                         SEQUENCE LISTING

<110>  Whitehead Institute for Biomedical Research
 
<120>  METHODS OF EDITING DNA METHYLATION

<130>  WIBR-158-WO1

<140>  PCT/US2017/047674
<141>  2017-08-18

<150>  62/377,520
<151>  2016-08-19

<160>  167   

<170>  PatentIn version 3.5

<210>  1
<211>  23
<212>  DNA
<213>  Artificial

<220>
<223>  guide RNA sequence

<400>  1
gttttgaggc ctcatttgta agg                                               23


<210>  2
<211>  23
<212>  DNA
<213>  Artificial

<220>
<223>  guide RNA sequence

<400>  2
tttttgaaaa attaccttgt ggg                                               23


<210>  3
<211>  23
<212>  DNA
<213>  Artificial

<220>
<223>  guide RNA sequence

<400>  3
cagagtccta gacatttcca tgg                                               23


<210>  4
<211>  23
<212>  DNA
<213>  Artificial

<220>
<223>  guide RNA sequence

<400>  4
ggagagaggc ctcatttgta ggg                                               23


<210>  5
<211>  23
<212>  DNA
<213>  Artificial

<220>
<223>  guide RNA sequence

<400>  5
gttgtcagag catagcttgt agg                                               23


<210>  6
<211>  23
<212>  DNA
<213>  Artificial

<220>
<223>  guide RNA sequence

<400>  6
ctaaagcgat gttccttcca ggg                                               23


<210>  7
<211>  23
<212>  DNA
<213>  Artificial

<220>
<223>  guide RNA sequence

<400>  7
ttgctctcaa gacatttcca ggg                                               23


<210>  8
<211>  23
<212>  DNA
<213>  Artificial

<220>
<223>  guide RNA sequence

<400>  8
ttgctttcca ttttcttcca ggg                                               23


<210>  9
<211>  23
<212>  DNA
<213>  Artificial

<220>
<223>  guide RNA sequence

<400>  9
agcttctagc tctaccttct agg                                               23


<210>  10
<211>  23
<212>  DNA
<213>  Artificial

<220>
<223>  guide RNA sequence

<400>  10
tctgcgagtc cttaatttta ggg                                               23


<210>  11
<211>  23
<212>  DNA
<213>  Artificial

<220>
<223>  guide RNA sequence

<400>  11
aaagcatttt cttctttcca ggg                                               23


<210>  12
<211>  23
<212>  DNA
<213>  Artificial

<220>
<223>  guide RNA sequence

<400>  12
agttgctgct cttttgtcca ggg                                               23


<210>  13
<211>  23
<212>  DNA
<213>  Artificial

<220>
<223>  guide RNA sequence

<400>  13
acgcaactgc gagctttcaa agg                                               23


<210>  14
<211>  23
<212>  DNA
<213>  Artificial

<220>
<223>  guide RNA sequence

<400>  14
tttaaagccc cttccttcca ggg                                               23


<210>  15
<211>  23
<212>  DNA
<213>  Artificial

<220>
<223>  guide RNA sequence

<400>  15
actgtcttca acttccttgg ggg                                               23


<210>  16
<211>  23
<212>  DNA
<213>  Artificial

<220>
<223>  guide RNA sequence

<400>  16
ggactactga gacatttcca ggg                                               23


<210>  17
<211>  23
<212>  DNA
<213>  Artificial

<220>
<223>  guide RNA sequence

<400>  17
tcaaccctga gacatttcca ggg                                               23


<210>  18
<211>  23
<212>  DNA
<213>  Artificial

<220>
<223>  guide RNA sequence

<400>  18
ccaagattga gacatttcca ggg                                               23


<210>  19
<211>  23
<212>  DNA
<213>  Artificial

<220>
<223>  guide RNA sequence

<400>  19
taaagtccca gtcccctccc tgg                                               23


<210>  20
<211>  23
<212>  DNA
<213>  Artificial

<220>
<223>  guide RNA sequence

<400>  20
ctaagctctg tgttgttcca ggg                                               23


<210>  21
<211>  23
<212>  DNA
<213>  Artificial

<220>
<223>  guide RNA sequence

<400>  21
gtagagcatg ttgagctgta ggg                                               23


<210>  22
<211>  23
<212>  DNA
<213>  Artificial

<220>
<223>  guide RNA sequence

<400>  22
gagccgagct gtagggtgct tgg                                               23


<210>  23
<211>  23
<212>  DNA
<213>  Artificial

<220>
<223>  guide RNA sequence

<400>  23
cgggcagcct gtagggtgct ggg                                               23


<210>  24
<211>  23
<212>  DNA
<213>  Artificial

<220>
<223>  guide RNA sequence

<400>  24
gcgcaagggt tgtcgtgcct tgg                                               23


<210>  25
<211>  23
<212>  DNA
<213>  Artificial

<220>
<223>  guide RNA sequence

<400>  25
cgcatgtgca gccattgcct ggg                                               23


<210>  26
<211>  23
<212>  DNA
<213>  Artificial

<220>
<223>  guide RNA sequence

<400>  26
tttggtagct gccttttggc agg                                               23


<210>  27
<211>  20
<212>  DNA
<213>  Artificial

<220>
<223>  guide RNA sequence

<400>  27
acaaacctga gccattgcgg                                                   20


<210>  28
<211>  23
<212>  DNA
<213>  Artificial

<220>
<223>  guide RNA sequence

<400>  28
ccctgcagct gtagggtgct agg                                               23


<210>  29
<211>  23
<212>  DNA
<213>  Artificial

<220>
<223>  guide RNA sequence

<400>  29
tctcttcagt gtaattggct tgg                                               23


<210>  30
<211>  23
<212>  DNA
<213>  Artificial

<220>
<223>  guide RNA sequence

<400>  30
tccatttgct gtagggtgct agg                                               23


<210>  31
<211>  23
<212>  DNA
<213>  Artificial

<220>
<223>  guide RNA sequence

<400>  31
tgctaatgtc cccatttggc agg                                               23


<210>  32
<211>  23
<212>  DNA
<213>  Artificial

<220>
<223>  guide RNA sequence

<400>  32
gcacagcact gtagggtgct ggg                                               23


<210>  33
<211>  23
<212>  DNA
<213>  Artificial

<220>
<223>  guide RNA sequence

<400>  33
ccatctctct gtagggtgct ggg                                               23


<210>  34
<211>  23
<212>  DNA
<213>  Artificial

<220>
<223>  guide RNA sequence

<400>  34
gtgagctgct gtagggtgct agg                                               23


<210>  35
<211>  23
<212>  DNA
<213>  Artificial

<220>
<223>  guide RNA sequence

<400>  35
gaatggacct gtagggtgct tgg                                               23


<210>  36
<211>  24
<212>  DNA
<213>  Artificial

<220>
<223>  guide RNA primer sequence

<400>  36
ttggcccccg ggggaaaaat tttt                                              24


<210>  37
<211>  24
<212>  DNA
<213>  Artificial

<220>
<223>  guide RNA primer sequence

<400>  37
aaacaaaaat ttttcccccg gggg                                              24


<210>  38
<211>  24
<212>  DNA
<213>  Artificial

<220>
<223>  guide RNA primer sequence

<400>  38
ttggctacaa agcatgcaat gccc                                              24


<210>  39
<211>  24
<212>  DNA
<213>  Artificial

<220>
<223>  guide RNA primer sequence

<400>  39
aaacgggcat tgcatgcttt gtag                                              24


<210>  40
<211>  24
<212>  DNA
<213>  Artificial

<220>
<223>  guide RNA primer sequence

<400>  40
ttggaatgcg cggaattctg attc                                              24


<210>  41
<211>  24
<212>  DNA
<213>  Artificial

<220>
<223>  guide RNA primer sequence

<400>  41
aaacgaatca gaattccgcg catt                                              24


<210>  42
<211>  24
<212>  DNA
<213>  Artificial

<220>
<223>  guide RNA primer sequence

<400>  42
ttgggtgggt gggagtccac gaga                                              24


<210>  43
<211>  24
<212>  DNA
<213>  Artificial

<220>
<223>  guide RNA primer sequence

<400>  43
aaactctcgt ggactcccac ccac                                              24


<210>  44
<211>  24
<212>  DNA
<213>  Artificial

<220>
<223>  guide RNA primer sequence

<400>  44
ttggggcagc gtggagccct ctcg                                              24


<210>  45
<211>  24
<212>  DNA
<213>  Artificial

<220>
<223>  guide RNA primer sequence

<400>  45
aaaccgagag ggctccacgc tgcc                                              24


<210>  46
<211>  24
<212>  DNA
<213>  Artificial

<220>
<223>  guide RNA primer sequence

<400>  46
ttgggagccg agctgtaggg tgct                                              24


<210>  47
<211>  24
<212>  DNA
<213>  Artificial

<220>
<223>  guide RNA primer sequence

<400>  47
aaacagcacc ctacagctcg gctc                                              24


<210>  48
<211>  24
<212>  DNA
<213>  Artificial

<220>
<223>  guide RNA primer sequence

<400>  48
ttggtttggt agctgccttt tggc                                              24


<210>  49
<211>  24
<212>  DNA
<213>  Artificial

<220>
<223>  guide RNA primer sequence

<400>  49
aaacgccaaa aggcagctac caaa                                              24


<210>  50
<211>  24
<212>  DNA
<213>  Artificial

<220>
<223>  guide RNA primer sequence

<400>  50
ttggcgcatg tgcagccatt gcct                                              24


<210>  51
<211>  24
<212>  DNA
<213>  Artificial

<220>
<223>  guide RNA primer sequence

<400>  51
aaacaggcaa tggctgcaca tgcg                                              24


<210>  52
<211>  21
<212>  DNA
<213>  Artificial

<220>
<223>  guide RNA primer sequence

<400>  52
ttggacaaac ctgagccatt g                                                 21


<210>  53
<211>  21
<212>  DNA
<213>  Artificial

<220>
<223>  guide RNA primer sequence

<400>  53
aaaccaatgg ctcaggtttg t                                                 21


<210>  54
<211>  24
<212>  DNA
<213>  Artificial

<220>
<223>  guide RNA primer sequence

<400>  54
ttggagcatt tgggggcatt tatg                                              24


<210>  55
<211>  24
<212>  DNA
<213>  Artificial

<220>
<223>  guide RNA primer sequence

<400>  55
aaaccataaa tgcccccaaa tgct                                              24


<210>  56
<211>  24
<212>  DNA
<213>  Artificial

<220>
<223>  guide RNA primer sequence

<400>  56
ttggaagtat cctcctccag cagc                                              24


<210>  57
<211>  24
<212>  DNA
<213>  Artificial

<220>
<223>  guide RNA primer sequence

<400>  57
aaacgctgct ggaggaggat actt                                              24


<210>  58
<211>  24
<212>  DNA
<213>  Artificial

<220>
<223>  guide RNA primer sequence

<400>  58
ttggacacag ccagttgggg gaag                                              24


<210>  59
<211>  24
<212>  DNA
<213>  Artificial

<220>
<223>  guide RNA primer sequence

<400>  59
aaaccttccc ccaactggct gtgt                                              24


<210>  60
<211>  22
<212>  DNA
<213>  Artificial

<220>
<223>  guide RNA primer sequence

<400>  60
ttggccagag tcagctgttc ct                                                22


<210>  61
<211>  22
<212>  DNA
<213>  Artificial

<220>
<223>  guide RNA primer sequence

<400>  61
aaacaggaac agctgactct gg                                                22


<210>  62
<211>  24
<212>  DNA
<213>  Artificial

<220>
<223>  guide RNA primer sequence

<400>  62
ttgggttttg aggcctcatt tgta                                              24


<210>  63
<211>  24
<212>  DNA
<213>  Artificial

<220>
<223>  guide RNA primer sequence

<400>  63
aaactacaaa tgaggcctca aaac                                              24


<210>  64
<211>  24
<212>  DNA
<213>  Artificial

<220>
<223>  guide RNA primer sequence

<400>  64
ttggtttttg aaaaattacc ttgt                                              24


<210>  65
<211>  24
<212>  DNA
<213>  Artificial

<220>
<223>  guide RNA primer sequence

<400>  65
aaacacaagg taatttttca aaaa                                              24


<210>  66
<211>  24
<212>  DNA
<213>  Artificial

<220>
<223>  guide RNA primer sequence

<400>  66
ttggcagagt cctagacatt tcca                                              24


<210>  67
<211>  24
<212>  DNA
<213>  Artificial

<220>
<223>  guide RNA primer sequence

<400>  67
aaactggaaa tgtctaggac tctg                                              24


<210>  68
<211>  24
<212>  DNA
<213>  Artificial

<220>
<223>  guide RNA primer sequence

<400>  68
ttggtctcac ccttgatagt ttga                                              24


<210>  69
<211>  24
<212>  DNA
<213>  Artificial

<220>
<223>  guide RNA primer sequence

<400>  69
aaactcaaac tatcaagggt gaga                                              24


<210>  70
<211>  24
<212>  DNA
<213>  Artificial

<220>
<223>  guide RNA primer sequence

<400>  70
ttggggtaaa tctttgaagc caat                                              24


<210>  71
<211>  24
<212>  DNA
<213>  Artificial

<220>
<223>  guide RNA primer sequence

<400>  71
aaacattggc ttcaaagatt tacc                                              24


<210>  72
<211>  24
<212>  DNA
<213>  Artificial

<220>
<223>  guide RNA primer sequence

<400>  72
ttggattttc tacctacggt gtgc                                              24


<210>  73
<211>  24
<212>  DNA
<213>  Artificial

<220>
<223>  guide RNA primer sequence

<400>  73
aaacgcacac cgtaggtaga aaat                                              24


<210>  74
<211>  24
<212>  DNA
<213>  Artificial

<220>
<223>  guide RNA primer sequence

<400>  74
ttggtctcct ggaaggactc tggg                                              24


<210>  75
<211>  24
<212>  DNA
<213>  Artificial

<220>
<223>  guide RNA primer sequence

<400>  75
aaaccccaga gtccttccag gaga                                              24


<210>  76
<211>  24
<212>  DNA
<213>  Artificial

<220>
<223>  guide RNA primer sequence

<400>  76
ttggtcctcc ttccttgcca acgc                                              24


<210>  77
<211>  24
<212>  DNA
<213>  Artificial

<220>
<223>  guide RNA primer sequence

<400>  77
aaacgcgttg gcaaggaagg agga                                              24


<210>  78
<211>  24
<212>  DNA
<213>  Artificial

<220>
<223>  guide RNA primer sequence

<400>  78
ttggcccgct atttgctgtg ggtt                                              24


<210>  79
<211>  24
<212>  DNA
<213>  Artificial

<220>
<223>  guide RNA primer sequence

<400>  79
aaacaaccca cagcaaatag cggg                                              24


<210>  80
<211>  22
<212>  DNA
<213>  Artificial

<220>
<223>  qPCR primer sequence

<400>  80
caggagtaca tatcggccac ca                                                22


<210>  81
<211>  23
<212>  DNA
<213>  Artificial

<220>
<223>  qPCR primer sequence

<400>  81
gtaggccaag ttgccttgtc cgt                                               23


<210>  82
<211>  19
<212>  DNA
<213>  Artificial

<220>
<223>  qPCR primer sequence

<400>  82
gccttccttc ttgggtatg                                                    19


<210>  83
<211>  19
<212>  DNA
<213>  Artificial

<220>
<223>  qPCR primer sequence

<400>  83
accaccagac aacactgtg                                                    19


<210>  84
<211>  25
<212>  DNA
<213>  Artificial

<220>
<223>  qPCR primer sequence

<400>  84
gctatactca gaaggtccag aaggc                                             25


<210>  85
<211>  23
<212>  DNA
<213>  Artificial

<220>
<223>  qPCR primer sequence

<400>  85
tcagagaatg agggtagcac agc                                               23


<210>  86
<211>  22
<212>  DNA
<213>  Artificial

<220>
<223>  qPCR primer sequence

<400>  86
actttctgga gccctcctgg ca                                                22


<210>  87
<211>  21
<212>  DNA
<213>  Artificial

<220>
<223>  qPCR primer sequence

<400>  87
tttgttgcac tacacagcat g                                                 21


<210>  88
<211>  20
<212>  DNA
<213>  Artificial

<220>
<223>  qPCR primer sequence

<400>  88
aggggattct ccgtggtaca                                                   20


<210>  89
<211>  20
<212>  DNA
<213>  Artificial

<220>
<223>  qPCR primer sequence

<400>  89
ctcttcccct gtactcgcaa                                                   20


<210>  90
<211>  20
<212>  DNA
<213>  Artificial

<220>
<223>  qPCR primer sequence

<400>  90
cgagacgcgg atggatgtaa                                                   20


<210>  91
<211>  20
<212>  DNA
<213>  Artificial

<220>
<223>  qPCR primer sequence

<400>  91
gcggcacttt tcttccgatg                                                   20


<210>  92
<211>  21
<212>  DNA
<213>  Artificial

<220>
<223>  qPCR primer sequence

<400>  92
ccagaccctg catgagcttt a                                                 21


<210>  93
<211>  20
<212>  DNA
<213>  Artificial

<220>
<223>  qPCR primer sequence

<400>  93
aaacagccac aggactcgaa                                                   20


<210>  94
<211>  22
<212>  DNA
<213>  Artificial

<220>
<223>  qPCR primer sequence

<400>  94
tctgttaagg gagcagccat gc                                                22


<210>  95
<211>  21
<212>  DNA
<213>  Artificial

<220>
<223>  qPCR primer sequence

<400>  95
ggatattagc tgcaggaggc g                                                 21


<210>  96
<211>  20
<212>  DNA
<213>  Artificial

<220>
<223>  qPCR primer sequence

<400>  96
cagagagcca agggcaatga                                                   20


<210>  97
<211>  20
<212>  DNA
<213>  Artificial

<220>
<223>  qPCR primer sequence

<400>  97
ggaccccact ttacagccat                                                   20


<210>  98
<211>  21
<212>  DNA
<213>  Artificial

<220>
<223>  qPCR primer sequence

<400>  98
gcgttctctt tggaaaggtg t                                                 21


