			SEQUENCE LISTING

<110> Codexis, Inc.
      Borra-Garske, Margie Tabuga
      Alvizo, Oscar
      Mayo, Melissa Ann
      Jenne, Stephan
      Sowell-Kantz, Auric Anthony
      Molinaro, Carmela
 
<120> ENGINEERED IMINE REDUCTASES AND METHODS FOR THE REDUCTIVE AMINATION OF KETONE AND AMINE COMPOUNDS
 
<130> CX2-151USP1
 
<140> Not Yet Assigned
<141> Submitted Herewith
 
 
<160> 270
 
<210> 1
<211> 879
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Imine reductase from Pseudonocardia spinosispora  
 
<400> 1
atgagtgatt cccccgtacc tgtgactgtt ctgggtcttg gtctgatggg tcaggcctta 60
gcgggagcgt ttctggcagc cggtcaccct acgacggttt ggaatcgcag cgcagctaaa 120
gctgatgaac tggttggtcg cggtgcaacc ctggccgcgt ctcccgcggc tgccgtcgca 180
gcgggtagtc tgatcgttgt ttgcgtcaca gatcatcgtg cggtgcgcga actgctggaa 240
ccacttggcg atgcactgcc aggtcgcgtt ctggtcaacc tgacgagcgg tacgtcgcgt 300
gaagcccgtg agaacgcaga acatactgcg gctcagggag ccacctacct ggatggtgtg 360
attctgacca ttccaagcgg cattggcacc ccagaagcta ccattctgta ttcgggaccg 420
cgcagtacct tcgatgaaca tgaaagcgca ctgcgcgcat taggtccggg ggcgaccttt 480
gtaggggaag atcacggact ggctgcactg tatgatgcag caatgctgag cgtcatgtgg 540
ggcctgctga atggtttcct gcatggaacc gcactgctgg gaaccgcggg cgtttcggca 600
acctctgtgg ttccgatgct gggtcaaggt atcgaagcgg tgaccggttg gattgcgcgc 660
tacgcagatc agattgatgc cggtgtctat ccagcagacg acgccacgat tgatacccag 720
cgtgctgcaa tgcagcacct ggtcgaggag agccgtgcgg cgggcatcaa tggcgaactt 780
ccggcgtttg tgaccaaact ggccgatcgc gcagtggcag aaggtcgtgg tggtgactct 840
tacgctgcac tgattgaaca gtttcgcaag tcagcttaa                        879

 
<210> 2
<211> 292
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Imine reductase from Pseudonocardia spinosispora  
 
<400> 2
Met Ser Asp Ser Pro Val Pro Val Thr Val Leu Gly Leu Gly Leu Met 
1               5                   10                  15      
Gly Gln Ala Leu Ala Gly Ala Phe Leu Ala Ala Gly His Pro Thr Thr 
            20                  25                  30          
Val Trp Asn Arg Ser Ala Ala Lys Ala Asp Glu Leu Val Gly Arg Gly 
        35                  40                  45              
Ala Thr Leu Ala Ala Ser Pro Ala Ala Ala Val Ala Ala Gly Ser Leu 
    50                  55                  60                  
Ile Val Val Cys Val Thr Asp His Arg Ala Val Arg Glu Leu Leu Glu 
65                  70                  75                  80  
Pro Leu Gly Asp Ala Leu Pro Gly Arg Val Leu Val Asn Leu Thr Ser 
                85                  90                  95      
Gly Thr Ser Arg Glu Ala Arg Glu Asn Ala Glu His Thr Ala Ala Gln 
            100                 105                 110         
Gly Ala Thr Tyr Leu Asp Gly Val Ile Leu Thr Ile Pro Ser Gly Ile 
        115                 120                 125             
Gly Thr Pro Glu Ala Thr Ile Leu Tyr Ser Gly Pro Arg Ser Thr Phe 
    130                 135                 140                 
Asp Glu His Glu Ser Ala Leu Arg Ala Leu Gly Pro Gly Ala Thr Phe 
145                 150                 155                 160 
Val Gly Glu Asp His Gly Leu Ala Ala Leu Tyr Asp Ala Ala Met Leu 
                165                 170                 175     
Ser Val Met Trp Gly Leu Leu Asn Gly Phe Leu His Gly Thr Ala Leu 
            180                 185                 190         
Leu Gly Thr Ala Gly Val Ser Ala Thr Ser Val Val Pro Met Leu Gly 
        195                 200                 205             
Gln Gly Ile Glu Ala Val Thr Gly Trp Ile Ala Arg Tyr Ala Asp Gln 
    210                 215                 220                 
Ile Asp Ala Gly Val Tyr Pro Ala Asp Asp Ala Thr Ile Asp Thr Gln 
225                 230                 235                 240 
Arg Ala Ala Met Gln His Leu Val Glu Glu Ser Arg Ala Ala Gly Ile 
                245                 250                 255     
Asn Gly Glu Leu Pro Ala Phe Val Thr Lys Leu Ala Asp Arg Ala Val 
            260                 265                 270         
Ala Glu Gly Arg Gly Gly Asp Ser Tyr Ala Ala Leu Ile Glu Gln Phe 
        275                 280                 285             
Arg Lys Ser Ala                                                 
    290                                                         

 
<210> 3
<211> 879
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Imine reductase variant from Pseudonocardia spinosispora  
 
<400> 3
atgagtgatt cccccgtacc tgtgactgtt ctgggtcttg gtctgatggg tcaggcctta 60
gcgggagcgt ttctggcagc cggtcaccct acgacggttt ggaatcgcag cgcagctaaa 120
gctgatgaac tggttggtcg cggtgcaacc ctggccgcgt ctcccgcggc tgccgtcgca 180
gcgggtagtc tgatcgttgt ttgcgtcaca gatcatcgtg cggtgcgcga actgctggaa 240
ccacttggcg atgcactgcc aggtcgcgtt ctggtcaacc tgacgagcgg tacgtcgcgt 300
gaagcccgtg agaacgcaga acatactgcg gctcagggag ccacctacct ggatggtgtg 360
attctgggta ttccaagcgg cattggcacc ccagaagcta ccattctgta ttcgggaccg 420
cgcagtacct tcgatgaaca tgaaagcgca ctgcgcgcat taggtccggg ggcgaccttt 480
gtaggggaag atcacggact ggctgcactg tatgatgcag caatgctgag cgtcatgtgg 540
ggcctgctga atggtttcct gcatggaacc gcactgctgg gaaccgcggg cgtttcggca 600
acctctgtgg ttccgatgct gggtcaaggt atcgaagcgg tgaccggttg gattgcgcgc 660
tacgcagatc agattgatgc cggtgtctat ccagcagacg acgccacgat tgatacccag 720
cgtgctgcaa tgcagcacct ggtcgaggag agccgtgcgg cgggcatcaa tggcgaactt 780
ccggcgtttg tgaccaaact ggccgatcgc gcagtggcag aaggtcgtgg tggtgactct 840
tacgctgcac tgattgaaca gtttcgcaag tcagcttaa                        879

 
<210> 4
<211> 292
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Imine reductase variant from Pseudonocardia spinosispora  
 
<400> 4
Met Ser Asp Ser Pro Val Pro Val Thr Val Leu Gly Leu Gly Leu Met 
1               5                   10                  15      
Gly Gln Ala Leu Ala Gly Ala Phe Leu Ala Ala Gly His Pro Thr Thr 
            20                  25                  30          
Val Trp Asn Arg Ser Ala Ala Lys Ala Asp Glu Leu Val Gly Arg Gly 
        35                  40                  45              
Ala Thr Leu Ala Ala Ser Pro Ala Ala Ala Val Ala Ala Gly Ser Leu 
    50                  55                  60                  
Ile Val Val Cys Val Thr Asp His Arg Ala Val Arg Glu Leu Leu Glu 
65                  70                  75                  80  
Pro Leu Gly Asp Ala Leu Pro Gly Arg Val Leu Val Asn Leu Thr Ser 
                85                  90                  95      
Gly Thr Ser Arg Glu Ala Arg Glu Asn Ala Glu His Thr Ala Ala Gln 
            100                 105                 110         
Gly Ala Thr Tyr Leu Asp Gly Val Ile Leu Gly Ile Pro Ser Gly Ile 
        115                 120                 125             
Gly Thr Pro Glu Ala Thr Ile Leu Tyr Ser Gly Pro Arg Ser Thr Phe 
    130                 135                 140                 
Asp Glu His Glu Ser Ala Leu Arg Ala Leu Gly Pro Gly Ala Thr Phe 
145                 150                 155                 160 
Val Gly Glu Asp His Gly Leu Ala Ala Leu Tyr Asp Ala Ala Met Leu 
                165                 170                 175     
Ser Val Met Trp Gly Leu Leu Asn Gly Phe Leu His Gly Thr Ala Leu 
            180                 185                 190         
Leu Gly Thr Ala Gly Val Ser Ala Thr Ser Val Val Pro Met Leu Gly 
        195                 200                 205             
Gln Gly Ile Glu Ala Val Thr Gly Trp Ile Ala Arg Tyr Ala Asp Gln 
    210                 215                 220                 
Ile Asp Ala Gly Val Tyr Pro Ala Asp Asp Ala Thr Ile Asp Thr Gln 
225                 230                 235                 240 
Arg Ala Ala Met Gln His Leu Val Glu Glu Ser Arg Ala Ala Gly Ile 
                245                 250                 255     
Asn Gly Glu Leu Pro Ala Phe Val Thr Lys Leu Ala Asp Arg Ala Val 
            260                 265                 270         
Ala Glu Gly Arg Gly Gly Asp Ser Tyr Ala Ala Leu Ile Glu Gln Phe 
        275                 280                 285             
Arg Lys Ser Ala                                                 
    290                                                         

 
<210> 5
<211> 879
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Imine reductase variant from Pseudonocardia spinosispora  
 
<400> 5
atgagtgatt cccccgtacc tgtgactgtt ctgggtcttg gtctgatggg tcaggcctta 60
gcgggagcgt ttctggcagc cggtcaccct acgacggttt ggaatcgcag cgcagctaaa 120
gctgatgaac tggttggtcg cggtgcaacc ctggccgcgt ctcccgcggc tgccgtcgca 180
gcgggtagtc tgatcgttgt ttgcgtcaca gatcatcgtg cggtgcgcga actgctggaa 240
ccacttggcg atgcactgcc aggtcgcgtt ctggtcaacc tgacgagcgg tacgtcgcgt 300
gaagcccgtg agaacgcaga acatactgcg gctcagggag ccacctacct ggatggtgtg 360
attctgacca ttccaagcgg cattggcacc ccacatgcta ccattctgta ttcgggaccg 420
cgcagtacct tcgatgaaca tgaaagcgca ctgcgcgcat taggtccggg ggcgaccttt 480
gtaggggaag atcacggact ggctgcactg tatgatgcag caatgctgag cgtcatgtgg 540
ggcctgctga atggtttcct gcatggaacc gcactgctgg gaaccgcggg cgtttcggca 600
acctctgtgg ttccgatgct gggtcaaggt atcgaagcgg tgaccggttg gattgcgcgc 660
tacgcagatc agattgatgc cggtgtctat ccagcagacg acgccacgat tgatacccag 720
cgtgctgcaa tgcagcacct ggtcgaggag agccgtgcgg cgggcatcaa tggcgaactt 780
ccggcgtttg tgaccaaact ggccgatcgc gcagtggcag aaggtcgtgg tggtgactct 840
tacgctgcac tgattgaaca gtttcgcaag tcagcttaa                        879

 
<210> 6
<211> 292
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Imine reductase variant from Pseudonocardia spinosispora  
 
<400> 6
Met Ser Asp Ser Pro Val Pro Val Thr Val Leu Gly Leu Gly Leu Met 
1               5                   10                  15      
Gly Gln Ala Leu Ala Gly Ala Phe Leu Ala Ala Gly His Pro Thr Thr 
            20                  25                  30          
Val Trp Asn Arg Ser Ala Ala Lys Ala Asp Glu Leu Val Gly Arg Gly 
        35                  40                  45              
Ala Thr Leu Ala Ala Ser Pro Ala Ala Ala Val Ala Ala Gly Ser Leu 
    50                  55                  60                  
Ile Val Val Cys Val Thr Asp His Arg Ala Val Arg Glu Leu Leu Glu 
65                  70                  75                  80  
Pro Leu Gly Asp Ala Leu Pro Gly Arg Val Leu Val Asn Leu Thr Ser 
                85                  90                  95      
Gly Thr Ser Arg Glu Ala Arg Glu Asn Ala Glu His Thr Ala Ala Gln 
            100                 105                 110         
Gly Ala Thr Tyr Leu Asp Gly Val Ile Leu Thr Ile Pro Ser Gly Ile 
        115                 120                 125             
Gly Thr Pro His Ala Thr Ile Leu Tyr Ser Gly Pro Arg Ser Thr Phe 
    130                 135                 140                 
Asp Glu His Glu Ser Ala Leu Arg Ala Leu Gly Pro Gly Ala Thr Phe 
145                 150                 155                 160 
Val Gly Glu Asp His Gly Leu Ala Ala Leu Tyr Asp Ala Ala Met Leu 
                165                 170                 175     
Ser Val Met Trp Gly Leu Leu Asn Gly Phe Leu His Gly Thr Ala Leu 
            180                 185                 190         
Leu Gly Thr Ala Gly Val Ser Ala Thr Ser Val Val Pro Met Leu Gly 
        195                 200                 205             
Gln Gly Ile Glu Ala Val Thr Gly Trp Ile Ala Arg Tyr Ala Asp Gln 
    210                 215                 220                 
Ile Asp Ala Gly Val Tyr Pro Ala Asp Asp Ala Thr Ile Asp Thr Gln 
225                 230                 235                 240 
Arg Ala Ala Met Gln His Leu Val Glu Glu Ser Arg Ala Ala Gly Ile 
                245                 250                 255     
Asn Gly Glu Leu Pro Ala Phe Val Thr Lys Leu Ala Asp Arg Ala Val 
            260                 265                 270         
Ala Glu Gly Arg Gly Gly Asp Ser Tyr Ala Ala Leu Ile Glu Gln Phe 
        275                 280                 285             
Arg Lys Ser Ala                                                 
    290                                                         

 
<210> 7
<211> 879
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Imine reductase variant from Pseudonocardia spinosispora  
 
<400> 7
atgagtgatt cccccgtacc tgtgactgtt ctgggtcttg gtctgatggg tcaggcctta 60
gcgggagcgt ttctggcagc cggtcaccct acgacggttt ggaatcgcag cgcagctaaa 120
gctgatgaac tggttggtcg cggtgcaacc ctggccgcgt ctcccgcggc tgccgtcgca 180
gcgggtagtc tgatcgttgt ttgcgtcaca gatcatcgtg cggtgcgcga actgctggaa 240
ccacttggcg atgcactgcc aggtcgcgtt ctggtcaacc tgacgagcgg tacgtcgcgt 300
gaagcccgtg agaacgcaga acatactgcg gctcagggag ccacctacct ggatggtgtg 360
attctgaccg caccaagcgg cattggcacc ccagaagcta ccattctgta ttcgggaccg 420
cgcagtacct tcgatgaaca tgaaagcgca ctgcgcgcat taggtccggg ggcgaccttt 480
gtaggggaag atcacggact ggctgcactg tatgatgcag caatgctgag catcatgtgg 540
ggcctgctga atggtttcct gcatggaacc gcactgctgg gaaccgcggg cgtttcggca 600
acctctgtgg ttccgatgct gggtcaaggt atcgaagcgg tgaccggttg gattgcgcgc 660
tacgcagatc agattgatgc cggtgtctat ccagcagacg acgccacgat tgatacccag 720
cgtgctgcaa tgcagcacct ggtcgaggag agccgtgcgg cgggcatcaa tggcgaactt 780
ccggcgtttg tgaccaaact ggccgatcgc gcagtggcag aaggtcgtgg tggtgactct 840
tacgctgcac tgattgaaca gtttcgcaag tcagcttaa                        879

 
<210> 8
<211> 292
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Imine reductase variant from Pseudonocardia spinosispora  
 
<400> 8
Met Ser Asp Ser Pro Val Pro Val Thr Val Leu Gly Leu Gly Leu Met 
1               5                   10                  15      
Gly Gln Ala Leu Ala Gly Ala Phe Leu Ala Ala Gly His Pro Thr Thr 
            20                  25                  30          
Val Trp Asn Arg Ser Ala Ala Lys Ala Asp Glu Leu Val Gly Arg Gly 
        35                  40                  45              
Ala Thr Leu Ala Ala Ser Pro Ala Ala Ala Val Ala Ala Gly Ser Leu 
    50                  55                  60                  
Ile Val Val Cys Val Thr Asp His Arg Ala Val Arg Glu Leu Leu Glu 
65                  70                  75                  80  
Pro Leu Gly Asp Ala Leu Pro Gly Arg Val Leu Val Asn Leu Thr Ser 
                85                  90                  95      
Gly Thr Ser Arg Glu Ala Arg Glu Asn Ala Glu His Thr Ala Ala Gln 
            100                 105                 110         
Gly Ala Thr Tyr Leu Asp Gly Val Ile Leu Thr Ala Pro Ser Gly Ile 
        115                 120                 125             
Gly Thr Pro Glu Ala Thr Ile Leu Tyr Ser Gly Pro Arg Ser Thr Phe 
    130                 135                 140                 
Asp Glu His Glu Ser Ala Leu Arg Ala Leu Gly Pro Gly Ala Thr Phe 
145                 150                 155                 160 
Val Gly Glu Asp His Gly Leu Ala Ala Leu Tyr Asp Ala Ala Met Leu 
                165                 170                 175     
Ser Ile Met Trp Gly Leu Leu Asn Gly Phe Leu His Gly Thr Ala Leu 
            180                 185                 190         
Leu Gly Thr Ala Gly Val Ser Ala Thr Ser Val Val Pro Met Leu Gly 
        195                 200                 205             
Gln Gly Ile Glu Ala Val Thr Gly Trp Ile Ala Arg Tyr Ala Asp Gln 
    210                 215                 220                 
Ile Asp Ala Gly Val Tyr Pro Ala Asp Asp Ala Thr Ile Asp Thr Gln 
225                 230                 235                 240 
Arg Ala Ala Met Gln His Leu Val Glu Glu Ser Arg Ala Ala Gly Ile 
                245                 250                 255     
Asn Gly Glu Leu Pro Ala Phe Val Thr Lys Leu Ala Asp Arg Ala Val 
            260                 265                 270         
Ala Glu Gly Arg Gly Gly Asp Ser Tyr Ala Ala Leu Ile Glu Gln Phe 
        275                 280                 285             
Arg Lys Ser Ala                                                 
    290                                                         

 
<210> 9
<211> 879
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Imine reductase variant from Pseudonocardia spinosispora  
 
<400> 9
atgagtgatt cccccgtacc tgtgactgtt ctgggtcttg gtctgatggg tcaggcctta 60
gcgggagcgt ttctggcagc cggtcaccct acgacggttt ggaatcgcag cgcagctaaa 120
gctgatgaac tggttggtcg cggtgcaacc ctggccgcgt ctcccgcggc tgccgtcgca 180
gcgggtagtc tgatcgttgt ttgcgtcaca gatcatcgtg cggtgcgcga actgctggaa 240
ccacttggcg atgcactgcc aggtcgcgtt ctggtcaacc tgacgagcgg tacgtcgcgt 300
gaagcccgtg agaacgcaga acatactgcg gctcagggag ccacctacct ggatggtgtg 360
attctgaccg gtccaagcgg cattggcacc ccagaagcta ccattctgta ttcgggaccg 420
cgcagtacct tcgatgaaca tgaaagcgca ctgcgcgcat taggtccggg ggcgaccttt 480
gtaggggaag atcacggact ggctgcactg tatgatgcag caatgctgag cgtcatgtgg 540
ggcctgctga atggtttcct gcatggaacc gcactgctgg gaaccgcggg cgtttcggca 600
acctctgtgg ttccgatgct gggtcaaggt atcgaagcgg tgaccggttg gattgcgcgc 660
tacgcagatc agattgatgc cggtgtctat ccagcagacg acgccacgat tgatacccag 720
cgtgctgcaa tgcagcacct ggtcgaggag agccgtgcgg cgggcatcaa tggcgaactt 780
ccggcgtttg tgaccaaact ggccgatcgc gcagtggcag aaggtcgtgg tggtgactct 840
tacgctgcac tgattgaaca gtttcgcaag tcagcttaa                        879

 
<210> 10
<211> 292
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Imine reductase variant from Pseudonocardia spinosispora  
 
<400> 10
Met Ser Asp Ser Pro Val Pro Val Thr Val Leu Gly Leu Gly Leu Met 
1               5                   10                  15      
Gly Gln Ala Leu Ala Gly Ala Phe Leu Ala Ala Gly His Pro Thr Thr 
            20                  25                  30          
Val Trp Asn Arg Ser Ala Ala Lys Ala Asp Glu Leu Val Gly Arg Gly 
        35                  40                  45              
Ala Thr Leu Ala Ala Ser Pro Ala Ala Ala Val Ala Ala Gly Ser Leu 
    50                  55                  60                  
Ile Val Val Cys Val Thr Asp His Arg Ala Val Arg Glu Leu Leu Glu 
65                  70                  75                  80  
Pro Leu Gly Asp Ala Leu Pro Gly Arg Val Leu Val Asn Leu Thr Ser 
                85                  90                  95      
Gly Thr Ser Arg Glu Ala Arg Glu Asn Ala Glu His Thr Ala Ala Gln 
            100                 105                 110         
Gly Ala Thr Tyr Leu Asp Gly Val Ile Leu Thr Gly Pro Ser Gly Ile 
        115                 120                 125             
Gly Thr Pro Glu Ala Thr Ile Leu Tyr Ser Gly Pro Arg Ser Thr Phe 
    130                 135                 140                 
Asp Glu His Glu Ser Ala Leu Arg Ala Leu Gly Pro Gly Ala Thr Phe 
145                 150                 155                 160 
Val Gly Glu Asp His Gly Leu Ala Ala Leu Tyr Asp Ala Ala Met Leu 
                165                 170                 175     
Ser Val Met Trp Gly Leu Leu Asn Gly Phe Leu His Gly Thr Ala Leu 
            180                 185                 190         
Leu Gly Thr Ala Gly Val Ser Ala Thr Ser Val Val Pro Met Leu Gly 
        195                 200                 205             
Gln Gly Ile Glu Ala Val Thr Gly Trp Ile Ala Arg Tyr Ala Asp Gln 
    210                 215                 220                 
Ile Asp Ala Gly Val Tyr Pro Ala Asp Asp Ala Thr Ile Asp Thr Gln 
225                 230                 235                 240 
Arg Ala Ala Met Gln His Leu Val Glu Glu Ser Arg Ala Ala Gly Ile 
                245                 250                 255     
Asn Gly Glu Leu Pro Ala Phe Val Thr Lys Leu Ala Asp Arg Ala Val 
            260                 265                 270         
Ala Glu Gly Arg Gly Gly Asp Ser Tyr Ala Ala Leu Ile Glu Gln Phe 
        275                 280                 285             
Arg Lys Ser Ala                                                 
    290                                                         

 
<210> 11
<211> 879
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Imine reductase variant from Pseudonocardia spinosispora  
 
<400> 11
atgagtgatt cccccgtacc tgtgactgtt ctgggtcttg gtctgatggg tcaggcctta 60
gcgggagcgt ttctggcagc cggtcaccct acgacggttt ggaatcgcag cgcagctaaa 120
gctgatgaac tggttggtcg cggtgcaacc ctggccgcgt ctcccgcggc tgccgtcgca 180
gcgggtagtc tgatcgttgt ttgcgtcaca gatcatcgtg cggtgcgcga actgctggaa 240
ccacttggcg atgcactgcc aggtcgcgtt ctggtcaacc tgacgagcgg tacgtcgcgt 300
gaagcccgtg agaacgcaga acatactgcg gctcagggag ccacctacct ggatggtgtg 360
attctgacca ttccaagcgg cattggcacc ccagaagcta ccattctgta ttcgggaccg 420
cgcagtacct tcgatgaaca tgaaagcgca ctgcgcgcat taggtccggg ggcgaccttt 480
gtaggggaag atcacggact ggctgcactg tatgatcatg caatgctgag cgtcatgtgg 540
ggcctgctga atggtttcct gcatggaacc gcactgctgg gaaccgcggg cgtttcggca 600
acctctgtgg ttccgatgct gggtcaaggt atcgaagcgg tgaccggttg gattgcgcgc 660
tacgcagatc agattgatgc cggtgtctat ccagcagacg acgccacgat tgatacccag 720
cgtgctgcaa tgcagcacct ggtcgaggag agccgtgcgg cgggcatcaa tggcgaactt 780
ccggcgtttg tgaccaaact ggccgatcgc gcagtggcag aaggtcgtgg tggtgactct 840
tacgctgcac tgattgaaca gtttcgcaag tcagcttaa                        879

 
<210> 12
<211> 292
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Imine reductase variant from Pseudonocardia spinosispora  
 
<400> 12
Met Ser Asp Ser Pro Val Pro Val Thr Val Leu Gly Leu Gly Leu Met 
1               5                   10                  15      
Gly Gln Ala Leu Ala Gly Ala Phe Leu Ala Ala Gly His Pro Thr Thr 
            20                  25                  30          
Val Trp Asn Arg Ser Ala Ala Lys Ala Asp Glu Leu Val Gly Arg Gly 
        35                  40                  45              
Ala Thr Leu Ala Ala Ser Pro Ala Ala Ala Val Ala Ala Gly Ser Leu 
    50                  55                  60                  
Ile Val Val Cys Val Thr Asp His Arg Ala Val Arg Glu Leu Leu Glu 
65                  70                  75                  80  
Pro Leu Gly Asp Ala Leu Pro Gly Arg Val Leu Val Asn Leu Thr Ser 
                85                  90                  95      
Gly Thr Ser Arg Glu Ala Arg Glu Asn Ala Glu His Thr Ala Ala Gln 
            100                 105                 110         
Gly Ala Thr Tyr Leu Asp Gly Val Ile Leu Thr Ile Pro Ser Gly Ile 
        115                 120                 125             
Gly Thr Pro Glu Ala Thr Ile Leu Tyr Ser Gly Pro Arg Ser Thr Phe 
    130                 135                 140                 
Asp Glu His Glu Ser Ala Leu Arg Ala Leu Gly Pro Gly Ala Thr Phe 
145                 150                 155                 160 
Val Gly Glu Asp His Gly Leu Ala Ala Leu Tyr Asp His Ala Met Leu 
                165                 170                 175     
Ser Val Met Trp Gly Leu Leu Asn Gly Phe Leu His Gly Thr Ala Leu 
            180                 185                 190         
Leu Gly Thr Ala Gly Val Ser Ala Thr Ser Val Val Pro Met Leu Gly 
        195                 200                 205             
Gln Gly Ile Glu Ala Val Thr Gly Trp Ile Ala Arg Tyr Ala Asp Gln 
    210                 215                 220                 
Ile Asp Ala Gly Val Tyr Pro Ala Asp Asp Ala Thr Ile Asp Thr Gln 
225                 230                 235                 240 
Arg Ala Ala Met Gln His Leu Val Glu Glu Ser Arg Ala Ala Gly Ile 
                245                 250                 255     
Asn Gly Glu Leu Pro Ala Phe Val Thr Lys Leu Ala Asp Arg Ala Val 
            260                 265                 270         
Ala Glu Gly Arg Gly Gly Asp Ser Tyr Ala Ala Leu Ile Glu Gln Phe 
        275                 280                 285             
Arg Lys Ser Ala                                                 
    290                                                         

 
<210> 13
<211> 879
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Imine reductase variant from Pseudonocardia spinosispora  
 
<400> 13
atgagtgatt cccccgtacc tgtgactgtt ctgggtcttg gtctgatggg tcaggcctta 60
gcgggagcgt ttctggcagc cggtcaccct acgacggttt ggaatcgcag cgcagctaaa 120
gctgatgaac tggttggtcg cggtgcaacc ctggccgcgt ctcccgcggc tgccgtcgca 180
gcgggtagtc tgatcgttgt ttgcgtcaca gatcatcgtg cggtgcgcga actgctggaa 240
ccacttggcg atgcactgcc aggtcgcgtt ctggtcaacc tgacgagcgg tacgtcgcgt 300
gaagcccgtg agaacgcaga acatactgcg gctcagggag ccacctacct ggatggtgtg 360
attctgacca ttccaagcgg cattggcacc ccagaagcta ccattctgta ttcgggaccg 420
cgcagtacct tcgatgaaca tgaaagcgca ctgcgcgcat taggtccggg ggcgaccttt 480
gtaggggaag atcacggact ggctaatctg tatgatgcag caatgctgag cgtcatgtgg 540
ggcctgctga atggtttcct gcatggaacc gcactgctgg gaaccgcggg cgtttcggca 600
acctctgtgg ttccgatgct gggtcaaggt atcgaagcgg tgaccggttg gattgcgcgc 660
tacgcagatc agattgatgc cggtgtctat ccagcagacg acgccacgat tgatacccag 720
cgtgctgcaa tgcagcacct ggtcgaggag agccgtgcgg cgggcatcaa tggcgaactt 780
ccggcgtttg tgaccaaact ggccgatcgc gcagtggcag aaggtcgtgg tggtgactct 840
tacgctgcac tgattgaaca gtttcgcaag tcagcttaa                        879

 
<210> 14
<211> 292
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Imine reductase variant from Pseudonocardia spinosispora  
 
<400> 14
Met Ser Asp Ser Pro Val Pro Val Thr Val Leu Gly Leu Gly Leu Met 
1               5                   10                  15      
Gly Gln Ala Leu Ala Gly Ala Phe Leu Ala Ala Gly His Pro Thr Thr 
            20                  25                  30          
Val Trp Asn Arg Ser Ala Ala Lys Ala Asp Glu Leu Val Gly Arg Gly 
        35                  40                  45              
Ala Thr Leu Ala Ala Ser Pro Ala Ala Ala Val Ala Ala Gly Ser Leu 
    50                  55                  60                  
Ile Val Val Cys Val Thr Asp His Arg Ala Val Arg Glu Leu Leu Glu 
65                  70                  75                  80  
Pro Leu Gly Asp Ala Leu Pro Gly Arg Val Leu Val Asn Leu Thr Ser 
                85                  90                  95      
Gly Thr Ser Arg Glu Ala Arg Glu Asn Ala Glu His Thr Ala Ala Gln 
            100                 105                 110         
Gly Ala Thr Tyr Leu Asp Gly Val Ile Leu Thr Ile Pro Ser Gly Ile 
        115                 120                 125             
Gly Thr Pro Glu Ala Thr Ile Leu Tyr Ser Gly Pro Arg Ser Thr Phe 
    130                 135                 140                 
Asp Glu His Glu Ser Ala Leu Arg Ala Leu Gly Pro Gly Ala Thr Phe 
145                 150                 155                 160 
Val Gly Glu Asp His Gly Leu Ala Asn Leu Tyr Asp Ala Ala Met Leu 
                165                 170                 175     
Ser Val Met Trp Gly Leu Leu Asn Gly Phe Leu His Gly Thr Ala Leu 
            180                 185                 190         
Leu Gly Thr Ala Gly Val Ser Ala Thr Ser Val Val Pro Met Leu Gly 
        195                 200                 205             
Gln Gly Ile Glu Ala Val Thr Gly Trp Ile Ala Arg Tyr Ala Asp Gln 
    210                 215                 220                 
Ile Asp Ala Gly Val Tyr Pro Ala Asp Asp Ala Thr Ile Asp Thr Gln 
225                 230                 235                 240 
Arg Ala Ala Met Gln His Leu Val Glu Glu Ser Arg Ala Ala Gly Ile 
                245                 250                 255     
Asn Gly Glu Leu Pro Ala Phe Val Thr Lys Leu Ala Asp Arg Ala Val 
            260                 265                 270         
Ala Glu Gly Arg Gly Gly Asp Ser Tyr Ala Ala Leu Ile Glu Gln Phe 
        275                 280                 285             
Arg Lys Ser Ala                                                 
    290                                                         

 
<210> 15
<211> 879
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Imine reductase variant from Pseudonocardia spinosispora  
 
<400> 15
atgagtgatt cccccgtacc tgtgactgtt ctgggtcttg gtctgatggg tcaggcctta 60
gcgggagcgt ttctggcagc cggtcaccct acgacggttt ggaatcgcag cgcagctaaa 120
gctgatgaac tggttggtcg cggtgcaacc ctggccgcgt ctcccgcggc tgccgtcgca 180
gcgggtagtc tgatcgttgt ttgcgtcaca gatcatcgtg cggtgcgcga actgctggaa 240
ccacttggcg atgcactgcc aggtcgcgtt ctggtcaacc tgacgagcgg tacgtcgcgt 300
gaagcccgtg agaacgcaga acatactgcg gctcagggag ccacctacct ggatggtgtg 360
attctgacca ttccaagcgg cattggcacc ccagaagcta ccattctgta ttcgggaccg 420
cgcagtacct tcgatgaaca tgaaagcgca ctgcgcgcat taggtccggg ggcgaccttt 480
gtaggggaag atcacggact ggctgcactg tatgatgcag caatgctgag cgtcatgtgg 540
ggcctgctga atggtttcct gcatggaacc gcactgctgg gaaccgcggg cgtttcggca 600
acctctgtgg ttccgatgct gggtcaaggt atcgaagcgg tgaccggttg gattgcgcgc 660
tacgcagatc agattgatgc cggtgtctat ccagcagact tggccacgat tgatacccag 720
cgtgctgcaa tgcagcacct ggtcgaggag agccgtgcgg cgggcatcaa tggcgaactt 780
ccggcgtttg tgaccaaact ggccgatcgc gcagtggcag aaggtcgtgg tggtgactct 840
tacgctgcac tgattgaaca gtttcgcaag tcagcttaa                        879

 
<210> 16
<211> 292
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Imine reductase variant from Pseudonocardia spinosispora  
 
<400> 16
Met Ser Asp Ser Pro Val Pro Val Thr Val Leu Gly Leu Gly Leu Met 
1               5                   10                  15      
Gly Gln Ala Leu Ala Gly Ala Phe Leu Ala Ala Gly His Pro Thr Thr 
            20                  25                  30          
Val Trp Asn Arg Ser Ala Ala Lys Ala Asp Glu Leu Val Gly Arg Gly 
        35                  40                  45              
Ala Thr Leu Ala Ala Ser Pro Ala Ala Ala Val Ala Ala Gly Ser Leu 
    50                  55                  60                  
Ile Val Val Cys Val Thr Asp His Arg Ala Val Arg Glu Leu Leu Glu 
65                  70                  75                  80  
Pro Leu Gly Asp Ala Leu Pro Gly Arg Val Leu Val Asn Leu Thr Ser 
                85                  90                  95      
Gly Thr Ser Arg Glu Ala Arg Glu Asn Ala Glu His Thr Ala Ala Gln 
            100                 105                 110         
Gly Ala Thr Tyr Leu Asp Gly Val Ile Leu Thr Ile Pro Ser Gly Ile 
        115                 120                 125             
Gly Thr Pro Glu Ala Thr Ile Leu Tyr Ser Gly Pro Arg Ser Thr Phe 
    130                 135                 140                 
Asp Glu His Glu Ser Ala Leu Arg Ala Leu Gly Pro Gly Ala Thr Phe 
145                 150                 155                 160 
Val Gly Glu Asp His Gly Leu Ala Ala Leu Tyr Asp Ala Ala Met Leu 
                165                 170                 175     
Ser Val Met Trp Gly Leu Leu Asn Gly Phe Leu His Gly Thr Ala Leu 
            180                 185                 190         
Leu Gly Thr Ala Gly Val Ser Ala Thr Ser Val Val Pro Met Leu Gly 
        195                 200                 205             
Gln Gly Ile Glu Ala Val Thr Gly Trp Ile Ala Arg Tyr Ala Asp Gln 
    210                 215                 220                 
Ile Asp Ala Gly Val Tyr Pro Ala Asp Leu Ala Thr Ile Asp Thr Gln 
225                 230                 235                 240 
Arg Ala Ala Met Gln His Leu Val Glu Glu Ser Arg Ala Ala Gly Ile 
                245                 250                 255     
Asn Gly Glu Leu Pro Ala Phe Val Thr Lys Leu Ala Asp Arg Ala Val 
            260                 265                 270         
Ala Glu Gly Arg Gly Gly Asp Ser Tyr Ala Ala Leu Ile Glu Gln Phe 
        275                 280                 285             
Arg Lys Ser Ala                                                 
    290                                                         

 
<210> 17
<211> 879
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Imine reductase variant from Pseudonocardia spinosispora  
 
<400> 17
atgagtgatt cccccgtacc tgtgactgtt ctgggtcttg gtctgatggg tcaggcctta 60
gcgggagcgt ttctggcagc cggtcaccct acgacggttt ggaatcgcag cgcagctaaa 120
gctgatgaac tggttggtcg cggtgcaacc ctggccgcgt ctcccgcggc tgccgtcgca 180
gcgggtagtc tgatcgttgt ttgcgtcaca gatcatcgtg cggtgcgcga actgctggaa 240
ccacttggcg atgcactgcc aggtcgcgtt ctggtcaacc tgacgagcgg tacgtcgcgt 300
gaagcccgtg agaacgcaga acatactgcg gctcagggag ccacctacct ggatggtgtg 360
attctgacca ttccaagcgg cattggcacc ccagaagcta ccattctgta ttcgggaccg 420
cgcagtacct tcgatgaaca tgaaagcgca ctgcgcgcat taggtccggg ggcgaccttt 480
gtaggggaag atcacggact ggctgcactg tgggatgcag caatgctgag cgtcatgtgg 540
ggcctgctga atggtttcct gcatggaacc gcactgctgg gaaccgcggg cgtttcggca 600
acctctgtgg ttccgatgct gggtcaaggt atcgaagcgg tgaccggttg gattgcgcgc 660
tacgcagatc agattgatgc cggtgtctat ccagcagacg acgccacgat tgatacccag 720
cgtgctgcaa tgcagcacct ggtcgaggag agccgtgcgg cgggcatcaa tggcgaactt 780
ccggcgtttg tgaccaaact ggccgatcgc gcagtggcag aaggtcgtgg tggtgactct 840
tacgctgcac tgattgaaca gtttcgcaag tcagcttaa                        879

 
<210> 18
<211> 292
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Imine reductase variant from Pseudonocardia spinosispora  
 
<400> 18
Met Ser Asp Ser Pro Val Pro Val Thr Val Leu Gly Leu Gly Leu Met 
1               5                   10                  15      
Gly Gln Ala Leu Ala Gly Ala Phe Leu Ala Ala Gly His Pro Thr Thr 
            20                  25                  30          
Val Trp Asn Arg Ser Ala Ala Lys Ala Asp Glu Leu Val Gly Arg Gly 
        35                  40                  45              
Ala Thr Leu Ala Ala Ser Pro Ala Ala Ala Val Ala Ala Gly Ser Leu 
    50                  55                  60                  
Ile Val Val Cys Val Thr Asp His Arg Ala Val Arg Glu Leu Leu Glu 
65                  70                  75                  80  
Pro Leu Gly Asp Ala Leu Pro Gly Arg Val Leu Val Asn Leu Thr Ser 
                85                  90                  95      
Gly Thr Ser Arg Glu Ala Arg Glu Asn Ala Glu His Thr Ala Ala Gln 
            100                 105                 110         
Gly Ala Thr Tyr Leu Asp Gly Val Ile Leu Thr Ile Pro Ser Gly Ile 
        115                 120                 125             
Gly Thr Pro Glu Ala Thr Ile Leu Tyr Ser Gly Pro Arg Ser Thr Phe 
    130                 135                 140                 
Asp Glu His Glu Ser Ala Leu Arg Ala Leu Gly Pro Gly Ala Thr Phe 
145                 150                 155                 160 
Val Gly Glu Asp His Gly Leu Ala Ala Leu Trp Asp Ala Ala Met Leu 
                165                 170                 175     
Ser Val Met Trp Gly Leu Leu Asn Gly Phe Leu His Gly Thr Ala Leu 
            180                 185                 190         
Leu Gly Thr Ala Gly Val Ser Ala Thr Ser Val Val Pro Met Leu Gly 
        195                 200                 205             
Gln Gly Ile Glu Ala Val Thr Gly Trp Ile Ala Arg Tyr Ala Asp Gln 
    210                 215                 220                 
Ile Asp Ala Gly Val Tyr Pro Ala Asp Asp Ala Thr Ile Asp Thr Gln 
225                 230                 235                 240 
Arg Ala Ala Met Gln His Leu Val Glu Glu Ser Arg Ala Ala Gly Ile 
                245                 250                 255     
Asn Gly Glu Leu Pro Ala Phe Val Thr Lys Leu Ala Asp Arg Ala Val 
            260                 265                 270         
Ala Glu Gly Arg Gly Gly Asp Ser Tyr Ala Ala Leu Ile Glu Gln Phe 
        275                 280                 285             
Arg Lys Ser Ala                                                 
    290                                                         

 
<210> 19
<211> 879
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Imine reductase variant from Pseudonocardia spinosispora  
 
<400> 19
atgagtgatt cccccgtacc tgtgactgtt ctgggtcttg gtctgatggg tcaggcctta 60
gcgggagcgt ttctggcagc cggtcaccct acgacggttt ggaatcgcag cgcagctaaa 120
gctgatgaac tggttggtcg cggtgcaacc ctggccgcgt ctcccgcggc tgccgtcgca 180
gcgggtagtc tgatcgttgt ttgcgtcaca gatcatcgtg cggtgcgcga actgctggaa 240
ccacttggcg atgcactgcc aggtcgcgtt ctggtcaacc tgacgagcgg tacgtcgcgt 300
gaagcccgtg agaacgcaga acatactgcg gctcagggag ccacctacct ggatggtgtg 360
attctgacca ttccaagcgg cattggcacc ccagaagcta ccattctgta ttcgggaccg 420
cgcagtacct tcgatgaaca tgaaagcgca ctgcgcgcat taggtccggg ggcgaccttt 480
gtaggggaag atcacggact ggctgcactg tatgatgcag caatgctgag catcatgtgg 540
ggcctgctga atggtttcct gcatggaacc gcactgctgg gaaccgcggg cgtttcggca 600
acctctgtgg ttccgatgct gggtcaaggt atcgaagcgg tgaccggttg gattgcgcgc 660
tacgcagatc agattgatgc cggtgtctat ccagcagacg acgccacgat tgatacccag 720
cgttatgcaa tgcagcacct ggtcgaggag agccgtgcgg cgggcatcaa tggcgaactt 780
ccggcgtttg tgaccaaact ggccgatcgc gcagtggcag aaggtcgtgg tggtgactct 840
tacgctgcac tgattgaaca gtttcgcaag tcagcttaa                        879

 
<210> 20
<211> 292
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Imine reductase variant from Pseudonocardia spinosispora  
 
<400> 20
Met Ser Asp Ser Pro Val Pro Val Thr Val Leu Gly Leu Gly Leu Met 
1               5                   10                  15      
Gly Gln Ala Leu Ala Gly Ala Phe Leu Ala Ala Gly His Pro Thr Thr 
            20                  25                  30          
Val Trp Asn Arg Ser Ala Ala Lys Ala Asp Glu Leu Val Gly Arg Gly 
        35                  40                  45              
Ala Thr Leu Ala Ala Ser Pro Ala Ala Ala Val Ala Ala Gly Ser Leu 
    50                  55                  60                  
Ile Val Val Cys Val Thr Asp His Arg Ala Val Arg Glu Leu Leu Glu 
65                  70                  75                  80  
Pro Leu Gly Asp Ala Leu Pro Gly Arg Val Leu Val Asn Leu Thr Ser 
                85                  90                  95      
Gly Thr Ser Arg Glu Ala Arg Glu Asn Ala Glu His Thr Ala Ala Gln 
            100                 105                 110         
Gly Ala Thr Tyr Leu Asp Gly Val Ile Leu Thr Ile Pro Ser Gly Ile 
        115                 120                 125             
Gly Thr Pro Glu Ala Thr Ile Leu Tyr Ser Gly Pro Arg Ser Thr Phe 
    130                 135                 140                 
Asp Glu His Glu Ser Ala Leu Arg Ala Leu Gly Pro Gly Ala Thr Phe 
145                 150                 155                 160 
Val Gly Glu Asp His Gly Leu Ala Ala Leu Tyr Asp Ala Ala Met Leu 
                165                 170                 175     
Ser Ile Met Trp Gly Leu Leu Asn Gly Phe Leu His Gly Thr Ala Leu 
            180                 185                 190         
Leu Gly Thr Ala Gly Val Ser Ala Thr Ser Val Val Pro Met Leu Gly 
        195                 200                 205             
Gln Gly Ile Glu Ala Val Thr Gly Trp Ile Ala Arg Tyr Ala Asp Gln 
    210                 215                 220                 
Ile Asp Ala Gly Val Tyr Pro Ala Asp Asp Ala Thr Ile Asp Thr Gln 
225                 230                 235                 240 
Arg Tyr Ala Met Gln His Leu Val Glu Glu Ser Arg Ala Ala Gly Ile 
                245                 250                 255     
Asn Gly Glu Leu Pro Ala Phe Val Thr Lys Leu Ala Asp Arg Ala Val 
            260                 265                 270         
Ala Glu Gly Arg Gly Gly Asp Ser Tyr Ala Ala Leu Ile Glu Gln Phe 
        275                 280                 285             
Arg Lys Ser Ala                                                 
    290                                                         

 
<210> 21
<211> 879
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Imine reductase variant from Pseudonocardia spinosispora  
 
<400> 21
atgagtgatt cccccgtacc tgtgactgtt ctgggtcttg gtctgatggg tcaggcctta 60
gcgggagcgt ttctggcagc cggtcaccct acgacggttt ggaatcgcag cgcagctaaa 120
gctgatgaac tggttggtcg cggtgcaacc ctggccgcgt ctcccgcggc tgccgtcgca 180
gcgggtagtc tgatcgttgt ttgcgtcaca gatcatcgtg cggtgcgcga actgctggaa 240
ccacttggcg atgcactgcc aggtcgcgtt ctggtcaacc tgacgagcgg tacgtcgcgt 300
gaagcccgtg agaacgcaga acatactgcg gctcagggag ccacctacct ggatggtgtg 360
attctgacca ttccaagcgg cattggcacc ccagaagcta ccattctgta ttcgggaccg 420
cgcagtacct tcgatgaaca tgaaagcgca ctgcgcgcat taggtccggg ggcgaccttt 480
gtaggggaag atcacggact ggctgcactg tatgatgcag caatgctgag cgtcatgtgg 540
ggcctgctga atggtttcct gcatggaacc gcactgctgg gaaccgcggg cgtttcggca 600
acctctgtgg ttccgatgct gggtcaaggt atcgaaacag tgaccggttg gattgcgcgc 660
tacgcagatc agattgatgc cggtgtctat ccagcagacg acgccacgat tgatacccag 720
cgtgctgcaa tgcagcacct ggtcgaggag agccgtgcgg cgggcatcaa tggcgaactt 780
ccggcgtttg tgaccaaact ggccgatcgc gcagtggcag aaggtcgtgg tggtgactct 840
tacgctgcac tgattgaaca gtttcgcaag tcagcttaa                        879

 
<210> 22
<211> 292
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Imine reductase variant from Pseudonocardia spinosispora  
 
<400> 22
Met Ser Asp Ser Pro Val Pro Val Thr Val Leu Gly Leu Gly Leu Met 
1               5                   10                  15      
Gly Gln Ala Leu Ala Gly Ala Phe Leu Ala Ala Gly His Pro Thr Thr 
            20                  25                  30          
Val Trp Asn Arg Ser Ala Ala Lys Ala Asp Glu Leu Val Gly Arg Gly 
        35                  40                  45              
Ala Thr Leu Ala Ala Ser Pro Ala Ala Ala Val Ala Ala Gly Ser Leu 
    50                  55                  60                  
Ile Val Val Cys Val Thr Asp His Arg Ala Val Arg Glu Leu Leu Glu 
65                  70                  75                  80  
Pro Leu Gly Asp Ala Leu Pro Gly Arg Val Leu Val Asn Leu Thr Ser 
                85                  90                  95      
Gly Thr Ser Arg Glu Ala Arg Glu Asn Ala Glu His Thr Ala Ala Gln 
            100                 105                 110         
Gly Ala Thr Tyr Leu Asp Gly Val Ile Leu Thr Ile Pro Ser Gly Ile 
        115                 120                 125             
Gly Thr Pro Glu Ala Thr Ile Leu Tyr Ser Gly Pro Arg Ser Thr Phe 
    130                 135                 140                 
Asp Glu His Glu Ser Ala Leu Arg Ala Leu Gly Pro Gly Ala Thr Phe 
145                 150                 155                 160 
Val Gly Glu Asp His Gly Leu Ala Ala Leu Tyr Asp Ala Ala Met Leu 
                165                 170                 175     
Ser Val Met Trp Gly Leu Leu Asn Gly Phe Leu His Gly Thr Ala Leu 
            180                 185                 190         
Leu Gly Thr Ala Gly Val Ser Ala Thr Ser Val Val Pro Met Leu Gly 
        195                 200                 205             
Gln Gly Ile Glu Thr Val Thr Gly Trp Ile Ala Arg Tyr Ala Asp Gln 
    210                 215                 220                 
Ile Asp Ala Gly Val Tyr Pro Ala Asp Asp Ala Thr Ile Asp Thr Gln 
225                 230                 235                 240 
Arg Ala Ala Met Gln His Leu Val Glu Glu Ser Arg Ala Ala Gly Ile 
                245                 250                 255     
Asn Gly Glu Leu Pro Ala Phe Val Thr Lys Leu Ala Asp Arg Ala Val 
            260                 265                 270         
Ala Glu Gly Arg Gly Gly Asp Ser Tyr Ala Ala Leu Ile Glu Gln Phe 
        275                 280                 285             
Arg Lys Ser Ala                                                 
    290                                                         

 
<210> 23
<211> 879
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Imine reductase variant from Pseudonocardia spinosispora  
 
<400> 23
atgagtgatt cccccgtacc tgtgactgtt ctgggtcttg gtctgatggg tcaggcctta 60
gcgggagcgt ttctggcagc cggtcaccct acgacggttt ggaatcgcag cgcagctaaa 120
gctgatgaac tggttggtcg cggtgcaacc ctggccgcgt ctcccgcggc tgccgtcgca 180
gcgggtagtc tgatcgttgt ttgcgtcaca gatcatcgtg cggtgcgcga actgctggaa 240
ccacttggcg atgcactgcc aggtcgcgtt ctggtcaacc tgacgagcgg tacgtcgcgt 300
gaagcccgtg agaacgcaga acatactgcg gctcagggag ccacctacct ggatggtgtg 360
attctgacca ttccaagcgg cattggcacc ccagaagcta ccattctgta ttcgggaccg 420
cgcagtacct tcgatgaaca tgaaagcgca ctgcgcgcat taggtccggg ggcgaccttt 480
gtaggggaag atcacggact ggctgcactg tatgatgcag caatgctgag cgtcatgtgg 540
ggcctgctga atggtttcct gcatggaacc gcactgctgg gaaccgcggg cgtttcggca 600
acctctgtgg ttccgatgct gggtcaaggt atcgaagcgg tgaccggtct tattgcgcgc 660
tacgcagatc agattgatgc cggtgtctat ccagcagacg acgccacgat tgatacccag 720
cgtgctgcaa tgcagcacct ggtcgaggag agccgtgcgg cgggcatcaa tggcgaactt 780
ccggcgtttg tgaccaaact ggccgatcgc gcagtggcag aaggtcgtgg tggtgactct 840
tacgctgcac tgattgaaca gtttcgcaag tcagcttaa                        879

 
<210> 24
<211> 292
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Imine reductase variant from Pseudonocardia spinosispora  
 
<400> 24
Met Ser Asp Ser Pro Val Pro Val Thr Val Leu Gly Leu Gly Leu Met 
1               5                   10                  15      
Gly Gln Ala Leu Ala Gly Ala Phe Leu Ala Ala Gly His Pro Thr Thr 
            20                  25                  30          
Val Trp Asn Arg Ser Ala Ala Lys Ala Asp Glu Leu Val Gly Arg Gly 
        35                  40                  45              
Ala Thr Leu Ala Ala Ser Pro Ala Ala Ala Val Ala Ala Gly Ser Leu 
    50                  55                  60                  
Ile Val Val Cys Val Thr Asp His Arg Ala Val Arg Glu Leu Leu Glu 
65                  70                  75                  80  
Pro Leu Gly Asp Ala Leu Pro Gly Arg Val Leu Val Asn Leu Thr Ser 
                85                  90                  95      
Gly Thr Ser Arg Glu Ala Arg Glu Asn Ala Glu His Thr Ala Ala Gln 
            100                 105                 110         
Gly Ala Thr Tyr Leu Asp Gly Val Ile Leu Thr Ile Pro Ser Gly Ile 
        115                 120                 125             
Gly Thr Pro Glu Ala Thr Ile Leu Tyr Ser Gly Pro Arg Ser Thr Phe 
    130                 135                 140                 
Asp Glu His Glu Ser Ala Leu Arg Ala Leu Gly Pro Gly Ala Thr Phe 
145                 150                 155                 160 
Val Gly Glu Asp His Gly Leu Ala Ala Leu Tyr Asp Ala Ala Met Leu 
                165                 170                 175     
Ser Val Met Trp Gly Leu Leu Asn Gly Phe Leu His Gly Thr Ala Leu 
            180                 185                 190         
Leu Gly Thr Ala Gly Val Ser Ala Thr Ser Val Val Pro Met Leu Gly 
        195                 200                 205             
Gln Gly Ile Glu Ala Val Thr Gly Leu Ile Ala Arg Tyr Ala Asp Gln 
    210                 215                 220                 
Ile Asp Ala Gly Val Tyr Pro Ala Asp Asp Ala Thr Ile Asp Thr Gln 
225                 230                 235                 240 
Arg Ala Ala Met Gln His Leu Val Glu Glu Ser Arg Ala Ala Gly Ile 
                245                 250                 255     
Asn Gly Glu Leu Pro Ala Phe Val Thr Lys Leu Ala Asp Arg Ala Val 
            260                 265                 270         
Ala Glu Gly Arg Gly Gly Asp Ser Tyr Ala Ala Leu Ile Glu Gln Phe 
        275                 280                 285             
Arg Lys Ser Ala                                                 
    290                                                         

 
<210> 25
<211> 879
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Imine reductase variant from Pseudonocardia spinosispora  
 
<400> 25
atgagtgatt cccccgtacc tgtgactgtt ctgggtcttg gtctgatggg tcaggcctta 60
gcgggagcgt ttctggcagc cggtcaccct acgacggttt ggaatcgcag cgcagctaaa 120
gctgatgaac tggttggtcg cggtgcaacc ctggccgcgt ctcccgcggc tgccgtcgca 180
gcgggtagtc tgatcgttgt ttgcgtcaca gatcatcgtg cggtgcgcga actgctggaa 240
ccacttggcg atgcactgcc aggtcgcgtt ctggtcaacc tgacgagcgg tacgtcgcgt 300
gaagcccgtg agaacgcaga acatactgcg gctcagggag ccacctacct ggatggtgtg 360
attctgacca ttccaagcgg cattggcacc ccagaagcta ccattctgta ttcgggaccg 420
cgcagtacct tcgatgaaca tgaaagcgca ctgcgcgcat taggtccggg ggcgaccttt 480
gtaggggaag atcacggact ggctgcactg tatgatgcag caatgctgag cgtcatgtgg 540
ggcctgctga atggtttcct gcatggaacc gcactgctgg gaaccgcggg cgtttcggca 600
acctctgtgg ttccgatgct gggtcaaggt atcgaaaggg tgaccggttg gattgcgcgc 660
tacgcagatc agattgatgc cggtgtctat ccagcagacg acgccacgat tgatacccag 720
cgtgctgcaa tgcagcacct ggtcgaggag agccgtgcgg cgggcatcaa tggcgaactt 780
ccggcgtttg tgaccaaact ggccgatcgc gcagtggcag aaggtcgtgg tggtgactct 840
tacgctgcac tgattgaaca gtttcgcaag tcagcttaa                        879

 
<210> 26
<211> 292
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Imine reductase variant from Pseudonocardia spinosispora  
 
<400> 26
Met Ser Asp Ser Pro Val Pro Val Thr Val Leu Gly Leu Gly Leu Met 
1               5                   10                  15      
Gly Gln Ala Leu Ala Gly Ala Phe Leu Ala Ala Gly His Pro Thr Thr 
            20                  25                  30          
Val Trp Asn Arg Ser Ala Ala Lys Ala Asp Glu Leu Val Gly Arg Gly 
        35                  40                  45              
Ala Thr Leu Ala Ala Ser Pro Ala Ala Ala Val Ala Ala Gly Ser Leu 
    50                  55                  60                  
Ile Val Val Cys Val Thr Asp His Arg Ala Val Arg Glu Leu Leu Glu 
65                  70                  75                  80  
Pro Leu Gly Asp Ala Leu Pro Gly Arg Val Leu Val Asn Leu Thr Ser 
                85                  90                  95      
Gly Thr Ser Arg Glu Ala Arg Glu Asn Ala Glu His Thr Ala Ala Gln 
            100                 105                 110         
Gly Ala Thr Tyr Leu Asp Gly Val Ile Leu Thr Ile Pro Ser Gly Ile 
        115                 120                 125             
Gly Thr Pro Glu Ala Thr Ile Leu Tyr Ser Gly Pro Arg Ser Thr Phe 
    130                 135                 140                 
Asp Glu His Glu Ser Ala Leu Arg Ala Leu Gly Pro Gly Ala Thr Phe 
145                 150                 155                 160 
Val Gly Glu Asp His Gly Leu Ala Ala Leu Tyr Asp Ala Ala Met Leu 
                165                 170                 175     
Ser Val Met Trp Gly Leu Leu Asn Gly Phe Leu His Gly Thr Ala Leu 
            180                 185                 190         
Leu Gly Thr Ala Gly Val Ser Ala Thr Ser Val Val Pro Met Leu Gly 
        195                 200                 205             
Gln Gly Ile Glu Arg Val Thr Gly Trp Ile Ala Arg Tyr Ala Asp Gln 
    210                 215                 220                 
Ile Asp Ala Gly Val Tyr Pro Ala Asp Asp Ala Thr Ile Asp Thr Gln 
225                 230                 235                 240 
Arg Ala Ala Met Gln His Leu Val Glu Glu Ser Arg Ala Ala Gly Ile 
                245                 250                 255     
Asn Gly Glu Leu Pro Ala Phe Val Thr Lys Leu Ala Asp Arg Ala Val 
            260                 265                 270         
Ala Glu Gly Arg Gly Gly Asp Ser Tyr Ala Ala Leu Ile Glu Gln Phe 
        275                 280                 285             
Arg Lys Ser Ala                                                 
    290                                                         

 
<210> 27
<211> 879
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Imine reductase variant from Pseudonocardia spinosispora  
 
<400> 27
atgagtgatt cccccgtacc tgtgactgtt ctgggtcttg gtctgatggg tcaggcctta 60
gcgggagcgt ttctggcagc cggtcaccct acgacggttt ggaatcgcag cgcagctaaa 120
gctgatgaac tggttggtcg cggtgcaacc ctggccgcgt ctcccgcggc tgccgtcgca 180
gcgggtagtc tgatcgttgt ttgcgtcaca gatcatcgtg cggtgcgcga actgctggaa 240
ccacttggcg atgcactgcc aggtcgcgtt ctggtcaacc tgacgagcgg tacgtcgcgt 300
gaagcccgtg agaacgcaga acatactgcg gctcagggag ccacctacct ggatggtgtg 360
attctgacca ttccaagcgg cattggcacc ccaagggcta ccattctgta ttcgggaccg 420
cgcagtacct tcgatgaaca tgaaagcgca ctgcgcgcat taggtccggg ggcgaccttt 480
gtaggggaag atcacggact ggctgcactg tatgatgcag caatgctgag cgtcatgtgg 540
ggcctgctga atggtttcct gcatggaacc gcactgctgg gaaccgcggg cgtttcggca 600
acctctgtgg ttccgatgct gggtcaaggt atcgaagcgg tgaccggttg gattgcgcgc 660
tacgcagatc agattgatgc cggtgtctat ccagcagacg acgccacgat tgatacccag 720
cgtgctgcaa tgcagcacct ggtcgaggag agccgtgcgg cgggcatcaa tggcgaactt 780
ccggcgtttg tgaccaaact ggccgatcgc gcagtggcag aaggtcgtgg tggtgactct 840
tacgctgcac tgattgaaca gtttcgcaag tcagcttaa                        879

 
<210> 28
<211> 292
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Imine reductase variant from Pseudonocardia spinosispora  
 
<400> 28
Met Ser Asp Ser Pro Val Pro Val Thr Val Leu Gly Leu Gly Leu Met 
1               5                   10                  15      
Gly Gln Ala Leu Ala Gly Ala Phe Leu Ala Ala Gly His Pro Thr Thr 
            20                  25                  30          
Val Trp Asn Arg Ser Ala Ala Lys Ala Asp Glu Leu Val Gly Arg Gly 
        35                  40                  45              
Ala Thr Leu Ala Ala Ser Pro Ala Ala Ala Val Ala Ala Gly Ser Leu 
    50                  55                  60                  
Ile Val Val Cys Val Thr Asp His Arg Ala Val Arg Glu Leu Leu Glu 
65                  70                  75                  80  
Pro Leu Gly Asp Ala Leu Pro Gly Arg Val Leu Val Asn Leu Thr Ser 
                85                  90                  95      
Gly Thr Ser Arg Glu Ala Arg Glu Asn Ala Glu His Thr Ala Ala Gln 
            100                 105                 110         
Gly Ala Thr Tyr Leu Asp Gly Val Ile Leu Thr Ile Pro Ser Gly Ile 
        115                 120                 125             
Gly Thr Pro Arg Ala Thr Ile Leu Tyr Ser Gly Pro Arg Ser Thr Phe 
    130                 135                 140                 
Asp Glu His Glu Ser Ala Leu Arg Ala Leu Gly Pro Gly Ala Thr Phe 
145                 150                 155                 160 
Val Gly Glu Asp His Gly Leu Ala Ala Leu Tyr Asp Ala Ala Met Leu 
                165                 170                 175     
Ser Val Met Trp Gly Leu Leu Asn Gly Phe Leu His Gly Thr Ala Leu 
            180                 185                 190         
Leu Gly Thr Ala Gly Val Ser Ala Thr Ser Val Val Pro Met Leu Gly 
        195                 200                 205             
Gln Gly Ile Glu Ala Val Thr Gly Trp Ile Ala Arg Tyr Ala Asp Gln 
    210                 215                 220                 
Ile Asp Ala Gly Val Tyr Pro Ala Asp Asp Ala Thr Ile Asp Thr Gln 
225                 230                 235                 240 
Arg Ala Ala Met Gln His Leu Val Glu Glu Ser Arg Ala Ala Gly Ile 
                245                 250                 255     
Asn Gly Glu Leu Pro Ala Phe Val Thr Lys Leu Ala Asp Arg Ala Val 
            260                 265                 270         
Ala Glu Gly Arg Gly Gly Asp Ser Tyr Ala Ala Leu Ile Glu Gln Phe 
        275                 280                 285             
Arg Lys Ser Ala                                                 
    290                                                         

 
<210> 29
<211> 879
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Imine reductase variant from Pseudonocardia spinosispora  
 
<400> 29
atgagtgatt cccccgtacc tgtgactgtt ctgggtcttg gtctgatggg tcaggcctta 60
gcgggagcgt ttctggcagc cggtcaccct acgacggttt ggaatcgcag cgcagctaaa 120
gctgatgaac tggttggtcg cggtgcaacc ctggccgcgt ctcccgcggc tgccgtcgca 180
gcgggtagtc tgatcgttgt ttgcgtcaca gatcatcgtg cggtgcgcga actgctggaa 240
ccacttggcg atgcactgtc aggtcgcgtt ctggtcaacc tgacgagcgg tacgtcgcgt 300
gaagcccgtg agaacgcaga acatactgcg gctcagggag ccacctacct ggatggtgtg 360
attctgacca ttccaagcgg cattggcacc ccagaagcta ccattctgta ttcgggaccg 420
cgcagtacct tcgatgaaca tgaaagcgca ctgcgcgcat taggtccggg ggcgaccttt 480
gtaggggaag atcacggact ggctgcactg tatgatgcag caggtctgag cgtcatgtgg 540
ggcctgctga atggtttcct gcatggaacc gcactgctgg gaaccgcggg cgtttcggca 600
acctctgtgg ttccgatgct gggtcaaggt atcgaagcgg tgaccggttg gattgcgcgc 660
tacgcagatc agattgatgc cggtgtctat ccagcagacg acgccacgat tgatacccag 720
cgtgctgcaa tgcagcacct ggtcgaggag agccgtgcgg cgggcatcaa tggcgaactt 780
ccggcgtttg tgaccaaact ggccgatcgc gcagtggcag aaggtcgtgg tggtgactct 840
tacgctgcac tgattgaaca gtttcgcaag tcagcttaa                        879

 
<210> 30
<211> 292
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Imine reductase variant from Pseudonocardia spinosispora  
 
<400> 30
Met Ser Asp Ser Pro Val Pro Val Thr Val Leu Gly Leu Gly Leu Met 
1               5                   10                  15      
Gly Gln Ala Leu Ala Gly Ala Phe Leu Ala Ala Gly His Pro Thr Thr 
            20                  25                  30          
Val Trp Asn Arg Ser Ala Ala Lys Ala Asp Glu Leu Val Gly Arg Gly 
        35                  40                  45              
Ala Thr Leu Ala Ala Ser Pro Ala Ala Ala Val Ala Ala Gly Ser Leu 
    50                  55                  60                  
Ile Val Val Cys Val Thr Asp His Arg Ala Val Arg Glu Leu Leu Glu 
65                  70                  75                  80  
Pro Leu Gly Asp Ala Leu Ser Gly Arg Val Leu Val Asn Leu Thr Ser 
                85                  90                  95      
Gly Thr Ser Arg Glu Ala Arg Glu Asn Ala Glu His Thr Ala Ala Gln 
            100                 105                 110         
Gly Ala Thr Tyr Leu Asp Gly Val Ile Leu Thr Ile Pro Ser Gly Ile 
        115                 120                 125             
Gly Thr Pro Glu Ala Thr Ile Leu Tyr Ser Gly Pro Arg Ser Thr Phe 
    130                 135                 140                 
Asp Glu His Glu Ser Ala Leu Arg Ala Leu Gly Pro Gly Ala Thr Phe 
145                 150                 155                 160 
Val Gly Glu Asp His Gly Leu Ala Ala Leu Tyr Asp Ala Ala Gly Leu 
                165                 170                 175     
Ser Val Met Trp Gly Leu Leu Asn Gly Phe Leu His Gly Thr Ala Leu 
            180                 185                 190         
Leu Gly Thr Ala Gly Val Ser Ala Thr Ser Val Val Pro Met Leu Gly 
        195                 200                 205             
Gln Gly Ile Glu Ala Val Thr Gly Trp Ile Ala Arg Tyr Ala Asp Gln 
    210                 215                 220                 
Ile Asp Ala Gly Val Tyr Pro Ala Asp Asp Ala Thr Ile Asp Thr Gln 
225                 230                 235                 240 
Arg Ala Ala Met Gln His Leu Val Glu Glu Ser Arg Ala Ala Gly Ile 
                245                 250                 255     
Asn Gly Glu Leu Pro Ala Phe Val Thr Lys Leu Ala Asp Arg Ala Val 
            260                 265                 270         
Ala Glu Gly Arg Gly Gly Asp Ser Tyr Ala Ala Leu Ile Glu Gln Phe 
        275                 280                 285             
Arg Lys Ser Ala                                                 
    290                                                         

 
<210> 31
<211> 879
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Imine reductase variant from Pseudonocardia spinosispora  
 
<400> 31
atgagtgatt cccccgtacc tgtgactgtt ctgggtcttg gtctgatggg tcaggcctta 60
gcgggagcgt ttctggcagc cggtcaccct acgacggttt ggaatcgcag cgcagctaaa 120
gctgatgaac tggttggtcg cggtgcaacc ctggccgcgt ctcccgcggc tgccgtcgca 180
gcgggtagtc tgatcgttgt ttgcgtcaca gatcatcgtg cggtgcgcga actgctggaa 240
ccacttggcg atgcactgcc aggtcgcgtt ctggtcaacc tgacgagcgg tacgtcgcgt 300
gaagcccgtg agaacgcaga acatactgcg gctcagggag ccacctacct ggatggtgtg 360
attctgacca ttccaagcgg cattggcacc ccagaagcta ccattctgta ttcgggaccg 420
cgcagtacct tcgatgaaca tgaaagcgca ctgcgcgcat taggtccggg ggcgaccttt 480
gtaggggaag atcacggact ggctgcactg tatgatgcag caatgagtag cgtcatgtgg 540
ggcctgctga atggtttcct gcatggaacc gcactgctgg gaaccgcggg cgtttcggca 600
acctctgtgg ttccgatgct gggtcaaggt atcgaagcgg tgaccggttg gattgcgcgc 660
tacgcagatc agattgatgc cggtgtctat ccagcagacg acgccacgat tgatacccag 720
cgtgctgcaa tgcagcacct ggtcgaggag agccgtgcgg cgggcatcaa tggcgaactt 780
ccggcgtttg tgaccaaact ggccgatcgc gcagtggcag aaggtcgtgg tggtgactct 840
tacgctgcac tgattgaaca gtttcgcaag tcagcttaa                        879

 
<210> 32
<211> 292
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Imine reductase variant from Pseudonocardia spinosispora  
 
<400> 32
Met Ser Asp Ser Pro Val Pro Val Thr Val Leu Gly Leu Gly Leu Met 
1               5                   10                  15      
Gly Gln Ala Leu Ala Gly Ala Phe Leu Ala Ala Gly His Pro Thr Thr 
            20                  25                  30          
Val Trp Asn Arg Ser Ala Ala Lys Ala Asp Glu Leu Val Gly Arg Gly 
        35                  40                  45              
Ala Thr Leu Ala Ala Ser Pro Ala Ala Ala Val Ala Ala Gly Ser Leu 
    50                  55                  60                  
Ile Val Val Cys Val Thr Asp His Arg Ala Val Arg Glu Leu Leu Glu 
65                  70                  75                  80  
Pro Leu Gly Asp Ala Leu Pro Gly Arg Val Leu Val Asn Leu Thr Ser 
                85                  90                  95      
Gly Thr Ser Arg Glu Ala Arg Glu Asn Ala Glu His Thr Ala Ala Gln 
            100                 105                 110         
Gly Ala Thr Tyr Leu Asp Gly Val Ile Leu Thr Ile Pro Ser Gly Ile 
        115                 120                 125             
Gly Thr Pro Glu Ala Thr Ile Leu Tyr Ser Gly Pro Arg Ser Thr Phe 
    130                 135                 140                 
Asp Glu His Glu Ser Ala Leu Arg Ala Leu Gly Pro Gly Ala Thr Phe 
145                 150                 155                 160 
Val Gly Glu Asp His Gly Leu Ala Ala Leu Tyr Asp Ala Ala Met Ser 
                165                 170                 175     
Ser Val Met Trp Gly Leu Leu Asn Gly Phe Leu His Gly Thr Ala Leu 
            180                 185                 190         
Leu Gly Thr Ala Gly Val Ser Ala Thr Ser Val Val Pro Met Leu Gly 
        195                 200                 205             
Gln Gly Ile Glu Ala Val Thr Gly Trp Ile Ala Arg Tyr Ala Asp Gln 
    210                 215                 220                 
Ile Asp Ala Gly Val Tyr Pro Ala Asp Asp Ala Thr Ile Asp Thr Gln 
225                 230                 235                 240 
Arg Ala Ala Met Gln His Leu Val Glu Glu Ser Arg Ala Ala Gly Ile 
                245                 250                 255     
Asn Gly Glu Leu Pro Ala Phe Val Thr Lys Leu Ala Asp Arg Ala Val 
            260                 265                 270         
Ala Glu Gly Arg Gly Gly Asp Ser Tyr Ala Ala Leu Ile Glu Gln Phe 
        275                 280                 285             
Arg Lys Ser Ala                                                 
    290                                                         

 
<210> 33
<211> 879
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Imine reductase variant from Pseudonocardia spinosispora  
 
<400> 33
atgagtgatt cccccgtacc tgtgactgtt ctgggtcttg gtctgatggg tcaggcctta 60
gcgggagcgt ttctggcagc cggtcaccct acgacggttt ggaatcgcag cgcagctaaa 120
gctgatgaac tggttggtcg cggtgcaacc ctggccgcgt ctcccgcggc tgccgtcgca 180
gcgggtagtc tgatcgttgt ttgcgtcaca gatcatcgtg cggtgcgcga actgctggaa 240
ccacttggcg atgcactgcc aggtcgcgtt ctggtcaacc tgacgagcgg tacgtcgcgt 300
gaagcccgtg agaacgcaga acatactgcg gctcagggag ccacctacct ggatggtgtg 360
attctgacca ttccaagcgg cattggcacc ccagaagcta ccattctgta ttcgggaccg 420
cgcagtacct tcgatgaaca tgaaagcgca ctgcgcgcat taggtccggg ggcgaccttt 480
gtaggggaag atcacggact ggctgcactg tatgatgcag caggtctgag cgtcatgtgg 540
ggcctgctga atggtttcct gcatggaacc gcactgctgg gaaccgcggg cgtttcggca 600
acctctgtgg ttccgatgct gggtcaaggt atcgaagcgg tgaccggttg gattgcgcgc 660
tacgcagatc agattgatgc cggtgtctat ccagcagacg acgccacgat tgatacccag 720
cgtgctgcaa tgcagcacct ggtcgaggag agccgtgcgg cgggcatcaa tggcgaactt 780
ccggcgtttg tgaccaaact ggccgatcgc gcagtggcag aaggtcgtgg tggtgactct 840
tacgctgcac tgattgaaca gtttcgcaag tcagcttaa                        879

 
<210> 34
<211> 292
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Imine reductase variant from Pseudonocardia spinosispora  
 
<400> 34
Met Ser Asp Ser Pro Val Pro Val Thr Val Leu Gly Leu Gly Leu Met 
1               5                   10                  15      
Gly Gln Ala Leu Ala Gly Ala Phe Leu Ala Ala Gly His Pro Thr Thr 
            20                  25                  30          
Val Trp Asn Arg Ser Ala Ala Lys Ala Asp Glu Leu Val Gly Arg Gly 
        35                  40                  45              
Ala Thr Leu Ala Ala Ser Pro Ala Ala Ala Val Ala Ala Gly Ser Leu 
    50                  55                  60                  
Ile Val Val Cys Val Thr Asp His Arg Ala Val Arg Glu Leu Leu Glu 
65                  70                  75                  80  
Pro Leu Gly Asp Ala Leu Pro Gly Arg Val Leu Val Asn Leu Thr Ser 
                85                  90                  95      
Gly Thr Ser Arg Glu Ala Arg Glu Asn Ala Glu His Thr Ala Ala Gln 
            100                 105                 110         
Gly Ala Thr Tyr Leu Asp Gly Val Ile Leu Thr Ile Pro Ser Gly Ile 
        115                 120                 125             
Gly Thr Pro Glu Ala Thr Ile Leu Tyr Ser Gly Pro Arg Ser Thr Phe 
    130                 135                 140                 
Asp Glu His Glu Ser Ala Leu Arg Ala Leu Gly Pro Gly Ala Thr Phe 
145                 150                 155                 160 
Val Gly Glu Asp His Gly Leu Ala Ala Leu Tyr Asp Ala Ala Gly Leu 
                165                 170                 175     
Ser Val Met Trp Gly Leu Leu Asn Gly Phe Leu His Gly Thr Ala Leu 
            180                 185                 190         
Leu Gly Thr Ala Gly Val Ser Ala Thr Ser Val Val Pro Met Leu Gly 
        195                 200                 205             
Gln Gly Ile Glu Ala Val Thr Gly Trp Ile Ala Arg Tyr Ala Asp Gln 
    210                 215                 220                 
Ile Asp Ala Gly Val Tyr Pro Ala Asp Asp Ala Thr Ile Asp Thr Gln 
225                 230                 235                 240 
Arg Ala Ala Met Gln His Leu Val Glu Glu Ser Arg Ala Ala Gly Ile 
                245                 250                 255     
Asn Gly Glu Leu Pro Ala Phe Val Thr Lys Leu Ala Asp Arg Ala Val 
            260                 265                 270         
Ala Glu Gly Arg Gly Gly Asp Ser Tyr Ala Ala Leu Ile Glu Gln Phe 
        275                 280                 285             
Arg Lys Ser Ala                                                 
    290                                                         

