                         SEQUENCE LISTING

<110>  The BROAD Institute, Inc.
       Massachusetts Institute of Technology
       Zhang, Feng
       Scott, David Arthur
       Yan, Winston Xia
       Choudhury, Sourav
       Heidenreich, Matthias
 
<120>  Novel CRISPR Enzymes and Systems

<130>  BROD-0971WP

<140>  PCT/US2017/047458
<141>  2017-08-17

<150>  US 62/437,031
<151>  2016-12-20

<150>  US 62/376,372
<151>  2016-08-17

<160>  72    

<170>  PatentIn version 3.5

<210>  1
<211>  7
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic

<400>  1

Glu Asn Leu Tyr Phe Gln Gly 
1               5           


<210>  2
<211>  7
<212>  PRT
<213>  SV40

<400>  2

Pro Lys Lys Lys Arg Lys Val 
1               5           


<210>  3
<211>  16
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic

<400>  3

Lys Arg Pro Ala Ala Thr Lys Lys Ala Gly Gln Ala Lys Lys Lys Lys 
1               5                   10                  15      


<210>  4
<211>  9
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic

<400>  4

Pro Ala Ala Lys Arg Val Lys Leu Asp 
1               5                   


<210>  5
<211>  11
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic

<400>  5

Arg Gln Arg Arg Asn Glu Leu Lys Arg Ser Pro 
1               5                   10      


<210>  6
<211>  38
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic

<400>  6

Asn Gln Ser Ser Asn Phe Gly Pro Met Lys Gly Gly Asn Phe Gly Gly 
1               5                   10                  15      


Arg Ser Ser Gly Pro Tyr Gly Gly Gly Gly Gln Tyr Phe Ala Lys Pro 
            20                  25                  30          


Arg Asn Gln Gly Gly Tyr 
        35              


<210>  7
<211>  42
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic

<400>  7

Arg Met Arg Ile Glx Phe Lys Asn Lys Gly Lys Asp Thr Ala Glu Leu 
1               5                   10                  15      


Arg Arg Arg Arg Val Glu Val Ser Val Glu Leu Arg Lys Ala Lys Lys 
            20                  25                  30          


Asp Glu Gln Ile Leu Lys Arg Arg Asn Val 
        35                  40          


<210>  8
<211>  8
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic

<400>  8

Val Ser Arg Lys Arg Pro Arg Pro 
1               5               


<210>  9
<211>  8
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic

<400>  9

Pro Pro Lys Lys Ala Arg Glu Asp 
1               5               


<210>  10
<211>  8
<212>  PRT
<213>  Homo sapiens

<400>  10

Pro Gln Pro Lys Lys Lys Pro Leu 
1               5               


<210>  11
<211>  12
<212>  PRT
<213>  Mus

<400>  11

Ser Ala Leu Ile Lys Lys Lys Lys Lys Met Ala Pro 
1               5                   10          


<210>  12
<211>  5
<212>  PRT
<213>  Influenza NS1

<400>  12

Asp Arg Leu Arg Arg 
1               5   


<210>  13
<211>  7
<212>  PRT
<213>  Influenza NS1

<400>  13

Pro Lys Gln Lys Lys Arg Lys 
1               5           


<210>  14
<211>  10
<212>  PRT
<213>  Hepatitis virus

<400>  14

Arg Lys Leu Lys Lys Lys Ile Lys Lys Leu 
1               5                   10  


<210>  15
<211>  10
<212>  PRT
<213>  Mus

<400>  15

Arg Glu Lys Lys Lys Phe Leu Lys Arg Arg 
1               5                   10  


<210>  16
<211>  20
<212>  PRT
<213>  Homo sapiens

<400>  16

Lys Arg Lys Gly Asp Glu Val Asp Gly Val Asp Glu Val Ala Lys Lys 
1               5                   10                  15      


Lys Ser Lys Lys 
            20  


<210>  17
<211>  17
<212>  PRT
<213>  Homo sapiens

<400>  17

Arg Lys Cys Leu Gln Ala Gly Met Asn Leu Glu Ala Arg Lys Thr Lys 
1               5                   10                  15      


Lys 
    


<210>  18
<211>  4
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic

<400>  18

Gly Gly Gly Ser 
1               


<210>  19
<211>  15
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic

<400>  19

Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser 
1               5                   10                  15  