<210>  99
<211>  20
<212>  DNA
<213>  Artificial

<220>
<223>  qPCR primer sequence

<400>  99
ttgttgtcgg cttcctccac                                                   20


<210>  100
<211>  20
<212>  DNA
<213>  Artificial

<220>
<223>  qPCR primer sequence

<400>  100
atctctggag tccatgcgga                                                   20


<210>  101
<211>  20
<212>  DNA
<213>  Artificial

<220>
<223>  qPCR primer sequence

<400>  101
caaagtccct tggctgctgt                                                   20


<210>  102
<211>  20
<212>  DNA
<213>  Artificial

<220>
<223>  qPCR primer sequence

<400>  102
caggtgtgca aatcttgggt                                                   20


<210>  103
<211>  20
<212>  DNA
<213>  Artificial

<220>
<223>  qPCR primer sequence

<400>  103
tggtggcttg caatcatctg                                                   20


<210>  104
<211>  20
<212>  DNA
<213>  Artificial

<220>
<223>  qPCR primer sequence

<400>  104
tgagtccttg ctcggttctt                                                   20


<210>  105
<211>  20
<212>  DNA
<213>  Artificial

<220>
<223>  qPCR primer sequence

<400>  105
caggcacatt gctgtgagtt                                                   20


<210>  106
<211>  20
<212>  DNA
<213>  Artificial

<220>
<223>  qPCR primer sequence

<400>  106
tggccttgta ctgttgcaac                                                   20


<210>  107
<211>  20
<212>  DNA
<213>  Artificial

<220>
<223>  qPCR primer sequence

<400>  107
agtgtcacta tggccacctt                                                   20


<210>  108
<211>  20
<212>  DNA
<213>  Artificial

<220>
<223>  qPCR primer sequence

<400>  108
aatcttccct tgggggtatg                                                   20


<210>  109
<211>  20
<212>  DNA
<213>  Artificial

<220>
<223>  qPCR primer sequence

<400>  109
taaggagcca tgctgtaccc                                                   20


<210>  110
<211>  30
<212>  DNA
<213>  Artificial

<220>
<223>  bisulfite sequencing primer sequence

<400>  110
gaagtttttg tgaaataagt tttgggtagg                                        30


<210>  111
<211>  24
<212>  DNA
<213>  Artificial

<220>
<223>  bisulfite sequencing primer sequence

<400>  111
ctcgaccaaa ataaacacca cccc                                              24


<210>  112
<211>  25
<212>  DNA
<213>  Artificial

<220>
<223>  bisulfite sequencing primer sequence

<400>  112
cgagttgtag ggtgtttggt aattg                                             25


<210>  113
<211>  25
<212>  DNA
<213>  Artificial

<220>
<223>  bisulfite sequencing primer sequence

<400>  113
acgttacaaa tcactcctca aaacc                                             25


<210>  114
<211>  27
<212>  DNA
<213>  Artificial

<220>
<223>  bisulfite sequencing primer sequence

<400>  114
ggttgtagga gaagaaaatg agattag                                           27


<210>  115
<211>  24
<212>  DNA
<213>  Artificial

<220>
<223>  bisulfite sequencing primer sequence

<400>  115
ctcgaccaaa ataaacacca cccc                                              24


<210>  116
<211>  25
<212>  DNA
<213>  Artificial

<220>
<223>  bisulfite sequencing primer sequence

<400>  116
tagtttaagg gcgtagaggt ttgag                                             25


<210>  117
<211>  25
<212>  DNA
<213>  Artificial

<220>
<223>  bisulfite sequencing primer sequence

<400>  117
acgttacaaa tcactcctca aaacc                                             25


<210>  118
<211>  26
<212>  DNA
<213>  Artificial

<220>
<223>  bisulfite sequencing primer sequence

<400>  118
ttatttattg gttggattag aggggt                                            26


<210>  119
<211>  24
<212>  DNA
<213>  Artificial

<220>
<223>  bisulfite sequencing primer sequence

<400>  119
catatacttc ccaacaaacc aaac                                              24


<210>  120
<211>  26
<212>  DNA
<213>  Artificial

<220>
<223>  bisulfite sequencing primer sequence

<400>  120
gtgaatttgt taggattgga agtgaa                                            26


<210>  121
<211>  30
<212>  DNA
<213>  Artificial

<220>
<223>  bisulfite sequencing primer sequence

<400>  121
actcttacta tatatttccc cttctcttca                                        30


<210>  122
<211>  24
<212>  DNA
<213>  Artificial

<220>
<223>  bisulfite sequencing primer sequence

<400>  122
ggtttgaggt aggtaggggt tagg                                              24


<210>  123
<211>  30
<212>  DNA
<213>  Artificial

<220>
<223>  bisulfite sequencing primer sequence

<400>  123
ccaactcact ttctcccaaa attacactaa                                        30


<210>  124
<211>  29
<212>  DNA
<213>  Artificial

<220>
<223>  bisulfite sequencing primer sequence

<400>  124
gtagaatttg ttaggtgggt gaaaggaag                                         29


<210>  125
<211>  30
<212>  DNA
<213>  Artificial

<220>
<223>  bisulfite sequencing primer sequence

<400>  125
ccttcctccc aaaatactaa cctctcatac                                        30


<210>  126
<211>  28
<212>  DNA
<213>  Artificial

<220>
<223>  bisulfite sequencing primer sequence

<400>  126
gatttttggg tattgtattg gaagtggg                                          28


<210>  127
<211>  32
<212>  DNA
<213>  Artificial

<220>
<223>  bisulfite sequencing primer sequence

<400>  127
ccaaaatatt tattccctct actttaaaac ac                                     32


<210>  128
<211>  27
<212>  DNA
<213>  Artificial

<220>
<223>  bisulfite sequencing primer sequence

<400>  128
tttaggatag gatgggagta ttggttg                                           27


<210>  129
<211>  34
<212>  DNA
<213>  Artificial

<220>
<223>  bisulfite sequencing primer sequence

<400>  129
caaaatcact caaaatcatc ctattacata aaac                                   34


<210>  130
<211>  38
<212>  DNA
<213>  Artificial

<220>
<223>  bisulfite sequencing primer sequence

<400>  130
attaagaggt taggggtttt ttagttggtt ttgtattg                               38


<210>  131
<211>  37
<212>  DNA
<213>  Artificial

<220>
<223>  bisulfite sequencing primer sequence

<400>  131
aaaaaaaacc ttcatcacat aataaactaa accaacc                                37


<210>  132
<211>  28
<212>  DNA
<213>  Artificial

<220>
<223>  bisulfite sequencing primer sequence

<400>  132
gaaaggatgt aattagaggg tttttggg                                          28


<210>  133
<211>  25
<212>  DNA
<213>  Artificial

<220>
<223>  bisulfite sequencing primer sequence

<400>  133
aatcctttct caaaacccct tcctc                                             25


<210>  134
<211>  29
<212>  DNA
<213>  Artificial

<220>
<223>  bisulfite sequencing primer sequence

<400>  134
gtggggtttt tataattagg aaagaatag                                         29


<210>  135
<211>  25
<212>  DNA
<213>  Artificial

<220>
<223>  bisulfite sequencing primer sequence

<400>  135
attacaaacc ccttctaaaa actcc                                             25


<210>  136
<211>  23
<212>  DNA
<213>  Artificial

<220>
<223>  bisulfite sequencing primer sequence

<400>  136
atttggtagt taggaaggtt gta                                               23


<210>  137
<211>  22
<212>  DNA
<213>  Artificial

<220>
<223>  bisulfite sequencing primer sequence

<400>  137
ccaaaaaacc tccccttttt cc                                                22


<210>  138
<211>  25
<212>  DNA
<213>  Artificial

<220>
<223>  bisulfite sequencing primer sequence

<400>  138
gtggattttt ttaaggagtg tgttg                                             25


<210>  139
<211>  35
<212>  DNA
<213>  Artificial

<220>
<223>  bisulfite sequencing primer sequence

<400>  139
cttctaatat ctaaactcaa caacaatata tccac                                  35


<210>  140
<211>  27
<212>  DNA
<213>  Artificial

<220>
<223>  bisulfite sequencing primer sequence

<400>  140
ggagatttag gaagtatggg gttagtg                                           27


<210>  141
<211>  28
<212>  DNA
<213>  Artificial

<220>
<223>  bisulfite sequencing primer sequence

<400>  141
aaaacctcct ctcttacttt tctacttc                                          28


<210>  142
<211>  27
<212>  DNA
<213>  Artificial

<220>
<223>  3C assay primer sequence

<400>  142
cacatcttca aagcaaacac tattgtt                                           27


<210>  143
<211>  18
<212>  DNA
<213>  Artificial

<220>
<223>  3C assay primer sequence

<400>  143
ttcctggaac ctgggcaa                                                     18


<210>  144
<211>  23
<212>  DNA
<213>  Artificial

<220>
<223>  3C assay primer sequence

<400>  144
tgatacagca cagctttcct tca                                               23


<210>  145
<211>  28
<212>  DNA
<213>  Artificial

<220>
<223>  3C assay primer sequence

<400>  145
cagatttttt atttccttca gttctgtg                                          28


<210>  146
<211>  30
<212>  DNA
<213>  Artificial

<220>
<223>  3C assay primer sequence

<400>  146
tctcctaccc attgcttctc tgctacctgc                                        30


<210>  147
<211>  26
<212>  DNA
<213>  Artificial

<220>
<223>  3C assay primer sequence

<400>  147
tgaagtttga ggagatgcca tggttg                                            26


<210>  148
<211>  27
<212>  DNA
<213>  Artificial

<220>
<223>  3C assay primer sequence

<400>  148
cacgctaggc tgaacactgt gtcactg                                           27


<210>  149
<211>  20
<212>  DNA
<213>  Artificial

<220>
<223>  3C assay primer sequence

<400>  149
aggatggctc agcggttaag                                                   20


<210>  150
<211>  22
<212>  DNA
<213>  Artificial

<220>
<223>  3C assay primer sequence

<400>  150
agggctcacc ttcagtcaag tt                                                22


<210>  151
<211>  29
<212>  DNA
<213>  Artificial

<220>
<223>  3C assay primer sequence

<400>  151
cggcctgtct actttagcct cagactcca                                         29


<210>  152
<211>  28
<212>  DNA
<213>  Artificial

<220>
<223>  3C assay primer sequence

<400>  152
tgccttccct cttacaagga gttttctt                                          28


<210>  153
<211>  27
<212>  DNA
<213>  Artificial

<220>
<223>  3C assay primer sequence

<400>  153
cggttaagaa gagctcttct ggaggcc                                           27


<210>  154
<211>  23
<212>  DNA
<213>  Artificial

<220>
<223>  3C assay primer sequence

<400>  154
gggagtgact ctctgtccat tca                                               23


<210>  155
<211>  22
<212>  DNA
<213>  Artificial

<220>
<223>  3C assay primer sequence

<400>  155
atttgtgtgg cctcttgttt ga                                                22


<210>  156
<211>  18
<212>  DNA
<213>  Artificial

<220>
<223>  3C assay primer sequence

<400>  156
tccaggcccc gcgtgtcc                                                     18


<210>  157
<211>  2309
<212>  PRT
<213>  Artificial

<220>
<223>  dCAS9-Dnmt3a (protein)

<400>  157

Met Asp Lys Lys Tyr Ser Ile Gly Leu Ala Ile Gly Thr Asn Ser Val 
1               5                   10                  15      


Gly Trp Ala Val Ile Thr Asp Glu Tyr Lys Val Pro Ser Lys Lys Phe 
            20                  25                  30          


Lys Val Leu Gly Asn Thr Asp Arg His Ser Ile Lys Lys Asn Leu Ile 
        35                  40                  45              


Gly Ala Leu Leu Phe Asp Ser Gly Glu Thr Ala Glu Ala Thr Arg Leu 
    50                  55                  60                  


Lys Arg Thr Ala Arg Arg Arg Tyr Thr Arg Arg Lys Asn Arg Ile Cys 
65                  70                  75                  80  


Tyr Leu Gln Glu Ile Phe Ser Asn Glu Met Ala Lys Val Asp Asp Ser 
                85                  90                  95      


Phe Phe His Arg Leu Glu Glu Ser Phe Leu Val Glu Glu Asp Lys Lys 
            100                 105                 110         


His Glu Arg His Pro Ile Phe Gly Asn Ile Val Asp Glu Val Ala Tyr 
        115                 120                 125             


His Glu Lys Tyr Pro Thr Ile Tyr His Leu Arg Lys Lys Leu Val Asp 
    130                 135                 140                 


Ser Thr Asp Lys Ala Asp Leu Arg Leu Ile Tyr Leu Ala Leu Ala His 
145                 150                 155                 160 


Met Ile Lys Phe Arg Gly His Phe Leu Ile Glu Gly Asp Leu Asn Pro 
                165                 170                 175     


Asp Asn Ser Asp Val Asp Lys Leu Phe Ile Gln Leu Val Gln Thr Tyr 
            180                 185                 190         


Asn Gln Leu Phe Glu Glu Asn Pro Ile Asn Ala Ser Gly Val Asp Ala 
        195                 200                 205             


Lys Ala Ile Leu Ser Ala Arg Leu Ser Lys Ser Arg Arg Leu Glu Asn 
    210                 215                 220                 


Leu Ile Ala Gln Leu Pro Gly Glu Lys Lys Asn Gly Leu Phe Gly Asn 
225                 230                 235                 240 


Leu Ile Ala Leu Ser Leu Gly Leu Thr Pro Asn Phe Lys Ser Asn Phe 
                245                 250                 255     


Asp Leu Ala Glu Asp Ala Lys Leu Gln Leu Ser Lys Asp Thr Tyr Asp 
            260                 265                 270         


Asp Asp Leu Asp Asn Leu Leu Ala Gln Ile Gly Asp Gln Tyr Ala Asp 
        275                 280                 285             


Leu Phe Leu Ala Ala Lys Asn Leu Ser Asp Ala Ile Leu Leu Ser Asp 
    290                 295                 300                 


Ile Leu Arg Val Asn Thr Glu Ile Thr Lys Ala Pro Leu Ser Ala Ser 
305                 310                 315                 320 


Met Ile Lys Arg Tyr Asp Glu His His Gln Asp Leu Thr Leu Leu Lys 
                325                 330                 335     


Ala Leu Val Arg Gln Gln Leu Pro Glu Lys Tyr Lys Glu Ile Phe Phe 
            340                 345                 350         


Asp Gln Ser Lys Asn Gly Tyr Ala Gly Tyr Ile Asp Gly Gly Ala Ser 
        355                 360                 365             


Gln Glu Glu Phe Tyr Lys Phe Ile Lys Pro Ile Leu Glu Lys Met Asp 
    370                 375                 380                 


Gly Thr Glu Glu Leu Leu Val Lys Leu Asn Arg Glu Asp Leu Leu Arg 
385                 390                 395                 400 


Lys Gln Arg Thr Phe Asp Asn Gly Ser Ile Pro His Gln Ile His Leu 
                405                 410                 415     


Gly Glu Leu His Ala Ile Leu Arg Arg Gln Glu Asp Phe Tyr Pro Phe 
            420                 425                 430         


Leu Lys Asp Asn Arg Glu Lys Ile Glu Lys Ile Leu Thr Phe Arg Ile 
        435                 440                 445             


Pro Tyr Tyr Val Gly Pro Leu Ala Arg Gly Asn Ser Arg Phe Ala Trp 
    450                 455                 460                 


Met Thr Arg Lys Ser Glu Glu Thr Ile Thr Pro Trp Asn Phe Glu Glu 
465                 470                 475                 480 


Val Val Asp Lys Gly Ala Ser Ala Gln Ser Phe Ile Glu Arg Met Thr 
                485                 490                 495     


Asn Phe Asp Lys Asn Leu Pro Asn Glu Lys Val Leu Pro Lys His Ser 
            500                 505                 510         


Leu Leu Tyr Glu Tyr Phe Thr Val Tyr Asn Glu Leu Thr Lys Val Lys 
        515                 520                 525             


Tyr Val Thr Glu Gly Met Arg Lys Pro Ala Phe Leu Ser Gly Glu Gln 
    530                 535                 540                 


Lys Lys Ala Ile Val Asp Leu Leu Phe Lys Thr Asn Arg Lys Val Thr 
545                 550                 555                 560 


Val Lys Gln Leu Lys Glu Asp Tyr Phe Lys Lys Ile Glu Cys Phe Asp 
                565                 570                 575     


Ser Val Glu Ile Ser Gly Val Glu Asp Arg Phe Asn Ala Ser Leu Gly 
            580                 585                 590         


Thr Tyr His Asp Leu Leu Lys Ile Ile Lys Asp Lys Asp Phe Leu Asp 
        595                 600                 605             


Asn Glu Glu Asn Glu Asp Ile Leu Glu Asp Ile Val Leu Thr Leu Thr 
    610                 615                 620                 


Leu Phe Glu Asp Arg Glu Met Ile Glu Glu Arg Leu Lys Thr Tyr Ala 
625                 630                 635                 640 


His Leu Phe Asp Asp Lys Val Met Lys Gln Leu Lys Arg Arg Arg Tyr 
                645                 650                 655     


Thr Gly Trp Gly Arg Leu Ser Arg Lys Leu Ile Asn Gly Ile Arg Asp 
            660                 665                 670         


Lys Gln Ser Gly Lys Thr Ile Leu Asp Phe Leu Lys Ser Asp Gly Phe 
        675                 680                 685             


Ala Asn Arg Asn Phe Met Gln Leu Ile His Asp Asp Ser Leu Thr Phe 
    690                 695                 700                 


Lys Glu Asp Ile Gln Lys Ala Gln Val Ser Gly Gln Gly Asp Ser Leu 
705                 710                 715                 720 


His Glu His Ile Ala Asn Leu Ala Gly Ser Pro Ala Ile Lys Lys Gly 
                725                 730                 735     


Ile Leu Gln Thr Val Lys Val Val Asp Glu Leu Val Lys Val Met Gly 
            740                 745                 750         