 
<210> 35
<211> 879
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Imine reductase variant from Pseudonocardia spinosispora  
 
<400> 35
atgagtgatt cccccgtacc tgtgactgtt ctgggtcttg gtctgatggg tcaggcctta 60
gcgggagcgt ttctggcagc cggtcaccct acgacggttt ggaatcgcag cgcagctaaa 120
gctgatgaac tggttggtcg cggtgcaacc ctggccgcgt ctcccgcggc tgccgtcgca 180
gcgggtagtc tgatcgttgt ttgcgtcaca gatcatcgtg cggtgcgcga actgctggaa 240
ccacttggcg atgcactgcc aggtcgcgtt ctggtcaacc tgacgagcgg tacgtcgcgt 300
gaagcccgtg agaacgcaga acatactgcg gctcagggag ccacctacct ggatggtgtg 360
attctgacca ttccaagcgg cattggcacc ccagaagcta ccattctgta ttcgggaccg 420
cgcagtacct tcgatgaaca tgaaagcgca ctgcgcgcat taggtccggg ggcgaccttt 480
gtaggggaag atcacggact ggctgcactg tatgatgcag caatgctgag cgtcatgtgg 540
ggcctgctga atggtttcct gcatggaacc gcactgctgg gaaccgcggg cgtttcggca 600
acctctgtgg ttccgatgct gggtcaaggt atcgaagttg tgaccggttg gattgcgcgc 660
tacgcagatc agattgatgc cggtgtctat ccagcagacg acgccacgat tgatacccag 720
cgtgctgcaa tgcagcacct ggtcgaggag agccgtgcgg cgggcatcaa tggcgaactt 780
ccggcgtttg tgaccaaact ggccgatcgc gcagtggcag aaggtcgtgg tggtgactct 840
tacgctgcac tgattgaaca gtttcgcaag tcagcttaa                        879

 
<210> 36
<211> 292
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Imine reductase variant from Pseudonocardia spinosispora  
 
<400> 36
Met Ser Asp Ser Pro Val Pro Val Thr Val Leu Gly Leu Gly Leu Met 
1               5                   10                  15      
Gly Gln Ala Leu Ala Gly Ala Phe Leu Ala Ala Gly His Pro Thr Thr 
            20                  25                  30          
Val Trp Asn Arg Ser Ala Ala Lys Ala Asp Glu Leu Val Gly Arg Gly 
        35                  40                  45              
Ala Thr Leu Ala Ala Ser Pro Ala Ala Ala Val Ala Ala Gly Ser Leu 
    50                  55                  60                  
Ile Val Val Cys Val Thr Asp His Arg Ala Val Arg Glu Leu Leu Glu 
65                  70                  75                  80  
Pro Leu Gly Asp Ala Leu Pro Gly Arg Val Leu Val Asn Leu Thr Ser 
                85                  90                  95      
Gly Thr Ser Arg Glu Ala Arg Glu Asn Ala Glu His Thr Ala Ala Gln 
            100                 105                 110         
Gly Ala Thr Tyr Leu Asp Gly Val Ile Leu Thr Ile Pro Ser Gly Ile 
        115                 120                 125             
Gly Thr Pro Glu Ala Thr Ile Leu Tyr Ser Gly Pro Arg Ser Thr Phe 
    130                 135                 140                 
Asp Glu His Glu Ser Ala Leu Arg Ala Leu Gly Pro Gly Ala Thr Phe 
145                 150                 155                 160 
Val Gly Glu Asp His Gly Leu Ala Ala Leu Tyr Asp Ala Ala Met Leu 
                165                 170                 175     
Ser Val Met Trp Gly Leu Leu Asn Gly Phe Leu His Gly Thr Ala Leu 
            180                 185                 190         
Leu Gly Thr Ala Gly Val Ser Ala Thr Ser Val Val Pro Met Leu Gly 
        195                 200                 205             
Gln Gly Ile Glu Val Val Thr Gly Trp Ile Ala Arg Tyr Ala Asp Gln 
    210                 215                 220                 
Ile Asp Ala Gly Val Tyr Pro Ala Asp Asp Ala Thr Ile Asp Thr Gln 
225                 230                 235                 240 
Arg Ala Ala Met Gln His Leu Val Glu Glu Ser Arg Ala Ala Gly Ile 
                245                 250                 255     
Asn Gly Glu Leu Pro Ala Phe Val Thr Lys Leu Ala Asp Arg Ala Val 
            260                 265                 270         
Ala Glu Gly Arg Gly Gly Asp Ser Tyr Ala Ala Leu Ile Glu Gln Phe 
        275                 280                 285             
Arg Lys Ser Ala                                                 
    290                                                         

 
<210> 37
<211> 879
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Imine reductase variant from Pseudonocardia spinosispora  
 
<400> 37
atgagtgatt cccccgtacc tgtgactgtt ctgggtcttg gtctgatggg tcaggcctta 60
gcgggagcgt ttctggcagc cggtcaccct acgacggttt ggaatcgcag cgcagctaaa 120
gctgatgaac tggttggtcg cggtgcaacc ctggccgcgt ctcccgcggc tgccgtcgca 180
gcgggtagtc tgatcgttgt ttgcgtcaca gatcatcgtg cggtgcgcga actgctggaa 240
ccacttggcg atgcactgcc aggtcgcgtt ctggtcaacc tgacgagcgg tacgtcgcgt 300
gaagcccgtg agaacgcaga acatactgcg gctcagggag ccacctacct ggatggtgtg 360
attctgacca ttccaagcgg cattggcacc ccagaagcta ccattctgta ttcgggaccg 420
cgcagtacct tcgatgaaca tgaaagcgca ctgcgcgcat taggtccggg ggcgaccttt 480
gtaggggaag atcacggact ggctgcactg tatgatattg caatgctgag cgtcatgtgg 540
ggcctgctga atggtttcct gcatggaacc gcactgctgg gaaccgcggg cgtttcggca 600
acctctgtgg ttccgatgct gggtcaaggt atcgaagcgg tgaccggttg gattgcgcgc 660
tacgcagatc agattgatgc cggtgtctat ccagcagacg acgccacgat tgatacccag 720
cgtgctgcaa tgcagcacct ggtcgaggag agccgtgcgg cgggcatcaa tggcgaactt 780
ccggcgtttg tgaccaaact ggccgatcgc gcagtggcag aaggtcgtgg tggtgactct 840
tacgctgcac tgattgaaca gtttcgcaag tcagcttaa                        879

 
<210> 38
<211> 292
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Imine reductase variant from Pseudonocardia spinosispora  
 
<400> 38
Met Ser Asp Ser Pro Val Pro Val Thr Val Leu Gly Leu Gly Leu Met 
1               5                   10                  15      
Gly Gln Ala Leu Ala Gly Ala Phe Leu Ala Ala Gly His Pro Thr Thr 
            20                  25                  30          
Val Trp Asn Arg Ser Ala Ala Lys Ala Asp Glu Leu Val Gly Arg Gly 
        35                  40                  45              
Ala Thr Leu Ala Ala Ser Pro Ala Ala Ala Val Ala Ala Gly Ser Leu 
    50                  55                  60                  
Ile Val Val Cys Val Thr Asp His Arg Ala Val Arg Glu Leu Leu Glu 
65                  70                  75                  80  
Pro Leu Gly Asp Ala Leu Pro Gly Arg Val Leu Val Asn Leu Thr Ser 
                85                  90                  95      
Gly Thr Ser Arg Glu Ala Arg Glu Asn Ala Glu His Thr Ala Ala Gln 
            100                 105                 110         
Gly Ala Thr Tyr Leu Asp Gly Val Ile Leu Thr Ile Pro Ser Gly Ile 
        115                 120                 125             
Gly Thr Pro Glu Ala Thr Ile Leu Tyr Ser Gly Pro Arg Ser Thr Phe 
    130                 135                 140                 
Asp Glu His Glu Ser Ala Leu Arg Ala Leu Gly Pro Gly Ala Thr Phe 
145                 150                 155                 160 
Val Gly Glu Asp His Gly Leu Ala Ala Leu Tyr Asp Ile Ala Met Leu 
                165                 170                 175     
Ser Val Met Trp Gly Leu Leu Asn Gly Phe Leu His Gly Thr Ala Leu 
            180                 185                 190         
Leu Gly Thr Ala Gly Val Ser Ala Thr Ser Val Val Pro Met Leu Gly 
        195                 200                 205             
Gln Gly Ile Glu Ala Val Thr Gly Trp Ile Ala Arg Tyr Ala Asp Gln 
    210                 215                 220                 
Ile Asp Ala Gly Val Tyr Pro Ala Asp Asp Ala Thr Ile Asp Thr Gln 
225                 230                 235                 240 
Arg Ala Ala Met Gln His Leu Val Glu Glu Ser Arg Ala Ala Gly Ile 
                245                 250                 255     
Asn Gly Glu Leu Pro Ala Phe Val Thr Lys Leu Ala Asp Arg Ala Val 
            260                 265                 270         
Ala Glu Gly Arg Gly Gly Asp Ser Tyr Ala Ala Leu Ile Glu Gln Phe 
        275                 280                 285             
Arg Lys Ser Ala                                                 
    290                                                         

 
<210> 39
<211> 879
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Imine reductase variant from Pseudonocardia spinosispora  
 
<400> 39
atgagtgatt cccccgtacc tgtgactgtt ctgggtcttg gtctgatggg tcaggcctta 60
gcgggagcgt ttctggcagc cggtcaccct acgacggttt ggaatcgcag cgcagctaaa 120
gctgatgaac tggttggtcg cggtgcaacc ctggccgcgt ctcccgcggc tgccgtcgca 180
gcgggtagtc tgatcgttgt ttgcgtcaca gatcatcgtg cggtgcgcga actgctggaa 240
ccacttggcg atgcactgcc aggtcgcgtt ctggtcaacc tgacgagcgg tacgtcgcgt 300
gaagcccgtg agaacgcaga acatactgcg gctcagggag ccacctacct ggatggtgtg 360
attgaaacca ttccaagcgg cattggcacc ccagaagcta ccattctgta ttcgggaccg 420
cgcagtacct tcgatgaaca tgaaagcgca ctgcgcgcat taggtccggg ggcgaccttt 480
gtaggggaag atcacggact ggctgcactg tatgatgcag caatgctgag cgtcatgtgg 540
ggcctgctga atggtttcct gcatggaacc gcactgctgg gaaccgcggg cgtttcggca 600
acctctgtgg ttccgatgct gggtcaaggt atcgaagcgg tgaccggttg gattgcgcgc 660
tacgcagatc agattgatgc cggtgtctat ccagcagacg acgccacgat tgatacccag 720
cgtgctgcaa tgcagcacct ggtcgaggag agccgtgcgg cgggcatcaa tggcgaactt 780
ccggcgtttg tgaccaaact ggccgatcgc gcagtggcag aaggtcgtgg tggtgactct 840
tacgctgcac tgattgaaca gtttcgcaag tcagcttaa                        879

 
<210> 40
<211> 292
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Imine reductase variant from Pseudonocardia spinosispora  
 
<400> 40
Met Ser Asp Ser Pro Val Pro Val Thr Val Leu Gly Leu Gly Leu Met 
1               5                   10                  15      
Gly Gln Ala Leu Ala Gly Ala Phe Leu Ala Ala Gly His Pro Thr Thr 
            20                  25                  30          
Val Trp Asn Arg Ser Ala Ala Lys Ala Asp Glu Leu Val Gly Arg Gly 
        35                  40                  45              
Ala Thr Leu Ala Ala Ser Pro Ala Ala Ala Val Ala Ala Gly Ser Leu 
    50                  55                  60                  
Ile Val Val Cys Val Thr Asp His Arg Ala Val Arg Glu Leu Leu Glu 
65                  70                  75                  80  
Pro Leu Gly Asp Ala Leu Pro Gly Arg Val Leu Val Asn Leu Thr Ser 
                85                  90                  95      
Gly Thr Ser Arg Glu Ala Arg Glu Asn Ala Glu His Thr Ala Ala Gln 
            100                 105                 110         
Gly Ala Thr Tyr Leu Asp Gly Val Ile Glu Thr Ile Pro Ser Gly Ile 
        115                 120                 125             
Gly Thr Pro Glu Ala Thr Ile Leu Tyr Ser Gly Pro Arg Ser Thr Phe 
    130                 135                 140                 
Asp Glu His Glu Ser Ala Leu Arg Ala Leu Gly Pro Gly Ala Thr Phe 
145                 150                 155                 160 
Val Gly Glu Asp His Gly Leu Ala Ala Leu Tyr Asp Ala Ala Met Leu 
                165                 170                 175     
Ser Val Met Trp Gly Leu Leu Asn Gly Phe Leu His Gly Thr Ala Leu 
            180                 185                 190         
Leu Gly Thr Ala Gly Val Ser Ala Thr Ser Val Val Pro Met Leu Gly 
        195                 200                 205             
Gln Gly Ile Glu Ala Val Thr Gly Trp Ile Ala Arg Tyr Ala Asp Gln 
    210                 215                 220                 
Ile Asp Ala Gly Val Tyr Pro Ala Asp Asp Ala Thr Ile Asp Thr Gln 
225                 230                 235                 240 
Arg Ala Ala Met Gln His Leu Val Glu Glu Ser Arg Ala Ala Gly Ile 
                245                 250                 255     
Asn Gly Glu Leu Pro Ala Phe Val Thr Lys Leu Ala Asp Arg Ala Val 
            260                 265                 270         
Ala Glu Gly Arg Gly Gly Asp Ser Tyr Ala Ala Leu Ile Glu Gln Phe 
        275                 280                 285             
Arg Lys Ser Ala                                                 
    290                                                         

 
<210> 41
<211> 879
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Imine reductase variant from Pseudonocardia spinosispora  
 
<400> 41
atgagtgatt cccccgtacc tgtgactgtt ctgggtcttg gtctgatggg tcaggcctta 60
gcgggagcgt ttctggcagc cggtcaccct acgacggttt ggaatcgcag cgcagctaaa 120
gctgatgaac tggttggtcg cggtgcaacc ctggccgcgt ctcccgcggc tgccgtcgca 180
gcgggtagtc tgatcgttgt ttgcgtcaca gatcatcgtg cggtgcgcga actgctggaa 240
ccacttggcg atgcactgcc aggtcgcgtt ctggtcaacc tgacgagcgg tacgtcgcgt 300
gaagcccgtg agaacgcaga acatactgcg gctcagggag ccacctacct ggatggtgtg 360
attctgacca ttccaagcgg cattggcacc ccagaagcta ccattctgta ttcgggaccg 420
cgcagtacct tcgatgaaca tgaaagcgca ctgcgcgcat taggtccggg ggcgaccttt 480
gtaggggaag atcacggact ggctgcactg tatgatgcag caatgctgag cgtcatgtgg 540
ggcctgctga atggtttcct gcatggaacc gcactgctgg gaaccgcggg cgtttcggca 600
acctctgtgg ttccgatgct gggtcaaggt atcgaaccag tgaccggttg gattgcgcgc 660
tacgcagatc agattgatgc cggtgtctat ccagcagacg acgccacgat tgatacccag 720
cgtgctgcaa tgcagcacct ggtcgaggag agccgtgcgg cgggcatcaa tggcgaactt 780
ccggcgtttg tgaccaaact ggccgatcgc gcagtggcag aaggtcgtgg tggtgactct 840
tacgctgcac tgattgaaca gtttcgcaag tcagcttaa                        879

 
<210> 42
<211> 292
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Imine reductase variant from Pseudonocardia spinosispora  
 
<400> 42
Met Ser Asp Ser Pro Val Pro Val Thr Val Leu Gly Leu Gly Leu Met 
1               5                   10                  15      
Gly Gln Ala Leu Ala Gly Ala Phe Leu Ala Ala Gly His Pro Thr Thr 
            20                  25                  30          
Val Trp Asn Arg Ser Ala Ala Lys Ala Asp Glu Leu Val Gly Arg Gly 
        35                  40                  45              
Ala Thr Leu Ala Ala Ser Pro Ala Ala Ala Val Ala Ala Gly Ser Leu 
    50                  55                  60                  
Ile Val Val Cys Val Thr Asp His Arg Ala Val Arg Glu Leu Leu Glu 
65                  70                  75                  80  
Pro Leu Gly Asp Ala Leu Pro Gly Arg Val Leu Val Asn Leu Thr Ser 
                85                  90                  95      
Gly Thr Ser Arg Glu Ala Arg Glu Asn Ala Glu His Thr Ala Ala Gln 
            100                 105                 110         
Gly Ala Thr Tyr Leu Asp Gly Val Ile Leu Thr Ile Pro Ser Gly Ile 
        115                 120                 125             
Gly Thr Pro Glu Ala Thr Ile Leu Tyr Ser Gly Pro Arg Ser Thr Phe 
    130                 135                 140                 
Asp Glu His Glu Ser Ala Leu Arg Ala Leu Gly Pro Gly Ala Thr Phe 
145                 150                 155                 160 
Val Gly Glu Asp His Gly Leu Ala Ala Leu Tyr Asp Ala Ala Met Leu 
                165                 170                 175     
Ser Val Met Trp Gly Leu Leu Asn Gly Phe Leu His Gly Thr Ala Leu 
            180                 185                 190         
Leu Gly Thr Ala Gly Val Ser Ala Thr Ser Val Val Pro Met Leu Gly 
        195                 200                 205             
Gln Gly Ile Glu Pro Val Thr Gly Trp Ile Ala Arg Tyr Ala Asp Gln 
    210                 215                 220                 
Ile Asp Ala Gly Val Tyr Pro Ala Asp Asp Ala Thr Ile Asp Thr Gln 
225                 230                 235                 240 
Arg Ala Ala Met Gln His Leu Val Glu Glu Ser Arg Ala Ala Gly Ile 
                245                 250                 255     
Asn Gly Glu Leu Pro Ala Phe Val Thr Lys Leu Ala Asp Arg Ala Val 
            260                 265                 270         
Ala Glu Gly Arg Gly Gly Asp Ser Tyr Ala Ala Leu Ile Glu Gln Phe 
        275                 280                 285             
Arg Lys Ser Ala                                                 
    290                                                         

 
<210> 43
<211> 879
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Imine reductase variant from Pseudonocardia spinosispora  
 
<400> 43
atgagtgatt cccccgtacc tgtgactgtt ctgggtcttg gtctgatggg tcaggcctta 60
gcgggagcgt ttctggcagc cggtcaccct acgacggttt ggaatcgcag cgcagctaaa 120
gctgatgaac tggttggtcg cggtgcaacc ctggccgcgt ctcccgcggc tgccgtcgca 180
gcgggtagtc tgatcgttgt ttgcgtcaca gatcatcgtg cggtgcgcga actgctggaa 240
ccacttggcg atgcactgcc aggtcgcgtt ctggtcaacc tgacgagcgg tacgtcgcgt 300
gaagcccgtg agaacgcaga acatactgcg gctcagggag ccacctacct ggatggtgtg 360
attctgacca ttccaagcgg cattggcacc ccagaagcta ccattctgta ttcgggaccg 420
cgcagtacct tcgatgaaca tgaaagcgca ctgcgcgcat taggtccggg ggcgaccttt 480
gtaggggaag atcacggact ggctgcactg tatgatgcag caatgctgag cgtcatgtgg 540
ggcctgctga atggtttcct gcatggaacc gcactgctgg gaaccgcggg cgtttcggca 600
acctctgtgg ttccgatgct gggtcaaggt atcgaagcgg tgaccggtat tattgcgcgc 660
tacgcagatc agattgatgc cggtgtctat ccagcagacg acgccacgat tgatacccag 720
cgtgctgcaa tgcagcacct ggtcgaggag agccgtgcgg cgggcatcaa tggcgaactt 780
ccggcgtttg tgaccaaact ggccgatcgc gcagtggcag aaggtcgtgg tggtgactct 840
tacgctgcac tgattgaaca gtttcgcaag tcagcttaa                        879

 
<210> 44
<211> 292
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Imine reductase variant from Pseudonocardia spinosispora  
 
<400> 44
Met Ser Asp Ser Pro Val Pro Val Thr Val Leu Gly Leu Gly Leu Met 
1               5                   10                  15      
Gly Gln Ala Leu Ala Gly Ala Phe Leu Ala Ala Gly His Pro Thr Thr 
            20                  25                  30          
Val Trp Asn Arg Ser Ala Ala Lys Ala Asp Glu Leu Val Gly Arg Gly 
        35                  40                  45              
Ala Thr Leu Ala Ala Ser Pro Ala Ala Ala Val Ala Ala Gly Ser Leu 
    50                  55                  60                  
Ile Val Val Cys Val Thr Asp His Arg Ala Val Arg Glu Leu Leu Glu 
65                  70                  75                  80  
Pro Leu Gly Asp Ala Leu Pro Gly Arg Val Leu Val Asn Leu Thr Ser 
                85                  90                  95      
Gly Thr Ser Arg Glu Ala Arg Glu Asn Ala Glu His Thr Ala Ala Gln 
            100                 105                 110         
Gly Ala Thr Tyr Leu Asp Gly Val Ile Leu Thr Ile Pro Ser Gly Ile 
        115                 120                 125             
Gly Thr Pro Glu Ala Thr Ile Leu Tyr Ser Gly Pro Arg Ser Thr Phe 
    130                 135                 140                 
Asp Glu His Glu Ser Ala Leu Arg Ala Leu Gly Pro Gly Ala Thr Phe 
145                 150                 155                 160 
Val Gly Glu Asp His Gly Leu Ala Ala Leu Tyr Asp Ala Ala Met Leu 
                165                 170                 175     
Ser Val Met Trp Gly Leu Leu Asn Gly Phe Leu His Gly Thr Ala Leu 
            180                 185                 190         
Leu Gly Thr Ala Gly Val Ser Ala Thr Ser Val Val Pro Met Leu Gly 
        195                 200                 205             
Gln Gly Ile Glu Ala Val Thr Gly Ile Ile Ala Arg Tyr Ala Asp Gln 
    210                 215                 220                 
Ile Asp Ala Gly Val Tyr Pro Ala Asp Asp Ala Thr Ile Asp Thr Gln 
225                 230                 235                 240 
Arg Ala Ala Met Gln His Leu Val Glu Glu Ser Arg Ala Ala Gly Ile 
                245                 250                 255     
Asn Gly Glu Leu Pro Ala Phe Val Thr Lys Leu Ala Asp Arg Ala Val 
            260                 265                 270         
Ala Glu Gly Arg Gly Gly Asp Ser Tyr Ala Ala Leu Ile Glu Gln Phe 
        275                 280                 285             
Arg Lys Ser Ala                                                 
    290                                                         

 
<210> 45
<211> 879
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Imine reductase variant from Pseudonocardia spinosispora  
 
<400> 45
atgagtgatt cccccgtacc tgtgactgtt ctgggtcttg gtctgatggg tcaggcctta 60
gcgggagcgt ttctggcagc cggtcaccct acgacggttt ggaatcgcag cgcagctaaa 120
gctgatgaac tggttggtcg cggtgcaacc ctggccgcgt ctcccgcggc tgccgtcgca 180
gcgggtagtc tgatcgttgt ttgcgtcaca gatcatcgtg cggtgcgcga actgctggaa 240
ccacttggcg atgcactgcc aggtcgcgtt ctggtcaacc tgacgagcgg tacgtcgcgt 300
gaagcccgtg agaacgcaga acatactgcg gctcagggag ccacctacct ggatggtgtg 360
attctgacca ttccaagcgg cattggcacc ccagaagcta ccattctgta ttcgggaccg 420
cgcagtacct tcgatgaaca tgaaagcgca ctgcgcgcat taggtccggg ggcgaccttt 480
gtaggggaag atcacggact ggctagtctg tatgatgcag caatgctgag cgtcatgtgg 540
ggcctgctga atggtttcct gcatggaacc gcactgctgg gaaccgcggg cgtttcggca 600
acctctgtgg ttccgatgct gggtcaaggt atcgaagcgg tgaccggttg gattgcgcgc 660
tacgcagatc agattgatgc cggtgtctat ccagcagacg acgccacgat tgatacccag 720
cgtgctgcaa tgcagcacct ggtcgaggag agccgtgcgg cgggcatcaa tggcgaactt 780
ccggcgtttg tgaccaaact ggccgatcgc gcagtggcag aaggtcgtgg tggtgactct 840
tacgctgcac tgattgaaca gtttcgcaag tcagcttaa                        879

 
<210> 46
<211> 292
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Imine reductase variant from Pseudonocardia spinosispora  
 
<400> 46
Met Ser Asp Ser Pro Val Pro Val Thr Val Leu Gly Leu Gly Leu Met 
1               5                   10                  15      
Gly Gln Ala Leu Ala Gly Ala Phe Leu Ala Ala Gly His Pro Thr Thr 
            20                  25                  30          
Val Trp Asn Arg Ser Ala Ala Lys Ala Asp Glu Leu Val Gly Arg Gly 
        35                  40                  45              
Ala Thr Leu Ala Ala Ser Pro Ala Ala Ala Val Ala Ala Gly Ser Leu 
    50                  55                  60                  
Ile Val Val Cys Val Thr Asp His Arg Ala Val Arg Glu Leu Leu Glu 
65                  70                  75                  80  
Pro Leu Gly Asp Ala Leu Pro Gly Arg Val Leu Val Asn Leu Thr Ser 
                85                  90                  95      
Gly Thr Ser Arg Glu Ala Arg Glu Asn Ala Glu His Thr Ala Ala Gln 
            100                 105                 110         
Gly Ala Thr Tyr Leu Asp Gly Val Ile Leu Thr Ile Pro Ser Gly Ile 
        115                 120                 125             
Gly Thr Pro Glu Ala Thr Ile Leu Tyr Ser Gly Pro Arg Ser Thr Phe 
    130                 135                 140                 
Asp Glu His Glu Ser Ala Leu Arg Ala Leu Gly Pro Gly Ala Thr Phe 
145                 150                 155                 160 
Val Gly Glu Asp His Gly Leu Ala Ser Leu Tyr Asp Ala Ala Met Leu 
                165                 170                 175     
Ser Val Met Trp Gly Leu Leu Asn Gly Phe Leu His Gly Thr Ala Leu 
            180                 185                 190         
Leu Gly Thr Ala Gly Val Ser Ala Thr Ser Val Val Pro Met Leu Gly 
        195                 200                 205             
Gln Gly Ile Glu Ala Val Thr Gly Trp Ile Ala Arg Tyr Ala Asp Gln 
    210                 215                 220                 
Ile Asp Ala Gly Val Tyr Pro Ala Asp Asp Ala Thr Ile Asp Thr Gln 
225                 230                 235                 240 
Arg Ala Ala Met Gln His Leu Val Glu Glu Ser Arg Ala Ala Gly Ile 
                245                 250                 255     
Asn Gly Glu Leu Pro Ala Phe Val Thr Lys Leu Ala Asp Arg Ala Val 
            260                 265                 270         
Ala Glu Gly Arg Gly Gly Asp Ser Tyr Ala Ala Leu Ile Glu Gln Phe 
        275                 280                 285             
Arg Lys Ser Ala                                                 
    290                                                         

 
<210> 47
<211> 879
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Imine reductase variant from Pseudonocardia spinosispora  
 
<400> 47
atgagtgatt cccccgtacc tgtgactgtt ctgggtcttg gtctgatggg tcaggcctta 60
gcgggagcgt ttctggcagc cggtcaccct acgacggttt ggaatcgcag cgcagctaaa 120
gctgatgaac tggttggtcg cggtgcaacc ctggccgcgt ctcccgcggc tgccgtcgca 180
gcgggtagtc tgatcgttgt ttgcgtcaca gatcatcgtg cggtgcgcga actgctggaa 240
ccacttggcg atgcactgcc aggtcgcgtt ctggtcaacc tgacgagcgg tacgtcgcgt 300
gaagcccgtg agaacgcaga acatactgcg gctcagggag ccacctacct ggatggtgtg 360
attctgacca ttccaagcgg cattggcacc ccagaagcta ccattctgta ttcgggaccg 420
cgcagtacct tcgatgaaca tgaaagcgca ctgcgcgcat taggtccggg ggcgaccttt 480
gtaggggaag atcacggact ggctgcactg tatgatgcag cacatctgag cgtcatgtgg 540
ggcctgctga atggtttcct gcatggaacc gcactgctgg gaaccgcggg cgtttcggca 600
acctctgtgg ttccgatgct gggtcaaggt atcgaagcgg tgaccggttg gattgcgcgc 660
tacgcagatc agattgatgc cggtgtctat ccagcagacg acgccacgat tgatacccag 720
cgtgctgcaa tgcagcacct ggtcgaggag agccgtgcgg cgggcatcaa tggcgaactt 780
ccggcgtttg tgaccaaact ggccgatcgc gcagtggcag aaggtcgtgg tggtgactct 840
tacgctgcac tgattgaaca gtttcgcaag tcagcttaa                        879

 
<210> 48
<211> 292
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Imine reductase variant from Pseudonocardia spinosispora  
 
<400> 48
Met Ser Asp Ser Pro Val Pro Val Thr Val Leu Gly Leu Gly Leu Met 
1               5                   10                  15      
Gly Gln Ala Leu Ala Gly Ala Phe Leu Ala Ala Gly His Pro Thr Thr 
            20                  25                  30          
Val Trp Asn Arg Ser Ala Ala Lys Ala Asp Glu Leu Val Gly Arg Gly 
        35                  40                  45              
Ala Thr Leu Ala Ala Ser Pro Ala Ala Ala Val Ala Ala Gly Ser Leu 
    50                  55                  60                  
Ile Val Val Cys Val Thr Asp His Arg Ala Val Arg Glu Leu Leu Glu 
65                  70                  75                  80  
Pro Leu Gly Asp Ala Leu Pro Gly Arg Val Leu Val Asn Leu Thr Ser 
                85                  90                  95      
Gly Thr Ser Arg Glu Ala Arg Glu Asn Ala Glu His Thr Ala Ala Gln 
            100                 105                 110         
Gly Ala Thr Tyr Leu Asp Gly Val Ile Leu Thr Ile Pro Ser Gly Ile 
        115                 120                 125             
Gly Thr Pro Glu Ala Thr Ile Leu Tyr Ser Gly Pro Arg Ser Thr Phe 
    130                 135                 140                 
Asp Glu His Glu Ser Ala Leu Arg Ala Leu Gly Pro Gly Ala Thr Phe 
145                 150                 155                 160 
Val Gly Glu Asp His Gly Leu Ala Ala Leu Tyr Asp Ala Ala His Leu 
                165                 170                 175     
Ser Val Met Trp Gly Leu Leu Asn Gly Phe Leu His Gly Thr Ala Leu 
            180                 185                 190         
Leu Gly Thr Ala Gly Val Ser Ala Thr Ser Val Val Pro Met Leu Gly 
        195                 200                 205             
Gln Gly Ile Glu Ala Val Thr Gly Trp Ile Ala Arg Tyr Ala Asp Gln 
    210                 215                 220                 
Ile Asp Ala Gly Val Tyr Pro Ala Asp Asp Ala Thr Ile Asp Thr Gln 
225                 230                 235                 240 
Arg Ala Ala Met Gln His Leu Val Glu Glu Ser Arg Ala Ala Gly Ile 
                245                 250                 255     
Asn Gly Glu Leu Pro Ala Phe Val Thr Lys Leu Ala Asp Arg Ala Val 
            260                 265                 270         
Ala Glu Gly Arg Gly Gly Asp Ser Tyr Ala Ala Leu Ile Glu Gln Phe 
        275                 280                 285             
Arg Lys Ser Ala                                                 
    290                                                         

 
<210> 49
<211> 879
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Imine reductase variant from Pseudonocardia spinosispora  
 
<400> 49
atgagtgatt cccccgtacc tgtgactgtt ctgggtcttg gtctgatggg tcaggcctta 60
gcgggagcgt ttctggcagc cggtcaccct acgacggttt ggaatcgcag cgcagctaaa 120
gctgatgaac tggttggtcg cggtgcaacc ctggccgcgt ctcccgcggc tgccgtcgca 180
gcgggtagtc tgatcgttgt ttgcgtcaca gatcatcgtg cggtgcgcga actgctggaa 240
ccacttggcg atgcactgcc aggtcgcgtt ctggtcaacc tgacgagcgg tacgtcgcgt 300
gaagcccgtg agaacgcaga acatactgcg gctcagggag ccacctacct ggatggtgtg 360
attctgacca ttccaagcgg cattggcacc ccagaagcta ccattctgta ttcgggaccg 420
cgcagtacct tcgatgaaca tgaaagcgca ctgcgcgcat taggtccggg ggcgaccttt 480
gtaggggaag atcacggact ggctgcactg tatgatgcag caatgctgag cgtcatgtgg 540
ggcctgctga atggtttcct gcatggaacc gcactgctgg gaaccgcggg cgtttcggca 600
acctctgtgg ttccgatgct gggtcaaggt atcgaagcgg tgaccggttg gattgcgcgc 660
tacgcagatc agattgatgc cggtgtctat ccagcagacg acaaaacgat tgatacccag 720
cgtgctgcaa tgcagcacct ggtcgaggag agccgtgcgg cgggcatcaa tggcgaactt 780
ccggcgtttg tgaccaaact ggccgatcgc gcagtggcag aaggtcgtgg tggtgactct 840
tacgctgcac tgattgaaca gtttcgcaag tcagcttaa                        879

 
<210> 50
<211> 292
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Imine reductase variant from Pseudonocardia spinosispora  
 
<400> 50
Met Ser Asp Ser Pro Val Pro Val Thr Val Leu Gly Leu Gly Leu Met 
1               5                   10                  15      
Gly Gln Ala Leu Ala Gly Ala Phe Leu Ala Ala Gly His Pro Thr Thr 
            20                  25                  30          
Val Trp Asn Arg Ser Ala Ala Lys Ala Asp Glu Leu Val Gly Arg Gly 
        35                  40                  45              
Ala Thr Leu Ala Ala Ser Pro Ala Ala Ala Val Ala Ala Gly Ser Leu 
    50                  55                  60                  
Ile Val Val Cys Val Thr Asp His Arg Ala Val Arg Glu Leu Leu Glu 
65                  70                  75                  80  
Pro Leu Gly Asp Ala Leu Pro Gly Arg Val Leu Val Asn Leu Thr Ser 
                85                  90                  95      
Gly Thr Ser Arg Glu Ala Arg Glu Asn Ala Glu His Thr Ala Ala Gln 
            100                 105                 110         
Gly Ala Thr Tyr Leu Asp Gly Val Ile Leu Thr Ile Pro Ser Gly Ile 
        115                 120                 125             
Gly Thr Pro Glu Ala Thr Ile Leu Tyr Ser Gly Pro Arg Ser Thr Phe 
    130                 135                 140                 
Asp Glu His Glu Ser Ala Leu Arg Ala Leu Gly Pro Gly Ala Thr Phe 
145                 150                 155                 160 
Val Gly Glu Asp His Gly Leu Ala Ala Leu Tyr Asp Ala Ala Met Leu 
                165                 170                 175     
Ser Val Met Trp Gly Leu Leu Asn Gly Phe Leu His Gly Thr Ala Leu 
            180                 185                 190         
Leu Gly Thr Ala Gly Val Ser Ala Thr Ser Val Val Pro Met Leu Gly 
        195                 200                 205             
Gln Gly Ile Glu Ala Val Thr Gly Trp Ile Ala Arg Tyr Ala Asp Gln 
    210                 215                 220                 
Ile Asp Ala Gly Val Tyr Pro Ala Asp Asp Lys Thr Ile Asp Thr Gln 
225                 230                 235                 240 
Arg Ala Ala Met Gln His Leu Val Glu Glu Ser Arg Ala Ala Gly Ile 
                245                 250                 255     
Asn Gly Glu Leu Pro Ala Phe Val Thr Lys Leu Ala Asp Arg Ala Val 
            260                 265                 270         
Ala Glu Gly Arg Gly Gly Asp Ser Tyr Ala Ala Leu Ile Glu Gln Phe 
        275                 280                 285             
Arg Lys Ser Ala                                                 
    290                                                         

 
<210> 51
<211> 879
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Imine reductase variant from Pseudonocardia spinosispora  
 
<400> 51
atgagtgatt cccccgtacc tgtgactgtt ctgggtcttg gtctgatggg tcaggcctta 60
gcgggagcgt ttctggcagc cggtcaccct acgacggttt ggaatcgcag cgcagctaaa 120
gctgatgaac tggttggtcg cggtgcaacc ctggccgcgt ctcccgcggc tgccgtcgca 180
gcgggtagtc tgatcgttgt ttgcgtcaca gatcatcgtg cggtgcgcga actgctggaa 240
ccacttggcg atgcactgcc aggtcgcgtt ctggtcaacc tgacgagcgg tacgtcgcgt 300
gaagcccatg agaacgcaga acatactgcg gctcagggag ccacctacct ggatggtgtg 360
attctgacca ttccaagcgg cattggcacc ccagaagcta ccattctgta ttcgggaccg 420
cgcagtacct tcgatgaaca tgaaagcgca ctgcgcgcat taggtccggg ggcgaccttt 480
gtaggggaag atcacggact ggctgcactg tatgatgcag caatgctgag cgtcatgtgg 540
ggcctgctga atggtttcct gcatggaacc gcactgctgg gaaccgcggg cgtttcggca 600
acctctgtgg ttccgatgct gggtcaaggt atctgtgcgg tgaccggttg gattgcgcgc 660
tacgcagatc agattgatgc cggtgtctat ccagcagacg acgccacgat tgatacccag 720
cgtgctgcaa tgcagcacct ggtcgaggag agccgtgcgg cgggcatcaa tggcgaactt 780
ccggcgtttg tgaccaaact ggccgatcgc gcagtggcag aaggtcgtgg tggtgactct 840
tacgctgcac tgattgaaca gtttcgcaag tcagcttaa                        879

 
<210> 52
<211> 292
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Imine reductase variant from Pseudonocardia spinosispora  
 
<400> 52
Met Ser Asp Ser Pro Val Pro Val Thr Val Leu Gly Leu Gly Leu Met 
1               5                   10                  15      
Gly Gln Ala Leu Ala Gly Ala Phe Leu Ala Ala Gly His Pro Thr Thr 
            20                  25                  30          
Val Trp Asn Arg Ser Ala Ala Lys Ala Asp Glu Leu Val Gly Arg Gly 
        35                  40                  45              
Ala Thr Leu Ala Ala Ser Pro Ala Ala Ala Val Ala Ala Gly Ser Leu 
    50                  55                  60                  
Ile Val Val Cys Val Thr Asp His Arg Ala Val Arg Glu Leu Leu Glu 
65                  70                  75                  80  
Pro Leu Gly Asp Ala Leu Pro Gly Arg Val Leu Val Asn Leu Thr Ser 
                85                  90                  95      
Gly Thr Ser Arg Glu Ala His Glu Asn Ala Glu His Thr Ala Ala Gln 
            100                 105                 110         
Gly Ala Thr Tyr Leu Asp Gly Val Ile Leu Thr Ile Pro Ser Gly Ile 
        115                 120                 125             
Gly Thr Pro Glu Ala Thr Ile Leu Tyr Ser Gly Pro Arg Ser Thr Phe 
    130                 135                 140                 
Asp Glu His Glu Ser Ala Leu Arg Ala Leu Gly Pro Gly Ala Thr Phe 
145                 150                 155                 160 
Val Gly Glu Asp His Gly Leu Ala Ala Leu Tyr Asp Ala Ala Met Leu 
                165                 170                 175     
Ser Val Met Trp Gly Leu Leu Asn Gly Phe Leu His Gly Thr Ala Leu 
            180                 185                 190         
Leu Gly Thr Ala Gly Val Ser Ala Thr Ser Val Val Pro Met Leu Gly 
        195                 200                 205             
Gln Gly Ile Cys Ala Val Thr Gly Trp Ile Ala Arg Tyr Ala Asp Gln 
    210                 215                 220                 
Ile Asp Ala Gly Val Tyr Pro Ala Asp Asp Ala Thr Ile Asp Thr Gln 
225                 230                 235                 240 
Arg Ala Ala Met Gln His Leu Val Glu Glu Ser Arg Ala Ala Gly Ile 
                245                 250                 255     
Asn Gly Glu Leu Pro Ala Phe Val Thr Lys Leu Ala Asp Arg Ala Val 
            260                 265                 270         
Ala Glu Gly Arg Gly Gly Asp Ser Tyr Ala Ala Leu Ile Glu Gln Phe 
        275                 280                 285             
Arg Lys Ser Ala                                                 
    290                                                         

 
<210> 53
<211> 879
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Imine reductase variant from Pseudonocardia spinosispora  
 
<400> 53
atgagtgatt cccccgtacc tgtgactgtt ctgggtcttg gtctgatggg tcaggcctta 60
gcgggagcgt ttctggcagc cggtcaccct acgacggttt ggaatcgcag cgcagctaaa 120
gctgatgaac tggttggtcg cggtgcaacc ctggccgcgt ctcccgcggc tgccgtcgca 180
gcgggtagtc tgatcgttgt ttgcgtcaca gatcatcgtg cggtgcgcga actgctggaa 240
ccacttggcg atgcactgcc aggtcgcgtt ctggtcaacc tgacgagcgg tacgtcgcgt 300
gaagcccgtg agaacgcaga acatactgcg gctcagggag ccacctacct ggatggtgtg 360
attctgacca ttccaagcgg cattggcacc ccagaagcta ccattctgta ttcgggaccg 420
cgcagtacct tcgatgaaca tgaaagcgca ctgcgcgcat taggtccggg ggcgaccttt 480
gtaggggaag atcacggact ggctgcactg tatgatgcag caatgctgag cgtcatgtgg 540
ggcctgctga atggtttcct gcatggaacc gcactgctgg gaaccgcggg cgtttcggca 600
acctctgtgg ttccgatgct gggtcaaggt atcgaagcgg tgttgggttg gattgcgcgc 660
tacgcagatc agattgatgc cggtgtctat ccagcagacg acgccacgat tgatacccag 720
cgtgctgcaa tgcagcacct ggtcgaggag agccgtgcgg cgggcatcaa tggcgaactt 780
ccggcgtttg tgaccaaact ggccgatcgc gcagtggcag aaggtcgtgg tggtgactct 840
tacgctgcac tgattgaaca gtttcgcaag tcagcttaa                        879

 
<210> 54
<211> 292
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Imine reductase variant from Pseudonocardia spinosispora  
 
<400> 54
Met Ser Asp Ser Pro Val Pro Val Thr Val Leu Gly Leu Gly Leu Met 
1               5                   10                  15      
Gly Gln Ala Leu Ala Gly Ala Phe Leu Ala Ala Gly His Pro Thr Thr 
            20                  25                  30          
Val Trp Asn Arg Ser Ala Ala Lys Ala Asp Glu Leu Val Gly Arg Gly 
        35                  40                  45              
Ala Thr Leu Ala Ala Ser Pro Ala Ala Ala Val Ala Ala Gly Ser Leu 
    50                  55                  60                  
Ile Val Val Cys Val Thr Asp His Arg Ala Val Arg Glu Leu Leu Glu 
65                  70                  75                  80  
Pro Leu Gly Asp Ala Leu Pro Gly Arg Val Leu Val Asn Leu Thr Ser 
                85                  90                  95      
Gly Thr Ser Arg Glu Ala Arg Glu Asn Ala Glu His Thr Ala Ala Gln 
            100                 105                 110         
Gly Ala Thr Tyr Leu Asp Gly Val Ile Leu Thr Ile Pro Ser Gly Ile 
        115                 120                 125             
Gly Thr Pro Glu Ala Thr Ile Leu Tyr Ser Gly Pro Arg Ser Thr Phe 
    130                 135                 140                 
Asp Glu His Glu Ser Ala Leu Arg Ala Leu Gly Pro Gly Ala Thr Phe 
145                 150                 155                 160 
Val Gly Glu Asp His Gly Leu Ala Ala Leu Tyr Asp Ala Ala Met Leu 
                165                 170                 175     
Ser Val Met Trp Gly Leu Leu Asn Gly Phe Leu His Gly Thr Ala Leu 
            180                 185                 190         
Leu Gly Thr Ala Gly Val Ser Ala Thr Ser Val Val Pro Met Leu Gly 
        195                 200                 205             
Gln Gly Ile Glu Ala Val Leu Gly Trp Ile Ala Arg Tyr Ala Asp Gln 
    210                 215                 220                 
Ile Asp Ala Gly Val Tyr Pro Ala Asp Asp Ala Thr Ile Asp Thr Gln 
225                 230                 235                 240 
Arg Ala Ala Met Gln His Leu Val Glu Glu Ser Arg Ala Ala Gly Ile 
                245                 250                 255     
Asn Gly Glu Leu Pro Ala Phe Val Thr Lys Leu Ala Asp Arg Ala Val 
            260                 265                 270         
Ala Glu Gly Arg Gly Gly Asp Ser Tyr Ala Ala Leu Ile Glu Gln Phe 
        275                 280                 285             
Arg Lys Ser Ala                                                 
    290                                                         

 
<210> 55
<211> 879
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Imine reductase variant from Pseudonocardia spinosispora  
 
<400> 55
atgagtgatt cccccgtacc tgtgactgtt ctgggtcttg gtctgatggg tcaggcctta 60
gcgggagcgt ttctggcagc cggtcaccct acgacggttt ggaatcgcag cgcagctaaa 120
gctgatgaac tggttggtcg cggtgcaacc ctggccgcgt ctcccgcggc tgccgtcgca 180
gcgggtagtc tgatcgttgt ttgcgtcaca gatcatcgtg cggtgcgcga actgctggaa 240
ccacttggcg atgcactgcc aggtcgcgtt ctggtcaacc tgacgagcgg tacgtcgcgt 300
gaagcccgtg agaacgcaga acatactgcg gctcagggag ccacctacct ggatggtgtg 360
attctgacca ttccaagcgg cattggcacc ccagaagcta ccattctgta ttcgggaccg 420
cgcagtacct tcgatgaaca tgaaagcgca ctgcgcgcat taggtccggg ggcgaccttt 480
gtaggggaag atcacggact ggctgcactg tatgatgcag caatgctgag catgatgtgg 540
ggcctgctga atggtttcct gcatggaacc gcactgctgg gaaccgcggg cgtttcggca 600
acctctgtgg ttccgatgct gggtcaaggt atcgaagcgg tgaccggttg gattgcgcgc 660
tacgcagatc agattgatgc cggtgtctat ccagcagacg acgccacgat tgatacccag 720
cgtgctgcaa tgcagcacct ggtcgaggag agccgtgcgg cgggcatcaa tggcgaactt 780
ccggcgtttg tgaccaaact ggccgatcgc gcagtggcag aaggtcgtgg tggtgactct 840
tacgctgcac tgattgaaca gtttcgcaag tcagcttaa                        879

 
<210> 56
<211> 292
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Imine reductase variant from Pseudonocardia spinosispora  
 
<400> 56
Met Ser Asp Ser Pro Val Pro Val Thr Val Leu Gly Leu Gly Leu Met 
1               5                   10                  15      
Gly Gln Ala Leu Ala Gly Ala Phe Leu Ala Ala Gly His Pro Thr Thr 
            20                  25                  30          
Val Trp Asn Arg Ser Ala Ala Lys Ala Asp Glu Leu Val Gly Arg Gly 
        35                  40                  45              
Ala Thr Leu Ala Ala Ser Pro Ala Ala Ala Val Ala Ala Gly Ser Leu 
    50                  55                  60                  
Ile Val Val Cys Val Thr Asp His Arg Ala Val Arg Glu Leu Leu Glu 
65                  70                  75                  80  
Pro Leu Gly Asp Ala Leu Pro Gly Arg Val Leu Val Asn Leu Thr Ser 
                85                  90                  95      
Gly Thr Ser Arg Glu Ala Arg Glu Asn Ala Glu His Thr Ala Ala Gln 
            100                 105                 110         
Gly Ala Thr Tyr Leu Asp Gly Val Ile Leu Thr Ile Pro Ser Gly Ile 
        115                 120                 125             
Gly Thr Pro Glu Ala Thr Ile Leu Tyr Ser Gly Pro Arg Ser Thr Phe 
    130                 135                 140                 
Asp Glu His Glu Ser Ala Leu Arg Ala Leu Gly Pro Gly Ala Thr Phe 
145                 150                 155                 160 
Val Gly Glu Asp His Gly Leu Ala Ala Leu Tyr Asp Ala Ala Met Leu 
                165                 170                 175     
Ser Met Met Trp Gly Leu Leu Asn Gly Phe Leu His Gly Thr Ala Leu 
            180                 185                 190         
Leu Gly Thr Ala Gly Val Ser Ala Thr Ser Val Val Pro Met Leu Gly 
        195                 200                 205             
Gln Gly Ile Glu Ala Val Thr Gly Trp Ile Ala Arg Tyr Ala Asp Gln 
    210                 215                 220                 
Ile Asp Ala Gly Val Tyr Pro Ala Asp Asp Ala Thr Ile Asp Thr Gln 
225                 230                 235                 240 
Arg Ala Ala Met Gln His Leu Val Glu Glu Ser Arg Ala Ala Gly Ile 
                245                 250                 255     
Asn Gly Glu Leu Pro Ala Phe Val Thr Lys Leu Ala Asp Arg Ala Val 
            260                 265                 270         
Ala Glu Gly Arg Gly Gly Asp Ser Tyr Ala Ala Leu Ile Glu Gln Phe 
        275                 280                 285             
Arg Lys Ser Ala                                                 
    290                                                         

 
<210> 57
<211> 879
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Imine reductase variant from Pseudonocardia spinosispora  
 
<400> 57
atgagtgatt cccccgtacc tgtgactgtt ctgggtcttg gtctgatggg tcaggcctta 60
gcgggagcgt ttctggcagc cggtcaccct acgacggttt ggaatcgcag cgcagctaaa 120
gctgatgaac tggttggtcg cggtgcaacc ctggccgcgt ctcccgcggc tgccgtcgca 180
gcgggtagtc tgatcgttgt ttgcgtcaca gatcatcgtg cggtgcgcga actgctggaa 240
ccacttggcg atgcactgcc aggtcgcgtt ctggtcaacc tgacgagcgg tacgtcgcgt 300
gaagcccgtg agaacgcaga acatactgcg gctcagggag ccacctacct ggatggtgtg 360
attctgacca ttccaagcgg cattggcacc ccagaagcta ccattctgta ttcgggaccg 420
cgcagtacct tcgatgaaca tgaaagcgca ctgcgcgcat taggtccggg ggcgaccttt 480
gtaggggaag atcacggact ggctgcactg tatgatgcag caatgctgag cgtcatgtgg 540
ggcctgctga atggtttcct gcatggaacc gcactgctgg gaaccgcggg cgtttcggca 600
acctctgtgg ttccgatgct gggtcaaggt atctgtgcgg tgaccggttg gattgcgcgc 660
tacgcagatc agattgatgc cggtgtctat ccagcagacg acgccacgat tgatacccag 720
cgtgctgcaa tgcagcacct ggtcgaggag agccgtgcgg cgggcatcaa tggcgaactt 780
ccggcgtttg tgaccaaact ggccgatcgc gcagtggcag aaggtcgtgg tggtgactct 840
tacgctgcac tgattgaaca gtttcgcaag tcagcttaa                        879

 
<210> 58
<211> 292
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Imine reductase variant from Pseudonocardia spinosispora  
 
<400> 58
Met Ser Asp Ser Pro Val Pro Val Thr Val Leu Gly Leu Gly Leu Met 
1               5                   10                  15      
Gly Gln Ala Leu Ala Gly Ala Phe Leu Ala Ala Gly His Pro Thr Thr 
            20                  25                  30          
Val Trp Asn Arg Ser Ala Ala Lys Ala Asp Glu Leu Val Gly Arg Gly 
        35                  40                  45              
Ala Thr Leu Ala Ala Ser Pro Ala Ala Ala Val Ala Ala Gly Ser Leu 
    50                  55                  60                  
Ile Val Val Cys Val Thr Asp His Arg Ala Val Arg Glu Leu Leu Glu 
65                  70                  75                  80  
Pro Leu Gly Asp Ala Leu Pro Gly Arg Val Leu Val Asn Leu Thr Ser 
                85                  90                  95      
Gly Thr Ser Arg Glu Ala Arg Glu Asn Ala Glu His Thr Ala Ala Gln 
            100                 105                 110         
Gly Ala Thr Tyr Leu Asp Gly Val Ile Leu Thr Ile Pro Ser Gly Ile 
        115                 120                 125             
Gly Thr Pro Glu Ala Thr Ile Leu Tyr Ser Gly Pro Arg Ser Thr Phe 
    130                 135                 140                 
Asp Glu His Glu Ser Ala Leu Arg Ala Leu Gly Pro Gly Ala Thr Phe 
145                 150                 155                 160 
Val Gly Glu Asp His Gly Leu Ala Ala Leu Tyr Asp Ala Ala Met Leu 
                165                 170                 175     
Ser Val Met Trp Gly Leu Leu Asn Gly Phe Leu His Gly Thr Ala Leu 
            180                 185                 190         
Leu Gly Thr Ala Gly Val Ser Ala Thr Ser Val Val Pro Met Leu Gly 
        195                 200                 205             
Gln Gly Ile Cys Ala Val Thr Gly Trp Ile Ala Arg Tyr Ala Asp Gln 
    210                 215                 220                 
Ile Asp Ala Gly Val Tyr Pro Ala Asp Asp Ala Thr Ile Asp Thr Gln 
225                 230                 235                 240 
Arg Ala Ala Met Gln His Leu Val Glu Glu Ser Arg Ala Ala Gly Ile 
                245                 250                 255     
Asn Gly Glu Leu Pro Ala Phe Val Thr Lys Leu Ala Asp Arg Ala Val 
            260                 265                 270         
Ala Glu Gly Arg Gly Gly Asp Ser Tyr Ala Ala Leu Ile Glu Gln Phe 
        275                 280                 285             
Arg Lys Ser Ala                                                 
    290                                                         

 
<210> 59
<211> 879
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Imine reductase variant from Pseudonocardia spinosispora  
 
<400> 59
atgagtgatt cccccgtacc tgtgactgtt ctgggtcttg gtctgatggg tcaggcctta 60
gcgggagcgt ttctggcagc cggtcaccct acgacggttt ggaatcgcag cgcagctaaa 120
gctgatgaac tggttggtcg cggtgcaacc ctggccgcgt ctcccgcggc tgccgtcgca 180
gcgggtagtc tgatcgttgt ttgcgtcaca gatcatcgtg cggtgcgcga actgctggaa 240
ccacttggcg atgcactgcc aggtcgcgtt ctggtcaacc tgacgagcgg tacgtcgcgt 300
gaagcccgtg agaacgcaga acatactgcg gctcagggag ccacctacct ggatggtgtg 360
attctgacca ttccaagcgg cattggcacc ccagaagcta ggattctgta ttcgggaccg 420
cgcagtacct tcgatgaaca tgaaagcgca ctgcgcgcat taggtccggg ggcgaccttt 480
gtaggggaag atcacggact ggctgcactg tatgatgcag caatgctgag cgtcatgtgg 540
ggcctgctga atggtttcct gcatggaacc gcactgctgg gaaccgcggg cgtttcggca 600
acctctgtgg ttccgatgct gggtcaaggt atcgaagcgg tgaccggttg gattgcgcgc 660
tacgcagatc agattgatgc cggtgtctat ccagcagacg acgccacgat tgatacccag 720
cgtgctgcaa tgcagcacct ggtcgaggag agccgtgcgg cgggcatcaa tggcgaactt 780
ccggcgtttg tgaccaaact ggccgatcgc gcagtggcag aaggtcgtgg tggtgactct 840
tacgctgcac tgattgaaca gtttcgcaag tcagcttaa                        879

 
<210> 60
<211> 292
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Imine reductase variant from Pseudonocardia spinosispora  
 
<400> 60
Met Ser Asp Ser Pro Val Pro Val Thr Val Leu Gly Leu Gly Leu Met 
1               5                   10                  15      
Gly Gln Ala Leu Ala Gly Ala Phe Leu Ala Ala Gly His Pro Thr Thr 
            20                  25                  30          
Val Trp Asn Arg Ser Ala Ala Lys Ala Asp Glu Leu Val Gly Arg Gly 
        35                  40                  45              
Ala Thr Leu Ala Ala Ser Pro Ala Ala Ala Val Ala Ala Gly Ser Leu 
    50                  55                  60                  
Ile Val Val Cys Val Thr Asp His Arg Ala Val Arg Glu Leu Leu Glu 
65                  70                  75                  80  
Pro Leu Gly Asp Ala Leu Pro Gly Arg Val Leu Val Asn Leu Thr Ser 
                85                  90                  95      
Gly Thr Ser Arg Glu Ala Arg Glu Asn Ala Glu His Thr Ala Ala Gln 
            100                 105                 110         
Gly Ala Thr Tyr Leu Asp Gly Val Ile Leu Thr Ile Pro Ser Gly Ile 
        115                 120                 125             
Gly Thr Pro Glu Ala Arg Ile Leu Tyr Ser Gly Pro Arg Ser Thr Phe 
    130                 135                 140                 
Asp Glu His Glu Ser Ala Leu Arg Ala Leu Gly Pro Gly Ala Thr Phe 
145                 150                 155                 160 
Val Gly Glu Asp His Gly Leu Ala Ala Leu Tyr Asp Ala Ala Met Leu 
                165                 170                 175     
Ser Val Met Trp Gly Leu Leu Asn Gly Phe Leu His Gly Thr Ala Leu 
            180                 185                 190         
Leu Gly Thr Ala Gly Val Ser Ala Thr Ser Val Val Pro Met Leu Gly 
        195                 200                 205             
Gln Gly Ile Glu Ala Val Thr Gly Trp Ile Ala Arg Tyr Ala Asp Gln 
    210                 215                 220                 
Ile Asp Ala Gly Val Tyr Pro Ala Asp Asp Ala Thr Ile Asp Thr Gln 
225                 230                 235                 240 
Arg Ala Ala Met Gln His Leu Val Glu Glu Ser Arg Ala Ala Gly Ile 
                245                 250                 255     
Asn Gly Glu Leu Pro Ala Phe Val Thr Lys Leu Ala Asp Arg Ala Val 
            260                 265                 270         
Ala Glu Gly Arg Gly Gly Asp Ser Tyr Ala Ala Leu Ile Glu Gln Phe 
        275                 280                 285             
Arg Lys Ser Ala                                                 
    290                                                         

 
<210> 61
<211> 879
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Imine reductase variant from Pseudonocardia spinosispora  
 
<400> 61
atgagtgatt cccccgtacc tgtgactgtt ctgggtcttg gtctgatggg tcaggcctta 60
gcgggagcgt ttctggcagc cggtcaccct acgacggttt ggaatcgcag cgcagctaaa 120
gctgatgaac tggttggtcg cggtgcaacc ctggccgcgt ctcccgcggc tgccgtcgca 180
gcgggtagtc tgatcgttgt ttgcgtcaca gatcatcgtg cggtgcgcga actgctggaa 240
ccacttggcg atgcactgcc aggtcgcgtt ctggtcaacc tgacgagcgg tacgtcgcgt 300
gaagcccgtg agaacgcaga acatactgcg gctcagggag ccacctacct ggatggtgtg 360
attctgacca ttccaagcgg cattggcacc ccagaagcta ccattctgta ttcgggaccg 420
cgcagtacct tcgatgaaca tgaaagcgca ctgcgcgcat taggtccggg ggcgaccttt 480
gtaggggaag atcacggact ggctgcactg tatgatgcag caatgctgag cgtcatgtgg 540
ggcctgctga atggtttcct gcatggaacc gcactgctgg gaaccgcggg cgtttcggca 600
acctctgtgg ttccgatgct gggtcaaggt atcgttgcgg tgaccggttg gattgcgcgc 660
tacgcagatc agattgatgc cggtgtctat ccagcagacg acgccacgat tgatacccag 720
cgtgctgcaa tgcagcacct ggtcgaggag agccgtgcgg cgggcatcaa tggcgaactt 780
ccggcgtttg tgaccaaact ggccgatcgc gcagtggcag aaggtcgtgg tggtgactct 840
tacgctgcac tgattgaaca gtttcgcaag tcagcttaa                        879

 
<210> 62
<211> 292
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Imine reductase variant from Pseudonocardia spinosispora  
 
<400> 62
Met Ser Asp Ser Pro Val Pro Val Thr Val Leu Gly Leu Gly Leu Met 
1               5                   10                  15      
Gly Gln Ala Leu Ala Gly Ala Phe Leu Ala Ala Gly His Pro Thr Thr 
            20                  25                  30          
Val Trp Asn Arg Ser Ala Ala Lys Ala Asp Glu Leu Val Gly Arg Gly 
        35                  40                  45              
Ala Thr Leu Ala Ala Ser Pro Ala Ala Ala Val Ala Ala Gly Ser Leu 
    50                  55                  60                  
Ile Val Val Cys Val Thr Asp His Arg Ala Val Arg Glu Leu Leu Glu 
65                  70                  75                  80  
Pro Leu Gly Asp Ala Leu Pro Gly Arg Val Leu Val Asn Leu Thr Ser 
                85                  90                  95      
Gly Thr Ser Arg Glu Ala Arg Glu Asn Ala Glu His Thr Ala Ala Gln 
            100                 105                 110         
Gly Ala Thr Tyr Leu Asp Gly Val Ile Leu Thr Ile Pro Ser Gly Ile 
        115                 120                 125             
Gly Thr Pro Glu Ala Thr Ile Leu Tyr Ser Gly Pro Arg Ser Thr Phe 
    130                 135                 140                 
Asp Glu His Glu Ser Ala Leu Arg Ala Leu Gly Pro Gly Ala Thr Phe 
145                 150                 155                 160 
Val Gly Glu Asp His Gly Leu Ala Ala Leu Tyr Asp Ala Ala Met Leu 
                165                 170                 175     
Ser Val Met Trp Gly Leu Leu Asn Gly Phe Leu His Gly Thr Ala Leu 
            180                 185                 190         
Leu Gly Thr Ala Gly Val Ser Ala Thr Ser Val Val Pro Met Leu Gly 
        195                 200                 205             
Gln Gly Ile Val Ala Val Thr Gly Trp Ile Ala Arg Tyr Ala Asp Gln 
    210                 215                 220                 
Ile Asp Ala Gly Val Tyr Pro Ala Asp Asp Ala Thr Ile Asp Thr Gln 
225                 230                 235                 240 
Arg Ala Ala Met Gln His Leu Val Glu Glu Ser Arg Ala Ala Gly Ile 
                245                 250                 255     
Asn Gly Glu Leu Pro Ala Phe Val Thr Lys Leu Ala Asp Arg Ala Val 
            260                 265                 270         
Ala Glu Gly Arg Gly Gly Asp Ser Tyr Ala Ala Leu Ile Glu Gln Phe 
        275                 280                 285             
Arg Lys Ser Ala                                                 
    290                                                         

 
<210> 63
<211> 879
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Imine reductase variant from Pseudonocardia spinosispora  
 
<400> 63
atgagtgatt cccccgtacc tgtgactgtt ctgggtcttg gtctgatggg tcaggcctta 60
gcgggagcgt ttctggcagc cggtcaccct acgacggttt ggaatcgcag cgcagctaaa 120
gctgatgaac tggttggtcg cggtgcaacc ctggccgcgt ctcccgcggc tgccgtcgca 180
gcgggtagtc tgatcgttgt ttgcgtcaca gatcatcgtg cggtgcgcga actgctggaa 240
ccacttggcg atgcactgcc aggtcgcgtt ctggtcaacc tgacgagcgg tacgtcgcgt 300
gaagcccgtg agaacgcaga acatactgcg gctcagggag ccacctacct ggatggtgtg 360
attctgacca ttccaagcgg cattggcacc ccagaagcta ccattctgta ttcgggaccg 420
cgcagtacct tcgatgaaca tgaaagcgca ctgcgcgcat taggtccggg ggcgaccttt 480
gtaggggaag atcacggact ggctgcactg tatgatgcag caatgctgag cgtcatgtgg 540
ggcctgctga atggtttcct gcatggaacc gcactgctgg gaaccgcggg cgtttcggca 600
acctctgtgg ttccgatgct gggtcaaggt atcgaagcgg tgatgggttg gattgcgcgc 660
tacgcagatc agattgatgc cggtgtctat ccagcagacg acgccacgat tgatacccag 720
cgtgctgcaa tgcagcacct ggtcgaggag agccgtgcgg cgggcatcaa tggcgaactt 780
ccggcgtttg tgaccaaact ggccgatcgc gcagtggcag aaggtcgtgg tggtgactct 840
tacgctgcac tgattgaaca gtttcgcaag tcagcttaa                        879

 
<210> 64
<211> 292
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Imine reductase variant from Pseudonocardia spinosispora  
 
<400> 64
Met Ser Asp Ser Pro Val Pro Val Thr Val Leu Gly Leu Gly Leu Met 
1               5                   10                  15      
Gly Gln Ala Leu Ala Gly Ala Phe Leu Ala Ala Gly His Pro Thr Thr 
            20                  25                  30          
Val Trp Asn Arg Ser Ala Ala Lys Ala Asp Glu Leu Val Gly Arg Gly 
        35                  40                  45              
Ala Thr Leu Ala Ala Ser Pro Ala Ala Ala Val Ala Ala Gly Ser Leu 
    50                  55                  60                  
Ile Val Val Cys Val Thr Asp His Arg Ala Val Arg Glu Leu Leu Glu 
65                  70                  75                  80  
Pro Leu Gly Asp Ala Leu Pro Gly Arg Val Leu Val Asn Leu Thr Ser 
                85                  90                  95      
Gly Thr Ser Arg Glu Ala Arg Glu Asn Ala Glu His Thr Ala Ala Gln 
            100                 105                 110         
Gly Ala Thr Tyr Leu Asp Gly Val Ile Leu Thr Ile Pro Ser Gly Ile 
        115                 120                 125             
Gly Thr Pro Glu Ala Thr Ile Leu Tyr Ser Gly Pro Arg Ser Thr Phe 
    130                 135                 140                 
Asp Glu His Glu Ser Ala Leu Arg Ala Leu Gly Pro Gly Ala Thr Phe 
145                 150                 155                 160 
Val Gly Glu Asp His Gly Leu Ala Ala Leu Tyr Asp Ala Ala Met Leu 
                165                 170                 175     
Ser Val Met Trp Gly Leu Leu Asn Gly Phe Leu His Gly Thr Ala Leu 
            180                 185                 190         
Leu Gly Thr Ala Gly Val Ser Ala Thr Ser Val Val Pro Met Leu Gly 
        195                 200                 205             
Gln Gly Ile Glu Ala Val Met Gly Trp Ile Ala Arg Tyr Ala Asp Gln 
    210                 215                 220                 
Ile Asp Ala Gly Val Tyr Pro Ala Asp Asp Ala Thr Ile Asp Thr Gln 
225                 230                 235                 240 
Arg Ala Ala Met Gln His Leu Val Glu Glu Ser Arg Ala Ala Gly Ile 
                245                 250                 255     
Asn Gly Glu Leu Pro Ala Phe Val Thr Lys Leu Ala Asp Arg Ala Val 
            260                 265                 270         
Ala Glu Gly Arg Gly Gly Asp Ser Tyr Ala Ala Leu Ile Glu Gln Phe 
        275                 280                 285             
Arg Lys Ser Ala                                                 
    290                                                         

 
<210> 65
<211> 879
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Imine reductase variant from Pseudonocardia spinosispora  
 
<400> 65
atgagtgatt cccccgtacc tgtgactgtt ctgggtcttg gtctgatggg tcaggcctta 60
gcgggagcgt ttctggcagc cggtcaccct acgacggttt ggaatcgcag cgcagctaaa 120
gctgatgaac tggttggtcg cggtgcaacc ctggccgcgt ctcccgcggc tgccgtcgca 180
gcgggtagtc tgatcgttgt ttgcgtcaca gatcatcgtg cggtgcgcga actgctggaa 240
ccacttggcg atgcactgcc aggtcgcgtt ctggtcaacc tgacgagcgg tacgtcgcgt 300
gaagcccgtg agaacgcaga acatactgcg gctcagggag ccacctacct ggatggtgtg 360
attctgacca ttccaagcgg cattggcacc ccagaagcta ccattctgta ttcgggaccg 420
cgcagtacct tcgatgaaca tgaaagcgca ctgcgcgcat taggtccggg ggcgaccttt 480
gtaggggaag atcacggact ggctgcactg tatgatgcag caatgctgag cgtcatgtgg 540
ggcctgctga atggtttcct gcatggaacc gcactgctgg gaaccgcggg cgtttcggca 600
acctctgtgg ttccgatgct gggtcaaggt tgggaagcgg tgaccggttg gattgcgcgc 660
tacgcagatc agattgatgc cggtgtctat ccagcagacg acgccacgat tgatacccag 720
cgtgctgcaa tgcagcacct ggtcgaggag agccgtgcgg cgggcatcaa tggcgaactt 780
ccggcgtttg tgaccaaact ggccgatcgc gcagtggcag aaggtcgtgg tggtgactct 840
tacgctgcac tgattgaaca gtttcgcaag tcagcttaa                        879

 
<210> 66
<211> 292
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Imine reductase variant from Pseudonocardia spinosispora  
 
<400> 66
Met Ser Asp Ser Pro Val Pro Val Thr Val Leu Gly Leu Gly Leu Met 
1               5                   10                  15      
Gly Gln Ala Leu Ala Gly Ala Phe Leu Ala Ala Gly His Pro Thr Thr 
            20                  25                  30          
Val Trp Asn Arg Ser Ala Ala Lys Ala Asp Glu Leu Val Gly Arg Gly 
        35                  40                  45              
Ala Thr Leu Ala Ala Ser Pro Ala Ala Ala Val Ala Ala Gly Ser Leu 
    50                  55                  60                  
Ile Val Val Cys Val Thr Asp His Arg Ala Val Arg Glu Leu Leu Glu 
65                  70                  75                  80  
Pro Leu Gly Asp Ala Leu Pro Gly Arg Val Leu Val Asn Leu Thr Ser 
                85                  90                  95      
Gly Thr Ser Arg Glu Ala Arg Glu Asn Ala Glu His Thr Ala Ala Gln 
            100                 105                 110         
Gly Ala Thr Tyr Leu Asp Gly Val Ile Leu Thr Ile Pro Ser Gly Ile 
        115                 120                 125             
Gly Thr Pro Glu Ala Thr Ile Leu Tyr Ser Gly Pro Arg Ser Thr Phe 
    130                 135                 140                 
Asp Glu His Glu Ser Ala Leu Arg Ala Leu Gly Pro Gly Ala Thr Phe 
145                 150                 155                 160 
Val Gly Glu Asp His Gly Leu Ala Ala Leu Tyr Asp Ala Ala Met Leu 
                165                 170                 175     
Ser Val Met Trp Gly Leu Leu Asn Gly Phe Leu His Gly Thr Ala Leu 
            180                 185                 190         
Leu Gly Thr Ala Gly Val Ser Ala Thr Ser Val Val Pro Met Leu Gly 
        195                 200                 205             
Gln Gly Trp Glu Ala Val Thr Gly Trp Ile Ala Arg Tyr Ala Asp Gln 
    210                 215                 220                 
Ile Asp Ala Gly Val Tyr Pro Ala Asp Asp Ala Thr Ile Asp Thr Gln 
225                 230                 235                 240 
Arg Ala Ala Met Gln His Leu Val Glu Glu Ser Arg Ala Ala Gly Ile 
                245                 250                 255     
Asn Gly Glu Leu Pro Ala Phe Val Thr Lys Leu Ala Asp Arg Ala Val 
            260                 265                 270         
Ala Glu Gly Arg Gly Gly Asp Ser Tyr Ala Ala Leu Ile Glu Gln Phe 
        275                 280                 285             
Arg Lys Ser Ala                                                 
    290                                                         

 
<210> 67
<211> 879
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Imine reductase variant from Pseudonocardia spinosispora  
 
<400> 67
atgagtgatt cccccgtacc tgtgactgtt ctgggtcttg gtctgatggg tcaggcctta 60
gcgggagcgt ttctggcagc cggtcaccct acgacggttt ggaatcgcag cgcagctaaa 120
gctgatgaac tggttggtcg cggtgcaacc ctggccgcgt ctcccgcgac tgccgtcgca 180
gcgggtagtc tgatcgttgt ttgcgtcaca gatcatcgtg cggtgcgcga actgctggaa 240
ccacttggcg atgcactgcc aggtcgcgtt ctggtcaacc tgacgagcgg tacgtcgcgt 300
gaagcccgtg agaacgcaga acatactgcg gctcagggag ccacctacct ggatggtgtg 360
attctgacca ttccaagcgg cattggcacc ccagaagcta ccattctgta ttcgggaccg 420
cgcagtacct tcgatgaaca tgaaagcgca ctgcgcgcat taggtccggg ggcgaccttt 480
gtaggggaag atcacggact ggctgcactg tatgatgcag caatgctgag cgtcatgtgg 540
ggcctgctga atggtttcct gcatggaacc gcactgctgg gaaccgcggg cgtttcggca 600
acctctgtgg ttccgatgct gggtcaaggt cttgaagcgg tgaccggttg gattgcgcgc 660
tacgcagatc agattgatgc cggtgtctat ccagcagacg acgccacgat tgatacccag 720
cgtgctgcaa tgcagcacct ggtcgaggag agccgtgcgg cgggcatcaa tggcgaactt 780
ccggcgtttg tgaccaaact ggccgatcgc gcagtggcag aaggtcgtgg tggtgactct 840
tacgctgcac tgattgaaca gtttcgcaag tcagcttaa                        879

 
<210> 68
<211> 292
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Imine reductase variant from Pseudonocardia spinosispora  
 
