaagt gccggggcag      300

gatctcctgt catctcacct tgctcctgcc gagaaagtat ccatcatggc tgatgcaatg      360

cggcggctgc atacgcttga tccggctacc tgcccattcg accaccaagc gaaacatcgc      420

atcgagcgag cacgtactcg gatggaagcc ggtcttgtcg atcaggatga tctggacgaa      480

gagcatcagg ggctcgcgcc agccgaactg ttcgccaggc tcaaggcgcg catgcccgac      540

ggcgaggatc tcgtcgtgac ccatggcgat gcctgcttgc cgaatatcat ggtggaaaat      600

ggccgctttt ctggattcat cgactgtggc cggctgggtg tggcggaccg ctatcaggac      660

atagcgttgg ctacccgtga tattgctgaa gagcttggcg gcgaatgggc tgaccgcttc      720

ctcgtgcttt acggtatcgc cgctcccgat tcgcagcgca tcgccttcta tcgccttctt      780

gacgagttct tctga                                                       795


<210>  13
<211>  122
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic polynucleotide


<220>
<221>  misc_feature
<223>  SV40 poly(A) signal of SEQ ID NO: 1 (pUC57LoxNRNeoRLox)

<400>  13
aacttgttta ttgcagctta taatggttac aaataaagca atagcatcac aaatttcaca       60

aataaagcat ttttttcact gcattctagt tgtggtttgt ccaaactcat caatgtatct      120

ta                                                                     122


<210>  14
<211>  34
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic polynucleotide


<220>
<221>  misc_feature
<223>  LoxP of SEQ ID NO: 1 (pUC57LoxNRNeoRLox)

<400>  14
ataacttcgt atagcataca ttatacgaag ttat                                   34


<210>  15
<211>  17
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic polynucleotide


<220>
<221>  misc_feature
<223>  M13rev of SEQ ID NO: 1 (pUC57LoxNRNeoRLox)

<400>  15
gtcatagctg tttcctg                                                      17


<210>  16
<211>  17
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic polynucleotide


<220>
<221>  misc_feature
<223>  Lac operator of SEQ ID NO: 1 (pUC57LoxNRNeoRLox)

<400>  16
ttgttatccg ctcacaa                                                      17


<210>  17
<211>  31
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic polynucleotide


<220>
<221>  misc_feature
<223>  Lac promoter of SEQ ID NO: 1 (pUC57LoxNRNeoRLox)

<400>  17
caacatacga gccggaagca taaagtgtaa a                                      31


<210>  18
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic polynucleotide


<220>
<221>  misc_feature
<223>  CAP binding site of SEQ ID NO: 1 (pUC57LoxNRNeoRLox)

<400>  18
atgagtgagc taactcacat ta                                                22


<210>  19
<211>  589
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic polynucleotide


<220>
<221>  misc_feature
<223>  Ori seq of SEQ ID NO: 1 (pUC57LoxNRNeoRLox)

<400>  19
tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg       60

gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg      120

ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag      180

cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc      240

caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa      300

ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg      360

taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc      420

taactacggc tacactagaa gaacagtatt tggtatctgc gctctgctga agccagttac      480

cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg      540

tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaa                  589


<210>  20
<211>  861
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic polynucleotide


<220>
<221>  misc_feature
<223>  Ampicillin resistance gene Beta-lactamase of SEQ ID NO: 1 
       (pUC57LoxNRNeoRLox)

<400>  20
ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat       60

agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc      120

cagtgctgca atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa      180

ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca      240

gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa      300

cgttgttgcc attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt      360

cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc      420

ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact      480

catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc      540

tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg      600

ctcttgcccg gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct      660

catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc      720

cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag      780

cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac      840

acggaaatgt tgaatactca t                                                861


<210>  21
<211>  105
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic polynucleotide


<220>
<221>  misc_feature
<223>  Amp promoter of SEQ ID NO: 1 (pUC57LoxNRNeoRLox)

<400>  21
actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata       60

catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcg                      105


<210>  22
<211>  330
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic polynucleotide


<220>
<221>  misc_feature
<223>  SV40 Promoter of SEQ ID NO: 2 (pPURHtertpTKHipCre)