Arg His Lys Pro Glu Asn Ile Val Ile Glu Met Ala Arg Glu Asn Gln 
        755                 760                 765             


Thr Thr Gln Lys Gly Gln Lys Asn Ser Arg Glu Arg Met Lys Arg Ile 
    770                 775                 780                 


Glu Glu Gly Ile Lys Glu Leu Gly Ser Gln Ile Leu Lys Glu His Pro 
785                 790                 795                 800 


Val Glu Asn Thr Gln Leu Gln Asn Glu Lys Leu Tyr Leu Tyr Tyr Leu 
                805                 810                 815     


Gln Asn Gly Arg Asp Met Tyr Val Asp Gln Glu Leu Asp Ile Asn Arg 
            820                 825                 830         


Leu Ser Asp Tyr Asp Val Asp Ala Ile Val Pro Gln Ser Phe Leu Lys 
        835                 840                 845             


Asp Asp Ser Ile Asp Asn Lys Val Leu Thr Arg Ser Asp Lys Asn Arg 
    850                 855                 860                 


Gly Lys Ser Asp Asn Val Pro Ser Glu Glu Val Val Lys Lys Met Lys 
865                 870                 875                 880 


Asn Tyr Trp Arg Gln Leu Leu Asn Ala Lys Leu Ile Thr Gln Arg Lys 
                885                 890                 895     


Phe Asp Asn Leu Thr Lys Ala Glu Arg Gly Gly Leu Ser Glu Leu Asp 
            900                 905                 910         


Lys Ala Gly Phe Ile Lys Arg Gln Leu Val Glu Thr Arg Gln Ile Thr 
        915                 920                 925             


Lys His Val Ala Gln Ile Leu Asp Ser Arg Met Asn Thr Lys Tyr Asp 
    930                 935                 940                 


Glu Asn Asp Lys Leu Ile Arg Glu Val Lys Val Ile Thr Leu Lys Ser 
945                 950                 955                 960 


Lys Leu Val Ser Asp Phe Arg Lys Asp Phe Gln Phe Tyr Lys Val Arg 
                965                 970                 975     


Glu Ile Asn Asn Tyr His His Ala His Asp Ala Tyr Leu Asn Ala Val 
            980                 985                 990         


Val Gly Thr Ala Leu Ile Lys Lys  Tyr Pro Lys Leu Glu  Ser Glu Phe 
        995                 1000                 1005             


Val Tyr  Gly Asp Tyr Lys Val  Tyr Asp Val Arg Lys  Met Ile Ala 
    1010                 1015                 1020             


Lys Ser  Glu Gln Glu Ile Gly  Lys Ala Thr Ala Lys  Tyr Phe Phe 
    1025                 1030                 1035             


Tyr Ser  Asn Ile Met Asn Phe  Phe Lys Thr Glu Ile  Thr Leu Ala 
    1040                 1045                 1050             


Asn Gly  Glu Ile Arg Lys Arg  Pro Leu Ile Glu Thr  Asn Gly Glu 
    1055                 1060                 1065             


Thr Gly  Glu Ile Val Trp Asp  Lys Gly Arg Asp Phe  Ala Thr Val 
    1070                 1075                 1080             


Arg Lys  Val Leu Ser Met Pro  Gln Val Asn Ile Val  Lys Lys Thr 
    1085                 1090                 1095             


Glu Val  Gln Thr Gly Gly Phe  Ser Lys Glu Ser Ile  Leu Pro Lys 
    1100                 1105                 1110             


Arg Asn  Ser Asp Lys Leu Ile  Ala Arg Lys Lys Asp  Trp Asp Pro 
    1115                 1120                 1125             


Lys Lys  Tyr Gly Gly Phe Asp  Ser Pro Thr Val Ala  Tyr Ser Val 
    1130                 1135                 1140             


Leu Val  Val Ala Lys Val Glu  Lys Gly Lys Ser Lys  Lys Leu Lys 
    1145                 1150                 1155             


Ser Val  Lys Glu Leu Leu Gly  Ile Thr Ile Met Glu  Arg Ser Ser 
    1160                 1165                 1170             


Phe Glu  Lys Asn Pro Ile Asp  Phe Leu Glu Ala Lys  Gly Tyr Lys 
    1175                 1180                 1185             


Glu Val  Lys Lys Asp Leu Ile  Ile Lys Leu Pro Lys  Tyr Ser Leu 
    1190                 1195                 1200             


Phe Glu  Leu Glu Asn Gly Arg  Lys Arg Met Leu Ala  Ser Ala Gly 
    1205                 1210                 1215             


Glu Leu  Gln Lys Gly Asn Glu  Leu Ala Leu Pro Ser  Lys Tyr Val 
    1220                 1225                 1230             


Asn Phe  Leu Tyr Leu Ala Ser  His Tyr Glu Lys Leu  Lys Gly Ser 
    1235                 1240                 1245             


Pro Glu  Asp Asn Glu Gln Lys  Gln Leu Phe Val Glu  Gln His Lys 
    1250                 1255                 1260             


His Tyr  Leu Asp Glu Ile Ile  Glu Gln Ile Ser Glu  Phe Ser Lys 
    1265                 1270                 1275             


Arg Val  Ile Leu Ala Asp Ala  Asn Leu Asp Lys Val  Leu Ser Ala 
    1280                 1285                 1290             


Tyr Asn  Lys His Arg Asp Lys  Pro Ile Arg Glu Gln  Ala Glu Asn 
    1295                 1300                 1305             


Ile Ile  His Leu Phe Thr Leu  Thr Asn Leu Gly Ala  Pro Ala Ala 
    1310                 1315                 1320             


Phe Lys  Tyr Phe Asp Thr Thr  Ile Asp Arg Lys Arg  Tyr Thr Ser 
    1325                 1330                 1335             


Thr Lys  Glu Val Leu Asp Ala  Thr Leu Ile His Gln  Ser Ile Thr 
    1340                 1345                 1350             


Gly Leu  Tyr Glu Thr Arg Ile  Asp Leu Ser Gln Leu  Gly Gly Asp 
    1355                 1360                 1365             


Glu Gly  Ala Asp Pro Lys Lys  Lys Arg Lys Val Asp  Pro Lys Lys 
    1370                 1375                 1380             


Lys Arg  Lys Val Asp Pro Lys  Lys Lys Arg Lys Val  Gly Ser Met 
    1385                 1390                 1395             


Pro Ala  Met Pro Ser Ser Gly  Pro Gly Asp Thr Ser  Ser Ser Ala 
    1400                 1405                 1410             


Ala Glu  Arg Glu Glu Asp Arg  Lys Asp Gly Glu Glu  Gln Glu Glu 
    1415                 1420                 1425             


Pro Arg  Gly Lys Glu Glu Arg  Gln Glu Pro Ser Thr  Thr Ala Arg 
    1430                 1435                 1440             


Lys Val  Gly Arg Pro Gly Arg  Lys Arg Lys His Pro  Pro Val Glu 
    1445                 1450                 1455             


Ser Gly  Asp Thr Pro Lys Asp  Pro Ala Val Ile Ser  Lys Ser Pro 
    1460                 1465                 1470             


Ser Met  Ala Gln Asp Ser Gly  Ala Ser Glu Leu Leu  Pro Asn Gly 
    1475                 1480                 1485             


Asp Leu  Glu Lys Arg Ser Glu  Pro Gln Pro Glu Glu  Gly Ser Pro 
    1490                 1495                 1500             


Ala Gly  Gly Gln Lys Gly Gly  Ala Pro Ala Glu Gly  Glu Gly Ala 
    1505                 1510                 1515             


Ala Glu  Thr Leu Pro Glu Ala  Ser Arg Ala Val Glu  Asn Gly Cys 
    1520                 1525                 1530             


Cys Thr  Pro Lys Glu Gly Arg  Gly Ala Pro Ala Glu  Ala Gly Lys 
    1535                 1540                 1545             


Glu Gln  Lys Glu Thr Asn Ile  Glu Ser Met Lys Met  Glu Gly Ser 
    1550                 1555                 1560             


Arg Gly  Arg Leu Arg Gly Gly  Leu Gly Trp Glu Ser  Ser Leu Arg 
    1565                 1570                 1575             


Gln Arg  Pro Met Pro Arg Leu  Thr Phe Gln Ala Gly  Asp Pro Tyr 
    1580                 1585                 1590             


Tyr Ile  Ser Lys Arg Lys Arg  Asp Glu Trp Leu Ala  Arg Trp Lys 
    1595                 1600                 1605             


Arg Glu  Ala Glu Lys Lys Ala  Lys Val Ile Ala Gly  Met Asn Ala 
    1610                 1615                 1620             


Val Glu  Glu Asn Gln Gly Pro  Gly Glu Ser Gln Lys  Val Glu Glu 
    1625                 1630                 1635             


Ala Ser  Pro Pro Ala Val Gln  Gln Pro Thr Asp Pro  Ala Ser Pro 
    1640                 1645                 1650             


Thr Val  Ala Thr Thr Pro Glu  Pro Val Gly Ser Asp  Ala Gly Asp 
    1655                 1660                 1665             


Lys Asn  Ala Thr Lys Ala Gly  Asp Asp Glu Pro Glu  Tyr Glu Asp 
    1670                 1675                 1680             


Gly Arg  Gly Phe Gly Ile Gly  Glu Leu Val Trp Gly  Lys Leu Arg 
    1685                 1690                 1695             


Gly Phe  Ser Trp Trp Pro Gly  Arg Ile Val Ser Trp  Trp Met Thr 
    1700                 1705                 1710             


Gly Arg  Ser Arg Ala Ala Glu  Gly Thr Arg Trp Val  Met Trp Phe 
    1715                 1720                 1725             


Gly Asp  Gly Lys Phe Ser Val  Val Cys Val Glu Lys  Leu Met Pro 
    1730                 1735                 1740             


Leu Ser  Ser Phe Cys Ser Ala  Phe His Gln Ala Thr  Tyr Asn Lys 
    1745                 1750                 1755             


Gln Pro  Met Tyr Arg Lys Ala  Ile Tyr Glu Val Leu  Gln Val Ala 
    1760                 1765                 1770             


Ser Ser  Arg Ala Gly Lys Leu  Phe Pro Val Cys His  Asp Ser Asp 
    1775                 1780                 1785             


Glu Ser  Asp Thr Ala Lys Ala  Val Glu Val Gln Asn  Lys Pro Met 
    1790                 1795                 1800             


Ile Glu  Trp Ala Leu Gly Gly  Phe Gln Pro Ser Gly  Pro Lys Gly 
    1805                 1810                 1815             


Leu Glu  Pro Pro Glu Glu Glu  Lys Asn Pro Tyr Lys  Glu Val Tyr 
    1820                 1825                 1830             


Thr Asp  Met Trp Val Glu Pro  Glu Ala Ala Ala Tyr  Ala Pro Pro 
    1835                 1840                 1845             


Pro Pro  Ala Lys Lys Pro Arg  Lys Ser Thr Ala Glu  Lys Pro Lys 
    1850                 1855                 1860             


Val Lys  Glu Ile Ile Asp Glu  Arg Thr Arg Glu Arg  Leu Val Tyr 
    1865                 1870                 1875             


Glu Val  Arg Gln Lys Cys Arg  Asn Ile Glu Asp Ile  Cys Ile Ser 
    1880                 1885                 1890             


Cys Gly  Ser Leu Asn Val Thr  Leu Glu His Pro Leu  Phe Val Gly 
    1895                 1900                 1905             


Gly Met  Cys Gln Asn Cys Lys  Asn Cys Phe Leu Glu  Cys Ala Tyr 
    1910                 1915                 1920             


Gln Tyr  Asp Asp Asp Gly Tyr  Gln Ser Tyr Cys Thr  Ile Cys Cys 
    1925                 1930                 1935             


Gly Gly  Arg Glu Val Leu Met  Cys Gly Asn Asn Asn  Cys Cys Arg 
    1940                 1945                 1950             


Cys Phe  Cys Val Glu Cys Val  Asp Leu Leu Val Gly  Pro Gly Ala 
    1955                 1960                 1965             


Ala Gln  Ala Ala Ile Lys Glu  Asp Pro Trp Asn Cys  Tyr Met Cys 
    1970                 1975                 1980             


Gly His  Lys Gly Thr Tyr Gly  Leu Leu Arg Arg Arg  Glu Asp Trp 
    1985                 1990                 1995             


Pro Ser  Arg Leu Gln Met Phe  Phe Ala Asn Asn His  Asp Gln Glu 
    2000                 2005                 2010             


Phe Asp  Pro Pro Lys Val Tyr  Pro Pro Val Pro Ala  Glu Lys Arg 
    2015                 2020                 2025             


Lys Pro  Ile Arg Val Leu Ser  Leu Phe Asp Gly Ile  Ala Thr Gly 
    2030                 2035                 2040             


Leu Leu  Val Leu Lys Asp Leu  Gly Ile Gln Val Asp  Arg Tyr Ile 
    2045                 2050                 2055             


Ala Ser  Glu Val Cys Glu Asp  Ser Ile Thr Val Gly  Met Val Arg 
    2060                 2065                 2070             


His Gln  Gly Lys Ile Met Tyr  Val Gly Asp Val Arg  Ser Val Thr 
    2075                 2080                 2085             


Gln Lys  His Ile Gln Glu Trp  Gly Pro Phe Asp Leu  Val Ile Gly 
    2090                 2095                 2100             


Gly Ser  Pro Cys Asn Asp Leu  Ser Ile Val Asn Pro  Ala Arg Lys 
    2105                 2110                 2115             


Gly Leu  Tyr Glu Gly Thr Gly  Arg Leu Phe Phe Glu  Phe Tyr Arg 
    2120                 2125                 2130             


Leu Leu  His Asp Ala Arg Pro  Lys Glu Gly Asp Asp  Arg Pro Phe 
    2135                 2140                 2145             


Phe Trp  Leu Phe Glu Asn Val  Val Ala Met Gly Val  Ser Asp Lys 
    2150                 2155                 2160             


Arg Asp  Ile Ser Arg Phe Leu  Glu Ser Asn Pro Val  Met Ile Asp 
    2165                 2170                 2175             


Ala Lys  Glu Val Ser Ala Ala  His Arg Ala Arg Tyr  Phe Trp Gly 
    2180                 2185                 2190             


Asn Leu  Pro Gly Met Asn Arg  Pro Leu Ala Ser Thr  Val Asn Asp 
    2195                 2200                 2205             


Lys Leu  Glu Leu Gln Glu Cys  Leu Glu His Gly Arg  Ile Ala Lys 
    2210                 2215                 2220             


Phe Ser  Lys Val Arg Thr Ile  Thr Thr Arg Ser Asn  Ser Ile Lys 
    2225                 2230                 2235             


Gln Gly  Lys Asp Gln His Phe  Pro Val Phe Met Asn  Glu Lys Glu 
    2240                 2245                 2250             


Asp Ile  Leu Trp Cys Thr Glu  Met Glu Arg Val Phe  Gly Phe Pro 
    2255                 2260                 2265             


Val His  Tyr Thr Asp Val Ser  Asn Met Ser Arg Leu  Ala Arg Gln 
    2270                 2275                 2280             


Arg Leu  Leu Gly Arg Ser Trp  Ser Val Pro Val Ile  Arg His Leu 
    2285                 2290                 2295             


Phe Ala  Pro Leu Lys Glu Tyr  Phe Ala Cys Val 
    2300                 2305                 


<210>  158
<211>  2309
<212>  PRT
<213>  Unknown

<220>
<223>  dCAS9-Dnmt3a_IM

<400>  158

Met Asp Lys Lys Tyr Ser Ile Gly Leu Ala Ile Gly Thr Asn Ser Val 
1               5                   10                  15      


Gly Trp Ala Val Ile Thr Asp Glu Tyr Lys Val Pro Ser Lys Lys Phe 
            20                  25                  30          


Lys Val Leu Gly Asn Thr Asp Arg His Ser Ile Lys Lys Asn Leu Ile 
        35                  40                  45              


Gly Ala Leu Leu Phe Asp Ser Gly Glu Thr Ala Glu Ala Thr Arg Leu 
    50                  55                  60                  


Lys Arg Thr Ala Arg Arg Arg Tyr Thr Arg Arg Lys Asn Arg Ile Cys 
65                  70                  75                  80  


Tyr Leu Gln Glu Ile Phe Ser Asn Glu Met Ala Lys Val Asp Asp Ser 
                85                  90                  95      


Phe Phe His Arg Leu Glu Glu Ser Phe Leu Val Glu Glu Asp Lys Lys 
            100                 105                 110         


His Glu Arg His Pro Ile Phe Gly Asn Ile Val Asp Glu Val Ala Tyr 
        115                 120                 125             


His Glu Lys Tyr Pro Thr Ile Tyr His Leu Arg Lys Lys Leu Val Asp 
    130                 135                 140                 


Ser Thr Asp Lys Ala Asp Leu Arg Leu Ile Tyr Leu Ala Leu Ala His 
145                 150                 155                 160 


Met Ile Lys Phe Arg Gly His Phe Leu Ile Glu Gly Asp Leu Asn Pro 
                165                 170                 175     


Asp Asn Ser Asp Val Asp Lys Leu Phe Ile Gln Leu Val Gln Thr Tyr 
            180                 185                 190         


Asn Gln Leu Phe Glu Glu Asn Pro Ile Asn Ala Ser Gly Val Asp Ala 
        195                 200                 205             


Lys Ala Ile Leu Ser Ala Arg Leu Ser Lys Ser Arg Arg Leu Glu Asn 
    210                 215                 220                 


Leu Ile Ala Gln Leu Pro Gly Glu Lys Lys Asn Gly Leu Phe Gly Asn 
225                 230                 235                 240 


Leu Ile Ala Leu Ser Leu Gly Leu Thr Pro Asn Phe Lys Ser Asn Phe 
                245                 250                 255     


Asp Leu Ala Glu Asp Ala Lys Leu Gln Leu Ser Lys Asp Thr Tyr Asp 
            260                 265                 270         