<400> 68
Met Ser Asp Ser Pro Val Pro Val Thr Val Leu Gly Leu Gly Leu Met 
1               5                   10                  15      
Gly Gln Ala Leu Ala Gly Ala Phe Leu Ala Ala Gly His Pro Thr Thr 
            20                  25                  30          
Val Trp Asn Arg Ser Ala Ala Lys Ala Asp Glu Leu Val Gly Arg Gly 
        35                  40                  45              
Ala Thr Leu Ala Ala Ser Pro Ala Thr Ala Val Ala Ala Gly Ser Leu 
    50                  55                  60                  
Ile Val Val Cys Val Thr Asp His Arg Ala Val Arg Glu Leu Leu Glu 
65                  70                  75                  80  
Pro Leu Gly Asp Ala Leu Pro Gly Arg Val Leu Val Asn Leu Thr Ser 
                85                  90                  95      
Gly Thr Ser Arg Glu Ala Arg Glu Asn Ala Glu His Thr Ala Ala Gln 
            100                 105                 110         
Gly Ala Thr Tyr Leu Asp Gly Val Ile Leu Thr Ile Pro Ser Gly Ile 
        115                 120                 125             
Gly Thr Pro Glu Ala Thr Ile Leu Tyr Ser Gly Pro Arg Ser Thr Phe 
    130                 135                 140                 
Asp Glu His Glu Ser Ala Leu Arg Ala Leu Gly Pro Gly Ala Thr Phe 
145                 150                 155                 160 
Val Gly Glu Asp His Gly Leu Ala Ala Leu Tyr Asp Ala Ala Met Leu 
                165                 170                 175     
Ser Val Met Trp Gly Leu Leu Asn Gly Phe Leu His Gly Thr Ala Leu 
            180                 185                 190         
Leu Gly Thr Ala Gly Val Ser Ala Thr Ser Val Val Pro Met Leu Gly 
        195                 200                 205             
Gln Gly Leu Glu Ala Val Thr Gly Trp Ile Ala Arg Tyr Ala Asp Gln 
    210                 215                 220                 
Ile Asp Ala Gly Val Tyr Pro Ala Asp Asp Ala Thr Ile Asp Thr Gln 
225                 230                 235                 240 
Arg Ala Ala Met Gln His Leu Val Glu Glu Ser Arg Ala Ala Gly Ile 
                245                 250                 255     
Asn Gly Glu Leu Pro Ala Phe Val Thr Lys Leu Ala Asp Arg Ala Val 
            260                 265                 270         
Ala Glu Gly Arg Gly Gly Asp Ser Tyr Ala Ala Leu Ile Glu Gln Phe 
        275                 280                 285             
Arg Lys Ser Ala                                                 
    290                                                         

 
<210> 69
<211> 879
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Imine reductase variant from Pseudonocardia spinosispora  
 
<400> 69
atgagtgatt cccccgtacc tgtgactgtt ctgggtcttg gtctgatggg tcaggcctta 60
gcgggagcgt ttctggcagc cggtcaccct acgacggttt ggaatcgcag cgcagctaaa 120
gctgatgaac tggttggtcg cggtgcaacc ctggccgcgt ctcccgcggc tgccgtcgca 180
gcgggtagtc tgatcgttgt ttgcgtcaca gatcatcgtg cggtgcgcga actgctggaa 240
ccacttggcg atgcactgcc aggtcgcgtt ctggtcaacc tgacgagcgg tacgtcgcgt 300
gaagcccgtg agaacgcaga acatactgcg gctcagggag ccacctacct ggatggtgtg 360
attctgacca ttccaagcgg cattggcacc ccagaagcta ccattctgta ttcgggaccg 420
cgcagtacct tcgatgaaca tgaaagcgca ctgcgcgcat taggtccggg ggcgaccttt 480
gtaggggaag atcacggact ggctgcactg tatgatgcag caatgctgag cgtcatgtgg 540
ggcctgctga atggtttcct gcatggaacc gcactgctgg gaaccgcggg cgtttcggca 600
acctctgtgg ttccgatgct gggtcaaggt cttgaagcgg tgaccggttg gattgcgcgc 660
tacgcagatc agattgatgc cggtgtctat ccagcagacg acgccacgat tgatacccag 720
cgtgctgcaa tgcagcacct ggtcgaggag agccgtgcgg cgggcatcaa tggcgaactt 780
ccggcgtttg tgaccaaact ggccgatcgc gcagtggcag aaggtcgtgg tggtgactct 840
tacgctgcac tgattgaaca gtttcgcaag tcagcttaa                        879

 
<210> 70
<211> 292
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Imine reductase variant from Pseudonocardia spinosispora  
 
<400> 70
Met Ser Asp Ser Pro Val Pro Val Thr Val Leu Gly Leu Gly Leu Met 
1               5                   10                  15      
Gly Gln Ala Leu Ala Gly Ala Phe Leu Ala Ala Gly His Pro Thr Thr 
            20                  25                  30          
Val Trp Asn Arg Ser Ala Ala Lys Ala Asp Glu Leu Val Gly Arg Gly 
        35                  40                  45              
Ala Thr Leu Ala Ala Ser Pro Ala Ala Ala Val Ala Ala Gly Ser Leu 
    50                  55                  60                  
Ile Val Val Cys Val Thr Asp His Arg Ala Val Arg Glu Leu Leu Glu 
65                  70                  75                  80  
Pro Leu Gly Asp Ala Leu Pro Gly Arg Val Leu Val Asn Leu Thr Ser 
                85                  90                  95      
Gly Thr Ser Arg Glu Ala Arg Glu Asn Ala Glu His Thr Ala Ala Gln 
            100                 105                 110         
Gly Ala Thr Tyr Leu Asp Gly Val Ile Leu Thr Ile Pro Ser Gly Ile 
        115                 120                 125             
Gly Thr Pro Glu Ala Thr Ile Leu Tyr Ser Gly Pro Arg Ser Thr Phe 
    130                 135                 140                 
Asp Glu His Glu Ser Ala Leu Arg Ala Leu Gly Pro Gly Ala Thr Phe 
145                 150                 155                 160 
Val Gly Glu Asp His Gly Leu Ala Ala Leu Tyr Asp Ala Ala Met Leu 
                165                 170                 175     
Ser Val Met Trp Gly Leu Leu Asn Gly Phe Leu His Gly Thr Ala Leu 
            180                 185                 190         
Leu Gly Thr Ala Gly Val Ser Ala Thr Ser Val Val Pro Met Leu Gly 
        195                 200                 205             
Gln Gly Leu Glu Ala Val Thr Gly Trp Ile Ala Arg Tyr Ala Asp Gln 
    210                 215                 220                 
Ile Asp Ala Gly Val Tyr Pro Ala Asp Asp Ala Thr Ile Asp Thr Gln 
225                 230                 235                 240 
Arg Ala Ala Met Gln His Leu Val Glu Glu Ser Arg Ala Ala Gly Ile 
                245                 250                 255     
Asn Gly Glu Leu Pro Ala Phe Val Thr Lys Leu Ala Asp Arg Ala Val 
            260                 265                 270         
Ala Glu Gly Arg Gly Gly Asp Ser Tyr Ala Ala Leu Ile Glu Gln Phe 
        275                 280                 285             
Arg Lys Ser Ala                                                 
    290                                                         

 
<210> 71
<211> 879
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Imine reductase variant from Pseudonocardia spinosispora  
 
<400> 71
atgagtgatt cccccgtacc tgtgactgtt ctgggtcttg gtctgatggg tcaggcctta 60
gcgggagcgt ttctggcagc cggtcaccct acgacggttt ggaatcgcag cgcagctaaa 120
gctgatgaac tggttggtcg cggtgcaacc ctggccgcgt ctcccgcggc tgccgtcgca 180
gcgggtagtc tgatcgttgt ttgcgtcaca gatcatcgtg cggtgcgcga actgctggaa 240
ccacttggcg atgcactgcc aggtcgcgtt ctggtcaacc tgacgagcgg tacgtcgcgt 300
gaagcccgtg agaacgcaga acatactgcg gctcagggag ccacctacct ggatggtgtg 360
attctgacca ttccaagcgg cattggcacc ccagaagcta ccattctgta ttcgggaccg 420
cgcagtacct tcgatgaaca tgaaagcgca ctgcgcgcat taggtccggg ggcgaccttt 480
gtaggggaag atcacggact ggctgcactg tatgatgcag caatgctgag cgtcatgtgg 540
ggcctgctga atggtttcct gcatggaacc gcactgctgg gaaccgcggg cgtttcggca 600
acctctgtgg ttccgatgct gggtcaaggt atcggtgcgg tgaccggttg gattgcgcgc 660
tacgcagatc agattgatgc cggtgtctat ccagcagacg acgccacgat tgatacccag 720
cgtgctgcaa tgcagcacct ggtcgaggag agccgtgcgg cgggcatcaa tggcgaactt 780
ccggcgtttg tgaccaaact ggccgatcgc gcagtggcag aaggtcgtgg tggtgactct 840
tacgctgcac tgattgaaca gtttcgcaag tcagcttaa                        879

 
<210> 72
<211> 292
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Imine reductase variant from Pseudonocardia spinosispora  
 
<400> 72
Met Ser Asp Ser Pro Val Pro Val Thr Val Leu Gly Leu Gly Leu Met 
1               5                   10                  15      
Gly Gln Ala Leu Ala Gly Ala Phe Leu Ala Ala Gly His Pro Thr Thr 
            20                  25                  30          
Val Trp Asn Arg Ser Ala Ala Lys Ala Asp Glu Leu Val Gly Arg Gly 
        35                  40                  45              
Ala Thr Leu Ala Ala Ser Pro Ala Ala Ala Val Ala Ala Gly Ser Leu 
    50                  55                  60                  
Ile Val Val Cys Val Thr Asp His Arg Ala Val Arg Glu Leu Leu Glu 
65                  70                  75                  80  
Pro Leu Gly Asp Ala Leu Pro Gly Arg Val Leu Val Asn Leu Thr Ser 
                85                  90                  95      
Gly Thr Ser Arg Glu Ala Arg Glu Asn Ala Glu His Thr Ala Ala Gln 
            100                 105                 110         
Gly Ala Thr Tyr Leu Asp Gly Val Ile Leu Thr Ile Pro Ser Gly Ile 
        115                 120                 125             
Gly Thr Pro Glu Ala Thr Ile Leu Tyr Ser Gly Pro Arg Ser Thr Phe 
    130                 135                 140                 
Asp Glu His Glu Ser Ala Leu Arg Ala Leu Gly Pro Gly Ala Thr Phe 
145                 150                 155                 160 
Val Gly Glu Asp His Gly Leu Ala Ala Leu Tyr Asp Ala Ala Met Leu 
                165                 170                 175     
Ser Val Met Trp Gly Leu Leu Asn Gly Phe Leu His Gly Thr Ala Leu 
            180                 185                 190         
Leu Gly Thr Ala Gly Val Ser Ala Thr Ser Val Val Pro Met Leu Gly 
        195                 200                 205             
Gln Gly Ile Gly Ala Val Thr Gly Trp Ile Ala Arg Tyr Ala Asp Gln 
    210                 215                 220                 
Ile Asp Ala Gly Val Tyr Pro Ala Asp Asp Ala Thr Ile Asp Thr Gln 
225                 230                 235                 240 
Arg Ala Ala Met Gln His Leu Val Glu Glu Ser Arg Ala Ala Gly Ile 
                245                 250                 255     
Asn Gly Glu Leu Pro Ala Phe Val Thr Lys Leu Ala Asp Arg Ala Val 
            260                 265                 270         
Ala Glu Gly Arg Gly Gly Asp Ser Tyr Ala Ala Leu Ile Glu Gln Phe 
        275                 280                 285             
Arg Lys Ser Ala                                                 
    290                                                         

 
<210> 73
<211> 879
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Imine reductase variant from Pseudonocardia spinosispora  
 
<400> 73
atgagtgatt cccccgtacc tgtgactgtt ctgggtcttg gtctgatggg tcaggcctta 60
gcgggagcgt ttctggcagc cggtcaccct acgacggttt ggaatcgcag cgcagctaaa 120
gctgatgaac tggttggtcg cggtgcaacc ctggccgcgt ctcccgcggc tgccgtcgca 180
gcgggtagtc tgatcgttgt ttgcgtcaca gatcatcgtg cggtgcgcga actgctggaa 240
ccacttggcg atgcactgcc aggtcgcgtt ctggtcaacc tgacgagcgg tacgtcgcgt 300
gaagcccgtg agaacgcaga acatactgcg gctcagggag ccacctacct ggatggtgtg 360
attctgacca ttccaagcgg cattggcacc ccagaagcta ccattctgta ttcgggaccg 420
cgcagtacct tcgatgaaca tgaaagcgca ctgcgcgcat taggtccggg ggcgaccttt 480
gtaggggaag atcacggact ggctgcactg tatgatgcag caatgctgag cgtcatgtgg 540
ggcctgctga atggtttcct gcatggaacc gcactgctgg gaaccgcggg cgtttcggca 600
acctctgtgg ttccgatgct gggtcaaggt atcgaagcga tgaccggttg gattgcgcgc 660
tacgcagatc agattgatgc cggtgtctat ccagcagacg acgccacgat tgatacccag 720
cgtgctgcaa tgcagcacct ggtcgaggag agccgtgcgg cgggcatcaa tggcgaactt 780
ccggcgtttg tgaccaaact ggccgatcgc gcagtggcag aaggtcgtgg tggtgactct 840
tacgctgcac tgattgaaca gtttcgcaag tcagcttaa                        879

 
<210> 74
<211> 292
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Imine reductase variant from Pseudonocardia spinosispora  
 
<400> 74
Met Ser Asp Ser Pro Val Pro Val Thr Val Leu Gly Leu Gly Leu Met 
1               5                   10                  15      
Gly Gln Ala Leu Ala Gly Ala Phe Leu Ala Ala Gly His Pro Thr Thr 
            20                  25                  30          
Val Trp Asn Arg Ser Ala Ala Lys Ala Asp Glu Leu Val Gly Arg Gly 
        35                  40                  45              
Ala Thr Leu Ala Ala Ser Pro Ala Ala Ala Val Ala Ala Gly Ser Leu 
    50                  55                  60                  
Ile Val Val Cys Val Thr Asp His Arg Ala Val Arg Glu Leu Leu Glu 
65                  70                  75                  80  
Pro Leu Gly Asp Ala Leu Pro Gly Arg Val Leu Val Asn Leu Thr Ser 
                85                  90                  95      
Gly Thr Ser Arg Glu Ala Arg Glu Asn Ala Glu His Thr Ala Ala Gln 
            100                 105                 110         
Gly Ala Thr Tyr Leu Asp Gly Val Ile Leu Thr Ile Pro Ser Gly Ile 
        115                 120                 125             
Gly Thr Pro Glu Ala Thr Ile Leu Tyr Ser Gly Pro Arg Ser Thr Phe 
    130                 135                 140                 
Asp Glu His Glu Ser Ala Leu Arg Ala Leu Gly Pro Gly Ala Thr Phe 
145                 150                 155                 160 
Val Gly Glu Asp His Gly Leu Ala Ala Leu Tyr Asp Ala Ala Met Leu 
                165                 170                 175     
Ser Val Met Trp Gly Leu Leu Asn Gly Phe Leu His Gly Thr Ala Leu 
            180                 185                 190         
Leu Gly Thr Ala Gly Val Ser Ala Thr Ser Val Val Pro Met Leu Gly 
        195                 200                 205             
Gln Gly Ile Glu Ala Met Thr Gly Trp Ile Ala Arg Tyr Ala Asp Gln 
    210                 215                 220                 
Ile Asp Ala Gly Val Tyr Pro Ala Asp Asp Ala Thr Ile Asp Thr Gln 
225                 230                 235                 240 
Arg Ala Ala Met Gln His Leu Val Glu Glu Ser Arg Ala Ala Gly Ile 
                245                 250                 255     
Asn Gly Glu Leu Pro Ala Phe Val Thr Lys Leu Ala Asp Arg Ala Val 
            260                 265                 270         
Ala Glu Gly Arg Gly Gly Asp Ser Tyr Ala Ala Leu Ile Glu Gln Phe 
        275                 280                 285             
Arg Lys Ser Ala                                                 
    290                                                         

	
<210> 75
<211> 879
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Imine reductase variant from Pseudonocardia spinosispora
 
<400> 75
atgagtgatt cacctgtgcc cgtgacagtt ctgggtcttg gtctgatggg tcaggcctta 60
gcgggagcgt ttctggcagc cggtcaccct acgacggttt ggaatcgcag cgcagctaaa 120
gctgatgaac tggttggtcg cggtgcaacc ctggccgcgt ctcccgcggc tgccgtcgca 180
gcgggtagtc tgatcgttgt ttgcgtcaca gatcatcgtg cggtgcgcga actgctggaa 240
ccacttggcg atgcactgcc aggtcgcgtt ctggtcaacc tgacgagcgg tacgtcgcgt 300
gaagcccgtg agaacgcaga acatactgcg gctcagggag ccacctacct ggatggtgtg 360
attctgggtg gcccaagcgg cattggcacc ccagaagcta ccattctgta ttcgggaccg 420
cgcagtacct tcgatgaaca tgaaagcgca ctgcgcgcat taggtccggg ggcgaccttt 480
gtaggggaag atcacggact ggctaatctg gttgatcatg caatgctgag cgtcatgtgg 540
ggcctgctga atggtttcct gcatggaacc gcactgctgg gaaccgcggg cgtttcggca 600
acctctgtgg ttccgatgct gggtcaaggt atcgaagtgg tgaccggttg gattgcgcgc 660
tacgcagatc agattgatgc cggtgtctat ccagcagacg acaagacgat tgatacccag 720
cgtgctgcaa tgcagcacct ggtcgaggag agccgtgcgg cgggcatcaa tggcgaactt 780
ccggcgtttg tgaccaaact ggccgatcgc gcagtggcag aaggtcgtgg tggtgactct 840
tacgctgcac tgattgaaca gtttcgcaag tcagcttaa                        879

 
<210> 76
<211> 292
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Imine reductase variant from Pseudonocardia spinosispora
 
<400> 76
Met Ser Asp Ser Pro Val Pro Val Thr Val Leu Gly Leu Gly Leu Met 
1               5                   10                  15      
Gly Gln Ala Leu Ala Gly Ala Phe Leu Ala Ala Gly His Pro Thr Thr 
            20                  25                  30          
Val Trp Asn Arg Ser Ala Ala Lys Ala Asp Glu Leu Val Gly Arg Gly 
        35                  40                  45              
Ala Thr Leu Ala Ala Ser Pro Ala Ala Ala Val Ala Ala Gly Ser Leu 
    50                  55                  60                  
Ile Val Val Cys Val Thr Asp His Arg Ala Val Arg Glu Leu Leu Glu 
65                  70                  75                  80  
Pro Leu Gly Asp Ala Leu Pro Gly Arg Val Leu Val Asn Leu Thr Ser 
                85                  90                  95      
Gly Thr Ser Arg Glu Ala Arg Glu Asn Ala Glu His Thr Ala Ala Gln 
            100                 105                 110         
Gly Ala Thr Tyr Leu Asp Gly Val Ile Leu Gly Gly Pro Ser Gly Ile 
        115                 120                 125             
Gly Thr Pro Glu Ala Thr Ile Leu Tyr Ser Gly Pro Arg Ser Thr Phe 
    130                 135                 140                 
Asp Glu His Glu Ser Ala Leu Arg Ala Leu Gly Pro Gly Ala Thr Phe 
145                 150                 155                 160 
Val Gly Glu Asp His Gly Leu Ala Asn Leu Val Asp His Ala Met Leu 
                165                 170                 175     
Ser Val Met Trp Gly Leu Leu Asn Gly Phe Leu His Gly Thr Ala Leu 
            180                 185                 190         
Leu Gly Thr Ala Gly Val Ser Ala Thr Ser Val Val Pro Met Leu Gly 
        195                 200                 205             
Gln Gly Ile Glu Val Val Thr Gly Trp Ile Ala Arg Tyr Ala Asp Gln 
    210                 215                 220                 
Ile Asp Ala Gly Val Tyr Pro Ala Asp Asp Lys Thr Ile Asp Thr Gln 
225                 230                 235                 240 
Arg Ala Ala Met Gln His Leu Val Glu Glu Ser Arg Ala Ala Gly Ile 
                245                 250                 255     
Asn Gly Glu Leu Pro Ala Phe Val Thr Lys Leu Ala Asp Arg Ala Val 
            260                 265                 270         
Ala Glu Gly Arg Gly Gly Asp Ser Tyr Ala Ala Leu Ile Glu Gln Phe 
        275                 280                 285             
Arg Lys Ser Ala                                                 
    290                                                         

 
<210> 77
<211> 879
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Imine reductase variant from Pseudonocardia spinosispora
 
<400> 77
atgagtgatt cacctgtgcc cgtgacagtt ctgggtcttg gtctgatggg tcaggcctta 60
gcgggagcgt ttctggcagc cggtcaccct acgacggttt ggaatcgcag cgcagctaaa 120
gctgatgaac tggttggtcg cggtgcaacc ctggccgcgt ctcccgcggc tgccgtcgca 180
gcgggtagtc tgatcgttgt ttgcgtcaca gatcatcgtg cggtgcgcga actgctggaa 240
ccacttggcg atgcactgcc aggtcgcgtt ctggtcaacc tgacgagcgg tacgtcgcgt 300
gaagcccgtg agaacgcaga acatactgcg gctcagggag ccacctacct ggatggtgtg 360
attctgacgg gcccaagcgg cattggcacc ccagaagcta ccattctgta ttcgggaccg 420
cgcagtacct tcgatgaaca tgaaagcgca ctgcgcgcat taggtccggg ggcgaccttt 480
gtaggggaag atcacggact ggctaatctg tacgatattg caatgctgag cgtcatgtgg 540
ggcctgctga atggtttcct gcatggaacc gcactgctgg gaaccgcggg cgtttcggca 600
acctctgtgg ttccgatgct gggtcaaggt atcggagtgg tgaccggttg gattgcgcgc 660
tacgcagatc agattgatgc cggtgtctat ccagcagacg acgcgacgat tgatacccag 720
cgtgctgcaa tgcagcacct ggtcgaggag agccgtgcgg cgggcatcaa tggcgaactt 780
ccggcgtttg tgaccaaact ggccgatcgc gcagtggcag aaggtcgtgg tggtgactct 840
tacgctgcac tgattgaaca gtttcgcaag tcagcttaa                        879

 
<210> 78
<211> 292
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Imine reductase variant from Pseudonocardia spinosispora
 
<400> 78
Met Ser Asp Ser Pro Val Pro Val Thr Val Leu Gly Leu Gly Leu Met 
1               5                   10                  15      
Gly Gln Ala Leu Ala Gly Ala Phe Leu Ala Ala Gly His Pro Thr Thr 
            20                  25                  30          
Val Trp Asn Arg Ser Ala Ala Lys Ala Asp Glu Leu Val Gly Arg Gly 
        35                  40                  45              
Ala Thr Leu Ala Ala Ser Pro Ala Ala Ala Val Ala Ala Gly Ser Leu 
    50                  55                  60                  
Ile Val Val Cys Val Thr Asp His Arg Ala Val Arg Glu Leu Leu Glu 
65                  70                  75                  80  
Pro Leu Gly Asp Ala Leu Pro Gly Arg Val Leu Val Asn Leu Thr Ser 
                85                  90                  95      
Gly Thr Ser Arg Glu Ala Arg Glu Asn Ala Glu His Thr Ala Ala Gln 
            100                 105                 110         
Gly Ala Thr Tyr Leu Asp Gly Val Ile Leu Thr Gly Pro Ser Gly Ile 
        115                 120                 125             
Gly Thr Pro Glu Ala Thr Ile Leu Tyr Ser Gly Pro Arg Ser Thr Phe 
    130                 135                 140                 
Asp Glu His Glu Ser Ala Leu Arg Ala Leu Gly Pro Gly Ala Thr Phe 
145                 150                 155                 160 
Val Gly Glu Asp His Gly Leu Ala Asn Leu Tyr Asp Ile Ala Met Leu 
                165                 170                 175     
Ser Val Met Trp Gly Leu Leu Asn Gly Phe Leu His Gly Thr Ala Leu 
            180                 185                 190         
Leu Gly Thr Ala Gly Val Ser Ala Thr Ser Val Val Pro Met Leu Gly 
        195                 200                 205             
Gln Gly Ile Gly Val Val Thr Gly Trp Ile Ala Arg Tyr Ala Asp Gln 
    210                 215                 220                 
Ile Asp Ala Gly Val Tyr Pro Ala Asp Asp Ala Thr Ile Asp Thr Gln 
225                 230                 235                 240 
Arg Ala Ala Met Gln His Leu Val Glu Glu Ser Arg Ala Ala Gly Ile 
                245                 250                 255     
Asn Gly Glu Leu Pro Ala Phe Val Thr Lys Leu Ala Asp Arg Ala Val 
            260                 265                 270         
Ala Glu Gly Arg Gly Gly Asp Ser Tyr Ala Ala Leu Ile Glu Gln Phe 
        275                 280                 285             
Arg Lys Ser Ala                                                 
    290                                                         

 
<210> 79
<211> 879
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Imine reductase variant from Pseudonocardia spinosispora
 
<400> 79
atgagtgatt cacctgtgcc cgtgacagtt ctgggtcttg gtctgatggg tcaggcctta 60
gcgggagcgt ttctggcagc cggtcaccct acgacggttt ggaatcgcag cgcagctaaa 120
gctgatgaac tggttggtcg cggtgcaacc ctggccgcgt ctcccgcggc tgccgtcgca 180
gcgggtagtc tgatcgttgt ttgcgtcaca gatcatcgtg cggtgcgcga actgctggaa 240
ccacttggcg atgcactgcc aggtcgcgtt ctggtcaacc tgacgagcgg tacgtcgcgt 300
gaagcccgtg agaacgcaga acatactgcg gctcagggag ccacctacct ggatggtgtg 360
attctgacgg gcccaagcgg cattggcacc ccagaagcta ccattctgta ttcgggaccg 420
cgcagtacct tcgatgaaca tgaaagcgca ctgcgcgcat taggtccggg ggcgaccttt 480
gtaggggaag atcacggact ggctaatctg tacgatgccg caggcctgag cgtcatgtgg 540
ggcctgctga atggtttcct gcatggaacc gcactgctgg gaaccgcggg cgtttcggca 600
acctctgtgg ttccgatgct gggtcaaggt atctgcgtgg tgaccggttg gattgcgcgc 660
tacgcagatc agattgatgc cggtgtctat ccagcagacg acaagacgat tgatacccag 720
cgtgctgcaa tgcagcacct ggtcgaggag agccgtgcgg cgggcatcaa tggcgaactt 780
ccggcgtttg tgaccaaact ggccgatcgc gcagtggcag aaggtcgtgg tggtgactct 840
tacgctgcac tgattgaaca gtttcgcaag tcagcttaa                        879

 
<210> 80
<211> 292
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Imine reductase variant from Pseudonocardia spinosispora
 
<400> 80
Met Ser Asp Ser Pro Val Pro Val Thr Val Leu Gly Leu Gly Leu Met 
1               5                   10                  15      
Gly Gln Ala Leu Ala Gly Ala Phe Leu Ala Ala Gly His Pro Thr Thr 
            20                  25                  30          
Val Trp Asn Arg Ser Ala Ala Lys Ala Asp Glu Leu Val Gly Arg Gly 
        35                  40                  45              
Ala Thr Leu Ala Ala Ser Pro Ala Ala Ala Val Ala Ala Gly Ser Leu 
    50                  55                  60                  
Ile Val Val Cys Val Thr Asp His Arg Ala Val Arg Glu Leu Leu Glu 
65                  70                  75                  80  
Pro Leu Gly Asp Ala Leu Pro Gly Arg Val Leu Val Asn Leu Thr Ser 
                85                  90                  95      
Gly Thr Ser Arg Glu Ala Arg Glu Asn Ala Glu His Thr Ala Ala Gln 
            100                 105                 110         
Gly Ala Thr Tyr Leu Asp Gly Val Ile Leu Thr Gly Pro Ser Gly Ile 
        115                 120                 125             
Gly Thr Pro Glu Ala Thr Ile Leu Tyr Ser Gly Pro Arg Ser Thr Phe 
    130                 135                 140                 
Asp Glu His Glu Ser Ala Leu Arg Ala Leu Gly Pro Gly Ala Thr Phe 
145                 150                 155                 160 
Val Gly Glu Asp His Gly Leu Ala Asn Leu Tyr Asp Ala Ala Gly Leu 
                165                 170                 175     
Ser Val Met Trp Gly Leu Leu Asn Gly Phe Leu His Gly Thr Ala Leu 
            180                 185                 190         
Leu Gly Thr Ala Gly Val Ser Ala Thr Ser Val Val Pro Met Leu Gly 
        195                 200                 205             
Gln Gly Ile Cys Val Val Thr Gly Trp Ile Ala Arg Tyr Ala Asp Gln 
    210                 215                 220                 
Ile Asp Ala Gly Val Tyr Pro Ala Asp Asp Lys Thr Ile Asp Thr Gln 
225                 230                 235                 240 
Arg Ala Ala Met Gln His Leu Val Glu Glu Ser Arg Ala Ala Gly Ile 
                245                 250                 255     
Asn Gly Glu Leu Pro Ala Phe Val Thr Lys Leu Ala Asp Arg Ala Val 
            260                 265                 270         
Ala Glu Gly Arg Gly Gly Asp Ser Tyr Ala Ala Leu Ile Glu Gln Phe 
        275                 280                 285             
Arg Lys Ser Ala                                                 
    290                                                         

 
<210> 81
<211> 879
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Imine reductase variant from Pseudonocardia spinosispora
 
<400> 81
atgagtgatt cacctgtgcc cgtgacagtt ctgggtcttg gtctgatggg tcaggcctta 60
gcgggagcgt ttctggcagc cggtcaccct acgacggttt ggaatcgcag cgcagctaaa 120
gctgatgaac tggttggtcg cggtgcaacc ctggccgcgt ctcccgcggc tgccgtcgca 180
gcgggtagtc tgatcgttgt ttgcgtcaca gatcatcgtg cggtgcgcga actgctggaa 240
ccacttggcg atgcactgcc aggtcgcgtt ctggtcaacc tgacgagcgg tacgtcgcgt 300
gaagcccgtg agaacgcaga acatactgcg gctcagggag ccacctacct ggatggtgtg 360
attctgacga ttccaagcgg cattggcacc ccagaagcta ccattctgta ttcgggaccg 420
cgcagtacct tcgatgaaca tgaaagcgca ctgcgcgcat taggtccggg ggcgaccttt 480
gtaggggaag atcacggact ggctgccctg gttgatattg caatgctgag cgtcatgtgg 540
ggcctgctga atggtttcct gcatggaacc gcactgctgg gaaccgcggg cgtttcggca 600
acctctgtgg ttccgatgct gggtcaaggt atcggagtgg tgaccggttg gattgcgcgc 660
tacgcagatc agattgatgc cggtgtctat ccagcagacg acaagacgat tgatacccag 720
cgtgctgcaa tgcagcacct ggtcgaggag agccgtgcgg cgggcatcaa tggcgaactt 780
ccggcgtttg tgaccaaact ggccgatcgc gcagtggcag aaggtcgtgg tggtgactct 840
tacgctgcac tgattgaaca gtttcgcaag tcagcttaa                        879

 
<210> 82
<211> 292
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Imine reductase variant from Pseudonocardia spinosispora
 
<400> 82
Met Ser Asp Ser Pro Val Pro Val Thr Val Leu Gly Leu Gly Leu Met 
1               5                   10                  15      
Gly Gln Ala Leu Ala Gly Ala Phe Leu Ala Ala Gly His Pro Thr Thr 
            20                  25                  30          
Val Trp Asn Arg Ser Ala Ala Lys Ala Asp Glu Leu Val Gly Arg Gly 
        35                  40                  45              
Ala Thr Leu Ala Ala Ser Pro Ala Ala Ala Val Ala Ala Gly Ser Leu 
    50                  55                  60                  
Ile Val Val Cys Val Thr Asp His Arg Ala Val Arg Glu Leu Leu Glu 
65                  70                  75                  80  
Pro Leu Gly Asp Ala Leu Pro Gly Arg Val Leu Val Asn Leu Thr Ser 
                85                  90                  95      
Gly Thr Ser Arg Glu Ala Arg Glu Asn Ala Glu His Thr Ala Ala Gln 
            100                 105                 110         
Gly Ala Thr Tyr Leu Asp Gly Val Ile Leu Thr Ile Pro Ser Gly Ile 
        115                 120                 125             
Gly Thr Pro Glu Ala Thr Ile Leu Tyr Ser Gly Pro Arg Ser Thr Phe 
    130                 135                 140                 
Asp Glu His Glu Ser Ala Leu Arg Ala Leu Gly Pro Gly Ala Thr Phe 
145                 150                 155                 160 
Val Gly Glu Asp His Gly Leu Ala Ala Leu Val Asp Ile Ala Met Leu 
                165                 170                 175     
Ser Val Met Trp Gly Leu Leu Asn Gly Phe Leu His Gly Thr Ala Leu 
            180                 185                 190         
Leu Gly Thr Ala Gly Val Ser Ala Thr Ser Val Val Pro Met Leu Gly 
        195                 200                 205             
Gln Gly Ile Gly Val Val Thr Gly Trp Ile Ala Arg Tyr Ala Asp Gln 
    210                 215                 220                 
Ile Asp Ala Gly Val Tyr Pro Ala Asp Asp Lys Thr Ile Asp Thr Gln 
225                 230                 235                 240 
Arg Ala Ala Met Gln His Leu Val Glu Glu Ser Arg Ala Ala Gly Ile 
                245                 250                 255     
Asn Gly Glu Leu Pro Ala Phe Val Thr Lys Leu Ala Asp Arg Ala Val 
            260                 265                 270         
Ala Glu Gly Arg Gly Gly Asp Ser Tyr Ala Ala Leu Ile Glu Gln Phe 
        275                 280                 285             
Arg Lys Ser Ala                                                 
    290                                                         

 
<210> 83
<211> 879
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Imine reductase variant from Pseudonocardia spinosispora
 
<400> 83
atgagtgatt cacctgtgcc cgtgacagtt ctgggtcttg gtctgatggg tcaggcctta 60
gcgggagcgt ttctggcagc cggtcaccct acgacggttt ggaatcgcag cgcagctaaa 120
gctgatgaac tggttggtcg cggtgcaacc ctggccgcgt ctcccgcggc tgccgtcgca 180
gcgggtagtc tgatcgttgt ttgcgtcaca gatcatcgtg cggtgcgcga actgctggaa 240
ccacttggcg atgcactgcc aggtcgcgtt ctggtcaacc tgacgagcgg tacgtcgcgt 300
gaagcccgtg agaacgcaga acatactgcg gctcagggag ccacctacct ggatggtgtg 360
attctgacga ttccaagcgg cattggcacc ccagaagcta ccattctgta ttcgggaccg 420
cgcagtacct tcgatgaaca tgaaagcgca ctgcgcgcat taggtccggg ggcgaccttt 480
gtaggggaag atcacggact ggctaatctg tacgatgcag caatgctgag cgtcatgtgg 540
ggcctgctga atggtttcct gcatggaacc gcactgctgg gaaccgcggg cgtttcggca 600
acctctgtgg ttccgatgct gggtcaaggt atcgaagcgg tgaccggttg gattgcgcgc 660
tacgcagatc agattgatgc cggtgtctat ccagcagacg acaagacgat tgatacccag 720
cgtgctgcaa tgcagcacct ggtcgaggag agccgtgcgg cgggcatcaa tggcgaactt 780
ccggcgtttg tgaccaaact ggccgatcgc gcagtggcag aaggtcgtgg tggtgactct 840
tacgctgcac tgattgaaca gtttcgcaag tcagcttaa                        879

 
<210> 84
<211> 292
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Imine reductase variant from Pseudonocardia spinosispora
 
<400> 84
Met Ser Asp Ser Pro Val Pro Val Thr Val Leu Gly Leu Gly Leu Met 
1               5                   10                  15      
Gly Gln Ala Leu Ala Gly Ala Phe Leu Ala Ala Gly His Pro Thr Thr 
            20                  25                  30          
Val Trp Asn Arg Ser Ala Ala Lys Ala Asp Glu Leu Val Gly Arg Gly 
        35                  40                  45              
Ala Thr Leu Ala Ala Ser Pro Ala Ala Ala Val Ala Ala Gly Ser Leu 
    50                  55                  60                  
Ile Val Val Cys Val Thr Asp His Arg Ala Val Arg Glu Leu Leu Glu 
65                  70                  75                  80  
Pro Leu Gly Asp Ala Leu Pro Gly Arg Val Leu Val Asn Leu Thr Ser 
                85                  90                  95      
Gly Thr Ser Arg Glu Ala Arg Glu Asn Ala Glu His Thr Ala Ala Gln 
            100                 105                 110         
Gly Ala Thr Tyr Leu Asp Gly Val Ile Leu Thr Ile Pro Ser Gly Ile 
        115                 120                 125             
Gly Thr Pro Glu Ala Thr Ile Leu Tyr Ser Gly Pro Arg Ser Thr Phe 
    130                 135                 140                 
Asp Glu His Glu Ser Ala Leu Arg Ala Leu Gly Pro Gly Ala Thr Phe 
145                 150                 155                 160 
Val Gly Glu Asp His Gly Leu Ala Asn Leu Tyr Asp Ala Ala Met Leu 
                165                 170                 175     
Ser Val Met Trp Gly Leu Leu Asn Gly Phe Leu His Gly Thr Ala Leu 
            180                 185                 190         
Leu Gly Thr Ala Gly Val Ser Ala Thr Ser Val Val Pro Met Leu Gly 
        195                 200                 205             
Gln Gly Ile Glu Ala Val Thr Gly Trp Ile Ala Arg Tyr Ala Asp Gln 
    210                 215                 220                 
Ile Asp Ala Gly Val Tyr Pro Ala Asp Asp Lys Thr Ile Asp Thr Gln 
225                 230                 235                 240 
Arg Ala Ala Met Gln His Leu Val Glu Glu Ser Arg Ala Ala Gly Ile 
                245                 250                 255     
Asn Gly Glu Leu Pro Ala Phe Val Thr Lys Leu Ala Asp Arg Ala Val 
            260                 265                 270         
Ala Glu Gly Arg Gly Gly Asp Ser Tyr Ala Ala Leu Ile Glu Gln Phe 
        275                 280                 285             
Arg Lys Ser Ala                                                 
    290                                                         

 
<210> 85
<211> 879
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Imine reductase variant from Pseudonocardia spinosispora
 
<400> 85
atgagtgatt cacctgtgcc cgtgacagtt ctgggtcttg gtctgatggg tcaggcctta 60
gcgggagcgt ttctggcagc cggtcaccct acgacggttt ggaatcgcag cgcagctaaa 120
gctgatgaac tggttggtcg cggtgcaacc ctggccgcgt ctcccgcggc tgccgtcgca 180
gcgggtagtc tgatcgttgt ttgcgtcaca gatcatcgtg cggtgcgcga actgctggaa 240
ccacttggcg atgcactgcc aggtcgcgtt ctggtcaacc tgacgagcgg tacgtcgcgt 300
gaagcccgtg agaacgcaga acatactgcg gctcagggag ccacctacct ggatggtgtg 360
attctgggta ttccaagcgg cattggcacc ccagaagcta ccattctgta ttcgggaccg 420
cgcagtacct tcgatgaaca tgaaagcgca ctgcgcgcat taggtccggg ggcgaccttt 480
gtaggggaag atcacggact ggctaatctg tacgatcatg cacacctgag cgtcatgtgg 540
ggcctgctga atggtttcct gcatggaacc gcactgctgg gaaccgcggg cgtttcggca 600
acctctgtgg ttccgatgct gggtcaaggt atcgaagtgg tgaccggttg gattgcgcgc 660
tacgcagatc agattgatgc cggtgtctat ccagcagacg acaagacgat tgatacccag 720
cgtgctgcaa tgcagcacct ggtcgaggag agccgtgcgg cgggcatcaa tggcgaactt 780
ccggcgtttg tgaccaaact ggccgatcgc gcagtggcag aaggtcgtgg tggtgactct 840
tacgctgcac tgattgaaca gtttcgcaag tcagcttaa                        879

 
<210> 86
<211> 292
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Imine reductase variant from Pseudonocardia spinosispora
 
<400> 86
Met Ser Asp Ser Pro Val Pro Val Thr Val Leu Gly Leu Gly Leu Met 
1               5                   10                  15      
Gly Gln Ala Leu Ala Gly Ala Phe Leu Ala Ala Gly His Pro Thr Thr 
            20                  25                  30          
Val Trp Asn Arg Ser Ala Ala Lys Ala Asp Glu Leu Val Gly Arg Gly 
        35                  40                  45              
Ala Thr Leu Ala Ala Ser Pro Ala Ala Ala Val Ala Ala Gly Ser Leu 
    50                  55                  60                  
Ile Val Val Cys Val Thr Asp His Arg Ala Val Arg Glu Leu Leu Glu 
65                  70                  75                  80  
Pro Leu Gly Asp Ala Leu Pro Gly Arg Val Leu Val Asn Leu Thr Ser 
                85                  90                  95      
Gly Thr Ser Arg Glu Ala Arg Glu Asn Ala Glu His Thr Ala Ala Gln 
            100                 105                 110         
Gly Ala Thr Tyr Leu Asp Gly Val Ile Leu Gly Ile Pro Ser Gly Ile 
        115                 120                 125             
Gly Thr Pro Glu Ala Thr Ile Leu Tyr Ser Gly Pro Arg Ser Thr Phe 
    130                 135                 140                 
Asp Glu His Glu Ser Ala Leu Arg Ala Leu Gly Pro Gly Ala Thr Phe 
145                 150                 155                 160 
Val Gly Glu Asp His Gly Leu Ala Asn Leu Tyr Asp His Ala His Leu 
                165                 170                 175     
Ser Val Met Trp Gly Leu Leu Asn Gly Phe Leu His Gly Thr Ala Leu 
            180                 185                 190         
Leu Gly Thr Ala Gly Val Ser Ala Thr Ser Val Val Pro Met Leu Gly 
        195                 200                 205             
Gln Gly Ile Glu Val Val Thr Gly Trp Ile Ala Arg Tyr Ala Asp Gln 
    210                 215                 220                 
Ile Asp Ala Gly Val Tyr Pro Ala Asp Asp Lys Thr Ile Asp Thr Gln 
225                 230                 235                 240 
Arg Ala Ala Met Gln His Leu Val Glu Glu Ser Arg Ala Ala Gly Ile 
                245                 250                 255     
Asn Gly Glu Leu Pro Ala Phe Val Thr Lys Leu Ala Asp Arg Ala Val 
            260                 265                 270         
Ala Glu Gly Arg Gly Gly Asp Ser Tyr Ala Ala Leu Ile Glu Gln Phe 
        275                 280                 285             
Arg Lys Ser Ala                                                 
    290                                                         

 
<210> 87
<211> 879
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Imine reductase variant from Pseudonocardia spinosispora
 
<400> 87
atgagtgatt cacctgtgcc cgtgacagtt ctgggtcttg gtctgatggg tcaggcctta 60
gcgggagcgt ttctggcagc cggtcaccct acgacggttt ggaatcgcag cgcagctaaa 120
gctgatgaac tggttggtcg cggtgcaacc ctggccgcgt ctcccgcggc tgccgtcgca 180
gcgggtagtc tgatcgttgt ttgcgtcaca gatcatcgtg cggtgcgcga actgctggaa 240
ccacttggcg atgcactgcc aggtcgcgtt ctggtcaacc tgacgagcgg tacgtcgcgt 300
gaagcccgtg agaacgcaga acatactgcg gctcagggag ccacctacct ggatggtgtg 360
attctgggta ttccaagcgg cattggcacc ccagaagcta ccattctgta ttcgggaccg 420
cgcagtacct tcgatgaaca tgaaagcgca ctgcgcgcat taggtccggg ggcgaccttt 480
gtaggggaag atcacggact ggctaatctg tacgatattg caatgctgag cgtcatgtgg 540
ggcctgctga atggtttcct gcatggaacc gcactgctgg gaaccgcggg cgtttcggca 600
acctctgtgg ttccgatgct gggtcaaggt atcggaactg tgaccggttg gattgcgcgc 660
tacgcagatc agattgatgc cggtgtctat ccagcagacg acgccacgat tgatacccag 720
cgtgctgcaa tgcagcacct ggtcgaggag agccgtgcgg cgggcatcaa tggcgaactt 780
ccggcgtttg tgaccaaact ggccgatcgc gcagtggcag aaggtcgtgg tggtgactct 840
tacgctgcac tgattgaaca gtttcgcaag tcagcttaa                        879

 
<210> 88
<211> 292
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Imine reductase variant from Pseudonocardia spinosispora
 
<400> 88
Met Ser Asp Ser Pro Val Pro Val Thr Val Leu Gly Leu Gly Leu Met 
1               5                   10                  15      
Gly Gln Ala Leu Ala Gly Ala Phe Leu Ala Ala Gly His Pro Thr Thr 
            20                  25                  30          
Val Trp Asn Arg Ser Ala Ala Lys Ala Asp Glu Leu Val Gly Arg Gly 
        35                  40                  45              
Ala Thr Leu Ala Ala Ser Pro Ala Ala Ala Val Ala Ala Gly Ser Leu 
    50                  55                  60                  
Ile Val Val Cys Val Thr Asp His Arg Ala Val Arg Glu Leu Leu Glu 
65                  70                  75                  80  
Pro Leu Gly Asp Ala Leu Pro Gly Arg Val Leu Val Asn Leu Thr Ser 
                85                  90                  95      
Gly Thr Ser Arg Glu Ala Arg Glu Asn Ala Glu His Thr Ala Ala Gln 
            100                 105                 110         
Gly Ala Thr Tyr Leu Asp Gly Val Ile Leu Gly Ile Pro Ser Gly Ile 
        115                 120                 125             
Gly Thr Pro Glu Ala Thr Ile Leu Tyr Ser Gly Pro Arg Ser Thr Phe 
    130                 135                 140                 
Asp Glu His Glu Ser Ala Leu Arg Ala Leu Gly Pro Gly Ala Thr Phe 
145                 150                 155                 160 
Val Gly Glu Asp His Gly Leu Ala Asn Leu Tyr Asp Ile Ala Met Leu 
                165                 170                 175     
Ser Val Met Trp Gly Leu Leu Asn Gly Phe Leu His Gly Thr Ala Leu 
            180                 185                 190         
Leu Gly Thr Ala Gly Val Ser Ala Thr Ser Val Val Pro Met Leu Gly 
        195                 200                 205             
Gln Gly Ile Gly Thr Val Thr Gly Trp Ile Ala Arg Tyr Ala Asp Gln 
    210                 215                 220                 
Ile Asp Ala Gly Val Tyr Pro Ala Asp Asp Ala Thr Ile Asp Thr Gln 
225                 230                 235                 240 
Arg Ala Ala Met Gln His Leu Val Glu Glu Ser Arg Ala Ala Gly Ile 
                245                 250                 255     
Asn Gly Glu Leu Pro Ala Phe Val Thr Lys Leu Ala Asp Arg Ala Val 
            260                 265                 270         
Ala Glu Gly Arg Gly Gly Asp Ser Tyr Ala Ala Leu Ile Glu Gln Phe 
        275                 280                 285             
Arg Lys Ser Ala                                                 
    290                                                         

 
<210> 89
<211> 879
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Imine reductase variant from Pseudonocardia spinosispora
 
<400> 89
atgagtgatt cacctgtgcc cgtgacagtt ctgggtcttg gtctgatggg tcaggcctta 60
gcgggagcgt ttctggcagc cggtcaccct acgacggttt ggaatcgcag cgcagctaaa 120
gctgatgaac tggttggtcg cggtgcaacc ctggccgcgt ctcccgcggc tgccgtcgca 180
gcgggtagtc tgatcgttgt ttgcgtcaca gatcatcgtg cggtgcgcga actgctggaa 240
ccacttggcg atgcactgcc aggtcgcgtt ctggtcaacc tgacgagcgg tacgtcgcgt 300
gaagcccgtg agaacgcaga acatactgcg gctcagggag ccacctacct ggatggtgtg 360
attctgacgg gcccaagcgg cattggcacc ccagaagcta ccattctgta ttcgggaccg 420
cgcagtacct tcgatgaaca tgaaagcgca ctgcgcgcat taggtccggg ggcgaccttt 480
gtaggggaag atcacggact ggctgcactg tatgatgcag caatgctgag cgtcatgtgg 540
ggcctgctga atggtttcct gcatggaacc gcactgctgg gaaccgcggg cgtttcggca 600
acctctgtgg ttccgatgct gggtcaaggt atcggagtgg tgaccggttg gattgcgcgc 660
tacgcagatc agattgatgc cggtgtctat ccagcagacg acaagacgat tgatacccag 720
cgtgctgcaa tgcagcacct ggtcgaggag agccgtgcgg cgggcatcaa tggcgaactt 780
ccggcgtttg tgaccaaact ggccgatcgc gcagtggcag aaggtcgtgg tggtgactct 840
tacgctgcac tgattgaaca gtttcgcaag tcagcttaa                        879

 
<210> 90
<211> 292
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Imine reductase variant from Pseudonocardia spinosispora
 
<400> 90
Met Ser Asp Ser Pro Val Pro Val Thr Val Leu Gly Leu Gly Leu Met 
1               5                   10                  15      
Gly Gln Ala Leu Ala Gly Ala Phe Leu Ala Ala Gly His Pro Thr Thr 
            20                  25                  30          
Val Trp Asn Arg Ser Ala Ala Lys Ala Asp Glu Leu Val Gly Arg Gly 
        35                  40                  45              
Ala Thr Leu Ala Ala Ser Pro Ala Ala Ala Val Ala Ala Gly Ser Leu 
    50                  55                  60                  
Ile Val Val Cys Val Thr Asp His Arg Ala Val Arg Glu Leu Leu Glu 
65                  70                  75                  80  
Pro Leu Gly Asp Ala Leu Pro Gly Arg Val Leu Val Asn Leu Thr Ser 
                85                  90                  95      
Gly Thr Ser Arg Glu Ala Arg Glu Asn Ala Glu His Thr Ala Ala Gln 
            100                 105                 110         
Gly Ala Thr Tyr Leu Asp Gly Val Ile Leu Thr Gly Pro Ser Gly Ile 
        115                 120                 125             
Gly Thr Pro Glu Ala Thr Ile Leu Tyr Ser Gly Pro Arg Ser Thr Phe 
    130                 135                 140                 
Asp Glu His Glu Ser Ala Leu Arg Ala Leu Gly Pro Gly Ala Thr Phe 
145                 150                 155                 160 
Val Gly Glu Asp His Gly Leu Ala Ala Leu Tyr Asp Ala Ala Met Leu 
                165                 170                 175     
Ser Val Met Trp Gly Leu Leu Asn Gly Phe Leu His Gly Thr Ala Leu 
            180                 185                 190         
Leu Gly Thr Ala Gly Val Ser Ala Thr Ser Val Val Pro Met Leu Gly 
        195                 200                 205             
Gln Gly Ile Gly Val Val Thr Gly Trp Ile Ala Arg Tyr Ala Asp Gln 
    210                 215                 220                 
Ile Asp Ala Gly Val Tyr Pro Ala Asp Asp Lys Thr Ile Asp Thr Gln 
225                 230                 235                 240 
Arg Ala Ala Met Gln His Leu Val Glu Glu Ser Arg Ala Ala Gly Ile 
                245                 250                 255     
Asn Gly Glu Leu Pro Ala Phe Val Thr Lys Leu Ala Asp Arg Ala Val 
            260                 265                 270         
Ala Glu Gly Arg Gly Gly Asp Ser Tyr Ala Ala Leu Ile Glu Gln Phe 
        275                 280                 285             
Arg Lys Ser Ala                                                 
    290                                                         

 
<210> 91
<211> 879
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Imine reductase variant from Pseudonocardia spinosispora
 
<400> 91
atgagtgatt cacctgtgcc cgtgacagtt ctgggtcttg gtctgatggg tcaggcctta 60
gcgggagcgt ttctggcagc cggtcaccct acgacggttt ggaatcgcag cgcagctaaa 120
gctgatgaac tggttggtcg cggtgcaacc ctggccgcgt ctcccgcggc tgccgtcgca 180
gcgggtagtc tgatcgttgt ttgcgtcaca gatcatcgtg cggtgcgcga actgctggaa 240
ccacttggcg atgcactgcc aggtcgcgtt ctggtcaacc tgacgagcgg tacgtcgcgt 300
gaagcccgtg agaacgcaga acatactgcg gctcagggag ccacctacct ggatggtgtg 360
attctgggta ttccaagcgg cattggcacc ccagaagcta ccattctgta ttcgggaccg 420
cgcagtacct tcgatgaaca tgaaagcgca ctgcgcgcat taggtccggg ggcgaccttt 480
gtaggggaag atcacggact ggctgccctg tacgatcatg cacacctgag cgtcatgtgg 540
ggcctgctga atggtttcct gcatggaacc gcactgctgg gaaccgcggg cgtttcggca 600
acctctgtgg ttccgatgct gggtcaaggt atctgcgtgg tgaccggttg gattgcgcgc 660
tacgcagatc agattgatgc cggtgtctat ccagcagacg acaagacgat tgatacccag 720
cgtgctgcaa tgcagcacct ggtcgaggag agccgtgcgg cgggcatcaa tggcgaactt 780
ccggcgtttg tgaccaaact ggccgatcgc gcagtggcag aaggtcgtgg tggtgactct 840
tacgctgcac tgattgaaca gtttcgcaag tcagcttaa                        879

 
<210> 92
<211> 292
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Imine reductase variant from Pseudonocardia spinosispora
 
<400> 92
Met Ser Asp Ser Pro Val Pro Val Thr Val Leu Gly Leu Gly Leu Met 
1               5                   10                  15      
Gly Gln Ala Leu Ala Gly Ala Phe Leu Ala Ala Gly His Pro Thr Thr 
            20                  25                  30          
Val Trp Asn Arg Ser Ala Ala Lys Ala Asp Glu Leu Val Gly Arg Gly 
        35                  40                  45              
Ala Thr Leu Ala Ala Ser Pro Ala Ala Ala Val Ala Ala Gly Ser Leu 
    50                  55                  60                  
Ile Val Val Cys Val Thr Asp His Arg Ala Val Arg Glu Leu Leu Glu 
65                  70                  75                  80  
Pro Leu Gly Asp Ala Leu Pro Gly Arg Val Leu Val Asn Leu Thr Ser 
                85                  90                  95      
Gly Thr Ser Arg Glu Ala Arg Glu Asn Ala Glu His Thr Ala Ala Gln 
            100                 105                 110         
Gly Ala Thr Tyr Leu Asp Gly Val Ile Leu Gly Ile Pro Ser Gly Ile 
        115                 120                 125             
Gly Thr Pro Glu Ala Thr Ile Leu Tyr Ser Gly Pro Arg Ser Thr Phe 
    130                 135                 140                 
Asp Glu His Glu Ser Ala Leu Arg Ala Leu Gly Pro Gly Ala Thr Phe 
145                 150                 155                 160 
Val Gly Glu Asp His Gly Leu Ala Ala Leu Tyr Asp His Ala His Leu 
                165                 170                 175     
Ser Val Met Trp Gly Leu Leu Asn Gly Phe Leu His Gly Thr Ala Leu 
            180                 185                 190         
Leu Gly Thr Ala Gly Val Ser Ala Thr Ser Val Val Pro Met Leu Gly 
        195                 200                 205             
Gln Gly Ile Cys Val Val Thr Gly Trp Ile Ala Arg Tyr Ala Asp Gln 
    210                 215                 220                 
Ile Asp Ala Gly Val Tyr Pro Ala Asp Asp Lys Thr Ile Asp Thr Gln 
225                 230                 235                 240 
Arg Ala Ala Met Gln His Leu Val Glu Glu Ser Arg Ala Ala Gly Ile 
                245                 250                 255     
Asn Gly Glu Leu Pro Ala Phe Val Thr Lys Leu Ala Asp Arg Ala Val 
            260                 265                 270         
Ala Glu Gly Arg Gly Gly Asp Ser Tyr Ala Ala Leu Ile Glu Gln Phe 
        275                 280                 285             
Arg Lys Ser Ala                                                 
    290                                                         

 
<210> 93
<211> 879
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Imine reductase variant from Pseudonocardia spinosispora
 
<400> 93
atgagtgatt cacctgtgcc cgtgacagtt ctgggtcttg gtctgatggg tcaggcctta 60
gcgggagcgt ttctggcagc cggtcaccct acgacggttt ggaatcgcag cgcagctaaa 120
gctgatgaac tggttggtcg cggtgcaacc ctggccgcgt ctcccgcggc tgccgtcgca 180
gcgggtagtc tgatcgttgt ttgcgtcaca gatcatcgtg cggtgcgcga actgctggaa 240
ccacttggcg atgcactgcc aggtcgcgtt ctggtcaacc tgacgagcgg tacgtcgcgt 300
gaagcccgtg agaacgcaga acatactgcg gctcagggag ccacctacct ggatggtgtg 360
attctgacga ttccaagcgg cattggcacc ccagaagcta ccattctgta ttcgggaccg 420
cgcagtacct tcgatgaaca tgaaagcgca ctgcgcgcat taggtccggg ggcgaccttt 480
gtaggggaag atcacggact ggctaatctg tacgatgccg caatgctgag cgtcatgtgg 540
ggcctgctga atggtttcct gcatggaacc gcactgctgg gaaccgcggg cgtttcggca 600
acctctgtgg ttccgatgct gggtcaaggt atcggagtgg tgaccggttg gattgcgcgc 660
tacgcagatc agattgatgc cggtgtctat ccagcagacg acgccacgat tgatacccag 720
cgtgctgcaa tgcagcacct ggtcgaggag agccgtgcgg cgggcatcaa tggcgaactt 780
ccggcgtttg tgaccaaact ggccgatcgc gcagtggcag aaggtcgtgg tggtgactct 840
tacgctgcac tgattgaaca gtttcgcaag tcagcttaa                        879

 
<210> 94
<211> 292
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Imine reductase variant from Pseudonocardia spinosispora
 
<400> 94
Met Ser Asp Ser Pro Val Pro Val Thr Val Leu Gly Leu Gly Leu Met 
1               5                   10                  15      
Gly Gln Ala Leu Ala Gly Ala Phe Leu Ala Ala Gly His Pro Thr Thr 
            20                  25                  30          
Val Trp Asn Arg Ser Ala Ala Lys Ala Asp Glu Leu Val Gly Arg Gly 
        35                  40                  45              
Ala Thr Leu Ala Ala Ser Pro Ala Ala Ala Val Ala Ala Gly Ser Leu 
    50                  55                  60                  
Ile Val Val Cys Val Thr Asp His Arg Ala Val Arg Glu Leu Leu Glu 
65                  70                  75                  80  
Pro Leu Gly Asp Ala Leu Pro Gly Arg Val Leu Val Asn Leu Thr Ser 
                85                  90                  95      
Gly Thr Ser Arg Glu Ala Arg Glu Asn Ala Glu His Thr Ala Ala Gln 
            100                 105                 110         
Gly Ala Thr Tyr Leu Asp Gly Val Ile Leu Thr Ile Pro Ser Gly Ile 
        115                 120                 125             
Gly Thr Pro Glu Ala Thr Ile Leu Tyr Ser Gly Pro Arg Ser Thr Phe 
    130                 135                 140                 
Asp Glu His Glu Ser Ala Leu Arg Ala Leu Gly Pro Gly Ala Thr Phe 
145                 150                 155                 160 
Val Gly Glu Asp His Gly Leu Ala Asn Leu Tyr Asp Ala Ala Met Leu 
                165                 170                 175     
Ser Val Met Trp Gly Leu Leu Asn Gly Phe Leu His Gly Thr Ala Leu 
            180                 185                 190         
Leu Gly Thr Ala Gly Val Ser Ala Thr Ser Val Val Pro Met Leu Gly 
        195                 200                 205             
Gln Gly Ile Gly Val Val Thr Gly Trp Ile Ala Arg Tyr Ala Asp Gln 
    210                 215                 220                 
Ile Asp Ala Gly Val Tyr Pro Ala Asp Asp Ala Thr Ile Asp Thr Gln 
225                 230                 235                 240 
Arg Ala Ala Met Gln His Leu Val Glu Glu Ser Arg Ala Ala Gly Ile 
                245                 250                 255     
Asn Gly Glu Leu Pro Ala Phe Val Thr Lys Leu Ala Asp Arg Ala Val 
            260                 265                 270         
Ala Glu Gly Arg Gly Gly Asp Ser Tyr Ala Ala Leu Ile Glu Gln Phe 
        275                 280                 285             
Arg Lys Ser Ala                                                 
    290                                                         

 
<210> 95
<211> 879
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Imine reductase variant from Pseudonocardia spinosispora
 
<400> 95
atgagtgatt cacctgtgcc cgtgacagtt ctgggtcttg gtctgatggg tcaggcctta 60
gcgggagcgt ttctggcagc cggtcaccct acgacggttt ggaatcgcag cgcagctaaa 120
gctgatgaac tggttggtcg cggtgcaacc ctggccgcgt ctcccgcggc tgccgtcgca 180
gcgggtagtc tgatcgttgt ttgcgtcaca gatcatcgtg cggtgcgcga actgctggaa 240
ccacttggcg atgcactgcc aggtcgcgtt ctggtcaacc tgacgagcgg tacgtcgcgt 300
gaagcccgtg agaacgcaga acatactgcg gctcagggag ccacctacct ggatggtgtg 360
attctgacga ttccaagcgg cattggcacc ccagaagcta ccattctgta ttcgggaccg 420
cgcagtacct tcgatgaaca tgaaagcgca ctgcgcgcat taggtccggg ggcgaccttt 480
gtaggggaag atcacggact ggctgccctg tacgatgccg cacacctgag cgtcatgtgg 540
ggcctgctga atggtttcct gcatggaacc gcactgctgg gaaccgcggg cgtttcggca 600
acctctgtgg ttccgatgct gggtcaaggt atcgaagcgg tgaccggttg gattgcgcgc 660
tacgcagatc agattgatgc cggtgtctat ccagcagacg acaagacgat tgatacccag 720
cgtgctgcaa tgcagcacct ggtcgaggag agccgtgcgg cgggcatcaa tggcgaactt 780
ccggcgtttg tgaccaaact ggccgatcgc gcagtggcag aaggtcgtgg tggtgactct 840
tacgctgcac tgattgaaca gtttcgcaag tcagcttaa                        879

 
<210> 96
<211> 292
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Imine reductase variant from Pseudonocardia spinosispora
 
<400> 96
Met Ser Asp Ser Pro Val Pro Val Thr Val Leu Gly Leu Gly Leu Met 
1               5                   10                  15      
Gly Gln Ala Leu Ala Gly Ala Phe Leu Ala Ala Gly His Pro Thr Thr 
            20                  25                  30          
Val Trp Asn Arg Ser Ala Ala Lys Ala Asp Glu Leu Val Gly Arg Gly 
        35                  40                  45              
Ala Thr Leu Ala Ala Ser Pro Ala Ala Ala Val Ala Ala Gly Ser Leu 
    50                  55                  60                  
Ile Val Val Cys Val Thr Asp His Arg Ala Val Arg Glu Leu Leu Glu 
65                  70                  75                  80  
Pro Leu Gly Asp Ala Leu Pro Gly Arg Val Leu Val Asn Leu Thr Ser 
                85                  90                  95      
Gly Thr Ser Arg Glu Ala Arg Glu Asn Ala Glu His Thr Ala Ala Gln 
            100                 105                 110         
Gly Ala Thr Tyr Leu Asp Gly Val Ile Leu Thr Ile Pro Ser Gly Ile 
        115                 120                 125             
Gly Thr Pro Glu Ala Thr Ile Leu Tyr Ser Gly Pro Arg Ser Thr Phe 
    130                 135                 140                 
Asp Glu His Glu Ser Ala Leu Arg Ala Leu Gly Pro Gly Ala Thr Phe 
145                 150                 155                 160 
Val Gly Glu Asp His Gly Leu Ala Ala Leu Tyr Asp Ala Ala His Leu 
                165                 170                 175     
Ser Val Met Trp Gly Leu Leu Asn Gly Phe Leu His Gly Thr Ala Leu 
            180                 185                 190         
Leu Gly Thr Ala Gly Val Ser Ala Thr Ser Val Val Pro Met Leu Gly 
        195                 200                 205             
Gln Gly Ile Glu Ala Val Thr Gly Trp Ile Ala Arg Tyr Ala Asp Gln 
    210                 215                 220                 
Ile Asp Ala Gly Val Tyr Pro Ala Asp Asp Lys Thr Ile Asp Thr Gln 
225                 230                 235                 240 
Arg Ala Ala Met Gln His Leu Val Glu Glu Ser Arg Ala Ala Gly Ile 
                245                 250                 255     
Asn Gly Glu Leu Pro Ala Phe Val Thr Lys Leu Ala Asp Arg Ala Val 
            260                 265                 270         
Ala Glu Gly Arg Gly Gly Asp Ser Tyr Ala Ala Leu Ile Glu Gln Phe 
        275                 280                 285             
Arg Lys Ser Ala                                                 
    290                                                         

 
<210> 97
<211> 879
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Imine reductase variant from Pseudonocardia spinosispora
 
<400> 97
atgagtgatt cacctgtgcc cgtgacagtt ctgggtcttg gtctgatggg tcaggcctta 60
gcgggagcgt ttctggcagc cggtcaccct acgacggttt ggaatcgcag cgcagctaaa 120
gctgatgaac tggttggtcg cggtgcaacc ctggccgcgt ctcccgcggc tgccgtcgca 180
gcgggtagtc tgatcgttgt ttgcgtcaca gatcatcgtg cggtgcgcga actgctggaa 240
ccacttggcg atgcactgcc aggtcgcgtt ctggtcaacc tgacgagcgg tacgtcgcgt 300
gaagcccgtg agaacgcaga acatactgcg gctcagggag ccacctacct ggatggtgtg 360
attctgacgg gcccaagcgg cattggcacc ccagaagcta ccattctgta ttcgggaccg 420
cgcagtacct tcgatgaaca tgaaagcgca ctgcgcgcat taggtccggg ggcgaccttt 480
gtaggggaag atcacggact ggctaatctg tacgatgccg caatgctgag cgtcatgtgg 540
ggcctgctga atggtttcct gcatggaacc gcactgctgg gaaccgcggg cgtttcggca 600
acctctgtgg ttccgatgct gggtcaaggt atcgaagcgg tgaccggttg gattgcgcgc 660
tacgcagatc agattgatgc cggtgtctat ccagcagacg acgcgacgat tgatacccag 720
cgtgctgcaa tgcagcacct ggtcgaggag agccgtgcgg cgggcatcaa tggcgaactt 780
ccggcgtttg tgaccaaact ggccgatcgc gcagtggcag aaggtcgtgg tggtgactct 840
tacgctgcac tgattgaaca gtttcgcaag tcagcttaa                        879

 
<210> 98
<211> 292
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Imine reductase variant from Pseudonocardia spinosispora
 
<400> 98
Met Ser Asp Ser Pro Val Pro Val Thr Val Leu Gly Leu Gly Leu Met 
1               5                   10                  15      
Gly Gln Ala Leu Ala Gly Ala Phe Leu Ala Ala Gly His Pro Thr Thr 
            20                  25                  30          
Val Trp Asn Arg Ser Ala Ala Lys Ala Asp Glu Leu Val Gly Arg Gly 
        35                  40                  45              
Ala Thr Leu Ala Ala Ser Pro Ala Ala Ala Val Ala Ala Gly Ser Leu 
    50                  55                  60                  
Ile Val Val Cys Val Thr Asp His Arg Ala Val Arg Glu Leu Leu Glu 
65                  70                  75                  80  
Pro Leu Gly Asp Ala Leu Pro Gly Arg Val Leu Val Asn Leu Thr Ser 
                85                  90                  95      
Gly Thr Ser Arg Glu Ala Arg Glu Asn Ala Glu His Thr Ala Ala Gln 
            100                 105                 110         
Gly Ala Thr Tyr Leu Asp Gly Val Ile Leu Thr Gly Pro Ser Gly Ile 
        115                 120                 125             
Gly Thr Pro Glu Ala Thr Ile Leu Tyr Ser Gly Pro Arg Ser Thr Phe 
    130                 135                 140                 
Asp Glu His Glu Ser Ala Leu Arg Ala Leu Gly Pro Gly Ala Thr Phe 
145                 150                 155                 160 
Val Gly Glu Asp His Gly Leu Ala Asn Leu Tyr Asp Ala Ala Met Leu 
                165                 170                 175     
Ser Val Met Trp Gly Leu Leu Asn Gly Phe Leu His Gly Thr Ala Leu 
            180                 185                 190         
Leu Gly Thr Ala Gly Val Ser Ala Thr Ser Val Val Pro Met Leu Gly 
        195                 200                 205             
Gln Gly Ile Glu Ala Val Thr Gly Trp Ile Ala Arg Tyr Ala Asp Gln 
    210                 215                 220                 
Ile Asp Ala Gly Val Tyr Pro Ala Asp Asp Ala Thr Ile Asp Thr Gln 
225                 230                 235                 240 
Arg Ala Ala Met Gln His Leu Val Glu Glu Ser Arg Ala Ala Gly Ile 
                245                 250                 255     
Asn Gly Glu Leu Pro Ala Phe Val Thr Lys Leu Ala Asp Arg Ala Val 
            260                 265                 270         
Ala Glu Gly Arg Gly Gly Asp Ser Tyr Ala Ala Leu Ile Glu Gln Phe 
        275                 280                 285             
Arg Lys Ser Ala                                                 
    290                                                         

 
<210> 99
<211> 879
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Imine reductase variant from Pseudonocardia spinosispora
 
<400> 99
atgagtgatt cacctgtgcc cgtgacagtt ctgggtcttg gtctgatggg tcaggcctta 60
gcgggagcgt ttctggcagc cggtcaccct acgacggttt ggaatcgcag cgcagctaaa 120
gctgatgaac tggttggtcg cggtgcaacc ctggccgcgt ctcccgcggc tgccgtcgca 180
gcgggtagtc tgatcgttgt ttgcgtcaca gatcatcgtg cggtgcgcga actgctggaa 240
ccacttggcg atgcactgcc aggtcgcgtt ctggtcaacc tgacgagcgg tacgtcgcgt 300
gaagcccgtg agaacgcaga acatactgcg gctcagggag ccacctacct ggatggtgtg 360
attctgacga ttccaagcgg cattggcacc ccagaagcta ccattctgta ttcgggaccg 420
cgcagtacct tcgatgaaca tgaaagcgca ctgcgcgcat taggtccggg ggcgaccttt 480
gtaggggaag atcacggact ggctaatctg tacgatgccg caatgctgag cgtcatgtgg 540
ggcctgctga atggtttcct gcatggaacc gcactgctgg gaaccgcggg cgtttcggca 600
acctctgtgg ttccgatgct gggtcaaggt atctgcgctg tgaccggttg gattgcgcgc 660
tacgcagatc agattgatgc cggtgtctat ccagcagacg acaagacgat tgatacccag 720
cgtgctgcaa tgcagcacct ggtcgaggag agccgtgcgg cgggcatcaa tggcgaactt 780
ccggcgtttg tgaccaaact ggccgatcgc gcagtggcag aaggtcgtgg tggtgactct 840
tacgctgcac tgattgaaca gtttcgcaag tcagcttaa                        879

 
<210> 100
<211> 292
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Imine reductase variant from Pseudonocardia spinosispora
 
<400> 100
Met Ser Asp Ser Pro Val Pro Val Thr Val Leu Gly Leu Gly Leu Met 
1               5                   10                  15      
Gly Gln Ala Leu Ala Gly Ala Phe Leu Ala Ala Gly His Pro Thr Thr 
            20                  25                  30          
Val Trp Asn Arg Ser Ala Ala Lys Ala Asp Glu Leu Val Gly Arg Gly 
        35                  40                  45              
Ala Thr Leu Ala Ala Ser Pro Ala Ala Ala Val Ala Ala Gly Ser Leu 
    50                  55                  60                  
Ile Val Val Cys Val Thr Asp His Arg Ala Val Arg Glu Leu Leu Glu 
65                  70                  75                  80  
Pro Leu Gly Asp Ala Leu Pro Gly Arg Val Leu Val Asn Leu Thr Ser 
                85                  90                  95      
Gly Thr Ser Arg Glu Ala Arg Glu Asn Ala Glu His Thr Ala Ala Gln 
            100                 105                 110         
Gly Ala Thr Tyr Leu Asp Gly Val Ile Leu Thr Ile Pro Ser Gly Ile 
        115                 120                 125             
Gly Thr Pro Glu Ala Thr Ile Leu Tyr Ser Gly Pro Arg Ser Thr Phe 
    130                 135                 140                 
Asp Glu His Glu Ser Ala Leu Arg Ala Leu Gly Pro Gly Ala Thr Phe 
145                 150                 155                 160 
Val Gly Glu Asp His Gly Leu Ala Asn Leu Tyr Asp Ala Ala Met Leu 
                165                 170                 175     
Ser Val Met Trp Gly Leu Leu Asn Gly Phe Leu His Gly Thr Ala Leu 
            180                 185                 190         
Leu Gly Thr Ala Gly Val Ser Ala Thr Ser Val Val Pro Met Leu Gly 
        195                 200                 205             
Gln Gly Ile Cys Ala Val Thr Gly Trp Ile Ala Arg Tyr Ala Asp Gln 
    210                 215                 220                 
Ile Asp Ala Gly Val Tyr Pro Ala Asp Asp Lys Thr Ile Asp Thr Gln 
225                 230                 235                 240 
Arg Ala Ala Met Gln His Leu Val Glu Glu Ser Arg Ala Ala Gly Ile 
                245                 250                 255     
Asn Gly Glu Leu Pro Ala Phe Val Thr Lys Leu Ala Asp Arg Ala Val 
            260                 265                 270         
Ala Glu Gly Arg Gly Gly Asp Ser Tyr Ala Ala Leu Ile Glu Gln Phe 
        275                 280                 285             
Arg Lys Ser Ala                                                 
    290                                                         

 
<210> 101
<211> 879
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Imine reductase variant from Pseudonocardia spinosispora
 
<400> 101
atgagtgatt cacctgtgcc cgtgacagtt ctgggtcttg gtctgatggg tcaggcctta 60
gcgggagcgt ttctggcagc cggtcaccct acgacggttt ggaatcgcag cgcagctaaa 120
gctgatgaac tggttggtcg cggtgcaacc ctggccgcgt ctcccgcggc tgccgtcgca 180
gcgggtagtc tgatcgttgt ttgcgtcaca gatcatcgtg cggtgcgcga actgctggaa 240
ccacttggcg atgcactgcc aggtcgcgtt ctggtcaacc tgacgagcgg tacgtcgcgt 300
gaagcccgtg agaacgcaga acatactgcg gctcagggag ccacctacct ggatggtgtg 360
attctgggta ttccaagcgg cattggcacc ccagaagcta ccattctgta ttcgggaccg 420
cgcagtacct tcgatgaaca tgaaagcgca ctgcgcgcat taggtccggg ggcgaccttt 480
gtaggggaag atcacggact ggctgcactg tatgatgcag caatgctgag cgtcatgtgg 540
ggcctgctga atggtttcct gcatggaacc gcactgctgg gaaccgcggg cgtttcggca 600
acctctgtgg ttccgatgct gggtcaaggt atcggagctg tgaccggttg gattgcgcgc 660
tacgcagatc agattgatgc cggtgtctat ccagcagacg acaagacgat tgatacccag 720
cgtgctgcaa tgcagcacct ggtcgaggag agccgtgcgg cgggcatcaa tggcgaactt 780
ccggcgtttg tgaccaaact ggccgatcgc gcagtggcag aaggtcgtgg tggtgactct 840
tacgctgcac tgattgaaca gtttcgcaag tcagcttaa                        879

 
<210> 102
<211> 292
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Imine reductase variant from Pseudonocardia spinosispora
 
