                         SEQUENCE LISTING

<110>  Intrexon Actobiotics NV
 
<120>  Genetically Modified Bacteria

<130>  205350-0032-00-WO-567020

<150>  US62/383,079
<151>  2016-09-02

<160>  24    

<170>  PatentIn version 3.5

<210>  1
<211>  160
<212>  PRT
<213>  Homo sapiens


<220>
<221>  MISC
<222>  (1)..(160)
<223>  human IL-10 sequence without signal peptide

<400>  1

Ser Ala Gly Gln Gly Thr Gln Ser Glu Asn Ser Cys Thr His Phe Pro 
1               5                   10                  15      


Gly Asn Leu Pro Asn Met Leu Arg Asp Leu Arg Asp Ala Phe Ser Arg 
            20                  25                  30          


Val Lys Thr Phe Phe Gln Met Lys Asp Gln Leu Asp Asn Leu Leu Leu 
        35                  40                  45              


Lys Glu Ser Leu Leu Glu Asp Phe Lys Gly Tyr Leu Gly Cys Gln Ala 
    50                  55                  60                  


Leu Ser Glu Met Ile Gln Phe Tyr Leu Glu Glu Val Met Pro Gln Ala 
65                  70                  75                  80  


Glu Asn Gln Asp Pro Asp Ile Lys Ala His Val Asn Ser Leu Gly Glu 
                85                  90                  95      


Asn Leu Lys Thr Leu Arg Leu Arg Leu Arg Arg Cys His Arg Phe Leu 
            100                 105                 110         


Pro Cys Glu Asn Lys Ser Lys Ala Val Glu Gln Val Lys Asn Ala Phe 
        115                 120                 125             


Asn Lys Leu Gln Glu Lys Gly Ile Tyr Lys Ala Met Ser Glu Phe Asp 
    130                 135                 140                 


Ile Phe Ile Asn Tyr Ile Glu Ala Tyr Met Thr Met Lys Ile Arg Asn 
145                 150                 155                 160 


<210>  2
<211>  480
<212>  DNA
<213>  Homo sapiens


<220>
<221>  MISC
<222>  (1)..(480)
<223>  human IL-10 coding sequence without signal peptide

<400>  2
tcagctggtc aaggtactca atcagaaaac tcatgtactc actttccagg taacttgcca       60

aacatgcttc gtgatttgcg tgatgctttt tcacgtgtta aaactttttt tcaaatgaaa      120

gatcaacttg ataacttgct tttgaaagaa tcacttttgg aagattttaa aggttacctt      180

ggttgtcaag ctttgtcaga aatgatccaa ttttaccttg aagaagttat gccacaagct      240

gaaaaccaag atccagatat caaagctcac gttaactcat tgggtgaaaa ccttaaaact      300

ttgcgtcttc gtttgcgtcg ttgtcaccgt tttcttccat gtgaaaacaa atcaaaagct      360

gttgaacaag ttaaaaacgc ttttaacaaa ttgcaagaaa aaggtatcta caaagctatg      420

tcagaatttg atatctttat caactacatc gaagcttaca tgactatgaa aatccgtaac      480


<210>  3
<211>  86
<212>  PRT
<213>  Homo sapiens


<220>
<221>  MISC
<222>  (1)..(86)
<223>  human proinsulin (PINS) without signal peptide

<400>  3

Phe Val Asn Gln His Leu Cys Gly Ser His Leu Val Glu Ala Leu Tyr 
1               5                   10                  15      


Leu Val Cys Gly Glu Arg Gly Phe Phe Tyr Thr Pro Lys Thr Arg Arg 
            20                  25                  30          


Glu Ala Glu Asp Leu Gln Val Gly Gln Val Glu Leu Gly Gly Gly Pro 
        35                  40                  45              


Gly Ala Gly Ser Leu Gln Pro Leu Ala Leu Glu Gly Ser Leu Gln Lys 
    50                  55                  60                  


Arg Gly Ile Val Glu Gln Cys Cys Thr Ser Ile Cys Ser Leu Tyr Gln 
65                  70                  75                  80  


Leu Glu Asn Tyr Cys Asn 
                85      


<210>  4
<211>  258
<212>  DNA
<213>  Homo sapiens


<220>
<221>  MISC
<222>  (1)..(258)
<223>  human proinsulin (PINS) coding sequence without signal peptide

<400>  4
tttgtgaacc aacacctgtg cggctcacac ctggtggaag ctctctacct agtgtgcggg       60

gaacgaggct tcttctacac acccaagacc cgccgggagg cagaggacct gcaggtgggg      120

caggtggagc tgggcggggg ccctggtgca ggcagcctgc agcccttggc cctggagggg      180

tccctgcaga agcgtggcat tgtggaacaa tgctgtacca gcatctgctc cctctaccag      240

ctggagaact actgcaac                                                    258


<210>  5
<211>  110
<212>  PRT
<213>  Homo sapiens


<220>
<221>  MISC
<222>  (1)..(110)
<223>  wild-type human proinsulin (PINS) sequence

<400>  5

Met Ala Leu Trp Met Arg Leu Leu Pro Leu Leu Ala Leu Leu Ala Leu 
1               5                   10                  15      


Trp Gly Pro Asp Pro Ala Ala Ala Phe Val Asn Gln His Leu Cys Gly 
            20                  25                  30          


Ser His Leu Val Glu Ala Leu Tyr Leu Val Cys Gly Glu Arg Gly Phe 
        35                  40                  45              


Phe Tyr Thr Pro Lys Thr Arg Arg Glu Ala Glu Asp Leu Gln Val Gly 
    50                  55                  60                  


Gln Val Glu Leu Gly Gly Gly Pro Gly Ala Gly Ser Leu Gln Pro Leu 
65                  70                  75                  80  


Ala Leu Glu Gly Ser Leu Gln Lys Arg Gly Ile Val Glu Gln Cys Cys 
                85                  90                  95      


Thr Ser Ile Cys Ser Leu Tyr Gln Leu Glu Asn Tyr Cys Asn 
            100                 105                 110 


<210>  6
<211>  333
<212>  DNA
<213>  Homo sapiens


<220>
<221>  MISC
<222>  (1)..(333)
<223>  wild-type human PINS open reading frame