<400>  22
gtgtgtcagt tagggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc       60

atgcatctca attagtcagc aaccaggtgt ggaaagtccc caggctcccc agcaggcaga      120

agtatgcaaa gcatgcatct caattagtca gcaaccatag tcccgcccct aactccgccc      180

atcccgcccc taactccgcc cagttccgcc cattctccgc cccatggctg actaattttt      240

tttatttatg cagaggccga ggccgcctcg gcctctgagc tattccagaa gtagtgagga      300

ggcttttttg gaggcctagg cttttgcaaa                                       330


<210>  23
<211>  600
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic polynucleotide


<220>
<221>  misc_feature
<223>  Puromycin resistance gene Puromycin N-acetyl transferase of SEQ 
       ID NO: 2 (pPURHtertpTKHipCre)

<400>  23
atgaccgagt acaagcccac ggtgcgcctc gccacccgcg acgacgtccc ccgggccgta       60

cgcaccctcg ccgccgcgtt cgccgactac cccgccacgc gccacaccgt cgacccggac      120

cgccacatcg agcgggtcac cgagctgcaa gaactcttcc tcacgcgcgt cgggctcgac      180

atcggcaagg tgtgggtcgc ggacgacggc gccgcggtgg cggtctggac cacgccggag      240

agcgtcgaag cgggggcggt gttcgccgag atcggcccgc gcatggccga gttgagcggt      300

tcccggctgg ccgcgcagca acagatggaa ggcctcctgg cgccgcaccg gcccaaggag      360

cccgcgtggt tcctggccac cgtcggcgtc tcgcccgacc accagggcaa gggtctgggc      420

agcgccgtcg tgctccccgg agtggaggcg gccgagcgcg ccggggtgcc cgccttcctg      480

gagacctccg cgccccgcaa cctccccttc tacgagcggc tcggcttcac cgtcaccgcc      540

gacgtcgagt gcccgaagga ccgcgcgacc tggtgcatga cccgcaagcc cggtgcctga      600


<210>  24
<211>  135
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic polynucleotide


<220>
<221>  misc_feature
<223>  SV40 poly(A) signal of SEQ ID NO: 2 (pPURHtertpTKHipCre)

<400>  24
aacttgttta ttgcagctta taatggttac aaataaagca atagcatcac aaatttcaca       60

aataaagcat ttttttcact gcattctagt tgtggtttgt ccaaactcat caatgtatct      120

tatcatgtct ggatc                                                       135


<210>  25
<211>  105
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic polynucleotide


<220>
<221>  misc_feature
<223>  AmpR promoter of SEQ ID NO: 2 (pPURHtertpTKHipCre)

<400>  25
cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga       60

caataaccct gataaatgct tcaataatat tgaaaaagga agagt                      105


<210>  26
<211>  861
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic polynucleotide


<220>
<221>  misc_feature
<223>  Ampicillin resistance gene Beta lactamase of SEQ ID NO: 2 
       (pPURHtertpTKHipCre)

<400>  26
atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct       60

gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca      120

cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc      180

gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc      240

cgtgttgacg ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg      300

gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta      360

tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc      420

ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt      480

gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg      540

cctgcagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct      600

tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc      660

tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct      720

cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac      780

acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc      840

tcactgatta agcattggta a                                                861


<210>  27
<211>  589
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic polynucleotide


<220>
<221>  misc_feature
<223>  Ori seq of SEQ ID NO: 2 (pPURHtertpTKHipCre)

<400>  27
ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc       60

agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt      120

cagcagagcg cagataccaa atactgtcct tctagtgtag ccgtagttag gccaccactt      180

caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc      240

tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa      300

ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac      360

ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg      420

gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga      480

gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact      540

tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaa                  589


<210>  28
<211>  281
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic polynucleotide


<220>
<221>  misc_feature
<223>  hTERTp of SEQ ID NO: 2 (pPURHtertpTKHipCre)

<400>  28
agtggattcg cgggcacaga cgcccaggac cgcgcttccc acgtggcgga gggactgggg       60

acccgggcac ccgtcctgcc ccttcacctt ccagctccgc ctcctccgcg cggaccccgc      120

cccgtcccga cccctcccgg gtccccggcc cagccccctc cgggccctcc cagcccctcc      180

ccttcctttc cgcggccccg ccctctcctc gcggcgcgag tttcaggcag cgctgcgtcc      240

tgctgcgcac gtgggaagcc ctggccccgg ccacccccgc g                          281


<210>  29
<211>  1115
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic polynucleotide