<400> 102
Met Ser Asp Ser Pro Val Pro Val Thr Val Leu Gly Leu Gly Leu Met 
1               5                   10                  15      
Gly Gln Ala Leu Ala Gly Ala Phe Leu Ala Ala Gly His Pro Thr Thr 
            20                  25                  30          
Val Trp Asn Arg Ser Ala Ala Lys Ala Asp Glu Leu Val Gly Arg Gly 
        35                  40                  45              
Ala Thr Leu Ala Ala Ser Pro Ala Ala Ala Val Ala Ala Gly Ser Leu 
    50                  55                  60                  
Ile Val Val Cys Val Thr Asp His Arg Ala Val Arg Glu Leu Leu Glu 
65                  70                  75                  80  
Pro Leu Gly Asp Ala Leu Pro Gly Arg Val Leu Val Asn Leu Thr Ser 
                85                  90                  95      
Gly Thr Ser Arg Glu Ala Arg Glu Asn Ala Glu His Thr Ala Ala Gln 
            100                 105                 110         
Gly Ala Thr Tyr Leu Asp Gly Val Ile Leu Gly Ile Pro Ser Gly Ile 
        115                 120                 125             
Gly Thr Pro Glu Ala Thr Ile Leu Tyr Ser Gly Pro Arg Ser Thr Phe 
    130                 135                 140                 
Asp Glu His Glu Ser Ala Leu Arg Ala Leu Gly Pro Gly Ala Thr Phe 
145                 150                 155                 160 
Val Gly Glu Asp His Gly Leu Ala Ala Leu Tyr Asp Ala Ala Met Leu 
                165                 170                 175     
Ser Val Met Trp Gly Leu Leu Asn Gly Phe Leu His Gly Thr Ala Leu 
            180                 185                 190         
Leu Gly Thr Ala Gly Val Ser Ala Thr Ser Val Val Pro Met Leu Gly 
        195                 200                 205             
Gln Gly Ile Gly Ala Val Thr Gly Trp Ile Ala Arg Tyr Ala Asp Gln 
    210                 215                 220                 
Ile Asp Ala Gly Val Tyr Pro Ala Asp Asp Lys Thr Ile Asp Thr Gln 
225                 230                 235                 240 
Arg Ala Ala Met Gln His Leu Val Glu Glu Ser Arg Ala Ala Gly Ile 
                245                 250                 255     
Asn Gly Glu Leu Pro Ala Phe Val Thr Lys Leu Ala Asp Arg Ala Val 
            260                 265                 270         
Ala Glu Gly Arg Gly Gly Asp Ser Tyr Ala Ala Leu Ile Glu Gln Phe 
        275                 280                 285             
Arg Lys Ser Ala                                                 
    290                                                         

 
<210> 103
<211> 879
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Imine reductase variant from Pseudonocardia spinosispora
 
<400> 103
atgagtgatt cacctgtgcc cgtgacagtt ctgggtcttg gtctgatggg tcaggcctta 60
gcgggagcgt ttctggcagc cggtcaccct acgacggttt ggaatcgcag cgcagctaaa 120
gctgatgaac tggttggtcg cggtgcaacc ctggccgcgt ctcccgcggc tgccgtcgca 180
gcgggtagtc tgatcgttgt ttgcgtcaca gatcatcgtg cggtgcgcga actgctggaa 240
ccacttggcg atgcactgcc aggtcgcgtt ctggtcaacc tgacgagcgg tacgtcgcgt 300
gaagcccgtg agaacgcaga acatactgcg gctcagggag ccacctacct ggatggtgtg 360
attctgacga ttccaagcgg cattggcacc ccagaagcta ccattctgta ttcgggaccg 420
cgcagtacct tcgatgaaca tgaaagcgca ctgcgcgcat taggtccggg ggcgaccttt 480
gtaggggaag atcacggact ggctgccctg tacgatattg cacacctgag cgtcatgtgg 540
ggcctgctga atggtttcct gcatggaacc gcactgctgg gaaccgcggg cgtttcggca 600
acctctgtgg ttccgatgct gggtcaaggt atcgaagtgg tgaccggttg gattgcgcgc 660
tacgcagatc agattgatgc cggtgtctat ccagcagacg acgcgacgat tgatacccag 720
cgtgctgcaa tgcagcacct ggtcgaggag agccgtgcgg cgggcatcaa tggcgaactt 780
ccggcgtttg tgaccaaact ggccgatcgc gcagtggcag aaggtcgtgg tggtgactct 840
tacgctgcac tgattgaaca gtttcgcaag tcagcttaa                        879

 
<210> 104
<211> 292
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Imine reductase variant from Pseudonocardia spinosispora
 
<400> 104
Met Ser Asp Ser Pro Val Pro Val Thr Val Leu Gly Leu Gly Leu Met 
1               5                   10                  15      
Gly Gln Ala Leu Ala Gly Ala Phe Leu Ala Ala Gly His Pro Thr Thr 
            20                  25                  30          
Val Trp Asn Arg Ser Ala Ala Lys Ala Asp Glu Leu Val Gly Arg Gly 
        35                  40                  45              
Ala Thr Leu Ala Ala Ser Pro Ala Ala Ala Val Ala Ala Gly Ser Leu 
    50                  55                  60                  
Ile Val Val Cys Val Thr Asp His Arg Ala Val Arg Glu Leu Leu Glu 
65                  70                  75                  80  
Pro Leu Gly Asp Ala Leu Pro Gly Arg Val Leu Val Asn Leu Thr Ser 
                85                  90                  95      
Gly Thr Ser Arg Glu Ala Arg Glu Asn Ala Glu His Thr Ala Ala Gln 
            100                 105                 110         
Gly Ala Thr Tyr Leu Asp Gly Val Ile Leu Thr Ile Pro Ser Gly Ile 
        115                 120                 125             
Gly Thr Pro Glu Ala Thr Ile Leu Tyr Ser Gly Pro Arg Ser Thr Phe 
    130                 135                 140                 
Asp Glu His Glu Ser Ala Leu Arg Ala Leu Gly Pro Gly Ala Thr Phe 
145                 150                 155                 160 
Val Gly Glu Asp His Gly Leu Ala Ala Leu Tyr Asp Ile Ala His Leu 
                165                 170                 175     
Ser Val Met Trp Gly Leu Leu Asn Gly Phe Leu His Gly Thr Ala Leu 
            180                 185                 190         
Leu Gly Thr Ala Gly Val Ser Ala Thr Ser Val Val Pro Met Leu Gly 
        195                 200                 205             
Gln Gly Ile Glu Val Val Thr Gly Trp Ile Ala Arg Tyr Ala Asp Gln 
    210                 215                 220                 
Ile Asp Ala Gly Val Tyr Pro Ala Asp Asp Ala Thr Ile Asp Thr Gln 
225                 230                 235                 240 
Arg Ala Ala Met Gln His Leu Val Glu Glu Ser Arg Ala Ala Gly Ile 
                245                 250                 255     
Asn Gly Glu Leu Pro Ala Phe Val Thr Lys Leu Ala Asp Arg Ala Val 
            260                 265                 270         
Ala Glu Gly Arg Gly Gly Asp Ser Tyr Ala Ala Leu Ile Glu Gln Phe 
        275                 280                 285             
Arg Lys Ser Ala                                                 
    290                                                         

 
<210> 105
<211> 879
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Imine reductase variant from Pseudonocardia spinosispora
 
<400> 105
atgagtgatt cacctgtgcc cgtgacagtt ctgggtcttg gtctgatggg tcaggcctta 60
gcgggagcgt ttctggcagc cggtcaccct acgacggttt ggaatcgcag cgcagctaaa 120
gctgatgaac tggttggtcg cggtgcaacc ctggccgcgt ctcccgcggc tgccgtcgca 180
gcgggtagtc tgatcgttgt ttgcgtcaca gatcatcgtg cggtgcgcga actgctggaa 240
ccacttggcg atgcactgcc aggtcgcgtt ctggtcaacc tgacgagcgg tacgtcgcgt 300
gaagcccgtg agaacgcaga acatactgcg gctcagggag ccacctacct ggatggtgtg 360
attctgacga ttccaagcgg cattggcacc ccagaagcta ccattctgta ttcgggaccg 420
cgcagtacct tcgatgaaca tgaaagcgca ctgcgcgcat taggtccggg ggcgaccttt 480
gtaggggaag atcacggact ggctgcactg tatgatgcag caatgctgag cgtcatgtgg 540
ggcctgctga atggtttcct gcatggaacc gcactgctgg gaaccgcggg cgtttcggca 600
acctctgtgg ttccgatgct gggtcaaggt atcgaaactg tgaccggttg gattgcgcgc 660
tacgcagatc agattgatgc cggtgtctat ccagcagacg acaagacgat tgatacccag 720
cgtgctgcaa tgcagcacct ggtcgaggag agccgtgcgg cgggcatcaa tggcgaactt 780
ccggcgtttg tgaccaaact ggccgatcgc gcagtggcag aaggtcgtgg tggtgactct 840
tacgctgcac tgattgaaca gtttcgcaag tcagcttaa                        879

 
<210> 106
<211> 292
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Imine reductase variant from Pseudonocardia spinosispora
 
<400> 106
Met Ser Asp Ser Pro Val Pro Val Thr Val Leu Gly Leu Gly Leu Met 
1               5                   10                  15      
Gly Gln Ala Leu Ala Gly Ala Phe Leu Ala Ala Gly His Pro Thr Thr 
            20                  25                  30          
Val Trp Asn Arg Ser Ala Ala Lys Ala Asp Glu Leu Val Gly Arg Gly 
        35                  40                  45              
Ala Thr Leu Ala Ala Ser Pro Ala Ala Ala Val Ala Ala Gly Ser Leu 
    50                  55                  60                  
Ile Val Val Cys Val Thr Asp His Arg Ala Val Arg Glu Leu Leu Glu 
65                  70                  75                  80  
Pro Leu Gly Asp Ala Leu Pro Gly Arg Val Leu Val Asn Leu Thr Ser 
                85                  90                  95      
Gly Thr Ser Arg Glu Ala Arg Glu Asn Ala Glu His Thr Ala Ala Gln 
            100                 105                 110         
Gly Ala Thr Tyr Leu Asp Gly Val Ile Leu Thr Ile Pro Ser Gly Ile 
        115                 120                 125             
Gly Thr Pro Glu Ala Thr Ile Leu Tyr Ser Gly Pro Arg Ser Thr Phe 
    130                 135                 140                 
Asp Glu His Glu Ser Ala Leu Arg Ala Leu Gly Pro Gly Ala Thr Phe 
145                 150                 155                 160 
Val Gly Glu Asp His Gly Leu Ala Ala Leu Tyr Asp Ala Ala Met Leu 
                165                 170                 175     
Ser Val Met Trp Gly Leu Leu Asn Gly Phe Leu His Gly Thr Ala Leu 
            180                 185                 190         
Leu Gly Thr Ala Gly Val Ser Ala Thr Ser Val Val Pro Met Leu Gly 
        195                 200                 205             
Gln Gly Ile Glu Thr Val Thr Gly Trp Ile Ala Arg Tyr Ala Asp Gln 
    210                 215                 220                 
Ile Asp Ala Gly Val Tyr Pro Ala Asp Asp Lys Thr Ile Asp Thr Gln 
225                 230                 235                 240 
Arg Ala Ala Met Gln His Leu Val Glu Glu Ser Arg Ala Ala Gly Ile 
                245                 250                 255     
Asn Gly Glu Leu Pro Ala Phe Val Thr Lys Leu Ala Asp Arg Ala Val 
            260                 265                 270         
Ala Glu Gly Arg Gly Gly Asp Ser Tyr Ala Ala Leu Ile Glu Gln Phe 
        275                 280                 285             
Arg Lys Ser Ala                                                 
    290                                                         

 
<210> 107
<211> 879
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Imine reductase variant from Pseudonocardia spinosispora
 
<400> 107
atgagtgatt cacctgtgcc cgtgacagtt ctgggtcttg gtctgatggg tcaggcctta 60
gcgggagcgt ttctggcagc cggtcaccct acgacggttt ggaatcgcag cgcagctaaa 120
gctgatgaac tggttggtcg cggtgcaacc ctggccgcgt ctcccgcggc tgccgtcgca 180
gcgggtagtc tgatcgttgt ttgcgtcaca gatcatcgtg cggtgcgcga actgctggaa 240
ccacttggcg atgcactgcc aggtcgcgtt ctggtcaacc tgacgagcgg tacgtcgcgt 300
gaagcccgtg agaacgcaga acatactgcg gctcagggag ccacctacct ggatggtgtg 360
attctgacca ttccaagcgg cattggcacc ccagaagcta ccattctgta ttcgggaccg 420
cgcagtacct tcgatgaaca tgaaagcgca ctgcgcgcat taggtccggg ggcgaccttt 480
gtaggggaag atcacggact ggctgccctg tacgatgccg cacacctgag cgtcatgtgg 540
ggcctgctga atggtttcct gcatggaacc gcactgctgg gaaccgcggg cgtttcggca 600
acctctgtgg ttccgatgct gggtcaaggt atcgaacctg tgaccggttg gattgcgcgc 660
tacgcagatc agattgatgc cggtgtctat ccagcagacg acaagacgat tgatacccag 720
cgtgctgcaa tgcagcacct ggtcgaggag agccgtgcgg cgggcatcaa tggcgaactt 780
ccggcgtttg tgaccaaact ggccgatcgc gcagtggcag aaggtcgtgg tggtgactct 840
tacgctgcac tgattgaaca gtttcgcaag tcagcttaa                        879

 
<210> 108
<211> 292
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Imine reductase variant from Pseudonocardia spinosispora
 
<400> 108
Met Ser Asp Ser Pro Val Pro Val Thr Val Leu Gly Leu Gly Leu Met 
1               5                   10                  15      
Gly Gln Ala Leu Ala Gly Ala Phe Leu Ala Ala Gly His Pro Thr Thr 
            20                  25                  30          
Val Trp Asn Arg Ser Ala Ala Lys Ala Asp Glu Leu Val Gly Arg Gly 
        35                  40                  45              
Ala Thr Leu Ala Ala Ser Pro Ala Ala Ala Val Ala Ala Gly Ser Leu 
    50                  55                  60                  
Ile Val Val Cys Val Thr Asp His Arg Ala Val Arg Glu Leu Leu Glu 
65                  70                  75                  80  
Pro Leu Gly Asp Ala Leu Pro Gly Arg Val Leu Val Asn Leu Thr Ser 
                85                  90                  95      
Gly Thr Ser Arg Glu Ala Arg Glu Asn Ala Glu His Thr Ala Ala Gln 
            100                 105                 110         
Gly Ala Thr Tyr Leu Asp Gly Val Ile Leu Thr Ile Pro Ser Gly Ile 
        115                 120                 125             
Gly Thr Pro Glu Ala Thr Ile Leu Tyr Ser Gly Pro Arg Ser Thr Phe 
    130                 135                 140                 
Asp Glu His Glu Ser Ala Leu Arg Ala Leu Gly Pro Gly Ala Thr Phe 
145                 150                 155                 160 
Val Gly Glu Asp His Gly Leu Ala Ala Leu Tyr Asp Ala Ala His Leu 
                165                 170                 175     
Ser Val Met Trp Gly Leu Leu Asn Gly Phe Leu His Gly Thr Ala Leu 
            180                 185                 190         
Leu Gly Thr Ala Gly Val Ser Ala Thr Ser Val Val Pro Met Leu Gly 
        195                 200                 205             
Gln Gly Ile Glu Pro Val Thr Gly Trp Ile Ala Arg Tyr Ala Asp Gln 
    210                 215                 220                 
Ile Asp Ala Gly Val Tyr Pro Ala Asp Asp Lys Thr Ile Asp Thr Gln 
225                 230                 235                 240 
Arg Ala Ala Met Gln His Leu Val Glu Glu Ser Arg Ala Ala Gly Ile 
                245                 250                 255     
Asn Gly Glu Leu Pro Ala Phe Val Thr Lys Leu Ala Asp Arg Ala Val 
            260                 265                 270         
Ala Glu Gly Arg Gly Gly Asp Ser Tyr Ala Ala Leu Ile Glu Gln Phe 
        275                 280                 285             
Arg Lys Ser Ala                                                 
    290                                                         

 
<210> 109
<211> 879
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Imine reductase variant from Pseudonocardia spinosispora
 
<400> 109
atgagtgatt cacctgtgcc cgtgacagtt ctgggtcttg gtctgatggg tcaggcctta 60
gcgggagcgt ttctggcagc cggtcaccct acgacggttt ggaatcgcag cgcagctaaa 120
gctgatgaac tggttggtcg cggtgcaacc ctggccgcgt ctcccgcggc tgccgtcgca 180
gcgggtagtc tgatcgttgt ttgcgtcaca gatcatcgtg cggtgcgcga actgctggaa 240
ccacttggcg atgcactgcc aggtcgcgtt ctggtcaacc tgacgagcgg tacgtcgcgt 300
gaagcccgtg agaacgcaga acatactgcg gctcagggag ccacctacct ggatggtgtg 360
attctgacgg gcccaagcgg cattggcacc ccagaagcta ccattctgta ttcgggaccg 420
cgcagtacct tcgatgaaca tgaaagcgca ctgcgcgcat taggtccggg ggcgaccttt 480
gtaggggaag atcacggact ggctaatctg tacgatgccg caatgctgag cgtcatgtgg 540
ggcctgctga atggtttcct gcatggaacc gcactgctgg gaaccgcggg cgtttcggca 600
acctctgtgg ttccgatgct gggtcaaggt atcggagtgg tgaccggttg gattgcgcgc 660
tacgcagatc agattgatgc cggtgtctat ccagcagacg acaagacgat tgatacccag 720
cgtgctgcaa tgcagcacct ggtcgaggag agccgtgcgg cgggcatcaa tggcgaactt 780
ccggcgtttg tgaccaaact ggccgatcgc gcagtggcag aaggtcgtgg tggtgactct 840
tacgctgcac tgattgaaca gtttcgcaag tcagcttaa                        879

 
<210> 110
<211> 292
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Imine reductase variant from Pseudonocardia spinosispora
 
<400> 110
Met Ser Asp Ser Pro Val Pro Val Thr Val Leu Gly Leu Gly Leu Met 
1               5                   10                  15      
Gly Gln Ala Leu Ala Gly Ala Phe Leu Ala Ala Gly His Pro Thr Thr 
            20                  25                  30          
Val Trp Asn Arg Ser Ala Ala Lys Ala Asp Glu Leu Val Gly Arg Gly 
        35                  40                  45              
Ala Thr Leu Ala Ala Ser Pro Ala Ala Ala Val Ala Ala Gly Ser Leu 
    50                  55                  60                  
Ile Val Val Cys Val Thr Asp His Arg Ala Val Arg Glu Leu Leu Glu 
65                  70                  75                  80  
Pro Leu Gly Asp Ala Leu Pro Gly Arg Val Leu Val Asn Leu Thr Ser 
                85                  90                  95      
Gly Thr Ser Arg Glu Ala Arg Glu Asn Ala Glu His Thr Ala Ala Gln 
            100                 105                 110         
Gly Ala Thr Tyr Leu Asp Gly Val Ile Leu Thr Gly Pro Ser Gly Ile 
        115                 120                 125             
Gly Thr Pro Glu Ala Thr Ile Leu Tyr Ser Gly Pro Arg Ser Thr Phe 
    130                 135                 140                 
Asp Glu His Glu Ser Ala Leu Arg Ala Leu Gly Pro Gly Ala Thr Phe 
145                 150                 155                 160 
Val Gly Glu Asp His Gly Leu Ala Asn Leu Tyr Asp Ala Ala Met Leu 
                165                 170                 175     
Ser Val Met Trp Gly Leu Leu Asn Gly Phe Leu His Gly Thr Ala Leu 
            180                 185                 190         
Leu Gly Thr Ala Gly Val Ser Ala Thr Ser Val Val Pro Met Leu Gly 
        195                 200                 205             
Gln Gly Ile Gly Val Val Thr Gly Trp Ile Ala Arg Tyr Ala Asp Gln 
    210                 215                 220                 
Ile Asp Ala Gly Val Tyr Pro Ala Asp Asp Lys Thr Ile Asp Thr Gln 
225                 230                 235                 240 
Arg Ala Ala Met Gln His Leu Val Glu Glu Ser Arg Ala Ala Gly Ile 
                245                 250                 255     
Asn Gly Glu Leu Pro Ala Phe Val Thr Lys Leu Ala Asp Arg Ala Val 
            260                 265                 270         
Ala Glu Gly Arg Gly Gly Asp Ser Tyr Ala Ala Leu Ile Glu Gln Phe 
        275                 280                 285             
Arg Lys Ser Ala                                                 
    290                                                         

 
<210> 111
<211> 879
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Imine reductase variant from Pseudonocardia spinosispora
 
<400> 111
atgagtgatt cacctgtgcc cgtgacagtt ctgggtcttg gtctgatggg tcaggcctta 60
gcgggagcgt ttctggcagc cggtcaccct acgacggttt ggaatcgcag cgcagctaaa 120
gctgatgaac tggttggtcg cggtgcaacc ctggccgcgt ctcccgcggc tgccgtcgca 180
gcgggtagtc tgatcgttgt ttgcgtcaca gatcatcgtg cggtgcgcga actgctggaa 240
ccacttggcg atgcactgcc aggtcgcgtt ctggtcaacc tgacgagcgg tacgtcgcgt 300
gaagcccgtg agaacgcaga acatactgcg gctcagggag ccacctacct ggatggtgtg 360
attctgacgg gcccaagcgg cattggcacc ccagaagcta ccattctgta ttcgggaccg 420
cgcagtacct tcgatgaaca tgaaagcgca ctgcgcgcat taggtccggg ggcgaccttt 480
gtaggggaag atcacggact ggctaatctg tacgatgccg caatgctgag cgtcatgtgg 540
ggcctgctga atggtttcct gcatggaacc gcactgctgg gaaccgcggg cgtttcggca 600
acctctgtgg ttccgatgct gggtcaaggt atcgtagtgg tgaccggttg gattgcgcgc 660
tacgcagatc agattgatgc cggtgtctat ccagcagacg acaagacgat tgatacccag 720
cgtgctgcaa tgcagcacct ggtcgaggag agccgtgcgg cgggcatcaa tggcgaactt 780
ccggcgtttg tgaccaaact ggccgatcgc gcagtggcag aaggtcgtgg tggtgactct 840
tacgctgcac tgattgaaca gtttcgcaag tcagcttaa                        879

 
<210> 112
<211> 292
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Imine reductase variant from Pseudonocardia spinosispora
 
<400> 112
Met Ser Asp Ser Pro Val Pro Val Thr Val Leu Gly Leu Gly Leu Met 
1               5                   10                  15      
Gly Gln Ala Leu Ala Gly Ala Phe Leu Ala Ala Gly His Pro Thr Thr 
            20                  25                  30          
Val Trp Asn Arg Ser Ala Ala Lys Ala Asp Glu Leu Val Gly Arg Gly 
        35                  40                  45              
Ala Thr Leu Ala Ala Ser Pro Ala Ala Ala Val Ala Ala Gly Ser Leu 
    50                  55                  60                  
Ile Val Val Cys Val Thr Asp His Arg Ala Val Arg Glu Leu Leu Glu 
65                  70                  75                  80  
Pro Leu Gly Asp Ala Leu Pro Gly Arg Val Leu Val Asn Leu Thr Ser 
                85                  90                  95      
Gly Thr Ser Arg Glu Ala Arg Glu Asn Ala Glu His Thr Ala Ala Gln 
            100                 105                 110         
Gly Ala Thr Tyr Leu Asp Gly Val Ile Leu Thr Gly Pro Ser Gly Ile 
        115                 120                 125             
Gly Thr Pro Glu Ala Thr Ile Leu Tyr Ser Gly Pro Arg Ser Thr Phe 
    130                 135                 140                 
Asp Glu His Glu Ser Ala Leu Arg Ala Leu Gly Pro Gly Ala Thr Phe 
145                 150                 155                 160 
Val Gly Glu Asp His Gly Leu Ala Asn Leu Tyr Asp Ala Ala Met Leu 
                165                 170                 175     
Ser Val Met Trp Gly Leu Leu Asn Gly Phe Leu His Gly Thr Ala Leu 
            180                 185                 190         
Leu Gly Thr Ala Gly Val Ser Ala Thr Ser Val Val Pro Met Leu Gly 
        195                 200                 205             
Gln Gly Ile Val Val Val Thr Gly Trp Ile Ala Arg Tyr Ala Asp Gln 
    210                 215                 220                 
Ile Asp Ala Gly Val Tyr Pro Ala Asp Asp Lys Thr Ile Asp Thr Gln 
225                 230                 235                 240 
Arg Ala Ala Met Gln His Leu Val Glu Glu Ser Arg Ala Ala Gly Ile 
                245                 250                 255     
Asn Gly Glu Leu Pro Ala Phe Val Thr Lys Leu Ala Asp Arg Ala Val 
            260                 265                 270         
Ala Glu Gly Arg Gly Gly Asp Ser Tyr Ala Ala Leu Ile Glu Gln Phe 
        275                 280                 285             
Arg Lys Ser Ala                                                 
    290                                                         

 
<210> 113
<211> 879
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Imine reductase variant from Pseudonocardia spinosispora
 
<400> 113
atgagtgatt cacctgtgcc cgtgacagtt ctgggtcttg gtctgatggg tcaggcctta 60
gcgggagcgt ttctggcagc cggtcaccct acgacggttt ggaatcgcag cgcagctaaa 120
gctgatgaac tggttggtcg cggtgcaacc ctggccgcgt ctcccgcggc tgccgtcgca 180
gcgggtagtc tgatcgttgt ttgcgtcaca gatcatcgtg cggtgcgcga actgctggaa 240
ccacttggcg atgcactgcc aggtcgcgtt ctggtcaacc tgacgagcgg tacgtcgcgt 300
gaagcccgtg agaacgcaga acatactgcg gctcagggag ccacctacct ggatggtgtg 360
attctgggta ttccaagcgg cattggcacc ccagaagcta ccattctgta ttcgggaccg 420
cgcagtacct tcgatgaaca tgaaagcgca ctgcgcgcat taggtccggg ggcgaccttt 480
gtaggggaag atcacggact ggctaatctg tacgatgccg caatgctgag cgtcatgtgg 540
ggcctgctga atggtttcct gcatggaacc gcactgctgg gaaccgcggg cgtttcggca 600
acctctgtgg ttccgatgct gggtcaaggt atcggagtgg tgaccggttg gattgcgcgc 660
tacgcagatc agattgatgc cggtgtctat ccagcagacg acgcgacgat tgatacccag 720
cgtgctgcaa tgcagcacct ggtcgaggag agccgtgcgg cgggcatcaa tggcgaactt 780
ccggcgtttg tgaccaaact ggccgatcgc gcagtggcag aaggtcgtgg tggtgactct 840
tacgctgcac tgattgaaca gtttcgcaag tcagcttaa                        879

 
<210> 114
<211> 292
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Imine reductase variant from Pseudonocardia spinosispora
 
<400> 114
Met Ser Asp Ser Pro Val Pro Val Thr Val Leu Gly Leu Gly Leu Met 
1               5                   10                  15      
Gly Gln Ala Leu Ala Gly Ala Phe Leu Ala Ala Gly His Pro Thr Thr 
            20                  25                  30          
Val Trp Asn Arg Ser Ala Ala Lys Ala Asp Glu Leu Val Gly Arg Gly 
        35                  40                  45              
Ala Thr Leu Ala Ala Ser Pro Ala Ala Ala Val Ala Ala Gly Ser Leu 
    50                  55                  60                  
Ile Val Val Cys Val Thr Asp His Arg Ala Val Arg Glu Leu Leu Glu 
65                  70                  75                  80  
Pro Leu Gly Asp Ala Leu Pro Gly Arg Val Leu Val Asn Leu Thr Ser 
                85                  90                  95      
Gly Thr Ser Arg Glu Ala Arg Glu Asn Ala Glu His Thr Ala Ala Gln 
            100                 105                 110         
Gly Ala Thr Tyr Leu Asp Gly Val Ile Leu Gly Ile Pro Ser Gly Ile 
        115                 120                 125             
Gly Thr Pro Glu Ala Thr Ile Leu Tyr Ser Gly Pro Arg Ser Thr Phe 
    130                 135                 140                 
Asp Glu His Glu Ser Ala Leu Arg Ala Leu Gly Pro Gly Ala Thr Phe 
145                 150                 155                 160 
Val Gly Glu Asp His Gly Leu Ala Asn Leu Tyr Asp Ala Ala Met Leu 
                165                 170                 175     
Ser Val Met Trp Gly Leu Leu Asn Gly Phe Leu His Gly Thr Ala Leu 
            180                 185                 190         
Leu Gly Thr Ala Gly Val Ser Ala Thr Ser Val Val Pro Met Leu Gly 
        195                 200                 205             
Gln Gly Ile Gly Val Val Thr Gly Trp Ile Ala Arg Tyr Ala Asp Gln 
    210                 215                 220                 
Ile Asp Ala Gly Val Tyr Pro Ala Asp Asp Ala Thr Ile Asp Thr Gln 
225                 230                 235                 240 
Arg Ala Ala Met Gln His Leu Val Glu Glu Ser Arg Ala Ala Gly Ile 
                245                 250                 255     
Asn Gly Glu Leu Pro Ala Phe Val Thr Lys Leu Ala Asp Arg Ala Val 
            260                 265                 270         
Ala Glu Gly Arg Gly Gly Asp Ser Tyr Ala Ala Leu Ile Glu Gln Phe 
        275                 280                 285             
Arg Lys Ser Ala                                                 
    290                                                         

 
<210> 115
<211> 879
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Imine reductase variant from Pseudonocardia spinosispora
 
<400> 115
atgagtgatt cacctgtgcc cgtgacagtt ctgggtcttg gtctgatggg tcaggcctta 60
gcgggagcgt ttctggcagc cggtcaccct acgacggttt ggaatcgcag cgcagctaaa 120
gctgatgaac tggttggtcg cggtgcaacc ctggccgcgt ctcccgcggc tgccgtcgca 180
gcgggtagtc tgatcgttgt ttgcgtcaca gatcatcgtg cggtgcgcga actgctggaa 240
ccacttggcg atgcactgcc aggtcgcgtt ctggtcaacc tgacgagcgg tacgtcgcgt 300
gaagcccgtg agaacgcaga acatactgcg gctcagggag ccacctacct ggatggtgtg 360
attctgacga ttccaagcgg cattggcacc ccagaagcta ccattctgta ttcgggaccg 420
cgcagtacct tcgatgaaca tgaaagcgca ctgcgcgcat taggtccggg ggcgaccttt 480
gtaggggaag atcacggact ggctgcactg tatgatgcag caatgctgag cgtcatgtgg 540
ggcctgctga atggtttcct gcatggaacc gcactgctgg gaaccgcggg cgtttcggca 600
acctctgtgg ttccgatgct gggtcaaggt atcgtagtgg tgaccggttg gattgcgcgc 660
tacgcagatc agattgatgc cggtgtctat ccagcagacg acaagacgat tgatacccag 720
cgtgctgcaa tgcagcacct ggtcgaggag agccgtgcgg cgggcatcaa tggcgaactt 780
ccggcgtttg tgaccaaact ggccgatcgc gcagtggcag aaggtcgtgg tggtgactct 840
tacgctgcac tgattgaaca gtttcgcaag tcagcttaa                        879

 
<210> 116
<211> 292
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Imine reductase variant from Pseudonocardia spinosispora
 
<400> 116
Met Ser Asp Ser Pro Val Pro Val Thr Val Leu Gly Leu Gly Leu Met 
1               5                   10                  15      
Gly Gln Ala Leu Ala Gly Ala Phe Leu Ala Ala Gly His Pro Thr Thr 
            20                  25                  30          
Val Trp Asn Arg Ser Ala Ala Lys Ala Asp Glu Leu Val Gly Arg Gly 
        35                  40                  45              
Ala Thr Leu Ala Ala Ser Pro Ala Ala Ala Val Ala Ala Gly Ser Leu 
    50                  55                  60                  
Ile Val Val Cys Val Thr Asp His Arg Ala Val Arg Glu Leu Leu Glu 
65                  70                  75                  80  
Pro Leu Gly Asp Ala Leu Pro Gly Arg Val Leu Val Asn Leu Thr Ser 
                85                  90                  95      
Gly Thr Ser Arg Glu Ala Arg Glu Asn Ala Glu His Thr Ala Ala Gln 
            100                 105                 110         
Gly Ala Thr Tyr Leu Asp Gly Val Ile Leu Thr Ile Pro Ser Gly Ile 
        115                 120                 125             
Gly Thr Pro Glu Ala Thr Ile Leu Tyr Ser Gly Pro Arg Ser Thr Phe 
    130                 135                 140                 
Asp Glu His Glu Ser Ala Leu Arg Ala Leu Gly Pro Gly Ala Thr Phe 
145                 150                 155                 160 
Val Gly Glu Asp His Gly Leu Ala Ala Leu Tyr Asp Ala Ala Met Leu 
                165                 170                 175     
Ser Val Met Trp Gly Leu Leu Asn Gly Phe Leu His Gly Thr Ala Leu 
            180                 185                 190         
Leu Gly Thr Ala Gly Val Ser Ala Thr Ser Val Val Pro Met Leu Gly 
        195                 200                 205             
Gln Gly Ile Val Val Val Thr Gly Trp Ile Ala Arg Tyr Ala Asp Gln 
    210                 215                 220                 
Ile Asp Ala Gly Val Tyr Pro Ala Asp Asp Lys Thr Ile Asp Thr Gln 
225                 230                 235                 240 
Arg Ala Ala Met Gln His Leu Val Glu Glu Ser Arg Ala Ala Gly Ile 
                245                 250                 255     
Asn Gly Glu Leu Pro Ala Phe Val Thr Lys Leu Ala Asp Arg Ala Val 
            260                 265                 270         
Ala Glu Gly Arg Gly Gly Asp Ser Tyr Ala Ala Leu Ile Glu Gln Phe 
        275                 280                 285             
Arg Lys Ser Ala                                                 
    290                                                         

 
<210> 117
<211> 879
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Imine reductase variant from Pseudonocardia spinosispora
 
<400> 117
atgagtgatt cacctgtgcc cgtgacagtt ctgggtcttg gtctgatggg tcaggcctta 60
gcgggagcgt ttctggcagc cggtcaccct acgacggttt ggaatcgcag cgcagctaaa 120
gctgatgaac tggttggtcg cggtgcaacc ctggccgcgt ctcccgcggc tgccgtcgca 180
gcgggtagtc tgatcgttgt ttgcgtcaca gatcatcgtg cggtgcgcga actgctggaa 240
ccacttggcg atgcactgcc aggtcgcgtt ctggtcaacc tgacgagcgg tacgtcgcgt 300
gaagcccgtg agaacgcaga acatactgcg gctcagggag ccacctacct ggatggtgtg 360
attctgggta ttccaagcgg cattggcacc ccagaagcta ccattctgta ttcgggaccg 420
cgcagtacct tcgatgaaca tgaaagcgca ctgcgcgcat taggtccggg ggcgaccttt 480
gtaggggaag atcacggact ggctgcactg tatgatgcag caatgctgag cgtcatgtgg 540
ggcctgctga atggtttcct gcatggaacc gcactgctgg gaaccgcggg cgtttcggca 600
acctctgtgg ttccgatgct gggtcaaggt atctgcgtgg tgaccggttg gattgcgcgc 660
tacgcagatc agattgatgc cggtgtctat ccagcagacg acaagacgat tgatacccag 720
cgtgctgcaa tgcagcacct ggtcgaggag agccgtgcgg cgggcatcaa tggcgaactt 780
ccggcgtttg tgaccaaact ggccgatcgc gcagtggcag aaggtcgtgg tggtgactct 840
tacgctgcac tgattgaaca gtttcgcaag tcagcttaa                        879

 
<210> 118
<211> 292
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Imine reductase variant from Pseudonocardia spinosispora
 
<400> 118
Met Ser Asp Ser Pro Val Pro Val Thr Val Leu Gly Leu Gly Leu Met 
1               5                   10                  15      
Gly Gln Ala Leu Ala Gly Ala Phe Leu Ala Ala Gly His Pro Thr Thr 
            20                  25                  30          
Val Trp Asn Arg Ser Ala Ala Lys Ala Asp Glu Leu Val Gly Arg Gly 
        35                  40                  45              
Ala Thr Leu Ala Ala Ser Pro Ala Ala Ala Val Ala Ala Gly Ser Leu 
    50                  55                  60                  
Ile Val Val Cys Val Thr Asp His Arg Ala Val Arg Glu Leu Leu Glu 
65                  70                  75                  80  
Pro Leu Gly Asp Ala Leu Pro Gly Arg Val Leu Val Asn Leu Thr Ser 
                85                  90                  95      
Gly Thr Ser Arg Glu Ala Arg Glu Asn Ala Glu His Thr Ala Ala Gln 
            100                 105                 110         
Gly Ala Thr Tyr Leu Asp Gly Val Ile Leu Gly Ile Pro Ser Gly Ile 
        115                 120                 125             
Gly Thr Pro Glu Ala Thr Ile Leu Tyr Ser Gly Pro Arg Ser Thr Phe 
    130                 135                 140                 
Asp Glu His Glu Ser Ala Leu Arg Ala Leu Gly Pro Gly Ala Thr Phe 
145                 150                 155                 160 
Val Gly Glu Asp His Gly Leu Ala Ala Leu Tyr Asp Ala Ala Met Leu 
                165                 170                 175     
Ser Val Met Trp Gly Leu Leu Asn Gly Phe Leu His Gly Thr Ala Leu 
            180                 185                 190         
Leu Gly Thr Ala Gly Val Ser Ala Thr Ser Val Val Pro Met Leu Gly 
        195                 200                 205             
Gln Gly Ile Cys Val Val Thr Gly Trp Ile Ala Arg Tyr Ala Asp Gln 
    210                 215                 220                 
Ile Asp Ala Gly Val Tyr Pro Ala Asp Asp Lys Thr Ile Asp Thr Gln 
225                 230                 235                 240 
Arg Ala Ala Met Gln His Leu Val Glu Glu Ser Arg Ala Ala Gly Ile 
                245                 250                 255     
Asn Gly Glu Leu Pro Ala Phe Val Thr Lys Leu Ala Asp Arg Ala Val 
            260                 265                 270         
Ala Glu Gly Arg Gly Gly Asp Ser Tyr Ala Ala Leu Ile Glu Gln Phe 
        275                 280                 285             
Arg Lys Ser Ala                                                 
    290                                                         

 
<210> 119
<211> 879
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Imine reductase variant from Pseudonocardia spinosispora
 
<400> 119
atgagtgatt cacctgtgcc cgtgacagtt ctgggtcttg gtctgatggg tcaggcctta 60
gcgggagcgt ttctggcagc cggtcaccct acgacggttt ggaatcgcag cgcagctaaa 120
gctgatgaac tggttggtcg cggtgcaacc ctggccgcgt ctcccgcggc tgccgtcgca 180
gcgggtagtc tgatcgttgt ttgcgtcaca gatcatcgtg cggtgcgcga actgctggaa 240
ccacttggcg atgcactgcc aggtcgcgtt ctggtcaacc tgacgagcgg tacgtcgcgt 300
gaagcccgtg agaacgcaga acatactgcg gctcagggag ccacctacct ggatggtgtg 360
attctgggta ttccaagcgg cattggcacc ccagaagcta ccattctgta ttcgggaccg 420
cgcagtacct tcgatgaaca tgaaagcgca ctgcgcgcat taggtccggg ggcgaccttt 480
gtaggggaag atcacggact ggctaatctg gttgatcatg caatgctgag cgtcatgtgg 540
ggcctgctga atggtttcct gcatggaacc gcactgctgg gaaccgcggg cgtttcggca 600
acctctgtgg ttccgatgct gggtcaaggt atcgaagtgg tgaccggttg gattgcgcgc 660
tacgcagatc agattgatgc cggtgtctat ccagcagacg acaagacgat tgatacccag 720
cgtgctgcaa tgcagcacct ggtcgaggag agccgtgcgg cgggcatcaa tggcgaactt 780
ccggcgtttg tgaccaaact ggccgatcgc gcagtggcag aaggtcgtgg tggtgactct 840
tacgctgcac tgattgaaca gtttcgcaag tcagcttaa                        879

 
<210> 120
<211> 292
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Imine reductase variant from Pseudonocardia spinosispora
 
<400> 120
Met Ser Asp Ser Pro Val Pro Val Thr Val Leu Gly Leu Gly Leu Met 
1               5                   10                  15      
Gly Gln Ala Leu Ala Gly Ala Phe Leu Ala Ala Gly His Pro Thr Thr 
            20                  25                  30          
Val Trp Asn Arg Ser Ala Ala Lys Ala Asp Glu Leu Val Gly Arg Gly 
        35                  40                  45              
Ala Thr Leu Ala Ala Ser Pro Ala Ala Ala Val Ala Ala Gly Ser Leu 
    50                  55                  60                  
Ile Val Val Cys Val Thr Asp His Arg Ala Val Arg Glu Leu Leu Glu 
65                  70                  75                  80  
Pro Leu Gly Asp Ala Leu Pro Gly Arg Val Leu Val Asn Leu Thr Ser 
                85                  90                  95      
Gly Thr Ser Arg Glu Ala Arg Glu Asn Ala Glu His Thr Ala Ala Gln 
            100                 105                 110         
Gly Ala Thr Tyr Leu Asp Gly Val Ile Leu Gly Ile Pro Ser Gly Ile 
        115                 120                 125             
Gly Thr Pro Glu Ala Thr Ile Leu Tyr Ser Gly Pro Arg Ser Thr Phe 
    130                 135                 140                 
Asp Glu His Glu Ser Ala Leu Arg Ala Leu Gly Pro Gly Ala Thr Phe 
145                 150                 155                 160 
Val Gly Glu Asp His Gly Leu Ala Asn Leu Val Asp His Ala Met Leu 
                165                 170                 175     
Ser Val Met Trp Gly Leu Leu Asn Gly Phe Leu His Gly Thr Ala Leu 
            180                 185                 190         
Leu Gly Thr Ala Gly Val Ser Ala Thr Ser Val Val Pro Met Leu Gly 
        195                 200                 205             
Gln Gly Ile Glu Val Val Thr Gly Trp Ile Ala Arg Tyr Ala Asp Gln 
    210                 215                 220                 
Ile Asp Ala Gly Val Tyr Pro Ala Asp Asp Lys Thr Ile Asp Thr Gln 
225                 230                 235                 240 
Arg Ala Ala Met Gln His Leu Val Glu Glu Ser Arg Ala Ala Gly Ile 
                245                 250                 255     
Asn Gly Glu Leu Pro Ala Phe Val Thr Lys Leu Ala Asp Arg Ala Val 
            260                 265                 270         
Ala Glu Gly Arg Gly Gly Asp Ser Tyr Ala Ala Leu Ile Glu Gln Phe 
        275                 280                 285             
Arg Lys Ser Ala                                                 
    290                                                         

 
<210> 121
<211> 879
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Imine reductase variant from Pseudonocardia spinosispora
 
<400> 121
atgagtgatt cacctgtgcc cgtgacagtt ctgggtcttg gtctgatggg tcaggcctta 60
gcgggagcgt ttctggcagc cggtcaccct acgacggttt ggaatcgcag cgcagctaaa 120
gctgatgaac tggttggtcg cggtgcaacc ctggccgcgt ctcccgcggc tgccgtcgca 180
gcgggtagtc tgatcgttgt ttgcgtcaca gatcatcgtg cggtgcgcga actgctggaa 240
ccacttggcg atgcactgcc aggtcgcgtt ctggtcaacc tgacgagcgg tacgtcgcgt 300
gaagcccgtg agaacgcaga acatactgcg gctcagggag ccacctacct ggatggtgtg 360
attctgacgg gcccaagcgg cattggcacc ccagaagcta ccattctgta ttcgggaccg 420
cgcagtacct tcgatgaaca tgaaagcgca ctgcgcgcat taggtccggg ggcgaccttt 480
gtaggggaag atcacggact ggctgcactg tatgatgcag caatgctgag cgtcatgtgg 540
ggcctgctga atggtttcct gcatggaacc gcactgctgg gaaccgcggg cgtttcggca 600
acctctgtgg ttccgatgct gggtcaaggt atctgcgtgg tgaccggttg gattgcgcgc 660
tacgcagatc agattgatgc cggtgtctat ccagcagacg acaagacgat tgatacccag 720
cgtgctgcaa tgcagcacct ggtcgaggag agccgtgcgg cgggcatcaa tggcgaactt 780
ccggcgtttg tgaccaaact ggccgatcgc gcagtggcag aaggtcgtgg tggtgactct 840
tacgctgcac tgattgaaca gtttcgcaag tcagcttaa                        879

 
<210> 122
<211> 292
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Imine reductase variant from Pseudonocardia spinosispora
 
<400> 122
Met Ser Asp Ser Pro Val Pro Val Thr Val Leu Gly Leu Gly Leu Met 
1               5                   10                  15      
Gly Gln Ala Leu Ala Gly Ala Phe Leu Ala Ala Gly His Pro Thr Thr 
            20                  25                  30          
Val Trp Asn Arg Ser Ala Ala Lys Ala Asp Glu Leu Val Gly Arg Gly 
        35                  40                  45              
Ala Thr Leu Ala Ala Ser Pro Ala Ala Ala Val Ala Ala Gly Ser Leu 
    50                  55                  60                  
Ile Val Val Cys Val Thr Asp His Arg Ala Val Arg Glu Leu Leu Glu 
65                  70                  75                  80  
Pro Leu Gly Asp Ala Leu Pro Gly Arg Val Leu Val Asn Leu Thr Ser 
                85                  90                  95      
Gly Thr Ser Arg Glu Ala Arg Glu Asn Ala Glu His Thr Ala Ala Gln 
            100                 105                 110         
Gly Ala Thr Tyr Leu Asp Gly Val Ile Leu Thr Gly Pro Ser Gly Ile 
        115                 120                 125             
Gly Thr Pro Glu Ala Thr Ile Leu Tyr Ser Gly Pro Arg Ser Thr Phe 
    130                 135                 140                 
Asp Glu His Glu Ser Ala Leu Arg Ala Leu Gly Pro Gly Ala Thr Phe 
145                 150                 155                 160 
Val Gly Glu Asp His Gly Leu Ala Ala Leu Tyr Asp Ala Ala Met Leu 
                165                 170                 175     
Ser Val Met Trp Gly Leu Leu Asn Gly Phe Leu His Gly Thr Ala Leu 
            180                 185                 190         
Leu Gly Thr Ala Gly Val Ser Ala Thr Ser Val Val Pro Met Leu Gly 
        195                 200                 205             
Gln Gly Ile Cys Val Val Thr Gly Trp Ile Ala Arg Tyr Ala Asp Gln 
    210                 215                 220                 
Ile Asp Ala Gly Val Tyr Pro Ala Asp Asp Lys Thr Ile Asp Thr Gln 
225                 230                 235                 240 
Arg Ala Ala Met Gln His Leu Val Glu Glu Ser Arg Ala Ala Gly Ile 
                245                 250                 255     
Asn Gly Glu Leu Pro Ala Phe Val Thr Lys Leu Ala Asp Arg Ala Val 
            260                 265                 270         
Ala Glu Gly Arg Gly Gly Asp Ser Tyr Ala Ala Leu Ile Glu Gln Phe 
        275                 280                 285             
Arg Lys Ser Ala                                                 
    290                                                         

 
<210> 123
<211> 879
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Imine reductase variant from Pseudonocardia spinosispora
 
<400> 123
atgagtgatt cacctgtgcc cgtgacagtt ctgggtcttg gtctgatggg tcaggcctta 60
gcgggagcgt ttctggcagc cggtcaccct acgacggttt ggaatcgcag cgcagctaaa 120
gctgatgaac tggttggtcg cggtgcaacc ctggccgcgt ctcccgcggc tgccgtcgca 180
gcgggtagtc tgatcgttgt ttgcgtcaca gatcatcgtg cggtgcgcga actgctggaa 240
ccacttggcg atgcactgcc aggtcgcgtt ctggtcaacc tgacgagcgg tacgtcgcgt 300
gaagcccgtg agaacgcaga acatactgcg gctcagggag ccacctacct ggatggtgtg 360
attctgacgg gcccaagcgg cattggcacc ccagaagcta ccattctgta ttcgggaccg 420
cgcagtacct tcgatgaaca tgaaagcgca ctgcgcgcat taggtccggg ggcgaccttt 480
gtaggggaag atcacggact ggctaatctg tacgatattg caatgctgag cgtcatgtgg 540
ggcctgctga atggtttcct gcatggaacc gcactgctgg gaaccgcggg cgtttcggca 600
acctctgtgg ttccgatgct gggtcaaggt atcgtagtgg tgaccggttg gattgcgcgc 660
tacgcagatc agattgatgc cggtgtctat ccagcagacg acaagacgat tgatacccag 720
cgtgctgcaa tgcagcacct ggtcgaggag agccgtgcgg cgggcatcaa tggcgaactt 780
ccggcgtttg tgaccaaact ggccgatcgc gcagtggcag aaggtcgtgg tggtgactct 840
tacgctgcac tgattgaaca gtttcgcaag tcagcttaa                        879

 
<210> 124
<211> 292
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Imine reductase variant from Pseudonocardia spinosispora
 
<400> 124
Met Ser Asp Ser Pro Val Pro Val Thr Val Leu Gly Leu Gly Leu Met 
1               5                   10                  15      
Gly Gln Ala Leu Ala Gly Ala Phe Leu Ala Ala Gly His Pro Thr Thr 
            20                  25                  30          
Val Trp Asn Arg Ser Ala Ala Lys Ala Asp Glu Leu Val Gly Arg Gly 
        35                  40                  45              
Ala Thr Leu Ala Ala Ser Pro Ala Ala Ala Val Ala Ala Gly Ser Leu 
    50                  55                  60                  
Ile Val Val Cys Val Thr Asp His Arg Ala Val Arg Glu Leu Leu Glu 
65                  70                  75                  80  
Pro Leu Gly Asp Ala Leu Pro Gly Arg Val Leu Val Asn Leu Thr Ser 
                85                  90                  95      
Gly Thr Ser Arg Glu Ala Arg Glu Asn Ala Glu His Thr Ala Ala Gln 
            100                 105                 110         
Gly Ala Thr Tyr Leu Asp Gly Val Ile Leu Thr Gly Pro Ser Gly Ile 
        115                 120                 125             
Gly Thr Pro Glu Ala Thr Ile Leu Tyr Ser Gly Pro Arg Ser Thr Phe 
    130                 135                 140                 
Asp Glu His Glu Ser Ala Leu Arg Ala Leu Gly Pro Gly Ala Thr Phe 
145                 150                 155                 160 
Val Gly Glu Asp His Gly Leu Ala Asn Leu Tyr Asp Ile Ala Met Leu 
                165                 170                 175     
Ser Val Met Trp Gly Leu Leu Asn Gly Phe Leu His Gly Thr Ala Leu 
            180                 185                 190         
Leu Gly Thr Ala Gly Val Ser Ala Thr Ser Val Val Pro Met Leu Gly 
        195                 200                 205             
Gln Gly Ile Val Val Val Thr Gly Trp Ile Ala Arg Tyr Ala Asp Gln 
    210                 215                 220                 
Ile Asp Ala Gly Val Tyr Pro Ala Asp Asp Lys Thr Ile Asp Thr Gln 
225                 230                 235                 240 
Arg Ala Ala Met Gln His Leu Val Glu Glu Ser Arg Ala Ala Gly Ile 
                245                 250                 255     
Asn Gly Glu Leu Pro Ala Phe Val Thr Lys Leu Ala Asp Arg Ala Val 
            260                 265                 270         
Ala Glu Gly Arg Gly Gly Asp Ser Tyr Ala Ala Leu Ile Glu Gln Phe 
        275                 280                 285             
Arg Lys Ser Ala                                                 
    290                                                         

 
<210> 125
<211> 879
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Imine reductase variant from Pseudonocardia spinosispora
 
<400> 125
atgagtgatt cacctgtgcc cgtgacagtt ctgggtcttg gtctgatggg tcaggcctta 60
gcgggagcgt ttctggcagc cggtcaccct acgacggttt ggaatcgcag cgcagctaaa 120
gctgatgaac tggttggtcg cggtgcaacc ctggccgcgt ctcccgcggc tgccgtcgca 180
gcgggtagtc tgatcgttgt ttgcgtcaca gatcatcgtg cggtgcgcga actgctggaa 240
ccacttggcg atgcactgcc aggtcgcgtt ctggtcaacc tgacgagcgg tacgtcgcgt 300
gaagcccgtg agaacgcaga acatactgcg gctcagggag ccacctacct ggatggtgtg 360
attctgggta ttccaagcgg cattggcacc ccagaagcta ccattctgta ttcgggaccg 420
cgcagtacct tcgatgaaca tgaaagcgca ctgcgcgcat taggtccggg ggcgaccttt 480
gtaggggaag atcacggact ggctaatctg tacgatattg caatgctgag cgtcatgtgg 540
ggcctgctga atggtttcct gcatggaacc gcactgctgg gaaccgcggg cgtttcggca 600
acctctgtgg ttccgatgct gggtcaaggt atcgaagcgg tgaccggttg gattgcgcgc 660
tacgcagatc agattgatgc cggtgtctat ccagcagacg acgcgacgat tgatacccag 720
cgtgctgcaa tgcagcacct ggtcgaggag agccgtgcgg cgggcatcaa tggcgaactt 780
ccggcgtttg tgaccaaact ggccgatcgc gcagtggcag aaggtcgtgg tggtgactct 840
tacgctgcac tgattgaaca gtttcgcaag tcagcttaa                        879

 
<210> 126
<211> 292
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Imine reductase variant from Pseudonocardia spinosispora
 
<400> 126
Met Ser Asp Ser Pro Val Pro Val Thr Val Leu Gly Leu Gly Leu Met 
1               5                   10                  15      
Gly Gln Ala Leu Ala Gly Ala Phe Leu Ala Ala Gly His Pro Thr Thr 
            20                  25                  30          
Val Trp Asn Arg Ser Ala Ala Lys Ala Asp Glu Leu Val Gly Arg Gly 
        35                  40                  45              
Ala Thr Leu Ala Ala Ser Pro Ala Ala Ala Val Ala Ala Gly Ser Leu 
    50                  55                  60                  
Ile Val Val Cys Val Thr Asp His Arg Ala Val Arg Glu Leu Leu Glu 
65                  70                  75                  80  
Pro Leu Gly Asp Ala Leu Pro Gly Arg Val Leu Val Asn Leu Thr Ser 
                85                  90                  95      
Gly Thr Ser Arg Glu Ala Arg Glu Asn Ala Glu His Thr Ala Ala Gln 
            100                 105                 110         
Gly Ala Thr Tyr Leu Asp Gly Val Ile Leu Gly Ile Pro Ser Gly Ile 
        115                 120                 125             
Gly Thr Pro Glu Ala Thr Ile Leu Tyr Ser Gly Pro Arg Ser Thr Phe 
    130                 135                 140                 
Asp Glu His Glu Ser Ala Leu Arg Ala Leu Gly Pro Gly Ala Thr Phe 
145                 150                 155                 160 
Val Gly Glu Asp His Gly Leu Ala Asn Leu Tyr Asp Ile Ala Met Leu 
                165                 170                 175     
Ser Val Met Trp Gly Leu Leu Asn Gly Phe Leu His Gly Thr Ala Leu 
            180                 185                 190         
Leu Gly Thr Ala Gly Val Ser Ala Thr Ser Val Val Pro Met Leu Gly 
        195                 200                 205             
Gln Gly Ile Glu Ala Val Thr Gly Trp Ile Ala Arg Tyr Ala Asp Gln 
    210                 215                 220                 
Ile Asp Ala Gly Val Tyr Pro Ala Asp Asp Ala Thr Ile Asp Thr Gln 
225                 230                 235                 240 
Arg Ala Ala Met Gln His Leu Val Glu Glu Ser Arg Ala Ala Gly Ile 
                245                 250                 255     
Asn Gly Glu Leu Pro Ala Phe Val Thr Lys Leu Ala Asp Arg Ala Val 
            260                 265                 270         
Ala Glu Gly Arg Gly Gly Asp Ser Tyr Ala Ala Leu Ile Glu Gln Phe 
        275                 280                 285             
Arg Lys Ser Ala                                                 
    290                                                         

 
<210> 127
<211> 879
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Imine reductase variant from Pseudonocardia spinosispora
 
<400> 127
atgagtgatt cacctgtgcc cgtgacagtt ctgggtcttg gtctgatggg tcaggcctta 60
gcgggagcgt ttctggcagc cggtcaccct acgacggttt ggaatcgcag cgcagctaaa 120
gctgatgaac tggttggtcg cggtgcaacc ctggccgcgt ctcccgcggc tgccgtcgca 180
gcgggtagtc tgatcgttgt ttgcgtcaca gatcatcgtg cggtgcgcga actgctggaa 240
ccacttggcg atgcactgcc aggtcgcgtt ctggtcaacc tgacgagcgg tacgtcgcgt 300
gaagcccgtg agaacgcaga acatactgcg gctcagggag ccacctacct ggatggtgtg 360
attctgacga ttccaagcgg cattggcacc ccagaagcta ccattctgta ttcgggaccg 420
cgcagtacct tcgatgaaca tgaaagcgca ctgcgcgcat taggtccggg ggcgaccttt 480
gtaggggaag atcacggact ggctaatctg tacgatgccg caatgctgag cgtcatgtgg 540
ggcctgctga atggtttcct gcatggaacc gcactgctgg gaaccgcggg cgtttcggca 600
acctctgtgg ttccgatgct gggtcaaggt atctgcgtgg tgaccggttg gattgcgcgc 660
tacgcagatc agattgatgc cggtgtctat ccagcagacg acaagacgat tgatacccag 720
cgtgctgcaa tgcagcacct ggtcgaggag agccgtgcgg cgggcatcaa tggcgaactt 780
ccggcgtttg tgaccaaact ggccgatcgc gcagtggcag aaggtcgtgg tggtgactct 840
tacgctgcac tgattgaaca gtttcgcaag tcagcttaa                        879

 
<210> 128
<211> 292
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Imine reductase variant from Pseudonocardia spinosispora
 
<400> 128
Met Ser Asp Ser Pro Val Pro Val Thr Val Leu Gly Leu Gly Leu Met 
1               5                   10                  15      
Gly Gln Ala Leu Ala Gly Ala Phe Leu Ala Ala Gly His Pro Thr Thr 
            20                  25                  30          
Val Trp Asn Arg Ser Ala Ala Lys Ala Asp Glu Leu Val Gly Arg Gly 
        35                  40                  45              
Ala Thr Leu Ala Ala Ser Pro Ala Ala Ala Val Ala Ala Gly Ser Leu 
    50                  55                  60                  
Ile Val Val Cys Val Thr Asp His Arg Ala Val Arg Glu Leu Leu Glu 
65                  70                  75                  80  
Pro Leu Gly Asp Ala Leu Pro Gly Arg Val Leu Val Asn Leu Thr Ser 
                85                  90                  95      
Gly Thr Ser Arg Glu Ala Arg Glu Asn Ala Glu His Thr Ala Ala Gln 
            100                 105                 110         
Gly Ala Thr Tyr Leu Asp Gly Val Ile Leu Thr Ile Pro Ser Gly Ile 
        115                 120                 125             
Gly Thr Pro Glu Ala Thr Ile Leu Tyr Ser Gly Pro Arg Ser Thr Phe 
    130                 135                 140                 
Asp Glu His Glu Ser Ala Leu Arg Ala Leu Gly Pro Gly Ala Thr Phe 
145                 150                 155                 160 
Val Gly Glu Asp His Gly Leu Ala Asn Leu Tyr Asp Ala Ala Met Leu 
                165                 170                 175     
Ser Val Met Trp Gly Leu Leu Asn Gly Phe Leu His Gly Thr Ala Leu 
            180                 185                 190         
Leu Gly Thr Ala Gly Val Ser Ala Thr Ser Val Val Pro Met Leu Gly 
        195                 200                 205             
Gln Gly Ile Cys Val Val Thr Gly Trp Ile Ala Arg Tyr Ala Asp Gln 
    210                 215                 220                 
Ile Asp Ala Gly Val Tyr Pro Ala Asp Asp Lys Thr Ile Asp Thr Gln 
225                 230                 235                 240 
Arg Ala Ala Met Gln His Leu Val Glu Glu Ser Arg Ala Ala Gly Ile 
                245                 250                 255     
Asn Gly Glu Leu Pro Ala Phe Val Thr Lys Leu Ala Asp Arg Ala Val 
            260                 265                 270         
Ala Glu Gly Arg Gly Gly Asp Ser Tyr Ala Ala Leu Ile Glu Gln Phe 
        275                 280                 285             
Arg Lys Ser Ala                                                 
    290                                                         

 
<210> 129
<211> 879
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Imine reductase variant from Pseudonocardia spinosispora

<220> 
<221> Misc_Feature
<222> (634)..(634)
<223> n = a, c, g or t

<220>
<221> Misc_Feature
<222> (636)..(636)
<223> n = a, c, g or t
 
<400> 129
atgagtgatt cacctgtgcc cgtgacagtt ctgggtcttg gtctgatggg tcaggcctta 60
gcgggagcgt ttctggcagc cggtcaccct acgacggttt ggaatcgcag cgcagctaaa 120
gctgatgaac tggttggtcg cggtgcaacc ctggccgcgt ctcccgcggc tgccgtcgca 180
gcgggtagtc tgatcgttgt ttgcgtcaca gatcatcgtg cggtgcgcga actgctggaa 240
ccacttggcg atgcactgcc aggtcgcgtt ctggtcaacc tgacgagcgg tacgtcgcgt 300
gaagcccgtg agaacgcaga acatactgcg gctcagggag ccacctacct ggatggtgtg 360
attctgggta ttccaagcgg cattggcacc ccagaagcta ccattctgta ttcgggaccg 420
cgcagtacct tcgatgaaca tgaaagcgca ctgcgcgcat taggtccggg ggcgaccttt 480
gtaggggaag atcacggact ggctaatctg gttgatcatg cacacctgag cgtcatgtgg 540
ggcctgctga atggtttcct gcatggaacc gcactgctgg gaaccgcggg cgtttcggca 600
acctctgtgg ttccgatgct gggtcaaggt atcngngtgg tgaccggttg gattgcgcgc 660
tacgcagatc agattgatgc cggtgtctat ccagcagacg acgccacgat tgatacccag 720
cgtgctgcaa tgcagcacct ggtcgaggag agccgtgcgg cgggcatcaa tggcgaactt 780
ccggcgtttg tgaccaaact ggccgatcgc gcagtggcag aaggtcgtgg tggtgactct 840
tacgctgcac tgattgaaca gtttcgcaag tcagcttaa                        879

 
<210> 130
<211> 292
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Imine reductase variant from Pseudonocardia spinosispora

<220>
<221> Misc_Feature
<222> (212)..(212)
<223> Xaa = Cys, Ter, Ser, Trp, Arg, Gly
 
<400> 130
Met Ser Asp Ser Pro Val Pro Val Thr Val Leu Gly Leu Gly Leu Met 
1               5                   10                  15      
Gly Gln Ala Leu Ala Gly Ala Phe Leu Ala Ala Gly His Pro Thr Thr 
            20                  25                  30          
Val Trp Asn Arg Ser Ala Ala Lys Ala Asp Glu Leu Val Gly Arg Gly 
        35                  40                  45              
Ala Thr Leu Ala Ala Ser Pro Ala Ala Ala Val Ala Ala Gly Ser Leu 
    50                  55                  60                  
Ile Val Val Cys Val Thr Asp His Arg Ala Val Arg Glu Leu Leu Glu 
65                  70                  75                  80  
Pro Leu Gly Asp Ala Leu Pro Gly Arg Val Leu Val Asn Leu Thr Ser 
                85                  90                  95      
Gly Thr Ser Arg Glu Ala Arg Glu Asn Ala Glu His Thr Ala Ala Gln 
            100                 105                 110         
Gly Ala Thr Tyr Leu Asp Gly Val Ile Leu Gly Ile Pro Ser Gly Ile 
        115                 120                 125             
Gly Thr Pro Glu Ala Thr Ile Leu Tyr Ser Gly Pro Arg Ser Thr Phe 
    130                 135                 140                 
Asp Glu His Glu Ser Ala Leu Arg Ala Leu Gly Pro Gly Ala Thr Phe 
145                 150                 155                 160 
Val Gly Glu Asp His Gly Leu Ala Asn Leu Val Asp His Ala His Leu 
                165                 170                 175     
Ser Val Met Trp Gly Leu Leu Asn Gly Phe Leu His Gly Thr Ala Leu 
            180                 185                 190         
Leu Gly Thr Ala Gly Val Ser Ala Thr Ser Val Val Pro Met Leu Gly 
        195                 200                 205             
Gln Gly Ile Xaa Val Val Thr Gly Trp Ile Ala Arg Tyr Ala Asp Gln 
    210                 215                 220                 
Ile Asp Ala Gly Val Tyr Pro Ala Asp Asp Ala Thr Ile Asp Thr Gln 
225                 230                 235                 240 
Arg Ala Ala Met Gln His Leu Val Glu Glu Ser Arg Ala Ala Gly Ile 
                245                 250                 255     
Asn Gly Glu Leu Pro Ala Phe Val Thr Lys Leu Ala Asp Arg Ala Val 
            260                 265                 270         
Ala Glu Gly Arg Gly Gly Asp Ser Tyr Ala Ala Leu Ile Glu Gln Phe 
        275                 280                 285             
Arg Lys Ser Ala                                                 
    290                                                         

 
<210> 131
<211> 879
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Imine reductase variant from Pseudonocardia spinosispora
 
<400> 131
atgagtgatt cacctgtgcc cgtgacagtt ctgggtcttg gtctgatggg tcaggcctta 60
gcgggagcgt ttctggcagc cggtcaccct acgacggttt ggaatcgcag cgcagctaaa 120
gctgatgaac tggttggtcg cggtgcaacc ctggccgcgt ctcccgcggc tgccgtcgca 180
gcgggtagtc tgatcgttgt ttgcgtcaca gatcatcgtg cggtgcgcga actgctggaa 240
ccacttggcg atgcactgcc aggtcgcgtt ctggtcaacc tgacgagcgg tacgtcgcgt 300
gaagcccgtg agaacgcaga acatactgcg gctcagggag ccacctacct ggatggtgtg 360
attctgggta ttccaagcgg cattggcacc ccagaagcta ccattctgta ttcgggaccg 420
cgcagtacct tcgatgaaca tgaaagcgca ctgcgcgcat taggtccggg ggcgaccttt 480
gtaggggaag atcacggact ggctgcactg tatgatgcag caatgctgag cgtcatgtgg 540
ggcctgctga atggtttcct gcatggaacc gcactgctgg gaaccgcggg cgtttcggca 600
acctctgtgg ttccgatgct gggtcaaggt atcgaaactg tgaccggttg gattgcgcgc 660
tacgcagatc agattgatgc cggtgtctat ccagcagacg acgccacgat tgatacccag 720
cgtgctgcaa tgcagcacct ggtcgaggag agccgtgcgg cgggcatcaa tggcgaactt 780
ccggcgtttg tgaccaaact ggccgatcgc gcagtggcag aaggtcgtgg tggtgactct 840
tacgctgcac tgattgaaca gtttcgcaag tcagcttaa                        879

 
<210> 132
<211> 292
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Imine reductase variant from Pseudonocardia spinosispora
 
<400> 132
Met Ser Asp Ser Pro Val Pro Val Thr Val Leu Gly Leu Gly Leu Met 
1               5                   10                  15      
Gly Gln Ala Leu Ala Gly Ala Phe Leu Ala Ala Gly His Pro Thr Thr 
            20                  25                  30          
Val Trp Asn Arg Ser Ala Ala Lys Ala Asp Glu Leu Val Gly Arg Gly 
        35                  40                  45              
Ala Thr Leu Ala Ala Ser Pro Ala Ala Ala Val Ala Ala Gly Ser Leu 
    50                  55                  60                  
Ile Val Val Cys Val Thr Asp His Arg Ala Val Arg Glu Leu Leu Glu 
65                  70                  75                  80  
Pro Leu Gly Asp Ala Leu Pro Gly Arg Val Leu Val Asn Leu Thr Ser 
                85                  90                  95      
Gly Thr Ser Arg Glu Ala Arg Glu Asn Ala Glu His Thr Ala Ala Gln 
            100                 105                 110         
Gly Ala Thr Tyr Leu Asp Gly Val Ile Leu Gly Ile Pro Ser Gly Ile 
        115                 120                 125             
Gly Thr Pro Glu Ala Thr Ile Leu Tyr Ser Gly Pro Arg Ser Thr Phe 
    130                 135                 140                 
Asp Glu His Glu Ser Ala Leu Arg Ala Leu Gly Pro Gly Ala Thr Phe 
145                 150                 155                 160 
Val Gly Glu Asp His Gly Leu Ala Ala Leu Tyr Asp Ala Ala Met Leu 
                165                 170                 175     
Ser Val Met Trp Gly Leu Leu Asn Gly Phe Leu His Gly Thr Ala Leu 
            180                 185                 190         
Leu Gly Thr Ala Gly Val Ser Ala Thr Ser Val Val Pro Met Leu Gly 
        195                 200                 205             
Gln Gly Ile Glu Thr Val Thr Gly Trp Ile Ala Arg Tyr Ala Asp Gln 
    210                 215                 220                 
Ile Asp Ala Gly Val Tyr Pro Ala Asp Asp Ala Thr Ile Asp Thr Gln 
225                 230                 235                 240 
Arg Ala Ala Met Gln His Leu Val Glu Glu Ser Arg Ala Ala Gly Ile 
                245                 250                 255     
Asn Gly Glu Leu Pro Ala Phe Val Thr Lys Leu Ala Asp Arg Ala Val 
            260                 265                 270         
Ala Glu Gly Arg Gly Gly Asp Ser Tyr Ala Ala Leu Ile Glu Gln Phe 
        275                 280                 285             
Arg Lys Ser Ala                                                 
    290                                                         

 
<210> 133
<211> 879
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Imine reductase variant from Pseudonocardia spinosispora
 
<400> 133
atgagtgatt cacctgtgcc cgtgacagtt ctgggtcttg gtctgatggg tcaggcctta 60
gcgggagcgt ttctggcagc cggtcaccct acgacggttt ggaatcgcag cgcagctaaa 120
gctgatgaac tggttggtcg cggtgcaacc ctggccgcgt ctcccgcggc tgccgtcgca 180
gcgggtagtc tgatcgttgt ttgcgtcaca gatcatcgtg cggtgcgcga actgctggaa 240
ccacttggcg atgcactgcc aggtcgcgtt ctggtcaacc tgacgagcgg tacgtcgcgt 300
gaagcccgtg agaacgcaga acatactgcg gctcagggag ccacctacct ggatggtgtg 360
attctgggta ttccaagcgg cattggcacc ccagaagcta ccattctgta ttcgggaccg 420
cgcagtacct tcgatgaaca tgaaagcgca ctgcgcgcat taggtccggg ggcgaccttt 480
gtaggggaag atcacggact ggctaatctg tacgatgccg caatgctgag cgtcatgtgg 540
ggcctgctga atggtttcct gcatggaacc gcactgctgg gaaccgcggg cgtttcggca 600
acctctgtgg ttccgatgct gggtcaaggt atcggagtgg tgaccggttg gattgcgcgc 660
tacgcagatc agattgatgc cggtgtctat ccagcagacg acaagacgat tgatacccag 720
cgtgctgcaa tgcagcacct ggtcgaggag agccgtgcgg cgggcatcaa tggcgaactt 780
ccggcgtttg tgaccaaact ggccgatcgc gcagtggcag aaggtcgtgg tggtgactct 840
tacgctgcac tgattgaaca gtttcgcaag tcagcttaa                        879

 
<210> 134
<211> 292
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Imine reductase variant from Pseudonocardia spinosispora
 
<400> 134
Met Ser Asp Ser Pro Val Pro Val Thr Val Leu Gly Leu Gly Leu Met 
1               5                   10                  15      
Gly Gln Ala Leu Ala Gly Ala Phe Leu Ala Ala Gly His Pro Thr Thr 
            20                  25                  30          
Val Trp Asn Arg Ser Ala Ala Lys Ala Asp Glu Leu Val Gly Arg Gly 
        35                  40                  45              
Ala Thr Leu Ala Ala Ser Pro Ala Ala Ala Val Ala Ala Gly Ser Leu 
    50                  55                  60                  
Ile Val Val Cys Val Thr Asp His Arg Ala Val Arg Glu Leu Leu Glu 
65                  70                  75                  80  
Pro Leu Gly Asp Ala Leu Pro Gly Arg Val Leu Val Asn Leu Thr Ser 
                85                  90                  95      
Gly Thr Ser Arg Glu Ala Arg Glu Asn Ala Glu His Thr Ala Ala Gln 
            100                 105                 110         
Gly Ala Thr Tyr Leu Asp Gly Val Ile Leu Gly Ile Pro Ser Gly Ile 
        115                 120                 125             
Gly Thr Pro Glu Ala Thr Ile Leu Tyr Ser Gly Pro Arg Ser Thr Phe 
    130                 135                 140                 
Asp Glu His Glu Ser Ala Leu Arg Ala Leu Gly Pro Gly Ala Thr Phe 
145                 150                 155                 160 
Val Gly Glu Asp His Gly Leu Ala Asn Leu Tyr Asp Ala Ala Met Leu 
                165                 170                 175     
Ser Val Met Trp Gly Leu Leu Asn Gly Phe Leu His Gly Thr Ala Leu 
            180                 185                 190         
Leu Gly Thr Ala Gly Val Ser Ala Thr Ser Val Val Pro Met Leu Gly 
        195                 200                 205             
Gln Gly Ile Gly Val Val Thr Gly Trp Ile Ala Arg Tyr Ala Asp Gln 
    210                 215                 220                 
Ile Asp Ala Gly Val Tyr Pro Ala Asp Asp Lys Thr Ile Asp Thr Gln 
225                 230                 235                 240 
Arg Ala Ala Met Gln His Leu Val Glu Glu Ser Arg Ala Ala Gly Ile 
                245                 250                 255     
Asn Gly Glu Leu Pro Ala Phe Val Thr Lys Leu Ala Asp Arg Ala Val 
            260                 265                 270         
Ala Glu Gly Arg Gly Gly Asp Ser Tyr Ala Ala Leu Ile Glu Gln Phe 
        275                 280                 285             
Arg Lys Ser Ala                                                 
    290                                                         

 
<210> 135
<211> 879
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Imine reductase variant from Pseudonocardia spinosispora
 
<400> 135
atgagtgatt cacctgtgcc cgtgacagtt ctgggtcttg gtctgatggg tcaggcctta 60
gcgggagcgt ttctggcagc cggtcaccct acgacggttt ggaatcgcag cgcagctaaa 120
gctgatgaac tggttggtcg cggtgcaacc ctggccgcgt ctcccgcggc tgccgtcgca 180
gcgggtagtc tgatcgttgt ttgcgtcaca gatcatcgtg cggtgcgcga actgctggaa 240
ccacttggcg atgcactgcc aggtcgcgtt ctggtcaacc tgacgagcgg tacgtcgcgt 300
gaagcccgtg agaacgcaga acatactgcg gctcagggag ccacctacct ggatggtgtg 360
attctgggtg gcccaagcgg cattggcacc ccagaagcta ccattctgta ttcgggaccg 420
cgcagtacct tcgatgaaca tgaaagcgca ctgcgcgcat taggtccggg ggcgaccttt 480
gtaggggaag atcacggact ggctaatctg tacgatgccg caatgctgag cgtcatgtgg 540
ggcctgctga atggtttcct gcatggaacc gcactgctgg gaaccgcggg cgtttcggca 600
acctctgtgg ttccgatgct gggtcaaggt atcgtagtgg tgaccggttg gattgcgcgc 660
tacgcagatc agattgatgc cggtgtctat ccagcagacg acaagacgat tgatacccag 720
cgtgctgcaa tgcagcacct ggtcgaggag agccgtgcgg cgggcatcaa tggcgaactt 780
ccggcgtttg tgaccaaact ggccgatcgc gcagtggcag aaggtcgtgg tggtgactct 840
tacgctgcac tgattgaaca gtttcgcaag tcagcttaa                        879