Asp Asp Leu Asp Asn Leu Leu Ala Gln Ile Gly Asp Gln Tyr Ala Asp 
        275                 280                 285             


Leu Phe Leu Ala Ala Lys Asn Leu Ser Asp Ala Ile Leu Leu Ser Asp 
    290                 295                 300                 


Ile Leu Arg Val Asn Thr Glu Ile Thr Lys Ala Pro Leu Ser Ala Ser 
305                 310                 315                 320 


Met Ile Lys Arg Tyr Asp Glu His His Gln Asp Leu Thr Leu Leu Lys 
                325                 330                 335     


Ala Leu Val Arg Gln Gln Leu Pro Glu Lys Tyr Lys Glu Ile Phe Phe 
            340                 345                 350         


Asp Gln Ser Lys Asn Gly Tyr Ala Gly Tyr Ile Asp Gly Gly Ala Ser 
        355                 360                 365             


Gln Glu Glu Phe Tyr Lys Phe Ile Lys Pro Ile Leu Glu Lys Met Asp 
    370                 375                 380                 


Gly Thr Glu Glu Leu Leu Val Lys Leu Asn Arg Glu Asp Leu Leu Arg 
385                 390                 395                 400 


Lys Gln Arg Thr Phe Asp Asn Gly Ser Ile Pro His Gln Ile His Leu 
                405                 410                 415     


Gly Glu Leu His Ala Ile Leu Arg Arg Gln Glu Asp Phe Tyr Pro Phe 
            420                 425                 430         


Leu Lys Asp Asn Arg Glu Lys Ile Glu Lys Ile Leu Thr Phe Arg Ile 
        435                 440                 445             


Pro Tyr Tyr Val Gly Pro Leu Ala Arg Gly Asn Ser Arg Phe Ala Trp 
    450                 455                 460                 


Met Thr Arg Lys Ser Glu Glu Thr Ile Thr Pro Trp Asn Phe Glu Glu 
465                 470                 475                 480 


Val Val Asp Lys Gly Ala Ser Ala Gln Ser Phe Ile Glu Arg Met Thr 
                485                 490                 495     


Asn Phe Asp Lys Asn Leu Pro Asn Glu Lys Val Leu Pro Lys His Ser 
            500                 505                 510         


Leu Leu Tyr Glu Tyr Phe Thr Val Tyr Asn Glu Leu Thr Lys Val Lys 
        515                 520                 525             


Tyr Val Thr Glu Gly Met Arg Lys Pro Ala Phe Leu Ser Gly Glu Gln 
    530                 535                 540                 


Lys Lys Ala Ile Val Asp Leu Leu Phe Lys Thr Asn Arg Lys Val Thr 
545                 550                 555                 560 


Val Lys Gln Leu Lys Glu Asp Tyr Phe Lys Lys Ile Glu Cys Phe Asp 
                565                 570                 575     


Ser Val Glu Ile Ser Gly Val Glu Asp Arg Phe Asn Ala Ser Leu Gly 
            580                 585                 590         


Thr Tyr His Asp Leu Leu Lys Ile Ile Lys Asp Lys Asp Phe Leu Asp 
        595                 600                 605             


Asn Glu Glu Asn Glu Asp Ile Leu Glu Asp Ile Val Leu Thr Leu Thr 
    610                 615                 620                 


Leu Phe Glu Asp Arg Glu Met Ile Glu Glu Arg Leu Lys Thr Tyr Ala 
625                 630                 635                 640 


His Leu Phe Asp Asp Lys Val Met Lys Gln Leu Lys Arg Arg Arg Tyr 
                645                 650                 655     


Thr Gly Trp Gly Arg Leu Ser Arg Lys Leu Ile Asn Gly Ile Arg Asp 
            660                 665                 670         


Lys Gln Ser Gly Lys Thr Ile Leu Asp Phe Leu Lys Ser Asp Gly Phe 
        675                 680                 685             


Ala Asn Arg Asn Phe Met Gln Leu Ile His Asp Asp Ser Leu Thr Phe 
    690                 695                 700                 


Lys Glu Asp Ile Gln Lys Ala Gln Val Ser Gly Gln Gly Asp Ser Leu 
705                 710                 715                 720 


His Glu His Ile Ala Asn Leu Ala Gly Ser Pro Ala Ile Lys Lys Gly 
                725                 730                 735     


Ile Leu Gln Thr Val Lys Val Val Asp Glu Leu Val Lys Val Met Gly 
            740                 745                 750         


Arg His Lys Pro Glu Asn Ile Val Ile Glu Met Ala Arg Glu Asn Gln 
        755                 760                 765             


Thr Thr Gln Lys Gly Gln Lys Asn Ser Arg Glu Arg Met Lys Arg Ile 
    770                 775                 780                 


Glu Glu Gly Ile Lys Glu Leu Gly Ser Gln Ile Leu Lys Glu His Pro 
785                 790                 795                 800 


Val Glu Asn Thr Gln Leu Gln Asn Glu Lys Leu Tyr Leu Tyr Tyr Leu 
                805                 810                 815     


Gln Asn Gly Arg Asp Met Tyr Val Asp Gln Glu Leu Asp Ile Asn Arg 
            820                 825                 830         


Leu Ser Asp Tyr Asp Val Asp Ala Ile Val Pro Gln Ser Phe Leu Lys 
        835                 840                 845             


Asp Asp Ser Ile Asp Asn Lys Val Leu Thr Arg Ser Asp Lys Asn Arg 
    850                 855                 860                 


Gly Lys Ser Asp Asn Val Pro Ser Glu Glu Val Val Lys Lys Met Lys 
865                 870                 875                 880 


Asn Tyr Trp Arg Gln Leu Leu Asn Ala Lys Leu Ile Thr Gln Arg Lys 
                885                 890                 895     


Phe Asp Asn Leu Thr Lys Ala Glu Arg Gly Gly Leu Ser Glu Leu Asp 
            900                 905                 910         


Lys Ala Gly Phe Ile Lys Arg Gln Leu Val Glu Thr Arg Gln Ile Thr 
        915                 920                 925             


Lys His Val Ala Gln Ile Leu Asp Ser Arg Met Asn Thr Lys Tyr Asp 
    930                 935                 940                 


Glu Asn Asp Lys Leu Ile Arg Glu Val Lys Val Ile Thr Leu Lys Ser 
945                 950                 955                 960 


Lys Leu Val Ser Asp Phe Arg Lys Asp Phe Gln Phe Tyr Lys Val Arg 
                965                 970                 975     


Glu Ile Asn Asn Tyr His His Ala His Asp Ala Tyr Leu Asn Ala Val 
            980                 985                 990         


Val Gly Thr Ala Leu Ile Lys Lys  Tyr Pro Lys Leu Glu  Ser Glu Phe 
        995                 1000                 1005             


Val Tyr  Gly Asp Tyr Lys Val  Tyr Asp Val Arg Lys  Met Ile Ala 
    1010                 1015                 1020             


Lys Ser  Glu Gln Glu Ile Gly  Lys Ala Thr Ala Lys  Tyr Phe Phe 
    1025                 1030                 1035             


Tyr Ser  Asn Ile Met Asn Phe  Phe Lys Thr Glu Ile  Thr Leu Ala 
    1040                 1045                 1050             


Asn Gly  Glu Ile Arg Lys Arg  Pro Leu Ile Glu Thr  Asn Gly Glu 
    1055                 1060                 1065             


Thr Gly  Glu Ile Val Trp Asp  Lys Gly Arg Asp Phe  Ala Thr Val 
    1070                 1075                 1080             


Arg Lys  Val Leu Ser Met Pro  Gln Val Asn Ile Val  Lys Lys Thr 
    1085                 1090                 1095             


Glu Val  Gln Thr Gly Gly Phe  Ser Lys Glu Ser Ile  Leu Pro Lys 
    1100                 1105                 1110             


Arg Asn  Ser Asp Lys Leu Ile  Ala Arg Lys Lys Asp  Trp Asp Pro 
    1115                 1120                 1125             


Lys Lys  Tyr Gly Gly Phe Asp  Ser Pro Thr Val Ala  Tyr Ser Val 
    1130                 1135                 1140             


Leu Val  Val Ala Lys Val Glu  Lys Gly Lys Ser Lys  Lys Leu Lys 
    1145                 1150                 1155             


Ser Val  Lys Glu Leu Leu Gly  Ile Thr Ile Met Glu  Arg Ser Ser 
    1160                 1165                 1170             


Phe Glu  Lys Asn Pro Ile Asp  Phe Leu Glu Ala Lys  Gly Tyr Lys 
    1175                 1180                 1185             


Glu Val  Lys Lys Asp Leu Ile  Ile Lys Leu Pro Lys  Tyr Ser Leu 
    1190                 1195                 1200             


Phe Glu  Leu Glu Asn Gly Arg  Lys Arg Met Leu Ala  Ser Ala Gly 
    1205                 1210                 1215             


Glu Leu  Gln Lys Gly Asn Glu  Leu Ala Leu Pro Ser  Lys Tyr Val 
    1220                 1225                 1230             


Asn Phe  Leu Tyr Leu Ala Ser  His Tyr Glu Lys Leu  Lys Gly Ser 
    1235                 1240                 1245             


Pro Glu  Asp Asn Glu Gln Lys  Gln Leu Phe Val Glu  Gln His Lys 
    1250                 1255                 1260             


His Tyr  Leu Asp Glu Ile Ile  Glu Gln Ile Ser Glu  Phe Ser Lys 
    1265                 1270                 1275             


Arg Val  Ile Leu Ala Asp Ala  Asn Leu Asp Lys Val  Leu Ser Ala 
    1280                 1285                 1290             


Tyr Asn  Lys His Arg Asp Lys  Pro Ile Arg Glu Gln  Ala Glu Asn 
    1295                 1300                 1305             


Ile Ile  His Leu Phe Thr Leu  Thr Asn Leu Gly Ala  Pro Ala Ala 
    1310                 1315                 1320             


Phe Lys  Tyr Phe Asp Thr Thr  Ile Asp Arg Lys Arg  Tyr Thr Ser 
    1325                 1330                 1335             


Thr Lys  Glu Val Leu Asp Ala  Thr Leu Ile His Gln  Ser Ile Thr 
    1340                 1345                 1350             


Gly Leu  Tyr Glu Thr Arg Ile  Asp Leu Ser Gln Leu  Gly Gly Asp 
    1355                 1360                 1365             


Glu Gly  Ala Asp Pro Lys Lys  Lys Arg Lys Val Asp  Pro Lys Lys 
    1370                 1375                 1380             


Lys Arg  Lys Val Asp Pro Lys  Lys Lys Arg Lys Val  Gly Ser Met 
    1385                 1390                 1395             


Pro Ala  Met Pro Ser Ser Gly  Pro Gly Asp Thr Ser  Ser Ser Ala 
    1400                 1405                 1410             


Ala Glu  Arg Glu Glu Asp Arg  Lys Asp Gly Glu Glu  Gln Glu Glu 
    1415                 1420                 1425             


Pro Arg  Gly Lys Glu Glu Arg  Gln Glu Pro Ser Thr  Thr Ala Arg 
    1430                 1435                 1440             


Lys Val  Gly Arg Pro Gly Arg  Lys Arg Lys His Pro  Pro Val Glu 
    1445                 1450                 1455             


Ser Gly  Asp Thr Pro Lys Asp  Pro Ala Val Ile Ser  Lys Ser Pro 
    1460                 1465                 1470             


Ser Met  Ala Gln Asp Ser Gly  Ala Ser Glu Leu Leu  Pro Asn Gly 
    1475                 1480                 1485             


Asp Leu  Glu Lys Arg Ser Glu  Pro Gln Pro Glu Glu  Gly Ser Pro 
    1490                 1495                 1500             


Ala Gly  Gly Gln Lys Gly Gly  Ala Pro Ala Glu Gly  Glu Gly Ala 
    1505                 1510                 1515             


Ala Glu  Thr Leu Pro Glu Ala  Ser Arg Ala Val Glu  Asn Gly Cys 
    1520                 1525                 1530             


Cys Thr  Pro Lys Glu Gly Arg  Gly Ala Pro Ala Glu  Ala Gly Lys 
    1535                 1540                 1545             


Glu Gln  Lys Glu Thr Asn Ile  Glu Ser Met Lys Met  Glu Gly Ser 
    1550                 1555                 1560             


Arg Gly  Arg Leu Arg Gly Gly  Leu Gly Trp Glu Ser  Ser Leu Arg 
    1565                 1570                 1575             


Gln Arg  Pro Met Pro Arg Leu  Thr Phe Gln Ala Gly  Asp Pro Tyr 
    1580                 1585                 1590             


Tyr Ile  Ser Lys Arg Lys Arg  Asp Glu Trp Leu Ala  Arg Trp Lys 
    1595                 1600                 1605             


Arg Glu  Ala Glu Lys Lys Ala  Lys Val Ile Ala Gly  Met Asn Ala 
    1610                 1615                 1620             


Val Glu  Glu Asn Gln Gly Pro  Gly Glu Ser Gln Lys  Val Glu Glu 
    1625                 1630                 1635             


Ala Ser  Pro Pro Ala Val Gln  Gln Pro Thr Asp Pro  Ala Ser Pro 
    1640                 1645                 1650             


Thr Val  Ala Thr Thr Pro Glu  Pro Val Gly Ser Asp  Ala Gly Asp 
    1655                 1660                 1665             


Lys Asn  Ala Thr Lys Ala Gly  Asp Asp Glu Pro Glu  Tyr Glu Asp 
    1670                 1675                 1680             


Gly Arg  Gly Phe Gly Ile Gly  Glu Leu Val Trp Gly  Lys Leu Arg 
    1685                 1690                 1695             


Gly Phe  Ser Trp Trp Pro Gly  Arg Ile Val Ser Trp  Trp Met Thr 
    1700                 1705                 1710             


Gly Arg  Ser Arg Ala Ala Glu  Gly Thr Arg Trp Val  Met Trp Phe 
    1715                 1720                 1725             


Gly Asp  Gly Lys Phe Ser Val  Val Cys Val Glu Lys  Leu Met Pro 
    1730                 1735                 1740             


Leu Ser  Ser Phe Cys Ser Ala  Phe His Gln Ala Thr  Tyr Asn Lys 
    1745                 1750                 1755             


Gln Pro  Met Tyr Arg Lys Ala  Ile Tyr Glu Val Leu  Gln Val Ala 
    1760                 1765                 1770             


Ser Ser  Arg Ala Gly Lys Leu  Phe Pro Val Cys His  Asp Ser Asp 
    1775                 1780                 1785             


Glu Ser  Asp Thr Ala Lys Ala  Val Glu Val Gln Asn  Lys Pro Met 
    1790                 1795                 1800             


Ile Glu  Trp Ala Leu Gly Gly  Phe Gln Pro Ser Gly  Pro Lys Gly 
    1805                 1810                 1815             


Leu Glu  Pro Pro Glu Glu Glu  Lys Asn Pro Tyr Lys  Glu Val Tyr 
    1820                 1825                 1830             


Thr Asp  Met Trp Val Glu Pro  Glu Ala Ala Ala Tyr  Ala Pro Pro 
    1835                 1840                 1845             


Pro Pro  Ala Lys Lys Pro Arg  Lys Ser Thr Ala Glu  Lys Pro Lys 
    1850                 1855                 1860             


Val Lys  Glu Ile Ile Asp Glu  Arg Thr Arg Glu Arg  Leu Val Tyr 
    1865                 1870                 1875             


Glu Val  Arg Gln Lys Cys Arg  Asn Ile Glu Asp Ile  Cys Ile Ser 
    1880                 1885                 1890             


Cys Gly  Ser Leu Asn Val Thr  Leu Glu His Pro Leu  Phe Val Gly 
    1895                 1900                 1905             


Gly Met  Cys Gln Asn Cys Lys  Asn Cys Phe Leu Glu  Cys Ala Tyr 
    1910                 1915                 1920             


Gln Tyr  Asp Asp Asp Gly Tyr  Gln Ser Tyr Cys Thr  Ile Cys Cys 
    1925                 1930                 1935             


Gly Gly  Arg Glu Val Leu Met  Cys Gly Asn Asn Asn  Cys Cys Arg 
    1940                 1945                 1950             


Cys Phe  Cys Val Glu Cys Val  Asp Leu Leu Val Gly  Pro Gly Ala 
    1955                 1960                 1965             


Ala Gln  Ala Ala Ile Lys Glu  Asp Pro Trp Asn Cys  Tyr Met Cys 
    1970                 1975                 1980             


Gly His  Lys Gly Thr Tyr Gly  Leu Leu Arg Arg Arg  Glu Asp Trp 
    1985                 1990                 1995             


Pro Ser  Arg Leu Gln Met Phe  Phe Ala Asn Asn His  Asp Gln Glu 
    2000                 2005                 2010             


Phe Asp  Pro Pro Lys Val Tyr  Pro Pro Val Pro Ala  Glu Lys Arg 
    2015                 2020                 2025             


Lys Pro  Ile Arg Val Leu Ser  Leu Phe Asp Gly Ile  Ala Thr Gly 
    2030                 2035                 2040             


Leu Leu  Val Leu Lys Asp Leu  Gly Ile Gln Val Asp  Arg Tyr Ile 
    2045                 2050                 2055             


Ala Ser  Ala Val Cys Glu Asp  Ser Ile Thr Val Gly  Met Val Arg 
    2060                 2065                 2070             


His Gln  Gly Lys Ile Met Tyr  Val Gly Asp Val Arg  Ser Val Thr 
    2075                 2080                 2085             


Gln Lys  His Ile Gln Glu Trp  Gly Pro Phe Asp Leu  Val Ile Gly 
    2090                 2095                 2100             


Gly Ser  Pro Cys Asn Asp Leu  Ser Ile Val Asn Pro  Ala Arg Lys 
    2105                 2110                 2115             


Gly Leu  Tyr Glu Gly Thr Gly  Arg Leu Phe Phe Glu  Phe Tyr Arg 
    2120                 2125                 2130             


Leu Leu  His Asp Ala Arg Pro  Lys Glu Gly Asp Asp  Arg Pro Phe 
    2135                 2140                 2145             