<400>  6
atggccctgt ggatgcgcct cctgcccctg ctggcgctgc tggccctctg gggacctgac       60

ccagccgcag cctttgtgaa ccaacacctg tgcggctcac acctggtgga agctctctac      120

ctagtgtgcg gggaacgagg cttcttctac acacccaaga cccgccggga ggcagaggac      180

ctgcaggtgg ggcaggtgga gctgggcggg ggccctggtg caggcagcct gcagcccttg      240

gccctggagg ggtccctgca gaagcgtggc attgtggaac aatgctgtac cagcatctgc      300

tccctctacc agctggagaa ctactgcaac tag                                   333


<210>  7
<211>  300
<212>  DNA
<213>  Homo sapiens


<220>
<221>  MISC
<222>  (1)..(300)
<223>  human PINS splice variant; AY899304; coding sequence is nt 56-388

<400>  7
gccatcaagc aggtctgttc caagggcctt tgcgtcagat cactgtcctt ctgccatggc       60

cctgtggatg cgcctcctgc ccctgctggc gctgctggcc ctctggggac ctgacccagc      120

cgcagccttt gtgaaccaac acctgtgcgg ctcacacctg gtggaagctc tctacctagt      180

gtgcggggaa cgaggcttct tctacacacc caagacccgc cgggaggcag aggacctgca      240

ggtggggcag gtggagctgg gcgggggccc tggtgcaggc agcctgcagc ccttggccct      300


<210>  8
<211>  16
<212>  DNA
<213>  Homo sapiens


<220>
<221>  MISC
<222>  (1)..(16)
<223>  5' intergenic region of rpmD gene; nt 1-3 is the stop codon of 
       preceding gene and nt 14-16 is the ATG of the subsequent gene

<400>  8
taaggaggaa aaaatg                                                       16


<210>  9
<211>  10
<212>  DNA
<213>  Homo sapiens


<220>
<221>  MISC
<222>  (1)..(10)
<223>  5' intergenic region rpmD gene without stop and start codons

<400>  9
ggaggaaaaa                                                              10


<210>  10
<211>  27
<212>  PRT
<213>  Lactococcus lactis


<220>
<221>  MISC
<222>  (1)..(27)
<223>  Secretion leader SSusp45

<400>  10

Met Lys Lys Lys Ile Ile Ser Ala Ile Leu Met Ser Thr Val Ile Leu 
1               5                   10                  15      


Ser Ala Ala Ala Pro Leu Ser Gly Val Tyr Ala 
            20                  25          


<210>  11
<211>  81
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Chemically synthesized


<220>
<221>  MISC
<222>  (1)..(81)
<223>  Encodes SSusp45 secretion leader sequence

<400>  11
atgaaaaaaa agattatctc agctatttta atgtctacag tgatactttc tgctgcagcc       60

ccgttgtcag gtgtttacgc c                                                 81


<210>  12
<211>  81
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Chemically synthesized


<220>
<221>  MISC
<222>  (1)..(81)
<223>  Encodes SSusp45 secretion leader sequence

<400>  12
atgaagaaga aaatcattag tgccatctta atgtctacag tgattctttc agctgcagct       60

cctttatcag gcgtttatgc a                                                 81


<210>  13
<211>  720
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  genetically engineered construct


<220>
<221>  MISC
<222>  (1)..(720)
<223>  IL-10 expression cassette

<220>
<221>  CDS
<222>  (105)..(665)
<223>  nt 105-185 encodes SSusp45 secretion leader; nt 185-665 encodes 
       hIL10aPxA; stop codon is nt 666-668

<400>  13
aaaacgcctt aaaatggcat tttgacttgc aaactgggct aagatttgct aaaatgaaaa       60

atgcctatgt ttaaggtaaa aaacaaatgg aggacatttc taaa atg aaa aaa aag       116
                                                 Met Lys Lys Lys          
                                                 1                        

att atc tca gct att tta atg tct aca gtg ata ctt tct gct gca gcc        164
Ile Ile Ser Ala Ile Leu Met Ser Thr Val Ile Leu Ser Ala Ala Ala           
5                   10                  15                  20            

ccg ttg tca ggt gtt tac gcc tca gct ggt caa ggt act caa tca gaa        212
Pro Leu Ser Gly Val Tyr Ala Ser Ala Gly Gln Gly Thr Gln Ser Glu           
                25                  30                  35                

aac tca tgt act cac ttt cca ggt aac ttg cca aac atg ctt cgt gat        260
Asn Ser Cys Thr His Phe Pro Gly Asn Leu Pro Asn Met Leu Arg Asp           
            40                  45                  50                    

ttg cgt gat gct ttt tca cgt gtt aaa act ttt ttt caa atg aaa gat        308
Leu Arg Asp Ala Phe Ser Arg Val Lys Thr Phe Phe Gln Met Lys Asp           
        55                  60                  65                        

caa ctt gat aac ttg ctt ttg aaa gaa tca ctt ttg gaa gat ttt aaa        356
Gln Leu Asp Asn Leu Leu Leu Lys Glu Ser Leu Leu Glu Asp Phe Lys           
    70                  75                  80                            

ggt tac ctt ggt tgt caa gct ttg tca gaa atg atc caa ttt tac ctt        404
Gly Tyr Leu Gly Cys Gln Ala Leu Ser Glu Met Ile Gln Phe Tyr Leu           
85                  90                  95                  100           

gaa gaa gtt atg cca caa gct gaa aac caa gat cca gat atc aaa gct        452
Glu Glu Val Met Pro Gln Ala Glu Asn Gln Asp Pro Asp Ile Lys Ala           
                105                 110                 115               

cac gtt aac tca ttg ggt gaa aac ctt aaa act ttg cgt ctt cgt ttg        500
His Val Asn Ser Leu Gly Glu Asn Leu Lys Thr Leu Arg Leu Arg Leu           
            120                 125                 130                   

cgt cgt tgt cac cgt ttt ctt cca tgt gaa aac aaa tca aaa gct gtt        548
Arg Arg Cys His Arg Phe Leu Pro Cys Glu Asn Lys Ser Lys Ala Val           
        135                 140                 145                       

gaa caa gtt aaa aac gct ttt aac aaa ttg caa gaa aaa ggt atc tac        596
Glu Gln Val Lys Asn Ala Phe Asn Lys Leu Gln Glu Lys Gly Ile Tyr           
    150                 155                 160                           

aaa gct atg tca gaa ttt gat atc ttt atc aac tac atc gaa gct tac        644
Lys Ala Met Ser Glu Phe Asp Ile Phe Ile Asn Tyr Ile Glu Ala Tyr           
165                 170                 175                 180           

atg act atg aaa atc cgt aac taactagaat taatctataa gttactgaca           695
Met Thr Met Lys Ile Arg Asn                                               
                185                                                       

aaactgtcag taactttttt tgtgg                                            720


<210>  14
<211>  187
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic Construct

