                         SEQUENCE LISTING

<110>  Janssen Vaccines & Prevention B.V.
       Rutten, Lucy
       Truan, Daphn
       Strokappe, Nika M.
       Langedijk, Johannes P.M.
 
<120>  Trimer Stabilizing HIV Envelope Protein Mutations

<130>  0276 WO 00 ORD

<150>  EP16188866.4
<151>  2016-09-15

<160>  34    

<170>  PatentIn version 3.5

<210>  1
<211>  856
<212>  PRT
<213>  HIV-1


<220>
<221>  misc_feature
<223>  gp160 of HIV-1 isolate HXB2

<400>  1

Met Arg Val Lys Glu Lys Tyr Gln His Leu Trp Arg Trp Gly Trp Arg 
1               5                   10                  15      


Trp Gly Thr Met Leu Leu Gly Met Leu Met Ile Cys Ser Ala Thr Glu 
            20                  25                  30          


Lys Leu Trp Val Thr Val Tyr Tyr Gly Val Pro Val Trp Lys Glu Ala 
        35                  40                  45              


Thr Thr Thr Leu Phe Cys Ala Ser Asp Ala Lys Ala Tyr Asp Thr Glu 
    50                  55                  60                  


Val His Asn Val Trp Ala Thr His Ala Cys Val Pro Thr Asp Pro Asn 
65                  70                  75                  80  


Pro Gln Glu Val Val Leu Val Asn Val Thr Glu Asn Phe Asn Met Trp 
                85                  90                  95      


Lys Asn Asp Met Val Glu Gln Met His Glu Asp Ile Ile Ser Leu Trp 
            100                 105                 110         


Asp Gln Ser Leu Lys Pro Cys Val Lys Leu Thr Pro Leu Cys Val Ser 
        115                 120                 125             


Leu Lys Cys Thr Asp Leu Lys Asn Asp Thr Asn Thr Asn Ser Ser Ser 
    130                 135                 140                 


Gly Arg Met Ile Met Glu Lys Gly Glu Ile Lys Asn Cys Ser Phe Asn 
145                 150                 155                 160 


Ile Ser Thr Ser Ile Arg Gly Lys Val Gln Lys Glu Tyr Ala Phe Phe 
                165                 170                 175     


Tyr Lys Leu Asp Ile Ile Pro Ile Asp Asn Asp Thr Thr Ser Tyr Lys 
            180                 185                 190         


Leu Thr Ser Cys Asn Thr Ser Val Ile Thr Gln Ala Cys Pro Lys Val 
        195                 200                 205             


Ser Phe Glu Pro Ile Pro Ile His Tyr Cys Ala Pro Ala Gly Phe Ala 
    210                 215                 220                 


Ile Leu Lys Cys Asn Asn Lys Thr Phe Asn Gly Thr Gly Pro Cys Thr 
225                 230                 235                 240 


Asn Val Ser Thr Val Gln Cys Thr His Gly Ile Arg Pro Val Val Ser 
                245                 250                 255     


Thr Gln Leu Leu Leu Asn Gly Ser Leu Ala Glu Glu Glu Val Val Ile 
            260                 265                 270         


Arg Ser Val Asn Phe Thr Asp Asn Ala Lys Thr Ile Ile Val Gln Leu 
        275                 280                 285             


Asn Thr Ser Val Glu Ile Asn Cys Thr Arg Pro Asn Asn Asn Thr Arg 
    290                 295                 300                 


Lys Arg Ile Arg Ile Gln Arg Gly Pro Gly Arg Ala Phe Val Thr Ile 
305                 310                 315                 320 


Gly Lys Ile Gly Asn Met Arg Gln Ala His Cys Asn Ile Ser Arg Ala 
                325                 330                 335     


Lys Trp Asn Asn Thr Leu Lys Gln Ile Ala Ser Lys Leu Arg Glu Gln 
            340                 345                 350         


Phe Gly Asn Asn Lys Thr Ile Ile Phe Lys Gln Ser Ser Gly Gly Asp 
        355                 360                 365             


Pro Glu Ile Val Thr His Ser Phe Asn Cys Gly Gly Glu Phe Phe Tyr 
    370                 375                 380                 


Cys Asn Ser Thr Gln Leu Phe Asn Ser Thr Trp Phe Asn Ser Thr Trp 
385                 390                 395                 400 


Ser Thr Glu Gly Ser Asn Asn Thr Glu Gly Ser Asp Thr Ile Thr Leu 
                405                 410                 415     


Pro Cys Arg Ile Lys Gln Ile Ile Asn Met Trp Gln Lys Val Gly Lys 
            420                 425                 430         


Ala Met Tyr Ala Pro Pro Ile Ser Gly Gln Ile Arg Cys Ser Ser Asn 
        435                 440                 445             


Ile Thr Gly Leu Leu Leu Thr Arg Asp Gly Gly Asn Ser Asn Asn Glu 
    450                 455                 460                 


Ser Glu Ile Phe Arg Pro Gly Gly Gly Asp Met Arg Asp Asn Trp Arg 
465                 470                 475                 480 


Ser Glu Leu Tyr Lys Tyr Lys Val Val Lys Ile Glu Pro Leu Gly Val 
                485                 490                 495     


Ala Pro Thr Lys Ala Lys Arg Arg Val Val Gln Arg Glu Lys Arg Ala 
            500                 505                 510         


Val Gly Ile Gly Ala Leu Phe Leu Gly Phe Leu Gly Ala Ala Gly Ser 
        515                 520                 525             


Thr Met Gly Ala Ala Ser Met Thr Leu Thr Val Gln Ala Arg Gln Leu 
    530                 535                 540                 


Leu Ser Gly Ile Val Gln Gln Gln Asn Asn Leu Leu Arg Ala Ile Glu 
545                 550                 555                 560 


Ala Gln Gln His Leu Leu Gln Leu Thr Val Trp Gly Ile Lys Gln Leu 
                565                 570                 575     


Gln Ala Arg Ile Leu Ala Val Glu Arg Tyr Leu Lys Asp Gln Gln Leu 
            580                 585                 590         


Leu Gly Ile Trp Gly Cys Ser Gly Lys Leu Ile Cys Thr Thr Ala Val 
        595                 600                 605             


Pro Trp Asn Ala Ser Trp Ser Asn Lys Ser Leu Glu Gln Ile Trp Asn 
    610                 615                 620                 


His Thr Thr Trp Met Glu Trp Asp Arg Glu Ile Asn Asn Tyr Thr Ser 
625                 630                 635                 640 


Leu Ile His Ser Leu Ile Glu Glu Ser Gln Asn Gln Gln Glu Lys Asn 
                645                 650                 655     


Glu Gln Glu Leu Leu Glu Leu Asp Lys Trp Ala Ser Leu Trp Asn Trp 
            660                 665                 670         


Phe Asn Ile Thr Asn Trp Leu Trp Tyr Ile Lys Leu Phe Ile Met Ile 
        675                 680                 685             


Val Gly Gly Leu Val Gly Leu Arg Ile Val Phe Ala Val Leu Ser Ile 
    690                 695                 700                 


Val Asn Arg Val Arg Gln Gly Tyr Ser Pro Leu Ser Phe Gln Thr His 
705                 710                 715                 720 


Leu Pro Thr Pro Arg Gly Pro Asp Arg Pro Glu Gly Ile Glu Glu Glu 
                725                 730                 735     


Gly Gly Glu Arg Asp Arg Asp Arg Ser Ile Arg Leu Val Asn Gly Ser 
            740                 745                 750         


Leu Ala Leu Ile Trp Asp Asp Leu Arg Ser Leu Cys Leu Phe Ser Tyr 
        755                 760                 765             


His Arg Leu Arg Asp Leu Leu Leu Ile Val Thr Arg Ile Val Glu Leu 
    770                 775                 780                 


Leu Gly Arg Arg Gly Trp Glu Ala Leu Lys Tyr Trp Trp Asn Leu Leu 
785                 790                 795                 800 


Gln Tyr Trp Ser Gln Glu Leu Lys Asn Ser Ala Val Ser Leu Leu Asn 
                805                 810                 815     


Ala Thr Ala Ile Ala Val Ala Glu Gly Thr Asp Arg Val Ile Glu Val 
            820                 825                 830         


Val Gln Gly Ala Cys Arg Ala Ile Arg His Ile Pro Arg Arg Ile Arg 
        835                 840                 845             


Gln Gly Leu Glu Arg Ile Leu Leu 
    850                 855     


<210>  2
<211>  615
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  HIV Env consensus clade C sequence

<400>  2

Asn Leu Trp Val Thr Val Tyr Tyr Gly Val Pro Val Trp Lys Glu Ala 
1               5                   10                  15      


Lys Thr Thr Leu Phe Cys Ala Ser Asp Ala Lys Ala Tyr Glu Lys Glu 
            20                  25                  30          


Val His Asn Val Trp Ala Thr His Ala Cys Val Pro Thr Asp Pro Asn 
        35                  40                  45              


Pro Gln Glu Met Val Leu Glu Asn Val Thr Glu Asn Phe Asn Met Trp 
    50                  55                  60                  


Lys Asn Asp Met Val Asp Gln Met His Glu Asp Ile Ile Ser Leu Trp 
65                  70                  75                  80  


Asp Gln Ser Leu Lys Pro Cys Val Lys Leu Thr Pro Leu Cys Val Thr 
                85                  90                  95      


Leu Asn Cys Thr Asn Val Asn Val Thr Asn Thr Asn Asn Asn Asn Met 
            100                 105                 110         


Lys Glu Glu Met Lys Asn Cys Ser Phe Asn Thr Thr Thr Glu Ile Arg 
        115                 120                 125             


Asp Lys Lys Gln Lys Glu Tyr Ala Leu Phe Tyr Arg Leu Asp Ile Val 
    130                 135                 140                 


Pro Leu Asn Glu Asn Ser Ser Glu Tyr Arg Leu Ile Asn Cys Asn Thr 
145                 150                 155                 160 


Ser Thr Ile Thr Gln Ala Cys Pro Lys Val Ser Phe Asp Pro Ile Pro 
                165                 170                 175     


Ile His Tyr Cys Ala Pro Ala Gly Tyr Ala Ile Leu Lys Cys Asn Asn 
            180                 185                 190         


Lys Thr Phe Asn Gly Thr Gly Pro Cys Asn Asn Val Ser Thr Val Gln 
        195                 200                 205             


Cys Thr His Gly Ile Lys Pro Val Val Ser Thr Gln Leu Leu Leu Asn 
    210                 215                 220                 


Gly Ser Leu Ala Glu Glu Glu Ile Ile Ile Arg Ser Glu Asn Leu Thr 
225                 230                 235                 240 


Asp Asn Ala Lys Thr Ile Ile Val His Leu Asn Glu Ser Val Glu Ile 
                245                 250                 255     


Asn Cys Thr Arg Pro Asn Asn Asn Thr Arg Lys Ser Ile Arg Ile Gly 
            260                 265                 270         


Pro Gly Gln Thr Phe Tyr Ala Thr Gly Asp Ile Ile Gly Asp Ile Arg 
        275                 280                 285             


Gln Ala His Cys Asn Ile Ser Glu Ala Lys Trp Asn Lys Thr Leu Gln 
    290                 295                 300                 


Arg Val Lys Lys Lys Leu Lys Glu His Phe Pro Asn Lys Thr Ile Lys 
305                 310                 315                 320 


Phe Ala Pro Ser Ser Gly Gly Asp Leu Glu Ile Thr Thr His Ser Phe 
                325                 330                 335     


Asn Cys Arg Gly Glu Phe Phe Tyr Cys Asn Thr Ser Lys Leu Phe Asn 
            340                 345                 350         


Ser Thr Tyr Asn Asn Thr Thr Ser Asn Ser Thr Ile Thr Leu Pro Cys 
        355                 360                 365             


Arg Ile Lys Gln Ile Ile Asn Met Trp Gln Glu Val Gly Arg Ala Met 
    370                 375                 380                 


Tyr Ala Pro Pro Ile Ala Gly Asn Ile Thr Cys Lys Ser Asn Ile Thr 
385                 390                 395                 400 


Gly Leu Leu Leu Thr Arg Asp Gly Gly Asn Asn Asn Asn Asn Thr Glu 
                405                 410                 415     


Thr Phe Arg Pro Gly Gly Gly Asp Met Arg Asp Asn Trp Arg Ser Glu 
            420                 425                 430         


Leu Tyr Lys Tyr Lys Val Val Glu Ile Lys Pro Leu Gly Ile Ala Pro 
        435                 440                 445             


Thr Lys Ala Lys Arg Arg Val Val Glu Arg Glu Lys Arg Arg Ala Val 
    450                 455                 460                 


Gly Ile Gly Ala Val Phe Leu Gly Phe Leu Gly Ala Ala Gly Ser Thr 
465                 470                 475                 480 


Met Gly Ala Ala Ser Ile Thr Leu Thr Val Gln Ala Arg Gln Leu Leu 
                485                 490                 495     


Ser Gly Ile Val Gln Gln Gln Ser Asn Leu Leu Arg Ala Ile Glu Ala 
            500                 505                 510         


Gln Gln His Met Leu Gln Leu Thr Val Trp Gly Ile Lys Gln Leu Gln 
        515                 520                 525             


Ala Arg Val Leu Ala Ile Glu Arg Tyr Leu Lys Asp Gln Gln Leu Leu 
    530                 535                 540                 


Gly Ile Trp Gly Cys Ser Gly Lys Leu Ile Cys Thr Thr Ala Val Pro 
545                 550                 555                 560 


Trp Asn Ser Ser Trp Ser Asn Lys Ser Gln Glu Asp Ile Trp Asp Asn 
                565                 570                 575     


Met Thr Trp Met Gln Trp Asp Arg Glu Ile Ser Asn Tyr Thr Asp Thr 
            580                 585                 590         


Ile Tyr Arg Leu Leu Glu Glu Ser Gln Asn Gln Gln Glu Lys Asn Glu 
        595                 600                 605             


Lys Asp Leu Leu Ala Leu Asp 
    610                 615 


<210>  3
<211>  616
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ConC_SOSIP sequence

<400>  3

Asn Leu Trp Val Thr Val Tyr Tyr Gly Val Pro Val Trp Lys Glu Ala 
1               5                   10                  15      


Lys Thr Thr Leu Phe Cys Ala Ser Asp Ala Lys Ala Tyr Glu Lys Glu 
            20                  25                  30          


Val His Asn Val Trp Ala Thr His Ala Cys Val Pro Thr Asp Pro Asn 
        35                  40                  45              


Pro Gln Glu Met Val Leu Glu Asn Val Thr Glu Asn Phe Asn Met Trp 
    50                  55                  60                  


Lys Asn Asp Met Val Asp Gln Met His Glu Asp Ile Ile Ser Leu Trp 
65                  70                  75                  80  


Asp Gln Ser Leu Lys Pro Cys Val Lys Leu Thr Pro Leu Cys Val Thr 
                85                  90                  95      


Leu Asn Cys Thr Asn Val Asn Val Thr Asn Thr Asn Asn Asn Asn Met 
            100                 105                 110         


Lys Glu Glu Met Lys Asn Cys Ser Phe Asn Thr Thr Thr Glu Ile Arg 
        115                 120                 125             


Asp Lys Lys Gln Lys Glu Tyr Ala Leu Phe Tyr Arg Leu Asp Ile Val 
    130                 135                 140                 


Pro Leu Asn Glu Asn Ser Ser Glu Tyr Arg Leu Ile Asn Cys Asn Thr 
145                 150                 155                 160 


Ser Thr Ile Thr Gln Ala Cys Pro Lys Val Ser Phe Asp Pro Ile Pro 
                165                 170                 175     


Ile His Tyr Cys Ala Pro Ala Gly Tyr Ala Ile Leu Lys Cys Asn Asn 
            180                 185                 190         


Lys Thr Phe Asn Gly Thr Gly Pro Cys Asn Asn Val Ser Thr Val Gln 
        195                 200                 205             


Cys Thr His Gly Ile Lys Pro Val Val Ser Thr Gln Leu Leu Leu Asn 
    210                 215                 220                 


Gly Ser Leu Ala Glu Glu Glu Ile Ile Ile Arg Ser Glu Asn Leu Thr 
225                 230                 235                 240 


Asp Asn Ala Lys Thr Ile Ile Val His Leu Asn Glu Ser Val Glu Ile 
                245                 250                 255     


Asn Cys Thr Arg Pro Asn Asn Asn Thr Arg Lys Ser Ile Arg Ile Gly 
            260                 265                 270         