Phe Trp  Leu Phe Ala Asn Val  Val Ala Met Gly Val  Ser Asp Lys 
    2150                 2155                 2160             


Arg Asp  Ile Ser Arg Phe Leu  Glu Ser Asn Pro Val  Met Ile Asp 
    2165                 2170                 2175             


Ala Lys  Glu Val Ser Ala Ala  His Arg Ala Arg Tyr  Phe Trp Gly 
    2180                 2185                 2190             


Asn Leu  Pro Gly Met Asn Arg  Pro Leu Ala Ser Thr  Val Asn Asp 
    2195                 2200                 2205             


Lys Leu  Glu Leu Gln Glu Cys  Leu Glu His Gly Arg  Ile Ala Lys 
    2210                 2215                 2220             


Phe Ser  Lys Val Arg Thr Ile  Thr Thr Arg Ser Asn  Ser Ile Lys 
    2225                 2230                 2235             


Gln Gly  Lys Asp Gln His Phe  Pro Val Phe Met Asn  Glu Lys Glu 
    2240                 2245                 2250             


Asp Ile  Leu Trp Cys Thr Glu  Met Glu Arg Val Phe  Gly Phe Pro 
    2255                 2260                 2265             


Val His  Tyr Thr Asp Val Ser  Asn Met Ser Arg Leu  Ala Arg Gln 
    2270                 2275                 2280             


Arg Leu  Leu Gly Arg Ser Trp  Ser Val Pro Val Ile  Arg His Leu 
    2285                 2290                 2295             


Phe Ala  Pro Leu Lys Glu Tyr  Phe Ala Cys Val 
    2300                 2305                 


<210>  159
<211>  2115
<212>  PRT
<213>  Unknown

<220>
<223>  dCAS9-Tet1CD

<400>  159

Met Asp Lys Lys Tyr Ser Ile Gly Leu Ala Ile Gly Thr Asn Ser Val 
1               5                   10                  15      


Gly Trp Ala Val Ile Thr Asp Glu Tyr Lys Val Pro Ser Lys Lys Phe 
            20                  25                  30          


Lys Val Leu Gly Asn Thr Asp Arg His Ser Ile Lys Lys Asn Leu Ile 
        35                  40                  45              


Gly Ala Leu Leu Phe Asp Ser Gly Glu Thr Ala Glu Ala Thr Arg Leu 
    50                  55                  60                  


Lys Arg Thr Ala Arg Arg Arg Tyr Thr Arg Arg Lys Asn Arg Ile Cys 
65                  70                  75                  80  


Tyr Leu Gln Glu Ile Phe Ser Asn Glu Met Ala Lys Val Asp Asp Ser 
                85                  90                  95      


Phe Phe His Arg Leu Glu Glu Ser Phe Leu Val Glu Glu Asp Lys Lys 
            100                 105                 110         


His Glu Arg His Pro Ile Phe Gly Asn Ile Val Asp Glu Val Ala Tyr 
        115                 120                 125             


His Glu Lys Tyr Pro Thr Ile Tyr His Leu Arg Lys Lys Leu Val Asp 
    130                 135                 140                 


Ser Thr Asp Lys Ala Asp Leu Arg Leu Ile Tyr Leu Ala Leu Ala His 
145                 150                 155                 160 


Met Ile Lys Phe Arg Gly His Phe Leu Ile Glu Gly Asp Leu Asn Pro 
                165                 170                 175     


Asp Asn Ser Asp Val Asp Lys Leu Phe Ile Gln Leu Val Gln Thr Tyr 
            180                 185                 190         


Asn Gln Leu Phe Glu Glu Asn Pro Ile Asn Ala Ser Gly Val Asp Ala 
        195                 200                 205             


Lys Ala Ile Leu Ser Ala Arg Leu Ser Lys Ser Arg Arg Leu Glu Asn 
    210                 215                 220                 


Leu Ile Ala Gln Leu Pro Gly Glu Lys Lys Asn Gly Leu Phe Gly Asn 
225                 230                 235                 240 


Leu Ile Ala Leu Ser Leu Gly Leu Thr Pro Asn Phe Lys Ser Asn Phe 
                245                 250                 255     


Asp Leu Ala Glu Asp Ala Lys Leu Gln Leu Ser Lys Asp Thr Tyr Asp 
            260                 265                 270         


Asp Asp Leu Asp Asn Leu Leu Ala Gln Ile Gly Asp Gln Tyr Ala Asp 
        275                 280                 285             


Leu Phe Leu Ala Ala Lys Asn Leu Ser Asp Ala Ile Leu Leu Ser Asp 
    290                 295                 300                 


Ile Leu Arg Val Asn Thr Glu Ile Thr Lys Ala Pro Leu Ser Ala Ser 
305                 310                 315                 320 


Met Ile Lys Arg Tyr Asp Glu His His Gln Asp Leu Thr Leu Leu Lys 
                325                 330                 335     


Ala Leu Val Arg Gln Gln Leu Pro Glu Lys Tyr Lys Glu Ile Phe Phe 
            340                 345                 350         


Asp Gln Ser Lys Asn Gly Tyr Ala Gly Tyr Ile Asp Gly Gly Ala Ser 
        355                 360                 365             


Gln Glu Glu Phe Tyr Lys Phe Ile Lys Pro Ile Leu Glu Lys Met Asp 
    370                 375                 380                 


Gly Thr Glu Glu Leu Leu Val Lys Leu Asn Arg Glu Asp Leu Leu Arg 
385                 390                 395                 400 


Lys Gln Arg Thr Phe Asp Asn Gly Ser Ile Pro His Gln Ile His Leu 
                405                 410                 415     


Gly Glu Leu His Ala Ile Leu Arg Arg Gln Glu Asp Phe Tyr Pro Phe 
            420                 425                 430         


Leu Lys Asp Asn Arg Glu Lys Ile Glu Lys Ile Leu Thr Phe Arg Ile 
        435                 440                 445             


Pro Tyr Tyr Val Gly Pro Leu Ala Arg Gly Asn Ser Arg Phe Ala Trp 
    450                 455                 460                 


Met Thr Arg Lys Ser Glu Glu Thr Ile Thr Pro Trp Asn Phe Glu Glu 
465                 470                 475                 480 


Val Val Asp Lys Gly Ala Ser Ala Gln Ser Phe Ile Glu Arg Met Thr 
                485                 490                 495     


Asn Phe Asp Lys Asn Leu Pro Asn Glu Lys Val Leu Pro Lys His Ser 
            500                 505                 510         


Leu Leu Tyr Glu Tyr Phe Thr Val Tyr Asn Glu Leu Thr Lys Val Lys 
        515                 520                 525             


Tyr Val Thr Glu Gly Met Arg Lys Pro Ala Phe Leu Ser Gly Glu Gln 
    530                 535                 540                 


Lys Lys Ala Ile Val Asp Leu Leu Phe Lys Thr Asn Arg Lys Val Thr 
545                 550                 555                 560 


Val Lys Gln Leu Lys Glu Asp Tyr Phe Lys Lys Ile Glu Cys Phe Asp 
                565                 570                 575     


Ser Val Glu Ile Ser Gly Val Glu Asp Arg Phe Asn Ala Ser Leu Gly 
            580                 585                 590         


Thr Tyr His Asp Leu Leu Lys Ile Ile Lys Asp Lys Asp Phe Leu Asp 
        595                 600                 605             


Asn Glu Glu Asn Glu Asp Ile Leu Glu Asp Ile Val Leu Thr Leu Thr 
    610                 615                 620                 


Leu Phe Glu Asp Arg Glu Met Ile Glu Glu Arg Leu Lys Thr Tyr Ala 
625                 630                 635                 640 


His Leu Phe Asp Asp Lys Val Met Lys Gln Leu Lys Arg Arg Arg Tyr 
                645                 650                 655     


Thr Gly Trp Gly Arg Leu Ser Arg Lys Leu Ile Asn Gly Ile Arg Asp 
            660                 665                 670         


Lys Gln Ser Gly Lys Thr Ile Leu Asp Phe Leu Lys Ser Asp Gly Phe 
        675                 680                 685             


Ala Asn Arg Asn Phe Met Gln Leu Ile His Asp Asp Ser Leu Thr Phe 
    690                 695                 700                 


Lys Glu Asp Ile Gln Lys Ala Gln Val Ser Gly Gln Gly Asp Ser Leu 
705                 710                 715                 720 


His Glu His Ile Ala Asn Leu Ala Gly Ser Pro Ala Ile Lys Lys Gly 
                725                 730                 735     


Ile Leu Gln Thr Val Lys Val Val Asp Glu Leu Val Lys Val Met Gly 
            740                 745                 750         


Arg His Lys Pro Glu Asn Ile Val Ile Glu Met Ala Arg Glu Asn Gln 
        755                 760                 765             


Thr Thr Gln Lys Gly Gln Lys Asn Ser Arg Glu Arg Met Lys Arg Ile 
    770                 775                 780                 


Glu Glu Gly Ile Lys Glu Leu Gly Ser Gln Ile Leu Lys Glu His Pro 
785                 790                 795                 800 


Val Glu Asn Thr Gln Leu Gln Asn Glu Lys Leu Tyr Leu Tyr Tyr Leu 
                805                 810                 815     


Gln Asn Gly Arg Asp Met Tyr Val Asp Gln Glu Leu Asp Ile Asn Arg 
            820                 825                 830         


Leu Ser Asp Tyr Asp Val Asp Ala Ile Val Pro Gln Ser Phe Leu Lys 
        835                 840                 845             


Asp Asp Ser Ile Asp Asn Lys Val Leu Thr Arg Ser Asp Lys Asn Arg 
    850                 855                 860                 


Gly Lys Ser Asp Asn Val Pro Ser Glu Glu Val Val Lys Lys Met Lys 
865                 870                 875                 880 


Asn Tyr Trp Arg Gln Leu Leu Asn Ala Lys Leu Ile Thr Gln Arg Lys 
                885                 890                 895     


Phe Asp Asn Leu Thr Lys Ala Glu Arg Gly Gly Leu Ser Glu Leu Asp 
            900                 905                 910         


Lys Ala Gly Phe Ile Lys Arg Gln Leu Val Glu Thr Arg Gln Ile Thr 
        915                 920                 925             


Lys His Val Ala Gln Ile Leu Asp Ser Arg Met Asn Thr Lys Tyr Asp 
    930                 935                 940                 


Glu Asn Asp Lys Leu Ile Arg Glu Val Lys Val Ile Thr Leu Lys Ser 
945                 950                 955                 960 


Lys Leu Val Ser Asp Phe Arg Lys Asp Phe Gln Phe Tyr Lys Val Arg 
                965                 970                 975     


Glu Ile Asn Asn Tyr His His Ala His Asp Ala Tyr Leu Asn Ala Val 
            980                 985                 990         


Val Gly Thr Ala Leu Ile Lys Lys  Tyr Pro Lys Leu Glu  Ser Glu Phe 
        995                 1000                 1005             


Val Tyr  Gly Asp Tyr Lys Val  Tyr Asp Val Arg Lys  Met Ile Ala 
    1010                 1015                 1020             


Lys Ser  Glu Gln Glu Ile Gly  Lys Ala Thr Ala Lys  Tyr Phe Phe 
    1025                 1030                 1035             


Tyr Ser  Asn Ile Met Asn Phe  Phe Lys Thr Glu Ile  Thr Leu Ala 
    1040                 1045                 1050             


Asn Gly  Glu Ile Arg Lys Arg  Pro Leu Ile Glu Thr  Asn Gly Glu 
    1055                 1060                 1065             


Thr Gly  Glu Ile Val Trp Asp  Lys Gly Arg Asp Phe  Ala Thr Val 
    1070                 1075                 1080             


Arg Lys  Val Leu Ser Met Pro  Gln Val Asn Ile Val  Lys Lys Thr 
    1085                 1090                 1095             


Glu Val  Gln Thr Gly Gly Phe  Ser Lys Glu Ser Ile  Leu Pro Lys 
    1100                 1105                 1110             


Arg Asn  Ser Asp Lys Leu Ile  Ala Arg Lys Lys Asp  Trp Asp Pro 
    1115                 1120                 1125             


Lys Lys  Tyr Gly Gly Phe Asp  Ser Pro Thr Val Ala  Tyr Ser Val 
    1130                 1135                 1140             


Leu Val  Val Ala Lys Val Glu  Lys Gly Lys Ser Lys  Lys Leu Lys 
    1145                 1150                 1155             


Ser Val  Lys Glu Leu Leu Gly  Ile Thr Ile Met Glu  Arg Ser Ser 
    1160                 1165                 1170             


Phe Glu  Lys Asn Pro Ile Asp  Phe Leu Glu Ala Lys  Gly Tyr Lys 
    1175                 1180                 1185             


Glu Val  Lys Lys Asp Leu Ile  Ile Lys Leu Pro Lys  Tyr Ser Leu 
    1190                 1195                 1200             


Phe Glu  Leu Glu Asn Gly Arg  Lys Arg Met Leu Ala  Ser Ala Gly 
    1205                 1210                 1215             


Glu Leu  Gln Lys Gly Asn Glu  Leu Ala Leu Pro Ser  Lys Tyr Val 
    1220                 1225                 1230             


Asn Phe  Leu Tyr Leu Ala Ser  His Tyr Glu Lys Leu  Lys Gly Ser 
    1235                 1240                 1245             


Pro Glu  Asp Asn Glu Gln Lys  Gln Leu Phe Val Glu  Gln His Lys 
    1250                 1255                 1260             


His Tyr  Leu Asp Glu Ile Ile  Glu Gln Ile Ser Glu  Phe Ser Lys 
    1265                 1270                 1275             


Arg Val  Ile Leu Ala Asp Ala  Asn Leu Asp Lys Val  Leu Ser Ala 
    1280                 1285                 1290             


Tyr Asn  Lys His Arg Asp Lys  Pro Ile Arg Glu Gln  Ala Glu Asn 
    1295                 1300                 1305             


Ile Ile  His Leu Phe Thr Leu  Thr Asn Leu Gly Ala  Pro Ala Ala 
    1310                 1315                 1320             


Phe Lys  Tyr Phe Asp Thr Thr  Ile Asp Arg Lys Arg  Tyr Thr Ser 
    1325                 1330                 1335             


Thr Lys  Glu Val Leu Asp Ala  Thr Leu Ile His Gln  Ser Ile Thr 
    1340                 1345                 1350             


Gly Leu  Tyr Glu Thr Arg Ile  Asp Leu Ser Gln Leu  Gly Gly Asp 
    1355                 1360                 1365             


Glu Gly  Ala Asp Pro Lys Lys  Lys Arg Lys Val Asp  Pro Lys Lys 
    1370                 1375                 1380             


Lys Arg  Lys Val Asp Pro Lys  Lys Lys Arg Lys Val  Gly Ser Leu 
    1385                 1390                 1395             


Pro Thr  Cys Ser Cys Leu Asp  Arg Val Ile Gln Lys  Asp Lys Gly 
    1400                 1405                 1410             


Pro Tyr  Tyr Thr His Leu Gly  Ala Gly Pro Ser Val  Ala Ala Val 
    1415                 1420                 1425             


Arg Glu  Ile Met Glu Asn Arg  Tyr Gly Gln Lys Gly  Asn Ala Ile 
    1430                 1435                 1440             


Arg Ile  Glu Ile Val Val Tyr  Thr Gly Lys Glu Gly  Lys Ser Ser 
    1445                 1450                 1455             


His Gly  Cys Pro Ile Ala Lys  Trp Val Leu Arg Arg  Ser Ser Asp 
    1460                 1465                 1470             


Glu Glu  Lys Val Leu Cys Leu  Val Arg Gln Arg Thr  Gly His His 
    1475                 1480                 1485             


Cys Pro  Thr Ala Val Met Val  Val Leu Ile Met Val  Trp Asp Gly 
    1490                 1495                 1500             


Ile Pro  Leu Pro Met Ala Asp  Arg Leu Tyr Thr Glu  Leu Thr Glu 
    1505                 1510                 1515             


Asn Leu  Lys Ser Tyr Asn Gly  His Pro Thr Asp Arg  Arg Cys Thr 
    1520                 1525                 1530             


Leu Asn  Glu Asn Arg Thr Cys  Thr Cys Gln Gly Ile  Asp Pro Glu 
    1535                 1540                 1545             


Thr Cys  Gly Ala Ser Phe Ser  Phe Gly Cys Ser Trp  Ser Met Tyr 
    1550                 1555                 1560             


Phe Asn  Gly Cys Lys Phe Gly  Arg Ser Pro Ser Pro  Arg Arg Phe 
    1565                 1570                 1575             


Arg Ile  Asp Pro Ser Ser Pro  Leu His Glu Lys Asn  Leu Glu Asp 
    1580                 1585                 1590             


Asn Leu  Gln Ser Leu Ala Thr  Arg Leu Ala Pro Ile  Tyr Lys Gln 
    1595                 1600                 1605             


Tyr Ala  Pro Val Ala Tyr Gln  Asn Gln Val Glu Tyr  Glu Asn Val 
    1610                 1615                 1620             


Ala Arg  Glu Cys Arg Leu Gly  Ser Lys Glu Gly Arg  Pro Phe Ser 
    1625                 1630                 1635             


Gly Val  Thr Ala Cys Leu Asp  Phe Cys Ala His Pro  His Arg Asp 
    1640                 1645                 1650             


Ile His  Asn Met Asn Asn Gly  Ser Thr Val Val Cys  Thr Leu Thr 
    1655                 1660                 1665             


Arg Glu  Asp Asn Arg Ser Leu  Gly Val Ile Pro Gln  Asp Glu Gln 
    1670                 1675                 1680             


Leu His  Val Leu Pro Leu Tyr  Lys Leu Ser Asp Thr  Asp Glu Phe 
    1685                 1690                 1695             


Gly Ser  Lys Glu Gly Met Glu  Ala Lys Ile Lys Ser  Gly Ala Ile 
    1700                 1705                 1710             


Glu Val  Leu Ala Pro Arg Arg  Lys Lys Arg Thr Cys  Phe Thr Gln 
    1715                 1720                 1725             