<220>
<221>  misc_feature
<223>  Herpes Simplex Virus Thymidine Kinase gene codon optimized for 
       mammalian transalation of SEQ ID NO: 2 (pPURHtertpTKHipCre)

<400>  29
atggcctcct acccatgcca ccagcatgca tctgccttcg accaggccgc tagatcaagg       60

ggccacagca accggagaac cgccctgagg cccaggcgcc agcaggaggc taccgaagtg      120

cgccctgagc agaagatgcc aacactgctg cgagtctata tcgatggccc tcatgggatg      180

ggaaaaacca caactaccca gctgctggtg gccctggggt cccgggacga catcgtgtac      240

gtccctgagc caatgactta ttggagagtg ctgggggcct ctgaaaccat cgctaatatc      300

tacacaactc agcaccgcct ggaccaggga gagatcagcg ccggcgatgc agccgtggtc      360

atgacctccg cacagattac aatgggaatg ccttatgccg tgaccgacgc cgtcctggct      420

ccacacatcg gaggagaagc aggcagctcc catgcaccac ctccagccct gacaatcttc      480

ctggataggc acccaattgc ttttatgctg tgctaccccg ctgcacgcta tctgatgggc      540

agcatgaccc cacaggcagt gctggccttt gtcgctctga tcccccctac actgcccggg      600

actaacattg tgctgggagc tctgcctgag gacaggcata tcgatcgact ggcaaagcga      660

cagcgaccag gagaacggct ggacctggca atgctggccg ctattcgacg ggtgtacgga      720

ctgctggcta acaccgtgag atacctgcag tgtggaggca gttggaggga ggactgggga      780

cagctgtcag ggactgccgt gccaccacag ggagctgaac ctcagagtaa tgcaggcccc      840

cggcctcaca tcggggatac tctgttcacc ctgtttagag cacccgagct gctggcccct      900

aacggagacc tgtacaacgt gttcgcttgg gcactggatg tcctggccaa acggctgcgg      960

agcatgcacg tgtttattct ggactatgat cagagtcccg ctggctgtag ggatgcactg     1020

ctgcagctga cttcaggaat ggtgcagacc catgtcacca caccaggcag catccccaca     1080

atttgcgacc tggctcgaac tttcgcacgg tctag                                1115


<210>  30
<211>  232
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic polynucleotide


<220>
<221>  misc_feature
<223>  Bi-allelic poly(A) trap vector of SEQ ID NO: 2 
       (pPURHtertpTKHipCre)

<400>  30
gattgtgcct tttgattgcc agccatctgt tgtttgcccc tcccccgtgc cttccttgac       60

cctggaaggt gccactccca ctgtcctttc ctaataaaat gaggaaattg catcgcattg      120

tctgagtagg tgtcattcta ttctgggggg tggggtgggg caggacagca agggggagga      180

ttgggaagac aatagcaggc atgctgggga tgcggtgggc tctatggctc tg              232


<210>  31
<211>  266
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic polynucleotide


<220>
<221>  misc_feature
<223>  Human Insulin Promoter of SEQ ID NO: 2 (pPURHtertpTKHipCre)

<400>  31
gacaggggtg tggggacagg ggtctgggga cagcagcgca aagagccccg ccctgcagcc       60

tccagctctc ctggtctaat gtggaaagtg gcccaggtga gggctttgct ctcctggaga      120

catttgcccc cagctgtgag cagggacagg tctggccacc gggcccctgg ttaagactct      180

aatgacccgc tggtcctgag gaagaggtgc tgacgaccaa ggagatcttc ccacagaccc      240

agcaccaggg aaatggtccg gaggca                                           266


<210>  32
<211>  1028
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic polynucleotide


<220>
<221>  misc_feature
<223>  Cre recombinase of SEQ ID NO: 2 (pPURHtertpTKHipCre)