<400>  14

Met Lys Lys Lys Ile Ile Ser Ala Ile Leu Met Ser Thr Val Ile Leu 
1               5                   10                  15      


Ser Ala Ala Ala Pro Leu Ser Gly Val Tyr Ala Ser Ala Gly Gln Gly 
            20                  25                  30          


Thr Gln Ser Glu Asn Ser Cys Thr His Phe Pro Gly Asn Leu Pro Asn 
        35                  40                  45              


Met Leu Arg Asp Leu Arg Asp Ala Phe Ser Arg Val Lys Thr Phe Phe 
    50                  55                  60                  


Gln Met Lys Asp Gln Leu Asp Asn Leu Leu Leu Lys Glu Ser Leu Leu 
65                  70                  75                  80  


Glu Asp Phe Lys Gly Tyr Leu Gly Cys Gln Ala Leu Ser Glu Met Ile 
                85                  90                  95      


Gln Phe Tyr Leu Glu Glu Val Met Pro Gln Ala Glu Asn Gln Asp Pro 
            100                 105                 110         


Asp Ile Lys Ala His Val Asn Ser Leu Gly Glu Asn Leu Lys Thr Leu 
        115                 120                 125             


Arg Leu Arg Leu Arg Arg Cys His Arg Phe Leu Pro Cys Glu Asn Lys 
    130                 135                 140                 


Ser Lys Ala Val Glu Gln Val Lys Asn Ala Phe Asn Lys Leu Gln Glu 
145                 150                 155                 160 


Lys Gly Ile Tyr Lys Ala Met Ser Glu Phe Asp Ile Phe Ile Asn Tyr 
                165                 170                 175     


Ile Glu Ala Tyr Met Thr Met Lys Ile Arg Asn 
            180                 185         


<210>  15
<211>  1680
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  genetically engineered construct


<220>
<221>  MISC
<222>  (1)..(1680)
<223>  PINS polycistronic expression cassette

<220>
<221>  CDS
<222>  (277)..(1284)
<223>  gapB gene coding sequence

<220>
<221>  misc_feature
<222>  (1285)..(1300)
<223>  intergenic region (rpmD); 1285-1287 is stop codon of preceding 
       coding sequence and 1298-1300 is the ATG of the subsequent coding
       sequence

<220>
<221>  CDS
<222>  (1298)..(1636)
<223>  Secretion sequence SSusp45 encoded by nt. 1298-1378; Human PINS 
       encoded by 1379-1636

<400>  15
aataaaaatt actgacagcc tgctcagtaa tttttttagt cataattttt aggtggaaag       60

tcaaagatta ttgccaaaag tattagcttt tttaatgtta accgctttca gaagaagggg      120

agttcatttg cttttgtaga gcgctttcta aggtagttta tgtttgcaaa ttttaaaaaa      180

agtgttaaaa taaaagagta agttaaattg ttaacttagt caatttaaaa ggtttgcctt      240

ttataaaatc taatccctat aaggaggaaa ctacta atg gta gtt aaa gtt ggt        294
                                        Met Val Val Lys Val Gly           
                                        1               5                 

att aac ggt ttc ggt cgt atc ggt cgt ctt gct ttc cgt cgt att caa        342
Ile Asn Gly Phe Gly Arg Ile Gly Arg Leu Ala Phe Arg Arg Ile Gln           
            10                  15                  20                    

aat gtt gaa ggt gtt gaa gtt gtt gca atc aac gac ttg aca gat cca        390
Asn Val Glu Gly Val Glu Val Val Ala Ile Asn Asp Leu Thr Asp Pro           
        25                  30                  35                        

gca atg ctt gct cac ttg ctt aaa tac gat aca act caa ggt cgt ttt        438
Ala Met Leu Ala His Leu Leu Lys Tyr Asp Thr Thr Gln Gly Arg Phe           
    40                  45                  50                            

gat ggt aaa gtt gaa gtt aaa gat ggt ggt ttt gaa gtt aac ggt aaa        486
Asp Gly Lys Val Glu Val Lys Asp Gly Gly Phe Glu Val Asn Gly Lys           
55                  60                  65                  70            

ttc gtt aaa gtt act gct gaa tct aac cca gct aac atc aac tgg gct        534
Phe Val Lys Val Thr Ala Glu Ser Asn Pro Ala Asn Ile Asn Trp Ala           
                75                  80                  85                

gaa gtt ggt gca gaa atc gtt ctt gaa gca act ggt ttc ttc gca act        582
Glu Val Gly Ala Glu Ile Val Leu Glu Ala Thr Gly Phe Phe Ala Thr           
            90                  95                  100                   

aaa gaa aaa gct gaa caa cac ttg cac gct aat ggt gct aag aaa gtt        630
Lys Glu Lys Ala Glu Gln His Leu His Ala Asn Gly Ala Lys Lys Val           
        105                 110                 115                       

gtt atc act gca cct ggt gga tca gat gtt aaa aca atc gtt ttc aac        678
Val Ile Thr Ala Pro Gly Gly Ser Asp Val Lys Thr Ile Val Phe Asn           
    120                 125                 130                           

act aac cac gaa gta ctt gat gga act gaa aca gta att tca gct ggt        726
Thr Asn His Glu Val Leu Asp Gly Thr Glu Thr Val Ile Ser Ala Gly           
135                 140                 145                 150           

tca tgt aca acc aac tgt ctt gct cca atg gct gat act ttg aac aaa        774
Ser Cys Thr Thr Asn Cys Leu Ala Pro Met Ala Asp Thr Leu Asn Lys           
                155                 160                 165               

