                         SEQUENCE LISTING

<110>  CALIMMUNE, INC.
 
<120>  METHODS AND COMPOSITIONS FOR TREATING CANINE CONDITIONS USING 
       RECOMBINANT SELF-COMPLEMENTARY ADENO-ASSOCIATED VIRUS

<130>  CALIM 16.04 PCT

<150>  62/377,346
<151>  2016-08-19

<160>  10    

<170>  PatentIn version 3.5

<210>  1
<211>  563
<212>  DNA
<213>  Canis familiaris

<400>  1
atcgatgata tcccaccatg gaaacctgca ggtgtcctct cagctaccta atctctttcc       60

tccttttcct gtcccattca gagacagcct gccgtccctt ggggaagaga ccttgcagga      120

tgcaagcctt cagaatctgg gatgttaacc agaagacctt ctacctgagg aataaccaac      180

tagtcgctgg atacttgcaa ggatcaaata ctaaattaga agagaagtta gatgtggtgc      240

ccgtcgagcc tcatgccgtg ttcttgggga tccatggggg gaagctgtgc ctggcctgtg      300

tcaagtctgg agatgagacc aggctccagc tggaggccgt taacatcact gacctgagta      360

agaacaagga tcaagacaag cgctttacct tcatcctctc agacagtggc cctaccacca      420

gctttgagtc tgctgcctgc cctggctggt tcctctgcac agcactggag gccgaccggc      480

ttgtcagcct caccaacaga ccagaagagg ccatgatggt cactaagttc tacttccaga      540

aggaataata gagatctact tga                                              563


<210>  2
<211>  1036
<212>  DNA
<213>  Canis familiaris

<400>  2
gtgactccga gatgcggtgt cccgttgcct cgctgtggcc accgaatgga aacctgcagg       60

tgtcctctca gctacctaat ctctttcctc cttttcctgt cccattcaga gacagcctgc      120

cgtcccttgg ggaagagacc ttgcaggatg caagccttca gaatctggga tgttaaccag      180

aagaccttct acctgaggaa taaccaacta gtcgctggat acttgcaagg atcaaatact      240

aaattagaag agaagttaga tgtggtgccc gtcgagcctc atgccgtgtt cttggggatc      300

catgggggga agctgtgcct ggcctgtgtc aagtctggag atgagaccag gctccagctg      360

gaggccgtta acatcactga cctgagtaag aacaaggatc aagacaagcg ctttaccttc      420

atcctctcag acagtggccc taccaccagc tttgagtctg ctgcctgccc tggctggttc      480

ctctgcacag cactggaggc cgaccggcct gtcagcctca ccaacagacc agaagaggcc      540

atgatggtca ctaagttcta cttccagaag gaataatagt gtgtccattc cgtgcttccc      600

ccccactccc aacacatcaa tgactccaga gatgcctctc cattctgcct ggggtctcct      660

ggctgtggtg gaggctctga ggagcagcct cggtggggtg gaccctcaga aggatgtatg      720

agagccctgg taacgggacc ctgcctccag cctcctcagc tagccaacct caatgctgcc      780

accacagtgg tctttctaaa gtgcacctct agctgcagca ctgctccagg cctttcaggg      840

ctgcctctgc cttctggatt aaagccaggc tgcttggcca gcctggcccc ctgctctcct      900

ctccgtaact ccttgctctc ctcccttgcc ccatgtccat gtcctggatc cctcctgccc      960

ctttgctggc ctcccaaacc ttgtgttttg caaaccgaaa aaaaaaaaaa aacgatccgg     1020

tacctctaga tcagaa                                                     1036


<210>  3
<211>  528
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Modified Canis familiaris IL-1Ra

<400>  3
atggagacct gcaggtgccc cctgagctac ctgatcagct tcctgctgtt cctgagccac       60

agcgagaccg cctgcaggcc cctgggcaag aggccctgca ggatgcaggc cttcaggatc      120

tgggacgtga accagaagac cttctacctg aggaacaacc agctggtggc cggctacctg      180

cagggcagca acaccaagct ggaggagaag ctggacgtgg tgcccgtgga gccccacgcc      240

gtgttcctgg gcatccacgg cggcaagctg tgcctggcct gcgtgaagag cggcgacgag      300

accaggctgc agctggaggc cgtgaacatc accgacctga gcaagaacaa ggaccaggac      360

aagaggttca ccttcatcct gagcgacagc ggccccacca ccagcttcga gagcgccgcc      420

tgccccggct ggttcctgtg caccgccctg gaggccgaca ggctggtgag cctgaccaac      480

aggcccgagg aggccatgat ggtgaccaag ttctacttcc agaaggag                   528


<210>  4
<211>  528
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Modified Canis familiaris IL-1Ra