<210>  20
<211>  30
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic

<400>  20

Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly 
1               5                   10                  15      


Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser 
            20                  25                  30  


<210>  21
<211>  45
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic

<400>  21

Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly 
1               5                   10                  15      


Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly 
            20                  25                  30          


Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser 
        35                  40                  45  


<210>  22
<211>  60
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic

<400>  22

Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly 
1               5                   10                  15      


Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly 
            20                  25                  30          


Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly 
        35                  40                  45              


Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser 
    50                  55                  60  


<210>  23
<211>  6
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic

<400>  23
gccacc                                                                   6


<210>  24
<211>  6
<212>  DNA
<213>  Unknown

<220>
<223>  unknown

<400>  24
ttgaat                                                                   6


<210>  25
<211>  6
<212>  DNA
<213>  Unknown

<220>
<223>  unknown

<400>  25
ttgggt                                                                   6


<210>  26
<211>  9
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic CRI Variant

<400>  26

Leu Ala Gly Leu Ile Asp Ala Asp Gly 
1               5                   


<210>  27
<211>  6
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic

<400>  27
gccacc                                                                   6


<210>  28
<211>  5
<212>  PRT
<213>  Unknown

<220>
<223>  Unknown

<400>  28

Ala Pro Arg Pro Gly 
1               5   


<210>  29
<211>  12
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic

<400>  29

Phe Trp Tyr His Lys Met Ile Leu Val Ala Gly Cys 
1               5                   10          


<210>  30
<211>  12
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic

<400>  30

Trp Tyr His Lys Arg Glu Asp Cys Ser Thr Asn Gln 
1               5                   10          


<210>  31
<211>  9
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic

<400>  31

Val Cys Ala Gly Ser Pro Thr Asn Asp 
1               5                   


<210>  32
<211>  7
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic

<400>  32

Ala Gly Ala Ala Ala Lys Ala 
1               5           


<210>  33
<211>  5
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic

<400>  33

Gly Gly Gly Gly Ser 
1               5   


<210>  34
<211>  10
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic

<400>  34

Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser 
1               5                   10  


<210>  35
<211>  20
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic

<400>  35

Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly 
1               5                   10                  15      


Gly Gly Gly Ser 
            20  


<210>  36
<211>  25
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic

<400>  36

Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly 
1               5                   10                  15      


Gly Gly Gly Ser Gly Gly Gly Gly Ser 
            20                  25  


<210>  37
<211>  35
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic

<400>  37

Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly 
1               5                   10                  15      


Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly 
            20                  25                  30          


Gly Gly Ser 
        35  


<210>  38
<211>  40
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic

<400>  38

Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly 
1               5                   10                  15      


Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly 
            20                  25                  30          


Gly Gly Ser Gly Gly Gly Gly Ser 
        35                  40  


<210>  39
<211>  50
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic

<400>  39

Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly 
1               5                   10                  15      


Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly 
            20                  25                  30          


Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly 
        35                  40                  45              


Gly Ser 
    50  


<210>  40
<211>  55
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic

<400>  40

Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly 
1               5                   10                  15      


Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly 
            20                  25                  30          


Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly 
        35                  40                  45              


Gly Ser Gly Gly Gly Gly Ser 
    50                  55  


<210>  41
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic

<400>  41
gagtccgagc agaagaagaa                                                   20


<210>  42
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic

<400>  42
gagtcctagc aggagaagaa                                                   20


<210>  43
<211>  20
<212>  DNA
<213>  Homo sapiens

<400>  43
gccaaattgg acgaccctcg                                                   20


<210>  44
<211>  20
<212>  DNA
<213>  Homo sapiens

<400>  44
cgaggagacc cccgtttcgg                                                   20


<210>  45
<211>  20
<212>  DNA
<213>  Homo sapiens

<400>  45
cccgccgccg ccgtggctcg                                                   20


<210>  46
<211>  20
<212>  DNA
<213>  Homo sapiens

<400>  46
tgagctctac gagatccaca                                                   20


<210>  47
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic primer

<400>  47
ggtctcatgt gtggcactca                                                   20


<210>  48
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic primer

<400>  48
tgtccaacct tcaggcaagg                                                   20


<210>  49
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic primer

<400>  49
gtaacgtcct ggacactgtg g                                                 21