caa ttc ggt atc aaa gtt ggt aca atg act aca gtt cac ggt tac act        822
Gln Phe Gly Ile Lys Val Gly Thr Met Thr Thr Val His Gly Tyr Thr           
            170                 175                 180                   

ggt gac caa atg act ctt gat ggc cca cac cgt ggt gga gat ttc cgt        870
Gly Asp Gln Met Thr Leu Asp Gly Pro His Arg Gly Gly Asp Phe Arg           
        185                 190                 195                       

cgc gca cgt gct gca gct gaa aac atc gta cct aac tca aca ggt gct        918
Arg Ala Arg Ala Ala Ala Glu Asn Ile Val Pro Asn Ser Thr Gly Ala           
    200                 205                 210                           

gct aaa gcc atc ggt ctt gta ttg cca gaa ctt caa ggt aaa ctt caa        966
Ala Lys Ala Ile Gly Leu Val Leu Pro Glu Leu Gln Gly Lys Leu Gln           
215                 220                 225                 230           

gga cat gct caa cgt gta cca gtt cca act ggt tca ttg act gaa ctt       1014
Gly His Ala Gln Arg Val Pro Val Pro Thr Gly Ser Leu Thr Glu Leu           
                235                 240                 245               

gtt act atc ctt gat aaa gaa gtt aca gtt gac gaa atc aac gca gct       1062
Val Thr Ile Leu Asp Lys Glu Val Thr Val Asp Glu Ile Asn Ala Ala           
            250                 255                 260                   

atg aaa gct gct tca aat gaa tca ttt ggt tac aac gaa gac caa atc       1110
Met Lys Ala Ala Ser Asn Glu Ser Phe Gly Tyr Asn Glu Asp Gln Ile           
        265                 270                 275                       

gtt tca tct gat atc gtt ggt atc tca aac tct tca ctc ttt gat gct       1158
Val Ser Ser Asp Ile Val Gly Ile Ser Asn Ser Ser Leu Phe Asp Ala           
    280                 285                 290                           

act caa act gaa gtt act tca gct aac gga gct caa ctt gtt aaa act       1206
Thr Gln Thr Glu Val Thr Ser Ala Asn Gly Ala Gln Leu Val Lys Thr           
295                 300                 305                 310           

gta tct tgg tac gat aac gaa atg tca tac act tca aac ctt gtt cgt       1254
Val Ser Trp Tyr Asp Asn Glu Met Ser Tyr Thr Ser Asn Leu Val Arg           
                315                 320                 325               

aca ctt gca tac ttc gct aaa atc gct aaa taaggaggaa aaa atg aag        1303
Thr Leu Ala Tyr Phe Ala Lys Ile Ala Lys                Met Lys            
            330                 335                                       

aag aaa atc att agt gcc atc tta atg tct aca gtg att ctt tca gct       1351
Lys Lys Ile Ile Ser Ala Ile Leu Met Ser Thr Val Ile Leu Ser Ala           
    340                 345                 350                           

gca gct cct tta tca ggc gtt tat gca ttt gtg aac caa cac ctg tgc       1399
Ala Ala Pro Leu Ser Gly Val Tyr Ala Phe Val Asn Gln His Leu Cys           
355                 360                 365                 370           

ggc tca cac ctg gtg gaa gct ctc tac cta gtg tgc ggg gaa cga ggc       1447
Gly Ser His Leu Val Glu Ala Leu Tyr Leu Val Cys Gly Glu Arg Gly           
                375                 380                 385               

ttc ttc tac aca ccc aag acc cgc cgg gag gca gag gac ctg cag gtg       1495
Phe Phe Tyr Thr Pro Lys Thr Arg Arg Glu Ala Glu Asp Leu Gln Val           
            390                 395                 400                   

ggg cag gtg gag ctg ggc ggg ggc cct ggt gca ggc agc ctg cag ccc       1543
Gly Gln Val Glu Leu Gly Gly Gly Pro Gly Ala Gly Ser Leu Gln Pro           
        405                 410                 415                       

ttg gcc ctg gag ggg tcc ctg cag aag cgt ggc att gtg gaa caa tgc       1591
Leu Ala Leu Glu Gly Ser Leu Gln Lys Arg Gly Ile Val Glu Gln Cys           
    420                 425                 430                           

tgt acc agc atc tgc tcc ctc tac cag ctg gag aac tac tgc aac           1636
Cys Thr Ser Ile Cys Ser Leu Tyr Gln Leu Glu Asn Tyr Cys Asn               
435                 440                 445                               

taattttccg attttaacgg tataaaaacc agtcttcggg ctgg                      1680


<210>  16
<211>  336
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic Construct

<400>  16

Met Val Val Lys Val Gly Ile Asn Gly Phe Gly Arg Ile Gly Arg Leu 
1               5                   10                  15      


Ala Phe Arg Arg Ile Gln Asn Val Glu Gly Val Glu Val Val Ala Ile 
            20                  25                  30          


Asn Asp Leu Thr Asp Pro Ala Met Leu Ala His Leu Leu Lys Tyr Asp 
        35                  40                  45              


Thr Thr Gln Gly Arg Phe Asp Gly Lys Val Glu Val Lys Asp Gly Gly 
    50                  55                  60                  


Phe Glu Val Asn Gly Lys Phe Val Lys Val Thr Ala Glu Ser Asn Pro 
65                  70                  75                  80  


Ala Asn Ile Asn Trp Ala Glu Val Gly Ala Glu Ile Val Leu Glu Ala 
                85                  90                  95      


Thr Gly Phe Phe Ala Thr Lys Glu Lys Ala Glu Gln His Leu His Ala 
            100                 105                 110         


Asn Gly Ala Lys Lys Val Val Ile Thr Ala Pro Gly Gly Ser Asp Val 
        115                 120                 125             


Lys Thr Ile Val Phe Asn Thr Asn His Glu Val Leu Asp Gly Thr Glu 
    130                 135                 140                 


Thr Val Ile Ser Ala Gly Ser Cys Thr Thr Asn Cys Leu Ala Pro Met 
145                 150                 155                 160 


Ala Asp Thr Leu Asn Lys Gln Phe Gly Ile Lys Val Gly Thr Met Thr 
                165                 170                 175     


