                         SEQUENCE LISTING

<110>  CALIMMUNE, INC.
 
<120>  METHODS AND COMPOSITIONS FOR TREATING CONDITIONS USING 
       RECOMBINANT SELF-COMPLEMENTARY ADENO-ASSOCIATED VIRUS

<130>  CALIM 16.02 PCT

<150>  62/377,297
<151>  2016-08-19

<160>  7     

<170>  PatentIn version 3.5

<210>  1
<211>  5718
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Sequence of Hu-IL-1Ra plasmid containing entire viral sequence 
       with modified human IL1Ra insert and the SacII / NotI restriction
       sites.

<400>  1
cattaatgaa tcggccaacg cgcggggaga ggcggtttgc gtattgggcg ctcttccgct       60

tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac      120

tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga      180

gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat      240

aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac      300

ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct      360

gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg      420

ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg      480

ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt      540

cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg      600

attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac      660

ggctacacta gaaggacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga      720

aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt      780

gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt      840

tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga      900

ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc      960

taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct     1020

atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata     1080

actacgatac gggagggctt accatctggc cccagtgctg caatgatacc gcgagaccca     1140

cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga     1200

agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga     1260

gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctac aggcatcgtg     1320

gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga     1380

gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt     1440

gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct     1500

cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca     1560

ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat acgggataat     1620

accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga     1680

aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc     1740

aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg     1800

caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc     1860

ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt     1920

gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca     1980

cctgacgtct aagaaaccat tattatcatg acattaacct ataaaaatag gcgtatcacg     2040

aggccctttc gtctcgcgcg tttcggtgat gacggtgaaa acctctgaca catgcagctc     2100

ccggagacgg tcacagcttg tctgtaagcg gatgccggga gcagacaagc ccgtcagggc     2160

gcgtcagcgg gtgttggcgg gtgtcggggc tggcttaact atgcggcatc agagcagatt     2220

gtactgagag tgcaccatat gcggtgtgaa ataccgcaca gatgcgtaag gagaaaatac     2280

cgcatcagga attccaacat ccaataaatc atacaggcaa ggcaaagaat tagcaaaatt     2340

aagcaataaa gcctcagagc ataaagctaa atcggttgta ccaaaaacat tatgaccctg     2400

taatactttt gcgggagaag cctttatttc aacgcaagga taaaaatttt tagaaccctc     2460

atatatttta aatgcaatgc ctgagtaatg tgtaggtaaa gattcaaacg ggtgagaaag     2520

gccggagaca gtcaaatcac catcaatatg atattcaacc gttctagctg ataaattcat     2580

gccggagagg gtagctattt ttgagaggtc tctacaaagg ctatcaggtc attgcctgag     2640

agtctggagc aaacaagaga atcgatgaac ggtaatcgta aaactagcat gtcaatcata     2700

tgtaccccgg ttgataatca gaaaagcccc aaaaacagga agattgtata agcaaatatt     2760

taaattgtaa acgttaatat tttgttaaaa ttcgcgttaa atttttgtta aatcagctca     2820

