                         SEQUENCE LISTING

<110>  Inivata Ltd
 
<120>  Methods for labelling nucleic acids

<130>  P106538WO

<140>  PCT/EP2017/073064
<141>  2017-09-13

<150>  GB1615486.6
<151>  2016-09-13

<160>  33    

<170>  PatentIn version 3.5

<210>  1
<211>  24
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Full-length adaptor sense strand

<400>  1
gcacctcacc tcagcatctg actc                                              24


<210>  2
<211>  25
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Full length adaptor antisense strand


<220>
<221>  misc_feature
<222>  (9)..(9)
<223>  n is I

<220>
<221>  misc_feature
<222>  (12)..(12)
<223>  n is I

<220>
<221>  misc_feature
<222>  (15)..(15)
<223>  n is I

<220>
<221>  misc_feature
<222>  (20)..(20)
<223>  n is I

<400>  2
gagtcagang cngangtgan gtgct                                             25


<210>  3
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Full length adaptor sense strand


<220>
<221>  misc_feature
<222>  (1)..(1)
<223>  n is I

<220>
<221>  misc_feature
<222>  (3)..(3)
<223>  n is I

<220>
<221>  misc_feature
<222>  (5)..(5)
<223>  n is I

<220>
<221>  misc_feature
<222>  (7)..(7)
<223>  n is I

<220>
<221>  misc_feature
<222>  (9)..(9)
<223>  n is I

<220>
<221>  misc_feature
<222>  (11)..(11)
<223>  n is I

<220>
<221>  misc_feature
<222>  (13)..(13)
<223>  n is I

<220>
<221>  misc_feature
<222>  (15)..(15)
<223>  n is I

<400>  3
ngnantncna ngnancggag t                                                 21


<210>  4
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Full length adaptor antisense strand

<400>  4
ctccgatcca tcgcaatccc                                                   20


<210>  5
<211>  33
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Assymetric Y stem adaptor sense strand

<400>  5
acactctttc cctacacgac gctcttccga tct                                    33


<210>  6
<211>  31
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Assymetric Y stem adaptor antisense strand

<400>  6
gatcggaaga gcacacgtct gaactccagt c                                      31


<210>  7
<211>  70
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  i5 PCR primer


<220>
<221>  misc_feature
<222>  (30)..(38)
<223>  Sample identifier (index sequence)

<400>  7
aatgatacgg cgaccaccga gatctacacn nnnnnnnaca ctctttccct acacgacgct       60

cttccgatct                                                              70


<210>  8
<211>  66
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  i7 PCR primer


<220>
<221>  misc_feature
<222>  (25)..(33)
<223>  Sample identifier (index sequence)

<400>  8
caagcagaag acggcatacg agatnnnnnn nngtgactgg agttcagacg tgtgctcttc       60

cgatct                                                                  66


<210>  9
<211>  24
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Full length adaptor sense strand


<220>
<221>  misc_feature
<222>  (1)..(1)
<223>  n is I

<220>
<221>  misc_feature
<222>  (5)..(5)
<223>  n is I

<220>
<221>  misc_feature
<222>  (7)..(8)
<223>  n is I

<220>
<221>  misc_feature
<222>  (10)..(10)
<223>  n is I

<220>
<221>  misc_feature
<222>  (13)..(14)
<223>  n is I

<220>
<221>  misc_feature
<222>  (16)..(16)
<223>  n is I

<220>
<221>  misc_feature
<222>  (18)..(18)
<223>  n is I

<220>
<221>  misc_feature
<222>  (21)..(21)
<223>  n is I

<400>  9
nacgngnngn cgnncnanag nagt                                              24


<210>  10
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Full length adaptor antisense strand

<400>  10
ctcctatcga acgccaaccc gtc                                               23


<210>  11
<211>  24
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Full length adaptor sense strand


<220>
<221>  misc_feature
<222>  (2)..(2)
<223>  n is I

<220>
<221>  misc_feature
<222>  (4)..(4)
<223>  n is I

<220>
<221>  misc_feature
<222>  (6)..(6)
<223>  n is I

<220>
<221>  misc_feature
<222>  (8)..(8)
<223>  n is I

<220>
<221>  misc_feature
<222>  (10)..(10)
<223>  n is I

<220>
<221>  misc_feature
<222>  (12)..(12)
<223>  n is I

<220>
<221>  misc_feature
<222>  (14)..(14)
<223>  n is I

<220>
<221>  misc_feature
<222>  (16)..(16)
<223>  n is I

<220>
<221>  misc_feature
<222>  (18)..(18)
<223>  n is I

<220>
<221>  misc_feature
<222>  (20)..(20)
<223>  n is I

<400>  11
gnangnantn gnananancn gagt                                              24


<210>  12
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Full length adaptor antisense strand

<400>  12
ctccgatcta tcccaatccc tac                                               23


<210>  13
<211>  24
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Full length adaptor sense strand