Thr Val His Gly Tyr Thr Gly Asp Gln Met Thr Leu Asp Gly Pro His 
            180                 185                 190         


Arg Gly Gly Asp Phe Arg Arg Ala Arg Ala Ala Ala Glu Asn Ile Val 
        195                 200                 205             


Pro Asn Ser Thr Gly Ala Ala Lys Ala Ile Gly Leu Val Leu Pro Glu 
    210                 215                 220                 


Leu Gln Gly Lys Leu Gln Gly His Ala Gln Arg Val Pro Val Pro Thr 
225                 230                 235                 240 


Gly Ser Leu Thr Glu Leu Val Thr Ile Leu Asp Lys Glu Val Thr Val 
                245                 250                 255     


Asp Glu Ile Asn Ala Ala Met Lys Ala Ala Ser Asn Glu Ser Phe Gly 
            260                 265                 270         


Tyr Asn Glu Asp Gln Ile Val Ser Ser Asp Ile Val Gly Ile Ser Asn 
        275                 280                 285             


Ser Ser Leu Phe Asp Ala Thr Gln Thr Glu Val Thr Ser Ala Asn Gly 
    290                 295                 300                 


Ala Gln Leu Val Lys Thr Val Ser Trp Tyr Asp Asn Glu Met Ser Tyr 
305                 310                 315                 320 


Thr Ser Asn Leu Val Arg Thr Leu Ala Tyr Phe Ala Lys Ile Ala Lys 
                325                 330                 335     


<210>  17
<211>  113
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic Construct

<400>  17

Met Lys Lys Lys Ile Ile Ser Ala Ile Leu Met Ser Thr Val Ile Leu 
1               5                   10                  15      


Ser Ala Ala Ala Pro Leu Ser Gly Val Tyr Ala Phe Val Asn Gln His 
            20                  25                  30          


Leu Cys Gly Ser His Leu Val Glu Ala Leu Tyr Leu Val Cys Gly Glu 
        35                  40                  45              


Arg Gly Phe Phe Tyr Thr Pro Lys Thr Arg Arg Glu Ala Glu Asp Leu 
    50                  55                  60                  


Gln Val Gly Gln Val Glu Leu Gly Gly Gly Pro Gly Ala Gly Ser Leu 
65                  70                  75                  80  


Gln Pro Leu Ala Leu Glu Gly Ser Leu Gln Lys Arg Gly Ile Val Glu 
                85                  90                  95      


Gln Cys Cys Thr Ser Ile Cys Ser Leu Tyr Gln Leu Glu Asn Tyr Cys 
            100                 105                 110         


Asn 
    


<210>  18
<211>  3289
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Genetically engineered construct

<400>  18
gatggctgaa gctccaactc atgaacaagt tgaccatgtt gtggatacaa ttgttgaagt       60