<400>  4
atggagacct gccgctgccc cctgagctac ctgatcagct tcctgctgtt cctgagccac       60

agcgagaccg cctgccgccc cctgggcaag cgcccctgcc gcatgcaggc cttccgcatc      120

tgggacgtga accagaagac cttctacctg cgcaacaacc agctggtggc cggctacctg      180

cagggcagca acaccaagct ggaggagaag ctggacgtgg tgcccgtgga gccccacgcc      240

gtgttcctgg gcatccacgg cggcaagctg tgcctggcct gcgtgaagag cggcgacgag      300

acccgcctgc agctggaggc cgtgaacatc accgacctga gcaagaacaa ggaccaggac      360

aagcgcttca ccttcatcct gagcgacagc ggccccacca ccagcttcga gagcgccgcc      420

tgccccggct ggttcctgtg caccgccctg gaggccgacc gcctggtgag cctgaccaac      480

cgccccgagg aggccatgat ggtgaccaag ttctacttcc agaaggag                   528


<210>  5
<211>  528
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Modified IL-1Ra insert  (99% identical to SEQ ID NO: 2;

<400>  5
atggagacct gcaggtgccc actgagttac ctaatcagtt tcctcctgtt cctgagccac       60

agcgagaccg cctgcaggcc cctgggcaag aggccctgca ggatgcaggc cttcaggatc      120

tgggacgtga accagaagac cttctacctg aggaacaacc agctggtggc cggctacctg      180

cagggcagca acaccaagct ggaggagaag ctggacgtgg tgcccgtgga gccccacgcc      240

gtgttcctgg gcatccacgg cggcaagctg tgcctggcct gcgtgaagag cggcgacgag      300

accaggctgc agctggaggc cgtgaacatc accgacctga gcaagaacaa ggaccaggac      360

aagaggttca ccttcatcct gagcgacagc ggccccacca ccagcttcga gagcgccgcc      420

tgccccggct ggttcctgtg caccgccctg gaggccgaca ggctggtgag cctgaccaac      480

aggcccgagg aggccatgat ggtgaccaag ttctacttcc agaaggag                   528


<210>  6
<211>  528
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Modified IL-1Ra insert  (98% identical to SEQ ID NO: 2;

<400>  6
atggagacct gcaggtgccc actgagttac ctaatcagtt tcctcctgtt cctgagccac       60

agcgagaccg cctgcaggcc cctgggcaag aggccctgca ggatgcaggc cttcaggatc      120

tgggacgtga accagaagac cttctacctg aggaacaacc agctggtggc cggctacctg      180

cagggcagca acaccaagct ggaggagaag ctggacgtgg tgcccgtgga gccccacgcc      240

gtgttcctgg gcatccacgg cggcaagctg tgcctggcct gcgtgaagag cggcgacgag      300

accaggctgc agctggaggc cgtgaacatc accgacctga gcaagaacaa ggaccaggac      360

aagaggttca ccttcatcct gagcgacagc ggccccacca ccagcttcga gagcgccgcc      420

tgccccggct ggttcctgtg caccgccctg gaggccgaca ggctggtgag cctgaccaac      480

aggcccgagg aggcaatgat ggtaaccaaa ttctatttcc ataaggaa                   528


<210>  7
<211>  528
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Modified IL-1Ra insert  (95% identical to SEQ ID NO: 2;

<400>  7
atggagacct gcaggtgccc actgagttac ctaatcagtt tcctcctgtt cctgagccac       60

agcgagaccg cctgcaggcc cctgggcaag aggccctgta ggatgcaggc cttcaggatc      120

tgggatgtga accagaagac cttttacctg aggaataacc agctggtagc cggctacctg      180

caggggagca acaccaagct ggaggagaag ctggacgtgg tgcccgtgga gccccacgcc      240

gtgtttctgg gcatccacgg aggcaagctg tgcctagcct gcgtgaagag cggcgacgag      300

accaggctgc agctggaggc ggtgaacatc accgacctga gcaagaacaa agaccaggac      360

aagaggttca ccttcatcct aagcgacagc ggccccacca ccagcttcga aagcgccgct      420

tgccccggct ggttcctctg caccgccctg gaggccgaca ggctggtgag cctgaccaac      480

aggcccgagg aggcaatgat ggtaaccaaa ttctatttcc ataaggaa                   528


<210>  8
<211>  176
<212>  PRT
<213>  Canis familiaris

<400>  8

Met Glu Thr Cys Arg Cys Pro Leu Ser Tyr Leu Ile Ser Phe Leu Leu 
1               5                   10                  15      


Phe Leu Ser His Ser Glu Thr Ala Cys Arg Pro Leu Gly Lys Arg Pro 
            20                  25                  30          


Cys Arg Met Gln Ala Phe Arg Ile Trp Asp Val Asn Gln Lys Thr Phe 
        35                  40                  45              


Tyr Leu Arg Asn Asn Gln Leu Val Ala Gly Tyr Leu Gln Gly Ser Asn 
    50                  55                  60                  