Pro Val  Pro Arg Ser Gly Lys  Lys Arg Ala Ala Met  Met Thr Glu 
    1730                 1735                 1740             


Val Leu  Ala His Lys Ile Arg  Ala Val Glu Lys Lys  Pro Ile Pro 
    1745                 1750                 1755             


Arg Ile  Lys Arg Lys Asn Asn  Ser Thr Thr Thr Asn  Asn Ser Lys 
    1760                 1765                 1770             


Pro Ser  Ser Leu Pro Thr Leu  Gly Ser Asn Thr Glu  Thr Val Gln 
    1775                 1780                 1785             


Pro Glu  Val Lys Ser Glu Thr  Glu Pro His Phe Ile  Leu Lys Ser 
    1790                 1795                 1800             


Ser Asp  Asn Thr Lys Thr Tyr  Ser Leu Met Pro Ser  Ala Pro His 
    1805                 1810                 1815             


Pro Val  Lys Glu Ala Ser Pro  Gly Phe Ser Trp Ser  Pro Lys Thr 
    1820                 1825                 1830             


Ala Ser  Ala Thr Pro Ala Pro  Leu Lys Asn Asp Ala  Thr Ala Ser 
    1835                 1840                 1845             


Cys Gly  Phe Ser Glu Arg Ser  Ser Thr Pro His Cys  Thr Met Pro 
    1850                 1855                 1860             


Ser Gly  Arg Leu Ser Gly Ala  Asn Ala Ala Ala Ala  Asp Gly Pro 
    1865                 1870                 1875             


Gly Ile  Ser Gln Leu Gly Glu  Val Ala Pro Leu Pro  Thr Leu Ser 
    1880                 1885                 1890             


Ala Pro  Val Met Glu Pro Leu  Ile Asn Ser Glu Pro  Ser Thr Gly 
    1895                 1900                 1905             


Val Thr  Glu Pro Leu Thr Pro  His Gln Pro Asn His  Gln Pro Ser 
    1910                 1915                 1920             


Phe Leu  Thr Ser Pro Gln Asp  Leu Ala Ser Ser Pro  Met Glu Glu 
    1925                 1930                 1935             


Asp Glu  Gln His Ser Glu Ala  Asp Glu Pro Pro Ser  Asp Glu Pro 
    1940                 1945                 1950             


Leu Ser  Asp Asp Pro Leu Ser  Pro Ala Glu Glu Lys  Leu Pro His 
    1955                 1960                 1965             


Ile Asp  Glu Tyr Trp Ser Asp  Ser Glu His Ile Phe  Leu Asp Ala 
    1970                 1975                 1980             


Asn Ile  Gly Gly Val Ala Ile  Ala Pro Ala His Gly  Ser Val Leu 
    1985                 1990                 1995             


Ile Glu  Cys Ala Arg Arg Glu  Leu His Ala Thr Thr  Pro Val Glu 
    2000                 2005                 2010             


His Pro  Asn Arg Asn His Pro  Thr Arg Leu Ser Leu  Val Phe Tyr 
    2015                 2020                 2025             


Gln His  Lys Asn Leu Asn Lys  Pro Gln His Gly Phe  Glu Leu Asn 
    2030                 2035                 2040             


Lys Ile  Lys Phe Glu Ala Lys  Glu Ala Lys Asn Lys  Lys Met Lys 
    2045                 2050                 2055             


Ala Ser  Glu Gln Lys Asp Gln  Ala Ala Asn Glu Gly  Pro Glu Gln 
    2060                 2065                 2070             


Ser Ser  Glu Val Asn Glu Leu  Asn Gln Ile Pro Ser  His Lys Ala 
    2075                 2080                 2085             


Leu Thr  Leu Thr His Asp Asn  Val Val Thr Val Ser  Pro Tyr Ala 
    2090                 2095                 2100             


Leu Thr  His Val Ala Gly Pro  Tyr Asn His Trp Val  
    2105                 2110                 2115 


<210>  160
<211>  2115
<212>  PRT
<213>  Unknown

<220>
<223>  dCAS9-Tet1CD_IM

<400>  160

Met Asp Lys Lys Tyr Ser Ile Gly Leu Ala Ile Gly Thr Asn Ser Val 
1               5                   10                  15      


Gly Trp Ala Val Ile Thr Asp Glu Tyr Lys Val Pro Ser Lys Lys Phe 
            20                  25                  30          


Lys Val Leu Gly Asn Thr Asp Arg His Ser Ile Lys Lys Asn Leu Ile 
        35                  40                  45              


Gly Ala Leu Leu Phe Asp Ser Gly Glu Thr Ala Glu Ala Thr Arg Leu 
    50                  55                  60                  


Lys Arg Thr Ala Arg Arg Arg Tyr Thr Arg Arg Lys Asn Arg Ile Cys 
65                  70                  75                  80  


Tyr Leu Gln Glu Ile Phe Ser Asn Glu Met Ala Lys Val Asp Asp Ser 
                85                  90                  95      


Phe Phe His Arg Leu Glu Glu Ser Phe Leu Val Glu Glu Asp Lys Lys 
            100                 105                 110         


His Glu Arg His Pro Ile Phe Gly Asn Ile Val Asp Glu Val Ala Tyr 
        115                 120                 125             


His Glu Lys Tyr Pro Thr Ile Tyr His Leu Arg Lys Lys Leu Val Asp 
    130                 135                 140                 


Ser Thr Asp Lys Ala Asp Leu Arg Leu Ile Tyr Leu Ala Leu Ala His 
145                 150                 155                 160 


Met Ile Lys Phe Arg Gly His Phe Leu Ile Glu Gly Asp Leu Asn Pro 
                165                 170                 175     


Asp Asn Ser Asp Val Asp Lys Leu Phe Ile Gln Leu Val Gln Thr Tyr 
            180                 185                 190         


Asn Gln Leu Phe Glu Glu Asn Pro Ile Asn Ala Ser Gly Val Asp Ala 
        195                 200                 205             


Lys Ala Ile Leu Ser Ala Arg Leu Ser Lys Ser Arg Arg Leu Glu Asn 
    210                 215                 220                 


Leu Ile Ala Gln Leu Pro Gly Glu Lys Lys Asn Gly Leu Phe Gly Asn 
225                 230                 235                 240 


Leu Ile Ala Leu Ser Leu Gly Leu Thr Pro Asn Phe Lys Ser Asn Phe 
                245                 250                 255     


Asp Leu Ala Glu Asp Ala Lys Leu Gln Leu Ser Lys Asp Thr Tyr Asp 
            260                 265                 270         


Asp Asp Leu Asp Asn Leu Leu Ala Gln Ile Gly Asp Gln Tyr Ala Asp 
        275                 280                 285             


Leu Phe Leu Ala Ala Lys Asn Leu Ser Asp Ala Ile Leu Leu Ser Asp 
    290                 295                 300                 


Ile Leu Arg Val Asn Thr Glu Ile Thr Lys Ala Pro Leu Ser Ala Ser 
305                 310                 315                 320 


Met Ile Lys Arg Tyr Asp Glu His His Gln Asp Leu Thr Leu Leu Lys 
                325                 330                 335     


Ala Leu Val Arg Gln Gln Leu Pro Glu Lys Tyr Lys Glu Ile Phe Phe 
            340                 345                 350         


Asp Gln Ser Lys Asn Gly Tyr Ala Gly Tyr Ile Asp Gly Gly Ala Ser 
        355                 360                 365             


Gln Glu Glu Phe Tyr Lys Phe Ile Lys Pro Ile Leu Glu Lys Met Asp 
    370                 375                 380                 


Gly Thr Glu Glu Leu Leu Val Lys Leu Asn Arg Glu Asp Leu Leu Arg 
385                 390                 395                 400 


Lys Gln Arg Thr Phe Asp Asn Gly Ser Ile Pro His Gln Ile His Leu 
                405                 410                 415     


Gly Glu Leu His Ala Ile Leu Arg Arg Gln Glu Asp Phe Tyr Pro Phe 
            420                 425                 430         


Leu Lys Asp Asn Arg Glu Lys Ile Glu Lys Ile Leu Thr Phe Arg Ile 
        435                 440                 445             


Pro Tyr Tyr Val Gly Pro Leu Ala Arg Gly Asn Ser Arg Phe Ala Trp 
    450                 455                 460                 


Met Thr Arg Lys Ser Glu Glu Thr Ile Thr Pro Trp Asn Phe Glu Glu 
465                 470                 475                 480 


Val Val Asp Lys Gly Ala Ser Ala Gln Ser Phe Ile Glu Arg Met Thr 
                485                 490                 495     


Asn Phe Asp Lys Asn Leu Pro Asn Glu Lys Val Leu Pro Lys His Ser 
            500                 505                 510         


Leu Leu Tyr Glu Tyr Phe Thr Val Tyr Asn Glu Leu Thr Lys Val Lys 
        515                 520                 525             


Tyr Val Thr Glu Gly Met Arg Lys Pro Ala Phe Leu Ser Gly Glu Gln 
    530                 535                 540                 


Lys Lys Ala Ile Val Asp Leu Leu Phe Lys Thr Asn Arg Lys Val Thr 
545                 550                 555                 560 


Val Lys Gln Leu Lys Glu Asp Tyr Phe Lys Lys Ile Glu Cys Phe Asp 
                565                 570                 575     


Ser Val Glu Ile Ser Gly Val Glu Asp Arg Phe Asn Ala Ser Leu Gly 
            580                 585                 590         


Thr Tyr His Asp Leu Leu Lys Ile Ile Lys Asp Lys Asp Phe Leu Asp 
        595                 600                 605             


Asn Glu Glu Asn Glu Asp Ile Leu Glu Asp Ile Val Leu Thr Leu Thr 
    610                 615                 620                 


Leu Phe Glu Asp Arg Glu Met Ile Glu Glu Arg Leu Lys Thr Tyr Ala 
625                 630                 635                 640 


His Leu Phe Asp Asp Lys Val Met Lys Gln Leu Lys Arg Arg Arg Tyr 
                645                 650                 655     


Thr Gly Trp Gly Arg Leu Ser Arg Lys Leu Ile Asn Gly Ile Arg Asp 
            660                 665                 670         


Lys Gln Ser Gly Lys Thr Ile Leu Asp Phe Leu Lys Ser Asp Gly Phe 
        675                 680                 685             


Ala Asn Arg Asn Phe Met Gln Leu Ile His Asp Asp Ser Leu Thr Phe 
    690                 695                 700                 


Lys Glu Asp Ile Gln Lys Ala Gln Val Ser Gly Gln Gly Asp Ser Leu 
705                 710                 715                 720 


His Glu His Ile Ala Asn Leu Ala Gly Ser Pro Ala Ile Lys Lys Gly 
                725                 730                 735     


Ile Leu Gln Thr Val Lys Val Val Asp Glu Leu Val Lys Val Met Gly 
            740                 745                 750         


Arg His Lys Pro Glu Asn Ile Val Ile Glu Met Ala Arg Glu Asn Gln 
        755                 760                 765             


Thr Thr Gln Lys Gly Gln Lys Asn Ser Arg Glu Arg Met Lys Arg Ile 
    770                 775                 780                 


Glu Glu Gly Ile Lys Glu Leu Gly Ser Gln Ile Leu Lys Glu His Pro 
785                 790                 795                 800 


Val Glu Asn Thr Gln Leu Gln Asn Glu Lys Leu Tyr Leu Tyr Tyr Leu 
                805                 810                 815     


Gln Asn Gly Arg Asp Met Tyr Val Asp Gln Glu Leu Asp Ile Asn Arg 
            820                 825                 830         


Leu Ser Asp Tyr Asp Val Asp Ala Ile Val Pro Gln Ser Phe Leu Lys 
        835                 840                 845             


Asp Asp Ser Ile Asp Asn Lys Val Leu Thr Arg Ser Asp Lys Asn Arg 
    850                 855                 860                 


Gly Lys Ser Asp Asn Val Pro Ser Glu Glu Val Val Lys Lys Met Lys 
865                 870                 875                 880 


Asn Tyr Trp Arg Gln Leu Leu Asn Ala Lys Leu Ile Thr Gln Arg Lys 
                885                 890                 895     


Phe Asp Asn Leu Thr Lys Ala Glu Arg Gly Gly Leu Ser Glu Leu Asp 
            900                 905                 910         


Lys Ala Gly Phe Ile Lys Arg Gln Leu Val Glu Thr Arg Gln Ile Thr 
        915                 920                 925             


Lys His Val Ala Gln Ile Leu Asp Ser Arg Met Asn Thr Lys Tyr Asp 
    930                 935                 940                 


Glu Asn Asp Lys Leu Ile Arg Glu Val Lys Val Ile Thr Leu Lys Ser 
945                 950                 955                 960 


Lys Leu Val Ser Asp Phe Arg Lys Asp Phe Gln Phe Tyr Lys Val Arg 
                965                 970                 975     


Glu Ile Asn Asn Tyr His His Ala His Asp Ala Tyr Leu Asn Ala Val 
            980                 985                 990         


Val Gly Thr Ala Leu Ile Lys Lys  Tyr Pro Lys Leu Glu  Ser Glu Phe 
        995                 1000                 1005             


Val Tyr  Gly Asp Tyr Lys Val  Tyr Asp Val Arg Lys  Met Ile Ala 
    1010                 1015                 1020             


Lys Ser  Glu Gln Glu Ile Gly  Lys Ala Thr Ala Lys  Tyr Phe Phe 
    1025                 1030                 1035             


Tyr Ser  Asn Ile Met Asn Phe  Phe Lys Thr Glu Ile  Thr Leu Ala 
    1040                 1045                 1050             


Asn Gly  Glu Ile Arg Lys Arg  Pro Leu Ile Glu Thr  Asn Gly Glu 
    1055                 1060                 1065             


Thr Gly  Glu Ile Val Trp Asp  Lys Gly Arg Asp Phe  Ala Thr Val 
    1070                 1075                 1080             


Arg Lys  Val Leu Ser Met Pro  Gln Val Asn Ile Val  Lys Lys Thr 
    1085                 1090                 1095             


Glu Val  Gln Thr Gly Gly Phe  Ser Lys Glu Ser Ile  Leu Pro Lys 
    1100                 1105                 1110             


Arg Asn  Ser Asp Lys Leu Ile  Ala Arg Lys Lys Asp  Trp Asp Pro 
    1115                 1120                 1125             


Lys Lys  Tyr Gly Gly Phe Asp  Ser Pro Thr Val Ala  Tyr Ser Val 
    1130                 1135                 1140             


Leu Val  Val Ala Lys Val Glu  Lys Gly Lys Ser Lys  Lys Leu Lys 
    1145                 1150                 1155             


Ser Val  Lys Glu Leu Leu Gly  Ile Thr Ile Met Glu  Arg Ser Ser 
    1160                 1165                 1170             


Phe Glu  Lys Asn Pro Ile Asp  Phe Leu Glu Ala Lys  Gly Tyr Lys 
    1175                 1180                 1185             


Glu Val  Lys Lys Asp Leu Ile  Ile Lys Leu Pro Lys  Tyr Ser Leu 
    1190                 1195                 1200             


Phe Glu  Leu Glu Asn Gly Arg  Lys Arg Met Leu Ala  Ser Ala Gly 
    1205                 1210                 1215             


Glu Leu  Gln Lys Gly Asn Glu  Leu Ala Leu Pro Ser  Lys Tyr Val 
    1220                 1225                 1230             


Asn Phe  Leu Tyr Leu Ala Ser  His Tyr Glu Lys Leu  Lys Gly Ser 
    1235                 1240                 1245             


Pro Glu  Asp Asn Glu Gln Lys  Gln Leu Phe Val Glu  Gln His Lys 
    1250                 1255                 1260             


His Tyr  Leu Asp Glu Ile Ile  Glu Gln Ile Ser Glu  Phe Ser Lys 
    1265                 1270                 1275             


Arg Val  Ile Leu Ala Asp Ala  Asn Leu Asp Lys Val  Leu Ser Ala 
    1280                 1285                 1290             


Tyr Asn  Lys His Arg Asp Lys  Pro Ile Arg Glu Gln  Ala Glu Asn 
    1295                 1300                 1305             


Ile Ile  His Leu Phe Thr Leu  Thr Asn Leu Gly Ala  Pro Ala Ala 
    1310                 1315                 1320             


Phe Lys  Tyr Phe Asp Thr Thr  Ile Asp Arg Lys Arg  Tyr Thr Ser 
    1325                 1330                 1335             


Thr Lys  Glu Val Leu Asp Ala  Thr Leu Ile His Gln  Ser Ile Thr 
    1340                 1345                 1350             


Gly Leu  Tyr Glu Thr Arg Ile  Asp Leu Ser Gln Leu  Gly Gly Asp 
    1355                 1360                 1365             


Glu Gly  Ala Asp Pro Lys Lys  Lys Arg Lys Val Asp  Pro Lys Lys 
    1370                 1375                 1380             


Lys Arg  Lys Val Asp Pro Lys  Lys Lys Arg Lys Val  Gly Ser Leu 
    1385                 1390                 1395             


Pro Thr  Cys Ser Cys Leu Asp  Arg Val Ile Gln Lys  Asp Lys Gly 
    1400                 1405                 1410             


Pro Tyr  Tyr Thr His Leu Gly  Ala Gly Pro Ser Val  Ala Ala Val 
    1415                 1420                 1425             


Arg Glu  Ile Met Glu Asn Arg  Tyr Gly Gln Lys Gly  Asn Ala Ile 
    1430                 1435                 1440             


Arg Ile  Glu Ile Val Val Tyr  Thr Gly Lys Glu Gly  Lys Ser Ser 
    1445                 1450                 1455             


His Gly  Cys Pro Ile Ala Lys  Trp Val Leu Arg Arg  Ser Ser Asp 
    1460                 1465                 1470             


Glu Glu  Lys Val Leu Cys Leu  Val Arg Gln Arg Thr  Gly His His 
    1475                 1480                 1485             