tgttgaagag gaaattggtg tgaaataaag aaaagacaag gagaatattc ttcttgtctt      120

ttttcatatc ctaaaactct acctactgtg gtagagtttt tttatctttt ttggcgtcta      180

gcaaactctg taaaacgaaa acggtcaacc tgatgtcgtg attcagtata ctggaaaagt      240

gtattatcgg caagatagac atgagatttc acggaaacaa catgatggtc cttcggattt      300

aaatcaagat atgtaaaatc atcttcacaa gcaaaatcaa tggtaacttc tttttgggca      360

taggcaatat caagtcccaa agccccttct aaataatcgt aagtagaatt ttgggcatgt      420

gcgggggtca aaccatcagc gtatttttct aaaaataaat cccaatccaa aatggaaaat      480

ttaccatcta cttttcttct tctaagaata ctaagggctt ggtcgccgat ggcgaatcca      540

gtagtttctg aaagagctgg ggtaattttt atactttcaa attttattac ttcagtttca      600

ctgtgaaaac ccattgaagt ttgcaattct ttatatgaag ttaagccgga aatagggaaa      660

aggagccgat cgtgagcgag gacaatgctg ccatagccat gtcttctttg gatgagccct      720

ttttcttcta aaatttttaa agcttgtctg acggttgaac ggctactttc ataactaata      780

gaaagttcat tctcgcttgg aagaatatcg ttcgttttat agatatcatt aaaaatcttt      840

ttttctaaat cttgcaaaat cacttcatat ttcttcatac tttatatttt atcataaaaa      900

taattgttaa cgcttgctga aaacgttttt atgaaaacgc cttaaaatgg cattttgact      960

tgcaaactgg gctaagattt gctaaaatga aaaatgccta tgtttaaggt aaaaaacaaa     1020

tggaggacat ttctaaaatg tttggaatag gaaaaaagaa agaattgaga gatgataaaa     1080

gcctttatgc tccagtttct ggggaagtta tcaacctttc aacagtcaac gaccccgtat     1140

tttcaaaaaa gataatggga gacgggttcg cggttgagcc aaaagaaaat aaaatttttg     1200

ccccagtttc tgcaaaagta actttggttc aaggacatgc aattggtttt aaacgtgctg     1260

atggcttaga tgtactttta catcttggaa ttgatacagt agctcttaaa ggtcttcatt     1320

ttaaaatcaa ggtcaaagtt gatgatattg tcaatggtgg tgatgagctt ggaagcgttg     1380

attgggcaca gattgaagct gcaggtttag ataaaacgac aatggttatc tttacaaata     1440

caaaagataa actctctgag ttcaatgtca attatggacc agctacttct ggaagtgaac     1500

ttggtaaggc aagtgttaaa taaggaggaa aaaatggcaa attattcaca acttgcgaca     1560

gaaattatcg caaatgtagg tggcgctgag aatgtcacaa aagttattca ctgtatcact     1620

cgtcttcgtt ttaccttgaa agacaaagat aaagcagata cggcggcgat tgaagcctta     1680

cctggtgtcg ctggagctgt ttataactca aacttgaatc aatatcaagt agttattgga     1740

caagctgtag aagatgttta tgacgaggtt gttgaacagc ttggagattc agttgttgat     1800

gaagatgcaa cggcgcaagc acttactgca acagcaccgg ctagtggtaa aaaacaaaat     1860

ccaattgttc atgctttcca agtggttatt gggacaatta caggttcgat gattccaatt     1920

attggtttac ttgcggctgg tgggatgatt aatggattat taagtatctt tgttaaagga     1980

aatcgtttaa ttgaagtgat tgaccctgca agttcaactt acgtcattat ctcaactcta     2040

gcaatgacac cattttattt cttacctgtt ttagtaggat tttcagcagc aaaacaatta     2100

gcacctaaag atactgtttt acaatttatt ggtgctgctg ttggtggttt catgattaat     2160

ccagggatta ctaacttggt aaatgctcat gttggaacaa atgcggccgg taaaaatgtt     2220

gttgttgaag cagcagctcc agtagcaaat ttccttggag tcacttttaa tacaagttat     2280

tttggaattc cggttgcttt gccaagttat gcttatacaa ttttcccaat cattgtggcg     2340

gtagcaatcg ctaaaccttt gaatgcttgg ttgaaaaagg ttttaccact tgccttgcgt     2400

ccaattttcc aaccgatgat tactttcttc atcactgctt caatcatttt actcttggtc     2460

ggtcctgtta tttcaacaat ttcatctggt ttgtcattcg ttattgacca tatcttgtca     2520

ttaaacttag ggattgcaag tattatcgtc ggtggtttgt atcaatgttt ggttatattt     2580

ggtttgcact ggttggttgt accacttatt tcacaagagt tggcagcaac aggagcaagc     2640

tcacttaata tgattgttag cttcacaatg cttgcgcaag gagttggtgc cttgactgtc     2700

ttctttaaat ctaaaaaagc tgaccttaaa ggactttctg ctccagctgc catttcggct     2760

ttttgtggag taactgaacc tgccatgtac ggaattaact tgaaatatgt tcgcgtcttc     2820

atcatgtctt caattggtgc agcaattggt gctgggattg ccggatttgg tggcttacaa     2880

atgtttggat tttcagggtc attgattagt tttcctaact ttatctctaa tccattgacg     2940

catcatgcac ctgcgggtaa cttaatgctc ttctggattg ccactgcggt atgtgctgtt     3000

gccactttct tattagtttg gttctttggt tacaaggata ctgatgtcat gggacaagga     3060

gttgaacaaa aaaatgcatt taaggatgct gtaaaataaa tagttttgct cttaataaag     3120

ttttgataca aggatttaca attatttttt gataaaaaaa ttactgatag aaatgaaaaa     3180

aattctgtca gtaattttgg aaagtcattc taaaaaattc attttaaaat gacgagaaag     3240

aaggtaaaaa gatgtttaaa gcagtattgt ttgatttaga tggcgtaat                 3289


<210>  19
<211>  1700
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Genetically engineered construct

<400>  19
gtaattctaa tgctggtggg aatacaaatt caggcactag tactggaaat actggaggaa       60

caactactgg tggtagcggt ataaatagtt caccaattgg aaatccttat gctggtggtg      120

gatgtactga ctatgtatgg caatactttg ctgcacaagg aatttatatc agaaatatca      180

tgcctggtaa tggtggacaa tgggcttcta atggacctgc ccaaggcgtg ctccatgttg      240

taggagctgc tcctggtgtt atcgcatcaa gcttctcagc tgattttgtt ggatatgcaa      300

actcacctta cggtcacgta gctattgtaa aatcagttaa ttcagatggt acaattacta      360

tcaaagaagg cggatatggt acaacttggt ggggacatga acgtactgta agtgcgtctg      420

gtgttacttt cttgatgcca aactaaggag gaaaaaatga cagaaccgtt aaccgaaacc      480

cctgaactat ccgcgaaata tgcctggttt tttgatcttg atggaacgct ggcggaaatc      540

aaaccgcatc ccgatcaggt cgtcgtgcct gacaatattc tgcaaggact acagctactg      600

gcaaccgcaa gtgatggtgc attggcattg atatcagggc gctcaatggt ggagcttgac      660

gcactggcaa aaccttatcg cttcccgtta gcgggcgtgc atggggcgga gcgccgtgac      720

atcaatggta aaacacatat cgttcatctg ccggatgcga ttgcgcgtga tattagcgtg      780

caactgcata cagtcatcgc tcagtatccc ggcgcggagc tggaggcgaa agggatggct      840

tttgcgctgc attatcgtca ggctccgcag catgaagacg cattaatgac attagcgcaa      900

cgtattactc agatctggcc acaaatggcg ttacagcagg gaaagtgtgt tgtcgagatc      960

aaaccgagag gtaccagtaa aggtgaggca attgcagctt ttatgcagga agctcccttt     1020

atcgggcgaa cgcccgtatt tctgggcgat gatttaaccg atgaatctgg cttcgcagtc     1080

gttaaccgac tgggcggaat gtcagtaaaa attggcacag gtgcaactca ggcatcatgg     1140

cgactggcgg gtgtgccgga tgtctggagc tggcttgaaa tgataaccac cgcattacaa     1200

caaaaaagag aaaataacag gagtgatgac tatgagtcgt ttagtcgtag tatctaaaaa     1260

aaagtcttaa taaataaaaa atagtggttt gatagtgggg aataattttc cttctgtcaa     1320

atcatttttt attattgtgg tataataata aggaaaaatg ataaggggat agatacaaat     1380

gtgtggaatt gtcggcttta ttgaccggat cgatcaaaat gataaatcaa aaactttaga     1440

agaaatgatg gatacaatcg ctcaccgagg tccaagtagt tcaggtgaat ttattgacga     1500

aggagcagca attggttttc gtcgcctgag tattattgac cttgagggtg gagatcaacc     1560

tatctttaat gaagataaaa ctaaacttat aacctttaat ggcgaaattt ataatttccg     1620

tgaattgcgt gaagacctta tctctaaagg tcatgatttt actactcatg ctgatacaga     1680

agtgctttta catggttacg                                                 1700


<210>  20
<211>  1929
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Genetically engineered construct


<220>
<221>  MISC
<222>  (389)..(1929)
<223>  Coding sequence of ptcC with insertion of tga30 at codon position
       30 of 446 (tga30) along with an EcoRI site to disrupt ptcC gene