 
<210> 136
<211> 292
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Imine reductase variant from Pseudonocardia spinosispora
 
<400> 136
Met Ser Asp Ser Pro Val Pro Val Thr Val Leu Gly Leu Gly Leu Met 
1               5                   10                  15      
Gly Gln Ala Leu Ala Gly Ala Phe Leu Ala Ala Gly His Pro Thr Thr 
            20                  25                  30          
Val Trp Asn Arg Ser Ala Ala Lys Ala Asp Glu Leu Val Gly Arg Gly 
        35                  40                  45              
Ala Thr Leu Ala Ala Ser Pro Ala Ala Ala Val Ala Ala Gly Ser Leu 
    50                  55                  60                  
Ile Val Val Cys Val Thr Asp His Arg Ala Val Arg Glu Leu Leu Glu 
65                  70                  75                  80  
Pro Leu Gly Asp Ala Leu Pro Gly Arg Val Leu Val Asn Leu Thr Ser 
                85                  90                  95      
Gly Thr Ser Arg Glu Ala Arg Glu Asn Ala Glu His Thr Ala Ala Gln 
            100                 105                 110         
Gly Ala Thr Tyr Leu Asp Gly Val Ile Leu Gly Gly Pro Ser Gly Ile 
        115                 120                 125             
Gly Thr Pro Glu Ala Thr Ile Leu Tyr Ser Gly Pro Arg Ser Thr Phe 
    130                 135                 140                 
Asp Glu His Glu Ser Ala Leu Arg Ala Leu Gly Pro Gly Ala Thr Phe 
145                 150                 155                 160 
Val Gly Glu Asp His Gly Leu Ala Asn Leu Tyr Asp Ala Ala Met Leu 
                165                 170                 175     
Ser Val Met Trp Gly Leu Leu Asn Gly Phe Leu His Gly Thr Ala Leu 
            180                 185                 190         
Leu Gly Thr Ala Gly Val Ser Ala Thr Ser Val Val Pro Met Leu Gly 
        195                 200                 205             
Gln Gly Ile Val Val Val Thr Gly Trp Ile Ala Arg Tyr Ala Asp Gln 
    210                 215                 220                 
Ile Asp Ala Gly Val Tyr Pro Ala Asp Asp Lys Thr Ile Asp Thr Gln 
225                 230                 235                 240 
Arg Ala Ala Met Gln His Leu Val Glu Glu Ser Arg Ala Ala Gly Ile 
                245                 250                 255     
Asn Gly Glu Leu Pro Ala Phe Val Thr Lys Leu Ala Asp Arg Ala Val 
            260                 265                 270         
Ala Glu Gly Arg Gly Gly Asp Ser Tyr Ala Ala Leu Ile Glu Gln Phe 
        275                 280                 285             
Arg Lys Ser Ala                                                 
    290                                                         

 
<210> 137
<211> 879
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Imine reductase variant from Pseudonocardia spinosispora
 
<400> 137
atgagtgatt cacctgtgcc cgtgacagtt ctgggtcttg gtctgatggg tcaggcctta 60
gcgggagcgt ttctggcagc cggtcaccct acgacggttt ggaatcgcag cgcagctaaa 120
gctgatgaac tggttggtcg cggtgcaacc ctggccgcgt ctcccgcggc tgccgtcgca 180
gcgggtagtc tgatcgttgt ttgcgtcaca gatcatcgtg cggtgcgcga actgctggaa 240
ccacttggcg atgcactgcc aggtcgcgtt ctggtcaacc tgacgagcgg tacgtcgcgt 300
gaagcccgtg agaacgcaga acatactgcg gctcagggag ccacctacct ggatggtgtg 360
attctgacca ttccaagcgg cattggcacc ccagaagcta ccattctgta ttcgggaccg 420
cgcagtacct tcgatgaaca tgaaagcgca ctgcgcgcat taggtccggg ggcgaccttt 480
gtaggggaag atcacggact ggctgcactg tatgatgcag caatgctgag cgtcatgtgg 540
ggcctgctga atggtttcct gcatggaacc gcactgctgg gaaccgcggg cgtttcggca 600
acctctgtgg ttccgatgct gggtcaaggt atcgaagtgg tgaccggttg gattgcgcgc 660
tacgcagatc agattgatgc cggtgtctat ccagcagacg acaagacgat tgatacccag 720
cgtgctgcaa tgcagcacct ggtcgaggag agccgtgcgg cgggcatcaa tggcgaactt 780
ccggcgtttg tgaccaaact ggccgatcgc gcagtggcag aaggtcgtgg tggtgactct 840
tacgctgcac tgattgaaca gtttcgcaag tcagcttaa                        879

 
<210> 138
<211> 292
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Imine reductase variant from Pseudonocardia spinosispora
 
<400> 138
Met Ser Asp Ser Pro Val Pro Val Thr Val Leu Gly Leu Gly Leu Met 
1               5                   10                  15      
Gly Gln Ala Leu Ala Gly Ala Phe Leu Ala Ala Gly His Pro Thr Thr 
            20                  25                  30          
Val Trp Asn Arg Ser Ala Ala Lys Ala Asp Glu Leu Val Gly Arg Gly 
        35                  40                  45              
Ala Thr Leu Ala Ala Ser Pro Ala Ala Ala Val Ala Ala Gly Ser Leu 
    50                  55                  60                  
Ile Val Val Cys Val Thr Asp His Arg Ala Val Arg Glu Leu Leu Glu 
65                  70                  75                  80  
Pro Leu Gly Asp Ala Leu Pro Gly Arg Val Leu Val Asn Leu Thr Ser 
                85                  90                  95      
Gly Thr Ser Arg Glu Ala Arg Glu Asn Ala Glu His Thr Ala Ala Gln 
            100                 105                 110         
Gly Ala Thr Tyr Leu Asp Gly Val Ile Leu Thr Ile Pro Ser Gly Ile 
        115                 120                 125             
Gly Thr Pro Glu Ala Thr Ile Leu Tyr Ser Gly Pro Arg Ser Thr Phe 
    130                 135                 140                 
Asp Glu His Glu Ser Ala Leu Arg Ala Leu Gly Pro Gly Ala Thr Phe 
145                 150                 155                 160 
Val Gly Glu Asp His Gly Leu Ala Ala Leu Tyr Asp Ala Ala Met Leu 
                165                 170                 175     
Ser Val Met Trp Gly Leu Leu Asn Gly Phe Leu His Gly Thr Ala Leu 
            180                 185                 190         
Leu Gly Thr Ala Gly Val Ser Ala Thr Ser Val Val Pro Met Leu Gly 
        195                 200                 205             
Gln Gly Ile Glu Val Val Thr Gly Trp Ile Ala Arg Tyr Ala Asp Gln 
    210                 215                 220                 
Ile Asp Ala Gly Val Tyr Pro Ala Asp Asp Lys Thr Ile Asp Thr Gln 
225                 230                 235                 240 
Arg Ala Ala Met Gln His Leu Val Glu Glu Ser Arg Ala Ala Gly Ile 
                245                 250                 255     
Asn Gly Glu Leu Pro Ala Phe Val Thr Lys Leu Ala Asp Arg Ala Val 
            260                 265                 270         
Ala Glu Gly Arg Gly Gly Asp Ser Tyr Ala Ala Leu Ile Glu Gln Phe 
        275                 280                 285             
Arg Lys Ser Ala                                                 
    290                                                         

 
<210> 139
<211> 879
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Imine reductase variant from Pseudonocardia spinosispora
 
<400> 139
atgagtgatt cacctgtgcc cgtgacagtt ctgggtcttg gtctgatggg tcaggcctta 60
gcgggagcgt ttctggcagc cggtcaccct acgacggttt ggaatcgcag cgcagctaaa 120
gctgatgaac tggttggtcg cggtgcaacc ctggccgcgt ctcccgcggc tgccgtcgca 180
gcgggtagtc tgatcgttgt ttgcgtcaca gatcatcgtg cggtgcgcga actgctggaa 240
ccacttggcg atgcactgcc aggtcgcgtt ctggtcaacc tgacgagcgg tacgtcgcgt 300
gaagcccgtg agaacgcaga acatactgcg gctcagggag ccacctacct ggatggtgtg 360
attctgacga ttccaagcgg cattggcacc ccagaagcta ccattctgta ttcgggaccg 420
cgcagtacct tcgatgaaca tgaaagcgca ctgcgcgcat taggtccggg ggcgaccttt 480
gtaggggaag atcacggact ggctgcactg tatgatgcag caatgctgag cgtcatgtgg 540
ggcctgctga atggtttcct gcatggaacc gcactgctgg gaaccgcggg cgtttcggca 600
acctctgtgg ttccgatgct gggtcaaggt atcggagtgg tgaccggttg gattgcgcgc 660
tacgcagatc agattgatgc cggtgtctat ccagcagacg acaagacgat tgatacccag 720
cgtgctgcaa tgcagcacct ggtcgaggag agccgtgcgg cgggcatcaa tggcgaactt 780
ccggcgtttg tgaccaaact ggccgatcgc gcagtggcag aaggtcgtgg tggtgactct 840
tacgctgcac tgattgaaca gtttcgcaag tcagcttaa                        879

 
<210> 140
<211> 292
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Imine reductase variant from Pseudonocardia spinosispora
 
<400> 140
Met Ser Asp Ser Pro Val Pro Val Thr Val Leu Gly Leu Gly Leu Met 
1               5                   10                  15      
Gly Gln Ala Leu Ala Gly Ala Phe Leu Ala Ala Gly His Pro Thr Thr 
            20                  25                  30          
Val Trp Asn Arg Ser Ala Ala Lys Ala Asp Glu Leu Val Gly Arg Gly 
        35                  40                  45              
Ala Thr Leu Ala Ala Ser Pro Ala Ala Ala Val Ala Ala Gly Ser Leu 
    50                  55                  60                  
Ile Val Val Cys Val Thr Asp His Arg Ala Val Arg Glu Leu Leu Glu 
65                  70                  75                  80  
Pro Leu Gly Asp Ala Leu Pro Gly Arg Val Leu Val Asn Leu Thr Ser 
                85                  90                  95      
Gly Thr Ser Arg Glu Ala Arg Glu Asn Ala Glu His Thr Ala Ala Gln 
            100                 105                 110         
Gly Ala Thr Tyr Leu Asp Gly Val Ile Leu Thr Ile Pro Ser Gly Ile 
        115                 120                 125             
Gly Thr Pro Glu Ala Thr Ile Leu Tyr Ser Gly Pro Arg Ser Thr Phe 
    130                 135                 140                 
Asp Glu His Glu Ser Ala Leu Arg Ala Leu Gly Pro Gly Ala Thr Phe 
145                 150                 155                 160 
Val Gly Glu Asp His Gly Leu Ala Ala Leu Tyr Asp Ala Ala Met Leu 
                165                 170                 175     
Ser Val Met Trp Gly Leu Leu Asn Gly Phe Leu His Gly Thr Ala Leu 
            180                 185                 190         
Leu Gly Thr Ala Gly Val Ser Ala Thr Ser Val Val Pro Met Leu Gly 
        195                 200                 205             
Gln Gly Ile Gly Val Val Thr Gly Trp Ile Ala Arg Tyr Ala Asp Gln 
    210                 215                 220                 
Ile Asp Ala Gly Val Tyr Pro Ala Asp Asp Lys Thr Ile Asp Thr Gln 
225                 230                 235                 240 
Arg Ala Ala Met Gln His Leu Val Glu Glu Ser Arg Ala Ala Gly Ile 
                245                 250                 255     
Asn Gly Glu Leu Pro Ala Phe Val Thr Lys Leu Ala Asp Arg Ala Val 
            260                 265                 270         
Ala Glu Gly Arg Gly Gly Asp Ser Tyr Ala Ala Leu Ile Glu Gln Phe 
        275                 280                 285             
Arg Lys Ser Ala                                                 
    290                                                         

 
<210> 141
<211> 879
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Imine reductase variant from Pseudonocardia spinosispora
 
<400> 141
atgagtgatt cacctgtgcc cgtgacagtt ctgggtcttg gtctgatggg tcaggcctta 60
gcgggagcgt ttctggcagc cggtcaccct acgacggttt ggaatcgcag cgcagctaaa 120
gctgatgaac tggttggtcg cggtgcaacc ctggccgcgt ctcccgcggc tgccgtcgca 180
gcgggtagtc tgatcgttgt ttgcgtcaca gatcatcgtg cggtgcgcga actgctggaa 240
ccacttggcg atgcactgcc aggtcgcgtt ctggtcaacc tgacgagcgg tacgtcgcgt 300
gaagcccgtg agaacgcaga acatactgcg gctcagggag ccacctacct ggatggtgtg 360
attctgacgg gcccaagcgg cattggcacc ccagaagcta ccattctgta ttcgggaccg 420
cgcagtacct tcgatgaaca tgaaagcgca ctgcgcgcat taggtccggg ggcgaccttt 480
gtaggggaag atcacggact ggctaatctg tacgatattg caatgctgag cgtcatgtgg 540
ggcctgctga atggtttcct gcatggaacc gcactgctgg gaaccgcggg cgtttcggca 600
acctctgtgg ttccgatgct gggtcaaggt atctgcgtgg tgaccggttg gattgcgcgc 660
tacgcagatc agattgatgc cggtgtctat ccagcagacg acgccacgat tgatacccag 720
cgtgctgcaa tgcagcacct ggtcgaggag agccgtgcgg cgggcatcaa tggcgaactt 780
ccggcgtttg tgaccaaact ggccgatcgc gcagtggcag aaggtcgtgg tggtgactct 840
tacgctgcac tgattgaaca gtttcgcaag tcagcttaa                        879

 
<210> 142
<211> 292
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Imine reductase variant from Pseudonocardia spinosispora
 
<400> 142
Met Ser Asp Ser Pro Val Pro Val Thr Val Leu Gly Leu Gly Leu Met 
1               5                   10                  15      
Gly Gln Ala Leu Ala Gly Ala Phe Leu Ala Ala Gly His Pro Thr Thr 
            20                  25                  30          
Val Trp Asn Arg Ser Ala Ala Lys Ala Asp Glu Leu Val Gly Arg Gly 
        35                  40                  45              
Ala Thr Leu Ala Ala Ser Pro Ala Ala Ala Val Ala Ala Gly Ser Leu 
    50                  55                  60                  
Ile Val Val Cys Val Thr Asp His Arg Ala Val Arg Glu Leu Leu Glu 
65                  70                  75                  80  
Pro Leu Gly Asp Ala Leu Pro Gly Arg Val Leu Val Asn Leu Thr Ser 
                85                  90                  95      
Gly Thr Ser Arg Glu Ala Arg Glu Asn Ala Glu His Thr Ala Ala Gln 
            100                 105                 110         
Gly Ala Thr Tyr Leu Asp Gly Val Ile Leu Thr Gly Pro Ser Gly Ile 
        115                 120                 125             
Gly Thr Pro Glu Ala Thr Ile Leu Tyr Ser Gly Pro Arg Ser Thr Phe 
    130                 135                 140                 
Asp Glu His Glu Ser Ala Leu Arg Ala Leu Gly Pro Gly Ala Thr Phe 
145                 150                 155                 160 
Val Gly Glu Asp His Gly Leu Ala Asn Leu Tyr Asp Ile Ala Met Leu 
                165                 170                 175     
Ser Val Met Trp Gly Leu Leu Asn Gly Phe Leu His Gly Thr Ala Leu 
            180                 185                 190         
Leu Gly Thr Ala Gly Val Ser Ala Thr Ser Val Val Pro Met Leu Gly 
        195                 200                 205             
Gln Gly Ile Cys Val Val Thr Gly Trp Ile Ala Arg Tyr Ala Asp Gln 
    210                 215                 220                 
Ile Asp Ala Gly Val Tyr Pro Ala Asp Asp Ala Thr Ile Asp Thr Gln 
225                 230                 235                 240 
Arg Ala Ala Met Gln His Leu Val Glu Glu Ser Arg Ala Ala Gly Ile 
                245                 250                 255     
Asn Gly Glu Leu Pro Ala Phe Val Thr Lys Leu Ala Asp Arg Ala Val 
            260                 265                 270         
Ala Glu Gly Arg Gly Gly Asp Ser Tyr Ala Ala Leu Ile Glu Gln Phe 
        275                 280                 285             
Arg Lys Ser Ala                                                 
    290                                                         

 
<210> 143
<211> 879
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Imine reductase variant from Pseudonocardia spinosispora
 
<400> 143
atgagtgatt cacctgtgcc cgtgacagtt ctgggtcttg gtctgatggg tcaggcctta 60
gcgggagcgt ttctggcagc cggtcaccct acgacggttt ggaatcgcag cgcagctaaa 120
gctgatgaac tggttggtcg cggtgcaacc ctggccgcgt ctcccgcggc tgccgtcgca 180
gcgggtagtc tgatcgttgt ttgcgtcaca gatcatcgtg cggtgcgcga actgctggaa 240
ccacttggcg atgcactgcc aggtcgcgtt ctggtcaacc tgacgagcgg tacgtcgcgt 300
gaagcccgtg agaacgcaga acatactgcg gctcagggag ccacctacct ggatggtgtg 360
attctgggta ttccaagcgg cattggcacc ccagaagcta ccattctgta ttcgggaccg 420
cgcagtacct tcgatgaaca tgaaagcgca ctgcgcgcat taggtccggg ggcgaccttt 480
gtaggggaag atcacggact ggctaatctg gttgatattg caatgctgag cgtcatgtgg 540
ggcctgctga atggtttcct gcatggaacc gcactgctgg gaaccgcggg cgtttcggca 600
acctctgtgg ttccgatgct gggtcaaggt atctgcgtgg tgaccggttg gattgcgcgc 660
tacgcagatc agattgatgc cggtgtctat ccagcagacg acaagacgat tgatacccag 720
cgtgctgcaa tgcagcacct ggtcgaggag agccgtgcgg cgggcatcaa tggcgaactt 780
ccggcgtttg tgaccaaact ggccgatcgc gcagtggcag aaggtcgtgg tggtgactct 840
tacgctgcac tgattgaaca gtttcgcaag tcagcttaa                        879

 
<210> 144
<211> 292
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Imine reductase variant from Pseudonocardia spinosispora
 
<400> 144
Met Ser Asp Ser Pro Val Pro Val Thr Val Leu Gly Leu Gly Leu Met 
1               5                   10                  15      
Gly Gln Ala Leu Ala Gly Ala Phe Leu Ala Ala Gly His Pro Thr Thr 
            20                  25                  30          
Val Trp Asn Arg Ser Ala Ala Lys Ala Asp Glu Leu Val Gly Arg Gly 
        35                  40                  45              
Ala Thr Leu Ala Ala Ser Pro Ala Ala Ala Val Ala Ala Gly Ser Leu 
    50                  55                  60                  
Ile Val Val Cys Val Thr Asp His Arg Ala Val Arg Glu Leu Leu Glu 
65                  70                  75                  80  
Pro Leu Gly Asp Ala Leu Pro Gly Arg Val Leu Val Asn Leu Thr Ser 
                85                  90                  95      
Gly Thr Ser Arg Glu Ala Arg Glu Asn Ala Glu His Thr Ala Ala Gln 
            100                 105                 110         
Gly Ala Thr Tyr Leu Asp Gly Val Ile Leu Gly Ile Pro Ser Gly Ile 
        115                 120                 125             
Gly Thr Pro Glu Ala Thr Ile Leu Tyr Ser Gly Pro Arg Ser Thr Phe 
    130                 135                 140                 
Asp Glu His Glu Ser Ala Leu Arg Ala Leu Gly Pro Gly Ala Thr Phe 
145                 150                 155                 160 
Val Gly Glu Asp His Gly Leu Ala Asn Leu Val Asp Ile Ala Met Leu 
                165                 170                 175     
Ser Val Met Trp Gly Leu Leu Asn Gly Phe Leu His Gly Thr Ala Leu 
            180                 185                 190         
Leu Gly Thr Ala Gly Val Ser Ala Thr Ser Val Val Pro Met Leu Gly 
        195                 200                 205             
Gln Gly Ile Cys Val Val Thr Gly Trp Ile Ala Arg Tyr Ala Asp Gln 
    210                 215                 220                 
Ile Asp Ala Gly Val Tyr Pro Ala Asp Asp Lys Thr Ile Asp Thr Gln 
225                 230                 235                 240 
Arg Ala Ala Met Gln His Leu Val Glu Glu Ser Arg Ala Ala Gly Ile 
                245                 250                 255     
Asn Gly Glu Leu Pro Ala Phe Val Thr Lys Leu Ala Asp Arg Ala Val 
            260                 265                 270         
Ala Glu Gly Arg Gly Gly Asp Ser Tyr Ala Ala Leu Ile Glu Gln Phe 
        275                 280                 285             
Arg Lys Ser Ala                                                 
    290                                                         

 
<210> 145
<211> 879
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Imine reductase variant from Pseudonocardia spinosispora
 
<400> 145
atgagtgatt cacctgtgcc cgtgacagtt ctgggtcttg gtctgatggg tcaggcctta 60
gcgggagcgt ttctggcagc cggtcaccct acgacggttt ggaatcgcag cgcagctaaa 120
gctgatgaac tggttggtcg cggtgcaacc ctggccgcgt ctcccgcggc tgccgtcgca 180
gcgggtagtc tgatcgttgt ttgcgtcaca gatcatcgtg cggtgcgcga actgctggaa 240
ccacttggcg atgcactgcc aggtcgcgtt ctggtcaacc tgacgagcgg tacgtcgcgt 300
gaagcccgtg agaacgcaga acatactgcg gctcagggag ccacctacct ggatggtgtg 360
attctgacgg ccccaagcgg cattggcacc ccagaagcta ccattctgta ttcgggaccg 420
cgcagtacct tcgatgaaca tgaaagcgca ctgcgcgcat taggtccggg ggcgaccttt 480
gtaggggaag atcacggact ggctaatctg tacgatattg cacacctgag cgtcatgtgg 540
ggcctgctga atggtttcct gcatggaacc gcactgctgg gaaccgcggg cgtttcggca 600
acctctgtgg ttccgatgct gggtcaaggt atcggagtgg tgaccggttg gattgcgcgc 660
tacgcagatc agattgatgc cggtgtctat ccagcagacg acgcgacgat tgatacccag 720
cgtgctgcaa tgcagcacct ggtcgaggag agccgtgcgg cgggcatcaa tggcgaactt 780
ccggcgtttg tgaccaaact ggccgatcgc gcagtggcag aaggtcgtgg tggtgactct 840
tacgctgcac tgattgaaca gtttcgcaag tcagcttaa                        879

 
<210> 146
<211> 292
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Imine reductase variant from Pseudonocardia spinosispora
 
<400> 146
Met Ser Asp Ser Pro Val Pro Val Thr Val Leu Gly Leu Gly Leu Met 
1               5                   10                  15      
Gly Gln Ala Leu Ala Gly Ala Phe Leu Ala Ala Gly His Pro Thr Thr 
            20                  25                  30          
Val Trp Asn Arg Ser Ala Ala Lys Ala Asp Glu Leu Val Gly Arg Gly 
        35                  40                  45              
Ala Thr Leu Ala Ala Ser Pro Ala Ala Ala Val Ala Ala Gly Ser Leu 
    50                  55                  60                  
Ile Val Val Cys Val Thr Asp His Arg Ala Val Arg Glu Leu Leu Glu 
65                  70                  75                  80  
Pro Leu Gly Asp Ala Leu Pro Gly Arg Val Leu Val Asn Leu Thr Ser 
                85                  90                  95      
Gly Thr Ser Arg Glu Ala Arg Glu Asn Ala Glu His Thr Ala Ala Gln 
            100                 105                 110         
Gly Ala Thr Tyr Leu Asp Gly Val Ile Leu Thr Ala Pro Ser Gly Ile 
        115                 120                 125             
Gly Thr Pro Glu Ala Thr Ile Leu Tyr Ser Gly Pro Arg Ser Thr Phe 
    130                 135                 140                 
Asp Glu His Glu Ser Ala Leu Arg Ala Leu Gly Pro Gly Ala Thr Phe 
145                 150                 155                 160 
Val Gly Glu Asp His Gly Leu Ala Asn Leu Tyr Asp Ile Ala His Leu 
                165                 170                 175     
Ser Val Met Trp Gly Leu Leu Asn Gly Phe Leu His Gly Thr Ala Leu 
            180                 185                 190         
Leu Gly Thr Ala Gly Val Ser Ala Thr Ser Val Val Pro Met Leu Gly 
        195                 200                 205             
Gln Gly Ile Gly Val Val Thr Gly Trp Ile Ala Arg Tyr Ala Asp Gln 
    210                 215                 220                 
Ile Asp Ala Gly Val Tyr Pro Ala Asp Asp Ala Thr Ile Asp Thr Gln 
225                 230                 235                 240 
Arg Ala Ala Met Gln His Leu Val Glu Glu Ser Arg Ala Ala Gly Ile 
                245                 250                 255     
Asn Gly Glu Leu Pro Ala Phe Val Thr Lys Leu Ala Asp Arg Ala Val 
            260                 265                 270         
Ala Glu Gly Arg Gly Gly Asp Ser Tyr Ala Ala Leu Ile Glu Gln Phe 
        275                 280                 285             
Arg Lys Ser Ala                                                 
    290                                                         

 
<210> 147
<211> 879
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Imine reductase variant from Pseudonocardia spinosispora
 
<400> 147
atgagtgatt cacctgtgcc cgtgacagtt ctgggtcttg gtctgatggg tcaggcctta 60
gcgggagcgt ttctggcagc cggtcaccct acgacggttt ggaatcgcag cgcagctaaa 120
gctgatgaac tggttggtcg cggtgcaacc ctggccgcgt ctcccgcggc tgccgtcgca 180
gcgggtagtc tgatcgttgt ttgcgtcaca gatcatcgtg cggtgcgcga actgctggaa 240
ccacttggcg atgcactgcc aggtcgcgtt ctggtcaacc tgacgagcgg tacgtcgcgt 300
gaagcccgtg agaacgcaga acatactgcg gctcagggag ccacctacct ggatggtgtg 360
attctgacga ttccaagcgg cattggcacc ccagaagcta ccattctgta ttcgggaccg 420
cgcagtacct tcgatgaaca tgaaagcgca ctgcgcgcat taggtccggg ggcgaccttt 480
gtaggggaag atcacggact ggctaatctg tacgatgccg cacacctgag cgtcatgtgg 540
ggcctgctga atggtttcct gcatggaacc gcactgctgg gaaccgcggg cgtttcggca 600
acctctgtgg ttccgatgct gggtcaaggt atcggagtgg tgaccggttg gattgcgcgc 660
tacgcagatc agattgatgc cggtgtctat ccagcagacg acaagacgat tgatacccag 720
cgtgctgcaa tgcagcacct ggtcgaggag agccgtgcgg cgggcatcaa tggcgaactt 780
ccggcgtttg tgaccaaact ggccgatcgc gcagtggcag aaggtcgtgg tggtgactct 840
tacgctgcac tgattgaaca gtttcgcaag tcagcttaa                        879

 
<210> 148
<211> 292
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Imine reductase variant from Pseudonocardia spinosispora
 
<400> 148
Met Ser Asp Ser Pro Val Pro Val Thr Val Leu Gly Leu Gly Leu Met 
1               5                   10                  15      
Gly Gln Ala Leu Ala Gly Ala Phe Leu Ala Ala Gly His Pro Thr Thr 
            20                  25                  30          
Val Trp Asn Arg Ser Ala Ala Lys Ala Asp Glu Leu Val Gly Arg Gly 
        35                  40                  45              
Ala Thr Leu Ala Ala Ser Pro Ala Ala Ala Val Ala Ala Gly Ser Leu 
    50                  55                  60                  
Ile Val Val Cys Val Thr Asp His Arg Ala Val Arg Glu Leu Leu Glu 
65                  70                  75                  80  
Pro Leu Gly Asp Ala Leu Pro Gly Arg Val Leu Val Asn Leu Thr Ser 
                85                  90                  95      
Gly Thr Ser Arg Glu Ala Arg Glu Asn Ala Glu His Thr Ala Ala Gln 
            100                 105                 110         
Gly Ala Thr Tyr Leu Asp Gly Val Ile Leu Thr Ile Pro Ser Gly Ile 
        115                 120                 125             
Gly Thr Pro Glu Ala Thr Ile Leu Tyr Ser Gly Pro Arg Ser Thr Phe 
    130                 135                 140                 
Asp Glu His Glu Ser Ala Leu Arg Ala Leu Gly Pro Gly Ala Thr Phe 
145                 150                 155                 160 
Val Gly Glu Asp His Gly Leu Ala Asn Leu Tyr Asp Ala Ala His Leu 
                165                 170                 175     
Ser Val Met Trp Gly Leu Leu Asn Gly Phe Leu His Gly Thr Ala Leu 
            180                 185                 190         
Leu Gly Thr Ala Gly Val Ser Ala Thr Ser Val Val Pro Met Leu Gly 
        195                 200                 205             
Gln Gly Ile Gly Val Val Thr Gly Trp Ile Ala Arg Tyr Ala Asp Gln 
    210                 215                 220                 
Ile Asp Ala Gly Val Tyr Pro Ala Asp Asp Lys Thr Ile Asp Thr Gln 
225                 230                 235                 240 
Arg Ala Ala Met Gln His Leu Val Glu Glu Ser Arg Ala Ala Gly Ile 
                245                 250                 255     
Asn Gly Glu Leu Pro Ala Phe Val Thr Lys Leu Ala Asp Arg Ala Val 
            260                 265                 270         
Ala Glu Gly Arg Gly Gly Asp Ser Tyr Ala Ala Leu Ile Glu Gln Phe 
        275                 280                 285             
Arg Lys Ser Ala                                                 
    290                                                         

 
<210> 149
<211> 879
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Imine reductase variant from Pseudonocardia spinosispora
 
<400> 149
atgagtgatt cacctgtgcc cgtgacagtt ctgggtcttg gtctgatggg tcaggcctta 60
gcgggagcgt ttctggcagc cggtcaccct acgacggttt ggaatcgcag cgcagctaaa 120
gctgatgaac tggttggtcg cggtgcaacc ctggccgcgt ctcccgcggc tgccgtcgca 180
gcgggtagtc tgatcgttgt ttgcgtcaca gatcatcgtg cggtgcgcga actgctggaa 240
ccacttggcg atgcactgcc aggtcgcgtt ctggtcaacc tgacgagcgg tacgtcgcgt 300
gaagcccgtg agaacgcaga acatactgcg gctcagggag ccacctacct ggatggtgtg 360
attctgggta ttccaagcgg cattggcacc ccagaagcta ccattctgta ttcgggaccg 420
cgcagtacct tcgatgaaca tgaaagcgca ctgcgcgcat taggtccggg ggcgaccttt 480
gtaggggaag atcacggact ggctaatctg tacgatgccg caggcctgag cgtcatgtgg 540
ggcctgctga atggtttcct gcatggaacc gcactgctgg gaaccgcggg cgtttcggca 600
acctctgtgg ttccgatgct gggtcaaggt atcgaagctg tgaccggttg gattgcgcgc 660
tacgcagatc agattgatgc cggtgtctat ccagcagacg acgccacgat tgatacccag 720
cgtgctgcaa tgcagcacct ggtcgaggag agccgtgcgg cgggcatcaa tggcgaactt 780
ccggcgtttg tgaccaaact ggccgatcgc gcagtggcag aaggtcgtgg tggtgactct 840
tacgctgcac tgattgaaca gtttcgcaag tcagcttaa                        879

 
<210> 150
<211> 292
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Imine reductase variant from Pseudonocardia spinosispora
 
<400> 150
Met Ser Asp Ser Pro Val Pro Val Thr Val Leu Gly Leu Gly Leu Met 
1               5                   10                  15      
Gly Gln Ala Leu Ala Gly Ala Phe Leu Ala Ala Gly His Pro Thr Thr 
            20                  25                  30          
Val Trp Asn Arg Ser Ala Ala Lys Ala Asp Glu Leu Val Gly Arg Gly 
        35                  40                  45              
Ala Thr Leu Ala Ala Ser Pro Ala Ala Ala Val Ala Ala Gly Ser Leu 
    50                  55                  60                  
Ile Val Val Cys Val Thr Asp His Arg Ala Val Arg Glu Leu Leu Glu 
65                  70                  75                  80  
Pro Leu Gly Asp Ala Leu Pro Gly Arg Val Leu Val Asn Leu Thr Ser 
                85                  90                  95      
Gly Thr Ser Arg Glu Ala Arg Glu Asn Ala Glu His Thr Ala Ala Gln 
            100                 105                 110         
Gly Ala Thr Tyr Leu Asp Gly Val Ile Leu Gly Ile Pro Ser Gly Ile 
        115                 120                 125             
Gly Thr Pro Glu Ala Thr Ile Leu Tyr Ser Gly Pro Arg Ser Thr Phe 
    130                 135                 140                 
Asp Glu His Glu Ser Ala Leu Arg Ala Leu Gly Pro Gly Ala Thr Phe 
145                 150                 155                 160 
Val Gly Glu Asp His Gly Leu Ala Asn Leu Tyr Asp Ala Ala Gly Leu 
                165                 170                 175     
Ser Val Met Trp Gly Leu Leu Asn Gly Phe Leu His Gly Thr Ala Leu 
            180                 185                 190         
Leu Gly Thr Ala Gly Val Ser Ala Thr Ser Val Val Pro Met Leu Gly 
        195                 200                 205             
Gln Gly Ile Glu Ala Val Thr Gly Trp Ile Ala Arg Tyr Ala Asp Gln 
    210                 215                 220                 
Ile Asp Ala Gly Val Tyr Pro Ala Asp Asp Ala Thr Ile Asp Thr Gln 
225                 230                 235                 240 
Arg Ala Ala Met Gln His Leu Val Glu Glu Ser Arg Ala Ala Gly Ile 
                245                 250                 255     
Asn Gly Glu Leu Pro Ala Phe Val Thr Lys Leu Ala Asp Arg Ala Val 
            260                 265                 270         
Ala Glu Gly Arg Gly Gly Asp Ser Tyr Ala Ala Leu Ile Glu Gln Phe 
        275                 280                 285             
Arg Lys Ser Ala                                                 
    290                                                         

 
<210> 151
<211> 879
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Imine reductase variant from Pseudonocardia spinosispora
 
<400> 151
atgagtgatt cacctgtgcc cgtgacagtt ctgggtcttg gtctgatggg tcaggcctta 60
gcgggagcgt ttctggcagc cggtcaccct acgacggttt ggaatcgcag cgcagctaaa 120
gctgatgaac tggttggtcg cggtgcaacc ctggccgcgt ctcccgcggc tgccgtcgca 180
gcgggtagtc tgatcgttgt ttgcgtcaca gatcatcgtg cggtgcgcga actgctggaa 240
ccacttggcg atgcactgcc aggtcgcgtt ctggtcaacc tgacgagcgg tacgtcgcgt 300
gaagcccgtg agaacgcaga acatactgcg gctcagggag ccacctacct ggatggtgtg 360
attctgggta ttccaagcgg cattggcacc ccagaagcta ccattctgta ttcgggaccg 420
cgcagtacct tcgatgaaca tgaaagcgca ctgcgcgcat taggtccggg ggcgaccttt 480
gtaggggaag atcacggact ggctaatctg tacgatgccg caggcctgag cgtcatgtgg 540
ggcctgctga atggtttcct gcatggaacc gcactgctgg gaaccgcggg cgtttcggca 600
acctctgtgg ttccgatgct gggtcaaggt atctgcgctg tgaccggttg gattgcgcgc 660
tacgcagatc agattgatgc cggtgtctat ccagcagacg acaagacgat tgatacccag 720
cgtgctgcaa tgcagcacct ggtcgaggag agccgtgcgg cgggcatcaa tggcgaactt 780
ccggcgtttg tgaccaaact ggccgatcgc gcagtggcag aaggtcgtgg tggtgactct 840
tacgctgcac tgattgaaca gtttcgcaag tcagcttaa                        879

 
<210> 152
<211> 292
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Imine reductase variant from Pseudonocardia spinosispora
 
<400> 152
Met Ser Asp Ser Pro Val Pro Val Thr Val Leu Gly Leu Gly Leu Met 
1               5                   10                  15      
Gly Gln Ala Leu Ala Gly Ala Phe Leu Ala Ala Gly His Pro Thr Thr 
            20                  25                  30          
Val Trp Asn Arg Ser Ala Ala Lys Ala Asp Glu Leu Val Gly Arg Gly 
        35                  40                  45              
Ala Thr Leu Ala Ala Ser Pro Ala Ala Ala Val Ala Ala Gly Ser Leu 
    50                  55                  60                  
Ile Val Val Cys Val Thr Asp His Arg Ala Val Arg Glu Leu Leu Glu 
65                  70                  75                  80  
Pro Leu Gly Asp Ala Leu Pro Gly Arg Val Leu Val Asn Leu Thr Ser 
                85                  90                  95      
Gly Thr Ser Arg Glu Ala Arg Glu Asn Ala Glu His Thr Ala Ala Gln 
            100                 105                 110         
Gly Ala Thr Tyr Leu Asp Gly Val Ile Leu Gly Ile Pro Ser Gly Ile 
        115                 120                 125             
Gly Thr Pro Glu Ala Thr Ile Leu Tyr Ser Gly Pro Arg Ser Thr Phe 
    130                 135                 140                 
Asp Glu His Glu Ser Ala Leu Arg Ala Leu Gly Pro Gly Ala Thr Phe 
145                 150                 155                 160 
Val Gly Glu Asp His Gly Leu Ala Asn Leu Tyr Asp Ala Ala Gly Leu 
                165                 170                 175     
Ser Val Met Trp Gly Leu Leu Asn Gly Phe Leu His Gly Thr Ala Leu 
            180                 185                 190         
Leu Gly Thr Ala Gly Val Ser Ala Thr Ser Val Val Pro Met Leu Gly 
        195                 200                 205             
Gln Gly Ile Cys Ala Val Thr Gly Trp Ile Ala Arg Tyr Ala Asp Gln 
    210                 215                 220                 
Ile Asp Ala Gly Val Tyr Pro Ala Asp Asp Lys Thr Ile Asp Thr Gln 
225                 230                 235                 240 
Arg Ala Ala Met Gln His Leu Val Glu Glu Ser Arg Ala Ala Gly Ile 
                245                 250                 255     
Asn Gly Glu Leu Pro Ala Phe Val Thr Lys Leu Ala Asp Arg Ala Val 
            260                 265                 270         
Ala Glu Gly Arg Gly Gly Asp Ser Tyr Ala Ala Leu Ile Glu Gln Phe 
        275                 280                 285             
Arg Lys Ser Ala                                                 
    290                                                         

 
<210> 153
<211> 879
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Imine reductase variant from Pseudonocardia spinosispora
 
<400> 153
atgagtgatt cacctgtgcc cgtgacagtt ctgggtcttg gtctgatggg tcaggcctta 60
gcgggagcgt ttctggcagc cggtcaccct acgacggttt ggaatcgcag cgcagctaaa 120
gctgatgaac tggttggtcg cggtgcaacc ctggccgcgt ctcccgcggc tgccgtcgca 180
gcgggtagtc tgatcgttgt ttgcgtcaca gatcatcgtg cggtgcgcga actgctggaa 240
ccacttggcg atgcactgcc aggtcgcgtt ctggtcaacc tgacgagcgg tacgtcgcgt 300
gaagcccgtg agaacgcaga acatactgcg gctcagggag ccacctacct ggatggtgtg 360
attctgacga ttccaagcgg cattggcacc ccagaagcta ccattctgta ttcgggaccg 420
cgcagtacct tcgatgaaca tgaaagcgca ctgcgcgcat taggtccggg ggcgaccttt 480
gtaggggaag atcacggact ggctaatctg tacgatgccg caatgctgag cgtcatgtgg 540
ggcctgctga atggtttcct gcatggaacc gcactgctgg gaaccgcggg cgtttcggca 600
acctctgtgg ttccgatgct gggtcaaggt atcgtagtgg tgaccggttg gattgcgcgc 660
tacgcagatc agattgatgc cggtgtctat ccagcagacg acaagacgat tgatacccag 720
cgtgctgcaa tgcagcacct ggtcgaggag agccgtgcgg cgggcatcaa tggcgaactt 780
ccggcgtttg tgaccaaact ggccgatcgc gcagtggcag aaggtcgtgg tggtgactct 840
tacgctgcac tgattgaaca gtttcgcaag tcagcttaa                        879

 
<210> 154
<211> 292
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Imine reductase variant from Pseudonocardia spinosispora
 
<400> 154
Met Ser Asp Ser Pro Val Pro Val Thr Val Leu Gly Leu Gly Leu Met 
1               5                   10                  15      
Gly Gln Ala Leu Ala Gly Ala Phe Leu Ala Ala Gly His Pro Thr Thr 
            20                  25                  30          
Val Trp Asn Arg Ser Ala Ala Lys Ala Asp Glu Leu Val Gly Arg Gly 
        35                  40                  45              
Ala Thr Leu Ala Ala Ser Pro Ala Ala Ala Val Ala Ala Gly Ser Leu 
    50                  55                  60                  
Ile Val Val Cys Val Thr Asp His Arg Ala Val Arg Glu Leu Leu Glu 
65                  70                  75                  80  
Pro Leu Gly Asp Ala Leu Pro Gly Arg Val Leu Val Asn Leu Thr Ser 
                85                  90                  95      
Gly Thr Ser Arg Glu Ala Arg Glu Asn Ala Glu His Thr Ala Ala Gln 
            100                 105                 110         
Gly Ala Thr Tyr Leu Asp Gly Val Ile Leu Thr Ile Pro Ser Gly Ile 
        115                 120                 125             
Gly Thr Pro Glu Ala Thr Ile Leu Tyr Ser Gly Pro Arg Ser Thr Phe 
    130                 135                 140                 
Asp Glu His Glu Ser Ala Leu Arg Ala Leu Gly Pro Gly Ala Thr Phe 
145                 150                 155                 160 
Val Gly Glu Asp His Gly Leu Ala Asn Leu Tyr Asp Ala Ala Met Leu 
                165                 170                 175     
Ser Val Met Trp Gly Leu Leu Asn Gly Phe Leu His Gly Thr Ala Leu 
            180                 185                 190         
Leu Gly Thr Ala Gly Val Ser Ala Thr Ser Val Val Pro Met Leu Gly 
        195                 200                 205             
Gln Gly Ile Val Val Val Thr Gly Trp Ile Ala Arg Tyr Ala Asp Gln 
    210                 215                 220                 
Ile Asp Ala Gly Val Tyr Pro Ala Asp Asp Lys Thr Ile Asp Thr Gln 
225                 230                 235                 240 
Arg Ala Ala Met Gln His Leu Val Glu Glu Ser Arg Ala Ala Gly Ile 
                245                 250                 255     
Asn Gly Glu Leu Pro Ala Phe Val Thr Lys Leu Ala Asp Arg Ala Val 
            260                 265                 270         
Ala Glu Gly Arg Gly Gly Asp Ser Tyr Ala Ala Leu Ile Glu Gln Phe 
        275                 280                 285             
Arg Lys Ser Ala                                                 
    290                                                         

 
<210> 155
<211> 879
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Imine reductase variant from Pseudonocardia spinosispora
 
<400> 155
atgagtgatt cacctgtgcc cgtgacagtt ctgggtcttg gtctgatggg tcaggcctta 60
gcgggagcgt ttctggcagc cggtcaccct acgacggttt ggaatcgcag cgcagctaaa 120
gctgatgaac tggttggtcg cggtgcaacc ctggccgcgt ctcccgcggc tgccgtcgca 180
gcgggtagtc tgatcgttgt ttgcgtcaca gatcatcgtg cggtgcgcga actgctggaa 240
ccacttggcg atgcactgcc aggtcgcgtt ctggtcaacc tgacgagcgg tacgtcgcgt 300
gaagcccgtg agaacgcaga acatactgcg gctcagggag ccacctacct ggatggtgtg 360
attctgacga ttccaagcgg cattggcacc ccagaagcta ccattctgta ttcgggaccg 420
cgcagtacct tcgatgaaca tgaaagcgca ctgcgcgcat taggtccggg ggcgaccttt 480
gtaggggaag atcacggact ggctaatctg tacgatgccg caggcctgag cgtcatgtgg 540
ggcctgctga atggtttcct gcatggaacc gcactgctgg gaaccgcggg cgtttcggca 600
acctctgtgg ttccgatgct gggtcaaggt atctgcgtgg tgaccggttg gattgcgcgc 660
tacgcagatc agattgatgc cggtgtctat ccagcagacg acaagacgat tgatacccag 720
cgtgctgcaa tgcagcacct ggtcgaggag agccgtgcgg cgggcatcaa tggcgaactt 780
ccggcgtttg tgaccaaact ggccgatcgc gcagtggcag aaggtcgtgg tggtgactct 840
tacgctgcac tgattgaaca gtttcgcaag tcagcttaa                        879

 
<210> 156
<211> 292
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Imine reductase variant from Pseudonocardia spinosispora
 
<400> 156
Met Ser Asp Ser Pro Val Pro Val Thr Val Leu Gly Leu Gly Leu Met 
1               5                   10                  15      
Gly Gln Ala Leu Ala Gly Ala Phe Leu Ala Ala Gly His Pro Thr Thr 
            20                  25                  30          
Val Trp Asn Arg Ser Ala Ala Lys Ala Asp Glu Leu Val Gly Arg Gly 
        35                  40                  45              
Ala Thr Leu Ala Ala Ser Pro Ala Ala Ala Val Ala Ala Gly Ser Leu 
    50                  55                  60                  
Ile Val Val Cys Val Thr Asp His Arg Ala Val Arg Glu Leu Leu Glu 
65                  70                  75                  80  
Pro Leu Gly Asp Ala Leu Pro Gly Arg Val Leu Val Asn Leu Thr Ser 
                85                  90                  95      
Gly Thr Ser Arg Glu Ala Arg Glu Asn Ala Glu His Thr Ala Ala Gln 
            100                 105                 110         
Gly Ala Thr Tyr Leu Asp Gly Val Ile Leu Thr Ile Pro Ser Gly Ile 
        115                 120                 125             
Gly Thr Pro Glu Ala Thr Ile Leu Tyr Ser Gly Pro Arg Ser Thr Phe 
    130                 135                 140                 
Asp Glu His Glu Ser Ala Leu Arg Ala Leu Gly Pro Gly Ala Thr Phe 
145                 150                 155                 160 
Val Gly Glu Asp His Gly Leu Ala Asn Leu Tyr Asp Ala Ala Gly Leu 
                165                 170                 175     
Ser Val Met Trp Gly Leu Leu Asn Gly Phe Leu His Gly Thr Ala Leu 
            180                 185                 190         
Leu Gly Thr Ala Gly Val Ser Ala Thr Ser Val Val Pro Met Leu Gly 
        195                 200                 205             
Gln Gly Ile Cys Val Val Thr Gly Trp Ile Ala Arg Tyr Ala Asp Gln 
    210                 215                 220                 
Ile Asp Ala Gly Val Tyr Pro Ala Asp Asp Lys Thr Ile Asp Thr Gln 
225                 230                 235                 240 
Arg Ala Ala Met Gln His Leu Val Glu Glu Ser Arg Ala Ala Gly Ile 
                245                 250                 255     
Asn Gly Glu Leu Pro Ala Phe Val Thr Lys Leu Ala Asp Arg Ala Val 
            260                 265                 270         
Ala Glu Gly Arg Gly Gly Asp Ser Tyr Ala Ala Leu Ile Glu Gln Phe 
        275                 280                 285             
Arg Lys Ser Ala                                                 
    290                                                         

 
<210> 157
<211> 879
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Imine reductase variant from Pseudonocardia spinosispora
 
<400> 157
atgagtgatt cacctgtgcc cgtgacagtt ctgggtcttg gtctgatggg tcaggcctta 60
gcgggagcgt ttctggcagc cggtcaccct acgacggttt ggaatcgcag cgcagctaaa 120
gctgatgaac tggttggtcg cggtgcaacc ctggccgcgt ctcccgcggc tgccgtcgca 180
gcgggtagtc tgatcgttgt ttgcgtcaca gatcatcgtg cggtgcgcga actgctggaa 240
ccacttggcg atgcactgcc aggtcgcgtt ctggtcaacc tgacgagcgg tacgtcgcgt 300
gaagcccgtg agaacgcaga acatactgcg gctcagggag ccacctacct ggatggtgtg 360
attctgacga ttccaagcgg cattggcacc ccagaagcta ccattctgta ttcgggaccg 420
cgcagtacct tcgatgaaca tgaaagcgca ctgcgcgcat taggtccggg ggcgaccttt 480
gtaggggaag atcacggact ggctaatctg gttgatattg caatgctgag cgtcatgtgg 540
ggcctgctga atggtttcct gcatggaacc gcactgctgg gaaccgcggg cgtttcggca 600
acctctgtgg ttccgatgct gggtcaaggt atcgaagtgg tgaccggttg gattgcgcgc 660
tacgcagatc agattgatgc cggtgtctat ccagcagacg acaagacgat tgatacccag 720
cgtgctgcaa tgcagcacct ggtcgaggag agccgtgcgg cgggcatcaa tggcgaactt 780
ccggcgtttg tgaccaaact ggccgatcgc gcagtggcag aaggtcgtgg tggtgactct 840
tacgctgcac tgattgaaca gtttcgcaag tcagcttaa                        879

 
<210> 158
<211> 292
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Imine reductase variant from Pseudonocardia spinosispora
 
<400> 158
Met Ser Asp Ser Pro Val Pro Val Thr Val Leu Gly Leu Gly Leu Met 
1               5                   10                  15      
Gly Gln Ala Leu Ala Gly Ala Phe Leu Ala Ala Gly His Pro Thr Thr 
            20                  25                  30          
Val Trp Asn Arg Ser Ala Ala Lys Ala Asp Glu Leu Val Gly Arg Gly 
        35                  40                  45              
Ala Thr Leu Ala Ala Ser Pro Ala Ala Ala Val Ala Ala Gly Ser Leu 
    50                  55                  60                  
Ile Val Val Cys Val Thr Asp His Arg Ala Val Arg Glu Leu Leu Glu 
65                  70                  75                  80  
Pro Leu Gly Asp Ala Leu Pro Gly Arg Val Leu Val Asn Leu Thr Ser 
                85                  90                  95      
Gly Thr Ser Arg Glu Ala Arg Glu Asn Ala Glu His Thr Ala Ala Gln 
            100                 105                 110         
Gly Ala Thr Tyr Leu Asp Gly Val Ile Leu Thr Ile Pro Ser Gly Ile 
        115                 120                 125             
Gly Thr Pro Glu Ala Thr Ile Leu Tyr Ser Gly Pro Arg Ser Thr Phe 
    130                 135                 140                 
Asp Glu His Glu Ser Ala Leu Arg Ala Leu Gly Pro Gly Ala Thr Phe 
145                 150                 155                 160 
Val Gly Glu Asp His Gly Leu Ala Asn Leu Val Asp Ile Ala Met Leu 
                165                 170                 175     
Ser Val Met Trp Gly Leu Leu Asn Gly Phe Leu His Gly Thr Ala Leu 
            180                 185                 190         
Leu Gly Thr Ala Gly Val Ser Ala Thr Ser Val Val Pro Met Leu Gly 
        195                 200                 205             
Gln Gly Ile Glu Val Val Thr Gly Trp Ile Ala Arg Tyr Ala Asp Gln 
    210                 215                 220                 
Ile Asp Ala Gly Val Tyr Pro Ala Asp Asp Lys Thr Ile Asp Thr Gln 
225                 230                 235                 240 
Arg Ala Ala Met Gln His Leu Val Glu Glu Ser Arg Ala Ala Gly Ile 
                245                 250                 255     
Asn Gly Glu Leu Pro Ala Phe Val Thr Lys Leu Ala Asp Arg Ala Val 
            260                 265                 270         
Ala Glu Gly Arg Gly Gly Asp Ser Tyr Ala Ala Leu Ile Glu Gln Phe 
        275                 280                 285             
Arg Lys Ser Ala                                                 
    290                                                         

 
<210> 159
<211> 879
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Imine reductase variant from Pseudonocardia spinosispora
 
<400> 159
atgagtgatt cacctgtgcc cgtgacagtt ctgggtcttg gtctgatggg tcaggcctta 60
gcgggagcgt ttctggcagc cggtcaccct acgacggttt ggaatcgcag cgcagctaaa 120
gctgatgaac tggttggtcg cggtgcaacc ctggccgcgt ctcccgcggc tgccgtcgca 180
gcgggtagtc tgatcgttgt ttgcgtcaca gatcatcgtg cggtgcgcga actgctggaa 240
ccacttggcg atgcactgcc aggtcgcgtt ctggtcaacc tgacgagcgg tacgtcgcgt 300
gaagcccgtg agaacgcaga acatactgcg gctcagggag ccacctacct ggatggtgtg 360
attctgacgg gcccaagcgg cattggcacc ccagaagcta ccattctgta ttcgggaccg 420
cgcagtacct tcgatgaaca tgaaagcgca ctgcgcgcat taggtccggg ggcgaccttt 480
gtaggggaag atcacggact ggctaatctg tacgatgccg caatgctgag cgtcatgtgg 540
ggcctgctga atggtttcct gcatggaacc gcactgctgg gaaccgcggg cgtttcggca 600
acctctgtgg ttccgatgct gggtcaaggt atcgaagtgg tgaccggttg gattgcgcgc 660
tacgcagatc agattgatgc cggtgtctat ccagcagacg acaagacgat tgatacccag 720
cgtgctgcaa tgcagcacct ggtcgaggag agccgtgcgg cgggcatcaa tggcgaactt 780
ccggcgtttg tgaccaaact ggccgatcgc gcagtggcag aaggtcgtgg tggtgactct 840
tacgctgcac tgattgaaca gtttcgcaag tcagcttaa                        879

 
<210> 160
<211> 292
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Imine reductase variant from Pseudonocardia spinosispora
 
<400> 160
Met Ser Asp Ser Pro Val Pro Val Thr Val Leu Gly Leu Gly Leu Met 
1               5                   10                  15      
Gly Gln Ala Leu Ala Gly Ala Phe Leu Ala Ala Gly His Pro Thr Thr 
            20                  25                  30          
Val Trp Asn Arg Ser Ala Ala Lys Ala Asp Glu Leu Val Gly Arg Gly 
        35                  40                  45              
Ala Thr Leu Ala Ala Ser Pro Ala Ala Ala Val Ala Ala Gly Ser Leu 
    50                  55                  60                  
Ile Val Val Cys Val Thr Asp His Arg Ala Val Arg Glu Leu Leu Glu 
65                  70                  75                  80  
Pro Leu Gly Asp Ala Leu Pro Gly Arg Val Leu Val Asn Leu Thr Ser 
                85                  90                  95      
Gly Thr Ser Arg Glu Ala Arg Glu Asn Ala Glu His Thr Ala Ala Gln 
            100                 105                 110         
Gly Ala Thr Tyr Leu Asp Gly Val Ile Leu Thr Gly Pro Ser Gly Ile 
        115                 120                 125             
Gly Thr Pro Glu Ala Thr Ile Leu Tyr Ser Gly Pro Arg Ser Thr Phe 
    130                 135                 140                 
Asp Glu His Glu Ser Ala Leu Arg Ala Leu Gly Pro Gly Ala Thr Phe 
145                 150                 155                 160 
Val Gly Glu Asp His Gly Leu Ala Asn Leu Tyr Asp Ala Ala Met Leu 
                165                 170                 175     
Ser Val Met Trp Gly Leu Leu Asn Gly Phe Leu His Gly Thr Ala Leu 
            180                 185                 190         
Leu Gly Thr Ala Gly Val Ser Ala Thr Ser Val Val Pro Met Leu Gly 
        195                 200                 205             
Gln Gly Ile Glu Val Val Thr Gly Trp Ile Ala Arg Tyr Ala Asp Gln 
    210                 215                 220                 
Ile Asp Ala Gly Val Tyr Pro Ala Asp Asp Lys Thr Ile Asp Thr Gln 
225                 230                 235                 240 
Arg Ala Ala Met Gln His Leu Val Glu Glu Ser Arg Ala Ala Gly Ile 
                245                 250                 255     
Asn Gly Glu Leu Pro Ala Phe Val Thr Lys Leu Ala Asp Arg Ala Val 
            260                 265                 270         
Ala Glu Gly Arg Gly Gly Asp Ser Tyr Ala Ala Leu Ile Glu Gln Phe 
        275                 280                 285             
Arg Lys Ser Ala                                                 
    290                                                         

 
<210> 161
<211> 879
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Imine reductase variant from Pseudonocardia spinosispora
 
<400> 161
atgagtgatt cacctgtgcc cgtgacagtt ctgggtcttg gtctgatggg tcaggcctta 60
gcgggagcgt ttctggcagc cggtcaccct acgacggttt ggaatcgcag cgcagctaaa 120
gctgatgaac tggttggtcg cggtgcaacc ctggccgcgt ctcccgcggc tgccgtcgca 180
gcgggtagtc tgatcgttgt ttgcgtcaca gatcatcgtg cggtgcgcga actgctggaa 240
ccacttggcg atgcactgcc aggtcgcgtt ctggtcaacc tgacgagcgg tacgtcgcgt 300
gaagcccgtg agaacgcaga acatactgcg gctcagggag ccacctacct ggatggtgtg 360
attctgacgg gcccaagcgg cattggcacc ccagaagcta ccattctgta ttcgggaccg 420
cgcagtacct tcgatgaaca tgaaagcgca ctgcgcgcat taggtccggg ggcgaccttt 480
gtaggggaag atcacggact ggctgccctg tacgatgccg caggcctgag cgtcatgtgg 540
ggcctgctga atggtttcct gcatggaacc gcactgctgg gaaccgcggg cgtttcggca 600
acctctgtgg ttccgatgct gggtcaaggt atcgtagtgg tgaccggttg gattgcgcgc 660
tacgcagatc agattgatgc cggtgtctat ccagcagacg acaagacgat tgatacccag 720
cgtgctgcaa tgcagcacct ggtcgaggag agccgtgcgg cgggcatcaa tggcgaactt 780
ccggcgtttg tgaccaaact ggccgatcgc gcagtggcag aaggtcgtgg tggtgactct 840
tacgctgcac tgattgaaca gtttcgcaag tcagcttaa                        879

 
<210> 162
<211> 292
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Imine reductase variant from Pseudonocardia spinosispora
 
<400> 162
Met Ser Asp Ser Pro Val Pro Val Thr Val Leu Gly Leu Gly Leu Met 
1               5                   10                  15      
Gly Gln Ala Leu Ala Gly Ala Phe Leu Ala Ala Gly His Pro Thr Thr 
            20                  25                  30          
Val Trp Asn Arg Ser Ala Ala Lys Ala Asp Glu Leu Val Gly Arg Gly 
        35                  40                  45              
Ala Thr Leu Ala Ala Ser Pro Ala Ala Ala Val Ala Ala Gly Ser Leu 
    50                  55                  60                  
Ile Val Val Cys Val Thr Asp His Arg Ala Val Arg Glu Leu Leu Glu 
65                  70                  75                  80  
Pro Leu Gly Asp Ala Leu Pro Gly Arg Val Leu Val Asn Leu Thr Ser 
                85                  90                  95      
Gly Thr Ser Arg Glu Ala Arg Glu Asn Ala Glu His Thr Ala Ala Gln 
            100                 105                 110         
Gly Ala Thr Tyr Leu Asp Gly Val Ile Leu Thr Gly Pro Ser Gly Ile 
        115                 120                 125             
Gly Thr Pro Glu Ala Thr Ile Leu Tyr Ser Gly Pro Arg Ser Thr Phe 
    130                 135                 140                 
Asp Glu His Glu Ser Ala Leu Arg Ala Leu Gly Pro Gly Ala Thr Phe 
145                 150                 155                 160 
Val Gly Glu Asp His Gly Leu Ala Ala Leu Tyr Asp Ala Ala Gly Leu 
                165                 170                 175     
Ser Val Met Trp Gly Leu Leu Asn Gly Phe Leu His Gly Thr Ala Leu 
            180                 185                 190         
Leu Gly Thr Ala Gly Val Ser Ala Thr Ser Val Val Pro Met Leu Gly 
        195                 200                 205             
Gln Gly Ile Val Val Val Thr Gly Trp Ile Ala Arg Tyr Ala Asp Gln 
    210                 215                 220                 
Ile Asp Ala Gly Val Tyr Pro Ala Asp Asp Lys Thr Ile Asp Thr Gln 
225                 230                 235                 240 
Arg Ala Ala Met Gln His Leu Val Glu Glu Ser Arg Ala Ala Gly Ile 
                245                 250                 255     
Asn Gly Glu Leu Pro Ala Phe Val Thr Lys Leu Ala Asp Arg Ala Val 
            260                 265                 270         
Ala Glu Gly Arg Gly Gly Asp Ser Tyr Ala Ala Leu Ile Glu Gln Phe 
        275                 280                 285             
Arg Lys Ser Ala                                                 
    290                                                         

 
<210> 163
<211> 879
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Imine reductase variant from Pseudonocardia spinosispora
 
<400> 163
atgagtgatt cacctgtgcc cgtgacagtt ctgggtcttg gtctgatggg tcaggcctta 60
gcgggagcgt ttctggcagc cggtcaccct acgacggttt ggaatcgcag cgcagctaaa 120
gctgatgaac tggttggtcg cggtgcaacc ctggccgcgt ctcccgcggc tgccgtcgca 180
gcgggtagtc tgatcgttgt ttgcgtcaca gatcatcgtg cggtgcgcga actgctggaa 240
ccacttggcg atgcactgcc aggtcgcgtt ctggtcaacc tgacgagcgg tacgtcgcgt 300
gaagcccgtg agaacgcaga acatactgcg gctcagggag ccacctacct ggatggtgtg 360
attctgacga ttccaagcgg cattggcacc ccagaagcta ccattctgta ttcgggaccg 420
cgcagtacct tcgatgaaca tgaaagcgca ctgcgcgcat taggtccggg ggcgaccttt 480
gtaggggaag atcacggact ggctaatctg tacgatattg cacacctgag cgtcatgtgg 540
ggcctgctga atggtttcct gcatggaacc gcactgctgg gaaccgcggg cgtttcggca 600
acctctgtgg ttccgatgct gggtcaaggt atcgaagtgg tgaccggttg gattgcgcgc 660
tacgcagatc agattgatgc cggtgtctat ccagcagacg acaagacgat tgatacccag 720
cgtgctgcaa tgcagcacct ggtcgaggag agccgtgcgg cgggcatcaa tggcgaactt 780
ccggcgtttg tgaccaaact ggccgatcgc gcagtggcag aaggtcgtgg tggtgactct 840
tacgctgcac tgattgaaca gtttcgcaag tcagcttaa                        879

 
<210> 164
<211> 292
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Imine reductase variant from Pseudonocardia spinosispora
 
<400> 164
Met Ser Asp Ser Pro Val Pro Val Thr Val Leu Gly Leu Gly Leu Met 
1               5                   10                  15      
Gly Gln Ala Leu Ala Gly Ala Phe Leu Ala Ala Gly His Pro Thr Thr 
            20                  25                  30          
Val Trp Asn Arg Ser Ala Ala Lys Ala Asp Glu Leu Val Gly Arg Gly 
        35                  40                  45              
Ala Thr Leu Ala Ala Ser Pro Ala Ala Ala Val Ala Ala Gly Ser Leu 
    50                  55                  60                  
Ile Val Val Cys Val Thr Asp His Arg Ala Val Arg Glu Leu Leu Glu 
65                  70                  75                  80  
Pro Leu Gly Asp Ala Leu Pro Gly Arg Val Leu Val Asn Leu Thr Ser 
                85                  90                  95      
Gly Thr Ser Arg Glu Ala Arg Glu Asn Ala Glu His Thr Ala Ala Gln 
            100                 105                 110         
Gly Ala Thr Tyr Leu Asp Gly Val Ile Leu Thr Ile Pro Ser Gly Ile 
        115                 120                 125             
Gly Thr Pro Glu Ala Thr Ile Leu Tyr Ser Gly Pro Arg Ser Thr Phe 
    130                 135                 140                 
Asp Glu His Glu Ser Ala Leu Arg Ala Leu Gly Pro Gly Ala Thr Phe 
145                 150                 155                 160 
Val Gly Glu Asp His Gly Leu Ala Asn Leu Tyr Asp Ile Ala His Leu 
                165                 170                 175     
Ser Val Met Trp Gly Leu Leu Asn Gly Phe Leu His Gly Thr Ala Leu 
            180                 185                 190         
Leu Gly Thr Ala Gly Val Ser Ala Thr Ser Val Val Pro Met Leu Gly 
        195                 200                 205             
Gln Gly Ile Glu Val Val Thr Gly Trp Ile Ala Arg Tyr Ala Asp Gln 
    210                 215                 220                 
Ile Asp Ala Gly Val Tyr Pro Ala Asp Asp Lys Thr Ile Asp Thr Gln 
225                 230                 235                 240 
Arg Ala Ala Met Gln His Leu Val Glu Glu Ser Arg Ala Ala Gly Ile 
                245                 250                 255     
Asn Gly Glu Leu Pro Ala Phe Val Thr Lys Leu Ala Asp Arg Ala Val 
            260                 265                 270         
Ala Glu Gly Arg Gly Gly Asp Ser Tyr Ala Ala Leu Ile Glu Gln Phe 
        275                 280                 285             
Arg Lys Ser Ala                                                 
    290                                                         

 
<210> 165
<211> 879
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Imine reductase variant from Pseudonocardia spinosispora
 
<400> 165
atgagtgatt cacctgtgcc cgtgacagtt ctgggtcttg gtctgatggg tcaggcctta 60
gcgggagcgt ttctggcagc cggtcaccct acgacggttt ggaatcgcag cgcagctaaa 120
gctgatgaac tggttggtcg cggtgcaacc ctggccgcgt ctcccgcggc tgccgtcgca 180
gcgggtagtc tgatcgttgt ttgcgtcaca gatcatcgtg cggtgcgcga actgctggaa 240
ccacttggcg atgcactgcc aggtcgcgtt ctggtcaacc tgacgagcgg tacgtcgcgt 300
gaagcccgtg agaacgcaga acatactgcg gctcagggag ccacctacct ggatggtgtg 360
attctgacca ttccaagcgg cattggcacc ccagaagcta cgattctgta ttcgggaccg 420
cgcagtacct tcgatgaaca tgaaagcgca ctgcgcgcat taggtccggg ggcgaccttt 480
gtaggggaag atcacggact ggctaacctg tatgatgcag caatgtcgag cgtgatgtgg 540
ggcctgctga atggtttcct gcatggaacc gcactgctgg gaaccgcggg cgtttcggca 600
acctctgtgg ttccgatgct gggtcaaggt tgggaagcgg tgactggtct aattgcgcgc 660
tacgcagatc agattgatgc cggtgtctat ccagcagacg atgccacgat tgatacccag 720
cgtgctgcaa tgcagcacct ggtcgaggag agccgtgcgg cgggcatcaa tggcgaactt 780
ccggcgtttg tgaccaaact ggccgatcgc gcagtggcag aaggtcgtgg tggtgactct 840
tacgctgcac tgattgaaca gtttcgcaag tcagcttaa                        879

 
<210> 166
<211> 292
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Imine reductase variant from Pseudonocardia spinosispora
 
<400> 166
Met Ser Asp Ser Pro Val Pro Val Thr Val Leu Gly Leu Gly Leu Met 
1               5                   10                  15      
Gly Gln Ala Leu Ala Gly Ala Phe Leu Ala Ala Gly His Pro Thr Thr 
            20                  25                  30          
Val Trp Asn Arg Ser Ala Ala Lys Ala Asp Glu Leu Val Gly Arg Gly 
        35                  40                  45              
Ala Thr Leu Ala Ala Ser Pro Ala Ala Ala Val Ala Ala Gly Ser Leu 
    50                  55                  60                  
Ile Val Val Cys Val Thr Asp His Arg Ala Val Arg Glu Leu Leu Glu 
65                  70                  75                  80  
Pro Leu Gly Asp Ala Leu Pro Gly Arg Val Leu Val Asn Leu Thr Ser 
                85                  90                  95      
Gly Thr Ser Arg Glu Ala Arg Glu Asn Ala Glu His Thr Ala Ala Gln 
            100                 105                 110         
Gly Ala Thr Tyr Leu Asp Gly Val Ile Leu Thr Ile Pro Ser Gly Ile 
        115                 120                 125             
Gly Thr Pro Glu Ala Thr Ile Leu Tyr Ser Gly Pro Arg Ser Thr Phe 
    130                 135                 140                 
Asp Glu His Glu Ser Ala Leu Arg Ala Leu Gly Pro Gly Ala Thr Phe 
145                 150                 155                 160 
Val Gly Glu Asp His Gly Leu Ala Asn Leu Tyr Asp Ala Ala Met Ser 
                165                 170                 175     
Ser Val Met Trp Gly Leu Leu Asn Gly Phe Leu His Gly Thr Ala Leu 
            180                 185                 190         
Leu Gly Thr Ala Gly Val Ser Ala Thr Ser Val Val Pro Met Leu Gly 
        195                 200                 205             
Gln Gly Trp Glu Ala Val Thr Gly Leu Ile Ala Arg Tyr Ala Asp Gln 
    210                 215                 220                 
Ile Asp Ala Gly Val Tyr Pro Ala Asp Asp Ala Thr Ile Asp Thr Gln 
225                 230                 235                 240 
Arg Ala Ala Met Gln His Leu Val Glu Glu Ser Arg Ala Ala Gly Ile 
                245                 250                 255     
Asn Gly Glu Leu Pro Ala Phe Val Thr Lys Leu Ala Asp Arg Ala Val 
            260                 265                 270         
Ala Glu Gly Arg Gly Gly Asp Ser Tyr Ala Ala Leu Ile Glu Gln Phe 
        275                 280                 285             
Arg Lys Ser Ala                                                 
    290                                                         

 
<210> 167
<211> 879
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Imine reductase variant from Pseudonocardia spinosispora
 
<400> 167
atgagtgatt cacctgtgcc cgtgacagtt ctgggtcttg gtctgatggg tcaggcctta 60
gcgggagcgt ttctggcagc cggtcaccct acgacggttt ggaatcgcag cgcagctaaa 120
gctgatgaac tggttggtcg cggtgcaacc ctggccgcgt ctcccgcggc tgccgtcgca 180
gcgggtagtc tgatcgttgt ttgcgtcaca gatcatcgtg cggtgcgcga actgctggaa 240
ccacttggcg atgcactgcc aggtcgcgtt ctggtcaacc tgacgagcgg tacgtcgcgt 300
gaagcccgtg agaacgcaga acatactgcg gctcagggag ccacctacct ggatggtgtg 360
attctgacca ttccaagcgg cattggcacc ccagaagcta ggattctgta ttcgggaccg 420
cgcagtacct tcgatgaaca tgaaagcgca ctgcgcgcat taggtccggg ggcgaccttt 480
gtaggggaag atcacggact ggctaacctg tatgatgcag caatgttgag cgtgatgtgg 540
ggcctgctga atggtttcct gcatggaacc gcactgctgg gaaccgcggg cgtttcggca 600
acctctgtgg ttccgatgct gggtcaaggt tgggaagcga tgactggtct aattgcgcgc 660
tacgcagatc agattgatgc cggtgtctat ccagcagacg atgccacgat tgatacccag 720
cgtgctgcaa tgcagcacct ggtcgaggag agccgtgcgg cgggcatcaa tggcgaactt 780
ccggcgtttg tgaccaaact ggccgatcgc gcagtggcag aaggtcgtgg tggtgactct 840
tacgctgcac tgattgaaca gtttcgcaag tcagcttaa                        879

 
<210> 168
<211> 292
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Imine reductase variant from Pseudonocardia spinosispora
 
<400> 168
Met Ser Asp Ser Pro Val Pro Val Thr Val Leu Gly Leu Gly Leu Met 
1               5                   10                  15      
Gly Gln Ala Leu Ala Gly Ala Phe Leu Ala Ala Gly His Pro Thr Thr 
            20                  25                  30          
Val Trp Asn Arg Ser Ala Ala Lys Ala Asp Glu Leu Val Gly Arg Gly 
        35                  40                  45              
Ala Thr Leu Ala Ala Ser Pro Ala Ala Ala Val Ala Ala Gly Ser Leu 
    50                  55                  60                  
Ile Val Val Cys Val Thr Asp His Arg Ala Val Arg Glu Leu Leu Glu 
65                  70                  75                  80  
Pro Leu Gly Asp Ala Leu Pro Gly Arg Val Leu Val Asn Leu Thr Ser 
                85                  90                  95      
Gly Thr Ser Arg Glu Ala Arg Glu Asn Ala Glu His Thr Ala Ala Gln 
            100                 105                 110         
Gly Ala Thr Tyr Leu Asp Gly Val Ile Leu Thr Ile Pro Ser Gly Ile 
        115                 120                 125             
Gly Thr Pro Glu Ala Arg Ile Leu Tyr Ser Gly Pro Arg Ser Thr Phe 
    130                 135                 140                 
Asp Glu His Glu Ser Ala Leu Arg Ala Leu Gly Pro Gly Ala Thr Phe 
145                 150                 155                 160 
Val Gly Glu Asp His Gly Leu Ala Asn Leu Tyr Asp Ala Ala Met Leu 
                165                 170                 175     
Ser Val Met Trp Gly Leu Leu Asn Gly Phe Leu His Gly Thr Ala Leu 
            180                 185                 190         
Leu Gly Thr Ala Gly Val Ser Ala Thr Ser Val Val Pro Met Leu Gly 
        195                 200                 205             
Gln Gly Trp Glu Ala Met Thr Gly Leu Ile Ala Arg Tyr Ala Asp Gln 
    210                 215                 220                 
Ile Asp Ala Gly Val Tyr Pro Ala Asp Asp Ala Thr Ile Asp Thr Gln 
225                 230                 235                 240 
Arg Ala Ala Met Gln His Leu Val Glu Glu Ser Arg Ala Ala Gly Ile 
                245                 250                 255     
Asn Gly Glu Leu Pro Ala Phe Val Thr Lys Leu Ala Asp Arg Ala Val 
            260                 265                 270         
Ala Glu Gly Arg Gly Gly Asp Ser Tyr Ala Ala Leu Ile Glu Gln Phe 
        275                 280                 285             
Arg Lys Ser Ala                                                 
    290                                                         

 
<210> 169
<211> 879
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Imine reductase variant from Pseudonocardia spinosispora
 
<400> 169
atgagtgatt cacctgtgcc cgtgacagtt ctgggtcttg gtctgatggg tcaggcctta 60
gcgggagcgt ttctggcagc cggtcaccct acgacggttt ggaatcgcag cgcagctaaa 120
gctgatgaac tggttggtcg cggtgcaacc ctggccgcgt ctcccgcggc tgccgtcgca 180
gcgggtagtc tgatcgttgt ttgcgtcaca gatcatcgtg cggtgcgcga actgctggaa 240
ccacttggcg atgcactgcc aggtcgcgtt ctggtcaacc tgacgagcgg tacgtcgcgt 300
gaagcccgtg agaacgcaga acatactgcg gctcagggag ccacctacct ggatggtgtg 360
attctgacca ttccaagcgg cattggcacc ccagaagcta ggattctgta ttcgggaccg 420
cgcagtacct tcgatgaaca tgaaagcgca ctgcgcgcat taggtccggg ggcgaccttt 480
gtaggggaag atcacggact ggctaacctg tatgatgcag caatgttgag catgatgtgg 540
ggcctgctga atggtttcct gcatggaacc gcactgctgg gaaccgcggg cgtttcggca 600
acctctgtgg ttccgatgct gggtcaaggt tgggaagcga tgactggtat aattgcgcgc 660
tacgcagatc agattgatgc cggtgtctat ccagcagacg atgccacgat tgatacccag 720
cgtgctgcaa tgcagcacct ggtcgaggag agccgtgcgg cgggcatcaa tggcgaactt 780
ccggcgtttg tgaccaaact ggccgatcgc gcagtggcag aaggtcgtgg tggtgactct 840
tacgctgcac tgattgaaca gtttcgcaag tcagcttaa                        879

