                               SEQUENCE LISTING

<110> TWIST BIOSCIENCE CORPORATION
 
<120> DE NOVO SYNTHESIZED NUCLEIC ACID LIBRARIES

<130> 44854-727.601

<140>
<141>

<150> 62/419,881
<151> 2016-11-09

<150> 62/411,338
<151> 2016-10-21

<150> 62/393,948
<151> 2016-09-13

<150> 62/382,191
<151> 2016-08-31

<150> 62/378,134
<151> 2016-08-22

<160> 44    

<170> PatentIn version 3.5

<210> 1
<211> 62
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      oligonucleotide


<220>
<221> modified_base
<222> (51)..(52)
<223> Thymidine-succinyl hexamide CED phosphoramidite

<400> 1
agacaatcaa ccatttgggg tggacagcct tgacctctag acttcggcat tttttttttt       60

tt                                                                      62


<210> 2
<211> 112
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      polynucleotide


<220>
<221> modified_base
<222> (101)..(102)
<223> Thymidine-succinyl hexamide CED phosphoramidite

<400> 2
cgggatcctt atcgtcatcg tcgtacagat cccgacccat ttgctgtcca ccagtcatgc       60

tagccatacc atgatgatga tgatgatgag aaccccgcat tttttttttt tt              112


<210> 3
<211> 19
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<400> 3
atgcggggtt ctcatcatc                                                    19


<210> 4
<211> 20
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<400> 4
cgggatcctt atcgtcatcg                                                   20


<210> 5
<211> 42
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      oligonucleotide

<400> 5
ataactcaat ttgtaaaaaa gttttagagc tatgctgttt tg                          42


<210> 6
<211> 86
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      oligonucleotide

<400> 6
ggaaccattc aaaacagcat agcaagttaa aataaggcta gtccgttatc aacttgaaaa       60

agtggcaccg agtcggtgct tttttt                                            86


<210> 7
<211> 38
<212> DNA
<213> Streptococcus thermophilus

<400> 7
tactcaactt gaaaaggtgg caccgattcg gtgttttt                               38


<210> 8
<211> 39
<212> DNA
<213> Streptococcus mutans

<400> 8
tacacaactt gaaaaagtgc gcaccgattc ggtgctttt                              39


<210> 9
<211> 40
<212> DNA
<213> Listeria innocua

<400> 9
ttatcaactt ttaattaagt agcgctgttt cggcgctttt                             40


<210> 10
<211> 40
<212> DNA
<213> Mycoplasma mobile

<400> 10
tatgccgtaa ctactactta ttttcaaaat aagtagtttt                             40


<210> 11
<211> 36
<212> DNA
<213> Campylobacter jejuni

<400> 11
gactctgcgg ggttacaatc ccctaaaacc gctttt                                 36


<210> 12
<211> 117
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      polynucleotide

<400> 12
taatacgact cactataggg gatgcgcgca gttgtccgtt ttagagctag aaatagcaag       60

ttaaaataag gctagtccgt tatcaacttg aaaaagtggc accgagtcgg tgctttt         117


<210> 13
<211> 17
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      oligonucleotide

<400> 13
taatacgact cactata                                                      17


<210> 14
<211> 18
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      oligonucleotide

<400> 14
ggatgcgcgc agttgtcc                                                     18


<210> 15
<211> 80
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      oligonucleotide

<400> 15
gttttagagc tagaaatagc aagttaaaat aaggctagtc cgttatcaac ttgaaaaagt       60

ggcaccgagt cggtgctttt                                                   80


<210> 16
<211> 121
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      polynucleotide

<400> 16
gagctaatac gactcactat aggggatgcg cgcagttgtc cgttttagag ctagaaatag       60

caagttaaaa taaggctagt ccgttatcaa cttgaaaaag tggcaccgag tcggtgcttt      120

t                                                                      121


<210> 17
<211> 122
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      polynucleotide

<400> 17
cgagctaata cgactcacta taggggatgc gcgcagttgt ccgttttaga gctagaaata       60

gcaagttaaa ataaggctag tccgttatca acttgaaaaa gtggcaccga gtcggtgctt      120

tt                                                                     122


<210> 18
<211> 19
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      oligonucleotide

<400> 18
taatacgact cactatagg                                                    19


<210> 19
<211> 80
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      oligonucleotide

<400> 19
aaaagcaccg actcggtgcc actttttcaa gttgataacg gactagcctt attttaactt       60

gctatttcta gctctaaaac                                                   80


<210> 20
<211> 58
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      oligonucleotide

<400> 20
taatacgact cactataggg gatgcgcgca gttgtccgtt ttagagctag aaatagca         58


<210> 21
<211> 65
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      oligonucleotide

<400> 21
gaaattaata cgactcacta taggggatgc gcgcagttgt ccgttttaga gctagaaata       60

gcaag                                                                   65


<210> 22
<211> 66
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      oligonucleotide

<400> 22
gagctaatac gactcactat aggggatgcg cgcagttgtc cgttttagag ctagaaatag       60

caagtt                                                                  66


<210> 23
<211> 75
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      oligonucleotide

<400> 23
gagctaatac gactcactat aggggatgcg cgcagttgtc cgttttagag ctagaaatag       60

caagttaaaa taagg                                                        75


<210> 24
<211> 14
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      oligonucleotide