<220>
<221>  misc_feature
<222>  (4)..(4)
<223>  n is I

<220>
<221>  misc_feature
<222>  (6)..(7)
<223>  n is I

<220>
<221>  misc_feature
<222>  (9)..(10)
<223>  n is I

<220>
<221>  misc_feature
<222>  (12)..(12)
<223>  n is I

<220>
<221>  misc_feature
<222>  (14)..(14)
<223>  n is I

<220>
<221>  misc_feature
<222>  (16)..(16)
<223>  n is I

<220>
<221>  misc_feature
<222>  (18)..(18)
<223>  n is I

<220>
<221>  misc_feature
<222>  (22)..(22)
<223>  n is I

<400>  13
cgcngnngnn cnangngncg gngt                                              24


<210>  14
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Full length adaptor antisense strand

<400>  14
caccgaccca tcgcacaccc gcg                                               23


<210>  15
<211>  24
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Full length adaptor sense strand


<220>
<221>  misc_feature
<222>  (3)..(3)
<223>  n is I

<220>
<221>  misc_feature
<222>  (5)..(5)
<223>  n is I

<220>
<221>  misc_feature
<222>  (8)..(8)
<223>  n is I

<220>
<221>  misc_feature
<222>  (10)..(10)
<223>  n is I

<220>
<221>  misc_feature
<222>  (12)..(13)
<223>  n is I

<220>
<221>  misc_feature
<222>  (15)..(15)
<223>  n is I

<220>
<221>  misc_feature
<222>  (17)..(18)
<223>  n is I

<220>
<221>  misc_feature
<222>  (23)..(23)
<223>  n is I

<400>  15
aangnganan cnntngnncg gant                                              24


<210>  16
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Full length adaptor antisense strand

<400>  16
ctccgaacca ccgctatccc ctt                                               23


<210>  17
<211>  25
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Full length adaptor sense strand

<400>  17
gcacctcacc tcagcatcga ctgag                                             25


<210>  18
<211>  26
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Full length adaptor antisense strand


<220>
<221>  misc_feature
<222>  (10)..(10)
<223>  n is I

<220>
<221>  misc_feature
<222>  (13)..(13)
<223>  n is I

<220>
<221>  misc_feature
<222>  (16)..(16)
<223>  n is I

<220>
<221>  misc_feature
<222>  (21)..(21)
<223>  n is I

<400>  18
ctcagtcgan gcngangtga ngtgct                                            26


<210>  19
<211>  26
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Full length adaptor sense strand

<400>  19
gcacctcacc tcagcatcta cagtct                                            26


<210>  20
<211>  27
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Full length adaptor antisense strand


<220>
<221>  misc_feature
<222>  (11)..(11)
<223>  n is I

<220>
<221>  misc_feature
<222>  (14)..(14)
<223>  n is I

<220>
<221>  misc_feature
<222>  (17)..(17)
<223>  n is I

<220>
<221>  misc_feature
<222>  (22)..(22)
<223>  n is I

<400>  20
agactgtaga ngcngangtg angtgct                                           27


<210>  21
<211>  27
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Full length adaptor sense strand

<400>  21
gcacctcacc tcagcatcaa tgtcaga                                           27


<210>  22
<211>  28
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Full length adaptor antisense strand


<220>
<221>  misc_feature
<222>  (12)..(12)
<223>  n is I

<220>
<221>  misc_feature
<222>  (15)..(15)
<223>  n is I

<220>
<221>  misc_feature
<222>  (18)..(18)
<223>  n is I

<220>
<221>  misc_feature
<222>  (23)..(23)
<223>  n is I

<400>  22
tctgacattg angcngangt gangtgct                                          28


<210>  23
<211>  41
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Exstension adaptor and NAOI molecule sequences

<400>  23
tgcgtagcgg tgcaagcagc cgtacatgag accgctacgc a                           41


<210>  24
<211>  41
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Extension adaptor and NAOI sequence

<400>  24
tgcgtagcgg tgcaagcagc cgtacatgag accgctacgc a                           41


<210>  25
<211>  41
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Extension adaptor and NAOI sequence

<400>  25
tgaatgatcg tgcatgcagc cgtacatgag accgctacgc a                           41


<210>  26
<211>  41
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Extension adaptor and NAOI sequence

<400>  26
tgccgatagg tgcatgcagc cgtacatgag accgctacgc a                           41


<210>  27
<211>  41
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Extension adaptor and NAOI sequence

<400>  27
tgcgtagcgg tgcaagcagc cgtacatgag accgctacgc a                           41


<210>  28
<211>  40
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Extension adaptor and NAOI sequence

<400>  28
tggtagcatg tgcagaagcc gtagatgaga cttgacgaca                             40


<210>  29
<211>  41
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Extension adaptor and NAOI sequence

<400>  29
tgtgatcatg tgcatgcagc cgtagatgag acttgacgac a                           41


<210>  30
<211>  41
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Extension adaptor and NAOI sequence

<400>  30
tgttagctag tgcatgcagc cgtagatgag acttgacgac a                           41


<210>  31
<211>  41
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Extension adaptor and NAOI sequence

<400>  31
tgcgtatcgg tgcatgcagc cgtagatgag acttgacgac a                           41


<210>  32
<211>  41
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Extension adaptor and NAOI sequence

<400>  32
tgagtgatcg tgcatgcagc cgtagatgag acttgacgac a                           41


<210>  33
<211>  41
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Extension adaptor and NAOI sequence

<400>  33
tgcttagcgg tgcatgcagc cgtagatgag acttgacgac a                           41