 
<210> 170
<211> 292
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Imine reductase variant from Pseudonocardia spinosispora
 
<400> 170
Met Ser Asp Ser Pro Val Pro Val Thr Val Leu Gly Leu Gly Leu Met 
1               5                   10                  15      
Gly Gln Ala Leu Ala Gly Ala Phe Leu Ala Ala Gly His Pro Thr Thr 
            20                  25                  30          
Val Trp Asn Arg Ser Ala Ala Lys Ala Asp Glu Leu Val Gly Arg Gly 
        35                  40                  45              
Ala Thr Leu Ala Ala Ser Pro Ala Ala Ala Val Ala Ala Gly Ser Leu 
    50                  55                  60                  
Ile Val Val Cys Val Thr Asp His Arg Ala Val Arg Glu Leu Leu Glu 
65                  70                  75                  80  
Pro Leu Gly Asp Ala Leu Pro Gly Arg Val Leu Val Asn Leu Thr Ser 
                85                  90                  95      
Gly Thr Ser Arg Glu Ala Arg Glu Asn Ala Glu His Thr Ala Ala Gln 
            100                 105                 110         
Gly Ala Thr Tyr Leu Asp Gly Val Ile Leu Thr Ile Pro Ser Gly Ile 
        115                 120                 125             
Gly Thr Pro Glu Ala Arg Ile Leu Tyr Ser Gly Pro Arg Ser Thr Phe 
    130                 135                 140                 
Asp Glu His Glu Ser Ala Leu Arg Ala Leu Gly Pro Gly Ala Thr Phe 
145                 150                 155                 160 
Val Gly Glu Asp His Gly Leu Ala Asn Leu Tyr Asp Ala Ala Met Leu 
                165                 170                 175     
Ser Met Met Trp Gly Leu Leu Asn Gly Phe Leu His Gly Thr Ala Leu 
            180                 185                 190         
Leu Gly Thr Ala Gly Val Ser Ala Thr Ser Val Val Pro Met Leu Gly 
        195                 200                 205             
Gln Gly Trp Glu Ala Met Thr Gly Ile Ile Ala Arg Tyr Ala Asp Gln 
    210                 215                 220                 
Ile Asp Ala Gly Val Tyr Pro Ala Asp Asp Ala Thr Ile Asp Thr Gln 
225                 230                 235                 240 
Arg Ala Ala Met Gln His Leu Val Glu Glu Ser Arg Ala Ala Gly Ile 
                245                 250                 255     
Asn Gly Glu Leu Pro Ala Phe Val Thr Lys Leu Ala Asp Arg Ala Val 
            260                 265                 270         
Ala Glu Gly Arg Gly Gly Asp Ser Tyr Ala Ala Leu Ile Glu Gln Phe 
        275                 280                 285             
Arg Lys Ser Ala                                                 
    290                                                         

 
<210> 171
<211> 879
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Imine reductase variant from Pseudonocardia spinosispora
 
<400> 171
atgagtgatt cacctgtgcc cgtgacagtt ctgggtcttg gtctgatggg tcaggcctta 60
gcgggagcgt ttctggcagc cggtcaccct acgacggttt ggaatcgcag cgcagctaaa 120
gctgatgaac tggttggtcg cggtgcaacc ctggccgcgt ctcccgcggc tgccgtcgca 180
gcgggtagtc tgatcgttgt ttgcgtcaca gatcatcgtg cggtgcgcga actgctggaa 240
ccacttggcg atgcactgcc aggtcgcgtt ctggtcaacc tgacgagcgg tacgtcgcgt 300
gaagcccgtg agaacgcaga acatactgcg gctcagggag ccacctacct ggatggtgtg 360
attctgacca ttccaagcgg cattggcacc ccagaagcta cgattctgta ttcgggaccg 420
cgcagtacct tcgatgaaca tgaaagcgca ctgcgcgcat taggtccggg ggcgaccttt 480
gtaggggaag atcacggact ggctgccctg tatgatgcag caatgttgag catgatgtgg 540
ggcctgctga atggtttcct gcatggaacc gcactgctgg gaaccgcggg cgtttcggca 600
acctctgtgg ttccgatgct gggtcaaggt tgggaagcgg tgactggtct aattgcgcgc 660
tacgcagatc agattgatgc cggtgtctat ccagcagacg atgccacgat tgatacccag 720
cgtgctgcaa tgcagcacct ggtcgaggag agccgtgcgg cgggcatcaa tggcgaactt 780
ccggcgtttg tgaccaaact ggccgatcgc gcagtggcag aaggtcgtgg tggtgactct 840
tacgctgcac tgattgaaca gtttcgcaag tcagcttaa                        879

 
<210> 172
<211> 292
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Imine reductase variant from Pseudonocardia spinosispora
 
<400> 172
Met Ser Asp Ser Pro Val Pro Val Thr Val Leu Gly Leu Gly Leu Met 
1               5                   10                  15      
Gly Gln Ala Leu Ala Gly Ala Phe Leu Ala Ala Gly His Pro Thr Thr 
            20                  25                  30          
Val Trp Asn Arg Ser Ala Ala Lys Ala Asp Glu Leu Val Gly Arg Gly 
        35                  40                  45              
Ala Thr Leu Ala Ala Ser Pro Ala Ala Ala Val Ala Ala Gly Ser Leu 
    50                  55                  60                  
Ile Val Val Cys Val Thr Asp His Arg Ala Val Arg Glu Leu Leu Glu 
65                  70                  75                  80  
Pro Leu Gly Asp Ala Leu Pro Gly Arg Val Leu Val Asn Leu Thr Ser 
                85                  90                  95      
Gly Thr Ser Arg Glu Ala Arg Glu Asn Ala Glu His Thr Ala Ala Gln 
            100                 105                 110         
Gly Ala Thr Tyr Leu Asp Gly Val Ile Leu Thr Ile Pro Ser Gly Ile 
        115                 120                 125             
Gly Thr Pro Glu Ala Thr Ile Leu Tyr Ser Gly Pro Arg Ser Thr Phe 
    130                 135                 140                 
Asp Glu His Glu Ser Ala Leu Arg Ala Leu Gly Pro Gly Ala Thr Phe 
145                 150                 155                 160 
Val Gly Glu Asp His Gly Leu Ala Ala Leu Tyr Asp Ala Ala Met Leu 
                165                 170                 175     
Ser Met Met Trp Gly Leu Leu Asn Gly Phe Leu His Gly Thr Ala Leu 
            180                 185                 190         
Leu Gly Thr Ala Gly Val Ser Ala Thr Ser Val Val Pro Met Leu Gly 
        195                 200                 205             
Gln Gly Trp Glu Ala Val Thr Gly Leu Ile Ala Arg Tyr Ala Asp Gln 
    210                 215                 220                 
Ile Asp Ala Gly Val Tyr Pro Ala Asp Asp Ala Thr Ile Asp Thr Gln 
225                 230                 235                 240 
Arg Ala Ala Met Gln His Leu Val Glu Glu Ser Arg Ala Ala Gly Ile 
                245                 250                 255     
Asn Gly Glu Leu Pro Ala Phe Val Thr Lys Leu Ala Asp Arg Ala Val 
            260                 265                 270         
Ala Glu Gly Arg Gly Gly Asp Ser Tyr Ala Ala Leu Ile Glu Gln Phe 
        275                 280                 285             
Arg Lys Ser Ala                                                 
    290                                                         

 
<210> 173
<211> 879
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Imine reductase variant from Pseudonocardia spinosispora
 
<400> 173
atgagtgatt cacctgtgcc cgtgacagtt ctgggtcttg gtctgatggg tcaggcctta 60
gcgggagcgt ttctggcagc cggtcaccct acgacggttt ggaatcgcag cgcagctaaa 120
gctgatgaac tggttggtcg cggtgcaacc ctggccgcgt ctcccgcggc tgccgtcgca 180
gcgggtagtc tgatcgttgt ttgcgtcaca gatcatcgtg cggtgcgcga actgctggaa 240
ccacttggcg atgcactgcc aggtcgcgtt ctggtcaacc tgacgagcgg tacgtcgcgt 300
gaagcccgtg agaacgcaga acatactgcg gctcagggag ccacctacct ggatggtgtg 360
attctgacca ttccaagcgg cattggcacc ccagaagcta ggattctgta ttcgggaccg 420
cgcagtacct tcgatgaaca tgaaagcgca ctgcgcgcat taggtccggg ggcgaccttt 480
gtaggggaag atcacggact ggctgccctg tatgatgcag caatgtcgag cgtgatgtgg 540
ggcctgctga atggtttcct gcatggaacc gcactgctgg gaaccgcggg cgtttcggca 600
acctctgtgg ttccgatgct gggtcaaggt tgggaagcgg tgatgggttg gattgcgcgc 660
tacgcagatc agattgatgc cggtgtctat ccagcagacg atgccacgat tgatacccag 720
cgtgctgcaa tgcagcacct ggtcgaggag agccgtgcgg cgggcatcaa tggcgaactt 780
ccggcgtttg tgaccaaact ggccgatcgc gcagtggcag aaggtcgtgg tggtgactct 840
tacgctgcac tgattgaaca gtttcgcaag tcagcttaa                        879

 
<210> 174
<211> 292
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Imine reductase variant from Pseudonocardia spinosispora
 
<400> 174
Met Ser Asp Ser Pro Val Pro Val Thr Val Leu Gly Leu Gly Leu Met 
1               5                   10                  15      
Gly Gln Ala Leu Ala Gly Ala Phe Leu Ala Ala Gly His Pro Thr Thr 
            20                  25                  30          
Val Trp Asn Arg Ser Ala Ala Lys Ala Asp Glu Leu Val Gly Arg Gly 
        35                  40                  45              
Ala Thr Leu Ala Ala Ser Pro Ala Ala Ala Val Ala Ala Gly Ser Leu 
    50                  55                  60                  
Ile Val Val Cys Val Thr Asp His Arg Ala Val Arg Glu Leu Leu Glu 
65                  70                  75                  80  
Pro Leu Gly Asp Ala Leu Pro Gly Arg Val Leu Val Asn Leu Thr Ser 
                85                  90                  95      
Gly Thr Ser Arg Glu Ala Arg Glu Asn Ala Glu His Thr Ala Ala Gln 
            100                 105                 110         
Gly Ala Thr Tyr Leu Asp Gly Val Ile Leu Thr Ile Pro Ser Gly Ile 
        115                 120                 125             
Gly Thr Pro Glu Ala Arg Ile Leu Tyr Ser Gly Pro Arg Ser Thr Phe 
    130                 135                 140                 
Asp Glu His Glu Ser Ala Leu Arg Ala Leu Gly Pro Gly Ala Thr Phe 
145                 150                 155                 160 
Val Gly Glu Asp His Gly Leu Ala Ala Leu Tyr Asp Ala Ala Met Ser 
                165                 170                 175     
Ser Val Met Trp Gly Leu Leu Asn Gly Phe Leu His Gly Thr Ala Leu 
            180                 185                 190         
Leu Gly Thr Ala Gly Val Ser Ala Thr Ser Val Val Pro Met Leu Gly 
        195                 200                 205             
Gln Gly Trp Glu Ala Val Met Gly Trp Ile Ala Arg Tyr Ala Asp Gln 
    210                 215                 220                 
Ile Asp Ala Gly Val Tyr Pro Ala Asp Asp Ala Thr Ile Asp Thr Gln 
225                 230                 235                 240 
Arg Ala Ala Met Gln His Leu Val Glu Glu Ser Arg Ala Ala Gly Ile 
                245                 250                 255     
Asn Gly Glu Leu Pro Ala Phe Val Thr Lys Leu Ala Asp Arg Ala Val 
            260                 265                 270         
Ala Glu Gly Arg Gly Gly Asp Ser Tyr Ala Ala Leu Ile Glu Gln Phe 
        275                 280                 285             
Arg Lys Ser Ala                                                 
    290                                                         

 
<210> 175
<211> 879
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Imine reductase variant from Pseudonocardia spinosispora
 
<400> 175
atgagtgatt cacctgtgcc cgtgacagtt ctgggtcttg gtctgatggg tcaggcctta 60
gcgggagcgt ttctggcagc cggtcaccct acgacggttt ggaatcgcag cgcagctaaa 120
gctgatgaac tggttggtcg cggtgcaacc ctggccgcgt ctcccgcggc tgccgtcgca 180
gcgggtagtc tgatcgttgt ttgcgtcaca gatcatcgtg cggtgcgcga actgctggaa 240
ccacttggcg atgcactgcc aggtcgcgtt ctggtcaacc tgacgagcgg tacgtcgcgt 300
gaagcccgtg agaacgcaga acatactgcg gctcagggag ccacctacct ggatggtgtg 360
attctgacca ttccaagcgg cattggcacc ccagaagcta cgattctgta ttcgggaccg 420
cgcagtacct tcgatgaaca tgaaagcgca ctgcgcgcat taggtccggg ggcgaccttt 480
gtaggggaag atcacggact ggctaacctg tatgatgcag caatgtcgag cgtgatgtgg 540
ggcctgctga atggtttcct gcatggaacc gcactgctgg gaaccgcggg cgtttcggca 600
acctctgtgg ttccgatgct gggtcaaggt tgggaagcga tgactggttg gattgcgcgc 660
tacgcagatc agattgatgc cggtgtctat ccagcagacg atgccacgat tgatacccag 720
cgtgctgcaa tgcagcacct ggtcgaggag agccgtgcgg cgggcatcaa tggcgaactt 780
ccggcgtttg tgaccaaact ggccgatcgc gcagtggcag aaggtcgtgg tggtgactct 840
tacgctgcac tgattgaaca gtttcgcaag tcagcttaa                        879

 
<210> 176
<211> 292
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Imine reductase variant from Pseudonocardia spinosispora
 
<400> 176
Met Ser Asp Ser Pro Val Pro Val Thr Val Leu Gly Leu Gly Leu Met 
1               5                   10                  15      
Gly Gln Ala Leu Ala Gly Ala Phe Leu Ala Ala Gly His Pro Thr Thr 
            20                  25                  30          
Val Trp Asn Arg Ser Ala Ala Lys Ala Asp Glu Leu Val Gly Arg Gly 
        35                  40                  45              
Ala Thr Leu Ala Ala Ser Pro Ala Ala Ala Val Ala Ala Gly Ser Leu 
    50                  55                  60                  
Ile Val Val Cys Val Thr Asp His Arg Ala Val Arg Glu Leu Leu Glu 
65                  70                  75                  80  
Pro Leu Gly Asp Ala Leu Pro Gly Arg Val Leu Val Asn Leu Thr Ser 
                85                  90                  95      
Gly Thr Ser Arg Glu Ala Arg Glu Asn Ala Glu His Thr Ala Ala Gln 
            100                 105                 110         
Gly Ala Thr Tyr Leu Asp Gly Val Ile Leu Thr Ile Pro Ser Gly Ile 
        115                 120                 125             
Gly Thr Pro Glu Ala Thr Ile Leu Tyr Ser Gly Pro Arg Ser Thr Phe 
    130                 135                 140                 
Asp Glu His Glu Ser Ala Leu Arg Ala Leu Gly Pro Gly Ala Thr Phe 
145                 150                 155                 160 
Val Gly Glu Asp His Gly Leu Ala Asn Leu Tyr Asp Ala Ala Met Ser 
                165                 170                 175     
Ser Val Met Trp Gly Leu Leu Asn Gly Phe Leu His Gly Thr Ala Leu 
            180                 185                 190         
Leu Gly Thr Ala Gly Val Ser Ala Thr Ser Val Val Pro Met Leu Gly 
        195                 200                 205             
Gln Gly Trp Glu Ala Met Thr Gly Trp Ile Ala Arg Tyr Ala Asp Gln 
    210                 215                 220                 
Ile Asp Ala Gly Val Tyr Pro Ala Asp Asp Ala Thr Ile Asp Thr Gln 
225                 230                 235                 240 
Arg Ala Ala Met Gln His Leu Val Glu Glu Ser Arg Ala Ala Gly Ile 
                245                 250                 255     
Asn Gly Glu Leu Pro Ala Phe Val Thr Lys Leu Ala Asp Arg Ala Val 
            260                 265                 270         
Ala Glu Gly Arg Gly Gly Asp Ser Tyr Ala Ala Leu Ile Glu Gln Phe 
        275                 280                 285             
Arg Lys Ser Ala                                                 
    290                                                         

 
<210> 177
<211> 879
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Imine reductase variant from Pseudonocardia spinosispora
 
<400> 177
atgagtgatt cacctgtgcc cgtgacagtt ctgggtcttg gtctgatggg tcaggcctta 60
gcgggagcgt ttctggcagc cggtcaccct acgacggttt ggaatcgcag cgcagctaaa 120
gctgatgaac tggttggtcg cggtgcaacc ctggccgcgt ctcccgcggc tgccgtcgca 180
gcgggtagtc tgatcgttgt ttgcgtcaca gatcatcgtg cggtgcgcga actgctggaa 240
ccacttggcg atgcactgcc aggtcgcgtt ctggtcaacc tgacgagcgg tacgtcgcgt 300
gaagcccgtg agaacgcaga acatactgcg gctcagggag ccacctacct ggatggtgtg 360
attctgacca ttccaagcgg cattggcacc ccagaagcta ggattctgta ttcgggaccg 420
cgcagtacct tcgatgaaca tgaaagcgca ctgcgcgcat taggtccggg ggcgaccttt 480
gtaggggaag atcacggact ggctaacctg tatgatgcag caatgtcgag cgttatgtgg 540
ggcctgctga atggtttcct gcatggaacc gcactgctgg gaaccgcggg cgtttcggca 600
acctctgtgg ttccgatgct gggtcaaggt tgggaagcgg tgactggttg gattgcgcgc 660
tacgcagatc agattgatgc cggtgtctat ccagcagacg atgccacgat tgatacccag 720
cgtgctgcaa tgcagcacct ggtcgaggag agccgtgcgg cgggcatcaa tggcgaactt 780
ccggcgtttg tgaccaaact ggccgatcgc gcagtggcag aaggtcgtgg tggtgactct 840
tacgctgcac tgattgaaca gtttcgcaag tcagcttaa                        879

 
<210> 178
<211> 292
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Imine reductase variant from Pseudonocardia spinosispora
 
<400> 178
Met Ser Asp Ser Pro Val Pro Val Thr Val Leu Gly Leu Gly Leu Met 
1               5                   10                  15      
Gly Gln Ala Leu Ala Gly Ala Phe Leu Ala Ala Gly His Pro Thr Thr 
            20                  25                  30          
Val Trp Asn Arg Ser Ala Ala Lys Ala Asp Glu Leu Val Gly Arg Gly 
        35                  40                  45              
Ala Thr Leu Ala Ala Ser Pro Ala Ala Ala Val Ala Ala Gly Ser Leu 
    50                  55                  60                  
Ile Val Val Cys Val Thr Asp His Arg Ala Val Arg Glu Leu Leu Glu 
65                  70                  75                  80  
Pro Leu Gly Asp Ala Leu Pro Gly Arg Val Leu Val Asn Leu Thr Ser 
                85                  90                  95      
Gly Thr Ser Arg Glu Ala Arg Glu Asn Ala Glu His Thr Ala Ala Gln 
            100                 105                 110         
Gly Ala Thr Tyr Leu Asp Gly Val Ile Leu Thr Ile Pro Ser Gly Ile 
        115                 120                 125             
Gly Thr Pro Glu Ala Arg Ile Leu Tyr Ser Gly Pro Arg Ser Thr Phe 
    130                 135                 140                 
Asp Glu His Glu Ser Ala Leu Arg Ala Leu Gly Pro Gly Ala Thr Phe 
145                 150                 155                 160 
Val Gly Glu Asp His Gly Leu Ala Asn Leu Tyr Asp Ala Ala Met Ser 
                165                 170                 175     
Ser Val Met Trp Gly Leu Leu Asn Gly Phe Leu His Gly Thr Ala Leu 
            180                 185                 190         
Leu Gly Thr Ala Gly Val Ser Ala Thr Ser Val Val Pro Met Leu Gly 
        195                 200                 205             
Gln Gly Trp Glu Ala Val Thr Gly Trp Ile Ala Arg Tyr Ala Asp Gln 
    210                 215                 220                 
Ile Asp Ala Gly Val Tyr Pro Ala Asp Asp Ala Thr Ile Asp Thr Gln 
225                 230                 235                 240 
Arg Ala Ala Met Gln His Leu Val Glu Glu Ser Arg Ala Ala Gly Ile 
                245                 250                 255     
Asn Gly Glu Leu Pro Ala Phe Val Thr Lys Leu Ala Asp Arg Ala Val 
            260                 265                 270         
Ala Glu Gly Arg Gly Gly Asp Ser Tyr Ala Ala Leu Ile Glu Gln Phe 
        275                 280                 285             
Arg Lys Ser Ala                                                 
    290                                                         

 
<210> 179
<211> 879
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Imine reductase variant from Pseudonocardia spinosispora
 
<400> 179
atgagtgatt cacctgtgcc cgtgacagtt ctgggtcttg gtctgatggg tcaggcctta 60
gcgggagcgt ttctggcagc cggtcaccct acgacggttt ggaatcgcag cgcagctaaa 120
gctgatgaac tggttggtcg cggtgcaacc ctggccgcgt ctcccgcggc tgccgtcgca 180
gcgggtagtc tgatcgttgt ttgcgtcaca gatcatcgtg cggtgcgcga actgctggaa 240
ccacttggcg atgcactgcc aggtcgcgtt ctggtcaacc tgacgagcgg tacgtcgcgt 300
gaagcccgtg agaacgcaga acatactgcg gctcagggag ccacctacct ggatggtgtg 360
attctgacca ttccaagcgg cattggcacc ccagaagcta cgattctgta ttcgggaccg 420
cgcagtacct tcgatgaaca tgaaagcgca ctgcgcgcat taggtccggg ggcgaccttt 480
gtaggggaag atcacggact ggctaacctg tatgatgcag caatgtcgag cgttatgtgg 540
ggcctgctga atggtttcct gcatggaacc gcactgctgg gaaccgcggg cgtttcggca 600
acctctgtgg ttccgatgct gggtcaaggt atagaagcga tgatgggtat aattgcgcgc 660
tacgcagatc agattgatgc cggtgtctat ccagcagacg atgccacgat tgatacccag 720
cgtgctgcaa tgcagcacct ggtcgaggag agccgtgcgg cgggcatcaa tggcgaactt 780
ccggcgtttg tgaccaaact ggccgatcgc gcagtggcag aaggtcgtgg tggtgactct 840
tacgctgcac tgattgaaca gtttcgcaag tcagcttaa                        879

 
<210> 180
<211> 292
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Imine reductase variant from Pseudonocardia spinosispora
 
<400> 180
Met Ser Asp Ser Pro Val Pro Val Thr Val Leu Gly Leu Gly Leu Met 
1               5                   10                  15      
Gly Gln Ala Leu Ala Gly Ala Phe Leu Ala Ala Gly His Pro Thr Thr 
            20                  25                  30          
Val Trp Asn Arg Ser Ala Ala Lys Ala Asp Glu Leu Val Gly Arg Gly 
        35                  40                  45              
Ala Thr Leu Ala Ala Ser Pro Ala Ala Ala Val Ala Ala Gly Ser Leu 
    50                  55                  60                  
Ile Val Val Cys Val Thr Asp His Arg Ala Val Arg Glu Leu Leu Glu 
65                  70                  75                  80  
Pro Leu Gly Asp Ala Leu Pro Gly Arg Val Leu Val Asn Leu Thr Ser 
                85                  90                  95      
Gly Thr Ser Arg Glu Ala Arg Glu Asn Ala Glu His Thr Ala Ala Gln 
            100                 105                 110         
Gly Ala Thr Tyr Leu Asp Gly Val Ile Leu Thr Ile Pro Ser Gly Ile 
        115                 120                 125             
Gly Thr Pro Glu Ala Thr Ile Leu Tyr Ser Gly Pro Arg Ser Thr Phe 
    130                 135                 140                 
Asp Glu His Glu Ser Ala Leu Arg Ala Leu Gly Pro Gly Ala Thr Phe 
145                 150                 155                 160 
Val Gly Glu Asp His Gly Leu Ala Asn Leu Tyr Asp Ala Ala Met Ser 
                165                 170                 175     
Ser Val Met Trp Gly Leu Leu Asn Gly Phe Leu His Gly Thr Ala Leu 
            180                 185                 190         
Leu Gly Thr Ala Gly Val Ser Ala Thr Ser Val Val Pro Met Leu Gly 
        195                 200                 205             
Gln Gly Ile Glu Ala Met Met Gly Ile Ile Ala Arg Tyr Ala Asp Gln 
    210                 215                 220                 
Ile Asp Ala Gly Val Tyr Pro Ala Asp Asp Ala Thr Ile Asp Thr Gln 
225                 230                 235                 240 
Arg Ala Ala Met Gln His Leu Val Glu Glu Ser Arg Ala Ala Gly Ile 
                245                 250                 255     
Asn Gly Glu Leu Pro Ala Phe Val Thr Lys Leu Ala Asp Arg Ala Val 
            260                 265                 270         
Ala Glu Gly Arg Gly Gly Asp Ser Tyr Ala Ala Leu Ile Glu Gln Phe 
        275                 280                 285             
Arg Lys Ser Ala                                                 
    290                                                         

 
<210> 181
<211> 879
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Imine reductase variant from Pseudonocardia spinosispora
 
<400> 181
atgagtgatt cacctgtgcc cgtgacagtt ctgggtcttg gtctgatggg tcaggcctta 60
gcgggagcgt ttctggcagc cggtcaccct acgacggttt ggaatcgcag cgcagctaaa 120
gctgatgaac tggttggtcg cggtgcaacc ctggccgcgt ctcccgcggc tgccgtcgca 180
gcgggtagtc tgatcgttgt ttgcgtcaca gatcatcgtg cggtgcgcga actgctggaa 240
ccacttggcg atgcactgcc aggtcgcgtt ctggtcaacc tgacgagcgg tacgtcgcgt 300
gaagcccgtg agaacgcaga acatactgcg gctcagggag ccacctacct ggatggtgtg 360
attctgacca ttccaagcgg cattggcacc ccagaagcta cgattctgta ttcgggaccg 420
cgcagtacct tcgatgaaca tgaaagcgca ctgcgcgcat taggtccggg ggcgaccttt 480
gtaggggaag atcacggact ggctaacctg tatgatgcag caatgttgag cgtgatgtgg 540
ggcctgctga atggtttcct gcatggaacc gcactgctgg gaaccgcggg cgtttcggca 600
acctctgtgg ttccgatgct gggtcaaggt tgggaagcgg tgactggtat aattgcgcgc 660
tacgcagatc agattgatgc cggtgtctat ccagcagacg atgccacgat tgatacccag 720
cgtgctgcaa tgcagcacct ggtcgaggag agccgtgcgg cgggcatcaa tggcgaactt 780
ccggcgtttg tgaccaaact ggccgatcgc gcagtggcag aaggtcgtgg tggtgactct 840
tacgctgcac tgattgaaca gtttcgcaag tcagcttaa                        879

 
<210> 182
<211> 292
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Imine reductase variant from Pseudonocardia spinosispora
 
<400> 182
Met Ser Asp Ser Pro Val Pro Val Thr Val Leu Gly Leu Gly Leu Met 
1               5                   10                  15      
Gly Gln Ala Leu Ala Gly Ala Phe Leu Ala Ala Gly His Pro Thr Thr 
            20                  25                  30          
Val Trp Asn Arg Ser Ala Ala Lys Ala Asp Glu Leu Val Gly Arg Gly 
        35                  40                  45              
Ala Thr Leu Ala Ala Ser Pro Ala Ala Ala Val Ala Ala Gly Ser Leu 
    50                  55                  60                  
Ile Val Val Cys Val Thr Asp His Arg Ala Val Arg Glu Leu Leu Glu 
65                  70                  75                  80  
Pro Leu Gly Asp Ala Leu Pro Gly Arg Val Leu Val Asn Leu Thr Ser 
                85                  90                  95      
Gly Thr Ser Arg Glu Ala Arg Glu Asn Ala Glu His Thr Ala Ala Gln 
            100                 105                 110         
Gly Ala Thr Tyr Leu Asp Gly Val Ile Leu Thr Ile Pro Ser Gly Ile 
        115                 120                 125             
Gly Thr Pro Glu Ala Thr Ile Leu Tyr Ser Gly Pro Arg Ser Thr Phe 
    130                 135                 140                 
Asp Glu His Glu Ser Ala Leu Arg Ala Leu Gly Pro Gly Ala Thr Phe 
145                 150                 155                 160 
Val Gly Glu Asp His Gly Leu Ala Asn Leu Tyr Asp Ala Ala Met Leu 
                165                 170                 175     
Ser Val Met Trp Gly Leu Leu Asn Gly Phe Leu His Gly Thr Ala Leu 
            180                 185                 190         
Leu Gly Thr Ala Gly Val Ser Ala Thr Ser Val Val Pro Met Leu Gly 
        195                 200                 205             
Gln Gly Trp Glu Ala Val Thr Gly Ile Ile Ala Arg Tyr Ala Asp Gln 
    210                 215                 220                 
Ile Asp Ala Gly Val Tyr Pro Ala Asp Asp Ala Thr Ile Asp Thr Gln 
225                 230                 235                 240 
Arg Ala Ala Met Gln His Leu Val Glu Glu Ser Arg Ala Ala Gly Ile 
                245                 250                 255     
Asn Gly Glu Leu Pro Ala Phe Val Thr Lys Leu Ala Asp Arg Ala Val 
            260                 265                 270         
Ala Glu Gly Arg Gly Gly Asp Ser Tyr Ala Ala Leu Ile Glu Gln Phe 
        275                 280                 285             
Arg Lys Ser Ala                                                 
    290                                                         

 
<210> 183
<211> 879
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Imine reductase variant from Pseudonocardia spinosispora
 
<400> 183
atgagtgatt cacctgtgcc cgtgacagtt ctgggtcttg gtctgatggg tcaggcctta 60
gcgggagcgt ttctggcagc cggtcaccct acgacggttt ggaatcgcag cgcagctaaa 120
gctgatgaac tggttggtcg cggtgcaacc ctggccgcgt ctcccgcggc tgccgtcgca 180
gcgggtagtc tgatcgttgt ttgcgtcaca gatcatcgtg cggtgcgcga actgctggaa 240
ccacttggcg atgcactgcc aggtcgcgtt ctggtcaacc tgacgagcgg tacgtcgcgt 300
gaagcccgtg agaacgcaga acatactgcg gctcagggag ccacctacct ggatggtgtg 360
attctgacca ttccaagcgg cattggcacc ccagaagcta ggattctgta ttcgggaccg 420
cgcagtacct tcgatgaaca tgaaagcgca ctgcgcgcat taggtccggg ggcgaccttt 480
gtaggggaag atcacggact ggctgcactg tatgatgcag caatgttgag cgtgatgtgg 540
ggcctgctga atggtttcct gcatggaacc gcactgctgg gaaccgcggg cgtttcggca 600
acctctgtgg ttccgatgct gggtcaaggt tgggaagcga tgactggttg gattgcgcgc 660
tacgcagatc agattgatgc cggtgtctat ccagcagacg atgccacgat tgatacccag 720
cgtgctgcaa tgcagcacct ggtcgaggag agccgtgcgg cgggcatcaa tggcgaactt 780
ccggcgtttg tgaccaaact ggccgatcgc gcagtggcag aaggtcgtgg tggtgactct 840
tacgctgcac tgattgaaca gtttcgcaag tcagcttaa                        879

 
<210> 184
<211> 292
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Imine reductase variant from Pseudonocardia spinosispora
 
<400> 184
Met Ser Asp Ser Pro Val Pro Val Thr Val Leu Gly Leu Gly Leu Met 
1               5                   10                  15      
Gly Gln Ala Leu Ala Gly Ala Phe Leu Ala Ala Gly His Pro Thr Thr 
            20                  25                  30          
Val Trp Asn Arg Ser Ala Ala Lys Ala Asp Glu Leu Val Gly Arg Gly 
        35                  40                  45              
Ala Thr Leu Ala Ala Ser Pro Ala Ala Ala Val Ala Ala Gly Ser Leu 
    50                  55                  60                  
Ile Val Val Cys Val Thr Asp His Arg Ala Val Arg Glu Leu Leu Glu 
65                  70                  75                  80  
Pro Leu Gly Asp Ala Leu Pro Gly Arg Val Leu Val Asn Leu Thr Ser 
                85                  90                  95      
Gly Thr Ser Arg Glu Ala Arg Glu Asn Ala Glu His Thr Ala Ala Gln 
            100                 105                 110         
Gly Ala Thr Tyr Leu Asp Gly Val Ile Leu Thr Ile Pro Ser Gly Ile 
        115                 120                 125             
Gly Thr Pro Glu Ala Arg Ile Leu Tyr Ser Gly Pro Arg Ser Thr Phe 
    130                 135                 140                 
Asp Glu His Glu Ser Ala Leu Arg Ala Leu Gly Pro Gly Ala Thr Phe 
145                 150                 155                 160 
Val Gly Glu Asp His Gly Leu Ala Ala Leu Tyr Asp Ala Ala Met Leu 
                165                 170                 175     
Ser Val Met Trp Gly Leu Leu Asn Gly Phe Leu His Gly Thr Ala Leu 
            180                 185                 190         
Leu Gly Thr Ala Gly Val Ser Ala Thr Ser Val Val Pro Met Leu Gly 
        195                 200                 205             
Gln Gly Trp Glu Ala Met Thr Gly Trp Ile Ala Arg Tyr Ala Asp Gln 
    210                 215                 220                 
Ile Asp Ala Gly Val Tyr Pro Ala Asp Asp Ala Thr Ile Asp Thr Gln 
225                 230                 235                 240 
Arg Ala Ala Met Gln His Leu Val Glu Glu Ser Arg Ala Ala Gly Ile 
                245                 250                 255     
Asn Gly Glu Leu Pro Ala Phe Val Thr Lys Leu Ala Asp Arg Ala Val 
            260                 265                 270         
Ala Glu Gly Arg Gly Gly Asp Ser Tyr Ala Ala Leu Ile Glu Gln Phe 
        275                 280                 285             
Arg Lys Ser Ala                                                 
    290                                                         

 
<210> 185
<211> 879
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Imine reductase variant from Pseudonocardia spinosispora
 
<400> 185
atgagtgatt cacctgtgcc cgtgacagtt ctgggtcttg gtctgatggg tcaggcctta 60
gcgggagcgt ttctggcagc cggtcaccct acgacggttt ggaatcgcag cgcagctaaa 120
gctgatgaac tggttggtcg cggtgcaacc ctggccgcgt ctcccgcggc tgccgtcgca 180
gcgggtagtc tgatcgttgt ttgcgtcaca gatcatcgtg cggtgcgcga actgctggaa 240
ccacttggcg atgcactgcc aggtcgcgtt ctggtcaacc tgacgagcgg tacgtcgcgt 300
gaagcccgtg agaacgcaga acatactgcg gctcagggag ccacctacct ggatggtgtg 360
attctgacca ttccaagcgg cattggcacc ccagaagcta ggattctgta ttcgggaccg 420
cgcagtacct tcgatgaaca tgaaagcgca ctgcgcgcat taggtccggg ggcgaccttt 480
gtaggggaag atcacggact ggctgccctg tatgatgcag caatgtcgag cgtgatgtgg 540
ggcctgctga atggtttcct gcatggaacc gcactgctgg gaaccgcggg cgtttcggca 600
acctctgtgg ttccgatgct gggtcaaggt tgggaagcga tgactggttg gattgcgcgc 660
tacgcagatc agattgatgc cggtgtctat ccagcagacg atgccacgat tgatacccag 720
cgtgctgcaa tgcagcacct ggtcgaggag agccgtgcgg cgggcatcaa tggcgaactt 780
ccggcgtttg tgaccaaact ggccgatcgc gcagtggcag aaggtcgtgg tggtgactct 840
tacgctgcac tgattgaaca gtttcgcaag tcagcttaa                        879

 
<210> 186
<211> 292
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Imine reductase variant from Pseudonocardia spinosispora
 
<400> 186
Met Ser Asp Ser Pro Val Pro Val Thr Val Leu Gly Leu Gly Leu Met 
1               5                   10                  15      
Gly Gln Ala Leu Ala Gly Ala Phe Leu Ala Ala Gly His Pro Thr Thr 
            20                  25                  30          
Val Trp Asn Arg Ser Ala Ala Lys Ala Asp Glu Leu Val Gly Arg Gly 
        35                  40                  45              
Ala Thr Leu Ala Ala Ser Pro Ala Ala Ala Val Ala Ala Gly Ser Leu 
    50                  55                  60                  
Ile Val Val Cys Val Thr Asp His Arg Ala Val Arg Glu Leu Leu Glu 
65                  70                  75                  80  
Pro Leu Gly Asp Ala Leu Pro Gly Arg Val Leu Val Asn Leu Thr Ser 
                85                  90                  95      
Gly Thr Ser Arg Glu Ala Arg Glu Asn Ala Glu His Thr Ala Ala Gln 
            100                 105                 110         
Gly Ala Thr Tyr Leu Asp Gly Val Ile Leu Thr Ile Pro Ser Gly Ile 
        115                 120                 125             
Gly Thr Pro Glu Ala Arg Ile Leu Tyr Ser Gly Pro Arg Ser Thr Phe 
    130                 135                 140                 
Asp Glu His Glu Ser Ala Leu Arg Ala Leu Gly Pro Gly Ala Thr Phe 
145                 150                 155                 160 
Val Gly Glu Asp His Gly Leu Ala Ala Leu Tyr Asp Ala Ala Met Ser 
                165                 170                 175     
Ser Val Met Trp Gly Leu Leu Asn Gly Phe Leu His Gly Thr Ala Leu 
            180                 185                 190         
Leu Gly Thr Ala Gly Val Ser Ala Thr Ser Val Val Pro Met Leu Gly 
        195                 200                 205             
Gln Gly Trp Glu Ala Met Thr Gly Trp Ile Ala Arg Tyr Ala Asp Gln 
    210                 215                 220                 
Ile Asp Ala Gly Val Tyr Pro Ala Asp Asp Ala Thr Ile Asp Thr Gln 
225                 230                 235                 240 
Arg Ala Ala Met Gln His Leu Val Glu Glu Ser Arg Ala Ala Gly Ile 
                245                 250                 255     
Asn Gly Glu Leu Pro Ala Phe Val Thr Lys Leu Ala Asp Arg Ala Val 
            260                 265                 270         
Ala Glu Gly Arg Gly Gly Asp Ser Tyr Ala Ala Leu Ile Glu Gln Phe 
        275                 280                 285             
Arg Lys Ser Ala                                                 
    290                                                         

 
<210> 187
<211> 879
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Imine reductase variant from Pseudonocardia spinosispora
 
<400> 187
atgagtgatt cacctgtgcc cgtgacagtt ctgggtcttg gtctgatggg tcaggcctta 60
gcgggagcgt ttctggcagc cggtcaccct acgacggttt ggaatcgcag cgcagctaaa 120
gctgatgaac tggttggtcg cggtgcaacc ctggccgcgt ctcccgcggc tgccgtcgca 180
gcgggtagtc tgatcgttgt ttgcgtcaca gatcatcgtg cggtgcgcga actgctggaa 240
ccacttggcg atgcactgcc aggtcgcgtt ctggtcaacc tgacgagcgg tacgtcgcgt 300
gaagcccgtg agaacgcaga acatactgcg gctcagggag ccacctacct ggatggtgtg 360
attctgacca ttccaagcgg cattggcacc ccagaagcta cgattctgta ttcgggaccg 420
cgcagtacct tcgatgaaca tgaaagcgca ctgcgcgcat taggtccggg ggcgaccttt 480
gtaggggaag atcacggact ggctaacctg tatgatgcag caatgtcgag cgtgatgtgg 540
ggcctgctga atggtttcct gcatggaacc gcactgctgg gaaccgcggg cgtttcggca 600
acctctgtgg ttccgatgct gggtcaaggt ctagaagcga tgactggtat aattgcgcgc 660
tacgcagatc agattgatgc cggtgtctat ccagcagacg atgccacgat tgatacccag 720
cgtgctgcaa tgcagcacct ggtcgaggag agccgtgcgg cgggcatcaa tggcgaactt 780
ccggcgtttg tgaccaaact ggccgatcgc gcagtggcag aaggtcgtgg tggtgactct 840
tacgctgcac tgattgaaca gtttcgcaag tcagcttaa                        879

 
<210> 188
<211> 292
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Imine reductase variant from Pseudonocardia spinosispora
 
<400> 188
Met Ser Asp Ser Pro Val Pro Val Thr Val Leu Gly Leu Gly Leu Met 
1               5                   10                  15      
Gly Gln Ala Leu Ala Gly Ala Phe Leu Ala Ala Gly His Pro Thr Thr 
            20                  25                  30          
Val Trp Asn Arg Ser Ala Ala Lys Ala Asp Glu Leu Val Gly Arg Gly 
        35                  40                  45              
Ala Thr Leu Ala Ala Ser Pro Ala Ala Ala Val Ala Ala Gly Ser Leu 
    50                  55                  60                  
Ile Val Val Cys Val Thr Asp His Arg Ala Val Arg Glu Leu Leu Glu 
65                  70                  75                  80  
Pro Leu Gly Asp Ala Leu Pro Gly Arg Val Leu Val Asn Leu Thr Ser 
                85                  90                  95      
Gly Thr Ser Arg Glu Ala Arg Glu Asn Ala Glu His Thr Ala Ala Gln 
            100                 105                 110         
Gly Ala Thr Tyr Leu Asp Gly Val Ile Leu Thr Ile Pro Ser Gly Ile 
        115                 120                 125             
Gly Thr Pro Glu Ala Thr Ile Leu Tyr Ser Gly Pro Arg Ser Thr Phe 
    130                 135                 140                 
Asp Glu His Glu Ser Ala Leu Arg Ala Leu Gly Pro Gly Ala Thr Phe 
145                 150                 155                 160 
Val Gly Glu Asp His Gly Leu Ala Asn Leu Tyr Asp Ala Ala Met Ser 
                165                 170                 175     
Ser Val Met Trp Gly Leu Leu Asn Gly Phe Leu His Gly Thr Ala Leu 
            180                 185                 190         
Leu Gly Thr Ala Gly Val Ser Ala Thr Ser Val Val Pro Met Leu Gly 
        195                 200                 205             
Gln Gly Leu Glu Ala Met Thr Gly Ile Ile Ala Arg Tyr Ala Asp Gln 
    210                 215                 220                 
Ile Asp Ala Gly Val Tyr Pro Ala Asp Asp Ala Thr Ile Asp Thr Gln 
225                 230                 235                 240 
Arg Ala Ala Met Gln His Leu Val Glu Glu Ser Arg Ala Ala Gly Ile 
                245                 250                 255     
Asn Gly Glu Leu Pro Ala Phe Val Thr Lys Leu Ala Asp Arg Ala Val 
            260                 265                 270         
Ala Glu Gly Arg Gly Gly Asp Ser Tyr Ala Ala Leu Ile Glu Gln Phe 
        275                 280                 285             
Arg Lys Ser Ala                                                 
    290                                                         

 
<210> 189
<211> 879
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Imine reductase variant from Pseudonocardia spinosispora
 
<400> 189
atgagtgatt cacctgtgcc cgtgacagtt ctgggtcttg gtctgatggg tcaggcctta 60
gcgggagcgt ttctggcagc cggtcaccct acgacggttt ggaatcgcag cgcagctaaa 120
gctgatgaac tggttggtcg cggtgcaacc ctggccgcgt ctcccgcggc tgccgtcgca 180
gcgggtagtc tgatcgttgt ttgcgtcaca gatcatcgtg cggtgcgcga actgctggaa 240
ccacttggcg atgcactgcc aggtcgcgtt ctggtcaacc tgacgagcgg tacgtcgcgt 300
gaagcccgtg agaacgcaga acatactgcg gctcagggag ccacctacct ggatggtgtg 360
attctgacca ttccaagcgg cattggcacc ccagaagcta cgattctgta ttcgggaccg 420
cgcagtacct tcgatgaaca tgaaagcgca ctgcgcgcat taggtccggg ggcgaccttt 480
gtaggggaag atcacggact ggctaacctg tatgatgcag caatgttgag catgatgtgg 540
ggcctgctga atggtttcct gcatggaacc gcactgctgg gaaccgcggg cgtttcggca 600
acctctgtgg ttccgatgct gggtcaaggt atagaagcgg tgactggttg gattgcgcgc 660
tacgcagatc agattgatgc cggtgtctat ccagcagacc tcgccacgat tgatacccag 720
cgtgctgcaa tgcagcacct ggtcgaggag agccgtgcgg cgggcatcaa tggcgaactt 780
ccggcgtttg tgaccaaact ggccgatcgc gcagtggcag aaggtcgtgg tggtgactct 840
tacgctgcac tgattgaaca gtttcgcaag tcagcttaa                        879

 
<210> 190
<211> 292
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Imine reductase variant from Pseudonocardia spinosispora
 
<400> 190
Met Ser Asp Ser Pro Val Pro Val Thr Val Leu Gly Leu Gly Leu Met 
1               5                   10                  15      
Gly Gln Ala Leu Ala Gly Ala Phe Leu Ala Ala Gly His Pro Thr Thr 
            20                  25                  30          
Val Trp Asn Arg Ser Ala Ala Lys Ala Asp Glu Leu Val Gly Arg Gly 
        35                  40                  45              
Ala Thr Leu Ala Ala Ser Pro Ala Ala Ala Val Ala Ala Gly Ser Leu 
    50                  55                  60                  
Ile Val Val Cys Val Thr Asp His Arg Ala Val Arg Glu Leu Leu Glu 
65                  70                  75                  80  
Pro Leu Gly Asp Ala Leu Pro Gly Arg Val Leu Val Asn Leu Thr Ser 
                85                  90                  95      
Gly Thr Ser Arg Glu Ala Arg Glu Asn Ala Glu His Thr Ala Ala Gln 
            100                 105                 110         
Gly Ala Thr Tyr Leu Asp Gly Val Ile Leu Thr Ile Pro Ser Gly Ile 
        115                 120                 125             
Gly Thr Pro Glu Ala Thr Ile Leu Tyr Ser Gly Pro Arg Ser Thr Phe 
    130                 135                 140                 
Asp Glu His Glu Ser Ala Leu Arg Ala Leu Gly Pro Gly Ala Thr Phe 
145                 150                 155                 160 
Val Gly Glu Asp His Gly Leu Ala Asn Leu Tyr Asp Ala Ala Met Leu 
                165                 170                 175     
Ser Met Met Trp Gly Leu Leu Asn Gly Phe Leu His Gly Thr Ala Leu 
            180                 185                 190         
Leu Gly Thr Ala Gly Val Ser Ala Thr Ser Val Val Pro Met Leu Gly 
        195                 200                 205             
Gln Gly Ile Glu Ala Val Thr Gly Trp Ile Ala Arg Tyr Ala Asp Gln 
    210                 215                 220                 
Ile Asp Ala Gly Val Tyr Pro Ala Asp Leu Ala Thr Ile Asp Thr Gln 
225                 230                 235                 240 
Arg Ala Ala Met Gln His Leu Val Glu Glu Ser Arg Ala Ala Gly Ile 
                245                 250                 255     
Asn Gly Glu Leu Pro Ala Phe Val Thr Lys Leu Ala Asp Arg Ala Val 
            260                 265                 270         
Ala Glu Gly Arg Gly Gly Asp Ser Tyr Ala Ala Leu Ile Glu Gln Phe 
        275                 280                 285             
Arg Lys Ser Ala                                                 
    290                                                         

 
<210> 191
<211> 879
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Imine reductase variant from Pseudonocardia spinosispora
 
<400> 191
atgagtgatt cacctgtgcc cgtgacagtt ctgggtcttg gtctgatggg tcaggcctta 60
gcgggagcgt ttctggcagc cggtcaccct acgacggttt ggaatcgcag cgcagctaaa 120
gctgatgaac tggttggtcg cggtgcaacc ctggccgcgt ctcccgcggc tgccgtcgca 180
gcgggtagtc tgatcgttgt ttgcgtcaca gatcatcgtg cggtgcgcga actgctggaa 240
ccacttggcg atgcactgcc aggtcgcgtt ctggtcaacc tgacgagcgg tacgtcgcgt 300
gaagcccgtg agaacgcaga acatactgcg gctcagggag ccacctacct ggatggtgtg 360
attctgacca ttccaagcgg cattggcacc ccagaagcta ccattctgta ttcgggaccg 420
cgcagtacct tcgatgaaca tgaaagcgca ctgcgcgcat taggtccggg ggcgaccttt 480
gtaggggaag atcacggact ggctaacctg tatgatgcag caatgtcgag cattatgtgg 540
ggcctgctga atggtttcct gcatggaacc gcactgctgg gaaccgcggg cgtttcggca 600
acctctgtgg ttccgatgct gggtcaaggt tgggaagcgg tgactggtct aattgcgcgc 660
tacgcagatc agattgatgc cggtgtctat ccagcagacg atgccacgat tgatacccag 720
cgtgctgcaa tgcagcacct ggtcgaggag agccgtgcgg cgggcatcaa tggcgaactt 780
ccggcgtttg tgaccaaact ggccgatcgc gcagtggcag aaggtcgtgg tggtgactct 840
tacgctgcac tgattgaaca gtttcgcaag tcagcttaa                        879

 
<210> 192
<211> 292
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Imine reductase variant from Pseudonocardia spinosispora
 
<400> 192
Met Ser Asp Ser Pro Val Pro Val Thr Val Leu Gly Leu Gly Leu Met 
1               5                   10                  15      
Gly Gln Ala Leu Ala Gly Ala Phe Leu Ala Ala Gly His Pro Thr Thr 
            20                  25                  30          
Val Trp Asn Arg Ser Ala Ala Lys Ala Asp Glu Leu Val Gly Arg Gly 
        35                  40                  45              
Ala Thr Leu Ala Ala Ser Pro Ala Ala Ala Val Ala Ala Gly Ser Leu 
    50                  55                  60                  
Ile Val Val Cys Val Thr Asp His Arg Ala Val Arg Glu Leu Leu Glu 
65                  70                  75                  80  
Pro Leu Gly Asp Ala Leu Pro Gly Arg Val Leu Val Asn Leu Thr Ser 
                85                  90                  95      
Gly Thr Ser Arg Glu Ala Arg Glu Asn Ala Glu His Thr Ala Ala Gln 
            100                 105                 110         
Gly Ala Thr Tyr Leu Asp Gly Val Ile Leu Thr Ile Pro Ser Gly Ile 
        115                 120                 125             
Gly Thr Pro Glu Ala Thr Ile Leu Tyr Ser Gly Pro Arg Ser Thr Phe 
    130                 135                 140                 
Asp Glu His Glu Ser Ala Leu Arg Ala Leu Gly Pro Gly Ala Thr Phe 
145                 150                 155                 160 
Val Gly Glu Asp His Gly Leu Ala Asn Leu Tyr Asp Ala Ala Met Ser 
                165                 170                 175     
Ser Ile Met Trp Gly Leu Leu Asn Gly Phe Leu His Gly Thr Ala Leu 
            180                 185                 190         
Leu Gly Thr Ala Gly Val Ser Ala Thr Ser Val Val Pro Met Leu Gly 
        195                 200                 205             
Gln Gly Trp Glu Ala Val Thr Gly Leu Ile Ala Arg Tyr Ala Asp Gln 
    210                 215                 220                 
Ile Asp Ala Gly Val Tyr Pro Ala Asp Asp Ala Thr Ile Asp Thr Gln 
225                 230                 235                 240 
Arg Ala Ala Met Gln His Leu Val Glu Glu Ser Arg Ala Ala Gly Ile 
                245                 250                 255     
Asn Gly Glu Leu Pro Ala Phe Val Thr Lys Leu Ala Asp Arg Ala Val 
            260                 265                 270         
Ala Glu Gly Arg Gly Gly Asp Ser Tyr Ala Ala Leu Ile Glu Gln Phe 
        275                 280                 285             
Arg Lys Ser Ala                                                 
    290                                                         

 
<210> 193
<211> 879
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Imine reductase variant from Pseudonocardia spinosispora
 
<400> 193
atgagtgatt cacctgtgcc cgtgacagtt ctgggtcttg gtctgatggg tcaggcctta 60
gcgggagcgt ttctggcagc cggtcaccct acgacggttt ggaatcgcag cgcagctaaa 120
gctgatgaac tggttggtcg cggtgcaacc ctggccgcgt ctcccgcggc tgccgtcgca 180
gcgggtagtc tgatcgttgt ttgcgtcaca gatcatcgtg cggtgcgcga actgctggaa 240
ccacttggcg atgcactgcc aggtcgcgtt ctggtcaacc tgacgagcgg tacgtcgcgt 300
gaagcccgtg agaacgcaga acatactgcg gctcagggag ccacctacct ggatggtgtg 360
attctgacca ttccaagcgg cattggcacc ccagaagcta cgattctgta ttcgggaccg 420
cgcagtacct tcgatgaaca tgaaagcgca ctgcgcgcat taggtccggg ggcgaccttt 480
gtaggggaag atcacggact ggctaacctg tatgatgcag caatgtcgag cattatgtgg 540
ggcctgctga atggtttcct gcatggaacc gcactgctgg gaaccgcggg cgtttcggca 600
acctctgtgg ttccgatgct gggtcaaggt tgggaagcga tgactggtct aattgcgcgc 660
tacgcagatc agattgatgc cggtgtctat ccagcagacg atgccacgat tgatacccag 720
cgtgctgcaa tgcagcacct ggtcgaggag agccgtgcgg cgggcatcaa tggcgaactt 780
ccggcgtttg tgaccaaact ggccgatcgc gcagtggcag aaggtcgtgg tggtgactct 840
tacgctgcac tgattgaaca gtttcgcaag tcagcttaa                        879

 
<210> 194
<211> 292
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Imine reductase variant from Pseudonocardia spinosispora
 
<400> 194
Met Ser Asp Ser Pro Val Pro Val Thr Val Leu Gly Leu Gly Leu Met 
1               5                   10                  15      
Gly Gln Ala Leu Ala Gly Ala Phe Leu Ala Ala Gly His Pro Thr Thr 
            20                  25                  30          
Val Trp Asn Arg Ser Ala Ala Lys Ala Asp Glu Leu Val Gly Arg Gly 
        35                  40                  45              
Ala Thr Leu Ala Ala Ser Pro Ala Ala Ala Val Ala Ala Gly Ser Leu 
    50                  55                  60                  
Ile Val Val Cys Val Thr Asp His Arg Ala Val Arg Glu Leu Leu Glu 
65                  70                  75                  80  
Pro Leu Gly Asp Ala Leu Pro Gly Arg Val Leu Val Asn Leu Thr Ser 
                85                  90                  95      
Gly Thr Ser Arg Glu Ala Arg Glu Asn Ala Glu His Thr Ala Ala Gln 
            100                 105                 110         
Gly Ala Thr Tyr Leu Asp Gly Val Ile Leu Thr Ile Pro Ser Gly Ile 
        115                 120                 125             
Gly Thr Pro Glu Ala Thr Ile Leu Tyr Ser Gly Pro Arg Ser Thr Phe 
    130                 135                 140                 
Asp Glu His Glu Ser Ala Leu Arg Ala Leu Gly Pro Gly Ala Thr Phe 
145                 150                 155                 160 
Val Gly Glu Asp His Gly Leu Ala Asn Leu Tyr Asp Ala Ala Met Ser 
                165                 170                 175     
Ser Ile Met Trp Gly Leu Leu Asn Gly Phe Leu His Gly Thr Ala Leu 
            180                 185                 190         
Leu Gly Thr Ala Gly Val Ser Ala Thr Ser Val Val Pro Met Leu Gly 
        195                 200                 205             
Gln Gly Trp Glu Ala Met Thr Gly Leu Ile Ala Arg Tyr Ala Asp Gln 
    210                 215                 220                 
Ile Asp Ala Gly Val Tyr Pro Ala Asp Asp Ala Thr Ile Asp Thr Gln 
225                 230                 235                 240 
Arg Ala Ala Met Gln His Leu Val Glu Glu Ser Arg Ala Ala Gly Ile 
                245                 250                 255     
Asn Gly Glu Leu Pro Ala Phe Val Thr Lys Leu Ala Asp Arg Ala Val 
            260                 265                 270         
Ala Glu Gly Arg Gly Gly Asp Ser Tyr Ala Ala Leu Ile Glu Gln Phe 
        275                 280                 285             
Arg Lys Ser Ala                                                 
    290                                                         

 
<210> 195
<211> 879
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Imine reductase variant from Pseudonocardia spinosispora
 
<400> 195
atgagtgatt cacctgtgcc cgtgacagtt ctgggtcttg gtctgatggg tcaggcctta 60
gcgggagcgt ttctggcagc cggtcaccct acgacggttt ggaatcgcag cgcagctaaa 120
gctgatgaac tggttggtcg cggtgcaacc ctggccgcgt ctcccgcggc tgccgtcgca 180
gcgggtagtc tgatcgttgt ttgcgtcaca gatcatcgtg cggtgcgcga actgctggaa 240
ccacttggcg atgcactgcc aggtcgcgtt ctggtcaacc tgacgagcgg tacgtcgcgt 300
gaagcccgtg agaacgcaga acatactgcg gctcagggag ccacctacct ggatggtgtg 360
attctgacca ttccaagcgg cattggcacc ccagaagcta ccattctgta ttcgggaccg 420
cgcagtacct tcgatgaaca tgaaagcgca ctgcgcgcat taggtccggg ggcgaccttt 480
gtaggggaag atcacggact ggctgcactg tatgatgcag caatgctgag cgtcatgtgg 540
ggcctgctga atggtttcct gcatggaacc gcactgctgg gaaccgcggg cgtttcggca 600
acctctgtgg ttccgatgct gggtcaaggt tgggaagcgg tgactggttg gattgcgcgc 660
tacgcagatc agattgatgc cggtgtctat ccagcagacc tcgccacgat tgatacccag 720
cgtgctgcaa tgcagcacct ggtcgaggag agccgtgcgg cgggcatcaa tggcgaactt 780
ccggcgtttg tgaccaaact ggccgatcgc gcagtggcag aaggtcgtgg tggtgactct 840
tacgctgcac tgattgaaca gtttcgcaag tcagcttaa                        879

 
<210> 196
<211> 292
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Imine reductase variant from Pseudonocardia spinosispora
 
<400> 196
Met Ser Asp Ser Pro Val Pro Val Thr Val Leu Gly Leu Gly Leu Met 
1               5                   10                  15      
Gly Gln Ala Leu Ala Gly Ala Phe Leu Ala Ala Gly His Pro Thr Thr 
            20                  25                  30          
Val Trp Asn Arg Ser Ala Ala Lys Ala Asp Glu Leu Val Gly Arg Gly 
        35                  40                  45              
Ala Thr Leu Ala Ala Ser Pro Ala Ala Ala Val Ala Ala Gly Ser Leu 
    50                  55                  60                  
Ile Val Val Cys Val Thr Asp His Arg Ala Val Arg Glu Leu Leu Glu 
65                  70                  75                  80  
Pro Leu Gly Asp Ala Leu Pro Gly Arg Val Leu Val Asn Leu Thr Ser 
                85                  90                  95      
Gly Thr Ser Arg Glu Ala Arg Glu Asn Ala Glu His Thr Ala Ala Gln 
            100                 105                 110         
Gly Ala Thr Tyr Leu Asp Gly Val Ile Leu Thr Ile Pro Ser Gly Ile 
        115                 120                 125             
Gly Thr Pro Glu Ala Thr Ile Leu Tyr Ser Gly Pro Arg Ser Thr Phe 
    130                 135                 140                 
Asp Glu His Glu Ser Ala Leu Arg Ala Leu Gly Pro Gly Ala Thr Phe 
145                 150                 155                 160 
Val Gly Glu Asp His Gly Leu Ala Ala Leu Tyr Asp Ala Ala Met Leu 
                165                 170                 175     
Ser Val Met Trp Gly Leu Leu Asn Gly Phe Leu His Gly Thr Ala Leu 
            180                 185                 190         
Leu Gly Thr Ala Gly Val Ser Ala Thr Ser Val Val Pro Met Leu Gly 
        195                 200                 205             
Gln Gly Trp Glu Ala Val Thr Gly Trp Ile Ala Arg Tyr Ala Asp Gln 
    210                 215                 220                 
Ile Asp Ala Gly Val Tyr Pro Ala Asp Leu Ala Thr Ile Asp Thr Gln 
225                 230                 235                 240 
Arg Ala Ala Met Gln His Leu Val Glu Glu Ser Arg Ala Ala Gly Ile 
                245                 250                 255     
Asn Gly Glu Leu Pro Ala Phe Val Thr Lys Leu Ala Asp Arg Ala Val 
            260                 265                 270         
Ala Glu Gly Arg Gly Gly Asp Ser Tyr Ala Ala Leu Ile Glu Gln Phe 
        275                 280                 285             
Arg Lys Ser Ala                                                 
    290                                                         

 
<210> 197
<211> 879
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Imine reductase variant from Pseudonocardia spinosispora
 
<400> 197
atgagtgatt cacctgtgcc cgtgacagtt ctgggtcttg gtctgatggg tcaggcctta 60
gcgggagcgt ttctggcagc cggtcaccct acgacggttt ggaatcgcag cgcagctaaa 120
gctgatgaac tggttggtcg cggtgcaacc ctggccgcgt ctcccgcggc tgccgtcgca 180
gcgggtagtc tgatcgttgt ttgcgtcaca gatcatcgtg cggtgcgcga actgctggaa 240
ccacttggcg atgcactgcc aggtcgcgtt ctggtcaacc tgacgagcgg tacgtcgcgt 300
gaagcccgtg agaacgcaga acatactgcg gctcagggag ccacctacct ggatggtgtg 360
attctgacca ttccaagcgg cattggcacc ccagaagcta ccattctgta ttcgggaccg 420
cgcagtacct tcgatgaaca tgaaagcgca ctgcgcgcat taggtccggg ggcgaccttt 480
gtaggggaag atcacggact ggctgccctg tatgatgcag caatgtcgag cgtgatgtgg 540
ggcctgctga atggtttcct gcatggaacc gcactgctgg gaaccgcggg cgtttcggca 600
acctctgtgg ttccgatgct gggtcaaggt atagaagcga tgactggtct aattgcgcgc 660
tacgcagatc agattgatgc cggtgtctat ccagcagacg atgccacgat tgatacccag 720
cgtgctgcaa tgcagcacct ggtcgaggag agccgtgcgg cgggcatcaa tggcgaactt 780
ccggcgtttg tgaccaaact ggccgatcgc gcagtggcag aaggtcgtgg tggtgactct 840
tacgctgcac tgattgaaca gtttcgcaag tcagcttaa                        879

 
<210> 198
<211> 292
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Imine reductase variant from Pseudonocardia spinosispora
 
<400> 198
Met Ser Asp Ser Pro Val Pro Val Thr Val Leu Gly Leu Gly Leu Met 
1               5                   10                  15      
Gly Gln Ala Leu Ala Gly Ala Phe Leu Ala Ala Gly His Pro Thr Thr 
            20                  25                  30          
Val Trp Asn Arg Ser Ala Ala Lys Ala Asp Glu Leu Val Gly Arg Gly 
        35                  40                  45              
Ala Thr Leu Ala Ala Ser Pro Ala Ala Ala Val Ala Ala Gly Ser Leu 
    50                  55                  60                  
Ile Val Val Cys Val Thr Asp His Arg Ala Val Arg Glu Leu Leu Glu 
65                  70                  75                  80  
Pro Leu Gly Asp Ala Leu Pro Gly Arg Val Leu Val Asn Leu Thr Ser 
                85                  90                  95      
Gly Thr Ser Arg Glu Ala Arg Glu Asn Ala Glu His Thr Ala Ala Gln 
            100                 105                 110         
Gly Ala Thr Tyr Leu Asp Gly Val Ile Leu Thr Ile Pro Ser Gly Ile 
        115                 120                 125             
Gly Thr Pro Glu Ala Thr Ile Leu Tyr Ser Gly Pro Arg Ser Thr Phe 
    130                 135                 140                 
Asp Glu His Glu Ser Ala Leu Arg Ala Leu Gly Pro Gly Ala Thr Phe 
145                 150                 155                 160 
Val Gly Glu Asp His Gly Leu Ala Ala Leu Tyr Asp Ala Ala Met Ser 
                165                 170                 175     
Ser Val Met Trp Gly Leu Leu Asn Gly Phe Leu His Gly Thr Ala Leu 
            180                 185                 190         
Leu Gly Thr Ala Gly Val Ser Ala Thr Ser Val Val Pro Met Leu Gly 
        195                 200                 205             
Gln Gly Ile Glu Ala Met Thr Gly Leu Ile Ala Arg Tyr Ala Asp Gln 
    210                 215                 220                 
Ile Asp Ala Gly Val Tyr Pro Ala Asp Asp Ala Thr Ile Asp Thr Gln 
225                 230                 235                 240 
Arg Ala Ala Met Gln His Leu Val Glu Glu Ser Arg Ala Ala Gly Ile 
                245                 250                 255     
Asn Gly Glu Leu Pro Ala Phe Val Thr Lys Leu Ala Asp Arg Ala Val 
            260                 265                 270         
Ala Glu Gly Arg Gly Gly Asp Ser Tyr Ala Ala Leu Ile Glu Gln Phe 
        275                 280                 285             
Arg Lys Ser Ala                                                 
    290                                                         

 
<210> 199
<211> 879
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Imine reductase variant from Pseudonocardia spinosispora
 
<400> 199
atgagtgatt cacctgtgcc cgtgacagtt ctgggtcttg gtctgatggg tcaggcctta 60
gcgggagcgt ttctggcagc cggtcaccct acgacggttt ggaatcgcag cgcagctaaa 120
gctgatgaac tggttggtcg cggtgcaacc ctggccgcgt ctcccgcggc tgccgtcgca 180
gcgggtagtc tgatcgttgt ttgcgtcaca gatcatcgtg cggtgcgcga actgctggaa 240
ccacttggcg atgcactgcc aggtcgcgtt ctggtcaacc tgacgagcgg tacgtcgcgt 300
gaagcccgtg agaacgcaga acatactgcg gctcagggag ccacctacct ggatggtgtg 360
attctgacca ttccaagcgg cattggcacc ccagaagcta cgattctgta ttcgggaccg 420
cgcagtacct tcgatgaaca tgaaagcgca ctgcgcgcat taggtccggg ggcgaccttt 480
gtaggggaag atcacggact ggctgccctg tatgatgcag caatgtcgag cattatgtgg 540
ggcctgctga atggtttcct gcatggaacc gcactgctgg gaaccgcggg cgtttcggca 600
acctctgtgg ttccgatgct gggtcaaggt tgggaagcgg tgttgggtct aattgcgcgc 660
tacgcagatc agattgatgc cggtgtctat ccagcagacg atgccacgat tgatacccag 720
cgtgctgcaa tgcagcacct ggtcgaggag agccgtgcgg cgggcatcaa tggcgaactt 780
ccggcgtttg tgaccaaact ggccgatcgc gcagtggcag aaggtcgtgg tggtgactct 840
tacgctgcac tgattgaaca gtttcgcaag tcagcttaa                        879

 
<210> 200
<211> 292
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Imine reductase variant from Pseudonocardia spinosispora
 
<400> 200
Met Ser Asp Ser Pro Val Pro Val Thr Val Leu Gly Leu Gly Leu Met 
1               5                   10                  15      
Gly Gln Ala Leu Ala Gly Ala Phe Leu Ala Ala Gly His Pro Thr Thr 
            20                  25                  30          
Val Trp Asn Arg Ser Ala Ala Lys Ala Asp Glu Leu Val Gly Arg Gly 
        35                  40                  45              
Ala Thr Leu Ala Ala Ser Pro Ala Ala Ala Val Ala Ala Gly Ser Leu 
    50                  55                  60                  
Ile Val Val Cys Val Thr Asp His Arg Ala Val Arg Glu Leu Leu Glu 
65                  70                  75                  80  
Pro Leu Gly Asp Ala Leu Pro Gly Arg Val Leu Val Asn Leu Thr Ser 
                85                  90                  95      
Gly Thr Ser Arg Glu Ala Arg Glu Asn Ala Glu His Thr Ala Ala Gln 
            100                 105                 110         
Gly Ala Thr Tyr Leu Asp Gly Val Ile Leu Thr Ile Pro Ser Gly Ile 
        115                 120                 125             
Gly Thr Pro Glu Ala Thr Ile Leu Tyr Ser Gly Pro Arg Ser Thr Phe 
    130                 135                 140                 
Asp Glu His Glu Ser Ala Leu Arg Ala Leu Gly Pro Gly Ala Thr Phe 
145                 150                 155                 160 
Val Gly Glu Asp His Gly Leu Ala Ala Leu Tyr Asp Ala Ala Met Ser 
                165                 170                 175     
Ser Ile Met Trp Gly Leu Leu Asn Gly Phe Leu His Gly Thr Ala Leu 
            180                 185                 190         
Leu Gly Thr Ala Gly Val Ser Ala Thr Ser Val Val Pro Met Leu Gly 
        195                 200                 205             
Gln Gly Trp Glu Ala Val Leu Gly Leu Ile Ala Arg Tyr Ala Asp Gln 
    210                 215                 220                 
Ile Asp Ala Gly Val Tyr Pro Ala Asp Asp Ala Thr Ile Asp Thr Gln 
225                 230                 235                 240 
Arg Ala Ala Met Gln His Leu Val Glu Glu Ser Arg Ala Ala Gly Ile 
                245                 250                 255     
Asn Gly Glu Leu Pro Ala Phe Val Thr Lys Leu Ala Asp Arg Ala Val 
            260                 265                 270         
Ala Glu Gly Arg Gly Gly Asp Ser Tyr Ala Ala Leu Ile Glu Gln Phe 
        275                 280                 285             
Arg Lys Ser Ala                                                 
    290                                                         

 
<210> 201
<211> 879
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Imine reductase variant from Pseudonocardia spinosispora
 
<400> 201
atgagtgatt cacctgtgcc cgtgacagtt ctgggtcttg gtctgatggg tcaggcctta 60
gcgggagcgt ttctggcagc cggtcaccct acgacggttt ggaatcgcag cgcagctaaa 120
gctgatgaac tggttggtcg cggtgcaacc ctggccgcgt ctcccgcggc tgccgtcgca 180
gcgggtagtc tgatcgttgt ttgcgtcaca gatcatcgtg cggtgcgcga actgctggaa 240
ccacttggcg atgcactgcc aggtcgcgtt ctggtcaacc tgacgagcgg tacgtcgcgt 300
gaagcccgtg agaacgcaga acatactgcg gctcagggag ccacctacct ggatggtgtg 360
attctgacca ttccaagcgg cattggcacc ccagaagcta cgattctgta ttcgggaccg 420
cgcagtacct tcgatgaaca tgaaagcgca ctgcgcgcat taggtccggg ggcgaccttt 480
gtaggggaag atcacggact ggctgccctg tatgatgcag caatgtcgag cattatgtgg 540
ggcctgctga atggtttcct gcatggaacc gcactgctgg gaaccgcggg cgtttcggca 600
acctctgtgg ttccgatgct gggtcaaggt tgggaagcgg tgactggtat aattgcgcgc 660
tacgcagatc agattgatgc cggtgtctat ccagcagacg atgccacgat tgatacccag 720
cgtgctgcaa tgcagcacct ggtcgaggag agccgtgcgg cgggcatcaa tggcgaactt 780
ccggcgtttg tgaccaaact ggccgatcgc gcagtggcag aaggtcgtgg tggtgactct 840
tacgctgcac tgattgaaca gtttcgcaag tcagcttaa                        879

 
<210> 202
<211> 292
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Imine reductase variant from Pseudonocardia spinosispora
 
<400> 202
Met Ser Asp Ser Pro Val Pro Val Thr Val Leu Gly Leu Gly Leu Met 
1               5                   10                  15      
Gly Gln Ala Leu Ala Gly Ala Phe Leu Ala Ala Gly His Pro Thr Thr 
            20                  25                  30          
Val Trp Asn Arg Ser Ala Ala Lys Ala Asp Glu Leu Val Gly Arg Gly 
        35                  40                  45              
Ala Thr Leu Ala Ala Ser Pro Ala Ala Ala Val Ala Ala Gly Ser Leu 
    50                  55                  60                  
Ile Val Val Cys Val Thr Asp His Arg Ala Val Arg Glu Leu Leu Glu 
65                  70                  75                  80  
Pro Leu Gly Asp Ala Leu Pro Gly Arg Val Leu Val Asn Leu Thr Ser 
                85                  90                  95      
Gly Thr Ser Arg Glu Ala Arg Glu Asn Ala Glu His Thr Ala Ala Gln 
            100                 105                 110         
Gly Ala Thr Tyr Leu Asp Gly Val Ile Leu Thr Ile Pro Ser Gly Ile 
        115                 120                 125             
Gly Thr Pro Glu Ala Thr Ile Leu Tyr Ser Gly Pro Arg Ser Thr Phe 
    130                 135                 140                 
Asp Glu His Glu Ser Ala Leu Arg Ala Leu Gly Pro Gly Ala Thr Phe 
145                 150                 155                 160 
Val Gly Glu Asp His Gly Leu Ala Ala Leu Tyr Asp Ala Ala Met Ser 
                165                 170                 175     
Ser Ile Met Trp Gly Leu Leu Asn Gly Phe Leu His Gly Thr Ala Leu 
            180                 185                 190         
Leu Gly Thr Ala Gly Val Ser Ala Thr Ser Val Val Pro Met Leu Gly 
        195                 200                 205             
Gln Gly Trp Glu Ala Val Thr Gly Ile Ile Ala Arg Tyr Ala Asp Gln 
    210                 215                 220                 
Ile Asp Ala Gly Val Tyr Pro Ala Asp Asp Ala Thr Ile Asp Thr Gln 
225                 230                 235                 240 
Arg Ala Ala Met Gln His Leu Val Glu Glu Ser Arg Ala Ala Gly Ile 
                245                 250                 255     
Asn Gly Glu Leu Pro Ala Phe Val Thr Lys Leu Ala Asp Arg Ala Val 
            260                 265                 270         
Ala Glu Gly Arg Gly Gly Asp Ser Tyr Ala Ala Leu Ile Glu Gln Phe 
        275                 280                 285             
Arg Lys Ser Ala                                                 
    290                                                         

 
<210> 203
<211> 879
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Imine reductase variant from Pseudonocardia spinosispora
 