<400> 24
aaaagcaccg actc                                                         14


<210> 25
<211> 15
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      oligonucleotide

<400> 25
aaaagcaccg actcg                                                        15


<210> 26
<211> 16
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      oligonucleotide

<400> 26
aaaagcaccg actcgg                                                       16


<210> 27
<211> 17
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      oligonucleotide

<400> 27
aaaagcaccg actcggt                                                      17


<210> 28
<211> 24
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      oligonucleotide

<400> 28
gaaattaata cgactcacta tagg                                              24


<210> 29
<211> 23
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      oligonucleotide

<400> 29
gagctaatac gactcactat agg                                               23


<210> 30
<211> 25
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      oligonucleotide

<400> 30
gcgagctaat acgactcact atagg                                             25


<210> 31
<211> 120
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      polynucleotide

<400> 31
cgagctaata cgactcacta taggggcaca acgtggagga tggcagcgtg cagctggctg       60

atcactacca gcaaaacact ccaatcggtg atggtcctgt tgcaccgagt cggtgctttt      120


<210> 32
<211> 71
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      oligonucleotide

<400> 32
cgagctaata cgactcacta taggggcaca acgtggagga tggcagcgtg cagctggctg       60

atcactacca g                                                            71


<210> 33
<211> 69
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      oligonucleotide

<400> 33
aaagcaccga ctcggtgcaa caggaccatc accgattgga gtgttttgct ggtagtgatc       60

agccagctg                                                               69


<210> 34
<211> 130
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      polynucleotide


<220>
<221> modified_base
<222> (25)..(42)
<223> a, c, t, g, unknown or other

<400> 34
cgagctaata cgactcacta taggnnnnnn nnnnnnnnnn nngttttaga gctatgctga       60

aaagcatagc aagttaaaat aaggctagtc cgttatcaac ttgaaaaagt ggcaccgagt      120

cggtgctttt                                                             130


<210> 35
<211> 720
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      polynucleotide

<400> 35
atgcgtaaag gcgaagagct gttcactggt gtcgtcccta ttctggtgga actggatggt       60

gatgtcaacg gtcataagtt ttccgtgcgt ggcgagggtg aaggtgacgc aactaatggt      120

aaactgacgc tgaagttcat ctgtactact ggtaaactgc cggtaccttg gccgactctg      180

gtaacgacgc tgacttatgg tgttcagtgc tttgctcgtt atccggacca tatgaagcag      240

catgacttct tcaagtccgc catgccggaa ggctatgtgc aggaacgcac gatttccttt      300

aaggatgacg gcacgtacaa aacgcgtgcg gaagtgaaat ttgaaggcga taccctggta      360

aaccgcattg agctgaaagg cattgacttt aaagaagacg gcaatatcct gggccataag      420

ctggaataca attttaacag ccacaatgtt tacatcaccg ccgataaaca aaaaaatggc      480

attaaagcga attttaaaat tcgccacaac gtggaggatg gcagcgtgca gctggctgat      540

cactaccagc aaaacactcc aatcggtgat ggtcctgttc tgctgccaga caatcactat      600

ctgagcacgc aaagcgttct gtctaaagat ccgaacgaga aacgcgatca tatggttctg      660

ctggagttcg taaccgcagc gggcatcacg catggtatgg atgaactgta caaatgataa      720


<210> 36
<211> 70
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      oligonucleotide

<400> 36
cgagctaata cgactcacta taggaacgca cgatttcctt tagttttaga gctatgctga       60

aaagcatagc                                                              70


<210> 37
<211> 70
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      oligonucleotide

<400> 37
cgagctaata cgactcacta taggcattga ctttaaagaa gagttttaga gctatgctga       60

aaagcatagc                                                              70


<210> 38
<211> 70
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      oligonucleotide

<400> 38
cgagctaata cgactcacta taggaggatg gcagcgtgca gcgttttaga gctatgctga       60

aaagcatagc                                                              70


<210> 39
<211> 88
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      oligonucleotide

<400> 39
aaaagcaccg actcggtgcc actttttcaa gttgataacg gactagcctt attttaactt       60

gctatgcttt tcagcatagc tctaaaac                                          88


<210> 40
<211> 103
<212> RNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      polynucleotide


<220>
<221> modified_base
<222> (1)..(20)
<223> a, c, u, g, unknown or other

<400> 40
nnnnnnnnnn nnnnnnnnnn guuuuagagc uagaaauagc aaguuaaaau aaggcuaguc       60

cguuaucaac uugaaaaagu ggcaccgagu cggugcuuuu uuu                        103


<210> 41
<211> 76
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      oligonucleotide

<400> 41
cgagctaata cgactcacta taggggatgc gcgcagttgt ccgttttaga gctagaaata       60

gcaagttaaa ataagg                                                       76


<210> 42
<211> 30
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      oligonucleotide

<400> 42
taaggctagt ccgttatcaa cttgaaaaag                                        30


<210> 43
<211> 27
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      oligonucleotide

<400> 43
ctagtccgtt atcaacttga aaaagtg                                           27


<210> 44
<211> 122
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      polynucleotide

<400> 44
gaaattaata cgactcacta taggggatgc gcgcagttgt ccgttttaga gctagaaata       60

gcaagttaaa ataaggctag tccgttatca acttgaaaaa gtggcaccga gtcggtgctt      120

tt                                                                     122