Pro Gly Gln Thr Phe Tyr Ala Thr Gly Asp Ile Ile Gly Asp Ile Arg 
        275                 280                 285             


Gln Ala His Cys Asn Ile Ser Glu Ala Lys Trp Asn Lys Thr Leu Gln 
    290                 295                 300                 


Arg Val Lys Lys Lys Leu Lys Glu His Phe Pro Asn Lys Thr Ile Lys 
305                 310                 315                 320 


Phe Ala Pro Ser Ser Gly Gly Asp Leu Glu Ile Thr Thr His Ser Phe 
                325                 330                 335     


Asn Cys Arg Gly Glu Phe Phe Tyr Cys Asn Thr Ser Lys Leu Phe Asn 
            340                 345                 350         


Ser Thr Tyr Asn Asn Thr Thr Ser Asn Ser Thr Ile Thr Leu Pro Cys 
        355                 360                 365             


Arg Ile Lys Gln Ile Ile Asn Met Trp Gln Glu Val Gly Arg Ala Met 
    370                 375                 380                 


Tyr Ala Pro Pro Ile Ala Gly Asn Ile Thr Cys Lys Ser Asn Ile Thr 
385                 390                 395                 400 


Gly Leu Leu Leu Thr Arg Asp Gly Gly Asn Asn Asn Asn Asn Thr Glu 
                405                 410                 415     


Thr Phe Arg Pro Gly Gly Gly Asp Met Arg Asp Asn Trp Arg Ser Glu 
            420                 425                 430         


Leu Tyr Lys Tyr Lys Val Val Glu Ile Lys Pro Leu Gly Ile Ala Pro 
        435                 440                 445             


Thr Lys Cys Lys Arg Arg Val Val Glu Arg Arg Arg Arg Arg Arg Ala 
    450                 455                 460                 


Val Gly Ile Gly Ala Val Phe Leu Gly Phe Leu Gly Ala Ala Gly Ser 
465                 470                 475                 480 


Thr Met Gly Ala Ala Ser Ile Thr Leu Thr Val Gln Ala Arg Gln Leu 
                485                 490                 495     


Leu Ser Gly Ile Val Gln Gln Gln Ser Asn Leu Leu Arg Ala Pro Glu 
            500                 505                 510         


Ala Gln Gln His Met Leu Gln Leu Thr Val Trp Gly Ile Lys Gln Leu 
        515                 520                 525             


Gln Ala Arg Val Leu Ala Ile Glu Arg Tyr Leu Lys Asp Gln Gln Leu 
    530                 535                 540                 


Leu Gly Ile Trp Gly Cys Ser Gly Lys Leu Ile Cys Cys Thr Ala Val 
545                 550                 555                 560 


Pro Trp Asn Ser Ser Trp Ser Asn Lys Ser Gln Glu Asp Ile Trp Asp 
                565                 570                 575     


Asn Met Thr Trp Met Gln Trp Asp Arg Glu Ile Ser Asn Tyr Thr Asp 
            580                 585                 590         


Thr Ile Tyr Arg Leu Leu Glu Glu Ser Gln Asn Gln Gln Glu Lys Asn 
        595                 600                 605             


Glu Lys Asp Leu Leu Ala Leu Asp 
    610                 615     


<210>  4
<211>  630
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  HIV Env consensus Clade B sequence

<400>  4

Ala Glu Lys Leu Trp Val Thr Val Tyr Tyr Gly Val Pro Val Trp Lys 
1               5                   10                  15      


Glu Ala Thr Thr Thr Leu Phe Cys Ala Ser Asp Ala Lys Ala Tyr Asp 
            20                  25                  30          


Thr Glu Val His Asn Val Trp Ala Thr His Ala Cys Val Pro Thr Asp 
        35                  40                  45              


Pro Asn Pro Gln Glu Val Val Leu Glu Asn Val Thr Glu Asn Phe Asn 
    50                  55                  60                  


Met Trp Lys Asn Asn Met Val Glu Gln Met His Glu Asp Ile Ile Ser 
65                  70                  75                  80  


Leu Trp Asp Gln Ser Leu Lys Pro Cys Val Lys Leu Thr Pro Leu Cys 
                85                  90                  95      


Val Thr Leu Asn Cys Thr Asp Leu Asn Asn Asn Thr Thr Asn Asn Asn 
            100                 105                 110         


Ser Ser Ser Glu Lys Met Glu Lys Gly Glu Ile Lys Asn Cys Ser Phe 
        115                 120                 125             


Asn Ile Thr Thr Ser Ile Arg Asp Lys Val Gln Lys Glu Tyr Ala Leu 
    130                 135                 140                 


Phe Tyr Lys Leu Asp Val Val Pro Ile Asp Asn Asn Asn Thr Ser Tyr 
145                 150                 155                 160 


Arg Leu Ile Ser Cys Asn Thr Ser Val Ile Thr Gln Ala Cys Pro Lys 
                165                 170                 175     


Val Ser Phe Glu Pro Ile Pro Ile His Tyr Cys Ala Pro Ala Gly Phe 
            180                 185                 190         


Ala Ile Leu Lys Cys Asn Asp Lys Lys Phe Asn Gly Thr Gly Pro Cys 
        195                 200                 205             


Thr Asn Val Ser Thr Val Gln Cys Thr His Gly Ile Arg Pro Val Val 
    210                 215                 220                 


Ser Thr Gln Leu Leu Leu Asn Gly Ser Leu Ala Glu Glu Glu Val Val 
225                 230                 235                 240 


Ile Arg Ser Glu Asn Phe Thr Asp Asn Ala Lys Thr Ile Ile Val Gln 
                245                 250                 255     


Leu Asn Glu Ser Val Glu Ile Asn Cys Thr Arg Pro Asn Asn Asn Thr 
            260                 265                 270         


Arg Lys Ser Ile His Ile Gly Pro Gly Arg Ala Phe Tyr Ala Thr Gly 
        275                 280                 285             


Asp Ile Ile Gly Asp Ile Arg Gln Ala His Cys Asn Ile Ser Arg Thr 
    290                 295                 300                 


Lys Trp Asn Asn Thr Leu Lys Gln Ile Val Lys Lys Leu Arg Glu Gln 
305                 310                 315                 320 


Phe Gly Asn Lys Thr Ile Val Phe Asn Gln Ser Ser Gly Gly Asp Pro 
                325                 330                 335     


Glu Ile Val Met His Ser Phe Asn Cys Gly Gly Glu Phe Phe Tyr Cys 
            340                 345                 350         


Asn Thr Thr Gln Leu Phe Asn Ser Thr Trp Asn Ser Asn Gly Thr Trp 
        355                 360                 365             


Asn Asn Thr Thr Gly Asn Asp Thr Ile Thr Leu Pro Cys Arg Ile Lys 
    370                 375                 380                 


Gln Ile Ile Asn Met Trp Gln Glu Val Gly Lys Ala Met Tyr Ala Pro 
385                 390                 395                 400 


Pro Ile Arg Gly Gln Ile Arg Cys Ser Ser Asn Ile Thr Gly Leu Leu 
                405                 410                 415     


Leu Thr Arg Asp Gly Gly Asn Asn Asn Asn Asn Thr Thr Glu Thr Phe 
            420                 425                 430         


Arg Pro Gly Gly Gly Asp Met Arg Asp Asn Trp Arg Ser Glu Leu Tyr 
        435                 440                 445             


Lys Tyr Lys Val Val Lys Ile Glu Pro Leu Gly Val Ala Pro Thr Lys 
    450                 455                 460                 


Cys Lys Arg Arg Val Val Gln Arg Arg Arg Arg Arg Arg Ala Val Gly 
465                 470                 475                 480 


Ile Gly Ala Met Phe Leu Gly Phe Leu Gly Ala Ala Gly Ser Thr Met 
                485                 490                 495     


Gly Ala Ala Ser Ile Thr Leu Thr Val Gln Ala Arg Gln Leu Leu Ser 
            500                 505                 510         


Gly Ile Val Gln Gln Gln Asn Asn Leu Leu Arg Ala Pro Glu Ala Gln 
        515                 520                 525             


Gln His Leu Leu Gln Leu Thr Val Trp Gly Ile Lys Gln Leu Gln Ala 
    530                 535                 540                 


Arg Val Leu Ala Val Glu Arg Tyr Leu Lys Asp Gln Gln Leu Leu Gly 
545                 550                 555                 560 


Ile Trp Gly Cys Ser Gly Lys Leu Ile Cys Cys Thr Ala Val Pro Trp 
                565                 570                 575     


Asn Thr Ser Trp Ser Asn Lys Ser Leu Asp Glu Ile Trp Asp Asn Met 
            580                 585                 590         


Thr Trp Met Gln Trp Glu Arg Glu Ile Asp Asn Tyr Thr Gly Leu Ile 
        595                 600                 605             


Tyr Thr Leu Ile Glu Glu Ser Gln Asn Gln Gln Glu Lys Asn Glu Gln 
    610                 615                 620                 


Glu Leu Leu Glu Leu Asp 
625                 630 


<210>  5
<211>  630
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ConB_SOSIP sequence

<400>  5

Ala Glu Lys Leu Trp Val Thr Val Tyr Tyr Gly Val Pro Val Trp Lys 
1               5                   10                  15      


Glu Ala Thr Thr Thr Leu Phe Cys Ala Ser Asp Ala Lys Ala Tyr Asp 
            20                  25                  30          


Thr Glu Val His Asn Val Trp Ala Thr His Ala Cys Val Pro Thr Asp 
        35                  40                  45              


Pro Asn Pro Gln Glu Val Val Leu Glu Asn Val Thr Glu Asn Phe Asn 
    50                  55                  60                  


Met Trp Lys Asn Asn Met Val Glu Gln Met His Glu Asp Ile Ile Ser 
65                  70                  75                  80  


Leu Trp Asp Gln Ser Leu Lys Pro Cys Val Lys Leu Thr Pro Leu Cys 
                85                  90                  95      


Val Thr Leu Asn Cys Thr Asp Leu Asn Asn Asn Thr Thr Asn Asn Asn 
            100                 105                 110         


Ser Ser Ser Glu Lys Met Glu Lys Gly Glu Ile Lys Asn Cys Ser Phe 
        115                 120                 125             


Asn Ile Thr Thr Ser Ile Arg Asp Lys Val Gln Lys Glu Tyr Ala Leu 
    130                 135                 140                 


Phe Tyr Lys Leu Asp Val Val Pro Ile Asp Asn Asn Asn Thr Ser Tyr 
145                 150                 155                 160 


Arg Leu Ile Ser Cys Asn Thr Ser Val Ile Thr Gln Ala Cys Pro Lys 
                165                 170                 175     


Val Ser Phe Glu Pro Ile Pro Ile His Tyr Cys Ala Pro Ala Gly Phe 
            180                 185                 190         


Ala Ile Leu Lys Cys Asn Asp Lys Lys Phe Asn Gly Thr Gly Pro Cys 
        195                 200                 205             


Thr Asn Val Ser Thr Val Gln Cys Thr His Gly Ile Arg Pro Val Val 
    210                 215                 220                 


Ser Thr Gln Leu Leu Leu Asn Gly Ser Leu Ala Glu Glu Glu Val Val 
225                 230                 235                 240 


Ile Arg Ser Glu Asn Phe Thr Asp Asn Ala Lys Thr Ile Ile Val Gln 
                245                 250                 255     


Leu Asn Glu Ser Val Glu Ile Asn Cys Thr Arg Pro Asn Asn Asn Thr 
            260                 265                 270         


Arg Lys Ser Ile His Ile Gly Pro Gly Arg Ala Phe Tyr Ala Thr Gly 
        275                 280                 285             


Asp Ile Ile Gly Asp Ile Arg Gln Ala His Cys Asn Ile Ser Arg Thr 
    290                 295                 300                 


Lys Trp Asn Asn Thr Leu Lys Gln Ile Val Lys Lys Leu Arg Glu Gln 
305                 310                 315                 320 


Phe Gly Asn Lys Thr Ile Val Phe Asn Gln Ser Ser Gly Gly Asp Pro 
                325                 330                 335     


Glu Ile Val Met His Ser Phe Asn Cys Gly Gly Glu Phe Phe Tyr Cys 
            340                 345                 350         


Asn Thr Thr Gln Leu Phe Asn Ser Thr Trp Asn Ser Asn Gly Thr Trp 
        355                 360                 365             


Asn Asn Thr Thr Gly Asn Asp Thr Ile Thr Leu Pro Cys Arg Ile Lys 
    370                 375                 380                 


Gln Ile Ile Asn Met Trp Gln Glu Val Gly Lys Ala Met Tyr Ala Pro 
385                 390                 395                 400 


Pro Ile Arg Gly Gln Ile Arg Cys Ser Ser Asn Ile Thr Gly Leu Leu 
                405                 410                 415     


Leu Thr Arg Asp Gly Gly Asn Asn Asn Asn Asn Thr Thr Glu Thr Phe 
            420                 425                 430         


Arg Pro Gly Gly Gly Asp Met Arg Asp Asn Trp Arg Ser Glu Leu Tyr 
        435                 440                 445             


Lys Tyr Lys Val Val Lys Ile Glu Pro Leu Gly Val Ala Pro Thr Lys 
    450                 455                 460                 


Cys Lys Arg Arg Val Val Gln Arg Arg Arg Arg Arg Arg Ala Val Gly 
465                 470                 475                 480 


Ile Gly Ala Met Phe Leu Gly Phe Leu Gly Ala Ala Gly Ser Thr Met 
                485                 490                 495     


Gly Ala Ala Ser Ile Thr Leu Thr Val Gln Ala Arg Gln Leu Leu Ser 
            500                 505                 510         


Gly Ile Val Gln Gln Gln Asn Asn Leu Leu Arg Ala Pro Glu Ala Gln 
        515                 520                 525             


Gln His Leu Leu Gln Leu Thr Val Trp Gly Ile Lys Gln Leu Gln Ala 
    530                 535                 540                 


Arg Val Leu Ala Val Glu Arg Tyr Leu Lys Asp Gln Gln Leu Leu Gly 
545                 550                 555                 560 


Ile Trp Gly Cys Ser Gly Lys Leu Ile Cys Cys Thr Ala Val Pro Trp 
                565                 570                 575     


Asn Thr Ser Trp Ser Asn Lys Ser Leu Asp Glu Ile Trp Asp Asn Met 
            580                 585                 590         


Thr Trp Met Gln Trp Glu Arg Glu Ile Asp Asn Tyr Thr Gly Leu Ile 
        595                 600                 605             


Tyr Thr Leu Ile Glu Glu Ser Gln Asn Gln Gln Glu Lys Asn Glu Gln 
    610                 615                 620                 


Glu Leu Leu Glu Leu Asp 
625                 630 


<210>  6
<211>  625
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  synthetic HIV envelope protein Mos2S Env C4 fragment

<400>  6

Met Gly Asn Leu Trp Val Thr Val Tyr Tyr Gly Val Pro Val Trp Lys 
1               5                   10                  15      


Asp Ala Lys Thr Thr Leu Phe Cys Ala Ser Asp Ala Lys Ala Tyr Glu 
            20                  25                  30          


Lys Glu Val His Asn Val Trp Ala Thr His Ala Cys Val Pro Thr Asp 
        35                  40                  45              


Pro Asn Pro Gln Glu Ile Val Leu Gly Asn Val Thr Glu Asn Phe Asn 
    50                  55                  60                  


Met Trp Lys Asn Asp Met Val Asp Gln Met His Glu Asp Ile Ile Ser 
65                  70                  75                  80  


Leu Trp Asp Ala Ser Leu Glu Pro Cys Val Lys Leu Thr Pro Leu Cys 
                85                  90                  95      


Val Thr Leu Asn Cys Arg Asn Val Arg Asn Val Ser Ser Asn Gly Thr 
            100                 105                 110         


Tyr Asn Ile Ile His Asn Glu Thr Tyr Lys Glu Met Lys Asn Cys Ser 
        115                 120                 125             


Phe Asn Ala Thr Thr Val Val Glu Asp Arg Lys Gln Lys Val His Ala 
    130                 135                 140                 


Leu Phe Tyr Arg Leu Asp Ile Val Pro Leu Asp Glu Asn Asn Ser Ser 
145                 150                 155                 160 


Glu Lys Ser Ser Glu Asn Ser Ser Glu Tyr Tyr Arg Leu Ile Asn Cys 
                165                 170                 175     