<400> 203
atgagtgatt cacctgtgcc cgtgacagtt ctgggtcttg gtctgatggg tcaggcctta 60
gcgggagcgt ttctggcagc cggtcaccct acgacggttt ggaatcgcag cgcagctaaa 120
gctgatgaac tggttggtcg cggtgcaacc ctggccgcgt ctcccgcggc tgccgtcgca 180
gcgggtagtc tgatcgttgt ttgcgtcaca gatcatcgtg cggtgcgcga actgctggaa 240
ccacttggcg atgcactgcc aggtcgcgtt ctggtcaacc tgacgagcgg tacgtcgcgt 300
gaagcccgtg agaacgcaga acatactgcg gctcagggag ccacctacct ggatggtgtg 360
attctgacca ttccaagcgg cattggcacc ccagaagcta cgattctgta ttcgggaccg 420
cgcagtacct tcgatgaaca tgaaagcgca ctgcgcgcat taggtccggg ggcgaccttt 480
gtaggggaag atcacggact ggctaacctg tatgatgcag caatgtcgag catgatgtgg 540
ggcctgctga atggtttcct gcatggaacc gcactgctgg gaaccgcggg cgtttcggca 600
acctctgtgg ttccgatgct gggtcaaggt atcgaagcgg tgaccggttg gattgcgcgc 660
tacgcagatc agattgatgc cggtgtctat ccagcagacg atgccacgat tgatacccag 720
cgtgctgcaa tgcagcacct ggtcgaggag agccgtgcgg cgggcatcaa tggcgaactt 780
ccggcgtttg tgaccaaact ggccgatcgc gcagtggcag aaggtcgtgg tggtgactct 840
tacgctgcac tgattgaaca gtttcgcaag tcagcttaa                        879

 
<210> 204
<211> 292
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Imine reductase variant from Pseudonocardia spinosispora
 
<400> 204
Met Ser Asp Ser Pro Val Pro Val Thr Val Leu Gly Leu Gly Leu Met 
1               5                   10                  15      
Gly Gln Ala Leu Ala Gly Ala Phe Leu Ala Ala Gly His Pro Thr Thr 
            20                  25                  30          
Val Trp Asn Arg Ser Ala Ala Lys Ala Asp Glu Leu Val Gly Arg Gly 
        35                  40                  45              
Ala Thr Leu Ala Ala Ser Pro Ala Ala Ala Val Ala Ala Gly Ser Leu 
    50                  55                  60                  
Ile Val Val Cys Val Thr Asp His Arg Ala Val Arg Glu Leu Leu Glu 
65                  70                  75                  80  
Pro Leu Gly Asp Ala Leu Pro Gly Arg Val Leu Val Asn Leu Thr Ser 
                85                  90                  95      
Gly Thr Ser Arg Glu Ala Arg Glu Asn Ala Glu His Thr Ala Ala Gln 
            100                 105                 110         
Gly Ala Thr Tyr Leu Asp Gly Val Ile Leu Thr Ile Pro Ser Gly Ile 
        115                 120                 125             
Gly Thr Pro Glu Ala Thr Ile Leu Tyr Ser Gly Pro Arg Ser Thr Phe 
    130                 135                 140                 
Asp Glu His Glu Ser Ala Leu Arg Ala Leu Gly Pro Gly Ala Thr Phe 
145                 150                 155                 160 
Val Gly Glu Asp His Gly Leu Ala Asn Leu Tyr Asp Ala Ala Met Ser 
                165                 170                 175     
Ser Met Met Trp Gly Leu Leu Asn Gly Phe Leu His Gly Thr Ala Leu 
            180                 185                 190         
Leu Gly Thr Ala Gly Val Ser Ala Thr Ser Val Val Pro Met Leu Gly 
        195                 200                 205             
Gln Gly Ile Glu Ala Val Thr Gly Trp Ile Ala Arg Tyr Ala Asp Gln 
    210                 215                 220                 
Ile Asp Ala Gly Val Tyr Pro Ala Asp Asp Ala Thr Ile Asp Thr Gln 
225                 230                 235                 240 
Arg Ala Ala Met Gln His Leu Val Glu Glu Ser Arg Ala Ala Gly Ile 
                245                 250                 255     
Asn Gly Glu Leu Pro Ala Phe Val Thr Lys Leu Ala Asp Arg Ala Val 
            260                 265                 270         
Ala Glu Gly Arg Gly Gly Asp Ser Tyr Ala Ala Leu Ile Glu Gln Phe 
        275                 280                 285             
Arg Lys Ser Ala                                                 
    290                                                         

 
<210> 205
<211> 879
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Imine reductase variant from Pseudonocardia spinosispora
 
<400> 205
atgagtgatt cacctgtgcc cgtgacagtt ctgggtcttg gtctgatggg tcaggcctta 60
gcgggagcgt ttctggcagc cggtcaccct acgacggttt ggaatcgcag cgcagctaaa 120
gctgatgaac tggttggtcg cggtgcaacc ctggccgcgt ctcccgcggc tgccgtcgca 180
gcgggtagtc tgatcgttgt ttgcgtcaca gatcatcgtg cggtgcgcga actgctggaa 240
ccacttggcg atgcactgcc aggtcgcgtt ctggtcaacc tgacgagcgg tacgtcgcgt 300
gaagcccgtg agaacgcaga acatactgcg gctcagggag ccacctacct ggatggtgtg 360
attctgacca ttccaagcgg cattggcacc ccagaagcta cgattctgta ttcgggaccg 420
cgcagtacct tcgatgaaca tgaaagcgca ctgcgcgcat taggtccggg ggcgaccttt 480
gtaggggaag atcacggact ggctaacctg tatgatgcag caatgttgag cattatgtgg 540
ggcctgctga atggtttcct gcatggaacc gcactgctgg gaaccgcggg cgtttcggca 600
acctctgtgg ttccgatgct gggtcaaggt atcgaagcgg tgactggtat aattgcgcgc 660
tacgcagatc agattgatgc cggtgtctat ccagcagacg acgccacgat tgatacccag 720
cgtgctgcaa tgcagcacct ggtcgaggag agccgtgcgg cgggcatcaa tggcgaactt 780
ccggcgtttg tgaccaaact ggccgatcgc gcagtggcag aaggtcgtgg tggtgactct 840
tacgctgcac tgattgaaca gtttcgcaag tcagcttaa                        879

 
<210> 206
<211> 292
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Imine reductase variant from Pseudonocardia spinosispora
 
<400> 206
Met Ser Asp Ser Pro Val Pro Val Thr Val Leu Gly Leu Gly Leu Met 
1               5                   10                  15      
Gly Gln Ala Leu Ala Gly Ala Phe Leu Ala Ala Gly His Pro Thr Thr 
            20                  25                  30          
Val Trp Asn Arg Ser Ala Ala Lys Ala Asp Glu Leu Val Gly Arg Gly 
        35                  40                  45              
Ala Thr Leu Ala Ala Ser Pro Ala Ala Ala Val Ala Ala Gly Ser Leu 
    50                  55                  60                  
Ile Val Val Cys Val Thr Asp His Arg Ala Val Arg Glu Leu Leu Glu 
65                  70                  75                  80  
Pro Leu Gly Asp Ala Leu Pro Gly Arg Val Leu Val Asn Leu Thr Ser 
                85                  90                  95      
Gly Thr Ser Arg Glu Ala Arg Glu Asn Ala Glu His Thr Ala Ala Gln 
            100                 105                 110         
Gly Ala Thr Tyr Leu Asp Gly Val Ile Leu Thr Ile Pro Ser Gly Ile 
        115                 120                 125             
Gly Thr Pro Glu Ala Thr Ile Leu Tyr Ser Gly Pro Arg Ser Thr Phe 
    130                 135                 140                 
Asp Glu His Glu Ser Ala Leu Arg Ala Leu Gly Pro Gly Ala Thr Phe 
145                 150                 155                 160 
Val Gly Glu Asp His Gly Leu Ala Asn Leu Tyr Asp Ala Ala Met Leu 
                165                 170                 175     
Ser Ile Met Trp Gly Leu Leu Asn Gly Phe Leu His Gly Thr Ala Leu 
            180                 185                 190         
Leu Gly Thr Ala Gly Val Ser Ala Thr Ser Val Val Pro Met Leu Gly 
        195                 200                 205             
Gln Gly Ile Glu Ala Val Thr Gly Ile Ile Ala Arg Tyr Ala Asp Gln 
    210                 215                 220                 
Ile Asp Ala Gly Val Tyr Pro Ala Asp Asp Ala Thr Ile Asp Thr Gln 
225                 230                 235                 240 
Arg Ala Ala Met Gln His Leu Val Glu Glu Ser Arg Ala Ala Gly Ile 
                245                 250                 255     
Asn Gly Glu Leu Pro Ala Phe Val Thr Lys Leu Ala Asp Arg Ala Val 
            260                 265                 270         
Ala Glu Gly Arg Gly Gly Asp Ser Tyr Ala Ala Leu Ile Glu Gln Phe 
        275                 280                 285             
Arg Lys Ser Ala                                                 
    290                                                         

 
<210> 207
<211> 879
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Imine reductase variant from Pseudonocardia spinosispora
 
<400> 207
atgagtgatt cacctgtgcc cgtgacagtt ctgggtcttg gtctgatggg tcaggcctta 60
gcgggagcgt ttctggcagc cggtcaccct acgacggttt ggaatcgcag cgcagctaaa 120
gctgatgaac tggttggtcg cggtgcaacc ctggccgcgt ctcccgcggc tgccgtcgca 180
gcgggtagtc tgatcgttgt ttgcgtcaca gatcatcgtg cggtgcgcga actgctggaa 240
ccacttggcg atgcactgcc aggtcgcgtt ctggtcaacc tgacgagcgg tacgtcgcgt 300
gaagcccgtg agaacgcaga acatactgcg gctcagggag ccacctacct ggatggtgtg 360
attctgacca ttccaagcgg cattggcacc ccagaagcta ggattctgta ttcgggaccg 420
cgcagtacct tcgatgaaca tgaaagcgca ctgcgcgcat taggtccggg ggcgaccttt 480
gtaggggaag atcacggact ggctaacctg tatgatgcag caatgtcgag cattatgtgg 540
ggcctgctga atggtttcct gcatggaacc gcactgctgg gaaccgcggg cgtttcggca 600
acctctgtgg ttccgatgct gggtcaaggt tgggaagcgg tgactggttg gattgcgcgc 660
tacgcagatc agattgatgc cggtgtctat ccagcagacg atgccacgat tgatacccag 720
cgtgctgcaa tgcagcacct ggtcgaggag agccgtgcgg cgggcatcaa tggcgaactt 780
ccggcgtttg tgaccaaact ggccgatcgc gcagtggcag aaggtcgtgg tggtgactct 840
tacgctgcac tgattgaaca gtttcgcaag tcagcttaa                        879

 
<210> 208
<211> 292
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Imine reductase variant from Pseudonocardia spinosispora
 
<400> 208
Met Ser Asp Ser Pro Val Pro Val Thr Val Leu Gly Leu Gly Leu Met 
1               5                   10                  15      
Gly Gln Ala Leu Ala Gly Ala Phe Leu Ala Ala Gly His Pro Thr Thr 
            20                  25                  30          
Val Trp Asn Arg Ser Ala Ala Lys Ala Asp Glu Leu Val Gly Arg Gly 
        35                  40                  45              
Ala Thr Leu Ala Ala Ser Pro Ala Ala Ala Val Ala Ala Gly Ser Leu 
    50                  55                  60                  
Ile Val Val Cys Val Thr Asp His Arg Ala Val Arg Glu Leu Leu Glu 
65                  70                  75                  80  
Pro Leu Gly Asp Ala Leu Pro Gly Arg Val Leu Val Asn Leu Thr Ser 
                85                  90                  95      
Gly Thr Ser Arg Glu Ala Arg Glu Asn Ala Glu His Thr Ala Ala Gln 
            100                 105                 110         
Gly Ala Thr Tyr Leu Asp Gly Val Ile Leu Thr Ile Pro Ser Gly Ile 
        115                 120                 125             
Gly Thr Pro Glu Ala Arg Ile Leu Tyr Ser Gly Pro Arg Ser Thr Phe 
    130                 135                 140                 
Asp Glu His Glu Ser Ala Leu Arg Ala Leu Gly Pro Gly Ala Thr Phe 
145                 150                 155                 160 
Val Gly Glu Asp His Gly Leu Ala Asn Leu Tyr Asp Ala Ala Met Ser 
                165                 170                 175     
Ser Ile Met Trp Gly Leu Leu Asn Gly Phe Leu His Gly Thr Ala Leu 
            180                 185                 190         
Leu Gly Thr Ala Gly Val Ser Ala Thr Ser Val Val Pro Met Leu Gly 
        195                 200                 205             
Gln Gly Trp Glu Ala Val Thr Gly Trp Ile Ala Arg Tyr Ala Asp Gln 
    210                 215                 220                 
Ile Asp Ala Gly Val Tyr Pro Ala Asp Asp Ala Thr Ile Asp Thr Gln 
225                 230                 235                 240 
Arg Ala Ala Met Gln His Leu Val Glu Glu Ser Arg Ala Ala Gly Ile 
                245                 250                 255     
Asn Gly Glu Leu Pro Ala Phe Val Thr Lys Leu Ala Asp Arg Ala Val 
            260                 265                 270         
Ala Glu Gly Arg Gly Gly Asp Ser Tyr Ala Ala Leu Ile Glu Gln Phe 
        275                 280                 285             
Arg Lys Ser Ala                                                 
    290                                                         

 
<210> 209
<211> 879
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Imine reductase variant from Pseudonocardia spinosispora
 
<400> 209
atgagtgatt cacctgtgcc cgtgacagtt ctgggtcttg gtctgatggg tcaggcctta 60
gcgggagcgt ttctggcagc cggtcaccct acgacggttt ggaatcgcag cgcagctaaa 120
gctgatgaac tggttggtcg cggtgcaacc ctggccgcgt ctcccgcggc tgccgtcgca 180
gcgggtagtc tgatcgttgt ttgcgtcaca gatcatcgtg cggtgcgcga actgctggaa 240
ccacttggcg atgcactgcc aggtcgcgtt ctggtcaacc tgacgagcgg tacgtcgcgt 300
gaagcccgtg agaacgcaga acatactgcg gctcagggag ccacctacct ggatggtgtg 360
attctgacca ttccaagcgg cattggcacc ccagaagcta ccattctgta ttcgggaccg 420
cgcagtacct tcgatgaaca tgaaagcgca ctgcgcgcat taggtccggg ggcgaccttt 480
gtaggggaag atcacggact ggctaacctg tatgatgcag caatgttgag cgtgatgtgg 540
ggcctgctga atggtttcct gcatggaacc gcactgctgg gaaccgcggg cgtttcggca 600
acctctgtgg ttccgatgct gggtcaaggt tgggaagcga tgttgggttg gattgcgcgc 660
tacgcagatc agattgatgc cggtgtctat ccagcagacg acgccacgat tgatacccag 720
cgtgctgcaa tgcagcacct ggtcgaggag agccgtgcgg cgggcatcaa tggcgaactt 780
ccggcgtttg tgaccaaact ggccgatcgc gcagtggcag aaggtcgtgg tggtgactct 840
tacgctgcac tgattgaaca gtttcgcaag tcagcttaa                        879

 
<210> 210
<211> 292
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Imine reductase variant from Pseudonocardia spinosispora
 
<400> 210
Met Ser Asp Ser Pro Val Pro Val Thr Val Leu Gly Leu Gly Leu Met 
1               5                   10                  15      
Gly Gln Ala Leu Ala Gly Ala Phe Leu Ala Ala Gly His Pro Thr Thr 
            20                  25                  30          
Val Trp Asn Arg Ser Ala Ala Lys Ala Asp Glu Leu Val Gly Arg Gly 
        35                  40                  45              
Ala Thr Leu Ala Ala Ser Pro Ala Ala Ala Val Ala Ala Gly Ser Leu 
    50                  55                  60                  
Ile Val Val Cys Val Thr Asp His Arg Ala Val Arg Glu Leu Leu Glu 
65                  70                  75                  80  
Pro Leu Gly Asp Ala Leu Pro Gly Arg Val Leu Val Asn Leu Thr Ser 
                85                  90                  95      
Gly Thr Ser Arg Glu Ala Arg Glu Asn Ala Glu His Thr Ala Ala Gln 
            100                 105                 110         
Gly Ala Thr Tyr Leu Asp Gly Val Ile Leu Thr Ile Pro Ser Gly Ile 
        115                 120                 125             
Gly Thr Pro Glu Ala Thr Ile Leu Tyr Ser Gly Pro Arg Ser Thr Phe 
    130                 135                 140                 
Asp Glu His Glu Ser Ala Leu Arg Ala Leu Gly Pro Gly Ala Thr Phe 
145                 150                 155                 160 
Val Gly Glu Asp His Gly Leu Ala Asn Leu Tyr Asp Ala Ala Met Leu 
                165                 170                 175     
Ser Val Met Trp Gly Leu Leu Asn Gly Phe Leu His Gly Thr Ala Leu 
            180                 185                 190         
Leu Gly Thr Ala Gly Val Ser Ala Thr Ser Val Val Pro Met Leu Gly 
        195                 200                 205             
Gln Gly Trp Glu Ala Met Leu Gly Trp Ile Ala Arg Tyr Ala Asp Gln 
    210                 215                 220                 
Ile Asp Ala Gly Val Tyr Pro Ala Asp Asp Ala Thr Ile Asp Thr Gln 
225                 230                 235                 240 
Arg Ala Ala Met Gln His Leu Val Glu Glu Ser Arg Ala Ala Gly Ile 
                245                 250                 255     
Asn Gly Glu Leu Pro Ala Phe Val Thr Lys Leu Ala Asp Arg Ala Val 
            260                 265                 270         
Ala Glu Gly Arg Gly Gly Asp Ser Tyr Ala Ala Leu Ile Glu Gln Phe 
        275                 280                 285             
Arg Lys Ser Ala                                                 
    290                                                         

 
<210> 211
<211> 879
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Imine reductase variant from Pseudonocardia spinosispora
 
<400> 211
atgagtgatt cacctgtgcc cgtgacagtt ctgggtcttg gtctgatggg tcaggcctta 60
gcgggagcgt ttctggcagc cggtcaccct acgacggttt ggaatcgcag cgcagctaaa 120
gctgatgaac tggttggtcg cggtgcaacc ctggccgcgt ctcccgcggc tgccgtcgca 180
gcgggtagtc tgatcgttgt ttgcgtcaca gatcatcgtg cggtgcgcga actgctggaa 240
ccacttggcg atgcactgcc aggtcgcgtt ctggtcaacc tgacgagcgg tacgtcgcgt 300
gaagcccgtg agaacgcaga acatactgcg gctcagggag ccacctacct ggatggtgtg 360
attctgacca ttccaagcgg cattggcacc ccagaagcta ccattctgta ttcgggaccg 420
cgcagtacct tcgatgaaca tgaaagcgca ctgcgcgcat taggtccggg ggcgaccttt 480
gtaggggaag atcacggact ggctgccctg tatgatgcag caatgttgag cattatgtgg 540
ggcctgctga atggtttcct gcatggaacc gcactgctgg gaaccgcggg cgtttcggca 600
acctctgtgg ttccgatgct gggtcaaggt atcgaagcgg tgatgggttg gattgcgcgc 660
tacgcagatc agattgatgc cggtgtctat ccagcagacg atgccacgat tgatacccag 720
cgtgctgcaa tgcagcacct ggtcgaggag agccgtgcgg cgggcatcaa tggcgaactt 780
ccggcgtttg tgaccaaact ggccgatcgc gcagtggcag aaggtcgtgg tggtgactct 840
tacgctgcac tgattgaaca gtttcgcaag tcagcttaa                        879

 
<210> 212
<211> 292
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Imine reductase variant from Pseudonocardia spinosispora
 
<400> 212
Met Ser Asp Ser Pro Val Pro Val Thr Val Leu Gly Leu Gly Leu Met 
1               5                   10                  15      
Gly Gln Ala Leu Ala Gly Ala Phe Leu Ala Ala Gly His Pro Thr Thr 
            20                  25                  30          
Val Trp Asn Arg Ser Ala Ala Lys Ala Asp Glu Leu Val Gly Arg Gly 
        35                  40                  45              
Ala Thr Leu Ala Ala Ser Pro Ala Ala Ala Val Ala Ala Gly Ser Leu 
    50                  55                  60                  
Ile Val Val Cys Val Thr Asp His Arg Ala Val Arg Glu Leu Leu Glu 
65                  70                  75                  80  
Pro Leu Gly Asp Ala Leu Pro Gly Arg Val Leu Val Asn Leu Thr Ser 
                85                  90                  95      
Gly Thr Ser Arg Glu Ala Arg Glu Asn Ala Glu His Thr Ala Ala Gln 
            100                 105                 110         
Gly Ala Thr Tyr Leu Asp Gly Val Ile Leu Thr Ile Pro Ser Gly Ile 
        115                 120                 125             
Gly Thr Pro Glu Ala Thr Ile Leu Tyr Ser Gly Pro Arg Ser Thr Phe 
    130                 135                 140                 
Asp Glu His Glu Ser Ala Leu Arg Ala Leu Gly Pro Gly Ala Thr Phe 
145                 150                 155                 160 
Val Gly Glu Asp His Gly Leu Ala Ala Leu Tyr Asp Ala Ala Met Leu 
                165                 170                 175     
Ser Ile Met Trp Gly Leu Leu Asn Gly Phe Leu His Gly Thr Ala Leu 
            180                 185                 190         
Leu Gly Thr Ala Gly Val Ser Ala Thr Ser Val Val Pro Met Leu Gly 
        195                 200                 205             
Gln Gly Ile Glu Ala Val Met Gly Trp Ile Ala Arg Tyr Ala Asp Gln 
    210                 215                 220                 
Ile Asp Ala Gly Val Tyr Pro Ala Asp Asp Ala Thr Ile Asp Thr Gln 
225                 230                 235                 240 
Arg Ala Ala Met Gln His Leu Val Glu Glu Ser Arg Ala Ala Gly Ile 
                245                 250                 255     
Asn Gly Glu Leu Pro Ala Phe Val Thr Lys Leu Ala Asp Arg Ala Val 
            260                 265                 270         
Ala Glu Gly Arg Gly Gly Asp Ser Tyr Ala Ala Leu Ile Glu Gln Phe 
        275                 280                 285             
Arg Lys Ser Ala                                                 
    290                                                         

 
<210> 213
<211> 879
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Imine reductase variant from Pseudonocardia spinosispora
 
<400> 213
atgagtgatt cacctgtgcc cgtgacagtt ctgggtcttg gtctgatggg tcaggcctta 60
gcgggagcgt ttctggcagc cggtcaccct acgacggttt ggaatcgcag cgcagctaaa 120
gctgatgaac tggttggtcg cggtgcaacc ctggccgcgt ctcccgcggc tgccgtcgca 180
gcgggtagtc tgatcgttgt ttgcgtcaca gatcatcgtg cggtgcgcga actgctggaa 240
ccacttggcg atgcactgcc aggtcgcgtt ctggtcaacc tgacgagcgg tacgtcgcgt 300
gaagcccgtg agaacgcaga acatactgcg gctcagggag ccacctacct ggatggtgtg 360
attctgacca ttccaagcgg cattggcacc ccagaagcta ggattctgta ttcgggaccg 420
cgcagtacct tcgatgaaca tgaaagcgca ctgcgcgcat taggtccggg ggcgaccttt 480
gtaggggaag atcacggact ggctaacctg tatgatgcag caatgtcgag cgtgatgtgg 540
ggcctgctga atggtttcct gcatggaacc gcactgctgg gaaccgcggg cgtttcggca 600
acctctgtgg ttccgatgct gggtcaaggt ctagaagcgg tgatgggtat aattgcgcgc 660
tacgcagatc agattgatgc cggtgtctat ccagcagacc tcgccacgat tgatacccag 720
cgtgctgcaa tgcagcacct ggtcgaggag agccgtgcgg cgggcatcaa tggcgaactt 780
ccggcgtttg tgaccaaact ggccgatcgc gcagtggcag aaggtcgtgg tggtgactct 840
tacgctgcac tgattgaaca gtttcgcaag tcagcttaa                        879

 
<210> 214
<211> 292
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Imine reductase variant from Pseudonocardia spinosispora
 
<400> 214
Met Ser Asp Ser Pro Val Pro Val Thr Val Leu Gly Leu Gly Leu Met 
1               5                   10                  15      
Gly Gln Ala Leu Ala Gly Ala Phe Leu Ala Ala Gly His Pro Thr Thr 
            20                  25                  30          
Val Trp Asn Arg Ser Ala Ala Lys Ala Asp Glu Leu Val Gly Arg Gly 
        35                  40                  45              
Ala Thr Leu Ala Ala Ser Pro Ala Ala Ala Val Ala Ala Gly Ser Leu 
    50                  55                  60                  
Ile Val Val Cys Val Thr Asp His Arg Ala Val Arg Glu Leu Leu Glu 
65                  70                  75                  80  
Pro Leu Gly Asp Ala Leu Pro Gly Arg Val Leu Val Asn Leu Thr Ser 
                85                  90                  95      
Gly Thr Ser Arg Glu Ala Arg Glu Asn Ala Glu His Thr Ala Ala Gln 
            100                 105                 110         
Gly Ala Thr Tyr Leu Asp Gly Val Ile Leu Thr Ile Pro Ser Gly Ile 
        115                 120                 125             
Gly Thr Pro Glu Ala Arg Ile Leu Tyr Ser Gly Pro Arg Ser Thr Phe 
    130                 135                 140                 
Asp Glu His Glu Ser Ala Leu Arg Ala Leu Gly Pro Gly Ala Thr Phe 
145                 150                 155                 160 
Val Gly Glu Asp His Gly Leu Ala Asn Leu Tyr Asp Ala Ala Met Ser 
                165                 170                 175     
Ser Val Met Trp Gly Leu Leu Asn Gly Phe Leu His Gly Thr Ala Leu 
            180                 185                 190         
Leu Gly Thr Ala Gly Val Ser Ala Thr Ser Val Val Pro Met Leu Gly 
        195                 200                 205             
Gln Gly Leu Glu Ala Val Met Gly Ile Ile Ala Arg Tyr Ala Asp Gln 
    210                 215                 220                 
Ile Asp Ala Gly Val Tyr Pro Ala Asp Leu Ala Thr Ile Asp Thr Gln 
225                 230                 235                 240 
Arg Ala Ala Met Gln His Leu Val Glu Glu Ser Arg Ala Ala Gly Ile 
                245                 250                 255     
Asn Gly Glu Leu Pro Ala Phe Val Thr Lys Leu Ala Asp Arg Ala Val 
            260                 265                 270         
Ala Glu Gly Arg Gly Gly Asp Ser Tyr Ala Ala Leu Ile Glu Gln Phe 
        275                 280                 285             
Arg Lys Ser Ala                                                 
    290                                                         

 
<210> 215
<211> 879
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Imine reductase variant from Pseudonocardia spinosispora
 
<400> 215
atgagtgatt cacctgtgcc cgtgacagtt ctgggtcttg gtctgatggg tcaggcctta 60
gcgggagcgt ttctggcagc cggtcaccct acgacggttt ggaatcgcag cgcagctaaa 120
gctgatgaac tggttggtcg cggtgcaacc ctggccgcgt ctcccgcggc tgccgtcgca 180
gcgggtagtc tgatcgttgt ttgcgtcaca gatcatcgtg cggtgcgcga actgctggaa 240
ccacttggcg atgcactgcc aggtcgcgtt ctggtcaacc tgacgagcgg tacgtcgcgt 300
gaagcccgtg agaacgcaga acatactgcg gctcagggag ccacctacct ggatggtgtg 360
attctgacca ttccaagcgg cattggcacc ccagaagcta cgattctgta ttcgggaccg 420
cgcagtacct tcgatgaaca tgaaagcgca ctgcgcgcat taggtccggg ggcgaccttt 480
gtaggggaag atcacggact ggctaacctg tatgatgcag caatgtcgag cgtgatgtgg 540
ggcctgctga atggtttcct gcatggaacc gcactgctgg gaaccgcggg cgtttcggca 600
acctctgtgg ttccgatgct gggtcaaggt tgggaagcga tgttgggttg gattgcgcgc 660
tacgcagatc agattgatgc cggtgtctat ccagcagacc tcgccacgat tgatacccag 720
cgtgctgcaa tgcagcacct ggtcgaggag agccgtgcgg cgggcatcaa tggcgaactt 780
ccggcgtttg tgaccaaact ggccgatcgc gcagtggcag aaggtcgtgg tggtgactct 840
tacgctgcac tgattgaaca gtttcgcaag tcagcttaa                        879

 
<210> 216
<211> 292
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Imine reductase variant from Pseudonocardia spinosispora
 
<400> 216
Met Ser Asp Ser Pro Val Pro Val Thr Val Leu Gly Leu Gly Leu Met 
1               5                   10                  15      
Gly Gln Ala Leu Ala Gly Ala Phe Leu Ala Ala Gly His Pro Thr Thr 
            20                  25                  30          
Val Trp Asn Arg Ser Ala Ala Lys Ala Asp Glu Leu Val Gly Arg Gly 
        35                  40                  45              
Ala Thr Leu Ala Ala Ser Pro Ala Ala Ala Val Ala Ala Gly Ser Leu 
    50                  55                  60                  
Ile Val Val Cys Val Thr Asp His Arg Ala Val Arg Glu Leu Leu Glu 
65                  70                  75                  80  
Pro Leu Gly Asp Ala Leu Pro Gly Arg Val Leu Val Asn Leu Thr Ser 
                85                  90                  95      
Gly Thr Ser Arg Glu Ala Arg Glu Asn Ala Glu His Thr Ala Ala Gln 
            100                 105                 110         
Gly Ala Thr Tyr Leu Asp Gly Val Ile Leu Thr Ile Pro Ser Gly Ile 
        115                 120                 125             
Gly Thr Pro Glu Ala Thr Ile Leu Tyr Ser Gly Pro Arg Ser Thr Phe 
    130                 135                 140                 
Asp Glu His Glu Ser Ala Leu Arg Ala Leu Gly Pro Gly Ala Thr Phe 
145                 150                 155                 160 
Val Gly Glu Asp His Gly Leu Ala Asn Leu Tyr Asp Ala Ala Met Ser 
                165                 170                 175     
Ser Val Met Trp Gly Leu Leu Asn Gly Phe Leu His Gly Thr Ala Leu 
            180                 185                 190         
Leu Gly Thr Ala Gly Val Ser Ala Thr Ser Val Val Pro Met Leu Gly 
        195                 200                 205             
Gln Gly Trp Glu Ala Met Leu Gly Trp Ile Ala Arg Tyr Ala Asp Gln 
    210                 215                 220                 
Ile Asp Ala Gly Val Tyr Pro Ala Asp Leu Ala Thr Ile Asp Thr Gln 
225                 230                 235                 240 
Arg Ala Ala Met Gln His Leu Val Glu Glu Ser Arg Ala Ala Gly Ile 
                245                 250                 255     
Asn Gly Glu Leu Pro Ala Phe Val Thr Lys Leu Ala Asp Arg Ala Val 
            260                 265                 270         
Ala Glu Gly Arg Gly Gly Asp Ser Tyr Ala Ala Leu Ile Glu Gln Phe 
        275                 280                 285             
Arg Lys Ser Ala                                                 
    290                                                         

 
<210> 217
<211> 879
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Imine reductase variant from Pseudonocardia spinosispora
 
<400> 217
atgagtgatt cacctgtgcc cgtgacagtt ctgggtcttg gtctgatggg tcaggcctta 60
gcgggagcgt ttctggcagc cggtcaccct acgacggttt ggaatcgcag cgcagctaaa 120
gctgatgaac tggttggtcg cggtgcaacc ctggccgcgt ctcccgcggc tgccgtcgca 180
gcgggtagtc tgatcgttgt ttgcgtcaca gatcatcgtg cggtgcgcga actgctggaa 240
ccacttggcg atgcactgcc aggtcgcgtt ctggtcaacc tgacgagcgg tacgtcgcgt 300
gaagcccgtg agaacgcaga acatactgcg gctcagggag ccacctacct ggatggtgtg 360
attctgacca ttccaagcgg cattggcacc ccagaagcta cgattctgta ttcgggaccg 420
cgcagtacct tcgatgaaca tgaaagcgca ctgcgcgcat taggtccggg ggcgaccttt 480
gtaggggaag atcacggact ggctgccctg tatgatgcag caatgttgag catgatgtgg 540
ggcctgctga atggtttcct gcatggaacc gcactgctgg gaaccgcggg cgtttcggca 600
acctctgtgg ttccgatgct gggtcaaggt ctagaagcga tgactggttg gattgcgcgc 660
tacgcagatc agattgatgc cggtgtctat ccagcagacg acgccacgat tgatacccag 720
cgtgctgcaa tgcagcacct ggtcgaggag agccgtgcgg cgggcatcaa tggcgaactt 780
ccggcgtttg tgaccaaact ggccgatcgc gcagtggcag aaggtcgtgg tggtgactct 840
tacgctgcac tgattgaaca gtttcgcaag tcagcttaa                        879

 
<210> 218
<211> 292
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Imine reductase variant from Pseudonocardia spinosispora
 
<400> 218
Met Ser Asp Ser Pro Val Pro Val Thr Val Leu Gly Leu Gly Leu Met 
1               5                   10                  15      
Gly Gln Ala Leu Ala Gly Ala Phe Leu Ala Ala Gly His Pro Thr Thr 
            20                  25                  30          
Val Trp Asn Arg Ser Ala Ala Lys Ala Asp Glu Leu Val Gly Arg Gly 
        35                  40                  45              
Ala Thr Leu Ala Ala Ser Pro Ala Ala Ala Val Ala Ala Gly Ser Leu 
    50                  55                  60                  
Ile Val Val Cys Val Thr Asp His Arg Ala Val Arg Glu Leu Leu Glu 
65                  70                  75                  80  
Pro Leu Gly Asp Ala Leu Pro Gly Arg Val Leu Val Asn Leu Thr Ser 
                85                  90                  95      
Gly Thr Ser Arg Glu Ala Arg Glu Asn Ala Glu His Thr Ala Ala Gln 
            100                 105                 110         
Gly Ala Thr Tyr Leu Asp Gly Val Ile Leu Thr Ile Pro Ser Gly Ile 
        115                 120                 125             
Gly Thr Pro Glu Ala Thr Ile Leu Tyr Ser Gly Pro Arg Ser Thr Phe 
    130                 135                 140                 
Asp Glu His Glu Ser Ala Leu Arg Ala Leu Gly Pro Gly Ala Thr Phe 
145                 150                 155                 160 
Val Gly Glu Asp His Gly Leu Ala Ala Leu Tyr Asp Ala Ala Met Leu 
                165                 170                 175     
Ser Met Met Trp Gly Leu Leu Asn Gly Phe Leu His Gly Thr Ala Leu 
            180                 185                 190         
Leu Gly Thr Ala Gly Val Ser Ala Thr Ser Val Val Pro Met Leu Gly 
        195                 200                 205             
Gln Gly Leu Glu Ala Met Thr Gly Trp Ile Ala Arg Tyr Ala Asp Gln 
    210                 215                 220                 
Ile Asp Ala Gly Val Tyr Pro Ala Asp Asp Ala Thr Ile Asp Thr Gln 
225                 230                 235                 240 
Arg Ala Ala Met Gln His Leu Val Glu Glu Ser Arg Ala Ala Gly Ile 
                245                 250                 255     
Asn Gly Glu Leu Pro Ala Phe Val Thr Lys Leu Ala Asp Arg Ala Val 
            260                 265                 270         
Ala Glu Gly Arg Gly Gly Asp Ser Tyr Ala Ala Leu Ile Glu Gln Phe 
        275                 280                 285             
Arg Lys Ser Ala                                                 
    290                                                         

 
<210> 219
<211> 879
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Imine reductase variant from Pseudonocardia spinosispora
 
<400> 219
atgagtgatt cacctgtgcc cgtgacagtt ctgggtcttg gtctgatggg tcaggcctta 60
gcgggagcgt ttctggcagc cggtcaccct acgacggttt ggaatcgcag cgcagctaaa 120
gctgatgaac tggttggtcg cggtgcaacc ctggccgcgt ctcccgcggc tgccgtcgca 180
gcgggtagtc tgatcgttgt ttgcgtcaca gatcatcgtg cggtgcgcga actgctggaa 240
ccacttggcg atgcactgcc aggtcgcgtt ctggtcaacc tgacgagcgg tacgtcgcgt 300
gaagcccgtg agaacgcaga acatactgcg gctcagggag ccacctacct ggatggtgtg 360
attctgacca ttccaagcgg cattggcacc ccagaagcta cgattctgta ttcgggaccg 420
cgcagtacct tcgatgaaca tgaaagcgca ctgcgcgcat taggtccggg ggcgaccttt 480
gtaggggaag atcacggact ggctgcactg tatgatgcag caatgtcgag cgttatgtgg 540
ggcctgctga atggtttcct gcatggaacc gcactgctgg gaaccgcggg cgtttcggca 600
acctctgtgg ttccgatgct gggtcaaggt ctagaagcga tgactggttg gattgcgcgc 660
tacgcagatc agattgatgc cggtgtctat ccagcagacg atgccacgat tgatacccag 720
cgtgctgcaa tgcagcacct ggtcgaggag agccgtgcgg cgggcatcaa tggcgaactt 780
ccggcgtttg tgaccaaact ggccgatcgc gcagtggcag aaggtcgtgg tggtgactct 840
tacgctgcac tgattgaaca gtttcgcaag tcagcttaa                        879

 
<210> 220
<211> 292
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Imine reductase variant from Pseudonocardia spinosispora
 
<400> 220
Met Ser Asp Ser Pro Val Pro Val Thr Val Leu Gly Leu Gly Leu Met 
1               5                   10                  15      
Gly Gln Ala Leu Ala Gly Ala Phe Leu Ala Ala Gly His Pro Thr Thr 
            20                  25                  30          
Val Trp Asn Arg Ser Ala Ala Lys Ala Asp Glu Leu Val Gly Arg Gly 
        35                  40                  45              
Ala Thr Leu Ala Ala Ser Pro Ala Ala Ala Val Ala Ala Gly Ser Leu 
    50                  55                  60                  
Ile Val Val Cys Val Thr Asp His Arg Ala Val Arg Glu Leu Leu Glu 
65                  70                  75                  80  
Pro Leu Gly Asp Ala Leu Pro Gly Arg Val Leu Val Asn Leu Thr Ser 
                85                  90                  95      
Gly Thr Ser Arg Glu Ala Arg Glu Asn Ala Glu His Thr Ala Ala Gln 
            100                 105                 110         
Gly Ala Thr Tyr Leu Asp Gly Val Ile Leu Thr Ile Pro Ser Gly Ile 
        115                 120                 125             
Gly Thr Pro Glu Ala Thr Ile Leu Tyr Ser Gly Pro Arg Ser Thr Phe 
    130                 135                 140                 
Asp Glu His Glu Ser Ala Leu Arg Ala Leu Gly Pro Gly Ala Thr Phe 
145                 150                 155                 160 
Val Gly Glu Asp His Gly Leu Ala Ala Leu Tyr Asp Ala Ala Met Ser 
                165                 170                 175     
Ser Val Met Trp Gly Leu Leu Asn Gly Phe Leu His Gly Thr Ala Leu 
            180                 185                 190         
Leu Gly Thr Ala Gly Val Ser Ala Thr Ser Val Val Pro Met Leu Gly 
        195                 200                 205             
Gln Gly Leu Glu Ala Met Thr Gly Trp Ile Ala Arg Tyr Ala Asp Gln 
    210                 215                 220                 
Ile Asp Ala Gly Val Tyr Pro Ala Asp Asp Ala Thr Ile Asp Thr Gln 
225                 230                 235                 240 
Arg Ala Ala Met Gln His Leu Val Glu Glu Ser Arg Ala Ala Gly Ile 
                245                 250                 255     
Asn Gly Glu Leu Pro Ala Phe Val Thr Lys Leu Ala Asp Arg Ala Val 
            260                 265                 270         
Ala Glu Gly Arg Gly Gly Asp Ser Tyr Ala Ala Leu Ile Glu Gln Phe 
        275                 280                 285             
Arg Lys Ser Ala                                                 
    290                                                         

 
<210> 221
<211> 879
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Imine reductase variant from Pseudonocardia spinosispora
 
<400> 221
atgagtgatt cacctgtgcc cgtgacagtt ctgggtcttg gtctgatggg tcaggcctta 60
gcgggagcgt ttctggcagc cggtcaccct acgacggttt ggaatcgcag cgcagctaaa 120
gctgatgaac tggttggtcg cggtgcaacc ctggccgcgt ctcccgcggc tgccgtcgca 180
gcgggtagtc tgatcgttgt ttgcgtcaca gatcatcgtg cggtgcgcga actgctggaa 240
ccacttggcg atgcactgcc aggtcgcgtt ctggtcaacc tgacgagcgg tacgtcgcgt 300
gaagcccgtg agaacgcaga acatactgcg gctcagggag ccacctacct ggatggtgtg 360
attctgacca ttccaagcgg cattggcacc ccagaagcta ggattctgta ttcgggaccg 420
cgcagtacct tcgatgaaca tgaaagcgca ctgcgcgcat taggtccggg ggcgaccttt 480
gtaggggaag atcacggact ggctaacctg tatgatgcag caatgtcgag catgatgtgg 540
ggcctgctga atggtttcct gcatggaacc gcactgctgg gaaccgcggg cgtttcggca 600
acctctgtgg ttccgatgct gggtcaaggt ctagaagcgg tgactggtct aattgcgcgc 660
tacgcagatc agattgatgc cggtgtctat ccagcagacg atgccacgat tgatacccag 720
cgtgctgcaa tgcagcacct ggtcgaggag agccgtgcgg cgggcatcaa tggcgaactt 780
ccggcgtttg tgaccaaact ggccgatcgc gcagtggcag aaggtcgtgg tggtgactct 840
tacgctgcac tgattgaaca gtttcgcaag tcagcttaa                        879

 
<210> 222
<211> 292
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Imine reductase variant from Pseudonocardia spinosispora
 
<400> 222
Met Ser Asp Ser Pro Val Pro Val Thr Val Leu Gly Leu Gly Leu Met 
1               5                   10                  15      
Gly Gln Ala Leu Ala Gly Ala Phe Leu Ala Ala Gly His Pro Thr Thr 
            20                  25                  30          
Val Trp Asn Arg Ser Ala Ala Lys Ala Asp Glu Leu Val Gly Arg Gly 
        35                  40                  45              
Ala Thr Leu Ala Ala Ser Pro Ala Ala Ala Val Ala Ala Gly Ser Leu 
    50                  55                  60                  
Ile Val Val Cys Val Thr Asp His Arg Ala Val Arg Glu Leu Leu Glu 
65                  70                  75                  80  
Pro Leu Gly Asp Ala Leu Pro Gly Arg Val Leu Val Asn Leu Thr Ser 
                85                  90                  95      
Gly Thr Ser Arg Glu Ala Arg Glu Asn Ala Glu His Thr Ala Ala Gln 
            100                 105                 110         
Gly Ala Thr Tyr Leu Asp Gly Val Ile Leu Thr Ile Pro Ser Gly Ile 
        115                 120                 125             
Gly Thr Pro Glu Ala Arg Ile Leu Tyr Ser Gly Pro Arg Ser Thr Phe 
    130                 135                 140                 
Asp Glu His Glu Ser Ala Leu Arg Ala Leu Gly Pro Gly Ala Thr Phe 
145                 150                 155                 160 
Val Gly Glu Asp His Gly Leu Ala Asn Leu Tyr Asp Ala Ala Met Ser 
                165                 170                 175     
Ser Met Met Trp Gly Leu Leu Asn Gly Phe Leu His Gly Thr Ala Leu 
            180                 185                 190         
Leu Gly Thr Ala Gly Val Ser Ala Thr Ser Val Val Pro Met Leu Gly 
        195                 200                 205             
Gln Gly Leu Glu Ala Val Thr Gly Leu Ile Ala Arg Tyr Ala Asp Gln 
    210                 215                 220                 
Ile Asp Ala Gly Val Tyr Pro Ala Asp Asp Ala Thr Ile Asp Thr Gln 
225                 230                 235                 240 
Arg Ala Ala Met Gln His Leu Val Glu Glu Ser Arg Ala Ala Gly Ile 
                245                 250                 255     
Asn Gly Glu Leu Pro Ala Phe Val Thr Lys Leu Ala Asp Arg Ala Val 
            260                 265                 270         
Ala Glu Gly Arg Gly Gly Asp Ser Tyr Ala Ala Leu Ile Glu Gln Phe 
        275                 280                 285             
Arg Lys Ser Ala                                                 
    290                                                         

 
<210> 223
<211> 879
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Imine reductase variant from Pseudonocardia spinosispora
 
<400> 223
atgagtgatt cacctgtgcc cgtgacagtt ctgggtcttg gtctgatggg tcaggcctta 60
gcgggagcgt ttctggcagc cggtcaccct acgacggttt ggaatcgcag cgcagctaaa 120
gctgatgaac tggttggtcg cggtgcaacc ctggccgcgt ctcccgcggc tgccgtcgca 180
gcgggtagtc tgatcgttgt ttgcgtcaca gatcatcgtg cggtgcgcga actgctggaa 240
ccacttggcg atgcactgcc aggtcgcgtt ctggtcaacc tgacgagcgg tacgtcgcgt 300
gaagcccgtg agaacgcaga acatactgcg gctcagggag ccacctacct ggatggtgtg 360
attctgacca ttccaagcgg cattggcacc ccagaagcta ggattctgta ttcgggaccg 420
cgcagtacct tcgatgaaca tgaaagcgca ctgcgcgcat taggtccggg ggcgaccttt 480
gtaggggaag atcacggact ggctaacctg tatgatgcag caatgttgag cgttatgtgg 540
ggcctgctga atggtttcct gcatggaacc gcactgctgg gaaccgcggg cgtttcggca 600
acctctgtgg ttccgatgct gggtcaaggt tgggaagcgg tgactggtct aattgcgcgc 660
tacgcagatc agattgatgc cggtgtctat ccagcagacg atgccacgat tgatacccag 720
cgtgctgcaa tgcagcacct ggtcgaggag agccgtgcgg cgggcatcaa tggcgaactt 780
ccggcgtttg tgaccaaact ggccgatcgc gcagtggcag aaggtcgtgg tggtgactct 840
tacgctgcac tgattgaaca gtttcgcaag tcagcttaa                        879

 
<210> 224
<211> 292
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Imine reductase variant from Pseudonocardia spinosispora
 
<400> 224
Met Ser Asp Ser Pro Val Pro Val Thr Val Leu Gly Leu Gly Leu Met 
1               5                   10                  15      
Gly Gln Ala Leu Ala Gly Ala Phe Leu Ala Ala Gly His Pro Thr Thr 
            20                  25                  30          
Val Trp Asn Arg Ser Ala Ala Lys Ala Asp Glu Leu Val Gly Arg Gly 
        35                  40                  45              
Ala Thr Leu Ala Ala Ser Pro Ala Ala Ala Val Ala Ala Gly Ser Leu 
    50                  55                  60                  
Ile Val Val Cys Val Thr Asp His Arg Ala Val Arg Glu Leu Leu Glu 
65                  70                  75                  80  
Pro Leu Gly Asp Ala Leu Pro Gly Arg Val Leu Val Asn Leu Thr Ser 
                85                  90                  95      
Gly Thr Ser Arg Glu Ala Arg Glu Asn Ala Glu His Thr Ala Ala Gln 
            100                 105                 110         
Gly Ala Thr Tyr Leu Asp Gly Val Ile Leu Thr Ile Pro Ser Gly Ile 
        115                 120                 125             
Gly Thr Pro Glu Ala Arg Ile Leu Tyr Ser Gly Pro Arg Ser Thr Phe 
    130                 135                 140                 
Asp Glu His Glu Ser Ala Leu Arg Ala Leu Gly Pro Gly Ala Thr Phe 
145                 150                 155                 160 
Val Gly Glu Asp His Gly Leu Ala Asn Leu Tyr Asp Ala Ala Met Leu 
                165                 170                 175     
Ser Val Met Trp Gly Leu Leu Asn Gly Phe Leu His Gly Thr Ala Leu 
            180                 185                 190         
Leu Gly Thr Ala Gly Val Ser Ala Thr Ser Val Val Pro Met Leu Gly 
        195                 200                 205             
Gln Gly Trp Glu Ala Val Thr Gly Leu Ile Ala Arg Tyr Ala Asp Gln 
    210                 215                 220                 
Ile Asp Ala Gly Val Tyr Pro Ala Asp Asp Ala Thr Ile Asp Thr Gln 
225                 230                 235                 240 
Arg Ala Ala Met Gln His Leu Val Glu Glu Ser Arg Ala Ala Gly Ile 
                245                 250                 255     
Asn Gly Glu Leu Pro Ala Phe Val Thr Lys Leu Ala Asp Arg Ala Val 
            260                 265                 270         
Ala Glu Gly Arg Gly Gly Asp Ser Tyr Ala Ala Leu Ile Glu Gln Phe 
        275                 280                 285             
Arg Lys Ser Ala                                                 
    290                                                         

 
<210> 225
<211> 879
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Imine reductase variant from Pseudonocardia spinosispora
 
<400> 225
atgagtgatt cacctgtgcc cgtgacagtt ctgggtcttg gtctgatggg tcaggcctta 60
gcgggagcgt ttctggcagc cggtcaccct acgacggttt ggaatcgcag cgcagctaaa 120
gctgatgaac tggttggtcg cggtgcaacc ctggccgcgt ctcccgcggc tgccgtcgca 180
gcgggtagtc tgatcgttgt ttgcgtcaca gatcatcgtg cggtgcgcga actgctggaa 240
ccacttggcg atgcactgcc aggtcgcgtt ctggtcaacc tgacgagcgg tacgtcgcgt 300
gaagcccgtg agaacgcaga acatactgcg gctcagggag ccacctacct ggatggtgtg 360
attctgacca ttccaagcgg cattggcacc ccagaagcta cgattctgta ttcgggaccg 420
cgcagtacct tcgatgaaca tgaaagcgca ctgcgcgcat taggtccggg ggcgaccttt 480
gtaggggaag atcacggact ggctaacctg tatgatgcag caatgtcgag cgttatgtgg 540
ggcctgctga atggtttcct gcatggaacc gcactgctgg gaaccgcggg cgtttcggca 600
acctctgtgg ttccgatgct gggtcaaggt tgggaagcgg tgactggtat aattgcgcgc 660
tacgcagatc agattgatgc cggtgtctat ccagcagacg atgccacgat tgatacccag 720
cgtgctgcaa tgcagcacct ggtcgaggag agccgtgcgg cgggcatcaa tggcgaactt 780
ccggcgtttg tgaccaaact ggccgatcgc gcagtggcag aaggtcgtgg tggtgactct 840
tacgctgcac tgattgaaca gtttcgcaag tcagcttaa                        879

 
<210> 226
<211> 292
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Imine reductase variant from Pseudonocardia spinosispora
 
<400> 226
Met Ser Asp Ser Pro Val Pro Val Thr Val Leu Gly Leu Gly Leu Met 
1               5                   10                  15      
Gly Gln Ala Leu Ala Gly Ala Phe Leu Ala Ala Gly His Pro Thr Thr 
            20                  25                  30          
Val Trp Asn Arg Ser Ala Ala Lys Ala Asp Glu Leu Val Gly Arg Gly 
        35                  40                  45              
Ala Thr Leu Ala Ala Ser Pro Ala Ala Ala Val Ala Ala Gly Ser Leu 
    50                  55                  60                  
Ile Val Val Cys Val Thr Asp His Arg Ala Val Arg Glu Leu Leu Glu 
65                  70                  75                  80  
Pro Leu Gly Asp Ala Leu Pro Gly Arg Val Leu Val Asn Leu Thr Ser 
                85                  90                  95      
Gly Thr Ser Arg Glu Ala Arg Glu Asn Ala Glu His Thr Ala Ala Gln 
            100                 105                 110         
Gly Ala Thr Tyr Leu Asp Gly Val Ile Leu Thr Ile Pro Ser Gly Ile 
        115                 120                 125             
Gly Thr Pro Glu Ala Thr Ile Leu Tyr Ser Gly Pro Arg Ser Thr Phe 
    130                 135                 140                 
Asp Glu His Glu Ser Ala Leu Arg Ala Leu Gly Pro Gly Ala Thr Phe 
145                 150                 155                 160 
Val Gly Glu Asp His Gly Leu Ala Asn Leu Tyr Asp Ala Ala Met Ser 
                165                 170                 175     
Ser Val Met Trp Gly Leu Leu Asn Gly Phe Leu His Gly Thr Ala Leu 
            180                 185                 190         
Leu Gly Thr Ala Gly Val Ser Ala Thr Ser Val Val Pro Met Leu Gly 
        195                 200                 205             
Gln Gly Trp Glu Ala Val Thr Gly Ile Ile Ala Arg Tyr Ala Asp Gln 
    210                 215                 220                 
Ile Asp Ala Gly Val Tyr Pro Ala Asp Asp Ala Thr Ile Asp Thr Gln 
225                 230                 235                 240 
Arg Ala Ala Met Gln His Leu Val Glu Glu Ser Arg Ala Ala Gly Ile 
                245                 250                 255     
Asn Gly Glu Leu Pro Ala Phe Val Thr Lys Leu Ala Asp Arg Ala Val 
            260                 265                 270         
Ala Glu Gly Arg Gly Gly Asp Ser Tyr Ala Ala Leu Ile Glu Gln Phe 
        275                 280                 285             
Arg Lys Ser Ala                                                 
    290                                                         

 
<210> 227
<211> 879
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Imine reductase variant from Pseudonocardia spinosispora
 
<400> 227
atgagtgatt cacctgtgcc cgtgacagtt ctgggtcttg gtctgatggg tcaggcctta 60
gcgggagcgt ttctggcagc cggtcaccct acgacggttt ggaatcgcag cgcagctaaa 120
gctgatgaac tggttggtcg cggtgcaacc ctggccgcgt ctcccgcggc tgccgtcgca 180
gcgggtagtc tgatcgttgt ttgcgtcaca gatcatcgtg cggtgcgcga actgctggaa 240
ccacttggcg atgcactgcc aggtcgcgtt ctggtcaacc tgacgagcgg tacgtcgcgt 300
gaagcccgtg agaacgcaga acatactgcg gctcagggag ccacctacct ggatggtgtg 360
attctgacca ttccaagcgg cattggcacc ccagaagcta cgattctgta ttcgggaccg 420
cgcagtacct tcgatgaaca tgaaagcgca ctgcgcgcat taggtccggg ggcgaccttt 480
gtaggggaag atcacggact ggctaacctg tatgatgcag caatgtcgag cattatgtgg 540
ggcctgctga atggtttcct gcatggaacc gcactgctgg gaaccgcggg cgtttcggca 600
acctctgtgg ttccgatgct gggtcaaggt ctagaagcga tgactggttg gattgcgcgc 660
tacgcagatc agattgatgc cggtgtctat ccagcagacg atgccacgat tgatacccag 720
cgtgctgcaa tgcagcacct ggtcgaggag agccgtgcgg cgggcatcaa tggcgaactt 780
ccggcgtttg tgaccaaact ggccgatcgc gcagtggcag aaggtcgtgg tggtgactct 840
tacgctgcac tgattgaaca gtttcgcaag tcagcttaa                        879

 
<210> 228
<211> 292
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Imine reductase variant from Pseudonocardia spinosispora
 
<400> 228
Met Ser Asp Ser Pro Val Pro Val Thr Val Leu Gly Leu Gly Leu Met 
1               5                   10                  15      
Gly Gln Ala Leu Ala Gly Ala Phe Leu Ala Ala Gly His Pro Thr Thr 
            20                  25                  30          
Val Trp Asn Arg Ser Ala Ala Lys Ala Asp Glu Leu Val Gly Arg Gly 
        35                  40                  45              
Ala Thr Leu Ala Ala Ser Pro Ala Ala Ala Val Ala Ala Gly Ser Leu 
    50                  55                  60                  
Ile Val Val Cys Val Thr Asp His Arg Ala Val Arg Glu Leu Leu Glu 
65                  70                  75                  80  
Pro Leu Gly Asp Ala Leu Pro Gly Arg Val Leu Val Asn Leu Thr Ser 
                85                  90                  95      
Gly Thr Ser Arg Glu Ala Arg Glu Asn Ala Glu His Thr Ala Ala Gln 
            100                 105                 110         
Gly Ala Thr Tyr Leu Asp Gly Val Ile Leu Thr Ile Pro Ser Gly Ile 
        115                 120                 125             
Gly Thr Pro Glu Ala Thr Ile Leu Tyr Ser Gly Pro Arg Ser Thr Phe 
    130                 135                 140                 
Asp Glu His Glu Ser Ala Leu Arg Ala Leu Gly Pro Gly Ala Thr Phe 
145                 150                 155                 160 
Val Gly Glu Asp His Gly Leu Ala Asn Leu Tyr Asp Ala Ala Met Ser 
                165                 170                 175     
Ser Ile Met Trp Gly Leu Leu Asn Gly Phe Leu His Gly Thr Ala Leu 
            180                 185                 190         
Leu Gly Thr Ala Gly Val Ser Ala Thr Ser Val Val Pro Met Leu Gly 
        195                 200                 205             
Gln Gly Leu Glu Ala Met Thr Gly Trp Ile Ala Arg Tyr Ala Asp Gln 
    210                 215                 220                 
Ile Asp Ala Gly Val Tyr Pro Ala Asp Asp Ala Thr Ile Asp Thr Gln 
225                 230                 235                 240 
Arg Ala Ala Met Gln His Leu Val Glu Glu Ser Arg Ala Ala Gly Ile 
                245                 250                 255     
Asn Gly Glu Leu Pro Ala Phe Val Thr Lys Leu Ala Asp Arg Ala Val 
            260                 265                 270         
Ala Glu Gly Arg Gly Gly Asp Ser Tyr Ala Ala Leu Ile Glu Gln Phe 
        275                 280                 285             
Arg Lys Ser Ala                                                 
    290                                                         

 
<210> 229
<211> 879
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Imine reductase variant from Pseudonocardia spinosispora
 
<400> 229
atgagtgatt cacctgtgcc cgtgacagtt ctgggtcttg gtctgatggg tcaggcctta 60
gcgggagcgt ttctggcagc cggtcaccct acgacggttt ggaatcgcag cgcagctaaa 120
gctgatgaac tggttggtcg cggtgcaacc ctggccgcgt ctcccgcggc tgccgtcgca 180
gcgggtagtc tgatcgttgt ttgcgtcaca gatcatcgtg cggtgcgcga actgctggaa 240
ccacttggcg atgcactgcc aggtcgcgtt ctggtcaacc tgacgagcgg tacgtcgcgt 300
gaagcccgtg agaacgcaga acatactgcg gctcagggag ccacctacct ggatggtgtg 360
attctgacca ttccaagcgg cattggcacc ccagaagcta ccattctgta ttcgggaccg 420
cgcagtacct tcgatgaaca tgaaagcgca ctgcgcgcat taggtccggg ggcgaccttt 480
gtaggggaag atcacggact ggctaacctg tatgatgcag caatgtcgag catgatgtgg 540
ggcctgctga atggtttcct gcatggaacc gcactgctgg gaaccgcggg cgtttcggca 600
acctctgtgg ttccgatgct gggtcaaggt tgggaagcga tgactggtat aattgcgcgc 660
tacgcagatc agattgatgc cggtgtctat ccagcagacg atgccacgat tgatacccag 720
cgtgctgcaa tgcagcacct ggtcgaggag agccgtgcgg cgggcatcaa tggcgaactt 780
ccggcgtttg tgaccaaact ggccgatcgc gcagtggcag aaggtcgtgg tggtgactct 840
tacgctgcac tgattgaaca gtttcgcaag tcagcttaa                        879

 
<210> 230
<211> 292
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Imine reductase variant from Pseudonocardia spinosispora
 
<400> 230
Met Ser Asp Ser Pro Val Pro Val Thr Val Leu Gly Leu Gly Leu Met 
1               5                   10                  15      
Gly Gln Ala Leu Ala Gly Ala Phe Leu Ala Ala Gly His Pro Thr Thr 
            20                  25                  30          
Val Trp Asn Arg Ser Ala Ala Lys Ala Asp Glu Leu Val Gly Arg Gly 
        35                  40                  45              
Ala Thr Leu Ala Ala Ser Pro Ala Ala Ala Val Ala Ala Gly Ser Leu 
    50                  55                  60                  
Ile Val Val Cys Val Thr Asp His Arg Ala Val Arg Glu Leu Leu Glu 
65                  70                  75                  80  
Pro Leu Gly Asp Ala Leu Pro Gly Arg Val Leu Val Asn Leu Thr Ser 
                85                  90                  95      
Gly Thr Ser Arg Glu Ala Arg Glu Asn Ala Glu His Thr Ala Ala Gln 
            100                 105                 110         
Gly Ala Thr Tyr Leu Asp Gly Val Ile Leu Thr Ile Pro Ser Gly Ile 
        115                 120                 125             
Gly Thr Pro Glu Ala Thr Ile Leu Tyr Ser Gly Pro Arg Ser Thr Phe 
    130                 135                 140                 
Asp Glu His Glu Ser Ala Leu Arg Ala Leu Gly Pro Gly Ala Thr Phe 
145                 150                 155                 160 
Val Gly Glu Asp His Gly Leu Ala Asn Leu Tyr Asp Ala Ala Met Ser 
                165                 170                 175     
Ser Met Met Trp Gly Leu Leu Asn Gly Phe Leu His Gly Thr Ala Leu 
            180                 185                 190         
Leu Gly Thr Ala Gly Val Ser Ala Thr Ser Val Val Pro Met Leu Gly 
        195                 200                 205             
Gln Gly Trp Glu Ala Met Thr Gly Ile Ile Ala Arg Tyr Ala Asp Gln 
    210                 215                 220                 
Ile Asp Ala Gly Val Tyr Pro Ala Asp Asp Ala Thr Ile Asp Thr Gln 
225                 230                 235                 240 
Arg Ala Ala Met Gln His Leu Val Glu Glu Ser Arg Ala Ala Gly Ile 
                245                 250                 255     
Asn Gly Glu Leu Pro Ala Phe Val Thr Lys Leu Ala Asp Arg Ala Val 
            260                 265                 270         
Ala Glu Gly Arg Gly Gly Asp Ser Tyr Ala Ala Leu Ile Glu Gln Phe 
        275                 280                 285             
Arg Lys Ser Ala                                                 
    290                                                         

 
<210> 231
<211> 879
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Imine reductase variant from Pseudonocardia spinosispora
 
<400> 231
atgagtgatt cacctgtgcc cgtgacagtt ctgggtcttg gtctgatggg tcaggcctta 60
gcgggagcgt ttctggcagc cggtcaccct acgacggttt ggaatcgcag cgcagctaaa 120
gctgatgaac tggttggtcg cggtgcaacc ctggccgcgt ctcccgcggc tgccgtcgca 180
gcgggtagtc tgatcgttgt ttgcgtcaca gatcatcgtg cggtgcgcga actgctggaa 240
ccacttggcg atgcactgcc aggtcgcgtt ctggtcaacc tgacgagcgg tacgtcgcgt 300
gaagcccgtg agaacgcaga acatactgcg gctcagggag ccacctacct ggatggtgtg 360
attctgacca ttccaagcgg cattggcacc ccagaagcta ggattctgta ttcgggaccg 420
cgcagtacct tcgatgaaca tgaaagcgca ctgcgcgcat taggtccggg ggcgaccttt 480
gtaggggaag atcacggact ggctaacctg tatgatgcag caatgtcgag cattatgtgg 540
ggcctgctga atggtttcct gcatggaacc gcactgctgg gaaccgcggg cgtttcggca 600
acctctgtgg ttccgatgct gggtcaaggt ctagaagcga tgactggttg gattgcgcgc 660
tacgcagatc agattgatgc cggtgtctat ccagcagacg atgccacgat tgatacccag 720
cgtgctgcaa tgcagcacct ggtcgaggag agccgtgcgg cgggcatcaa tggcgaactt 780
ccggcgtttg tgaccaaact ggccgatcgc gcagtggcag aaggtcgtgg tggtgactct 840
tacgctgcac tgattgaaca gtttcgcaag tcagcttaa                        879

 
<210> 232
<211> 292
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Imine reductase variant from Pseudonocardia spinosispora
 
<400> 232
Met Ser Asp Ser Pro Val Pro Val Thr Val Leu Gly Leu Gly Leu Met 
1               5                   10                  15      
Gly Gln Ala Leu Ala Gly Ala Phe Leu Ala Ala Gly His Pro Thr Thr 
            20                  25                  30          
Val Trp Asn Arg Ser Ala Ala Lys Ala Asp Glu Leu Val Gly Arg Gly 
        35                  40                  45              
Ala Thr Leu Ala Ala Ser Pro Ala Ala Ala Val Ala Ala Gly Ser Leu 
    50                  55                  60                  
Ile Val Val Cys Val Thr Asp His Arg Ala Val Arg Glu Leu Leu Glu 
65                  70                  75                  80  
Pro Leu Gly Asp Ala Leu Pro Gly Arg Val Leu Val Asn Leu Thr Ser 
                85                  90                  95      
Gly Thr Ser Arg Glu Ala Arg Glu Asn Ala Glu His Thr Ala Ala Gln 
            100                 105                 110         
Gly Ala Thr Tyr Leu Asp Gly Val Ile Leu Thr Ile Pro Ser Gly Ile 
        115                 120                 125             
Gly Thr Pro Glu Ala Arg Ile Leu Tyr Ser Gly Pro Arg Ser Thr Phe 
    130                 135                 140                 
Asp Glu His Glu Ser Ala Leu Arg Ala Leu Gly Pro Gly Ala Thr Phe 
145                 150                 155                 160 
Val Gly Glu Asp His Gly Leu Ala Asn Leu Tyr Asp Ala Ala Met Ser 
                165                 170                 175     
Ser Ile Met Trp Gly Leu Leu Asn Gly Phe Leu His Gly Thr Ala Leu 
            180                 185                 190         
Leu Gly Thr Ala Gly Val Ser Ala Thr Ser Val Val Pro Met Leu Gly 
        195                 200                 205             
Gln Gly Leu Glu Ala Met Thr Gly Trp Ile Ala Arg Tyr Ala Asp Gln 
    210                 215                 220                 
Ile Asp Ala Gly Val Tyr Pro Ala Asp Asp Ala Thr Ile Asp Thr Gln 
225                 230                 235                 240 
Arg Ala Ala Met Gln His Leu Val Glu Glu Ser Arg Ala Ala Gly Ile 
                245                 250                 255     
Asn Gly Glu Leu Pro Ala Phe Val Thr Lys Leu Ala Asp Arg Ala Val 
            260                 265                 270         
Ala Glu Gly Arg Gly Gly Asp Ser Tyr Ala Ala Leu Ile Glu Gln Phe 
        275                 280                 285             
Arg Lys Ser Ala                                                 
    290                                                         

	
<210> 233
<211> 879
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Imine reductase variant from Pseudonocardia spinosispora
 
<400> 233
atgagtgatt cacctgtgcc cgtgacagtt ctgggtcttg gtctgatggg tcaggcctta 60
gcgggagcgt ttctggcagc cggtcaccct acgacggttt ggaatcgcag cgcagctaaa 120
gctgatgaac tggttggtcg cggtgcaacc ctggccgcgt ctcccgcggc tgccgtcgca 180
gcgggtagtc tgatcgttgt ttgcgtcaca gatcatcgtg cggtgcgcga actgctggaa 240
ccacttggcg atgcactgcc aggtcgcgtt ctggtcaacc tgacgagcgg tacgtcgcgt 300
gaagcccgtg agaacgcaga acatactgcg gctcagggag ccacctacct ggatggtgtg 360
attctgacca ttccaagcgg cattggcacc ccagaagcta ccattctgta ttcgggaccg 420
cgcagtacct tcgatgaaca tgaaagcgca ctgcgcgcat taggtccggg ggcgaccttt 480
gtaggggaag atcacggact ggctaacctg tatgatgcag caatgtcgag catgatgtgg 540
ggcctgctga atggtttcct gcatggaacc gcactgctgg gaaccgcggg cgtttcggca 600
acctctgtgg ttccgatgct gggtcaaggt tgggaagcga tgactggtat aattgcgcgc 660
tacgcaaatc agattgatgc cggtgtctat ccagcagacg atgccacgat tgatacccag 720
cgtgctgcaa tgcagcacct ggtcgaggag agccgtgcgg cgggcatcaa tggcgaactt 780
ccggcgtttg tgaccaaact ggccgatcgc gcagtggcag aaggtcgtgg tggtgactct 840
tacgctgcac tgattgaaca gtttcgcaag tcagcttaa                        879

 
<210> 234
<211> 292
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Imine reductase variant from Pseudonocardia spinosispora
 
<400> 234
Met Ser Asp Ser Pro Val Pro Val Thr Val Leu Gly Leu Gly Leu Met 
1               5                   10                  15      
Gly Gln Ala Leu Ala Gly Ala Phe Leu Ala Ala Gly His Pro Thr Thr 
            20                  25                  30          
Val Trp Asn Arg Ser Ala Ala Lys Ala Asp Glu Leu Val Gly Arg Gly 
        35                  40                  45              
Ala Thr Leu Ala Ala Ser Pro Ala Ala Ala Val Ala Ala Gly Ser Leu 
    50                  55                  60                  
Ile Val Val Cys Val Thr Asp His Arg Ala Val Arg Glu Leu Leu Glu 
65                  70                  75                  80  
Pro Leu Gly Asp Ala Leu Pro Gly Arg Val Leu Val Asn Leu Thr Ser 
                85                  90                  95      
Gly Thr Ser Arg Glu Ala Arg Glu Asn Ala Glu His Thr Ala Ala Gln 
            100                 105                 110         
Gly Ala Thr Tyr Leu Asp Gly Val Ile Leu Thr Ile Pro Ser Gly Ile 
        115                 120                 125             
Gly Thr Pro Glu Ala Thr Ile Leu Tyr Ser Gly Pro Arg Ser Thr Phe 
    130                 135                 140                 
Asp Glu His Glu Ser Ala Leu Arg Ala Leu Gly Pro Gly Ala Thr Phe 
145                 150                 155                 160 
Val Gly Glu Asp His Gly Leu Ala Asn Leu Tyr Asp Ala Ala Met Ser 
                165                 170                 175     
Ser Met Met Trp Gly Leu Leu Asn Gly Phe Leu His Gly Thr Ala Leu 
            180                 185                 190         
Leu Gly Thr Ala Gly Val Ser Ala Thr Ser Val Val Pro Met Leu Gly 
        195                 200                 205             
Gln Gly Trp Glu Ala Met Thr Gly Ile Ile Ala Arg Tyr Ala Asn Gln 
    210                 215                 220                 
Ile Asp Ala Gly Val Tyr Pro Ala Asp Asp Ala Thr Ile Asp Thr Gln 
225                 230                 235                 240 
Arg Ala Ala Met Gln His Leu Val Glu Glu Ser Arg Ala Ala Gly Ile 
                245                 250                 255     
Asn Gly Glu Leu Pro Ala Phe Val Thr Lys Leu Ala Asp Arg Ala Val 
            260                 265                 270         
Ala Glu Gly Arg Gly Gly Asp Ser Tyr Ala Ala Leu Ile Glu Gln Phe 
        275                 280                 285             
Arg Lys Ser Ala                                                 
    290                                                         

 
<210> 235
<211> 879
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Imine reductase variant from Pseudonocardia spinosispora
 
<400> 235
atgagtgatt cacctgtgcc cgtgacagtt ctgggtcttg gtctgatggg tcaggcctta 60
gcgggagcgt ttctggcagc cggtcaccct acgacggttt ggaatcgcag cgcagctaaa 120
gctgatgaac tggttggtcg cggtgcaacc ctggccgcgt ctcccgcggc tgccgtcgca 180
gcgggtagtc tgatcgttgt ttgcgtcaca gatcatcgtg cggtgcgcga actgctggaa 240
ccacttggcg atgcactgcc aggtcgcgtt ctggtcaacc tgacgagcgg tacgtcgcgt 300
gaagcccgtg agaacgcaga acatactgcg gctcagggag ccacctacct ggatggtgtg 360
attctgacca ttccaagcgg cattggcacc ccagaagcta ccattctgta ttcgggaccg 420
cgcagtacct tcgatgaaca tgaaagcgca ctgcgcgcat taggtccggg ggcgaccttt 480
gtaggggaag atcacggact ggctaacctg tatgatgcag caatgtcgag catgatgtgg 540
ggcctgctga atggtttcct gcatggaacc gcactgctgg gaaccgcggg cgtttcggca 600
acctctgtgg ttccgatgct gggtcaaggt tgggaagcga tgactggtat aattgcgcgc 660
tacgcagatc agattgatgc cggtgtctat ccagcagacg atgccacgat tgatacccag 720
cgtgctgcaa tgcagcacct ggtcgaggag agccgtgcgg cgggcatcaa tggcgaactt 780
ccggcgtttg tgaccaaact ggccgatcgc gcagtggcag aaggtcgtgg tggtgtttct 840
tacgctgcac tgattgaaca gtttcgcaag tcagcttaa                        879

 
<210> 236
<211> 292
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Imine reductase variant from Pseudonocardia spinosispora
 
<400> 236
Met Ser Asp Ser Pro Val Pro Val Thr Val Leu Gly Leu Gly Leu Met 
1               5                   10                  15      
Gly Gln Ala Leu Ala Gly Ala Phe Leu Ala Ala Gly His Pro Thr Thr 
            20                  25                  30          
Val Trp Asn Arg Ser Ala Ala Lys Ala Asp Glu Leu Val Gly Arg Gly 
        35                  40                  45              
Ala Thr Leu Ala Ala Ser Pro Ala Ala Ala Val Ala Ala Gly Ser Leu 
    50                  55                  60                  
Ile Val Val Cys Val Thr Asp His Arg Ala Val Arg Glu Leu Leu Glu 
65                  70                  75                  80  
Pro Leu Gly Asp Ala Leu Pro Gly Arg Val Leu Val Asn Leu Thr Ser 
                85                  90                  95      
Gly Thr Ser Arg Glu Ala Arg Glu Asn Ala Glu His Thr Ala Ala Gln 
            100                 105                 110         
Gly Ala Thr Tyr Leu Asp Gly Val Ile Leu Thr Ile Pro Ser Gly Ile 
        115                 120                 125             
Gly Thr Pro Glu Ala Thr Ile Leu Tyr Ser Gly Pro Arg Ser Thr Phe 
    130                 135                 140                 
Asp Glu His Glu Ser Ala Leu Arg Ala Leu Gly Pro Gly Ala Thr Phe 
145                 150                 155                 160 
Val Gly Glu Asp His Gly Leu Ala Asn Leu Tyr Asp Ala Ala Met Ser 
                165                 170                 175     
Ser Met Met Trp Gly Leu Leu Asn Gly Phe Leu His Gly Thr Ala Leu 
            180                 185                 190         
Leu Gly Thr Ala Gly Val Ser Ala Thr Ser Val Val Pro Met Leu Gly 
        195                 200                 205             
Gln Gly Trp Glu Ala Met Thr Gly Ile Ile Ala Arg Tyr Ala Asp Gln 
    210                 215                 220                 
Ile Asp Ala Gly Val Tyr Pro Ala Asp Asp Ala Thr Ile Asp Thr Gln 
225                 230                 235                 240 
Arg Ala Ala Met Gln His Leu Val Glu Glu Ser Arg Ala Ala Gly Ile 
                245                 250                 255     
Asn Gly Glu Leu Pro Ala Phe Val Thr Lys Leu Ala Asp Arg Ala Val 
            260                 265                 270         
Ala Glu Gly Arg Gly Gly Val Ser Tyr Ala Ala Leu Ile Glu Gln Phe 
        275                 280                 285             
Arg Lys Ser Ala                                                 
    290                                                         

 
<210> 237
<211> 879
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Imine reductase variant from Pseudonocardia spinosispora
 