ttttttaacc aataggccga aatcggcaaa atcccttata aatcaaaaga atagaccgag     2880

atagggttga gtgttgttcc agtttggaac aagagtccac tattaaagaa cgtggactcc     2940

aacgtcaaag ggcgaaaaac cgtctatcag ggcgatggcc cactacgtga accatcaccc     3000

taatcaagtt ttttggggtc gaggtgccgt aaagcactaa atcggaaccc taaagggagc     3060

ccccgattta gagcttgacg gggaaagccg gcgaacgtgg cgagaaagga agggaagaaa     3120

gcgaaaggag cgggcgctag ggcgctggca agtgtagcgg tcacgctgcg cgtaaccacc     3180

acacccgccg cgcttaatgc gccgctacag ggcgcgtact atggttgctt tgacgagcac     3240

gtataacgtg ctttcctcgt tagaatcaga gcgggagcta aacaggaggc cgattaaagg     3300

gattttagac aggaacggta cgccagaatc ctgagaagtg tttttataat cagtgaggcc     3360

accgagtaaa agagtctgtc catcacgcaa attaaccgtt gtcgcaatac ttctttgatt     3420

agtaataaca tcacttgcct gagtagaaga actcaaacta tcggccttgc tggtaatatc     3480

cagaacaata ttaccgccag ccattgcaac aggaaaaacg ctcatggaaa tacctacatt     3540

ttgacgctca atcgtctgga attccattcg ccattcaggc tgcgcaactg ttgggaaggg     3600

cgatcggtgc gggcctcttc gctattacgc cagctggcgc gctcgctcgc tcactgaggc     3660

cgcccgggca aagcccgggc gtcgggcgac ctttggtcgc ccggcctcag tgagcgagcg     3720

agcgcgcaga gagggagtgg ccaactccat cactaggggt tccttgtagt taatgattaa     3780

cccgccatgc tacttatcta cgtagccatg ctcgatctga attcggtacc cgttacataa     3840

cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt gacgtcaata     3900

atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca atgggtggag     3960

tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc aagtacgccc     4020

cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta catgacctta     4080

tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac catggtgatg     4140

cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg atttccaagt     4200

ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg ggactttcca     4260

aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt acggtgggag     4320

gtctatataa gcagagctcg tttagtgaac cgtcagatcg cctggagacg ccatccacgc     4380

tgttttgacc tccatagaag acaccgggac cgatccagcc tccggactct agaggatccg     4440

gtactcgagg aactgaaaaa ccagaaagtt aactggtaag tttagtcttt ttgtctttta     4500

tttcaggtcc cggatccggt ggtggtgcaa atcaaagaac tgctcctcag tggatgttgc     4560

ctttacttct aggcctgtac ggaagtgtta cttctgctct aaaagctgcg gaattgtacc     4620

cgcgggccac catggaaatc tgcagaggcc tgcggagcca cctgattacc ctgctgctgt     4680

tcctgttcca cagcgagaca atctgccggc ccagcggccg gaagtccagc aagatgcagg     4740

ccttccggat ctgggacgtg aaccagaaaa ccttctacct gcggaacaac cagctggtgg     4800

ccggatacct gcagggcccc aacgtgaacc tggaagagaa gatcgacgtg gtgcccatcg     4860

agccccacgc cctgtttctg ggcatccacg gcggcaagat gtgcctgagc tgcgtgaagt     4920

ccggcgacga gacaagactg cagctggaag ccgtgaacat caccgacctg agcgagaacc     4980

ggaagcagga caagagattc gccttcatca gaagcgacag cggccccacc accagctttg     5040

agagcgccgc ctgccccggc tggttcctgt gtacagccat ggaagccgac cagcccgtgt     5100

ccctgacaaa catgcccgac gagggcgtga tggtcaccaa gttctatttt caagaagatg     5160

agtaataagc ggccgccggg atccagacat gataagatac attgatgagt ttggacaaac     5220

cacaactaga atgcagtgaa aaaaatgctt tatttgtgaa atttgtgatg ctattgcttt     5280

atttgtaacc attataagct gcaataaaca agttaacaac aacaattgca ttcattttat     5340

gtttcaggtt cagggggagg tgtgggaggt tttttagtcg actagagctc gctgatcagc     5400

ctcgactgtg ccttctagtt gccagccatc tgttgtttgc ccctcccccg tgccttcctt     5460

gaccctggaa ggtgccactc ccactgtcct ttcctaataa aatgaggaaa ttgcatcgca     5520

ttgtctgagt aggtgtcatt ctattctggg gggtggggtg gggcaggaca gcaaggggga     5580

ggattgggaa gacaatagca ggaaccccac tccctctctg cgcgctcgct cgctcactga     5640

ggccgggcga ccaaaggtcg cccgacgccc gggctttgcc cgggcggcct cagtgagcga     5700

gcgagcgcgc agctgctg                                                   5718


<210>  2
<211>  537
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Modified human IL-1Ra insert