Asn Thr Ser Ala Ile Thr Gln Ala Cys Pro Lys Val Ser Phe Asp Pro 
            180                 185                 190         


Ile Pro Ile His Tyr Cys Ala Pro Ala Gly Tyr Ala Ile Leu Lys Cys 
        195                 200                 205             


Asn Asn Lys Thr Phe Asn Gly Thr Gly Pro Cys Asn Asn Val Ser Thr 
    210                 215                 220                 


Val Gln Cys Thr His Gly Ile Lys Pro Val Val Ser Thr Gln Leu Leu 
225                 230                 235                 240 


Leu Asn Gly Ser Leu Ala Glu Glu Glu Ile Ile Ile Arg Ser Glu Asn 
                245                 250                 255     


Leu Thr Asn Asn Ala Lys Thr Ile Ile Val His Leu Asn Glu Thr Val 
            260                 265                 270         


Asn Ile Thr Cys Thr Arg Pro Asn Asn Asn Thr Arg Lys Ser Ile Arg 
        275                 280                 285             


Ile Gly Pro Gly Gln Thr Phe Tyr Ala Thr Gly Asp Ile Ile Gly Asp 
    290                 295                 300                 


Ile Arg Gln Ala His Cys Asn Leu Ser Arg Asp Gly Trp Asn Lys Thr 
305                 310                 315                 320 


Leu Gln Gly Val Lys Lys Lys Leu Ala Glu His Phe Pro Asn Lys Thr 
                325                 330                 335     


Ile Lys Phe Ala Pro His Ser Gly Gly Asp Leu Glu Ile Thr Thr His 
            340                 345                 350         


Thr Phe Asn Cys Arg Gly Glu Phe Phe Tyr Cys Asn Thr Ser Asn Leu 
        355                 360                 365             


Phe Asn Glu Ser Asn Ile Glu Arg Asn Asp Ser Ile Ile Thr Leu Pro 
    370                 375                 380                 


Cys Arg Ile Lys Gln Ile Ile Asn Met Trp Gln Glu Val Gly Arg Ala 
385                 390                 395                 400 


Ile Tyr Ala Pro Pro Ile Ala Gly Asn Ile Thr Cys Arg Ser Asn Ile 
                405                 410                 415     


Thr Gly Leu Leu Leu Thr Arg Asp Gly Gly Ser Asn Asn Gly Val Pro 
            420                 425                 430         


Asn Asp Thr Glu Thr Phe Arg Pro Gly Gly Gly Asp Met Arg Asn Asn 
        435                 440                 445             


Trp Arg Ser Glu Leu Tyr Lys Tyr Lys Val Val Glu Val Lys Pro Leu 
    450                 455                 460                 


Gly Val Ala Pro Thr Glu Ala Lys Arg Arg Val Val Glu Arg Glu Lys 
465                 470                 475                 480 


Arg Ala Val Gly Ile Gly Ala Val Phe Leu Gly Ile Leu Gly Ala Ala 
                485                 490                 495     


Gly Ser Thr Met Gly Ala Ala Ser Ile Thr Leu Thr Val Gln Ala Arg 
            500                 505                 510         


Gln Leu Leu Ser Gly Ile Val Gln Gln Gln Ser Asn Leu Leu Arg Ala 
        515                 520                 525             


Ile Glu Ala Gln Gln His Met Leu Gln Leu Thr Val Trp Gly Ile Lys 
    530                 535                 540                 


Gln Leu Gln Thr Arg Val Leu Ala Ile Glu Arg Tyr Leu Gln Asp Gln 
545                 550                 555                 560 


Gln Leu Leu Gly Leu Trp Gly Cys Ser Gly Lys Leu Ile Cys Thr Thr 
                565                 570                 575     


Ala Val Pro Trp Asn Thr Ser Trp Ser Asn Lys Ser Gln Thr Asp Ile 
            580                 585                 590         


Trp Asp Asn Met Thr Trp Met Gln Trp Asp Lys Glu Ile Gly Asn Tyr 
        595                 600                 605             


Thr Gly Glu Ile Tyr Arg Leu Leu Glu Glu Ser Gln Asn Gln Gln Glu 
    610                 615                 620                 


Lys 
625 


<210>  7
<211>  627
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  DS_sC4_SOSIP_E166R sequence

<400>  7

Met Gly Asn Leu Trp Val Thr Val Tyr Tyr Gly Val Pro Val Trp Lys 
1               5                   10                  15      


Asp Ala Lys Thr Thr Leu Phe Cys Ala Ser Asp Ala Lys Ala Tyr Glu 
            20                  25                  30          


Lys Glu Val His Asn Val Trp Ala Thr His Ala Cys Val Pro Thr Asp 
        35                  40                  45              


Pro Asn Pro Gln Glu Ile Val Leu Gly Asn Val Thr Glu Asn Phe Asn 
    50                  55                  60                  


Met Trp Lys Asn Asp Met Val Asp Gln Met His Glu Asp Ile Ile Ser 
65                  70                  75                  80  


Leu Trp Asp Ala Ser Leu Glu Pro Cys Val Lys Leu Thr Pro Leu Cys 
                85                  90                  95      


Val Thr Leu Asn Cys Arg Asn Val Arg Asn Val Ser Ser Asn Gly Thr 
            100                 105                 110         


Tyr Asn Ile Ile His Asn Glu Thr Tyr Lys Glu Met Lys Asn Cys Ser 
        115                 120                 125             


Phe Asn Ala Thr Thr Val Val Arg Asp Arg Lys Gln Lys Val His Ala 
    130                 135                 140                 


Leu Phe Tyr Arg Leu Asp Ile Val Pro Leu Asp Glu Asn Asn Ser Ser 
145                 150                 155                 160 


Glu Lys Ser Ser Glu Asn Ser Ser Glu Tyr Tyr Arg Leu Ile Asn Cys 
                165                 170                 175     


Asn Thr Ser Ala Cys Thr Gln Ala Cys Pro Lys Val Ser Phe Asp Pro 
            180                 185                 190         


Ile Pro Ile His Tyr Cys Ala Pro Ala Gly Tyr Ala Ile Leu Lys Cys 
        195                 200                 205             


Asn Asn Lys Thr Phe Asn Gly Thr Gly Pro Cys Asn Asn Val Ser Thr 
    210                 215                 220                 


Val Gln Cys Thr His Gly Ile Lys Pro Val Val Ser Thr Gln Leu Leu 
225                 230                 235                 240 


Leu Asn Gly Ser Leu Ala Glu Glu Glu Ile Ile Ile Arg Ser Glu Asn 
                245                 250                 255     


Leu Thr Asn Asn Ala Lys Thr Ile Ile Val His Leu Asn Glu Thr Val 
            260                 265                 270         


Asn Ile Asn Cys Thr Arg Pro Asn Asn Asn Thr Arg Lys Ser Ile Arg 
        275                 280                 285             


Ile Gly Pro Gly Gln Thr Phe Tyr Ala Thr Gly Asp Ile Ile Gly Asp 
    290                 295                 300                 


Ile Arg Gln Ala His Cys Asn Leu Ser Arg Asp Gly Trp Asn Lys Thr 
305                 310                 315                 320 


Leu Gln Gly Val Lys Lys Lys Leu Ala Glu His Phe Pro Asn Lys Thr 
                325                 330                 335     


Ile Lys Phe Ala Pro His Ser Gly Gly Asp Leu Glu Ile Thr Thr His 
            340                 345                 350         


Thr Phe Asn Cys Arg Gly Glu Phe Phe Tyr Cys Asn Thr Ser Asn Leu 
        355                 360                 365             


Phe Asn Glu Ser Asn Ile Glu Arg Asn Asp Ser Ile Ile Thr Leu Pro 
    370                 375                 380                 


Cys Arg Ile Lys Gln Ile Ile Asn Met Trp Gln Glu Val Gly Arg Cys 
385                 390                 395                 400 


Ile Tyr Ala Pro Pro Ile Ala Gly Asn Ile Thr Cys Arg Ser Asn Ile 
                405                 410                 415     


Thr Gly Leu Leu Leu Thr Arg Asp Gly Gly Ser Asn Asn Gly Val Pro 
            420                 425                 430         


Asn Asp Thr Glu Thr Phe Arg Pro Gly Gly Gly Asp Met Arg Asn Asn 
        435                 440                 445             


Trp Arg Ser Glu Leu Tyr Lys Tyr Lys Val Val Glu Val Lys Pro Leu 
    450                 455                 460                 


Gly Val Ala Pro Thr Glu Cys Lys Arg Arg Val Val Glu Arg Arg Arg 
465                 470                 475                 480 


Arg Arg Arg Ala Val Gly Ile Gly Ala Val Phe Leu Gly Ile Leu Gly 
                485                 490                 495     


Ala Ala Gly Ser Thr Met Gly Ala Ala Ser Ile Thr Leu Thr Val Gln 
            500                 505                 510         


Ala Arg Gln Leu Leu Ser Gly Ile Val Gln Gln Gln Ser Asn Leu Leu 
        515                 520                 525             


Arg Ala Pro Glu Ala Gln Gln His Met Leu Gln Leu Thr Val Trp Gly 
    530                 535                 540                 


Ile Lys Gln Leu Gln Thr Arg Val Leu Ala Ile Glu Arg Tyr Leu Gln 
545                 550                 555                 560 


Asp Gln Gln Leu Leu Gly Leu Trp Gly Cys Ser Gly Lys Leu Ile Cys 
                565                 570                 575     


Cys Thr Ala Val Pro Trp Asn Thr Ser Trp Ser Asn Lys Ser Gln Thr 
            580                 585                 590         


Asp Ile Trp Asp Asn Met Thr Trp Met Gln Trp Asp Lys Glu Ile Gly 
        595                 600                 605             


Asn Tyr Thr Gly Glu Ile Tyr Arg Leu Leu Glu Glu Ser Gln Asn Gln 
    610                 615                 620                 


Gln Glu Lys 
625         


<210>  8
<211>  631
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Mos1.Env sequence

<400>  8

Ala Gly Lys Leu Trp Val Thr Val Tyr Tyr Gly Val Pro Val Trp Lys 
1               5                   10                  15      


Glu Ala Thr Thr Thr Leu Phe Cys Ala Ser Asp Ala Lys Ala Tyr Asp 
            20                  25                  30          


Thr Glu Val His Asn Val Trp Ala Thr His Ala Cys Val Pro Thr Asp 
        35                  40                  45              


Pro Asn Pro Gln Glu Val Val Leu Glu Asn Val Thr Glu Asn Phe Asn 
    50                  55                  60                  


Met Trp Lys Asn Asn Met Val Glu Gln Met His Glu Asp Ile Ile Ser 
65                  70                  75                  80  


Leu Trp Asp Gln Ser Leu Lys Pro Cys Val Lys Leu Thr Pro Leu Cys 
                85                  90                  95      


Val Thr Leu Asn Cys Thr Asp Asp Val Arg Asn Val Thr Asn Asn Ala 
            100                 105                 110         


Thr Asn Thr Asn Ser Ser Trp Gly Glu Pro Met Glu Lys Gly Glu Ile 
        115                 120                 125             


Lys Asn Cys Ser Phe Asn Ile Thr Thr Ser Ile Arg Asn Lys Val Gln 
    130                 135                 140                 


Lys Gln Tyr Ala Leu Phe Tyr Lys Leu Asp Val Val Pro Ile Asp Asn 
145                 150                 155                 160 


Asp Ser Asn Asn Thr Asn Tyr Arg Leu Ile Ser Cys Asn Thr Ser Val 
                165                 170                 175     


Ile Thr Gln Ala Cys Pro Lys Val Ser Phe Glu Pro Ile Pro Ile His 
            180                 185                 190         


Tyr Cys Ala Pro Ala Gly Phe Ala Ile Leu Lys Cys Asn Asp Lys Lys 
        195                 200                 205             


Phe Asn Gly Thr Gly Pro Cys Thr Asn Val Ser Thr Val Gln Cys Thr 
    210                 215                 220                 


His Gly Ile Arg Pro Val Val Ser Thr Gln Leu Leu Leu Asn Gly Ser 
225                 230                 235                 240 


Leu Ala Glu Glu Glu Val Val Ile Arg Ser Glu Asn Phe Thr Asn Asn 
                245                 250                 255     


Ala Lys Thr Ile Met Val Gln Leu Asn Val Ser Val Glu Ile Asn Cys 
            260                 265                 270         


Thr Arg Pro Asn Asn Asn Thr Arg Lys Ser Ile His Ile Gly Pro Gly 
        275                 280                 285             


Arg Ala Phe Tyr Thr Ala Gly Asp Ile Ile Gly Asp Ile Arg Gln Ala 
    290                 295                 300                 


His Cys Asn Ile Ser Arg Ala Asn Trp Asn Asn Thr Leu Arg Gln Ile 
305                 310                 315                 320 


Val Glu Lys Leu Gly Lys Gln Phe Gly Asn Asn Lys Thr Ile Val Phe 
                325                 330                 335     


Asn His Ser Ser Gly Gly Asp Pro Glu Ile Val Met His Ser Phe Asn 
            340                 345                 350         


Cys Gly Gly Glu Phe Phe Tyr Cys Asn Ser Thr Lys Leu Phe Asn Ser 
        355                 360                 365             


Thr Trp Thr Trp Asn Asn Ser Thr Trp Asn Asn Thr Lys Arg Ser Asn 
    370                 375                 380                 


Asp Thr Glu Glu His Ile Thr Leu Pro Cys Arg Ile Lys Gln Ile Ile 
385                 390                 395                 400 


Asn Met Trp Gln Glu Val Gly Lys Ala Met Tyr Ala Pro Pro Ile Arg 
                405                 410                 415     


Gly Gln Ile Arg Cys Ser Ser Asn Ile Thr Gly Leu Leu Leu Thr Arg 
            420                 425                 430         


Asp Gly Gly Asn Asp Thr Ser Gly Thr Glu Ile Phe Arg Pro Gly Gly 
        435                 440                 445             


Gly Asp Met Arg Asp Asn Trp Arg Ser Glu Leu Tyr Lys Tyr Lys Val 
    450                 455                 460                 


Val Lys Ile Glu Pro Leu Gly Val Ala Pro Thr Lys Ala Lys Arg Arg 
465                 470                 475                 480 


Val Val Gln Ser Glu Lys Ser Ala Val Gly Ile Gly Ala Val Phe Leu 
                485                 490                 495     


Gly Phe Leu Gly Ala Ala Gly Ser Thr Met Gly Ala Ala Ser Met Thr 
            500                 505                 510         


Leu Thr Val Gln Ala Arg Leu Leu Leu Ser Gly Ile Val Gln Gln Gln 
        515                 520                 525             


Asn Asn Leu Leu Arg Ala Ile Glu Ala Gln Gln His Leu Leu Gln Leu 
    530                 535                 540                 


Thr Val Trp Gly Ile Lys Gln Leu Gln Ala Arg Val Leu Ala Val Glu 
545                 550                 555                 560 


Arg Tyr Leu Lys Asp Gln Gln Leu Leu Gly Ile Trp Gly Cys Ser Gly 
                565                 570                 575     


Lys Leu Ile Cys Thr Thr Thr Val Pro Trp Asn Ala Ser Trp Ser Asn 
            580                 585                 590         


Lys Ser Leu Asp Lys Ile Trp Asn Asn Met Thr Trp Met Glu Trp Glu 
        595                 600                 605             


Arg Glu Ile Asn Asn Tyr Thr Ser Leu Ile Tyr Thr Leu Ile Glu Glu 
    610                 615                 620                 


Ser Gln Asn Gln Gln Glu Lys 
625                 630     


<210>  9
<211>  630
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Mos2.Env sequence

<400>  9

Met Gly Asn Leu Trp Val Thr Val Tyr Tyr Gly Val Pro Val Trp Lys 
1               5                   10                  15      


Glu Ala Lys Thr Thr Leu Phe Cys Ala Ser Asp Ala Lys Ala Tyr Glu 
            20                  25                  30          


Lys Glu Val His Asn Val Trp Ala Thr His Ala Cys Val Pro Thr Asp 
        35                  40                  45              


Pro Asn Pro Gln Glu Met Val Leu Glu Asn Val Thr Glu Asn Phe Asn 
    50                  55                  60                  


Met Trp Lys Asn Asp Met Val Asp Gln Met His Glu Asp Ile Ile Arg 
65                  70                  75                  80  


Leu Trp Asp Gln Ser Leu Lys Pro Cys Val Lys Leu Thr Pro Leu Cys 
                85                  90                  95      