<400>  32
ccaatttact gaccgtgcac cagaatctgc cagctctgcc cgtggacgca acatctgatg       60

aggtccggaa gaacctgatg gacatgttcc gagatcggca ggcctttagt gaacatacct      120

ggaaaatgct gctgtcagtg tgccgaagct gggccgcttg gtgtaagctg aacaatagaa      180

aatggttccc tgccgagcca gaagacgtgc gcgattacct gctgtatctg caggcccgag      240

ggctggctgt caagacaatt cagcagcacc tggggcagct gaatatgctg catcggagaa      300

gcggactgcc caggcctagc gactccaatg ccgtgtccct ggtcatgagg cgcatccgga      360

aggagaacgt ggatgcaggc gaaagagcca aacaggccct ggctttcgag agaactgact      420

ttgatcaggt gaggtccctg atggaaaact ctgacaggtg ccaggatatt cgcaatctgg      480

cctttctggg aatcgcttac aacacactgc tgcgcatcgc tgagattgca agaatcaggg      540

tgaaggacat ttcccgaact gatggcggca gaatgctgat ccacattggc cggaccaaaa      600

cactggtgag tacagcagga gtcgagaagg ccctgtcact gggcgtgact aaactggtcg      660

aaagatggat ctctgtgagt ggcgtcgcag acgatcccaa caattatctg ttctgtcgcg      720

tgcgaaagaa cggggtcgca gccccttcag ctactagcca gctgtccacc agggccctgg      780

agggaatttt tgaagctaca caccgcctga tctacggggc caaagacgat tctggacaga      840

ggtatctggc ttggtctgga catagtgcac gcgtgggagc tgcacgggac atggcaagag      900

ccggcgtcag cattcccgag atcatgcagg ccggaggctg gactaacgtg aatattgtca      960

tgaactacat cagaaatctg gatagcgaaa ccggggctat ggtgcgcctg ctggaagatg     1020

gcgatgga                                                              1028


<210>  33
<211>  30
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic polynucleotide


<220>
<221>  misc_feature
<223>  Myc of SEQ ID NO: 2 (pPURHtertpTKHipCre)

<400>  33
gaacaaaaac ttatttctga agaagatctg                                        30


<210>  34
<211>  56
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic polynucleotide


<220>
<221>  misc_feature
<223>  Beta-globin poly(A) signal of SEQ ID NO: 2 (pPURHtertpTKHipCre)

<400>  34
aataaaggaa atttattttc attgcaatag tgtgttggaa ttttttgtgt ctctca           56


<210>  35
<211>  16
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic polynucleotide


<220>
<221>  misc_feature
<223>  NTR forward primer

<400>  35
ccccacaggc agtgct                                                       16


<210>  36
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic polynucleotide


<220>
<221>  misc_feature
<223>  NTR reverse primer

<400>  36
gttagtcccg ggcagtgtag                                                   20


<210>  37
<211>  16
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic polynucleotide


<220>
<221>  misc_feature
<223>  NTR FAM probe

<400>  37
ttgtcgctct gatccc                                                       16


<210>  38
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic polynucleotide


<220>
<221>  misc_feature
<223>  HSV-TK forward primer

<400>  38
ccaaagcagc caacgataag g                                                 21


<210>  39
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic polynucleotide


<220>
<221>  misc_feature
<223>  HSV-TK reverse primer

<400>  39
ccatccactc agcatcgtca tg                                                22


<210>  40
<211>  15
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic polynucleotide


<220>
<221>  misc_feature
<223>  HSV-TK FAM probe

<400>  40
tcggcaaaga atttc                                                        15


<210>  41
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic polynucleotide


<220>
<221>  misc_feature
<223>  HSV-TK forward primer

<400>  41
cccgggacta acattgtgct                                                   20


<210>  42
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic polynucleotide


<220>
<221>  misc_feature
<223>  HSV-TK reverse primer

<400>  42
cagctcgggt gctctaaaca                                                   20


<210>  43
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic polynucleotide


<220>
<221>  misc_feature
<223>  NTR forward primer

<400>  43
cctcacatgt ggtcgtgttt                                                   20


<210>  44
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic polynucleotide


<220>
<221>  misc_feature
<223>  NTR reverse primer

<400>  44
acctgttttg ccatccactc                                                   20