<400>  2
atggaaatct gcagaggcct gcggagccac ctgattaccc tgctgctgtt cctgttccac       60

agcgagacaa tctgccggcc cagcggccgg aagtccagca agatgcaggc cttccggatc      120

tgggacgtga accagaaaac cttctacctg cggaacaacc agctggtggc cggatacctg      180

cagggcccca acgtgaacct ggaagagaag atcgacgtgg tgcccatcga gccccacgcc      240

ctgtttctgg gcatccacgg cggcaagatg tgcctgagct gcgtgaagtc cggcgacgag      300

acaagactgc agctggaagc cgtgaacatc accgacctga gcgagaaccg gaagcaggac      360

aagagattcg ccttcatcag aagcgacagc ggccccacca ccagctttga gagcgccgcc      420

tgccccggct ggttcctgtg tacagccatg gaagccgacc agcccgtgtc cctgacaaac      480

atgcccgacg agggcgtgat ggtcaccaag ttctattttc aagaagatga gtaataa         537


<210>  3
<211>  537
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Modified human IL-1Ra insert  (98% identical to SEQ ID NO: 2;

<400>  3
atggaaatct gcagaggact gcggagccac ctaattaccc tactcctttt cctgttccac       60

agcgagacaa tctgccggcc cagcggccgg aagtccagca agatgcaggc tttccggatc      120

tgggacgtga accagaaaac cttctacctc cggaacaacc agctggtggc gggatacctc      180

cagggcccca acgtgaacct agaagagaag atcgacgtgg tgcccatcga gccccacgcc      240

ctgtttctgg gcatccacgg cggcaagatg tgcctgagct gcgtgaagtc cggcgacgag      300

acaagactgc agctggaagc cgtgaacatc accgacctga gcgagaaccg gaagcaggac      360

aagagattcg ccttcatcag aagcgacagc ggccccacca ccagctttga gagcgccgcc      420

tgccccggct ggttcctgtg tacagccatg gaagccgacc agcccgtgtc cctgacaaac      480

atgcccgacg agggcgtgat ggtcaccaag ttctattttc aagaagatga gtaataa         537


<210>  4
<211>  537
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Modified human IL-1Ra insert  (99% identical to SEQ ID NO: 2;

<400>  4
atggagatct gcagaggcct gcggagccat ctgattaccc tactgctttt cctgttccat       60

agcgagacaa tctgccggcc cagcggccgg aagtccagca aaatgcaggc cttccggatc      120

tgggacgtga accagaaaac cttctacctg cggaacaacc agctggtggc cggatacctg      180

cagggcccca acgtgaacct ggaagagaag atcgacgtgg tgcccatcga gccccacgcc      240

ctgtttctgg gcatccacgg cggcaagatg tgcctgagct gcgtgaagtc cggcgacgag      300

acaagactgc agctggaagc cgtgaacatc accgacctga gcgagaaccg gaagcaggac      360

aagagattcg ccttcatcag aagcgacagc ggccccacca ccagctttga gagcgccgcc      420

tgccccggct ggttcctgtg tacagccatg gaagccgacc agcccgtgtc cctgacaaac      480

atgcccgacg agggcgtgat ggtcaccaag ttctattttc aagaagatga gtaataa         537


<210>  5
<211>  537
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Modified human IL-1Ra insert  (95% identical to SEQ ID NO: 2;

<400>  5
atggagatct gcagaggact gcggagccac ctaattaccc tactcctttt cctgttccat       60

agcgagacaa tctgccggcc cagcggccgg aagtccagca aaatgcaggc tttccggatc      120

tgggatgtga accagaagac cttctacctc cggaacaacc agctggtggc gggatacctc      180

cagggcccca acgtgaacct agaagagaag atcgacgtgg tgcccatcga gccccacgcc      240

ctgtttctgg gcatccatgg cggcaagatg tgtctgagtt gcgtgaagtc aggcgacgag      300

acaagactgc agctggaagc cgtgaacatc accgacctga gcgaaaaccg gaagcaggac      360

aagagattcg ccttcatcag aagcgacagc ggccccacca ctagctttga gagcgcagcc      420

tgccccggct ggttcctgtg tacagccatg gaggccgacc agcccgtgtc cctgacaaac      480

atgcctgacg aaggcgtgat ggtcaccaag ttctactttc aagaagatga ataataa         537


<210>  6
<211>  177
<212>  PRT
<213>  Homo sapiens

<400>  6

Met Glu Ile Cys Arg Gly Leu Arg Ser His Leu Ile Thr Leu Leu Leu 
1               5                   10                  15      