Val Thr Leu Glu Cys Arg Asn Val Arg Asn Val Ser Ser Asn Gly Thr 
            100                 105                 110         


Tyr Asn Ile Ile His Asn Glu Thr Tyr Lys Glu Met Lys Asn Cys Ser 
        115                 120                 125             


Phe Asn Ala Thr Thr Val Val Glu Asp Arg Lys Gln Lys Val His Ala 
    130                 135                 140                 


Leu Phe Tyr Arg Leu Asp Ile Val Pro Leu Asp Glu Asn Asn Ser Ser 
145                 150                 155                 160 


Glu Lys Ser Ser Glu Asn Ser Ser Glu Tyr Tyr Arg Leu Ile Asn Cys 
                165                 170                 175     


Asn Thr Ser Ala Ile Thr Gln Ala Cys Pro Lys Val Ser Phe Asp Pro 
            180                 185                 190         


Ile Pro Ile His Tyr Cys Ala Pro Ala Gly Tyr Ala Ile Leu Lys Cys 
        195                 200                 205             


Asn Asn Lys Thr Phe Asn Gly Thr Gly Pro Cys Asn Asn Val Ser Thr 
    210                 215                 220                 


Val Gln Cys Thr His Gly Ile Lys Pro Val Val Ser Thr Gln Leu Leu 
225                 230                 235                 240 


Leu Asn Gly Ser Leu Ala Glu Glu Glu Ile Ile Ile Arg Ser Glu Asn 
                245                 250                 255     


Leu Thr Asn Asn Ala Lys Thr Ile Ile Val His Leu Asn Glu Thr Val 
            260                 265                 270         


Asn Ile Thr Cys Thr Arg Pro Asn Asn Asn Thr Arg Lys Ser Ile Arg 
        275                 280                 285             


Ile Gly Pro Gly Gln Thr Phe Tyr Ala Thr Gly Asp Ile Ile Gly Asp 
    290                 295                 300                 


Ile Arg Gln Ala His Cys Asn Leu Ser Arg Asp Gly Trp Asn Lys Thr 
305                 310                 315                 320 


Leu Gln Gly Val Lys Lys Lys Leu Ala Glu His Phe Pro Asn Lys Thr 
                325                 330                 335     


Ile Asn Phe Thr Ser Ser Ser Gly Gly Asp Leu Glu Ile Thr Thr His 
            340                 345                 350         


Ser Phe Asn Cys Arg Gly Glu Phe Phe Tyr Cys Asn Thr Ser Gly Leu 
        355                 360                 365             


Phe Asn Gly Thr Tyr Met Pro Asn Gly Thr Asn Ser Asn Ser Ser Ser 
    370                 375                 380                 


Asn Ile Thr Leu Pro Cys Arg Ile Lys Gln Ile Ile Asn Met Trp Gln 
385                 390                 395                 400 


Glu Val Gly Arg Ala Met Tyr Ala Pro Pro Ile Ala Gly Asn Ile Thr 
                405                 410                 415     


Cys Arg Ser Asn Ile Thr Gly Leu Leu Leu Thr Arg Asp Gly Gly Ser 
            420                 425                 430         


Asn Asn Gly Val Pro Asn Asp Thr Glu Thr Phe Arg Pro Gly Gly Gly 
        435                 440                 445             


Asp Met Arg Asn Asn Trp Arg Ser Glu Leu Tyr Lys Tyr Lys Val Val 
    450                 455                 460                 


Glu Val Lys Pro Leu Gly Val Ala Pro Thr Glu Ala Lys Arg Arg Val 
465                 470                 475                 480 


Val Glu Arg Glu Lys Arg Ala Val Gly Ile Gly Ala Val Phe Leu Gly 
                485                 490                 495     


Ile Leu Gly Ala Ala Gly Ser Thr Met Gly Ala Ala Ser Ile Thr Leu 
            500                 505                 510         


Thr Val Gln Ala Arg Gln Leu Leu Ser Gly Ile Val Gln Gln Gln Ser 
        515                 520                 525             


Asn Leu Leu Arg Ala Ile Glu Ala Gln Gln His Met Leu Gln Leu Thr 
    530                 535                 540                 


Val Trp Gly Ile Lys Gln Leu Gln Thr Arg Val Leu Ala Ile Glu Arg 
545                 550                 555                 560 


Tyr Leu Gln Asp Gln Gln Leu Leu Gly Leu Trp Gly Cys Ser Gly Lys 
                565                 570                 575     


Leu Ile Cys Thr Thr Ala Val Pro Trp Asn Thr Ser Trp Ser Asn Lys 
            580                 585                 590         


Ser Gln Thr Asp Ile Trp Asp Asn Met Thr Trp Met Gln Trp Asp Lys 
        595                 600                 605             


Glu Ile Gly Asn Tyr Thr Gly Glu Ile Tyr Arg Leu Leu Glu Glu Ser 
    610                 615                 620                 


Gln Asn Gln Gln Glu Lys 
625                 630 


<210>  10
<211>  6
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  furin cleavage site mutant sequence

<400>  10

Arg Arg Arg Arg Arg Arg 
1               5       


<210>  11
<211>  31
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  signal sequence

<400>  11

Met Arg Val Arg Gly Ile Leu Arg Asn Trp Gln Gln Trp Trp Ile Trp 
1               5                   10                  15      


Gly Ile Leu Gly Phe Trp Met Leu Met Ile Cys Asn Val Val Gly 
            20                  25                  30      


<210>  12
<211>  8
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  example of 8 amino acid sequence that can replace HR1 loop

<400>  12

Asn Pro Asp Trp Leu Pro Asp Met 
1               5               


<210>  13
<211>  8
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  example of 8 amino acid sequence that can replace HR1 loop

<400>  13

Gly Ser Gly Ser Gly Ser Gly Ser 
1               5               


<210>  14
<211>  8
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  example of 8 amino acid sequence that can replace HR1 loop

<400>  14

Asp Asp Val His Pro Asp Trp Asp 
1               5               


<210>  15
<211>  8
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  example of 8 amino acid sequence that can replace HR1 loop

<400>  15

Arg Asp Thr Phe Ala Leu Met Met 
1               5               


<210>  16
<211>  8
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  example of 8 amino acid sequence that can replace HR1 loop

<400>  16

Asp Glu Glu Lys Val Met Asp Phe 
1               5               


<210>  17
<211>  8
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  example of 8 amino acid sequence that can replace HR1 loop

<400>  17

Asp Glu Asp Pro His Trp Asp Pro 
1               5               


<210>  18
<211>  29
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  signal sequence

<400>  18

Met Arg Val Lys Gly Ile Arg Lys Asn Tyr Gln His Leu Trp Arg Trp 
1               5                   10                  15      


Gly Thr Met Leu Leu Gly Met Leu Met Ile Cys Ser Ala 
            20                  25                  


<210>  19
<211>  61
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  tag sequence

<400>  19

Ala Ala Ala Leu Pro Glu Thr Gly Gly Gly Ser Asp Tyr Lys Asp Asp 
1               5                   10                  15      


Asp Asp Lys Pro Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly 
            20                  25                  30          


Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly 
        35                  40                  45              


Gly Ser Gly Gly Gly Gly Ser His His His His His His 
    50                  55                  60      


<210>  20
<211>  616
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  stabilized ConC_SOSIP Env protein (HIV160544)

<400>  20

Asn Leu Trp Val Thr Val Tyr Tyr Gly Val Pro Val Trp Lys Glu Ala 
1               5                   10                  15      


Lys Thr Thr Leu Phe Cys Ala Ser Asp Ala Lys Ala Tyr Glu Lys Glu 
            20                  25                  30          


Val His Asn Val Trp Ala Thr His Ala Cys Val Pro Thr Asp Pro Asn 
        35                  40                  45              


Pro Gln Glu Met Val Leu Glu Asn Val Thr Glu Asn Phe Asn Met Trp 
    50                  55                  60                  


Lys Asn Asp Met Val Asp Gln Met His Glu Asp Ile Ile Ser Leu Trp 
65                  70                  75                  80  


Asp Gln Ser Leu Lys Pro Cys Val Lys Leu Thr Pro Leu Cys Val Thr 
                85                  90                  95      


Leu Asn Cys Thr Asn Val Asn Val Thr Asn Thr Asn Asn Asn Asn Met 
            100                 105                 110         


Lys Glu Glu Met Lys Asn Cys Ser Phe Asn Thr Thr Thr Glu Ile Arg 
        115                 120                 125             


Asp Lys Lys Gln Lys Glu Tyr Ala Leu Phe Tyr Arg Leu Asp Ile Val 
    130                 135                 140                 


Pro Leu Asn Glu Asn Ser Ser Glu Tyr Arg Leu Ile Asn Cys Asn Thr 
145                 150                 155                 160 


Ser Thr Ile Thr Gln Ile Cys Pro Lys Val Ser Phe Asp Pro Ile Pro 
                165                 170                 175     


Ile His Tyr Cys Ala Pro Ala Gly Tyr Ala Ile Leu Lys Cys Asn Asn 
            180                 185                 190         


Lys Thr Phe Asn Gly Thr Gly Pro Cys Asn Asn Val Ser Thr Val Gln 
        195                 200                 205             


Cys Thr His Gly Ile Lys Pro Val Val Ser Thr Gln Leu Leu Leu Asn 
    210                 215                 220                 


Gly Ser Leu Ala Glu Glu Glu Ile Ile Ile Arg Ser Glu Asn Leu Thr 
225                 230                 235                 240 


Asp Asn Ala Lys Thr Ile Ile Val His Leu Asn Glu Ser Val Glu Ile 
                245                 250                 255     


Asn Cys Thr Arg Pro Asn Asn Asn Thr Arg Lys Ser Ile Arg Ile Gly 
            260                 265                 270         


Pro Gly Gln Thr Phe Tyr Ala Thr Gly Asp Ile Ile Gly Asp Ile Arg 
        275                 280                 285             


Gln Ala His Cys Asn Ile Ser Glu Ala Lys Trp Asn Lys Thr Leu Gln 
    290                 295                 300                 


Arg Val Lys Lys Lys Leu Lys Glu His Phe Pro Asn Lys Thr Ile Lys 
305                 310                 315                 320 


Phe Ala Pro Ser Ser Gly Gly Asp Leu Glu Ile Thr Thr His Ser Phe 
                325                 330                 335     


Asn Cys Arg Gly Glu Phe Phe Tyr Cys Asn Thr Ser Lys Leu Phe Asn 
            340                 345                 350         


Ser Thr Tyr Asn Asn Thr Thr Ser Asn Ser Thr Ile Thr Leu Pro Cys 
        355                 360                 365             


Arg Ile Lys Gln Ile Ile Asn Met Trp Gln Glu Val Gly Arg Ala Met 
    370                 375                 380                 


Tyr Ala Pro Pro Ile Ala Gly Asn Ile Thr Cys Lys Ser Asn Ile Thr 
385                 390                 395                 400 


Gly Leu Leu Leu Thr Arg Asp Gly Gly Asn Asn Asn Asn Asn Thr Glu 
                405                 410                 415     


Thr Phe Arg Pro Gly Gly Gly Asp Met Arg Asp Asn Trp Arg Ser Glu 
            420                 425                 430         


Leu Tyr Lys Tyr Lys Val Val Glu Ile Lys Pro Leu Gly Ile Ala Pro 
        435                 440                 445             


Thr Lys Cys Lys Arg Arg Val Val Glu Arg Arg Arg Arg Arg Arg Ala 
    450                 455                 460                 


Val Gly Ile Gly Ala Val Phe Leu Gly Phe Leu Gly Ala Ala Gly Ser 
465                 470                 475                 480 


Thr Met Gly Ala Ala Ser Asn Thr Leu Thr Val Gln Ala Arg Gln Leu 
                485                 490                 495     


Leu Ser Gly Ile Val Gln Gln Gln Ser Asn Leu Leu Arg Ala Pro Glu 
            500                 505                 510         


Ala Gln Gln His Met Leu Gln Leu Thr Val Trp Gly Phe Lys Gln Leu 
        515                 520                 525             


Gln Ala Arg Val Leu Ala Ile Glu Arg Tyr Leu Glu Val Gln Gln Leu 
    530                 535                 540                 


Leu Gly Ile Trp Gly Cys Ser Gly Lys Leu Ile Cys Cys Thr Ala Val 
545                 550                 555                 560 


Pro Trp Asn Ser Ser Trp Ser Asn Lys Ser Gln Glu Asp Ile Trp Asp 
                565                 570                 575     


Asn Met Thr Trp Met Gln Trp Asp Arg Glu Ile Ser Asn Tyr Thr Asp 
            580                 585                 590         


Thr Ile Tyr Arg Leu Leu Glu Glu Ser Gln Phe Gln Gln Glu Ile Asn 
        595                 600                 605             


Glu Lys Asp Leu Leu Ala Leu Asp 
    610                 615     


<210>  21
<211>  634
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  BG505_SOSIP Env protein (HIV150673)

<400>  21

Ala Glu Asn Leu Trp Val Thr Val Tyr Tyr Gly Val Pro Val Trp Lys 
1               5                   10                  15      


Asp Ala Glu Thr Thr Leu Phe Cys Ala Ser Asp Ala Lys Ala Tyr Glu 
            20                  25                  30          


Thr Glu Lys His Asn Val Trp Ala Thr His Ala Cys Val Pro Thr Asp 
        35                  40                  45              


Pro Asn Pro Gln Glu Ile His Leu Glu Asn Val Thr Glu Glu Phe Asn 
    50                  55                  60                  


Met Trp Lys Asn Asn Met Val Glu Gln Met His Thr Asp Ile Ile Ser 
65                  70                  75                  80  


Leu Trp Asp Gln Ser Leu Lys Pro Cys Val Lys Leu Thr Pro Leu Cys 
                85                  90                  95      


Val Thr Leu Gln Cys Thr Asn Val Thr Asn Asn Ile Thr Asp Asp Met 
            100                 105                 110         


Arg Gly Glu Leu Lys Asn Cys Ser Phe Asn Met Thr Thr Glu Leu Arg 
        115                 120                 125             


Asp Lys Lys Gln Lys Val Tyr Ser Leu Phe Tyr Arg Leu Asp Val Val 
    130                 135                 140                 


Gln Ile Asn Glu Asn Gln Gly Asn Arg Ser Asn Asn Ser Asn Lys Glu 
145                 150                 155                 160 


Tyr Arg Leu Ile Asn Cys Asn Thr Ser Ala Ile Thr Gln Ala Cys Pro 
                165                 170                 175     


Lys Val Ser Phe Glu Pro Ile Pro Ile His Tyr Cys Ala Pro Ala Gly 
            180                 185                 190         


Phe Ala Ile Leu Lys Cys Lys Asp Lys Lys Phe Asn Gly Thr Gly Pro 
        195                 200                 205             


Cys Pro Ser Val Ser Thr Val Gln Cys Thr His Gly Ile Lys Pro Val 
    210                 215                 220                 


Val Ser Thr Gln Leu Leu Leu Asn Gly Ser Leu Ala Glu Glu Glu Val 
225                 230                 235                 240 


Met Ile Arg Ser Glu Asn Ile Thr Asn Asn Ala Lys Asn Ile Leu Val 
                245                 250                 255     


Gln Phe Asn Thr Pro Val Gln Ile Asn Cys Thr Arg Pro Asn Asn Asn 
            260                 265                 270         


Thr Arg Lys Ser Ile Arg Ile Gly Pro Gly Gln Ala Phe Tyr Ala Thr 
        275                 280                 285             


Gly Asp Ile Ile Gly Asp Ile Arg Gln Ala His Cys Asn Val Ser Lys 
    290                 295                 300                 


Ala Thr Trp Asn Glu Thr Leu Gly Lys Val Val Lys Gln Leu Arg Lys 
305                 310                 315                 320 


His Phe Gly Asn Asn Thr Ile Ile Arg Phe Ala Asn Ser Ser Gly Gly 
                325                 330                 335     


Asp Leu Glu Val Thr Thr His Ser Phe Asn Cys Gly Gly Glu Phe Phe 
            340                 345                 350         


Tyr Cys Asn Thr Ser Gly Leu Phe Asn Ser Thr Trp Ile Ser Asn Thr 
        355                 360                 365             


Ser Val Gln Gly Ser Asn Ser Thr Gly Ser Asn Asp Ser Ile Thr Leu 
    370                 375                 380                 