<400>  20
caccgaatta acacgcatta tgacttaacg aggcaattac ctacggtcta tttgacagaa       60

cgtttaacag atctcgttta tcaacgttcc aactaaaaaa gccaatctgg cttttttcta      120

tgctctgtcg ttcctaatgg tttgatatct aaaagtaaaa aagttaaatt gagataacaa      180

atataattat caaggctgaa cctcgcaagc ttaaaggaaa cttatatgac aattttggta      240

caggagtctt caaaagtggc acagaaccaa agtgatggaa aaataagaaa ctgctttctt      300

tactttgcct attaatgcta taatgaaaat gtagaaaaga tggacgtgaa accagttcat      360

caaaaaaagt aaaggagact gttcaaccat gaacaatttt attcaaaaca aaatcatgcc      420

tccaatgatg aaatttttga atacccgtgc agtcacggca atcaaaaatg gtatgtgaat      480

tcctatccca tttatcatta ttggttcagt attcttgatt cttggtcaac tgccattcca      540

agcaggacaa gacttcatga acaaaatcaa attgggccca ctctttttac aaattaataa      600

gtcattgttc acatcttgtt gcaaccagac acaatccatc aagtaacaag tgttactgac      660

ccaactaaaa catcaacagc atttcaagta ggtggtgtca ttgaccgagc ttggttaggt      720

gggaaaggga tggttctctc aatcatcgtt ggactcttag taggttggat ttacactggc      780

tttatgcgtc ggaacatcac aatcaaaatg ccagaacaag ttccagaaaa cgttgccgca      840

tcatttactt cacttgtacc tgcaggagca atcattacaa tggctggtgt ggttcatgga      900

atcacaacga ttggcttcaa cacaactttt taagatttgc gctttgccaa ctgtacaccc      960

aacctcggtt tattgtcgaa cctcccgctt gtgccgccat ctgcatatag atcccggtca     1020

gtccgtcaca ttctgccaat tgagtatcct cgaagtctta ttccacgtgc tcaaagcaag     1080

ggtatcgtac agtgataacc gcctcgtgca gatccaaatt ctcgattaac actcaagtac     1140

tgatttttat catcaggtaa ctaaaaactc acaatttgaa gcaccagcga gaatcgttct     1200

attctctagc ttcgcaacat cgacagttgt aatggcataa cttcggcatt catagtggct     1260

gagtttagcg gactaagcga aaaactggtc gttagatctt cctcaccatg attttacaag     1320

aaaggtgaac tcaatttgac ggcggtaaag ttagatggct acgcgcgaca agtctccgta     1380

tcgtcatgaa attagcgaag aggtaatggc aaagcttggc tacgaataca ggagcgcgct     1440

gtgattacag tagggttagg atagcgaaaa cgttcaacgt ggatagactc ttatcggcac     1500

acgatcatat gcttccaagg ttcccaaggc gaattactag ggtgcacaga gctacgagta     1560

cgctgtccgg cttgattcgc tcgtacatcc actgttcaaa aagctccgat accgacgatc     1620

actctcgatc tctgtgtggg acgcacttat tgtggaatca gtcaaccagt gaagcattca     1680

catgtacgtg gtacggcacg ccgtggtatg ttagcgttcc ctgcgccgca agtaaaccct     1740

tcagctgtca cctcctatag taacacgctc gcatgcagag cctagcacct tagctctgag     1800

ttgcctgccg gaaggatata ttctgtatgt gattaaagcg aagtcaaagt aaacccccca     1860

catgcagacc tgggtaaatt ctcactcagt tgaaacgtag gggccaatac gtgtgtcctt     1920

gatactact                                                             1929


<210>  21
<211>  213
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Genetically engineered


<220>
<221>  MISC_FEATURE
<222>  (30)..(30)
<223>  Site of disruption of ptcC gene coding sequence by insertion of 
       tga and EcoRI site at codon 30 of ptcC gene

<400>  21

Met Asn Asn Phe Ile Gln Asn Lys Ile Met Pro Pro Met Met Lys Phe 
1               5                   10                  15      


Leu Asn Thr Arg Ala Val Thr Ala Ile Lys Asn Gly Met Xaa Ile Pro 
            20                  25                  30          


Ile Pro Phe Ile Ile Ile Gly Ser Val Phe Leu Ile Leu Gly Gln Leu 
        35                  40                  45              


Pro Phe Gln Ala Gly Gln Asp Phe Met Asn Lys Ile Lys Leu Gly Pro 
    50                  55                  60                  


Leu Phe Leu Gln Ile Asn Asn Ala Ser Phe Gly Ile Met Ala Leu Leu 
65                  70                  75                  80  


Ala Val Phe Gly Ile Ala Tyr Ala Trp Val Arg Asp Ala Gly Tyr Glu 
                85                  90                  95      


Gly Val Pro Ala Gly Leu Thr Gly Val Ile Val His Ile Leu Leu Gln 
            100                 105                 110         


Pro Asp Thr Ile His Gln Val Thr Ser Val Thr Asp Pro Thr Lys Thr 
        115                 120                 125             


Ser Thr Ala Phe Gln Val Gly Gly Val Ile Asp Arg Ala Trp Leu Gly 
    130                 135                 140                 


Gly Lys Gly Met Val Leu Ser Ile Ile Val Gly Leu Leu Val Gly Trp 
145                 150                 155                 160 


Ile Tyr Thr Gly Phe Met Arg Arg Asn Ile Thr Ile Lys Met Pro Glu 
                165                 170                 175     


Gln Val Pro Glu Asn Val Ala Ala Ser Phe Thr Ser Leu Val Pro Ala 
            180                 185                 190         


Gly Ala Ile Ile Thr Met Ala Gly Val Val His Gly Ile Thr Thr Ile 
        195                 200                 205             


Gly Phe Asn Thr Thr 
    210             


<210>  22
<211>  500
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Genetically engineered construct

<400>  22
tcagctaacg gagctcaact tgttaaaact gtatcttggt acgataacga aatgtcatac       60

acttcaaacc ttgttcgtac acttgcatac ttcgctaaaa tcgctaaata aggaggaaaa      120

aatgaagaag aaaatcatta gtgccatctt aatgtctaca gtgattcttt cagctgcagc      180

tcctttatca ggcgtttatg catttgtgaa ccaacacctg tgcggctcac acctggtgga      240

agctctctac ctagtgtgcg gggaacgagg cttcttctac acacccaaga cccgccggga      300

ggcagaggac ctgcaggtgg ggcaggtgga gctgggcggg ggccctggtg caggcagcct      360

gcagcccttg gccctggagg ggtccctgca gaagcgtggc attgtggaac aatgctgtac      420

cagcatctgc tccctctacc agctggagaa ctactgcaac taattttccg attttaacgg      480

tataaaaacc agtcttcggg                                                  500


<210>  23
<211>  1000
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Genetically engineered construct