Cys Pro  Thr Ala Val Met Val  Val Leu Ile Met Val  Trp Asp Gly 
    1490                 1495                 1500             


Ile Pro  Leu Pro Met Ala Asp  Arg Leu Tyr Thr Glu  Leu Thr Glu 
    1505                 1510                 1515             


Asn Leu  Lys Ser Tyr Asn Gly  His Pro Thr Asp Arg  Arg Cys Thr 
    1520                 1525                 1530             


Leu Asn  Glu Asn Arg Thr Cys  Thr Cys Gln Gly Ile  Asp Pro Glu 
    1535                 1540                 1545             


Thr Cys  Gly Ala Ser Phe Ser  Phe Gly Cys Ser Trp  Ser Met Tyr 
    1550                 1555                 1560             


Phe Asn  Gly Cys Lys Phe Gly  Arg Ser Pro Ser Pro  Arg Arg Phe 
    1565                 1570                 1575             


Arg Ile  Asp Pro Ser Ser Pro  Leu His Glu Lys Asn  Leu Glu Asp 
    1580                 1585                 1590             


Asn Leu  Gln Ser Leu Ala Thr  Arg Leu Ala Pro Ile  Tyr Lys Gln 
    1595                 1600                 1605             


Tyr Ala  Pro Val Ala Tyr Gln  Asn Gln Val Glu Tyr  Glu Asn Val 
    1610                 1615                 1620             


Ala Arg  Glu Cys Arg Leu Gly  Ser Lys Glu Gly Arg  Pro Phe Ser 
    1625                 1630                 1635             


Gly Val  Thr Ala Cys Leu Asp  Phe Cys Ala His Pro  Tyr Arg Ala 
    1640                 1645                 1650             


Ile His  Asn Met Asn Asn Gly  Ser Thr Val Val Cys  Thr Leu Thr 
    1655                 1660                 1665             


Arg Glu  Asp Asn Arg Ser Leu  Gly Val Ile Pro Gln  Asp Glu Gln 
    1670                 1675                 1680             


Leu His  Val Leu Pro Leu Tyr  Lys Leu Ser Asp Thr  Asp Glu Phe 
    1685                 1690                 1695             


Gly Ser  Lys Glu Gly Met Glu  Ala Lys Ile Lys Ser  Gly Ala Ile 
    1700                 1705                 1710             


Glu Val  Leu Ala Pro Arg Arg  Lys Lys Arg Thr Cys  Phe Thr Gln 
    1715                 1720                 1725             


Pro Val  Pro Arg Ser Gly Lys  Lys Arg Ala Ala Met  Met Thr Glu 
    1730                 1735                 1740             


Val Leu  Ala His Lys Ile Arg  Ala Val Glu Lys Lys  Pro Ile Pro 
    1745                 1750                 1755             


Arg Ile  Lys Arg Lys Asn Asn  Ser Thr Thr Thr Asn  Asn Ser Lys 
    1760                 1765                 1770             


Pro Ser  Ser Leu Pro Thr Leu  Gly Ser Asn Thr Glu  Thr Val Gln 
    1775                 1780                 1785             


Pro Glu  Val Lys Ser Glu Thr  Glu Pro His Phe Ile  Leu Lys Ser 
    1790                 1795                 1800             


Ser Asp  Asn Thr Lys Thr Tyr  Ser Leu Met Pro Ser  Ala Pro His 
    1805                 1810                 1815             


Pro Val  Lys Glu Ala Ser Pro  Gly Phe Ser Trp Ser  Pro Lys Thr 
    1820                 1825                 1830             


Ala Ser  Ala Thr Pro Ala Pro  Leu Lys Asn Asp Ala  Thr Ala Ser 
    1835                 1840                 1845             


Cys Gly  Phe Ser Glu Arg Ser  Ser Thr Pro His Cys  Thr Met Pro 
    1850                 1855                 1860             


Ser Gly  Arg Leu Ser Gly Ala  Asn Ala Ala Ala Ala  Asp Gly Pro 
    1865                 1870                 1875             


Gly Ile  Ser Gln Leu Gly Glu  Val Ala Pro Leu Pro  Thr Leu Ser 
    1880                 1885                 1890             


Ala Pro  Val Met Glu Pro Leu  Ile Asn Ser Glu Pro  Ser Thr Gly 
    1895                 1900                 1905             


Val Thr  Glu Pro Leu Thr Pro  His Gln Pro Asn His  Gln Pro Ser 
    1910                 1915                 1920             


Phe Leu  Thr Ser Pro Gln Asp  Leu Ala Ser Ser Pro  Met Glu Glu 
    1925                 1930                 1935             


Asp Glu  Gln His Ser Glu Ala  Asp Glu Pro Pro Ser  Asp Glu Pro 
    1940                 1945                 1950             


Leu Ser  Asp Asp Pro Leu Ser  Pro Ala Glu Glu Lys  Leu Pro His 
    1955                 1960                 1965             


Ile Asp  Glu Tyr Trp Ser Asp  Ser Glu His Ile Phe  Leu Asp Ala 
    1970                 1975                 1980             


Asn Ile  Gly Gly Val Ala Ile  Ala Pro Ala His Gly  Ser Val Leu 
    1985                 1990                 1995             


Ile Glu  Cys Ala Arg Arg Glu  Leu His Ala Thr Thr  Pro Val Glu 
    2000                 2005                 2010             


His Pro  Asn Arg Asn His Pro  Thr Arg Leu Ser Leu  Val Phe Tyr 
    2015                 2020                 2025             


Gln His  Lys Asn Leu Asn Lys  Pro Gln His Gly Phe  Glu Leu Asn 
    2030                 2035                 2040             


Lys Ile  Lys Phe Glu Ala Lys  Glu Ala Lys Asn Lys  Lys Met Lys 
    2045                 2050                 2055             


Ala Ser  Glu Gln Lys Asp Gln  Ala Ala Asn Glu Gly  Pro Glu Gln 
    2060                 2065                 2070             


Ser Ser  Glu Val Asn Glu Leu  Asn Gln Ile Pro Ser  His Lys Ala 
    2075                 2080                 2085             


Leu Thr  Leu Thr His Asp Asn  Val Val Thr Val Ser  Pro Tyr Ala 
    2090                 2095                 2100             


Leu Thr  His Val Ala Gly Pro  Tyr Asn His Trp Val  
    2105                 2110                 2115 


<210>  161
<211>  2573
<212>  PRT
<213>  Unknown

<220>
<223>  dCAS-Dnmt3a-P2A-BFP

<400>  161

Met Asp Lys Lys Tyr Ser Ile Gly Leu Ala Ile Gly Thr Asn Ser Val 
1               5                   10                  15      


Gly Trp Ala Val Ile Thr Asp Glu Tyr Lys Val Pro Ser Lys Lys Phe 
            20                  25                  30          


Lys Val Leu Gly Asn Thr Asp Arg His Ser Ile Lys Lys Asn Leu Ile 
        35                  40                  45              


Gly Ala Leu Leu Phe Asp Ser Gly Glu Thr Ala Glu Ala Thr Arg Leu 
    50                  55                  60                  


Lys Arg Thr Ala Arg Arg Arg Tyr Thr Arg Arg Lys Asn Arg Ile Cys 
65                  70                  75                  80  


Tyr Leu Gln Glu Ile Phe Ser Asn Glu Met Ala Lys Val Asp Asp Ser 
                85                  90                  95      


Phe Phe His Arg Leu Glu Glu Ser Phe Leu Val Glu Glu Asp Lys Lys 
            100                 105                 110         


His Glu Arg His Pro Ile Phe Gly Asn Ile Val Asp Glu Val Ala Tyr 
        115                 120                 125             


His Glu Lys Tyr Pro Thr Ile Tyr His Leu Arg Lys Lys Leu Val Asp 
    130                 135                 140                 


Ser Thr Asp Lys Ala Asp Leu Arg Leu Ile Tyr Leu Ala Leu Ala His 
145                 150                 155                 160 


Met Ile Lys Phe Arg Gly His Phe Leu Ile Glu Gly Asp Leu Asn Pro 
                165                 170                 175     


Asp Asn Ser Asp Val Asp Lys Leu Phe Ile Gln Leu Val Gln Thr Tyr 
            180                 185                 190         


Asn Gln Leu Phe Glu Glu Asn Pro Ile Asn Ala Ser Gly Val Asp Ala 
        195                 200                 205             


Lys Ala Ile Leu Ser Ala Arg Leu Ser Lys Ser Arg Arg Leu Glu Asn 
    210                 215                 220                 


Leu Ile Ala Gln Leu Pro Gly Glu Lys Lys Asn Gly Leu Phe Gly Asn 
225                 230                 235                 240 


Leu Ile Ala Leu Ser Leu Gly Leu Thr Pro Asn Phe Lys Ser Asn Phe 
                245                 250                 255     


Asp Leu Ala Glu Asp Ala Lys Leu Gln Leu Ser Lys Asp Thr Tyr Asp 
            260                 265                 270         


Asp Asp Leu Asp Asn Leu Leu Ala Gln Ile Gly Asp Gln Tyr Ala Asp 
        275                 280                 285             


Leu Phe Leu Ala Ala Lys Asn Leu Ser Asp Ala Ile Leu Leu Ser Asp 
    290                 295                 300                 


Ile Leu Arg Val Asn Thr Glu Ile Thr Lys Ala Pro Leu Ser Ala Ser 
305                 310                 315                 320 


Met Ile Lys Arg Tyr Asp Glu His His Gln Asp Leu Thr Leu Leu Lys 
                325                 330                 335     


Ala Leu Val Arg Gln Gln Leu Pro Glu Lys Tyr Lys Glu Ile Phe Phe 
            340                 345                 350         


Asp Gln Ser Lys Asn Gly Tyr Ala Gly Tyr Ile Asp Gly Gly Ala Ser 
        355                 360                 365             


Gln Glu Glu Phe Tyr Lys Phe Ile Lys Pro Ile Leu Glu Lys Met Asp 
    370                 375                 380                 


Gly Thr Glu Glu Leu Leu Val Lys Leu Asn Arg Glu Asp Leu Leu Arg 
385                 390                 395                 400 


Lys Gln Arg Thr Phe Asp Asn Gly Ser Ile Pro His Gln Ile His Leu 
                405                 410                 415     


Gly Glu Leu His Ala Ile Leu Arg Arg Gln Glu Asp Phe Tyr Pro Phe 
            420                 425                 430         


Leu Lys Asp Asn Arg Glu Lys Ile Glu Lys Ile Leu Thr Phe Arg Ile 
        435                 440                 445             


Pro Tyr Tyr Val Gly Pro Leu Ala Arg Gly Asn Ser Arg Phe Ala Trp 
    450                 455                 460                 


Met Thr Arg Lys Ser Glu Glu Thr Ile Thr Pro Trp Asn Phe Glu Glu 
465                 470                 475                 480 


Val Val Asp Lys Gly Ala Ser Ala Gln Ser Phe Ile Glu Arg Met Thr 
                485                 490                 495     


Asn Phe Asp Lys Asn Leu Pro Asn Glu Lys Val Leu Pro Lys His Ser 
            500                 505                 510         


Leu Leu Tyr Glu Tyr Phe Thr Val Tyr Asn Glu Leu Thr Lys Val Lys 
        515                 520                 525             


Tyr Val Thr Glu Gly Met Arg Lys Pro Ala Phe Leu Ser Gly Glu Gln 
    530                 535                 540                 


Lys Lys Ala Ile Val Asp Leu Leu Phe Lys Thr Asn Arg Lys Val Thr 
545                 550                 555                 560 


Val Lys Gln Leu Lys Glu Asp Tyr Phe Lys Lys Ile Glu Cys Phe Asp 
                565                 570                 575     


Ser Val Glu Ile Ser Gly Val Glu Asp Arg Phe Asn Ala Ser Leu Gly 
            580                 585                 590         


Thr Tyr His Asp Leu Leu Lys Ile Ile Lys Asp Lys Asp Phe Leu Asp 
        595                 600                 605             


Asn Glu Glu Asn Glu Asp Ile Leu Glu Asp Ile Val Leu Thr Leu Thr 
    610                 615                 620                 


Leu Phe Glu Asp Arg Glu Met Ile Glu Glu Arg Leu Lys Thr Tyr Ala 
625                 630                 635                 640 


His Leu Phe Asp Asp Lys Val Met Lys Gln Leu Lys Arg Arg Arg Tyr 
                645                 650                 655     


Thr Gly Trp Gly Arg Leu Ser Arg Lys Leu Ile Asn Gly Ile Arg Asp 
            660                 665                 670         


Lys Gln Ser Gly Lys Thr Ile Leu Asp Phe Leu Lys Ser Asp Gly Phe 
        675                 680                 685             


Ala Asn Arg Asn Phe Met Gln Leu Ile His Asp Asp Ser Leu Thr Phe 
    690                 695                 700                 


Lys Glu Asp Ile Gln Lys Ala Gln Val Ser Gly Gln Gly Asp Ser Leu 
705                 710                 715                 720 


His Glu His Ile Ala Asn Leu Ala Gly Ser Pro Ala Ile Lys Lys Gly 
                725                 730                 735     


Ile Leu Gln Thr Val Lys Val Val Asp Glu Leu Val Lys Val Met Gly 
            740                 745                 750         


Arg His Lys Pro Glu Asn Ile Val Ile Glu Met Ala Arg Glu Asn Gln 
        755                 760                 765             


Thr Thr Gln Lys Gly Gln Lys Asn Ser Arg Glu Arg Met Lys Arg Ile 
    770                 775                 780                 


Glu Glu Gly Ile Lys Glu Leu Gly Ser Gln Ile Leu Lys Glu His Pro 
785                 790                 795                 800 


Val Glu Asn Thr Gln Leu Gln Asn Glu Lys Leu Tyr Leu Tyr Tyr Leu 
                805                 810                 815     


Gln Asn Gly Arg Asp Met Tyr Val Asp Gln Glu Leu Asp Ile Asn Arg 
            820                 825                 830         


Leu Ser Asp Tyr Asp Val Asp Ala Ile Val Pro Gln Ser Phe Leu Lys 
        835                 840                 845             


Asp Asp Ser Ile Asp Asn Lys Val Leu Thr Arg Ser Asp Lys Asn Arg 
    850                 855                 860                 


Gly Lys Ser Asp Asn Val Pro Ser Glu Glu Val Val Lys Lys Met Lys 
865                 870                 875                 880 


Asn Tyr Trp Arg Gln Leu Leu Asn Ala Lys Leu Ile Thr Gln Arg Lys 
                885                 890                 895     


Phe Asp Asn Leu Thr Lys Ala Glu Arg Gly Gly Leu Ser Glu Leu Asp 
            900                 905                 910         


Lys Ala Gly Phe Ile Lys Arg Gln Leu Val Glu Thr Arg Gln Ile Thr 
        915                 920                 925             


Lys His Val Ala Gln Ile Leu Asp Ser Arg Met Asn Thr Lys Tyr Asp 
    930                 935                 940                 


Glu Asn Asp Lys Leu Ile Arg Glu Val Lys Val Ile Thr Leu Lys Ser 
945                 950                 955                 960 


Lys Leu Val Ser Asp Phe Arg Lys Asp Phe Gln Phe Tyr Lys Val Arg 
                965                 970                 975     


Glu Ile Asn Asn Tyr His His Ala His Asp Ala Tyr Leu Asn Ala Val 
            980                 985                 990         


Val Gly Thr Ala Leu Ile Lys Lys  Tyr Pro Lys Leu Glu  Ser Glu Phe 
        995                 1000                 1005             


Val Tyr  Gly Asp Tyr Lys Val  Tyr Asp Val Arg Lys  Met Ile Ala 
    1010                 1015                 1020             


Lys Ser  Glu Gln Glu Ile Gly  Lys Ala Thr Ala Lys  Tyr Phe Phe 
    1025                 1030                 1035             


Tyr Ser  Asn Ile Met Asn Phe  Phe Lys Thr Glu Ile  Thr Leu Ala 
    1040                 1045                 1050             


Asn Gly  Glu Ile Arg Lys Arg  Pro Leu Ile Glu Thr  Asn Gly Glu 
    1055                 1060                 1065             


Thr Gly  Glu Ile Val Trp Asp  Lys Gly Arg Asp Phe  Ala Thr Val 
    1070                 1075                 1080             


Arg Lys  Val Leu Ser Met Pro  Gln Val Asn Ile Val  Lys Lys Thr 
    1085                 1090                 1095             


Glu Val  Gln Thr Gly Gly Phe  Ser Lys Glu Ser Ile  Leu Pro Lys 
    1100                 1105                 1110             


Arg Asn  Ser Asp Lys Leu Ile  Ala Arg Lys Lys Asp  Trp Asp Pro 
    1115                 1120                 1125             


Lys Lys  Tyr Gly Gly Phe Asp  Ser Pro Thr Val Ala  Tyr Ser Val 
    1130                 1135                 1140             


Leu Val  Val Ala Lys Val Glu  Lys Gly Lys Ser Lys  Lys Leu Lys 
    1145                 1150                 1155             


Ser Val  Lys Glu Leu Leu Gly  Ile Thr Ile Met Glu  Arg Ser Ser 
    1160                 1165                 1170             


Phe Glu  Lys Asn Pro Ile Asp  Phe Leu Glu Ala Lys  Gly Tyr Lys 
    1175                 1180                 1185             


Glu Val  Lys Lys Asp Leu Ile  Ile Lys Leu Pro Lys  Tyr Ser Leu 
    1190                 1195                 1200             


Phe Glu  Leu Glu Asn Gly Arg  Lys Arg Met Leu Ala  Ser Ala Gly 
    1205                 1210                 1215             


Glu Leu  Gln Lys Gly Asn Glu  Leu Ala Leu Pro Ser  Lys Tyr Val 
    1220                 1225                 1230             


Asn Phe  Leu Tyr Leu Ala Ser  His Tyr Glu Lys Leu  Lys Gly Ser 
    1235                 1240                 1245             


Pro Glu  Asp Asn Glu Gln Lys  Gln Leu Phe Val Glu  Gln His Lys 
    1250                 1255                 1260             