Pro Cys Arg Ile Lys Gln Ile Ile Asn Met Trp Gln Arg Ile Gly Gln 
385                 390                 395                 400 


Ala Met Tyr Ala Pro Pro Ile Gln Gly Val Ile Arg Cys Val Ser Asn 
                405                 410                 415     


Ile Thr Gly Leu Ile Leu Thr Arg Asp Gly Gly Ser Thr Asn Ser Thr 
            420                 425                 430         


Thr Glu Thr Phe Arg Pro Gly Gly Gly Asp Met Arg Asp Asn Trp Arg 
        435                 440                 445             


Ser Glu Leu Tyr Lys Tyr Lys Val Val Lys Ile Glu Pro Leu Gly Val 
    450                 455                 460                 


Ala Pro Thr Arg Cys Lys Arg Arg Val Val Gly Arg Arg Arg Arg Arg 
465                 470                 475                 480 


Arg Ala Val Gly Ile Gly Ala Val Phe Leu Gly Phe Leu Gly Ala Ala 
                485                 490                 495     


Gly Ser Thr Met Gly Ala Ala Ser Met Thr Leu Thr Val Gln Ala Arg 
            500                 505                 510         


Asn Leu Leu Ser Gly Ile Val Gln Gln Gln Ser Asn Leu Leu Arg Ala 
        515                 520                 525             


Pro Glu Ala Gln Gln His Leu Leu Lys Leu Thr Val Trp Gly Ile Lys 
    530                 535                 540                 


Gln Leu Gln Ala Arg Val Leu Ala Val Glu Arg Tyr Leu Arg Asp Gln 
545                 550                 555                 560 


Gln Leu Leu Gly Ile Trp Gly Cys Ser Gly Lys Leu Ile Cys Cys Thr 
                565                 570                 575     


Asn Val Pro Trp Asn Ser Ser Trp Ser Asn Arg Asn Leu Ser Glu Ile 
            580                 585                 590         


Trp Asp Asn Met Thr Trp Leu Gln Trp Asp Lys Glu Ile Ser Asn Tyr 
        595                 600                 605             


Thr Gln Ile Ile Tyr Gly Leu Leu Glu Glu Ser Gln Asn Gln Gln Glu 
    610                 615                 620                 


Lys Asn Glu Gln Asp Leu Leu Ala Leu Asp 
625                 630                 


<210>  22
<211>  634
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  stabilized BG505_SOSIP Env protein (HIV170863)

<400>  22

Ala Glu Asn Leu Trp Val Thr Val Tyr Tyr Gly Val Pro Val Trp Lys 
1               5                   10                  15      


Asp Ala Glu Thr Thr Leu Phe Cys Ala Ser Asp Ala Lys Ala Tyr Glu 
            20                  25                  30          


Thr Glu Lys His Asn Val Trp Ala Thr His Ala Cys Val Pro Thr Asp 
        35                  40                  45              


Pro Asn Pro Gln Glu Ile His Leu Glu Asn Val Thr Glu Glu Phe Asn 
    50                  55                  60                  


Met Trp Lys Asn Asn Met Val Glu Gln Met His Thr Asp Ile Ile Ser 
65                  70                  75                  80  


Leu Trp Asp Gln Ser Leu Lys Pro Cys Val Lys Leu Thr Pro Leu Cys 
                85                  90                  95      


Val Thr Leu Gln Cys Thr Asn Val Thr Asn Asn Ile Thr Asp Asp Met 
            100                 105                 110         


Arg Gly Glu Leu Lys Asn Cys Ser Phe Asn Met Thr Thr Glu Leu Arg 
        115                 120                 125             


Asp Lys Lys Gln Lys Val Tyr Ser Leu Phe Tyr Arg Leu Asp Val Val 
    130                 135                 140                 


Gln Ile Asn Glu Asn Gln Gly Asn Arg Ser Asn Asn Ser Asn Lys Glu 
145                 150                 155                 160 


Tyr Arg Leu Ile Asn Cys Asn Thr Ser Ala Ile Thr Gln Ala Cys Pro 
                165                 170                 175     


Lys Val Ser Phe Glu Pro Ile Pro Ile His Tyr Cys Ala Pro Ala Gly 
            180                 185                 190         


Phe Ala Ile Leu Lys Cys Lys Asp Lys Lys Phe Asn Gly Thr Gly Pro 
        195                 200                 205             


Cys Pro Ser Val Ser Thr Val Gln Cys Thr His Gly Ile Lys Pro Val 
    210                 215                 220                 


Val Ser Thr Gln Leu Leu Leu Asn Gly Ser Leu Ala Glu Glu Glu Val 
225                 230                 235                 240 


Met Ile Arg Ser Glu Asn Ile Thr Asn Asn Ala Lys Asn Ile Leu Val 
                245                 250                 255     


Gln Phe Asn Thr Pro Val Gln Ile Asn Cys Thr Arg Pro Asn Asn Asn 
            260                 265                 270         


Thr Arg Lys Ser Ile Arg Ile Gly Pro Gly Gln Ala Phe Tyr Ala Thr 
        275                 280                 285             


Gly Asp Ile Ile Gly Asp Ile Arg Gln Ala His Cys Asn Val Ser Lys 
    290                 295                 300                 


Ala Thr Trp Asn Glu Thr Leu Gly Lys Val Val Lys Gln Leu Arg Lys 
305                 310                 315                 320 


His Phe Gly Asn Asn Thr Ile Ile Arg Phe Ala Asn Ser Ser Gly Gly 
                325                 330                 335     


Asp Leu Glu Val Thr Thr His Ser Phe Asn Cys Gly Gly Glu Phe Phe 
            340                 345                 350         


Tyr Cys Asn Thr Ser Gly Leu Phe Asn Ser Thr Trp Ile Ser Asn Thr 
        355                 360                 365             


Ser Val Gln Gly Ser Asn Ser Thr Gly Ser Asn Asp Ser Ile Thr Leu 
    370                 375                 380                 


Pro Cys Arg Ile Lys Gln Ile Ile Asn Met Trp Gln Arg Ile Gly Gln 
385                 390                 395                 400 


Ala Met Tyr Ala Pro Pro Ile Gln Gly Val Ile Arg Cys Val Ser Asn 
                405                 410                 415     


Ile Thr Gly Leu Ile Leu Thr Arg Asp Gly Gly Ser Thr Asn Ser Thr 
            420                 425                 430         


Thr Glu Thr Phe Arg Pro Gly Gly Gly Asp Met Arg Asp Asn Trp Arg 
        435                 440                 445             


Ser Glu Leu Tyr Lys Tyr Lys Val Val Lys Ile Glu Pro Leu Gly Val 
    450                 455                 460                 


Ala Pro Thr Arg Cys Lys Arg Arg Val Val Gly Arg Arg Arg Arg Arg 
465                 470                 475                 480 


Arg Ala Val Gly Ile Gly Ala Val Phe Leu Gly Phe Leu Gly Ala Ala 
                485                 490                 495     


Gly Ser Thr Met Gly Ala Ala Ser Asn Thr Leu Thr Val Gln Ala Arg 
            500                 505                 510         


Asn Leu Leu Ser Gly Ile Val Gln Gln Gln Ser Asn Leu Pro Arg Ala 
        515                 520                 525             


Pro Glu Ala Gln Gln His Leu Leu Lys Leu Thr Val Trp Gly Ile Lys 
    530                 535                 540                 


Gln Leu Gln Ala Arg Val Leu Ala Val Glu Arg Tyr Leu Arg Val Gln 
545                 550                 555                 560 


Gln Leu Leu Gly Ile Trp Gly Cys Ser Gly Lys Leu Ile Cys Cys Thr 
                565                 570                 575     


Asn Val Pro Trp Asn Ser Ser Trp Ser Asn Arg Asn Leu Ser Glu Ile 
            580                 585                 590         


Trp Asp Asn Met Thr Trp Leu Gln Trp Asp Lys Glu Ile Ser Asn Tyr 
        595                 600                 605             


Thr Gln Ile Ile Tyr Gly Leu Leu Glu Glu Ser Gln Phe Gln Gln Glu 
    610                 615                 620                 


Ile Asn Glu Gln Asp Leu Leu Ala Leu Asp 
625                 630                 


<210>  23
<211>  619
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  wt C97ZA_SOSIP Env protein with L535M and Q567K (HIV150673)

<400>  23

Asn Met Trp Val Thr Val Tyr Tyr Gly Val Pro Val Trp Thr Asp Ala 
1               5                   10                  15      


Lys Thr Thr Leu Phe Cys Ala Ser Asp Thr Lys Ala Tyr Asp Arg Glu 
            20                  25                  30          


Val His Asn Val Trp Ala Thr His Ala Cys Val Pro Thr Asp Pro Asn 
        35                  40                  45              


Pro Gln Glu Ile Val Leu Glu Asn Val Thr Glu Asn Phe Asn Met Trp 
    50                  55                  60                  


Lys Asn Asp Met Val Asp Gln Met His Glu Asp Ile Ile Ser Leu Trp 
65                  70                  75                  80  


Asp Gln Ser Leu Lys Pro Cys Val Lys Leu Thr Pro Leu Cys Val Thr 
                85                  90                  95      


Leu His Cys Thr Asn Ala Thr Phe Lys Asn Asn Val Thr Asn Asp Met 
            100                 105                 110         


Asn Lys Glu Ile Arg Asn Cys Ser Phe Asn Thr Thr Thr Glu Ile Arg 
        115                 120                 125             


Asp Lys Lys Gln Gln Gly Tyr Ala Leu Phe Tyr Arg Pro Asp Ile Val 
    130                 135                 140                 


Leu Leu Lys Glu Asn Arg Asn Asn Ser Asn Asn Ser Glu Tyr Ile Leu 
145                 150                 155                 160 


Ile Asn Cys Asn Ala Ser Thr Ile Thr Gln Ala Cys Pro Lys Val Asn 
                165                 170                 175     


Phe Asp Pro Ile Pro Ile His Tyr Cys Ala Pro Ala Gly Tyr Ala Ile 
            180                 185                 190         


Leu Lys Cys Asn Asn Lys Thr Phe Ser Gly Lys Gly Pro Cys Asn Asn 
        195                 200                 205             


Val Ser Thr Val Gln Cys Thr His Gly Ile Lys Pro Val Val Ser Thr 
    210                 215                 220                 


Gln Leu Leu Leu Asn Gly Ser Leu Ala Glu Lys Glu Ile Ile Ile Arg 
225                 230                 235                 240 


Ser Glu Asn Leu Thr Asp Asn Val Lys Thr Ile Ile Val His Leu Asn 
                245                 250                 255     


Lys Ser Val Glu Ile Val Cys Thr Arg Pro Asn Asn Asn Thr Arg Lys 
            260                 265                 270         


Ser Met Arg Ile Gly Pro Gly Gln Thr Phe Tyr Ala Thr Gly Asp Ile 
        275                 280                 285             


Ile Gly Asp Ile Arg Gln Ala Tyr Cys Asn Ile Ser Gly Ser Lys Trp 
    290                 295                 300                 


Asn Glu Thr Leu Lys Arg Val Lys Glu Lys Leu Gln Glu Asn Tyr Asn 
305                 310                 315                 320 


Asn Asn Lys Thr Ile Lys Phe Ala Pro Ser Ser Gly Gly Asp Leu Glu 
                325                 330                 335     


Ile Thr Thr His Ser Phe Asn Cys Arg Gly Glu Phe Phe Tyr Cys Asn 
            340                 345                 350         


Thr Thr Arg Leu Phe Asn Asn Asn Ala Thr Glu Asp Glu Thr Ile Thr 
        355                 360                 365             


Leu Pro Cys Arg Ile Lys Gln Ile Ile Asn Met Trp Gln Gly Val Gly 
    370                 375                 380                 


Arg Ala Met Tyr Ala Pro Pro Ile Ala Gly Asn Ile Thr Cys Lys Ser 
385                 390                 395                 400 


Asn Ile Thr Gly Leu Leu Leu Val Arg Asp Gly Gly Glu Asp Asn Lys 
                405                 410                 415     


Thr Glu Glu Ile Phe Arg Pro Gly Gly Gly Asn Met Lys Asp Asn Trp 
            420                 425                 430         


Arg Ser Glu Leu Tyr Lys Tyr Lys Val Ile Glu Leu Lys Pro Leu Gly 
        435                 440                 445             


Ile Ala Pro Thr Gly Cys Lys Arg Arg Val Val Glu Arg Arg Arg Arg 
    450                 455                 460                 


Arg Arg Ala Val Gly Ile Gly Ala Val Phe Leu Gly Phe Leu Gly Ala 
465                 470                 475                 480 


Ala Gly Ser Thr Met Gly Ala Ala Ser Met Thr Leu Thr Val Gln Ala 
                485                 490                 495     


Arg Gln Leu Leu Ser Ser Ile Val Gln Gln Gln Ser Asn Leu Leu Arg 
            500                 505                 510         


Ala Pro Glu Ala Gln Gln His Met Leu Lys Leu Thr Val Trp Gly Ile 
        515                 520                 525             


Lys Gln Leu Gln Thr Arg Val Leu Ala Ile Glu Arg Tyr Leu Lys Asp 
    530                 535                 540                 


Gln Gln Leu Leu Gly Ile Trp Gly Cys Ser Gly Lys Leu Ile Cys Cys 
545                 550                 555                 560 


Thr Asn Val Pro Trp Asn Ser Ser Trp Ser Asn Lys Ser Gln Thr Asp 
                565                 570                 575     


Ile Trp Asn Asn Met Thr Trp Met Glu Trp Asp Arg Glu Ile Ser Asn 
            580                 585                 590         


Tyr Thr Asp Thr Ile Tyr Arg Leu Leu Glu Asp Ser Gln Thr Gln Gln 
        595                 600                 605             


Glu Lys Asn Glu Lys Asp Leu Leu Ala Leu Asp 
    610                 615                 


<210>  24
<211>  619
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  repaired and stabilized C97ZA_SOSIP Env protein (HIV170690)

<400>  24

Asn Leu Trp Val Thr Val Tyr Tyr Gly Val Pro Val Trp Lys Asp Ala 
1               5                   10                  15      


Lys Thr Thr Leu Phe Cys Ala Ser Asp Ala Lys Ala Tyr Asp Arg Glu 
            20                  25                  30          


Val His Asn Val Trp Ala Thr His Ala Cys Val Pro Thr Asp Pro Asn 
        35                  40                  45              


Pro Gln Glu Ile Val Leu Glu Asn Val Thr Glu Asn Phe Asn Met Trp 
    50                  55                  60                  


Lys Asn Asp Met Val Asp Gln Met His Glu Asp Ile Ile Ser Leu Trp 
65                  70                  75                  80  


Asp Gln Ser Leu Lys Pro Cys Val Lys Leu Thr Pro Leu Cys Val Thr 
                85                  90                  95      


Leu His Cys Thr Asn Ala Thr Phe Lys Asn Asn Val Thr Asn Asp Met 
            100                 105                 110         


Asn Lys Glu Ile Arg Asn Cys Ser Phe Asn Thr Thr Thr Glu Ile Arg 
        115                 120                 125             


Asp Lys Lys Gln Lys Val Tyr Ala Leu Phe Tyr Arg Leu Asp Ile Val 
    130                 135                 140                 


Gln Leu Lys Glu Asn Arg Asn Asn Ser Asn Asn Ser Glu Tyr Arg Leu 
145                 150                 155                 160 


Ile Asn Cys Asn Thr Ser Thr Ile Thr Gln Ile Cys Pro Lys Val Thr 
                165                 170                 175     


Phe Asp Pro Ile Pro Ile His Tyr Cys Ala Pro Ala Gly Tyr Ala Ile 
            180                 185                 190         


Leu Lys Cys Asn Asn Lys Thr Phe Asn Gly Thr Gly Pro Cys Asn Asn 
        195                 200                 205             


Val Ser Thr Val Gln Cys Thr His Gly Ile Lys Pro Val Val Ser Thr 
    210                 215                 220                 


Gln Leu Leu Leu Asn Gly Ser Leu Ala Glu Lys Glu Ile Ile Ile Arg 
225                 230                 235                 240 


Ser Glu Asn Leu Thr Asp Asn Val Lys Thr Ile Ile Val His Leu Asn 
                245                 250                 255     


Lys Ser Val Glu Ile Asn Cys Thr Arg Pro Asn Asn Asn Thr Arg Lys 
            260                 265                 270         