<400> 237
atgagtgatt cacctgtgcc cgtgacagtt ctgggtcttg gtctgatggg tcaggcctta 60
gcgggagcgt ttctggcagc cggtcaccct acgacggttt ggaatcgcag cgcagctaaa 120
gctgatgaac tggttggtcg cggtgcaacc ctggccgcgt ctcccgcggc tgccgtcgca 180
gcgggtagtc tgatcgttgt ttgcgtcaca gatcatcgtg cggtgcgcga actgctggaa 240
ccacttggcg atgcactgcc aggtcgcgtt ctggtcaacc tgacgagcgg tacgtcgcgt 300
gaagcccgtg agaacgcaga acatactgcg gctcagggag ccacctacct ggatggtgtg 360
attctgacca ttccaagcgg cattggcacc ccagaagcta ccattctgta ttcgggaccg 420
cgcagtacct tcgatgaaca tgaaagcgca ctgcgcgcat taggtccggg ggcgaccttt 480
gtaggggaag atcacggact ggctaacctg tatgatgcag caatgtcgag catgatgtgg 540
ggcctgctga atggtttcct gcatggaacc gcactgctgg gaaccgcggg cgtttcggca 600
acctctgtgg ttccgatgct gggtcaaggt tgggaagcga tgactggtat aattgcgcgc 660
tacgcagatc agattgatgt tggtgtctat ccagcagacg atgccacgat tgatacccag 720
cgtgctgcaa tgcagcacct ggtcgaggag agccgtgcgg cgggcatcaa tggcgaactt 780
ccggcgtttg tgaccaaact ggccgatcgc gcagtggcag aaggtcgtgg tggtgactct 840
tacgctgcac tgattgaaca gtttcgcaag tcagcttaa                        879

 
<210> 238
<211> 292
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Imine reductase variant from Pseudonocardia spinosispora
 
<400> 238
Met Ser Asp Ser Pro Val Pro Val Thr Val Leu Gly Leu Gly Leu Met 
1               5                   10                  15      
Gly Gln Ala Leu Ala Gly Ala Phe Leu Ala Ala Gly His Pro Thr Thr 
            20                  25                  30          
Val Trp Asn Arg Ser Ala Ala Lys Ala Asp Glu Leu Val Gly Arg Gly 
        35                  40                  45              
Ala Thr Leu Ala Ala Ser Pro Ala Ala Ala Val Ala Ala Gly Ser Leu 
    50                  55                  60                  
Ile Val Val Cys Val Thr Asp His Arg Ala Val Arg Glu Leu Leu Glu 
65                  70                  75                  80  
Pro Leu Gly Asp Ala Leu Pro Gly Arg Val Leu Val Asn Leu Thr Ser 
                85                  90                  95      
Gly Thr Ser Arg Glu Ala Arg Glu Asn Ala Glu His Thr Ala Ala Gln 
            100                 105                 110         
Gly Ala Thr Tyr Leu Asp Gly Val Ile Leu Thr Ile Pro Ser Gly Ile 
        115                 120                 125             
Gly Thr Pro Glu Ala Thr Ile Leu Tyr Ser Gly Pro Arg Ser Thr Phe 
    130                 135                 140                 
Asp Glu His Glu Ser Ala Leu Arg Ala Leu Gly Pro Gly Ala Thr Phe 
145                 150                 155                 160 
Val Gly Glu Asp His Gly Leu Ala Asn Leu Tyr Asp Ala Ala Met Ser 
                165                 170                 175     
Ser Met Met Trp Gly Leu Leu Asn Gly Phe Leu His Gly Thr Ala Leu 
            180                 185                 190         
Leu Gly Thr Ala Gly Val Ser Ala Thr Ser Val Val Pro Met Leu Gly 
        195                 200                 205             
Gln Gly Trp Glu Ala Met Thr Gly Ile Ile Ala Arg Tyr Ala Asp Gln 
    210                 215                 220                 
Ile Asp Val Gly Val Tyr Pro Ala Asp Asp Ala Thr Ile Asp Thr Gln 
225                 230                 235                 240 
Arg Ala Ala Met Gln His Leu Val Glu Glu Ser Arg Ala Ala Gly Ile 
                245                 250                 255     
Asn Gly Glu Leu Pro Ala Phe Val Thr Lys Leu Ala Asp Arg Ala Val 
            260                 265                 270         
Ala Glu Gly Arg Gly Gly Asp Ser Tyr Ala Ala Leu Ile Glu Gln Phe 
        275                 280                 285             
Arg Lys Ser Ala                                                 
    290                                                         

 
<210> 239
<211> 879
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Imine reductase variant from Pseudonocardia spinosispora
 
<400> 239
atgagtgatt cacctgtgcc cgtgacagtt ctgggtcttg gtctgatggg tcaggcctta 60
gcgggagcgt ttctggcagc cggtcaccct acgacggttt ggaatcgcag cgcagctaaa 120
gctgatgaac tggttggtcg cggtgcaacc ctggccgcgt ctcccgcggc tgccgtcgca 180
gcgggtagtc tgatcgttgt ttgcgtcaca gatcatcgtg cggtgcgcga actgctggaa 240
ccacttggcg atgcactgcc aggtcgcgtt ctggtcaacc tgacgagcgg tacgtcgcgt 300
gaagcccgtg agaacgcaga acatactgcg gctcagggag ccacctacct ggatggtgtg 360
attctgacca ttccaagcag gattggcacc ccagaagcta ccattctgta ttcgggaccg 420
cgcagtacct tcgatgaaca tgaaagcgca ctgcgcgcat taggtccggg ggcgaccttt 480
gtaggggaag atcacggact ggctaacctg tatgatgcag caatgtcgag catgatgtgg 540
ggcctgctga atggtttcct gcatggaacc gcactgctgg gaaccgcggg cgtttcggca 600
acctctgtgg ttccgatgct gggtcaaggt tgggaagcga tgactggtat aattgcgcgc 660
tacgcagatc agattgatgc cggtgtctat ccagcagacg atgccacgat tgatacccag 720
cgtgctgcaa tgcagcacct ggtcgaggag agccgtgcgg cgggcatcaa tggcgaactt 780
ccggcgtttg tgaccaaact ggccgatcgc gcagtggcag aaggtcgtgg tggtgactct 840
tacgctgcac tgattgaaca gtttcgcaag tcagcttaa                        879

 
<210> 240
<211> 292
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Imine reductase variant from Pseudonocardia spinosispora
 
<400> 240
Met Ser Asp Ser Pro Val Pro Val Thr Val Leu Gly Leu Gly Leu Met 
1               5                   10                  15      
Gly Gln Ala Leu Ala Gly Ala Phe Leu Ala Ala Gly His Pro Thr Thr 
            20                  25                  30          
Val Trp Asn Arg Ser Ala Ala Lys Ala Asp Glu Leu Val Gly Arg Gly 
        35                  40                  45              
Ala Thr Leu Ala Ala Ser Pro Ala Ala Ala Val Ala Ala Gly Ser Leu 
    50                  55                  60                  
Ile Val Val Cys Val Thr Asp His Arg Ala Val Arg Glu Leu Leu Glu 
65                  70                  75                  80  
Pro Leu Gly Asp Ala Leu Pro Gly Arg Val Leu Val Asn Leu Thr Ser 
                85                  90                  95      
Gly Thr Ser Arg Glu Ala Arg Glu Asn Ala Glu His Thr Ala Ala Gln 
            100                 105                 110         
Gly Ala Thr Tyr Leu Asp Gly Val Ile Leu Thr Ile Pro Ser Arg Ile 
        115                 120                 125             
Gly Thr Pro Glu Ala Thr Ile Leu Tyr Ser Gly Pro Arg Ser Thr Phe 
    130                 135                 140                 
Asp Glu His Glu Ser Ala Leu Arg Ala Leu Gly Pro Gly Ala Thr Phe 
145                 150                 155                 160 
Val Gly Glu Asp His Gly Leu Ala Asn Leu Tyr Asp Ala Ala Met Ser 
                165                 170                 175     
Ser Met Met Trp Gly Leu Leu Asn Gly Phe Leu His Gly Thr Ala Leu 
            180                 185                 190         
Leu Gly Thr Ala Gly Val Ser Ala Thr Ser Val Val Pro Met Leu Gly 
        195                 200                 205             
Gln Gly Trp Glu Ala Met Thr Gly Ile Ile Ala Arg Tyr Ala Asp Gln 
    210                 215                 220                 
Ile Asp Ala Gly Val Tyr Pro Ala Asp Asp Ala Thr Ile Asp Thr Gln 
225                 230                 235                 240 
Arg Ala Ala Met Gln His Leu Val Glu Glu Ser Arg Ala Ala Gly Ile 
                245                 250                 255     
Asn Gly Glu Leu Pro Ala Phe Val Thr Lys Leu Ala Asp Arg Ala Val 
            260                 265                 270         
Ala Glu Gly Arg Gly Gly Asp Ser Tyr Ala Ala Leu Ile Glu Gln Phe 
        275                 280                 285             
Arg Lys Ser Ala                                                 
    290                                                         

 
<210> 241
<211> 879
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Imine reductase variant from Pseudonocardia spinosispora
 
<400> 241
atgagtgatt cacctgtgcc cgtgacagtt ctgggtcttg gtctgatggg tcaggcctta 60
gcgggagcgt ttctggcagc cggtcaccct acgacggttt ggaatcgcag cgcagctaaa 120
gctgatgaac tggttggtcg cggtgcaacc ctggccgcgt ctcccgcggc tgccgtcgca 180
gcgggtagtc tgatcgttgt ttgcgtcaca gatcatcgtg cggtgcgcga actgctggaa 240
ccacttggcg atgcactgcc aggtcgcgtt ctggtcaacc tgacgagcgg tacgtcgcgt 300
gaagcccgtg agaacgcaga acatactgcg gctcagggag ccacctacct ggatggtgtg 360
attctgacca ttccaagcgg cattggcacc ccagaagcta ccattctgta ttcgggaccg 420
cgcagtacct tcgatgaaca tgaaagcgca ctgcgcgcat taggtccggg ggcgaccttt 480
gtaggggaag atcacggact ggctaacctg tatgatgcag caatgtcgag catgatgtgg 540
ggcctgctga atggtttcct gcatggaacc gcactgctgg gaaccgcggg cgtttcggca 600
acctctgtgg ttccgatgct gggtcaaggt tgggaagcga tgactagtat aattgcgcgc 660
tacgcagatc agattgatgc cggtgtctat ccagcagacg atgccacgat tgatacccag 720
cgtgctgcaa tgcagcacct ggtcgaggag agccgtgcgg cgggcatcaa tggcgaactt 780
ccggcgtttg tgaccaaact ggccgatcgc gcagtggcag aaggtcgtgg tggtgactct 840
tacgctgcac tgattgaaca gtttcgcaag tcagcttaa                        879

 
<210> 242
<211> 292
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Imine reductase variant from Pseudonocardia spinosispora
 
<400> 242
Met Ser Asp Ser Pro Val Pro Val Thr Val Leu Gly Leu Gly Leu Met 
1               5                   10                  15      
Gly Gln Ala Leu Ala Gly Ala Phe Leu Ala Ala Gly His Pro Thr Thr 
            20                  25                  30          
Val Trp Asn Arg Ser Ala Ala Lys Ala Asp Glu Leu Val Gly Arg Gly 
        35                  40                  45              
Ala Thr Leu Ala Ala Ser Pro Ala Ala Ala Val Ala Ala Gly Ser Leu 
    50                  55                  60                  
Ile Val Val Cys Val Thr Asp His Arg Ala Val Arg Glu Leu Leu Glu 
65                  70                  75                  80  
Pro Leu Gly Asp Ala Leu Pro Gly Arg Val Leu Val Asn Leu Thr Ser 
                85                  90                  95      
Gly Thr Ser Arg Glu Ala Arg Glu Asn Ala Glu His Thr Ala Ala Gln 
            100                 105                 110         
Gly Ala Thr Tyr Leu Asp Gly Val Ile Leu Thr Ile Pro Ser Gly Ile 
        115                 120                 125             
Gly Thr Pro Glu Ala Thr Ile Leu Tyr Ser Gly Pro Arg Ser Thr Phe 
    130                 135                 140                 
Asp Glu His Glu Ser Ala Leu Arg Ala Leu Gly Pro Gly Ala Thr Phe 
145                 150                 155                 160 
Val Gly Glu Asp His Gly Leu Ala Asn Leu Tyr Asp Ala Ala Met Ser 
                165                 170                 175     
Ser Met Met Trp Gly Leu Leu Asn Gly Phe Leu His Gly Thr Ala Leu 
            180                 185                 190         
Leu Gly Thr Ala Gly Val Ser Ala Thr Ser Val Val Pro Met Leu Gly 
        195                 200                 205             
Gln Gly Trp Glu Ala Met Thr Ser Ile Ile Ala Arg Tyr Ala Asp Gln 
    210                 215                 220                 
Ile Asp Ala Gly Val Tyr Pro Ala Asp Asp Ala Thr Ile Asp Thr Gln 
225                 230                 235                 240 
Arg Ala Ala Met Gln His Leu Val Glu Glu Ser Arg Ala Ala Gly Ile 
                245                 250                 255     
Asn Gly Glu Leu Pro Ala Phe Val Thr Lys Leu Ala Asp Arg Ala Val 
            260                 265                 270         
Ala Glu Gly Arg Gly Gly Asp Ser Tyr Ala Ala Leu Ile Glu Gln Phe 
        275                 280                 285             
Arg Lys Ser Ala                                                 
    290                                                         

 
<210> 243
<211> 879
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Imine reductase variant from Pseudonocardia spinosispora
 
<400> 243
atgagtgatt cacctgtgcc cgtgacagtt ctgggtcttg gtctgatggg tcaggcctta 60
gcgggagcgt ttctggcagc cggtcaccct acgacggttt ggaatcgcag cgcagctaaa 120
gctgatgaac tggttggtcg cggtgcaacc ctggccgcgt ctcccgcggc tgccgtcgca 180
gcgggtagtc tgatcgttgt ttgcgtcaca gatcatcgtg cggtgcgcga actgctggaa 240
ccacttggcg atgcactgcc aggtcgcgtt ctggtcaacc tgacgagcgg tacgtcgcgt 300
gaagcccgtg agaacgcaga acatactgcg gctcagggag ccacctacct ggatggtgtg 360
attctgacca ttccaagcgg cattggcacc ccagaagcta ccattctgta ttcgggaccg 420
cgcagtacct tcgatgaaca tgaaagcgca ctgcgcgcat taggtccggg ggcgaccttt 480
gtaggggaag atcacggact ggctaacctg tatgatgcaa tgatgtcgag catgatgtgg 540
ggcctgctga atggtttcct gcatggaacc gcactgctgg gaaccgcggg cgtttcggca 600
acctctgtgg ttccgatgct gggtcaaggt tgggaagcga tgactggtat aattgcgcgc 660
tacgcagatc agattgatgc cggtgtctat ccagcagacg atgccacgat tgatacccag 720
cgtgctgcaa tgcagcacct ggtcgaggag agccgtgcgg cgggcatcaa tggcgaactt 780
ccggcgtttg tgaccaaact ggccgatcgc gcagtggcag aaggtcgtgg tggtgactct 840
tacgctgcac tgattgaaca gtttcgcaag tcagcttaa                        879

 
<210> 244
<211> 292
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Imine reductase variant from Pseudonocardia spinosispora
 
<400> 244
Met Ser Asp Ser Pro Val Pro Val Thr Val Leu Gly Leu Gly Leu Met 
1               5                   10                  15      
Gly Gln Ala Leu Ala Gly Ala Phe Leu Ala Ala Gly His Pro Thr Thr 
            20                  25                  30          
Val Trp Asn Arg Ser Ala Ala Lys Ala Asp Glu Leu Val Gly Arg Gly 
        35                  40                  45              
Ala Thr Leu Ala Ala Ser Pro Ala Ala Ala Val Ala Ala Gly Ser Leu 
    50                  55                  60                  
Ile Val Val Cys Val Thr Asp His Arg Ala Val Arg Glu Leu Leu Glu 
65                  70                  75                  80  
Pro Leu Gly Asp Ala Leu Pro Gly Arg Val Leu Val Asn Leu Thr Ser 
                85                  90                  95      
Gly Thr Ser Arg Glu Ala Arg Glu Asn Ala Glu His Thr Ala Ala Gln 
            100                 105                 110         
Gly Ala Thr Tyr Leu Asp Gly Val Ile Leu Thr Ile Pro Ser Gly Ile 
        115                 120                 125             
Gly Thr Pro Glu Ala Thr Ile Leu Tyr Ser Gly Pro Arg Ser Thr Phe 
    130                 135                 140                 
Asp Glu His Glu Ser Ala Leu Arg Ala Leu Gly Pro Gly Ala Thr Phe 
145                 150                 155                 160 
Val Gly Glu Asp His Gly Leu Ala Asn Leu Tyr Asp Ala Met Met Ser 
                165                 170                 175     
Ser Met Met Trp Gly Leu Leu Asn Gly Phe Leu His Gly Thr Ala Leu 
            180                 185                 190         
Leu Gly Thr Ala Gly Val Ser Ala Thr Ser Val Val Pro Met Leu Gly 
        195                 200                 205             
Gln Gly Trp Glu Ala Met Thr Gly Ile Ile Ala Arg Tyr Ala Asp Gln 
    210                 215                 220                 
Ile Asp Ala Gly Val Tyr Pro Ala Asp Asp Ala Thr Ile Asp Thr Gln 
225                 230                 235                 240 
Arg Ala Ala Met Gln His Leu Val Glu Glu Ser Arg Ala Ala Gly Ile 
                245                 250                 255     
Asn Gly Glu Leu Pro Ala Phe Val Thr Lys Leu Ala Asp Arg Ala Val 
            260                 265                 270         
Ala Glu Gly Arg Gly Gly Asp Ser Tyr Ala Ala Leu Ile Glu Gln Phe 
        275                 280                 285             
Arg Lys Ser Ala                                                 
    290                                                         

 
<210> 245
<211> 879
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Imine reductase variant from Pseudonocardia spinosispora
 
<400> 245
atgagtgatt cacctgtgcc cgtgacagtt ctgggtcttg gtctgatggg tcaggcctta 60
gcgggagcgt ttctggcagc cggtcaccct acgacggttt ggaatcgcag cgcagctaaa 120
gctgatgaac tggttggtcg cggtgcaacc ctggccgcgt ctcccgcggc tgccgtcgca 180
gcgggtagtc tgatcgttgt ttgcgtcaca gatcatcgtg cggtgcgcga actgctggaa 240
ccacttggcg atgcactgcc aggtcgcgtt ctggtcaacc tgacgagcgg tacgtcgcgt 300
gaagcccgtg agaacgcaga acatactgcg gctcagggag ccacctacct ggatggtgtg 360
attctgacca ttccaagcgg cattggcacc ccagaagcta ccattctgta ttcgggaccg 420
cgcagtacct tcgatgaaca tgaaagcgca ctgcgcgcat taggtccggg ggcgaccttt 480
gtaggggaag atcacggact ggctaacctg tatgatgcag caatgtcgag catgatgtgg 540
ggcctgctga atggtttcct gcatggaacc gcactgctgg gaaccgcggg cgtttcggca 600
acctctgtgg ttccgatgct gggtcaaggt tggcatgcga tgactggtat aattgcgcgc 660
tacgcagatc agattgatgc cggtgtctat ccagcagacg atgccacgat tgatacccag 720
cgtgctgcaa tgcagcacct ggtcgaggag agccgtgcgg cgggcatcaa tggcgaactt 780
ccggcgtttg tgaccaaact ggccgatcgc gcagtggcag aaggtcgtgg tggtgactct 840
tacgctgcac tgattgaaca gtttcgcaag tcagcttaa                        879

 
<210> 246
<211> 292
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Imine reductase variant from Pseudonocardia spinosispora
 
<400> 246
Met Ser Asp Ser Pro Val Pro Val Thr Val Leu Gly Leu Gly Leu Met 
1               5                   10                  15      
Gly Gln Ala Leu Ala Gly Ala Phe Leu Ala Ala Gly His Pro Thr Thr 
            20                  25                  30          
Val Trp Asn Arg Ser Ala Ala Lys Ala Asp Glu Leu Val Gly Arg Gly 
        35                  40                  45              
Ala Thr Leu Ala Ala Ser Pro Ala Ala Ala Val Ala Ala Gly Ser Leu 
    50                  55                  60                  
Ile Val Val Cys Val Thr Asp His Arg Ala Val Arg Glu Leu Leu Glu 
65                  70                  75                  80  
Pro Leu Gly Asp Ala Leu Pro Gly Arg Val Leu Val Asn Leu Thr Ser 
                85                  90                  95      
Gly Thr Ser Arg Glu Ala Arg Glu Asn Ala Glu His Thr Ala Ala Gln 
            100                 105                 110         
Gly Ala Thr Tyr Leu Asp Gly Val Ile Leu Thr Ile Pro Ser Gly Ile 
        115                 120                 125             
Gly Thr Pro Glu Ala Thr Ile Leu Tyr Ser Gly Pro Arg Ser Thr Phe 
    130                 135                 140                 
Asp Glu His Glu Ser Ala Leu Arg Ala Leu Gly Pro Gly Ala Thr Phe 
145                 150                 155                 160 
Val Gly Glu Asp His Gly Leu Ala Asn Leu Tyr Asp Ala Ala Met Ser 
                165                 170                 175     
Ser Met Met Trp Gly Leu Leu Asn Gly Phe Leu His Gly Thr Ala Leu 
            180                 185                 190         
Leu Gly Thr Ala Gly Val Ser Ala Thr Ser Val Val Pro Met Leu Gly 
        195                 200                 205             
Gln Gly Trp His Ala Met Thr Gly Ile Ile Ala Arg Tyr Ala Asp Gln 
    210                 215                 220                 
Ile Asp Ala Gly Val Tyr Pro Ala Asp Asp Ala Thr Ile Asp Thr Gln 
225                 230                 235                 240 
Arg Ala Ala Met Gln His Leu Val Glu Glu Ser Arg Ala Ala Gly Ile 
                245                 250                 255     
Asn Gly Glu Leu Pro Ala Phe Val Thr Lys Leu Ala Asp Arg Ala Val 
            260                 265                 270         
Ala Glu Gly Arg Gly Gly Asp Ser Tyr Ala Ala Leu Ile Glu Gln Phe 
        275                 280                 285             
Arg Lys Ser Ala                                                 
    290                                                         

 
<210> 247
<211> 879
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Imine reductase variant from Pseudonocardia spinosispora
 
<400> 247
atgagtgatt cacctgtgcc cgtgacagtt ctgggtcttg gtctgatggg tcaggcctta 60
gcgggagcgt ttctggcagc cggtcaccct acgacggttt ggaatcgcag cgcagctaaa 120
gctgatgaac tggttggtcg cggtgcaacc ctggccgcgt ctcccgcggc tgccgtcgca 180
gcgggtagtc tgatcgttgt ttgcgtcaca gatcatcgtg cggtgcgcga actgctggaa 240
ccacttggcg atgcactgcc aggtcgcgtt ctggtcaacc tgacgagcgg tacgtcgcgt 300
gaagcccgtg agaacgcaga acatactgcg gctcagggag ccacctacct ggatggtgtg 360
attctgacca ttccaagcgg cattggcacc ccagaagcta ccattctgta ttcgggaccg 420
cgcagtacct tcgatgaaca tgaaagcgca ctgcgcgcat taggtccggg ggcgaccttt 480
gtaggggaag atcacggact ggctaacctg tatgatgcag caatgtcgag catgatgtgg 540
ggcctgctga atggtttcct gcatggaacc gcactgctgg gaaccgcggg cgtttcggca 600
acctctgtgg ttccgatgct gggtcaaggt tggagggcga tgactggtat aattgcgcgc 660
tacgcagatc agattgatgc cggtgtctat ccagcagacg atgccacgat tgatacccag 720
cgtgctgcaa tgcagcacct ggtcgaggag agccgtgcgg cgggcatcaa tggcgaactt 780
ccggcgtttg tgaccaaact ggccgatcgc gcagtggcag aaggtcgtgg tggtgactct 840
tacgctgcac tgattgaaca gtttcgcaag tcagcttaa                        879

 
<210> 248
<211> 292
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Imine reductase variant from Pseudonocardia spinosispora
 
<400> 248
Met Ser Asp Ser Pro Val Pro Val Thr Val Leu Gly Leu Gly Leu Met 
1               5                   10                  15      
Gly Gln Ala Leu Ala Gly Ala Phe Leu Ala Ala Gly His Pro Thr Thr 
            20                  25                  30          
Val Trp Asn Arg Ser Ala Ala Lys Ala Asp Glu Leu Val Gly Arg Gly 
        35                  40                  45              
Ala Thr Leu Ala Ala Ser Pro Ala Ala Ala Val Ala Ala Gly Ser Leu 
    50                  55                  60                  
Ile Val Val Cys Val Thr Asp His Arg Ala Val Arg Glu Leu Leu Glu 
65                  70                  75                  80  
Pro Leu Gly Asp Ala Leu Pro Gly Arg Val Leu Val Asn Leu Thr Ser 
                85                  90                  95      
Gly Thr Ser Arg Glu Ala Arg Glu Asn Ala Glu His Thr Ala Ala Gln 
            100                 105                 110         
Gly Ala Thr Tyr Leu Asp Gly Val Ile Leu Thr Ile Pro Ser Gly Ile 
        115                 120                 125             
Gly Thr Pro Glu Ala Thr Ile Leu Tyr Ser Gly Pro Arg Ser Thr Phe 
    130                 135                 140                 
Asp Glu His Glu Ser Ala Leu Arg Ala Leu Gly Pro Gly Ala Thr Phe 
145                 150                 155                 160 
Val Gly Glu Asp His Gly Leu Ala Asn Leu Tyr Asp Ala Ala Met Ser 
                165                 170                 175     
Ser Met Met Trp Gly Leu Leu Asn Gly Phe Leu His Gly Thr Ala Leu 
            180                 185                 190         
Leu Gly Thr Ala Gly Val Ser Ala Thr Ser Val Val Pro Met Leu Gly 
        195                 200                 205             
Gln Gly Trp Arg Ala Met Thr Gly Ile Ile Ala Arg Tyr Ala Asp Gln 
    210                 215                 220                 
Ile Asp Ala Gly Val Tyr Pro Ala Asp Asp Ala Thr Ile Asp Thr Gln 
225                 230                 235                 240 
Arg Ala Ala Met Gln His Leu Val Glu Glu Ser Arg Ala Ala Gly Ile 
                245                 250                 255     
Asn Gly Glu Leu Pro Ala Phe Val Thr Lys Leu Ala Asp Arg Ala Val 
            260                 265                 270         
Ala Glu Gly Arg Gly Gly Asp Ser Tyr Ala Ala Leu Ile Glu Gln Phe 
        275                 280                 285             
Arg Lys Ser Ala                                                 
    290                                                         

 
<210> 249
<211> 879
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Imine reductase variant from Pseudonocardia spinosispora
 
<400> 249
atgagtgatt cacctgtgcc cgtgacagtt ctgggtcttg gtctgatggg tcaggcctta 60
gcgggagcgt ttctggcagc cggtcaccct acgacggttt ggaatcgcag cgcagctaaa 120
gctgatgaac tggttggtcg cggtgcaacc ctggccgcgt ctcccgcggc tgccgtcgca 180
gcgggtagtc tgatcgttgt ttgcgtcaca gatcatcgtg cggtgcgcga actgctggaa 240
ccacttggcg atgcactgcc aggtcgcgtt ctggtcaacc tgacgagcgg tacgtcgcgt 300
gaagcccgtg agaacgcaga acatactgcg gctcagggag ccacctacct ggatggtgtg 360
attctgacca ttccaagcgg cattggcacc ccagaagcta ccattctgta ttcgggaccg 420
cgcagtacct tcgatgaaca tgaaagcgca ctgcgcgcat taggtccggg ggcgaccttt 480
gtaggggaag atcacggact ggctaacctg tatgatgcag caatgtcgag catgatgtgg 540
ggcctgctga atggtttcct gcatggaacc gcactgctgg gaaccgcggg cgtttcggca 600
acctctgtgg ttccgatgct gggtcaaggt tggaaagcga tgactggtat aattgcgcgc 660
tacgcagatc agattgatgc cggtgtctat ccagcagacg atgccacgat tgatacccag 720
cgtgctgcaa tgcagcacct ggtcgaggag agccgtgcgg cgggcatcaa tggcgaactt 780
ccggcgtttg tgaccaaact ggccgatcgc gcagtggcag aaggtcgtgg tggtgactct 840
tacgctgcac tgattgaaca gtttcgcaag tcagcttaa                        879

 
<210> 250
<211> 292
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Imine reductase variant from Pseudonocardia spinosispora
 
<400> 250
Met Ser Asp Ser Pro Val Pro Val Thr Val Leu Gly Leu Gly Leu Met 
1               5                   10                  15      
Gly Gln Ala Leu Ala Gly Ala Phe Leu Ala Ala Gly His Pro Thr Thr 
            20                  25                  30          
Val Trp Asn Arg Ser Ala Ala Lys Ala Asp Glu Leu Val Gly Arg Gly 
        35                  40                  45              
Ala Thr Leu Ala Ala Ser Pro Ala Ala Ala Val Ala Ala Gly Ser Leu 
    50                  55                  60                  
Ile Val Val Cys Val Thr Asp His Arg Ala Val Arg Glu Leu Leu Glu 
65                  70                  75                  80  
Pro Leu Gly Asp Ala Leu Pro Gly Arg Val Leu Val Asn Leu Thr Ser 
                85                  90                  95      
Gly Thr Ser Arg Glu Ala Arg Glu Asn Ala Glu His Thr Ala Ala Gln 
            100                 105                 110         
Gly Ala Thr Tyr Leu Asp Gly Val Ile Leu Thr Ile Pro Ser Gly Ile 
        115                 120                 125             
Gly Thr Pro Glu Ala Thr Ile Leu Tyr Ser Gly Pro Arg Ser Thr Phe 
    130                 135                 140                 
Asp Glu His Glu Ser Ala Leu Arg Ala Leu Gly Pro Gly Ala Thr Phe 
145                 150                 155                 160 
Val Gly Glu Asp His Gly Leu Ala Asn Leu Tyr Asp Ala Ala Met Ser 
                165                 170                 175     
Ser Met Met Trp Gly Leu Leu Asn Gly Phe Leu His Gly Thr Ala Leu 
            180                 185                 190         
Leu Gly Thr Ala Gly Val Ser Ala Thr Ser Val Val Pro Met Leu Gly 
        195                 200                 205             
Gln Gly Trp Lys Ala Met Thr Gly Ile Ile Ala Arg Tyr Ala Asp Gln 
    210                 215                 220                 
Ile Asp Ala Gly Val Tyr Pro Ala Asp Asp Ala Thr Ile Asp Thr Gln 
225                 230                 235                 240 
Arg Ala Ala Met Gln His Leu Val Glu Glu Ser Arg Ala Ala Gly Ile 
                245                 250                 255     
Asn Gly Glu Leu Pro Ala Phe Val Thr Lys Leu Ala Asp Arg Ala Val 
            260                 265                 270         
Ala Glu Gly Arg Gly Gly Asp Ser Tyr Ala Ala Leu Ile Glu Gln Phe 
        275                 280                 285             
Arg Lys Ser Ala                                                 
    290                                                         

 
<210> 251
<211> 879
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Imine reductase variant from Pseudonocardia spinosispora
 
<400> 251
atgagtgatt cacctgtgcc cgtgacagtt ctgggtcttg gtctgatggg tcaggcctta 60
gcgggagcgt ttctggcagc cggtcaccct acgacggttt ggaatcgcag cgcagctaaa 120
gctgatgaac tggttggtcg cggtgcaacc ctggccgcgt ctcccgcggc tgccgtcgca 180
gcgggtagtc tgatcgttgt ttgcgtcaca gatcatcgtg cggtgcgcga actgctggaa 240
ccacttggcg atgcactgcc aggtcgcgtt ctggtcaacc tgacgagcgg tacgtcgcgt 300
gaagcccgtg agaacgcaga acatactgcg gctcagggag ccacctacct ggatggtgtg 360
attctgacca ttccaagcgg cattggcacc ccagaagcta ccattctgta ttcgggaccg 420
cgcagtacct tcgatgaaca tgaaagcgca ctgcgcgcat taggtccggg ggcgaccttt 480
gtaggggaag atcacggact ggctaacctg tatgatgcag caatgtcgag catgatgtgg 540
ggcctgctga atggtttcct gcatggaacc gcactgctgg gaaccgcggg cgtttcggca 600
acctctgtgg ttccgatgct gggtagtggt tgggaagcga tgactggtat aattgcgcgc 660
tacgcagatc agattgatgc cggtgtctat ccagcagacg atgccacgat tgatacccag 720
cgtgctgcaa tgcagcacct ggtcgaggag agccgtgcgg cgggcatcaa tggcgaactt 780
ccggcgtttg tgaccaaact ggccgatcgc gcagtggcag aaggtcgtgg tggtgactct 840
tacgctgcac tgattgaaca gtttcgcaag tcagcttaa                        879

 
<210> 252
<211> 292
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Imine reductase variant from Pseudonocardia spinosispora
 
<400> 252
Met Ser Asp Ser Pro Val Pro Val Thr Val Leu Gly Leu Gly Leu Met 
1               5                   10                  15      
Gly Gln Ala Leu Ala Gly Ala Phe Leu Ala Ala Gly His Pro Thr Thr 
            20                  25                  30          
Val Trp Asn Arg Ser Ala Ala Lys Ala Asp Glu Leu Val Gly Arg Gly 
        35                  40                  45              
Ala Thr Leu Ala Ala Ser Pro Ala Ala Ala Val Ala Ala Gly Ser Leu 
    50                  55                  60                  
Ile Val Val Cys Val Thr Asp His Arg Ala Val Arg Glu Leu Leu Glu 
65                  70                  75                  80  
Pro Leu Gly Asp Ala Leu Pro Gly Arg Val Leu Val Asn Leu Thr Ser 
                85                  90                  95      
Gly Thr Ser Arg Glu Ala Arg Glu Asn Ala Glu His Thr Ala Ala Gln 
            100                 105                 110         
Gly Ala Thr Tyr Leu Asp Gly Val Ile Leu Thr Ile Pro Ser Gly Ile 
        115                 120                 125             
Gly Thr Pro Glu Ala Thr Ile Leu Tyr Ser Gly Pro Arg Ser Thr Phe 
    130                 135                 140                 
Asp Glu His Glu Ser Ala Leu Arg Ala Leu Gly Pro Gly Ala Thr Phe 
145                 150                 155                 160 
Val Gly Glu Asp His Gly Leu Ala Asn Leu Tyr Asp Ala Ala Met Ser 
                165                 170                 175     
Ser Met Met Trp Gly Leu Leu Asn Gly Phe Leu His Gly Thr Ala Leu 
            180                 185                 190         
Leu Gly Thr Ala Gly Val Ser Ala Thr Ser Val Val Pro Met Leu Gly 
        195                 200                 205             
Ser Gly Trp Glu Ala Met Thr Gly Ile Ile Ala Arg Tyr Ala Asp Gln 
    210                 215                 220                 
Ile Asp Ala Gly Val Tyr Pro Ala Asp Asp Ala Thr Ile Asp Thr Gln 
225                 230                 235                 240 
Arg Ala Ala Met Gln His Leu Val Glu Glu Ser Arg Ala Ala Gly Ile 
                245                 250                 255     
Asn Gly Glu Leu Pro Ala Phe Val Thr Lys Leu Ala Asp Arg Ala Val 
            260                 265                 270         
Ala Glu Gly Arg Gly Gly Asp Ser Tyr Ala Ala Leu Ile Glu Gln Phe 
        275                 280                 285             
Arg Lys Ser Ala                                                 
    290                                                         

 
<210> 253
<211> 879
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Imine reductase variant from Pseudonocardia spinosispora
 
<400> 253
atgagtgatt cacctgtgcc cgtgacagtt ctgggtcttg gtctgatggg tcaggcctta 60
gcgggagcgt ttctggcagc cggtcaccct acgacggttt ggaatcgcag cgcagctaaa 120
gctgatgaac tggttggtcg cggtgcaacc ctggccgcgt ctcccgcggc tgccgtcgca 180
gcgggtagtc tgatcgttgt ttgcgtcaca gatcatcgtg cggtgcgcga actgctggaa 240
ccacttggcg atgcactgcc aggtcgcgtt ctggtcaacc tgacgagcgg tacgtcgcgt 300
gaagcccgtg agaacgcaga acatactgcg gctcagggag ccacctacct ggatggtgtg 360
attctgacca ttccaagcgg cattggcacc ccagaagcta ccattctgta ttcgggaccg 420
cgcagtacct tcgatgaaca tgaaagcgca ctgcgcgcat taggtccggg ggcgaccttt 480
gtaggggaag atcacggact ggctaacctg tatgatgcag caatgtcgag catgatgtgg 540
ggcctgctga atggtttcct gcatggaacc gcactgctgg gaaccgcggg cgtttcggca 600
acctctgtgg ttccgatgct gggtcaaggt tgggaagcga tgactcgtat aattgcgcgc 660
tacgcagatc agattgatgc cggtgtctat ccagcagacg atgccacgat tgatacccag 720
cgtgctgcaa tgcagcacct ggtcgaggag agccgtgcgg cgggcatcaa tggcgaactt 780
ccggcgtttg tgaccaaact ggccgatcgc gcagtggcag aaggtcgtgg tggtgactct 840
tacgctgcac tgattgaaca gtttcgcaag tcagcttaa                        879

 
<210> 254
<211> 292
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Imine reductase variant from Pseudonocardia spinosispora
 
<400> 254
Met Ser Asp Ser Pro Val Pro Val Thr Val Leu Gly Leu Gly Leu Met 
1               5                   10                  15      
Gly Gln Ala Leu Ala Gly Ala Phe Leu Ala Ala Gly His Pro Thr Thr 
            20                  25                  30          
Val Trp Asn Arg Ser Ala Ala Lys Ala Asp Glu Leu Val Gly Arg Gly 
        35                  40                  45              
Ala Thr Leu Ala Ala Ser Pro Ala Ala Ala Val Ala Ala Gly Ser Leu 
    50                  55                  60                  
Ile Val Val Cys Val Thr Asp His Arg Ala Val Arg Glu Leu Leu Glu 
65                  70                  75                  80  
Pro Leu Gly Asp Ala Leu Pro Gly Arg Val Leu Val Asn Leu Thr Ser 
                85                  90                  95      
Gly Thr Ser Arg Glu Ala Arg Glu Asn Ala Glu His Thr Ala Ala Gln 
            100                 105                 110         
Gly Ala Thr Tyr Leu Asp Gly Val Ile Leu Thr Ile Pro Ser Gly Ile 
        115                 120                 125             
Gly Thr Pro Glu Ala Thr Ile Leu Tyr Ser Gly Pro Arg Ser Thr Phe 
    130                 135                 140                 
Asp Glu His Glu Ser Ala Leu Arg Ala Leu Gly Pro Gly Ala Thr Phe 
145                 150                 155                 160 
Val Gly Glu Asp His Gly Leu Ala Asn Leu Tyr Asp Ala Ala Met Ser 
                165                 170                 175     
Ser Met Met Trp Gly Leu Leu Asn Gly Phe Leu His Gly Thr Ala Leu 
            180                 185                 190         
Leu Gly Thr Ala Gly Val Ser Ala Thr Ser Val Val Pro Met Leu Gly 
        195                 200                 205             
Gln Gly Trp Glu Ala Met Thr Arg Ile Ile Ala Arg Tyr Ala Asp Gln 
    210                 215                 220                 
Ile Asp Ala Gly Val Tyr Pro Ala Asp Asp Ala Thr Ile Asp Thr Gln 
225                 230                 235                 240 
Arg Ala Ala Met Gln His Leu Val Glu Glu Ser Arg Ala Ala Gly Ile 
                245                 250                 255     
Asn Gly Glu Leu Pro Ala Phe Val Thr Lys Leu Ala Asp Arg Ala Val 
            260                 265                 270         
Ala Glu Gly Arg Gly Gly Asp Ser Tyr Ala Ala Leu Ile Glu Gln Phe 
        275                 280                 285             
Arg Lys Ser Ala                                                 
    290                                                         

 
<210> 255
<211> 879
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Imine reductase variant from Pseudonocardia spinosispora
 
<400> 255
atgagtgatt cacctgtgcc cgtgacagtt ctgggtcttg gtctgatggg tcaggcctta 60
gcgggagcgt ttctggcagc cggtcaccct acgacggttt ggaatcgcag cgcagctaaa 120
gctgatgaac tggttggtcg cggtgcaacc ctggccgcgt ctcccgcggc tgccgtcgca 180
gcgggtagtc tgatcgttgt ttgcgtcaca gatcatcgtg cggtgcgcga actgctggaa 240
ccacttggcg atgcactgcc aggtcgcgtt ctggtcaacc tgacgagcgg tacgtcgcgt 300
gaagcccgtg agaacgcaga acatactgcg gctcagggag ccacctacct ggatggtgtg 360
attctgacca ttccaagcgg cattggcacc ccagaagcta ccattctgta ttcgggaccg 420
cgcagtacct tcgatgaaca tgaaagcgca ctgcgcgcat taggtccggg ggcgaccttt 480
gtaggggaag atcacggact ggctaacctg tatgatgcag caatgtcgag catgatgtgg 540
ggcctgctga atggtttcct gcatggaacc gcactgctgg gaaccgcggg cgtttcggca 600
acctctgtgg ttccgcttct gggtcaaggt tgggaagcga tgactggtat aattgcgcgc 660
tacgcagatc agattgatgc cggtgtctat ccagcagacg atgccacgat tgatacccag 720
cgtgctgcaa tgcagcacct ggtcgaggag agccgtgcgg cgggcatcaa tggcgaactt 780
ccggcgtttg tgaccaaact ggccgatcgc gcagtggcag aaggtcgtgg tggtgactct 840
tacgctgcac tgattgaaca gtttcgcaag tcagcttaa                        879

 
<210> 256
<211> 292
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Imine reductase variant from Pseudonocardia spinosispora
 
<400> 256
Met Ser Asp Ser Pro Val Pro Val Thr Val Leu Gly Leu Gly Leu Met 
1               5                   10                  15      
Gly Gln Ala Leu Ala Gly Ala Phe Leu Ala Ala Gly His Pro Thr Thr 
            20                  25                  30          
Val Trp Asn Arg Ser Ala Ala Lys Ala Asp Glu Leu Val Gly Arg Gly 
        35                  40                  45              
Ala Thr Leu Ala Ala Ser Pro Ala Ala Ala Val Ala Ala Gly Ser Leu 
    50                  55                  60                  
Ile Val Val Cys Val Thr Asp His Arg Ala Val Arg Glu Leu Leu Glu 
65                  70                  75                  80  
Pro Leu Gly Asp Ala Leu Pro Gly Arg Val Leu Val Asn Leu Thr Ser 
                85                  90                  95      
Gly Thr Ser Arg Glu Ala Arg Glu Asn Ala Glu His Thr Ala Ala Gln 
            100                 105                 110         
Gly Ala Thr Tyr Leu Asp Gly Val Ile Leu Thr Ile Pro Ser Gly Ile 
        115                 120                 125             
Gly Thr Pro Glu Ala Thr Ile Leu Tyr Ser Gly Pro Arg Ser Thr Phe 
    130                 135                 140                 
Asp Glu His Glu Ser Ala Leu Arg Ala Leu Gly Pro Gly Ala Thr Phe 
145                 150                 155                 160 
Val Gly Glu Asp His Gly Leu Ala Asn Leu Tyr Asp Ala Ala Met Ser 
                165                 170                 175     
Ser Met Met Trp Gly Leu Leu Asn Gly Phe Leu His Gly Thr Ala Leu 
            180                 185                 190         
Leu Gly Thr Ala Gly Val Ser Ala Thr Ser Val Val Pro Leu Leu Gly 
        195                 200                 205             
Gln Gly Trp Glu Ala Met Thr Gly Ile Ile Ala Arg Tyr Ala Asp Gln 
    210                 215                 220                 
Ile Asp Ala Gly Val Tyr Pro Ala Asp Asp Ala Thr Ile Asp Thr Gln 
225                 230                 235                 240 
Arg Ala Ala Met Gln His Leu Val Glu Glu Ser Arg Ala Ala Gly Ile 
                245                 250                 255     
Asn Gly Glu Leu Pro Ala Phe Val Thr Lys Leu Ala Asp Arg Ala Val 
            260                 265                 270         
Ala Glu Gly Arg Gly Gly Asp Ser Tyr Ala Ala Leu Ile Glu Gln Phe 
        275                 280                 285             
Arg Lys Ser Ala                                                 
    290                                                         

 
<210> 257
<211> 879
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Imine reductase variant from Pseudonocardia spinosispora
 
<400> 257
atgagtgatt cacctgtgcc cgtgacagtt ctgggtcttg gtctgatggg tcaggcctta 60
gcgggagcgt ttctggcagc cggtcaccct acgacggttt ggaatcgcag cgcagctaaa 120
gctgatgaac tggttggtcg cggtgcaacc ctggccgcgt ctcccgcggc tgccgtcgca 180
gcgggtagtc tgatcgttgt ttgcgtcaca gatcatcgtg cggtgcgcga actgctggaa 240
ccacttggcg atgcactgcc aggtcgcgtt ctggtcaacc tgacgagcgg tacgtcgcgt 300
gaagcccgtg agaacgcaga acatactgcg gctcagggag ccacctacct ggatggtgtg 360
attctgacca ttccaagcgg cattggcacc ccagaagctc ttattctgta ttcgggaccg 420
cgcagtacct tcgatgaaca tgaaagcgca ctgcgcgcat taggtccggg ggcgaccttt 480
gtaggggaag atcacggact ggctaacctg tatgatgcag caatgtcgag catgatgtgg 540
ggcctgctga atggtttcct gcatggaacc gcactgctgg gaaccgcggg cgtttcggca 600
acctctgtgg ttccgatgct gggtcaaggt tgggaagcga tgactggtat aattgcgcgc 660
tacgcagatc agattgatgc cggtgtctat ccagcagacg atgccacgat tgatacccag 720
cgtgctgcaa tgcagcacct ggtcgaggag agccgtgcgg cgggcatcaa tggcgaactt 780
ccggcgtttg tgaccaaact ggccgatcgc gcagtggcag aaggtcgtgg tggtgactct 840
tacgctgcac tgattgaaca gtttcgcaag tcagcttaa                        879

 
<210> 258
<211> 292
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Imine reductase variant from Pseudonocardia spinosispora
 
<400> 258
Met Ser Asp Ser Pro Val Pro Val Thr Val Leu Gly Leu Gly Leu Met 
1               5                   10                  15      
Gly Gln Ala Leu Ala Gly Ala Phe Leu Ala Ala Gly His Pro Thr Thr 
            20                  25                  30          
Val Trp Asn Arg Ser Ala Ala Lys Ala Asp Glu Leu Val Gly Arg Gly 
        35                  40                  45              
Ala Thr Leu Ala Ala Ser Pro Ala Ala Ala Val Ala Ala Gly Ser Leu 
    50                  55                  60                  
Ile Val Val Cys Val Thr Asp His Arg Ala Val Arg Glu Leu Leu Glu 
65                  70                  75                  80  
Pro Leu Gly Asp Ala Leu Pro Gly Arg Val Leu Val Asn Leu Thr Ser 
                85                  90                  95      
Gly Thr Ser Arg Glu Ala Arg Glu Asn Ala Glu His Thr Ala Ala Gln 
            100                 105                 110         
Gly Ala Thr Tyr Leu Asp Gly Val Ile Leu Thr Ile Pro Ser Gly Ile 
        115                 120                 125             
Gly Thr Pro Glu Ala Leu Ile Leu Tyr Ser Gly Pro Arg Ser Thr Phe 
    130                 135                 140                 
Asp Glu His Glu Ser Ala Leu Arg Ala Leu Gly Pro Gly Ala Thr Phe 
145                 150                 155                 160 
Val Gly Glu Asp His Gly Leu Ala Asn Leu Tyr Asp Ala Ala Met Ser 
                165                 170                 175     
Ser Met Met Trp Gly Leu Leu Asn Gly Phe Leu His Gly Thr Ala Leu 
            180                 185                 190         
Leu Gly Thr Ala Gly Val Ser Ala Thr Ser Val Val Pro Met Leu Gly 
        195                 200                 205             
Gln Gly Trp Glu Ala Met Thr Gly Ile Ile Ala Arg Tyr Ala Asp Gln 
    210                 215                 220                 
Ile Asp Ala Gly Val Tyr Pro Ala Asp Asp Ala Thr Ile Asp Thr Gln 
225                 230                 235                 240 
Arg Ala Ala Met Gln His Leu Val Glu Glu Ser Arg Ala Ala Gly Ile 
                245                 250                 255     
Asn Gly Glu Leu Pro Ala Phe Val Thr Lys Leu Ala Asp Arg Ala Val 
            260                 265                 270         
Ala Glu Gly Arg Gly Gly Asp Ser Tyr Ala Ala Leu Ile Glu Gln Phe 
        275                 280                 285             
Arg Lys Ser Ala                                                 
    290                                                         

 
<210> 259
<211> 879
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Imine reductase variant from Pseudonocardia spinosispora
 
<400> 259
atgagtgatt cacctgtgcc cgtgacagtt ctgggtcttg gtctgatggg tcaggcctta 60
gcgggagcgt ttctggcagc cggtcaccct acgacggttt ggaatcgcag cgcagctaaa 120
gctgatgaac tggttggtcg cggtgcaacc ctggccgcgt ctcccgcggc tgccgtcgca 180
gcgggtagtc tgatcgttgt ttgcgtcaca gatcatcgtg cggtgcgcga actgctggaa 240
ccacttggcg atgcactgcc aggtcgcgtt ctggtcaacc tgacgagcgg tacgtcgcgt 300
gaagcccgtg agaacgcaga acatactgcg gctcagggag ccacctacct ggatggtgtg 360
attctgacca ttccaagcgg cattggccgt ccagaagcta ccattctgta ttcgggaccg 420
cgcagtacct tcgatgaaca tgaaagcgca ctgcgcgcat taggtccggg ggcgaccttt 480
gtaggggaag atcacggact ggctaacctg tatgatgcag caatgtcgag catgatgtgg 540
ggcctgctga atggtttcct gcatggaacc gcactgctgg gaaccgcggg cgtttcggca 600
acctctgtgg ttccgatgct gggtcaaggt tgggaagcga tgactggtat aattgcgcgc 660
tacgcagatc agattgatgc cggtgtctat ccagcagacg atgccacgat tgatacccag 720
cgtgctgcaa tgcagcacct ggtcgaggag agccgtgcgg cgggcatcaa tggcgaactt 780
ccggcgtttg tgaccaaact ggccgatcgc gcagtggcag aaggtcgtgg tggtgactct 840
tacgctgcac tgattgaaca gtttcgcaag tcagcttaa                        879

 
<210> 260
<211> 292
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Imine reductase variant from Pseudonocardia spinosispora
 
<400> 260
Met Ser Asp Ser Pro Val Pro Val Thr Val Leu Gly Leu Gly Leu Met 
1               5                   10                  15      
Gly Gln Ala Leu Ala Gly Ala Phe Leu Ala Ala Gly His Pro Thr Thr 
            20                  25                  30          
Val Trp Asn Arg Ser Ala Ala Lys Ala Asp Glu Leu Val Gly Arg Gly 
        35                  40                  45              
Ala Thr Leu Ala Ala Ser Pro Ala Ala Ala Val Ala Ala Gly Ser Leu 
    50                  55                  60                  
Ile Val Val Cys Val Thr Asp His Arg Ala Val Arg Glu Leu Leu Glu 
65                  70                  75                  80  
Pro Leu Gly Asp Ala Leu Pro Gly Arg Val Leu Val Asn Leu Thr Ser 
                85                  90                  95      
Gly Thr Ser Arg Glu Ala Arg Glu Asn Ala Glu His Thr Ala Ala Gln 
            100                 105                 110         
Gly Ala Thr Tyr Leu Asp Gly Val Ile Leu Thr Ile Pro Ser Gly Ile 
        115                 120                 125             
Gly Arg Pro Glu Ala Thr Ile Leu Tyr Ser Gly Pro Arg Ser Thr Phe 
    130                 135                 140                 
Asp Glu His Glu Ser Ala Leu Arg Ala Leu Gly Pro Gly Ala Thr Phe 
145                 150                 155                 160 
Val Gly Glu Asp His Gly Leu Ala Asn Leu Tyr Asp Ala Ala Met Ser 
                165                 170                 175     
Ser Met Met Trp Gly Leu Leu Asn Gly Phe Leu His Gly Thr Ala Leu 
            180                 185                 190         
Leu Gly Thr Ala Gly Val Ser Ala Thr Ser Val Val Pro Met Leu Gly 
        195                 200                 205             
Gln Gly Trp Glu Ala Met Thr Gly Ile Ile Ala Arg Tyr Ala Asp Gln 
    210                 215                 220                 
Ile Asp Ala Gly Val Tyr Pro Ala Asp Asp Ala Thr Ile Asp Thr Gln 
225                 230                 235                 240 
Arg Ala Ala Met Gln His Leu Val Glu Glu Ser Arg Ala Ala Gly Ile 
                245                 250                 255     
Asn Gly Glu Leu Pro Ala Phe Val Thr Lys Leu Ala Asp Arg Ala Val 
            260                 265                 270         
Ala Glu Gly Arg Gly Gly Asp Ser Tyr Ala Ala Leu Ile Glu Gln Phe 
        275                 280                 285             
Arg Lys Ser Ala                                                 
    290                                                         

 
<210> 261
<211> 879
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Imine reductase variant from Pseudonocardia spinosispora
 
<400> 261
atgagtgatt cacctgtgcc cgtgacagtt ctgggtcttg gtctgatggg tcaggcctta 60
gcgggagcgt ttctggcagc cggtcaccct acgacggttt ggaatcgcag cgcagctaaa 120
gctgatgaac tggttggtcg cggtgcaacc ctggccgcgt ctcccgcggc tgccgtcgca 180
gcgggtagtc tgatcgttgt ttgcgtcaca gatcatcgtg cggtgcgcga actgctggaa 240
ccacttggcg atgcactgcc aggtcgcgtt ctggtcaacc tgacgagcgg tacgtcgcgt 300
gaagcccgtg agaacgcaga acatactgcg gctcagggag ccacctacct ggatggtgtg 360
attctgacca ttccaagcgg cattggcacc ccagaagcta ccattctgta ttcgggaccg 420
cgcagtacct tcgatgaaca tgaaagcgca ctgcgcgcat taggtccggg ggcgaccttt 480
gtaggggaag atcacggact ggctaacctg tatgatgcag caatgtcgag catgatgtgg 540
ggcctgctga atggtttcct gcatggaacc gcactgctgg gaaccgcggg cgtttcggca 600
acctctgtgg ttccgatgct gggtcaaggt tgggaagcga tgactaatat aattgcgcgc 660
tacgcagatc agattgatgc cggtgtctat ccagcagacg atgccacgat tgatacccag 720
cgtgctgcaa tgcagcacct ggtcgaggag agccgtgcgg cgggcatcaa tggcgaactt 780
ccggcgtttg tgaccaaact ggccgatcgc gcagtggcag aaggtcgtgg tggtgactct 840
tacgctgcac tgattgaaca gtttcgcaag tcagcttaa                        879

 
<210> 262
<211> 292
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Imine reductase variant from Pseudonocardia spinosispora
 
<400> 262
Met Ser Asp Ser Pro Val Pro Val Thr Val Leu Gly Leu Gly Leu Met 
1               5                   10                  15      
Gly Gln Ala Leu Ala Gly Ala Phe Leu Ala Ala Gly His Pro Thr Thr 
            20                  25                  30          
Val Trp Asn Arg Ser Ala Ala Lys Ala Asp Glu Leu Val Gly Arg Gly 
        35                  40                  45              
Ala Thr Leu Ala Ala Ser Pro Ala Ala Ala Val Ala Ala Gly Ser Leu 
    50                  55                  60                  
Ile Val Val Cys Val Thr Asp His Arg Ala Val Arg Glu Leu Leu Glu 
65                  70                  75                  80  
Pro Leu Gly Asp Ala Leu Pro Gly Arg Val Leu Val Asn Leu Thr Ser 
                85                  90                  95      
Gly Thr Ser Arg Glu Ala Arg Glu Asn Ala Glu His Thr Ala Ala Gln 
            100                 105                 110         
Gly Ala Thr Tyr Leu Asp Gly Val Ile Leu Thr Ile Pro Ser Gly Ile 
        115                 120                 125             
Gly Thr Pro Glu Ala Thr Ile Leu Tyr Ser Gly Pro Arg Ser Thr Phe 
    130                 135                 140                 
Asp Glu His Glu Ser Ala Leu Arg Ala Leu Gly Pro Gly Ala Thr Phe 
145                 150                 155                 160 
Val Gly Glu Asp His Gly Leu Ala Asn Leu Tyr Asp Ala Ala Met Ser 
                165                 170                 175     
Ser Met Met Trp Gly Leu Leu Asn Gly Phe Leu His Gly Thr Ala Leu 
            180                 185                 190         
Leu Gly Thr Ala Gly Val Ser Ala Thr Ser Val Val Pro Met Leu Gly 
        195                 200                 205             
Gln Gly Trp Glu Ala Met Thr Asn Ile Ile Ala Arg Tyr Ala Asp Gln 
    210                 215                 220                 
Ile Asp Ala Gly Val Tyr Pro Ala Asp Asp Ala Thr Ile Asp Thr Gln 
225                 230                 235                 240 
Arg Ala Ala Met Gln His Leu Val Glu Glu Ser Arg Ala Ala Gly Ile 
                245                 250                 255     
Asn Gly Glu Leu Pro Ala Phe Val Thr Lys Leu Ala Asp Arg Ala Val 
            260                 265                 270         
Ala Glu Gly Arg Gly Gly Asp Ser Tyr Ala Ala Leu Ile Glu Gln Phe 
        275                 280                 285             
Arg Lys Ser Ala                                                 
    290                                                         

 
<210> 263
<211> 879
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Imine reductase variant from Pseudonocardia spinosispora
 
<400> 263
atgagtgatt cacctgtgcc cgtgacagtt ctgggtcttg gtctgatggg tcaggcctta 60
gcgggagcgt ttctggcagc cggtcaccct acgacggttt ggaatcgcag cgcagctaaa 120
gctgatgaac tgattggtcg cggtgcaacc ctggccgcgt ctcccgcggc tgccgtcgca 180
gcgggtagtc tgatcgttgt ttgcgtcaca gatcatcgtg cggtgcgcga actgctggaa 240
ccacttggcg atgcactgcc aggtcgcgtt ctggtcaacc tgacgagcgg tacgtcgcgt 300
gaagcccgtg agaacgcaga acatactgcg gctcagggag ccacctacct ggatggtgtg 360
attctgacca ttccaagcgg cattggcacc ccagaagcta ccattctgta ttcgggaccg 420
cgcagtacct tcgatgaaca tgaaagcgca ctgcgcgcat taggtccggg ggcgaccttt 480
gtaggggaag atcacggact ggctaacctg tatgatgcag caatgtcgag catgatgtgg 540
ggcctgctga atggtttcct gcatggaacc gcactgctgg gaaccgcggg cgtttcggca 600
acctctgtgg ttccgatgct gggtcaaggt tgggaagcga tgactggtat aattgcgcgc 660
tacgcagatc agattgatgc cggtgtctat ccagcagacg atgccacgat tgatacccag 720
cgtgctgcaa tgcagcacct ggtcgaggag agccgtgcgg cgggcatcaa tggcgaactt 780
ccggcgtttg tgaccaaact ggccgatcgc gcagtggcag aaggtcgtgg tggtgactct 840
tacgctgcac tgattgaaca gtttcgcaag tcagcttaa                        879

 
<210> 264
<211> 292
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Imine reductase variant from Pseudonocardia spinosispora
 
<400> 264
Met Ser Asp Ser Pro Val Pro Val Thr Val Leu Gly Leu Gly Leu Met 
1               5                   10                  15      
Gly Gln Ala Leu Ala Gly Ala Phe Leu Ala Ala Gly His Pro Thr Thr 
            20                  25                  30          
Val Trp Asn Arg Ser Ala Ala Lys Ala Asp Glu Leu Ile Gly Arg Gly 
        35                  40                  45              
Ala Thr Leu Ala Ala Ser Pro Ala Ala Ala Val Ala Ala Gly Ser Leu 
    50                  55                  60                  
Ile Val Val Cys Val Thr Asp His Arg Ala Val Arg Glu Leu Leu Glu 
65                  70                  75                  80  
Pro Leu Gly Asp Ala Leu Pro Gly Arg Val Leu Val Asn Leu Thr Ser 
                85                  90                  95      
Gly Thr Ser Arg Glu Ala Arg Glu Asn Ala Glu His Thr Ala Ala Gln 
            100                 105                 110         
Gly Ala Thr Tyr Leu Asp Gly Val Ile Leu Thr Ile Pro Ser Gly Ile 
        115                 120                 125             
Gly Thr Pro Glu Ala Thr Ile Leu Tyr Ser Gly Pro Arg Ser Thr Phe 
    130                 135                 140                 
Asp Glu His Glu Ser Ala Leu Arg Ala Leu Gly Pro Gly Ala Thr Phe 
145                 150                 155                 160 
Val Gly Glu Asp His Gly Leu Ala Asn Leu Tyr Asp Ala Ala Met Ser 
                165                 170                 175     
Ser Met Met Trp Gly Leu Leu Asn Gly Phe Leu His Gly Thr Ala Leu 
            180                 185                 190         
Leu Gly Thr Ala Gly Val Ser Ala Thr Ser Val Val Pro Met Leu Gly 
        195                 200                 205             
Gln Gly Trp Glu Ala Met Thr Gly Ile Ile Ala Arg Tyr Ala Asp Gln 
    210                 215                 220                 
Ile Asp Ala Gly Val Tyr Pro Ala Asp Asp Ala Thr Ile Asp Thr Gln 
225                 230                 235                 240 
Arg Ala Ala Met Gln His Leu Val Glu Glu Ser Arg Ala Ala Gly Ile 
                245                 250                 255     
Asn Gly Glu Leu Pro Ala Phe Val Thr Lys Leu Ala Asp Arg Ala Val 
            260                 265                 270         
Ala Glu Gly Arg Gly Gly Asp Ser Tyr Ala Ala Leu Ile Glu Gln Phe 
        275                 280                 285             
Arg Lys Ser Ala                                                 
    290                                                         

 
<210> 265
<211> 879
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Imine reductase variant from Pseudonocardia spinosispora
 
<400> 265
atgagtgatt cacctgtgcc cgtgacagtt ctgggtcttg gtctgatggg tcaggcctta 60
gcgggagcgt ttctggcagc cggtcaccct acgacggttt ggaatcgcag cgcagctaaa 120
gctgatgaac tggttggtcg cggtgcaacc ctggccgcgt ctcccgcggc tgccgtcgca 180
gcgggtagtc tgatcgttgt ttgcgtcaca gatcatcgtg cggtgcgcga actgctggaa 240
ccacttggcg atgcactgcc aggtcgcgtt ctggtcaacc tgacgagcgg tacgtcgcgt 300
gaagcccgtg agaacgcaga acatactgcg gctcagggag ccacctacct ggatggtgtg 360
attctgacca ttccaagcgg cattggcacc ccagaagcta ccattctgta ttcgggaccg 420
cgcagtacct tcgatgaaca tgaaagcgca ctgcgcgcat taggtccggg ggcgaccttt 480
gtaggggaag atcacggact ggctaacctg tatgatgcag caatgtcgag catgatgtgg 540
ggcctgctga atggtttcct gcatggaacc gcactgctgg gaaccgcggg cgtttcggca 600
acctctgtgg ttccgatgct gggtaaaggt tgggaagcga tgactggtat aattgcgcgc 660
tacgcagatc agattgatgc cggtgtctat ccagcagacg atgccacgat tgatacccag 720
cgtgctgcaa tgcagcacct ggtcgaggag agccgtgcgg cgggcatcaa tggcgaactt 780
ccggcgtttg tgaccaaact ggccgatcgc gcagtggcag aaggtcgtgg tggtgactct 840
tacgctgcac tgattgaaca gtttcgcaag tcagcttaa                        879

 
<210> 266
<211> 292
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Imine reductase variant from Pseudonocardia spinosispora
 
<400> 266
Met Ser Asp Ser Pro Val Pro Val Thr Val Leu Gly Leu Gly Leu Met 
1               5                   10                  15      
Gly Gln Ala Leu Ala Gly Ala Phe Leu Ala Ala Gly His Pro Thr Thr 
            20                  25                  30          
Val Trp Asn Arg Ser Ala Ala Lys Ala Asp Glu Leu Val Gly Arg Gly 
        35                  40                  45              
Ala Thr Leu Ala Ala Ser Pro Ala Ala Ala Val Ala Ala Gly Ser Leu 
    50                  55                  60                  
Ile Val Val Cys Val Thr Asp His Arg Ala Val Arg Glu Leu Leu Glu 
65                  70                  75                  80  
Pro Leu Gly Asp Ala Leu Pro Gly Arg Val Leu Val Asn Leu Thr Ser 
                85                  90                  95      
Gly Thr Ser Arg Glu Ala Arg Glu Asn Ala Glu His Thr Ala Ala Gln 
            100                 105                 110         
Gly Ala Thr Tyr Leu Asp Gly Val Ile Leu Thr Ile Pro Ser Gly Ile 
        115                 120                 125             
Gly Thr Pro Glu Ala Thr Ile Leu Tyr Ser Gly Pro Arg Ser Thr Phe 
    130                 135                 140                 
Asp Glu His Glu Ser Ala Leu Arg Ala Leu Gly Pro Gly Ala Thr Phe 
145                 150                 155                 160 
Val Gly Glu Asp His Gly Leu Ala Asn Leu Tyr Asp Ala Ala Met Ser 
                165                 170                 175     
Ser Met Met Trp Gly Leu Leu Asn Gly Phe Leu His Gly Thr Ala Leu 
            180                 185                 190         
Leu Gly Thr Ala Gly Val Ser Ala Thr Ser Val Val Pro Met Leu Gly 
        195                 200                 205             
Lys Gly Trp Glu Ala Met Thr Gly Ile Ile Ala Arg Tyr Ala Asp Gln 
    210                 215                 220                 
Ile Asp Ala Gly Val Tyr Pro Ala Asp Asp Ala Thr Ile Asp Thr Gln 
225                 230                 235                 240 
Arg Ala Ala Met Gln His Leu Val Glu Glu Ser Arg Ala Ala Gly Ile 
                245                 250                 255     
Asn Gly Glu Leu Pro Ala Phe Val Thr Lys Leu Ala Asp Arg Ala Val 
            260                 265                 270         
Ala Glu Gly Arg Gly Gly Asp Ser Tyr Ala Ala Leu Ile Glu Gln Phe 
        275                 280                 285             
Arg Lys Ser Ala                                                 
    290                                                         

 
<210> 267
<211> 879
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Imine reductase variant from Pseudonocardia spinosispora
 
<400> 267
atgagtgatt cacctgtgcc cgtgacagtt ctgggtcttg gtctgatggg tcaggcctta 60
gcgggagcgt ttctggcagc cggtcaccct acgacggttt ggaatcgcag cgcagctaaa 120
gctgatgaac tggttggtcg cggtgcaacc ctggccgcgt ctcccgcggc tgccgtcgca 180
gcgggtagtc tgatcgttgt ttgcgtcaca gatcatcgtg cggtgcgcga actgctggaa 240
ccacttggcg atgcactgcc aggtcgcgtt ctggtcaacc tgacgagcgg tacgtcgcgt 300
gaagcccgtg agaacgcaga acatactgcg gctcagggag ccacctacct ggatggtgtg 360
attctgacca ttccaagcgg cattggcacc ccagaagcta ggattctgta ttcgggaccg 420
cgcagtacct tcgatgaaca tgaaagcgca ctgcgcgcat taggtccggg ggcgaccttt 480
gtaggggaag atcacggact ggctaacctg tatgatgcag caatgtcgag catgatgtgg 540
ggcctgctga atggtttcct gcatggaacc gcactgctgg gaaccgcggg cgtttcggca 600
acctctgtgg ttccgatgct gggtcaaggt tgggaagcga tgactggtat aattgcgcgc 660
tacgcagatc agattgatgc cggtgtctat ccagcagacg atgccacgat tgatacccag 720
cgtgctgcaa tgcagcacct ggtcgaggag agccgtgcgg cgggcatcaa tggcgaactt 780
ccggcgtttg tgaccaaact ggccgatcgc gcagtggcag aaggtcgtgg tggtgactct 840
tacgctgcac tgattgaaca gtttcgcaag tcagcttaa                        879

 
<210> 268
<211> 292
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Imine reductase variant from Pseudonocardia spinosispora
 
<400> 268
Met Ser Asp Ser Pro Val Pro Val Thr Val Leu Gly Leu Gly Leu Met 
1               5                   10                  15      
Gly Gln Ala Leu Ala Gly Ala Phe Leu Ala Ala Gly His Pro Thr Thr 
            20                  25                  30          
Val Trp Asn Arg Ser Ala Ala Lys Ala Asp Glu Leu Val Gly Arg Gly 
        35                  40                  45              
Ala Thr Leu Ala Ala Ser Pro Ala Ala Ala Val Ala Ala Gly Ser Leu 
    50                  55                  60                  
Ile Val Val Cys Val Thr Asp His Arg Ala Val Arg Glu Leu Leu Glu 
65                  70                  75                  80  
Pro Leu Gly Asp Ala Leu Pro Gly Arg Val Leu Val Asn Leu Thr Ser 
                85                  90                  95      
Gly Thr Ser Arg Glu Ala Arg Glu Asn Ala Glu His Thr Ala Ala Gln 
            100                 105                 110         
Gly Ala Thr Tyr Leu Asp Gly Val Ile Leu Thr Ile Pro Ser Gly Ile 
        115                 120                 125             
Gly Thr Pro Glu Ala Arg Ile Leu Tyr Ser Gly Pro Arg Ser Thr Phe 
    130                 135                 140                 
Asp Glu His Glu Ser Ala Leu Arg Ala Leu Gly Pro Gly Ala Thr Phe 
145                 150                 155                 160 
Val Gly Glu Asp His Gly Leu Ala Asn Leu Tyr Asp Ala Ala Met Ser 
                165                 170                 175     
Ser Met Met Trp Gly Leu Leu Asn Gly Phe Leu His Gly Thr Ala Leu 
            180                 185                 190         
Leu Gly Thr Ala Gly Val Ser Ala Thr Ser Val Val Pro Met Leu Gly 
        195                 200                 205             
Gln Gly Trp Glu Ala Met Thr Gly Ile Ile Ala Arg Tyr Ala Asp Gln 
    210                 215                 220                 
Ile Asp Ala Gly Val Tyr Pro Ala Asp Asp Ala Thr Ile Asp Thr Gln 
225                 230                 235                 240 
Arg Ala Ala Met Gln His Leu Val Glu Glu Ser Arg Ala Ala Gly Ile 
                245                 250                 255     
Asn Gly Glu Leu Pro Ala Phe Val Thr Lys Leu Ala Asp Arg Ala Val 
            260                 265                 270         
Ala Glu Gly Arg Gly Gly Asp Ser Tyr Ala Ala Leu Ile Glu Gln Phe 
        275                 280                 285             
Arg Lys Ser Ala                                                 
    290                                                         


<210> 269
<211> 879
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Imine reductase variant from Pseudonocardia spinosispora
 
<400> 269
atgagtgatt cacctgtgcc cgtgacagtt ctgggtcttg gtctgatggg tcaggcctta 60
gcgggagcgt ttctggcagc cggtcaccct acgacggttt ggaatcgcag cgcagctaaa 120
gctgatgaac tggttggtcg cggtgcaacc ctggccgcgt ctcccgcggc tgccgtcgca 180
gcgggtagtc tgatcgttgt ttgcgtcaca gatcatcgtg cggtgcgcga actgctggaa 240
ccacttggcg atgcactgcc aggtcgcgtt ctggtcaacc tgacgagcgg tacgtcgcgt 300
gaagcccgtg agaacgcaga acatactgcg gctcagggag ccacctacct ggatggtgtg 360
attctgacca ttccaagcgg cattggcacc ccagaagcta ccattctgta ttcgggaccg 420
cgcagtacct tcgatgaaca tgaaagcgca ctgcgcgcat taggtccggg ggcgaccttt 480
gtaggggaag atcacggact ggctgcactg tatgatgcag caatgctgag cgtcatgtgg 540
ggcctgctga atggtttcct gcatggaacc gcactgctgg gaaccgcggg cgtttcggca 600
acctctgtgg ttccgatgct gggtcaaggt atcgaagcgg tgaccggttg gattgcgcgc 660
tacgcagatc agattgatgc cggtgtctat ccagcagacg acgccacgat tgatacccag 720
cgtgctgcaa tgcagcacct ggtcgaggag agccgtgcgg cgggcatcaa tggcgaactt 780
ccggcgtttg tgaccaaact ggccgatcgc gcagtggcag aaggtcgtgg tggtgactct 840
tacgctgcac tgattgaaca gtttcgcaag tcagcttaa                        879

 
<210> 270
<211> 292
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Imine reductase variant from Pseudonocardia spinosispora
 
<400> 270
Met Ser Asp Ser Pro Val Pro Val Thr Val Leu Gly Leu Gly Leu Met 
1               5                   10                  15      
Gly Gln Ala Leu Ala Gly Ala Phe Leu Ala Ala Gly His Pro Thr Thr 
            20                  25                  30          
Val Trp Asn Arg Ser Ala Ala Lys Ala Asp Glu Leu Val Gly Arg Gly 
        35                  40                  45              
Ala Thr Leu Ala Ala Ser Pro Ala Ala Ala Val Ala Ala Gly Ser Leu 
    50                  55                  60                  
Ile Val Val Cys Val Thr Asp His Arg Ala Val Arg Glu Leu Leu Glu 
65                  70                  75                  80  
Pro Leu Gly Asp Ala Leu Pro Gly Arg Val Leu Val Asn Leu Thr Ser 
                85                  90                  95      
Gly Thr Ser Arg Glu Ala Arg Glu Asn Ala Glu His Thr Ala Ala Gln 
            100                 105                 110         
Gly Ala Thr Tyr Leu Asp Gly Val Ile Leu Thr Ile Pro Ser Gly Ile 
        115                 120                 125             
Gly Thr Pro Glu Ala Thr Ile Leu Tyr Ser Gly Pro Arg Ser Thr Phe 
    130                 135                 140                 
Asp Glu His Glu Ser Ala Leu Arg Ala Leu Gly Pro Gly Ala Thr Phe 
145                 150                 155                 160 
Val Gly Glu Asp His Gly Leu Ala Ala Leu Tyr Asp Ala Ala Met Leu 
                165                 170                 175     
Ser Val Met Trp Gly Leu Leu Asn Gly Phe Leu His Gly Thr Ala Leu 
            180                 185                 190         
Leu Gly Thr Ala Gly Val Ser Ala Thr Ser Val Val Pro Met Leu Gly 
        195                 200                 205             
Gln Gly Ile Glu Ala Val Thr Gly Trp Ile Ala Arg Tyr Ala Asp Gln 
    210                 215                 220                 
Ile Asp Ala Gly Val Tyr Pro Ala Asp Asp Ala Thr Ile Asp Thr Gln 
225                 230                 235                 240 
Arg Ala Ala Met Gln His Leu Val Glu Glu Ser Arg Ala Ala Gly Ile 
                245                 250                 255     
Asn Gly Glu Leu Pro Ala Phe Val Thr Lys Leu Ala Asp Arg Ala Val 
            260                 265                 270         
Ala Glu Gly Arg Gly Gly Asp Ser Tyr Ala Ala Leu Ile Glu Gln Phe 
        275                 280                 285             
Arg Lys Ser Ala                                                 
    290             
	