His Tyr  Leu Asp Glu Ile Ile  Glu Gln Ile Ser Glu  Phe Ser Lys 
    1265                 1270                 1275             


Arg Val  Ile Leu Ala Asp Ala  Asn Leu Asp Lys Val  Leu Ser Ala 
    1280                 1285                 1290             


Tyr Asn  Lys His Arg Asp Lys  Pro Ile Arg Glu Gln  Ala Glu Asn 
    1295                 1300                 1305             


Ile Ile  His Leu Phe Thr Leu  Thr Asn Leu Gly Ala  Pro Ala Ala 
    1310                 1315                 1320             


Phe Lys  Tyr Phe Asp Thr Thr  Ile Asp Arg Lys Arg  Tyr Thr Ser 
    1325                 1330                 1335             


Thr Lys  Glu Val Leu Asp Ala  Thr Leu Ile His Gln  Ser Ile Thr 
    1340                 1345                 1350             


Gly Leu  Tyr Glu Thr Arg Ile  Asp Leu Ser Gln Leu  Gly Gly Asp 
    1355                 1360                 1365             


Glu Gly  Ala Asp Pro Lys Lys  Lys Arg Lys Val Asp  Pro Lys Lys 
    1370                 1375                 1380             


Lys Arg  Lys Val Asp Pro Lys  Lys Lys Arg Lys Val  Gly Ser Met 
    1385                 1390                 1395             


Pro Ala  Met Pro Ser Ser Gly  Pro Gly Asp Thr Ser  Ser Ser Ala 
    1400                 1405                 1410             


Ala Glu  Arg Glu Glu Asp Arg  Lys Asp Gly Glu Glu  Gln Glu Glu 
    1415                 1420                 1425             


Pro Arg  Gly Lys Glu Glu Arg  Gln Glu Pro Ser Thr  Thr Ala Arg 
    1430                 1435                 1440             


Lys Val  Gly Arg Pro Gly Arg  Lys Arg Lys His Pro  Pro Val Glu 
    1445                 1450                 1455             


Ser Gly  Asp Thr Pro Lys Asp  Pro Ala Val Ile Ser  Lys Ser Pro 
    1460                 1465                 1470             


Ser Met  Ala Gln Asp Ser Gly  Ala Ser Glu Leu Leu  Pro Asn Gly 
    1475                 1480                 1485             


Asp Leu  Glu Lys Arg Ser Glu  Pro Gln Pro Glu Glu  Gly Ser Pro 
    1490                 1495                 1500             


Ala Gly  Gly Gln Lys Gly Gly  Ala Pro Ala Glu Gly  Glu Gly Ala 
    1505                 1510                 1515             


Ala Glu  Thr Leu Pro Glu Ala  Ser Arg Ala Val Glu  Asn Gly Cys 
    1520                 1525                 1530             


Cys Thr  Pro Lys Glu Gly Arg  Gly Ala Pro Ala Glu  Ala Gly Lys 
    1535                 1540                 1545             


Glu Gln  Lys Glu Thr Asn Ile  Glu Ser Met Lys Met  Glu Gly Ser 
    1550                 1555                 1560             


Arg Gly  Arg Leu Arg Gly Gly  Leu Gly Trp Glu Ser  Ser Leu Arg 
    1565                 1570                 1575             


Gln Arg  Pro Met Pro Arg Leu  Thr Phe Gln Ala Gly  Asp Pro Tyr 
    1580                 1585                 1590             


Tyr Ile  Ser Lys Arg Lys Arg  Asp Glu Trp Leu Ala  Arg Trp Lys 
    1595                 1600                 1605             


Arg Glu  Ala Glu Lys Lys Ala  Lys Val Ile Ala Gly  Met Asn Ala 
    1610                 1615                 1620             


Val Glu  Glu Asn Gln Gly Pro  Gly Glu Ser Gln Lys  Val Glu Glu 
    1625                 1630                 1635             


Ala Ser  Pro Pro Ala Val Gln  Gln Pro Thr Asp Pro  Ala Ser Pro 
    1640                 1645                 1650             


Thr Val  Ala Thr Thr Pro Glu  Pro Val Gly Ser Asp  Ala Gly Asp 
    1655                 1660                 1665             


Lys Asn  Ala Thr Lys Ala Gly  Asp Asp Glu Pro Glu  Tyr Glu Asp 
    1670                 1675                 1680             


Gly Arg  Gly Phe Gly Ile Gly  Glu Leu Val Trp Gly  Lys Leu Arg 
    1685                 1690                 1695             


Gly Phe  Ser Trp Trp Pro Gly  Arg Ile Val Ser Trp  Trp Met Thr 
    1700                 1705                 1710             


Gly Arg  Ser Arg Ala Ala Glu  Gly Thr Arg Trp Val  Met Trp Phe 
    1715                 1720                 1725             


Gly Asp  Gly Lys Phe Ser Val  Val Cys Val Glu Lys  Leu Met Pro 
    1730                 1735                 1740             


Leu Ser  Ser Phe Cys Ser Ala  Phe His Gln Ala Thr  Tyr Asn Lys 
    1745                 1750                 1755             


Gln Pro  Met Tyr Arg Lys Ala  Ile Tyr Glu Val Leu  Gln Val Ala 
    1760                 1765                 1770             


Ser Ser  Arg Ala Gly Lys Leu  Phe Pro Val Cys His  Asp Ser Asp 
    1775                 1780                 1785             


Glu Ser  Asp Thr Ala Lys Ala  Val Glu Val Gln Asn  Lys Pro Met 
    1790                 1795                 1800             


Ile Glu  Trp Ala Leu Gly Gly  Phe Gln Pro Ser Gly  Pro Lys Gly 
    1805                 1810                 1815             


Leu Glu  Pro Pro Glu Glu Glu  Lys Asn Pro Tyr Lys  Glu Val Tyr 
    1820                 1825                 1830             


Thr Asp  Met Trp Val Glu Pro  Glu Ala Ala Ala Tyr  Ala Pro Pro 
    1835                 1840                 1845             


Pro Pro  Ala Lys Lys Pro Arg  Lys Ser Thr Ala Glu  Lys Pro Lys 
    1850                 1855                 1860             


Val Lys  Glu Ile Ile Asp Glu  Arg Thr Arg Glu Arg  Leu Val Tyr 
    1865                 1870                 1875             


Glu Val  Arg Gln Lys Cys Arg  Asn Ile Glu Asp Ile  Cys Ile Ser 
    1880                 1885                 1890             


Cys Gly  Ser Leu Asn Val Thr  Leu Glu His Pro Leu  Phe Val Gly 
    1895                 1900                 1905             


Gly Met  Cys Gln Asn Cys Lys  Asn Cys Phe Leu Glu  Cys Ala Tyr 
    1910                 1915                 1920             


Gln Tyr  Asp Asp Asp Gly Tyr  Gln Ser Tyr Cys Thr  Ile Cys Cys 
    1925                 1930                 1935             


Gly Gly  Arg Glu Val Leu Met  Cys Gly Asn Asn Asn  Cys Cys Arg 
    1940                 1945                 1950             


Cys Phe  Cys Val Glu Cys Val  Asp Leu Leu Val Gly  Pro Gly Ala 
    1955                 1960                 1965             


Ala Gln  Ala Ala Ile Lys Glu  Asp Pro Trp Asn Cys  Tyr Met Cys 
    1970                 1975                 1980             


Gly His  Lys Gly Thr Tyr Gly  Leu Leu Arg Arg Arg  Glu Asp Trp 
    1985                 1990                 1995             


Pro Ser  Arg Leu Gln Met Phe  Phe Ala Asn Asn His  Asp Gln Glu 
    2000                 2005                 2010             


Phe Asp  Pro Pro Lys Val Tyr  Pro Pro Val Pro Ala  Glu Lys Arg 
    2015                 2020                 2025             


Lys Pro  Ile Arg Val Leu Ser  Leu Phe Asp Gly Ile  Ala Thr Gly 
    2030                 2035                 2040             


Leu Leu  Val Leu Lys Asp Leu  Gly Ile Gln Val Asp  Arg Tyr Ile 
    2045                 2050                 2055             


Ala Ser  Glu Val Cys Glu Asp  Ser Ile Thr Val Gly  Met Val Arg 
    2060                 2065                 2070             


His Gln  Gly Lys Ile Met Tyr  Val Gly Asp Val Arg  Ser Val Thr 
    2075                 2080                 2085             


Gln Lys  His Ile Gln Glu Trp  Gly Pro Phe Asp Leu  Val Ile Gly 
    2090                 2095                 2100             


Gly Ser  Pro Cys Asn Asp Leu  Ser Ile Val Asn Pro  Ala Arg Lys 
    2105                 2110                 2115             


Gly Leu  Tyr Glu Gly Thr Gly  Arg Leu Phe Phe Glu  Phe Tyr Arg 
    2120                 2125                 2130             


Leu Leu  His Asp Ala Arg Pro  Lys Glu Gly Asp Asp  Arg Pro Phe 
    2135                 2140                 2145             


Phe Trp  Leu Phe Glu Asn Val  Val Ala Met Gly Val  Ser Asp Lys 
    2150                 2155                 2160             


Arg Asp  Ile Ser Arg Phe Leu  Glu Ser Asn Pro Val  Met Ile Asp 
    2165                 2170                 2175             


Ala Lys  Glu Val Ser Ala Ala  His Arg Ala Arg Tyr  Phe Trp Gly 
    2180                 2185                 2190             


Asn Leu  Pro Gly Met Asn Arg  Pro Leu Ala Ser Thr  Val Asn Asp 
    2195                 2200                 2205             


Lys Leu  Glu Leu Gln Glu Cys  Leu Glu His Gly Arg  Ile Ala Lys 
    2210                 2215                 2220             


Phe Ser  Lys Val Arg Thr Ile  Thr Thr Arg Ser Asn  Ser Ile Lys 
    2225                 2230                 2235             


Gln Gly  Lys Asp Gln His Phe  Pro Val Phe Met Asn  Glu Lys Glu 
    2240                 2245                 2250             


Asp Ile  Leu Trp Cys Thr Glu  Met Glu Arg Val Phe  Gly Phe Pro 
    2255                 2260                 2265             


Val His  Tyr Thr Asp Val Ser  Asn Met Ser Arg Leu  Ala Arg Gln 
    2270                 2275                 2280             


Arg Leu  Leu Gly Arg Ser Trp  Ser Val Pro Val Ile  Arg His Leu 
    2285                 2290                 2295             


Phe Ala  Pro Leu Lys Glu Tyr  Phe Ala Cys Val Glu  Phe Ala Tyr 
    2300                 2305                 2310             


Pro Tyr  Asp Val Pro Asp Tyr  Ala Ala Thr Asn Phe  Ser Leu Leu 
    2315                 2320                 2325             


Lys Gln  Ala Gly Asp Val Glu  Glu Asn Pro Gly Pro  Met Ser Glu 
    2330                 2335                 2340             


Leu Ile  Lys Glu Asn Met His  Met Lys Leu Tyr Met  Glu Gly Thr 
    2345                 2350                 2355             


Val Asp  Asn His His Phe Lys  Cys Thr Ser Glu Gly  Glu Gly Lys 
    2360                 2365                 2370             


Pro Tyr  Glu Gly Thr Gln Thr  Met Arg Ile Lys Val  Val Glu Gly 
    2375                 2380                 2385             


Gly Pro  Leu Pro Phe Ala Phe  Asp Ile Leu Ala Thr  Ser Phe Leu 
    2390                 2395                 2400             


Tyr Gly  Ser Lys Thr Phe Ile  Asn His Thr Gln Gly  Ile Pro Asp 
    2405                 2410                 2415             


Phe Phe  Lys Gln Ser Phe Pro  Glu Gly Phe Thr Trp  Glu Arg Val 
    2420                 2425                 2430             


Thr Thr  Tyr Glu Asp Gly Gly  Val Leu Thr Ala Thr  Gln Asp Thr 
    2435                 2440                 2445             


Ser Leu  Gln Asp Gly Cys Leu  Ile Tyr Asn Val Lys  Ile Arg Gly 
    2450                 2455                 2460             


Val Asn  Phe Thr Ser Asn Gly  Pro Val Met Gln Lys  Lys Thr Leu 
    2465                 2470                 2475             


Gly Trp  Glu Ala Phe Thr Glu  Thr Leu Tyr Pro Ala  Asp Gly Gly 
    2480                 2485                 2490             


Leu Glu  Gly Arg Asn Asp Met  Ala Leu Lys Leu Val  Gly Gly Ser 
    2495                 2500                 2505             


His Leu  Ile Ala Asn Ile Lys  Thr Thr Tyr Arg Ser  Lys Lys Pro 
    2510                 2515                 2520             


Ala Lys  Asn Leu Lys Met Pro  Gly Val Tyr Tyr Val  Asp Tyr Arg 
    2525                 2530                 2535             


Leu Glu  Arg Ile Lys Glu Ala  Asn Asn Glu Thr Tyr  Val Glu Gln 
    2540                 2545                 2550             


His Glu  Val Ala Val Ala Arg  Tyr Cys Asp Leu Pro  Ser Lys Leu 
    2555                 2560                 2565             


Gly His  Lys Leu Asn 
    2570             


<210>  162
<211>  579
<212>  DNA
<213>  Unknown

<220>
<223>  Daz1-Snrpn locus

<400>  162
cgactagaga gcaggccgcc tggccgggcc cccaccctct cagccacaga gaagggagtc       60

cgggtgcatg tggaaggcgc acctcatgcc tcacgggccg gcccgtcagc catcaggaca      120

gtcccgctcc cccccccccg cccccaaata aatgcccgaa atccggtaca aaggacacag      180

cagtgaccag accacacgag agaatatccc tccccgtgga atagggcagt gtgagcgtct      240

cgtaggctac agaaacaagc taggccagct gagagaacta gaacactcaa ggccagagaa      300

acggggccta cctacctaca gcagagccga gctgtagggt gcttggcaag acgctcaaat      360

ttccgcagta ggaatgctca agcattcctt ttggtagctg ccttttggca ggacattccg      420

gtcagaggga cagagacccc tgcattgcgg caaaaatgtg cgcatgtgca gccattgcct      480

gggacgcatg cgtagggagc cgcgcgacaa acctgagcca ttgcggcaag actagcgcag      540

agaggagagg gagccggaga tgccagacgc ttggttctg                             579


<210>  163
<211>  274
<212>  DNA
<213>  Unknown

<220>
<223>  Gapdh-Snrpn locus

<400>  163
ctacgtgcac ccgtaaagcc gcgagtagct gggcctctct ccaagacgct caaatttccg       60

cagtaggaat gctcaagcat tccttttggt agctgccttt tggcaggaca ttccggtcag      120

agggacagag acccctgcat tgcggcaaaa atgtgcgcat gtgcagccat tgcctgggac      180

gcatgcgtag ggagccgcgc gacaaacctg agccattgcg gcaagactag cgcagagagg      240

agagggagcc ggagatgcca gacgcttggt tctg                                  274


<210>  164
<211>  199
<212>  DNA
<213>  Unknown

<220>
<223>  BDNF IV locus

<400>  164
ctacaaagca tgcaatgccc tggaacggaa cctaataaaa gatgtatcat taaatgcgcg       60

gaactgactg gtaacgtgca ctagagtgtc tatcgaggca gaggaggtat catatgacag      120

ctcacgtcaa ggcagcgtgg agccctctcg tggactccca cccactccca caccgaggag      180

aggactgctc tcgctgccg                                                   199


<210>  165
<211>  253
<212>  DNA
<213>  Unknown

<220>
<223>  MyoD DE (DMR-5) locus

<400>  165
ccacagcatt tgggggcatt tatgggtctt cctataaact tctgagacag taattttatc       60

ctgcttcttt cggccaagta tcctcctcca gcagctggtc acaaagccag ttaatctccc      120

agagtgctca gcttaaaacc cgtgactcac aacacagcca gttgggggaa ggggacagcc      180

gcctccaaac gtggcgccca gactcagctg ttcctgggtc ttctccggtt tctctagctc      240

aggcctaggg ctg                                                         253


<210>  166
<211>  543
<212>  DNA
<213>  Unknown

<220>
<223>  CTCF miR290 locus

<400>  166
gttttgaggc ctcatttgta aggtgcctta tacctttagc cccagcccac ttttttttcc       60

ccctgctgta taaaattcag gtgtgagtac aatttttctt tttaaagatt tataagtgtt      120

ctgtagctat cttcagattc acccaaagaa ggcattggat cccattacag atgattgcaa      180

gccaccatgt agttgctggg aattgaactc aggacctctg acttaaccac tccagccctt      240

gagtacaatt tttgaaaaat taccttgtgg gtctttatgc tgtgacttgg ccagtagatg      300

gcagtcttgg tccatggaaa tgtctaggac tctggatatt tttccttttc tgtggtcttt      360

actgatcttc aaacctgcta accagccaat ccccgtccta aattatctgc gtggaatcta      420

catcaaaccc agtgagctcc atcaaaggtt gagtgtttag gtctcaagca gaacaatttt      480

gtcaacctgc acttactggg cctcctgacc taagacggtc ccatgtaaca ggatgacctt      540

gag                                                                    543


<210>  167
<211>  461
<212>  DNA
<213>  Unknown

<220>
<223>  CTCF Pou5f1 locus

<400>  167
tctcaccctt gatagtttga gggatatgag caaattacac ggttatcaga aggtggccat       60

agtgacactg aaaattggcc cattggcttc aaagatttac caaagtaccg tccgtatttt      120

ctacctacgg tgtgctggag cctagaggac actagggggc gcgctgagct cgcggaagcc      180

acccagagtc cttccaggag actcccttaa aggttgatca aattgttctt tgccaaactg      240

aatttatcat aaaaattata ctttattttg tattactttg tgtacatggg tgttttgcct      300

tcacagatgc gtctggtgca cctgagaagc cagaaaagag aacaggagtg aacaggtttg      360

tgggggctga cactcaaact cgaggactct gggaaagcat cgagtgctct taaccattga      420

gccatctctc cagcccatct gttttctttt gccggaggaa g                          461