Ser Ile Arg Ile Gly Pro Gly Gln Thr Phe Tyr Ala Thr Gly Asp Ile 
        275                 280                 285             


Ile Gly Asp Ile Arg Gln Ala Tyr Cys Asn Ile Ser Gly Ser Lys Trp 
    290                 295                 300                 


Asn Glu Thr Leu Lys Arg Val Lys Glu Lys Leu Arg Glu His Phe Asn 
305                 310                 315                 320 


Asn Asn Lys Thr Ile Lys Phe Ala Pro Ser Ser Gly Gly Asp Leu Glu 
                325                 330                 335     


Ile Thr Thr His Ser Phe Asn Cys Arg Gly Glu Phe Phe Tyr Cys Asn 
            340                 345                 350         


Thr Thr Arg Leu Phe Asn Asn Asn Ala Thr Glu Asn Glu Thr Ile Thr 
        355                 360                 365             


Leu Pro Cys Arg Ile Lys Gln Ile Ile Asn Met Trp Gln Glu Val Gly 
    370                 375                 380                 


Arg Ala Met Tyr Ala Pro Pro Ile Ala Gly Asn Ile Thr Cys Lys Ser 
385                 390                 395                 400 


Asn Ile Thr Gly Leu Leu Leu Thr Arg Asp Gly Gly Glu Asp Asn Lys 
                405                 410                 415     


Thr Glu Glu Ile Phe Arg Pro Gly Gly Gly Asn Met Lys Asp Asn Trp 
            420                 425                 430         


Arg Ser Glu Leu Tyr Lys Tyr Lys Val Val Glu Ile Lys Pro Leu Gly 
        435                 440                 445             


Ile Ala Pro Thr Lys Cys Lys Arg Arg Asn Val Thr Arg Arg Arg Arg 
    450                 455                 460                 


Arg Arg Ala Val Gly Ile Gly Ala Val Phe Leu Gly Phe Leu Gly Ala 
465                 470                 475                 480 


Ala Gly Ser Thr Met Gly Ala Ala Ser Asn Thr Leu Thr Val Gln Ala 
                485                 490                 495     


Arg Gln Leu Leu Ser Gly Ile Val Gln Gln Gln Ser Asn Leu Pro Arg 
            500                 505                 510         


Ala Pro Glu Ala Gln Gln His Met Leu Lys Leu Thr Val Trp Gly Ile 
        515                 520                 525             


Lys Gln Leu Gln Ala Arg Val Leu Ala Ile Glu Arg Tyr Leu Glu Val 
    530                 535                 540                 


Gln Gln Leu Leu Gly Ile Trp Gly Cys Ser Gly Lys Leu Ile Cys Cys 
545                 550                 555                 560 


Thr Asn Val Pro Trp Asn Ser Ser Trp Ser Asn Lys Ser Gln Thr Asp 
                565                 570                 575     


Ile Trp Asn Asn Met Thr Trp Met Glu Trp Asp Arg Glu Ile Ser Asn 
            580                 585                 590         


Tyr Thr Asp Thr Ile Tyr Arg Leu Leu Glu Asp Ser Gln Phe Gln Gln 
        595                 600                 605             


Glu Ile Asn Glu Lys Asp Leu Leu Ala Asn Asp 
    610                 615                 


<210>  25
<211>  628
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  variant of repaired and stabilized Du422 construct (HIV161818)

<400>  25

Asn Leu Trp Val Thr Val Tyr Tyr Gly Val Pro Val Trp Lys Glu Ala 
1               5                   10                  15      


Lys Thr Thr Leu Phe Cys Ala Ser Asp Ala Lys Ala Tyr Asp Lys Glu 
            20                  25                  30          


Val His Asn Val Trp Ala Thr His Ala Cys Val Pro Thr Asp Pro Asn 
        35                  40                  45              


Pro Gln Glu Ile Val Leu Glu Asn Val Thr Glu Asn Phe Asn Met Trp 
    50                  55                  60                  


Lys Asn Asp Met Val Asp Gln Met His Glu Asp Ile Ile Ser Leu Trp 
65                  70                  75                  80  


Asp Gln Ser Leu Lys Pro Cys Val Lys Leu Thr Pro Leu Cys Val Thr 
                85                  90                  95      


Leu Asn Cys Lys Asn Val Asn Ile Ser Ala Asn Ala Asn Ala Thr Ala 
            100                 105                 110         


Thr Leu Asn Ser Ser Met Asn Gly Glu Ile Lys Asn Cys Ser Phe Asn 
        115                 120                 125             


Thr Thr Thr Glu Leu Arg Asp Lys Lys Gln Lys Val Tyr Ala Leu Phe 
    130                 135                 140                 


Tyr Lys Pro Asp Val Val Pro Leu Asn Gly Gly Glu His Asn Glu Thr 
145                 150                 155                 160 


Gly Glu Tyr Ile Leu Ile Asn Cys Asn Ser Ser Thr Cys Thr Gln Ala 
                165                 170                 175     


Cys Pro Lys Val Ser Phe Asp Pro Ile Pro Ile His Tyr Cys Ala Pro 
            180                 185                 190         


Ala Gly Tyr Ala Ile Leu Lys Cys Asn Asn Lys Thr Phe Asn Gly Thr 
        195                 200                 205             


Gly Pro Cys Asn Asn Val Ser Thr Val Gln Cys Thr His Gly Ile Lys 
    210                 215                 220                 


Pro Val Val Ser Thr Gln Leu Leu Leu Asn Gly Ser Leu Ala Glu Glu 
225                 230                 235                 240 


Glu Ile Ile Ile Arg Ser Glu Asn Leu Thr Asn Asn Ile Lys Thr Ile 
                245                 250                 255     


Ile Val His Leu Asn Lys Ser Val Glu Ile Asn Cys Thr Arg Pro Asn 
            260                 265                 270         


Asn Asn Thr Arg Lys Ser Val Arg Ile Gly Pro Gly Gln Thr Phe Tyr 
        275                 280                 285             


Ala Thr Gly Glu Ile Ile Gly Asp Ile Arg Glu Ala His Cys Asn Ile 
    290                 295                 300                 


Ser Arg Glu Thr Trp Asn Ser Thr Leu Ile Gln Val Lys Glu Lys Leu 
305                 310                 315                 320 


Arg Glu His Tyr Asn Lys Thr Ile Lys Phe Glu Pro Ser Ser Gly Gly 
                325                 330                 335     


Asp Leu Glu Val Thr Thr His Ser Phe Asn Cys Arg Gly Glu Phe Phe 
            340                 345                 350         


Tyr Cys Asn Thr Thr Lys Leu Phe Asn Glu Thr Lys Leu Phe Asn Glu 
        355                 360                 365             


Ser Glu Tyr Val Asp Asn Lys Thr Ile Ile Leu Pro Cys Arg Ile Lys 
    370                 375                 380                 


Gln Ile Ile Asn Met Trp Gln Glu Val Gly Arg Cys Met Tyr Ala Pro 
385                 390                 395                 400 


Pro Ile Glu Gly Asn Ile Thr Cys Lys Ser Asn Ile Thr Gly Leu Leu 
                405                 410                 415     


Leu Thr Arg Asp Gly Gly Glu Asn Ser Thr Glu Glu Val Phe Arg Pro 
            420                 425                 430         


Gly Gly Gly Asn Met Lys Asp Asn Trp Arg Ser Glu Leu Tyr Lys Tyr 
        435                 440                 445             


Lys Val Val Glu Ile Lys Pro Leu Gly Val Ala Pro Thr Lys Cys Lys 
    450                 455                 460                 


Arg Lys Asn Val Thr Arg Arg Arg Arg Arg Arg Ala Val Gly Leu Gly 
465                 470                 475                 480 


Ala Val Leu Leu Gly Phe Leu Gly Ala Ala Gly Ser Thr Met Gly Ala 
                485                 490                 495     


Ala Ser Asn Thr Leu Thr Val Gln Ala Arg Gln Leu Leu Ser Gly Ile 
            500                 505                 510         


Val Gln Gln Gln Ser Asn Leu Pro Arg Ala Pro Glu Ala Gln Gln His 
        515                 520                 525             


Leu Leu Gln Leu Thr Val Trp Gly Ile Lys Gln Leu Gln Thr Arg Val 
    530                 535                 540                 


Leu Ala Ile Glu Arg Tyr Leu Lys Val Gln Gln Leu Leu Gly Leu Trp 
545                 550                 555                 560 


Gly Cys Ser Gly Lys Leu Ile Cys Cys Thr Ala Val Pro Trp Asn Ser 
                565                 570                 575     


Ser Trp Ser Asn Lys Ser Leu Gly Asp Ile Trp Asp Asn Met Thr Trp 
            580                 585                 590         


Met Gln Trp Asp Arg Glu Ile Ser Asn Tyr Thr Asn Thr Ile Tyr Arg 
        595                 600                 605             


Leu Leu Glu Asp Ser Gln Phe Gln Gln Glu Lys Asn Glu Lys Asp Leu 
    610                 615                 620                 


Leu Ala Asn Asp 
625             


<210>  26
<211>  628
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  repaired and stabilized Du422_SOSIP Env protein (HIV170859)

<400>  26

Asn Leu Trp Val Thr Val Tyr Tyr Gly Val Pro Val Trp Lys Glu Ala 
1               5                   10                  15      


Lys Thr Thr Leu Phe Cys Ala Ser Asp Ala Lys Ala Tyr Asp Lys Glu 
            20                  25                  30          


Val His Asn Val Trp Ala Thr His Ala Cys Val Pro Thr Asp Pro Asn 
        35                  40                  45              


Pro Gln Glu Ile Val Leu Glu Asn Val Thr Glu Asn Phe Asn Met Trp 
    50                  55                  60                  


Lys Asn Asp Met Val Asp Gln Met His Glu Asp Ile Ile Ser Leu Trp 
65                  70                  75                  80  


Asp Gln Ser Leu Lys Pro Cys Val Lys Leu Thr Pro Leu Cys Val Thr 
                85                  90                  95      


Leu Asn Cys Lys Asn Val Asn Ile Ser Ala Asn Ala Asn Ala Thr Ala 
            100                 105                 110         


Thr Leu Asn Ser Ser Met Asn Gly Glu Ile Lys Asn Cys Ser Phe Asn 
        115                 120                 125             


Thr Thr Thr Glu Leu Arg Asp Lys Lys Gln Lys Val Tyr Ala Leu Phe 
    130                 135                 140                 


Tyr Lys Pro Asp Val Val Pro Leu Asn Gly Gly Glu His Asn Glu Thr 
145                 150                 155                 160 


Gly Glu Tyr Ile Leu Ile Asn Cys Asn Ser Ser Thr Ile Thr Gln Ala 
                165                 170                 175     


Cys Pro Lys Val Ser Phe Asp Pro Ile Pro Ile His Tyr Cys Ala Pro 
            180                 185                 190         


Ala Gly Tyr Ala Ile Leu Lys Cys Asn Asn Lys Thr Phe Asn Gly Thr 
        195                 200                 205             


Gly Pro Cys Asn Asn Val Ser Thr Val Gln Cys Thr His Gly Ile Lys 
    210                 215                 220                 


Pro Val Val Ser Thr Gln Leu Leu Leu Asn Gly Ser Leu Ala Glu Glu 
225                 230                 235                 240 


Glu Ile Ile Ile Arg Ser Glu Asn Leu Thr Asn Asn Ile Lys Thr Ile 
                245                 250                 255     


Ile Val His Leu Asn Lys Ser Val Glu Ile Asn Cys Thr Arg Pro Asn 
            260                 265                 270         


Asn Asn Thr Arg Lys Ser Val Arg Ile Gly Pro Gly Gln Thr Phe Tyr 
        275                 280                 285             


Ala Thr Gly Glu Ile Ile Gly Asp Ile Arg Glu Ala His Cys Asn Ile 
    290                 295                 300                 


Ser Arg Glu Thr Trp Asn Ser Thr Leu Ile Gln Val Lys Glu Lys Leu 
305                 310                 315                 320 


Arg Glu His Tyr Asn Lys Thr Ile Lys Phe Glu Pro Ser Ser Gly Gly 
                325                 330                 335     


Asp Leu Glu Val Thr Thr His Ser Phe Asn Cys Arg Gly Glu Phe Phe 
            340                 345                 350         


Tyr Cys Asn Thr Thr Lys Leu Phe Asn Glu Thr Lys Leu Phe Asn Glu 
        355                 360                 365             


Ser Glu Tyr Val Asp Asn Lys Thr Ile Ile Leu Pro Cys Arg Ile Lys 
    370                 375                 380                 


Gln Ile Ile Asn Met Trp Gln Glu Val Gly Arg Ala Met Tyr Ala Pro 
385                 390                 395                 400 


Pro Ile Glu Gly Asn Ile Thr Cys Lys Ser Asn Ile Thr Gly Leu Leu 
                405                 410                 415     


Leu Thr Arg Asp Gly Gly Glu Asn Ser Thr Glu Glu Val Phe Arg Pro 
            420                 425                 430         


Gly Gly Gly Asn Met Lys Asp Asn Trp Arg Ser Glu Leu Tyr Lys Tyr 
        435                 440                 445             


Lys Val Val Glu Ile Lys Pro Leu Gly Val Ala Pro Thr Lys Cys Lys 
    450                 455                 460                 


Arg Lys Val Val Gly Arg Arg Arg Arg Arg Arg Ala Val Gly Leu Gly 
465                 470                 475                 480 


Ala Val Leu Leu Gly Phe Leu Gly Ala Ala Gly Ser Thr Met Gly Ala 
                485                 490                 495     


Ala Ser Asn Thr Leu Thr Val Gln Ala Arg Gln Leu Leu Ser Gly Ile 
            500                 505                 510         


Val Gln Gln Gln Ser Asn Leu Pro Arg Ala Pro Glu Ala Gln Gln His 
        515                 520                 525             


Leu Leu Gln Leu Thr Val Trp Gly Ile Lys Gln Leu Gln Thr Arg Val 
    530                 535                 540                 


Leu Ala Ile Glu Arg Tyr Leu Glu Val Gln Gln Leu Leu Gly Leu Trp 
545                 550                 555                 560 


Gly Cys Ser Gly Lys Leu Ile Cys Cys Thr Ala Val Pro Trp Asn Ser 
                565                 570                 575     


Ser Trp Ser Asn Lys Ser Leu Gly Asp Ile Trp Asp Asn Met Thr Trp 
            580                 585                 590         


Met Gln Trp Asp Arg Glu Ile Ser Asn Tyr Thr Asn Thr Ile Tyr Arg 
        595                 600                 605             


Leu Leu Glu Asp Ser Gln Phe Gln Gln Glu Ile Asn Glu Lys Asp Leu 
    610                 615                 620                 


Leu Ala Leu Asp 
625             


<210>  27
<211>  599
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  repaired and stabilized DS_sC4_SOSIP Env protein (HIV170686)

<400>  27

Met Gly Asn Leu Trp Val Thr Val Tyr Tyr Gly Val Pro Val Trp Lys 
1               5                   10                  15      


Asp Ala Lys Thr Thr Leu Phe Cys Ala Ser Asp Ala Lys Ala Tyr Glu 
            20                  25                  30          


Lys Glu Val His Asn Val Trp Ala Thr His Ala Cys Val Pro Thr Asp 
        35                  40                  45              


Pro Asn Pro Gln Glu Ile Val Leu Gly Asn Val Thr Glu Asn Phe Asn 
    50                  55                  60                  


Met Trp Lys Asn Asp Met Val Asp Gln Met His Glu Asp Ile Ile Ser 
65                  70                  75                  80  


Leu Trp Asp Gln Ser Leu Lys Pro Cys Val Lys Leu Thr Pro Leu Cys 
                85                  90                  95      


Val Thr Leu Asn Cys Arg Asn Val Arg Asn Val Glu Met Lys Asn Cys 
            100                 105                 110         


Ser Phe Asn Ala Thr Thr Val Val Arg Asp Arg Lys Gln Lys Val His 
        115                 120                 125             


Ala Leu Phe Tyr Arg Leu Asp Ile Val Pro Leu Asp Glu Asn Asn Ser 
    130                 135                 140                 


Ser Tyr Arg Leu Ile Asn Cys Asn Thr Ser Ala Cys Thr Gln Ile Cys 
145                 150                 155                 160 


Pro Lys Val Ser Phe Asp Pro Ile Pro Ile His Tyr Cys Ala Pro Ala 
                165                 170                 175     