<220>
<221>  CDS
<222>  (312)..(872)
<223>  Gene encoding fusion of SSusp45 (nt 312-392) and hIL-10 (nt. 
       393-876)

<400>  23
aatccaatga cggcacttct tccttggacg acaccagcac ctgtgagaat ggccatttca       60

ggtggacttc catttttgat tatttttgca atctgtttag tcttgaatgt tcttatttac      120

tacccattct ttaaggtggc gtataataaa gctttagaag aagaaaaagc agctgttgaa      180

ttagagggtt cagaaactgc ctgatggaaa acgccttaaa atggcatttt gacttgcaaa      240

ctgggctaag atttgctaaa atgaaaaatg cctatgttta aggtaaaaaa caaatggagg      300

acatttctaa a atg aaa aaa aag att atc tca gct att tta atg tct aca       350
             Met Lys Lys Lys Ile Ile Ser Ala Ile Leu Met Ser Thr          
             1               5                   10                       

gtg ata ctt tct gct gca gcc ccg ttg tca ggt gtt tac gcc tca gct        398
Val Ile Leu Ser Ala Ala Ala Pro Leu Ser Gly Val Tyr Ala Ser Ala           
    15                  20                  25                            

ggt caa ggt act caa tca gaa aac tca tgt act cac ttt cca ggt aac        446
Gly Gln Gly Thr Gln Ser Glu Asn Ser Cys Thr His Phe Pro Gly Asn           
30                  35                  40                  45            

ttg cca aac atg ctt cgt gat ttg cgt gat gct ttt tca cgt gtt aaa        494
Leu Pro Asn Met Leu Arg Asp Leu Arg Asp Ala Phe Ser Arg Val Lys           
                50                  55                  60                

act ttt ttt caa atg aaa gat caa ctt gat aac ttg ctt ttg aaa gaa        542
Thr Phe Phe Gln Met Lys Asp Gln Leu Asp Asn Leu Leu Leu Lys Glu           
            65                  70                  75                    

tca ctt ttg gaa gat ttt aaa ggt tac ctt ggt tgt caa gct ttg tca        590
Ser Leu Leu Glu Asp Phe Lys Gly Tyr Leu Gly Cys Gln Ala Leu Ser           
        80                  85                  90                        

gaa atg atc caa ttt tac ctt gaa gaa gtt atg cca caa gct gaa aac        638
Glu Met Ile Gln Phe Tyr Leu Glu Glu Val Met Pro Gln Ala Glu Asn           
    95                  100                 105                           

caa gat cca gat atc aaa gct cac gtt aac tca ttg ggt gaa aac ctt        686
Gln Asp Pro Asp Ile Lys Ala His Val Asn Ser Leu Gly Glu Asn Leu           
110                 115                 120                 125           

aaa act ttg cgt ctt cgt ttg cgt cgt tgt cac cgt ttt ctt cca tgt        734
Lys Thr Leu Arg Leu Arg Leu Arg Arg Cys His Arg Phe Leu Pro Cys           
                130                 135                 140               

gaa aac aaa tca aaa gct gtt gaa caa gtt aaa aac gct ttt aac aaa        782
Glu Asn Lys Ser Lys Ala Val Glu Gln Val Lys Asn Ala Phe Asn Lys           
            145                 150                 155                   

ttg caa gaa aaa ggt atc tac aaa gct atg tca gaa ttt gat atc ttt        830
Leu Gln Glu Lys Gly Ile Tyr Lys Ala Met Ser Glu Phe Asp Ile Phe           
        160                 165                 170                       

atc aac tac atc gaa gct tac atg act atg aaa atc cgt aac                872
Ile Asn Tyr Ile Glu Ala Tyr Met Thr Met Lys Ile Arg Asn                   
    175                 180                 185                           

taactagaat taatctataa gttactgaca aaactgtcag taactttttt tgtgggaaaa      932

atgtattttt atgaccgtaa agaatctgtc agtagaagtc tgaaattcgt ttaaaaatcg      992

actagaat                                                              1000


<210>  24
<211>  187
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic Construct

<400>  24

Met Lys Lys Lys Ile Ile Ser Ala Ile Leu Met Ser Thr Val Ile Leu 
1               5                   10                  15      


Ser Ala Ala Ala Pro Leu Ser Gly Val Tyr Ala Ser Ala Gly Gln Gly 
            20                  25                  30          


Thr Gln Ser Glu Asn Ser Cys Thr His Phe Pro Gly Asn Leu Pro Asn 
        35                  40                  45              


Met Leu Arg Asp Leu Arg Asp Ala Phe Ser Arg Val Lys Thr Phe Phe 
    50                  55                  60                  


Gln Met Lys Asp Gln Leu Asp Asn Leu Leu Leu Lys Glu Ser Leu Leu 
65                  70                  75                  80  


Glu Asp Phe Lys Gly Tyr Leu Gly Cys Gln Ala Leu Ser Glu Met Ile 
                85                  90                  95      


Gln Phe Tyr Leu Glu Glu Val Met Pro Gln Ala Glu Asn Gln Asp Pro 
            100                 105                 110         


Asp Ile Lys Ala His Val Asn Ser Leu Gly Glu Asn Leu Lys Thr Leu 
        115                 120                 125             


Arg Leu Arg Leu Arg Arg Cys His Arg Phe Leu Pro Cys Glu Asn Lys 
    130                 135                 140                 


Ser Lys Ala Val Glu Gln Val Lys Asn Ala Phe Asn Lys Leu Gln Glu 
145                 150                 155                 160 


Lys Gly Ile Tyr Lys Ala Met Ser Glu Phe Asp Ile Phe Ile Asn Tyr 
                165                 170                 175     


Ile Glu Ala Tyr Met Thr Met Lys Ile Arg Asn 
            180                 185         