Phe Leu Phe His Ser Glu Thr Ile Cys Arg Pro Ser Gly Arg Lys Ser 
            20                  25                  30          


Ser Lys Met Gln Ala Phe Arg Ile Trp Asp Val Asn Gln Lys Thr Phe 
        35                  40                  45              


Tyr Leu Arg Asn Asn Gln Leu Val Ala Gly Tyr Leu Gln Gly Pro Asn 
    50                  55                  60                  


Val Asn Leu Glu Glu Lys Ile Asp Val Val Pro Ile Glu Pro His Ala 
65                  70                  75                  80  


Leu Phe Leu Gly Ile His Gly Gly Lys Met Cys Leu Ser Cys Val Lys 
                85                  90                  95      


Ser Gly Asp Glu Thr Arg Leu Gln Leu Glu Ala Val Asn Ile Thr Asp 
            100                 105                 110         


Leu Ser Glu Asn Arg Lys Gln Asp Lys Arg Phe Ala Phe Ile Arg Ser 
        115                 120                 125             


Asp Ser Gly Pro Thr Thr Ser Phe Glu Ser Ala Ala Cys Pro Gly Trp 
    130                 135                 140                 


Phe Leu Cys Thr Ala Met Glu Ala Asp Gln Pro Val Ser Leu Thr Asn 
145                 150                 155                 160 


Met Pro Asp Glu Gly Val Met Val Thr Lys Phe Tyr Phe Gln Glu Asp 
                165                 170                 175     


Glu 
    


<210>  7
<211>  1165
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  IL-1 beta/
IL-6 hybrid promoter

<400>  7
atccaagagg gagaagaagc ccattggaga tgatgccata aaggaagtgg aagcgatatg       60

ataaaaatca tagtgcccat tcccaaataa tcccagaagc agaagggaaa ggagagaaat      120

atccacaaag acaggtgtgg gtacacacaa catttttcat actttaagat cccagaggac      180

tcatggaaat gatacaagaa aatgactcat aagaacaaat attaggaagc cagtgccaag      240

aatgagatgg gaaattgggg aaaatgttgg gggcagattg cttagttctg ttctaagcaa      300

gagggtgaac aaggaaggaa cagctcacta caaagaacag acatcactgc atgtacacac      360

aataatataa gaactaaccc atgattattt tgcttgtctt cttgttcaaa atgattgaag      420

accaatgaga tgagatcaac cttgataact ggctggcttc ggcatgatta gacacaagat      480

ggtatcaggg cacttgctgc tttgaataat gtcagtctcc tgtcttggaa gaatgacctg      540

acagggtaaa gaggaacttg cagctgagaa aggctttagt gactcaagag ctgaataatt      600

ccccaaaagc tggagcatcc tggcatttcc agctccccat ctctgcttgt tccacttcct      660

tggggctaca tcaccatcta catcatcatc actcttccac tccctccctt agtgccaact      720

atgtttatag cgagatattt tctgctcatt ggggatcgga aggaagtgct gtggcctgag      780

cggtctcctt gggaagacag gatctgatac atacgttgca caacctattt gacataagag      840

gtttcacttc ctgagatgga tgggatggta gcagatttgg gtccaggtta cagggccagg      900

atgagacatg gcagaactgt ggagactgtt acgtcagggg gcattgcccc atggctccaa      960

aatttccctc gagcctctgg ccccaccctc accctccaac aaagatttat caaatgtggg     1020

attttcccat gagtctcaat attagagtct caacccccaa taaatatagg actggagatg     1080

tctgaggctc attctgccct cgagcccacc gggaacgaaa gagaagctct atctcccctc     1140

caggagccca gctatgaact ccttc                                           1165