Gly Tyr Ala Ile Leu Lys Cys Asn Asn Lys Thr Phe Asn Gly Thr Gly 
            180                 185                 190         


Pro Cys Asn Asn Val Ser Thr Val Gln Cys Thr His Gly Ile Lys Pro 
        195                 200                 205             


Val Val Ser Thr Gln Leu Leu Leu Asn Gly Ser Leu Ala Glu Glu Glu 
    210                 215                 220                 


Ile Ile Ile Arg Ser Glu Asn Leu Thr Asn Asn Ala Lys Thr Ile Ile 
225                 230                 235                 240 


Val His Leu Asn Glu Thr Val Asn Ile Asn Cys Thr Arg Pro Asn Asn 
                245                 250                 255     


Asn Thr Arg Lys Ser Ile Arg Ile Gly Pro Gly Gln Thr Phe Tyr Ala 
            260                 265                 270         


Thr Gly Asp Ile Ile Gly Asp Ile Arg Gln Ala His Cys Asn Leu Ser 
        275                 280                 285             


Arg Asp Gly Trp Asn Lys Thr Leu Gln Gly Val Lys Lys Lys Leu Ala 
    290                 295                 300                 


Glu His Phe Pro Asn Lys Thr Ile Lys Phe Ala Pro His Ser Gly Gly 
305                 310                 315                 320 


Asp Leu Glu Ile Thr Thr His Ser Phe Asn Cys Arg Gly Glu Phe Phe 
                325                 330                 335     


Tyr Cys Asn Thr Ser Asn Leu Phe Asn Glu Ser Asn Ile Glu Arg Asn 
            340                 345                 350         


Asp Ser Ile Ile Thr Leu Pro Cys Arg Ile Lys Gln Ile Ile Asn Met 
        355                 360                 365             


Trp Gln Glu Val Gly Arg Cys Met Tyr Ala Pro Pro Ile Ala Gly Asn 
    370                 375                 380                 


Ile Thr Cys Arg Ser Asn Ile Thr Gly Leu Leu Leu Thr Arg Asp Gly 
385                 390                 395                 400 


Gly Ser Asn Asn Asn Asp Thr Glu Thr Phe Arg Pro Gly Gly Gly Asp 
                405                 410                 415     


Met Arg Asn Asn Trp Arg Ser Glu Leu Tyr Lys Tyr Lys Val Val Glu 
            420                 425                 430         


Val Lys Pro Leu Gly Val Ala Pro Thr Glu Cys Lys Arg Arg Val Val 
        435                 440                 445             


Glu Arg Arg Arg Arg Arg Arg Ala Val Gly Ile Gly Ala Val Phe Leu 
    450                 455                 460                 


Gly Ile Leu Gly Ala Ala Gly Ser Thr Met Gly Ala Ala Ser Asn Thr 
465                 470                 475                 480 


Leu Thr Val Gln Ala Arg Gln Leu Leu Ser Gly Ile Val Gln Gln Gln 
                485                 490                 495     


Ser Asn Leu Pro Arg Ala Pro Glu Ala Gln Gln His Met Leu Gln Leu 
            500                 505                 510         


Thr Val Trp Gly Ile Lys Gln Leu Gln Thr Arg Val Leu Ala Ile Glu 
        515                 520                 525             


Arg Tyr Leu Glu Val Gln Gln Leu Leu Gly Leu Trp Gly Cys Ser Gly 
    530                 535                 540                 


Lys Leu Ile Cys Cys Thr Ala Val Pro Trp Asn Thr Ser Trp Ser Asn 
545                 550                 555                 560 


Lys Ser Gln Thr Asp Ile Trp Asp Asn Met Thr Trp Met Gln Trp Asp 
                565                 570                 575     


Lys Glu Ile Gly Asn Tyr Thr Gly Glu Ile Tyr Arg Leu Leu Glu Glu 
            580                 585                 590         


Ser Gln Phe Gln Gln Glu Ile 
        595                 


<210>  28
<211>  607
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Stabilized ConC_SOSIP.v3 Env protein (HIV170654)

<400>  28

Asn Leu Trp Val Thr Val Tyr Tyr Gly Val Pro Val Trp Lys Glu Ala 
1               5                   10                  15      


Lys Thr Thr Leu Phe Cys Ala Ser Asp Ala Lys Ala Tyr Glu Lys Glu 
            20                  25                  30          


Val His Asn Val Trp Ala Thr His Ala Cys Val Pro Thr Asp Pro Asn 
        35                  40                  45              


Pro Gln Glu Met Val Leu Glu Asn Val Thr Glu Asn Phe Asn Met Trp 
    50                  55                  60                  


Lys Asn Asp Met Val Asp Gln Met His Glu Asp Ile Ile Ser Leu Trp 
65                  70                  75                  80  


Asp Gln Ser Leu Lys Pro Cys Val Lys Leu Thr Pro Leu Cys Val Thr 
                85                  90                  95      


Leu Asn Cys Thr Asn Val Asn Val Thr Glu Met Lys Asn Cys Ser Phe 
            100                 105                 110         


Asn Thr Thr Thr Glu Ile Arg Asp Lys Lys Gln Lys Glu Tyr Ala Leu 
        115                 120                 125             


Phe Tyr Arg Leu Asp Ile Val Pro Leu Asn Glu Asn Ser Ser Glu Tyr 
    130                 135                 140                 


Arg Leu Ile Asn Cys Asn Thr Ser Thr Ile Thr Gln Ile Cys Pro Lys 
145                 150                 155                 160 


Val Ser Phe Asp Pro Ile Pro Ile His Tyr Cys Ala Pro Ala Gly Tyr 
                165                 170                 175     


Ala Ile Leu Lys Cys Asn Asn Lys Thr Phe Asn Gly Thr Gly Pro Cys 
            180                 185                 190         


Asn Asn Val Ser Thr Val Gln Cys Thr His Gly Ile Lys Pro Val Val 
        195                 200                 205             


Ser Thr Gln Leu Leu Leu Asn Gly Ser Leu Ala Glu Glu Glu Ile Ile 
    210                 215                 220                 


Ile Arg Ser Glu Asn Leu Thr Asn Asn Val Lys Thr Ile Ile Val His 
225                 230                 235                 240 


Leu Asn Glu Ser Val Glu Ile Val Cys Thr Arg Pro Asn Asn Asn Thr 
                245                 250                 255     


Arg Lys Ser Ile Arg Ile Gly Pro Gly Gln Thr Phe Tyr Ala Thr Gly 
            260                 265                 270         


Asp Ile Ile Gly Asp Ile Arg Gln Ala His Cys Asn Ile Ser Glu Ala 
        275                 280                 285             


Lys Trp Asn Lys Thr Leu Gln Arg Val Lys Lys Lys Leu Lys Glu His 
    290                 295                 300                 


Phe Pro Asn Lys Thr Ile Lys Phe Gln Pro Ser Ser Gly Gly Asp Leu 
305                 310                 315                 320 


Glu Ile Thr Thr His Ser Phe Asn Cys Arg Gly Glu Phe Phe Tyr Cys 
                325                 330                 335     


Asn Thr Ser Lys Leu Phe Asn Ser Thr Tyr Asn Asn Thr Thr Ser Asn 
            340                 345                 350         


Ser Thr Ile Thr Leu Pro Cys Arg Ile Lys Gln Ile Ile Asn Met Trp 
        355                 360                 365             


Gln Glu Val Gly Arg Ala Met Tyr Ala Pro Pro Ile Ala Gly Asn Ile 
    370                 375                 380                 


Thr Cys Lys Ser Asn Ile Thr Gly Leu Leu Leu Thr Arg Asp Gly Gly 
385                 390                 395                 400 


Asn Asn Asn Asn Asn Thr Glu Thr Phe Arg Pro Gly Gly Gly Asp Met 
                405                 410                 415     


Arg Asp Asn Trp Arg Ser Glu Leu Tyr Lys Tyr Lys Val Val Glu Ile 
            420                 425                 430         


Lys Pro Leu Gly Ile Ala Pro Thr Lys Cys Lys Arg Arg Val Val Glu 
        435                 440                 445             


Arg Arg Arg Arg Arg Arg Ala Val Gly Ile Gly Ala Val Phe Leu Gly 
    450                 455                 460                 


Phe Leu Gly Ala Ala Gly Ser Thr Met Gly Ala Ala Ser Asn Thr Leu 
465                 470                 475                 480 


Thr Val Gln Ala Arg Gln Leu Leu Ser Gly Ile Val Gln Gln Gln Ser 
                485                 490                 495     


Asn Leu Leu Arg Ala Pro Glu Ala Gln Gln His Met Leu Gln Leu Thr 
            500                 505                 510         


Val Trp Gly Phe Lys Gln Leu Gln Ala Arg Val Leu Ala Ile Glu Arg 
        515                 520                 525             


Tyr Leu Glu Val Gln Gln Leu Leu Gly Ile Trp Gly Cys Ser Gly Lys 
    530                 535                 540                 


Leu Ile Cys Cys Thr Ala Val Pro Trp Asn Ser Ser Trp Ser Asn Lys 
545                 550                 555                 560 


Ser Gln Glu Asp Ile Trp Asp Asn Met Thr Trp Met Gln Trp Asp Arg 
                565                 570                 575     


Glu Ile Ser Asn Tyr Thr Asp Thr Ile Tyr Arg Leu Leu Glu Glu Ser 
            580                 585                 590         


Gln Phe Gln Gln Glu Ile Asn Glu Lys Asp Leu Leu Ala Leu Asp 
        595                 600                 605         


<210>  29
<211>  634
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Stabilized BG505_SOSIP.v2 Env protein (HIV171814)

<400>  29

Ala Glu Asn Leu Trp Val Thr Val Tyr Tyr Gly Val Pro Val Trp Lys 
1               5                   10                  15      


Asp Ala Glu Thr Thr Leu Phe Cys Ala Ser Asp Ala Lys Ala Tyr Glu 
            20                  25                  30          


Thr Glu Lys His Asn Val Trp Ala Thr His Ala Cys Val Pro Thr Asp 
        35                  40                  45              


Pro Asn Pro Gln Glu Ile His Leu Glu Asn Val Thr Glu Glu Phe Asn 
    50                  55                  60                  


Met Trp Lys Asn Asn Met Val Glu Gln Met His Thr Asp Ile Ile Ser 
65                  70                  75                  80  


Leu Trp Asp Gln Ser Leu Lys Pro Cys Val Lys Leu Thr Pro Leu Cys 
                85                  90                  95      


Val Thr Leu Gln Cys Thr Asn Val Thr Asn Asn Ile Thr Asp Asp Met 
            100                 105                 110         


Arg Gly Glu Leu Lys Asn Cys Ser Phe Asn Met Thr Thr Glu Leu Arg 
        115                 120                 125             


Asp Lys Lys Gln Lys Val Tyr Ser Leu Phe Tyr Arg Leu Asp Val Val 
    130                 135                 140                 


Gln Ile Asn Glu Asn Gln Gly Asn Arg Ser Asn Asn Ser Asn Lys Glu 
145                 150                 155                 160 


Tyr Arg Leu Ile Asn Cys Asn Thr Ser Ala Cys Thr Gln Ala Cys Pro 
                165                 170                 175     


Lys Val Ser Phe Glu Pro Ile Pro Ile His Tyr Cys Ala Pro Ala Gly 
            180                 185                 190         


Phe Ala Ile Leu Lys Cys Lys Asp Lys Lys Phe Asn Gly Thr Gly Pro 
        195                 200                 205             


Cys Pro Ser Val Ser Thr Val Gln Cys Thr His Gly Ile Lys Pro Val 
    210                 215                 220                 


Val Ser Thr Gln Leu Leu Leu Asn Gly Ser Leu Ala Glu Glu Glu Val 
225                 230                 235                 240 


Met Ile Arg Ser Glu Asn Ile Thr Asn Asn Ala Lys Asn Ile Leu Val 
                245                 250                 255     


Gln Phe Asn Thr Pro Val Gln Ile Asn Cys Thr Arg Pro Asn Asn Asn 
            260                 265                 270         


Thr Arg Lys Ser Ile Arg Ile Gly Pro Gly Gln Ala Phe Tyr Ala Thr 
        275                 280                 285             


Gly Asp Ile Ile Gly Asp Ile Arg Gln Ala His Cys Asn Val Ser Lys 
    290                 295                 300                 


Ala Thr Trp Asn Glu Thr Leu Gly Lys Val Val Lys Gln Leu Arg Lys 
305                 310                 315                 320 


His Phe Gly Asn Asn Thr Ile Ile Arg Phe Ala Asn Ser Ser Gly Gly 
                325                 330                 335     


Asp Leu Glu Val Thr Thr His Ser Phe Asn Cys Gly Gly Glu Phe Phe 
            340                 345                 350         


Tyr Cys Asn Thr Ser Gly Leu Phe Asn Ser Thr Trp Ile Ser Asn Thr 
        355                 360                 365             


Ser Val Gln Gly Ser Asn Ser Thr Gly Ser Asn Asp Ser Ile Thr Leu 
    370                 375                 380                 


Pro Cys Arg Ile Lys Gln Ile Ile Asn Met Trp Gln Arg Ile Gly Gln 
385                 390                 395                 400 


Cys Met Tyr Ala Pro Pro Ile Gln Gly Val Ile Arg Cys Val Ser Asn 
                405                 410                 415     


Ile Thr Gly Leu Ile Leu Thr Arg Asp Gly Gly Ser Thr Asn Ser Thr 
            420                 425                 430         


Thr Glu Thr Phe Arg Pro Gly Gly Gly Asp Met Arg Asp Asn Trp Arg 
        435                 440                 445             


Ser Glu Leu Tyr Lys Tyr Lys Val Val Lys Ile Glu Pro Leu Gly Val 
    450                 455                 460                 


Ala Pro Thr Arg Cys Lys Arg Arg Val Val Gly Arg Arg Arg Arg Arg 
465                 470                 475                 480 


Arg Ala Val Gly Ile Gly Ala Val Phe Leu Gly Phe Leu Gly Ala Ala 
                485                 490                 495     


Gly Ser Thr Met Gly Ala Ala Ser Asn Thr Leu Thr Val Gln Ala Arg 
            500                 505                 510         


Asn Leu Leu Ser Gly Ile Val Gln Gln Gln Ser Asn Leu Pro Arg Ala 
        515                 520                 525             


Pro Glu Ala Gln Gln His Leu Leu Lys Leu Thr Val Trp Gly Ile Lys 
    530                 535                 540                 


Gln Leu Gln Ala Arg Val Leu Ala Val Glu Arg Tyr Leu Glu Val Gln 
545                 550                 555                 560 


Gln Leu Leu Gly Ile Trp Gly Cys Ser Gly Lys Leu Ile Cys Cys Thr 
                565                 570                 575     


Asn Val Pro Trp Asn Ser Ser Trp Ser Asn Arg Asn Leu Ser Glu Ile 
            580                 585                 590         


Trp Asp Asn Met Thr Trp Leu Gln Trp Asp Lys Glu Ile Ser Asn Tyr 
        595                 600                 605             


Thr Gln Ile Ile Tyr Gly Leu Leu Glu Glu Ser Gln Phe Gln Gln Glu 
    610                 615                 620                 


Ile Asn Glu Val Asp Leu Leu Ala Leu Asp 
625                 630                 


<210>  30
<211>  619
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Repaired and stabilized C97ZA_SOSIP.v2 Env protein (HIV171810)

<400>  30

Asn Leu Trp Val Thr Val Tyr Tyr Gly Val Pro Val Trp Lys Asp Ala 
1               5                   10                  15      


Lys Thr Thr Leu Phe Cys Ala Ser Asp Ala Lys Ala Tyr Asp Arg Glu 
            20                  25                  30          


Val His Asn Val Trp Ala Thr His Ala Cys Val Pro Thr Asp Pro Asn 
        35                  40                  45              


Pro Gln Glu Ile Val Leu Glu Asn Val Thr Glu Asn Phe Asn Met Trp 
    50                  55                  60                  


Lys Asn Asp Met Val Asp Gln Met His Glu Asp Ile Ile Ser Leu Trp 
65                  70                  75                  80  


Asp Gln Ser Leu Lys Pro Cys Val Lys Leu Thr Pro Leu Cys Val Thr 
                85                  90                  95      


Leu His Cys Thr Asn Ala Thr Phe Lys Asn Asn Val Thr Asn Asp Met 
            100                 105                 110         


Asn Lys Glu Ile Arg Asn Cys Ser Phe Asn Thr Thr Thr Glu Ile Arg 
        115                 120                 125             