Thr Lys Leu Glu Glu Lys Leu Asp Val Val Pro Val Glu Pro His Ala 
65                  70                  75                  80  


Val Phe Leu Gly Ile His Gly Gly Lys Leu Cys Leu Ala Cys Val Lys 
                85                  90                  95      


Ser Gly Asp Glu Thr Arg Leu Gln Leu Glu Ala Val Asn Ile Thr Asp 
            100                 105                 110         


Leu Ser Lys Asn Lys Asp Gln Asp Lys Arg Phe Thr Phe Ile Leu Ser 
        115                 120                 125             


Asp Ser Gly Pro Thr Thr Ser Phe Glu Ser Ala Ala Cys Pro Gly Trp 
    130                 135                 140                 


Phe Leu Cys Thr Ala Leu Glu Ala Asp Arg Leu Val Ser Leu Thr Asn 
145                 150                 155                 160 


Arg Pro Glu Glu Ala Met Met Val Thr Lys Phe Tyr Phe Gln Lys Glu 
                165                 170                 175     


<210>  9
<211>  176
<212>  PRT
<213>  Canis familiaris

<400>  9

Met Glu Thr Cys Arg Cys Pro Leu Ser Tyr Leu Ile Ser Phe Leu Leu 
1               5                   10                  15      


Phe Leu Ser His Ser Glu Thr Ala Cys Arg Pro Leu Gly Lys Arg Pro 
            20                  25                  30          


Cys Arg Met Gln Ala Phe Arg Ile Trp Asp Val Asn Gln Lys Thr Phe 
        35                  40                  45              


Tyr Leu Arg Asn Asn Gln Leu Val Ala Gly Tyr Leu Gln Gly Ser Asn 
    50                  55                  60                  


Thr Lys Leu Glu Glu Lys Leu Asp Val Val Pro Val Glu Pro His Ala 
65                  70                  75                  80  


Val Phe Leu Gly Ile His Gly Gly Lys Leu Cys Leu Ala Cys Val Lys 
                85                  90                  95      


Ser Gly Asp Glu Thr Arg Leu Gln Leu Glu Ala Val Asn Ile Thr Asp 
            100                 105                 110         


Leu Ser Lys Asn Lys Asp Gln Asp Lys Arg Phe Thr Phe Ile Leu Ser 
        115                 120                 125             


Asp Ser Gly Pro Thr Thr Ser Phe Glu Ser Ala Ala Cys Pro Gly Trp 
    130                 135                 140                 


Phe Leu Cys Thr Ala Leu Glu Ala Asp Arg Pro Val Ser Leu Thr Asn 
145                 150                 155                 160 


Arg Pro Glu Glu Ala Met Met Val Thr Lys Phe Tyr Phe Gln Lys Glu 
                165                 170                 175     


<210>  10
<211>  1165
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  IL-1 beta/
IL-6 hybrid promoter

<400>  10
atccaagagg gagaagaagc ccattggaga tgatgccata aaggaagtgg aagcgatatg       60

ataaaaatca tagtgcccat tcccaaataa tcccagaagc agaagggaaa ggagagaaat      120

atccacaaag acaggtgtgg gtacacacaa catttttcat actttaagat cccagaggac      180

tcatggaaat gatacaagaa aatgactcat aagaacaaat attaggaagc cagtgccaag      240

aatgagatgg gaaattgggg aaaatgttgg gggcagattg cttagttctg ttctaagcaa      300

gagggtgaac aaggaaggaa cagctcacta caaagaacag acatcactgc atgtacacac      360

aataatataa gaactaaccc atgattattt tgcttgtctt cttgttcaaa atgattgaag      420

accaatgaga tgagatcaac cttgataact ggctggcttc ggcatgatta gacacaagat      480

ggtatcaggg cacttgctgc tttgaataat gtcagtctcc tgtcttggaa gaatgacctg      540

acagggtaaa gaggaacttg cagctgagaa aggctttagt gactcaagag ctgaataatt      600

ccccaaaagc tggagcatcc tggcatttcc agctccccat ctctgcttgt tccacttcct      660

tggggctaca tcaccatcta catcatcatc actcttccac tccctccctt agtgccaact      720

atgtttatag cgagatattt tctgctcatt ggggatcgga aggaagtgct gtggcctgag      780

cggtctcctt gggaagacag gatctgatac atacgttgca caacctattt gacataagag      840

gtttcacttc ctgagatgga tgggatggta gcagatttgg gtccaggtta cagggccagg      900

atgagacatg gcagaactgt ggagactgtt acgtcagggg gcattgcccc atggctccaa      960

aatttccctc gagcctctgg ccccaccctc accctccaac aaagatttat caaatgtggg     1020

attttcccat gagtctcaat attagagtct caacccccaa taaatatagg actggagatg     1080

tctgaggctc attctgccct cgagcccacc gggaacgaaa gagaagctct atctcccctc     1140

caggagccca gctatgaact ccttc                                           1165