Asp Lys Lys Gln Lys Val Tyr Ala Leu Phe Tyr Arg Leu Asp Ile Val 
    130                 135                 140                 


Gln Leu Lys Glu Asn Arg Asn Asn Ser Asn Asn Ser Glu Tyr Arg Leu 
145                 150                 155                 160 


Ile Asn Cys Asn Thr Ser Thr Cys Thr Gln Ile Cys Pro Lys Val Thr 
                165                 170                 175     


Phe Asp Pro Ile Pro Ile His Tyr Cys Ala Pro Ala Gly Tyr Ala Ile 
            180                 185                 190         


Leu Lys Cys Asn Asn Lys Thr Phe Asn Gly Thr Gly Pro Cys Asn Asn 
        195                 200                 205             


Val Ser Thr Val Gln Cys Thr His Gly Ile Lys Pro Val Val Ser Thr 
    210                 215                 220                 


Gln Leu Leu Leu Asn Gly Ser Leu Ala Glu Lys Glu Ile Ile Ile Arg 
225                 230                 235                 240 


Ser Glu Asn Leu Thr Asp Asn Val Lys Thr Ile Ile Val His Leu Asn 
                245                 250                 255     


Lys Ser Val Glu Ile Asn Cys Thr Arg Pro Asn Asn Asn Thr Arg Lys 
            260                 265                 270         


Ser Ile Arg Ile Gly Pro Gly Gln Thr Phe Tyr Ala Thr Gly Asp Ile 
        275                 280                 285             


Ile Gly Asp Ile Arg Gln Ala Tyr Cys Asn Ile Ser Gly Ser Lys Trp 
    290                 295                 300                 


Asn Glu Thr Leu Lys Arg Val Lys Glu Lys Leu Arg Glu His Phe Asn 
305                 310                 315                 320 


Asn Asn Lys Thr Ile Lys Phe Ala Pro Ser Ser Gly Gly Asp Leu Glu 
                325                 330                 335     


Ile Thr Thr His Ser Phe Asn Cys Arg Gly Glu Phe Phe Tyr Cys Asn 
            340                 345                 350         


Thr Thr Arg Leu Phe Asn Asn Asn Ala Thr Glu Asn Glu Thr Ile Thr 
        355                 360                 365             


Leu Pro Cys Arg Ile Lys Gln Ile Ile Asn Met Trp Gln Glu Val Gly 
    370                 375                 380                 


Arg Cys Met Tyr Ala Pro Pro Ile Ala Gly Asn Ile Thr Cys Lys Ser 
385                 390                 395                 400 


Asn Ile Thr Gly Leu Leu Leu Thr Arg Asp Gly Gly Glu Asp Asn Lys 
                405                 410                 415     


Thr Glu Glu Ile Phe Arg Pro Gly Gly Gly Asn Met Lys Asp Asn Trp 
            420                 425                 430         


Arg Ser Glu Leu Tyr Lys Tyr Lys Val Val Glu Ile Lys Pro Leu Gly 
        435                 440                 445             


Ile Ala Pro Thr Lys Cys Lys Arg Arg Asn Val Thr Arg Arg Arg Arg 
    450                 455                 460                 


Arg Arg Ala Val Gly Ile Gly Ala Val Phe Leu Gly Phe Leu Gly Ala 
465                 470                 475                 480 


Ala Gly Ser Thr Met Gly Ala Ala Ser Asn Thr Leu Thr Val Gln Ala 
                485                 490                 495     


Arg Gln Leu Leu Ser Gly Ile Val Gln Gln Gln Ser Asn Leu Pro Arg 
            500                 505                 510         


Ala Pro Glu Ala Gln Gln His Met Leu Lys Leu Thr Val Trp Gly Ile 
        515                 520                 525             


Lys Gln Leu Gln Ala Arg Val Leu Ala Ile Glu Arg Tyr Leu Glu Val 
    530                 535                 540                 


Gln Gln Leu Leu Gly Ile Trp Gly Cys Ser Gly Lys Leu Ile Cys Cys 
545                 550                 555                 560 


Thr Asn Val Pro Trp Asn Ser Ser Trp Ser Asn Lys Ser Gln Thr Asp 
                565                 570                 575     


Ile Trp Asn Asn Met Thr Trp Met Glu Trp Asp Arg Glu Ile Ser Asn 
            580                 585                 590         


Tyr Thr Asp Thr Ile Tyr Arg Leu Leu Glu Asp Ser Gln Phe Gln Gln 
        595                 600                 605             


Glu Ile Asn Glu Val Asp Leu Leu Ala Asn Asp 
    610                 615                 


<210>  31
<211>  628
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Repaired and stabilized Du422_SOSIP.v1 Env protein (HIV171812)

<400>  31

Asn Leu Trp Val Thr Val Tyr Tyr Gly Val Pro Val Trp Lys Glu Ala 
1               5                   10                  15      


Lys Thr Thr Leu Phe Cys Ala Ser Asp Ala Lys Ala Tyr Asp Lys Glu 
            20                  25                  30          


Val His Asn Val Trp Ala Thr His Ala Cys Val Pro Thr Asp Pro Asn 
        35                  40                  45              


Pro Gln Glu Ile Val Leu Glu Asn Val Thr Glu Asn Phe Asn Met Trp 
    50                  55                  60                  


Lys Asn Asp Met Val Asp Gln Met His Glu Asp Ile Ile Ser Leu Trp 
65                  70                  75                  80  


Asp Gln Ser Leu Lys Pro Cys Val Lys Leu Thr Pro Leu Cys Val Thr 
                85                  90                  95      


Leu Asn Cys Lys Asn Val Asn Ile Ser Ala Asn Ala Asn Ala Thr Ala 
            100                 105                 110         


Thr Leu Asn Ser Ser Met Asn Gly Glu Ile Lys Asn Cys Ser Phe Asn 
        115                 120                 125             


Thr Thr Thr Glu Leu Arg Asp Lys Lys Gln Lys Val Tyr Ala Leu Phe 
    130                 135                 140                 


Tyr Lys Pro Asp Val Val Pro Leu Asn Gly Gly Glu His Asn Glu Thr 
145                 150                 155                 160 


Gly Glu Tyr Ile Leu Ile Asn Cys Asn Ser Ser Thr Cys Thr Gln Ala 
                165                 170                 175     


Cys Pro Lys Val Ser Phe Asp Pro Ile Pro Ile His Tyr Cys Ala Pro 
            180                 185                 190         


Ala Gly Tyr Ala Ile Leu Lys Cys Asn Asn Lys Thr Phe Asn Gly Thr 
        195                 200                 205             


Gly Pro Cys Asn Asn Val Ser Thr Val Gln Cys Thr His Gly Ile Lys 
    210                 215                 220                 


Pro Val Val Ser Thr Gln Leu Leu Leu Asn Gly Ser Leu Ala Glu Glu 
225                 230                 235                 240 


Glu Ile Ile Ile Arg Ser Glu Asn Leu Thr Asn Asn Ile Lys Thr Ile 
                245                 250                 255     


Ile Val His Leu Asn Lys Ser Val Glu Ile Asn Cys Thr Arg Pro Asn 
            260                 265                 270         


Asn Asn Thr Arg Lys Ser Val Arg Ile Gly Pro Gly Gln Thr Phe Tyr 
        275                 280                 285             


Ala Thr Gly Glu Ile Ile Gly Asp Ile Arg Glu Ala His Cys Asn Ile 
    290                 295                 300                 


Ser Arg Glu Thr Trp Asn Ser Thr Leu Ile Gln Val Lys Glu Lys Leu 
305                 310                 315                 320 


Arg Glu His Tyr Asn Lys Thr Ile Lys Phe Glu Pro Ser Ser Gly Gly 
                325                 330                 335     


Asp Leu Glu Val Thr Thr His Ser Phe Asn Cys Arg Gly Glu Phe Phe 
            340                 345                 350         


Tyr Cys Asn Thr Thr Lys Leu Phe Asn Glu Thr Lys Leu Phe Asn Glu 
        355                 360                 365             


Ser Glu Tyr Val Asp Asn Lys Thr Ile Ile Leu Pro Cys Arg Ile Lys 
    370                 375                 380                 


Gln Ile Ile Asn Met Trp Gln Glu Val Gly Arg Cys Met Tyr Ala Pro 
385                 390                 395                 400 


Pro Ile Glu Gly Asn Ile Thr Cys Lys Ser Asn Ile Thr Gly Leu Leu 
                405                 410                 415     


Leu Thr Arg Asp Gly Gly Glu Asn Ser Thr Glu Glu Val Phe Arg Pro 
            420                 425                 430         


Gly Gly Gly Asn Met Lys Asp Asn Trp Arg Ser Glu Leu Tyr Lys Tyr 
        435                 440                 445             


Lys Val Val Glu Ile Lys Pro Leu Gly Val Ala Pro Thr Lys Cys Lys 
    450                 455                 460                 


Arg Lys Val Val Gly Arg Arg Arg Arg Arg Arg Ala Val Gly Leu Gly 
465                 470                 475                 480 


Ala Val Leu Leu Gly Phe Leu Gly Ala Ala Gly Ser Thr Met Gly Ala 
                485                 490                 495     


Ala Ser Asn Thr Leu Thr Val Gln Ala Arg Gln Leu Leu Ser Gly Ile 
            500                 505                 510         


Val Gln Gln Gln Ser Asn Leu Pro Arg Ala Pro Glu Ala Gln Gln His 
        515                 520                 525             


Leu Leu Gln Leu Thr Val Trp Gly Ile Lys Gln Leu Gln Thr Arg Val 
    530                 535                 540                 


Leu Ala Ile Glu Arg Tyr Leu Glu Val Gln Gln Leu Leu Gly Leu Trp 
545                 550                 555                 560 


Gly Cys Ser Gly Lys Leu Ile Cys Cys Thr Ala Val Pro Trp Asn Ser 
                565                 570                 575     


Ser Trp Ser Asn Lys Ser Leu Gly Asp Ile Trp Asp Asn Met Thr Trp 
            580                 585                 590         


Met Gln Trp Asp Arg Glu Ile Ser Asn Tyr Thr Asn Thr Ile Tyr Arg 
        595                 600                 605             


Leu Leu Glu Asp Ser Gln Phe Gln Gln Glu Ile Asn Glu Val Asp Leu 
    610                 615                 620                 


Leu Ala Leu Asp 
625             


<210>  32
<211>  599
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Stabilized and repaired sC4_SOSIP.v4 Env protein

<400>  32

Met Gly Asn Leu Trp Val Thr Val Tyr Tyr Gly Val Pro Val Trp Lys 
1               5                   10                  15      


Asp Ala Lys Thr Thr Leu Phe Cys Ala Ser Asp Ala Lys Ala Tyr Glu 
            20                  25                  30          


Lys Glu Val His Asn Val Trp Ala Thr His Ala Cys Val Pro Thr Asp 
        35                  40                  45              


Pro Asn Pro Gln Glu Ile Val Leu Gly Asn Val Thr Glu Asn Phe Asn 
    50                  55                  60                  


Met Trp Lys Asn Asp Met Val Asp Gln Met His Glu Asp Ile Ile Ser 
65                  70                  75                  80  


Leu Trp Asp Gln Ser Leu Lys Pro Cys Val Lys Leu Thr Pro Leu Cys 
                85                  90                  95      


Val Thr Leu Asn Cys Arg Asn Val Arg Asn Val Glu Met Lys Asn Cys 
            100                 105                 110         


Ser Phe Asn Ala Thr Thr Val Val Arg Asp Arg Lys Gln Lys Val His 
        115                 120                 125             


Ala Leu Phe Tyr Arg Leu Asp Ile Val Pro Leu Asp Glu Asn Asn Ser 
    130                 135                 140                 


Ser Tyr Arg Leu Ile Asn Cys Asn Thr Ser Ala Cys Thr Gln Ile Cys 
145                 150                 155                 160 


Pro Lys Val Ser Phe Asp Pro Ile Pro Ile His Tyr Cys Ala Pro Ala 
                165                 170                 175     


Gly Tyr Ala Ile Leu Lys Cys Asn Asn Lys Thr Phe Asn Gly Thr Gly 
            180                 185                 190         


Pro Cys Asn Asn Val Ser Thr Val Gln Cys Thr His Gly Ile Lys Pro 
        195                 200                 205             


Val Val Ser Thr Gln Leu Leu Leu Asn Gly Ser Leu Ala Glu Glu Glu 
    210                 215                 220                 


Ile Ile Ile Arg Ser Glu Asn Leu Thr Asn Asn Ala Lys Thr Ile Ile 
225                 230                 235                 240 


Val His Leu Asn Glu Thr Val Asn Ile Val Cys Thr Arg Pro Asn Asn 
                245                 250                 255     


Asn Thr Arg Lys Ser Ile Arg Ile Gly Pro Gly Gln Thr Phe Tyr Ala 
            260                 265                 270         


Thr Gly Asp Ile Ile Gly Asp Ile Arg Gln Ala His Cys Asn Leu Ser 
        275                 280                 285             


Arg Asp Gly Trp Asn Lys Thr Leu Gln Gly Val Lys Lys Lys Leu Ala 
    290                 295                 300                 


Glu His Phe Pro Asn Lys Thr Ile Lys Phe Ala Pro His Ser Gly Gly 
305                 310                 315                 320 


Asp Leu Glu Ile Thr Thr His Ser Phe Asn Cys Arg Gly Glu Phe Phe 
                325                 330                 335     


Tyr Cys Asn Thr Ser Asn Leu Phe Asn Glu Ser Asn Ile Glu Arg Asn 
            340                 345                 350         


Asp Ser Ile Ile Thr Leu Pro Cys Arg Ile Lys Gln Ile Ile Asn Met 
        355                 360                 365             


Trp Gln Glu Val Gly Arg Cys Met Tyr Ala Pro Pro Ile Ala Gly Asn 
    370                 375                 380                 


Ile Thr Cys Arg Ser Asn Ile Thr Gly Leu Leu Leu Thr Arg Asp Gly 
385                 390                 395                 400 


Gly Ser Asn Asn Asn Asp Thr Glu Thr Phe Arg Pro Gly Gly Gly Asp 
                405                 410                 415     


Met Arg Asn Asn Trp Arg Ser Glu Leu Tyr Lys Tyr Lys Val Val Glu 
            420                 425                 430         


Val Lys Pro Leu Gly Val Ala Pro Thr Glu Cys Lys Arg Arg Asn Val 
        435                 440                 445             


Thr Arg Arg Arg Arg Arg Arg Ala Val Gly Ile Gly Ala Val Phe Leu 
    450                 455                 460                 


Gly Ile Leu Gly Ala Ala Gly Ser Thr Met Gly Ala Ala Ser Asn Thr 
465                 470                 475                 480 


Leu Thr Val Gln Ala Arg Gln Leu Leu Ser Gly Ile Val Gln Gln Gln 
                485                 490                 495     


Ser Asn Leu Pro Arg Ala Pro Glu Ala Gln Gln His Met Leu Gln Leu 
            500                 505                 510         


Thr Val Trp Gly Ile Lys Gln Leu Gln Thr Arg Val Leu Ala Ile Glu 
        515                 520                 525             


Arg Tyr Leu Glu Asp Gln Gln Leu Leu Gly Leu Trp Gly Cys Ser Gly 
    530                 535                 540                 


Lys Leu Ile Cys Cys Thr Ala Val Pro Trp Asn Thr Ser Trp Ser Asn 
545                 550                 555                 560 


Lys Ser Gln Thr Asp Ile Trp Asp Asn Met Thr Trp Met Gln Trp Asp 
                565                 570                 575     


Lys Glu Ile Gly Asn Tyr Thr Gly Glu Ile Tyr Arg Leu Leu Glu Glu 
            580                 585                 590         


Ser Gln Phe Gln Gln Glu Ile 
        595                 


<210>  33
<211>  29
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  signal sequence

<400>  33

Met Arg Val Arg Gly Met Leu Arg Asn Trp Gln Gln Trp Trp Ile Trp 
1               5                   10                  15      


Ser Ser Leu Gly Phe Trp Met Leu Met Ile Tyr Ser Val 
            20                  25                  


<210>  34
<211>  29
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  signal sequence

<400>  34

Met Arg Val Met Gly Ile Gln Arg Asn Cys Gln His Leu Phe Arg Trp 
1               5                   10                  15      


Gly Thr Met Ile Leu Gly Met Ile Ile Ile Cys Ser Ala 
            20                  25                  