<210>  50
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic primer

<400>  50
ttggtccaat aggtcatgac g                                                 21


<210>  51
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic primer

<400>  51
tggctaagcc tggccaagaa cg                                                22


<210>  52
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic primer

<400>  52
tcaggatgaa ggctgccttc gg                                                22


<210>  53
<211>  485
<212>  DNA
<213>  Homo sapiens

<400>  53
gtgtctagac tgcagagggc cctgcgtatg agtgcaagtg ggttttagga ccaggatgag       60

gcggggtggg ggtgcctacc tgacgaccga ccccgaccca ctggacaagc acccaacccc      120

cattccccaa attgcgcatc ccctatcaga gagggggagg ggaaacagga tgcggcgagg      180

cgcgtgcgca ctgccagctt cagcaccgcg gacagtgcct tcgcccccgc ctggcggcgc      240

gcgccaccgc cgcctcagca ctgaaggcgc gctgacgtca ctcgccggtc ccccgcaaac      300

tccccttccc ggccaccttg gtcgcgtccg cgccgccgcc ggcccagccg gaccgcacca      360

cgcgaggcgc gagatagggg ggcacgggcg cgaccatctg cgctgcggcg ccggcgactc      420

agcgctgcct cagtctgcgg tgggcagcgg aggagtcgtg tcgtgcctga gagcgcagtc      480

gagaa                                                                  485


<210>  54
<211>  229
<212>  DNA
<213>  Mus

<400>  54
agctgaatgg ggtccgcctc ttttccctgc ctaaacagac aggaactcct gccaattgag       60

ggcgtcaccg ctaaggctcc gccccagcct gggctccaca accaatgaag ggtaatctcg      120

acaaagagca aggggtgggg cgcgggcgcg caggtgcagc agcacacagg ctggtcggga      180

gggcggggcg cgacgtctgc cgtgcggggt cccggcatcg gttgcgcgc                  229


<210>  55
<211>  30
<212>  DNA
<213>  Unknown

<220>
<223>  HA: HA-Tag

<400>  55
atgtacccat acgatgttcc agattacgct                                        30


<210>  56
<211>  33
<212>  DNA
<213>  Unknown

<220>
<223>  NLS

<400>  56
tcgccgaaga aaaagcgcaa ggtcgaagcg tcc                                    33


<210>  57
<211>  48
<212>  DNA
<213>  Unknown

<220>
<223>  spA: Short poly A signal

<400>  57
aataaaagat ctttattttc attagatctg tgtgttggtt ttttgtgt                    48


<210>  58
<211>  72
<212>  DNA
<213>  Unknown

<220>
<223>  tRNAp:

<400>  58
ggctcgttgg tctaggggta tgattctcgc ttagggtgcg agaggtcccg ggttcaaatc       60

ccggacgagc cc                                                           72


<210>  59
<211>  249
<212>  DNA
<213>  Unknown

<220>
<223>  pU6

<400>  59
gagggcctat ttcccatgat tccttcatat ttgcatatac gatacaaggc tgttagagag       60

ataattggaa ttaatttgac tgtaaacaca aagatattag tacaaaatac gtgacgtaga      120

aagtaataat ttcttgggta gtttgcagtt ttaaaattat gttttaaaat ggactatcat      180

atgcttaccg taacttgaaa gtatttcgat ttcttggctt tatatatctt gtggaaagga      240

cgaaacacc                                                              249


<210>  60
<211>  49
<212>  DNA
<213>  Unknown

<220>
<223>  U6 promoter

<400>  60
gtaatttcta ctgttgtaga tggaagagca tatatgctct tcttttttt                   49


<210>  61
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  DNMT1 23nt guide

<400>  61
ctgatggtcc atgtctgtta ctc                                               23


<210>  62
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  EMX1 23nt guide

<400>  62
tggttgccca ccctagtcat tgg                                               23


<210>  63
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  VEGFA 23nt guide

<400>  63
ctaggaatat tgaagggggc agg                                               23


<210>  64
<211>  23
<212>  DNA
<213>  Artificial sequence

<220>
<223>  GRIN2b 23nt guide

<400>  64
gtgctcaatg aaaggagata agg                                               23


<210>  65
<211>  57
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  array 1 T1

<400>  65
agatctgatg gtccatgtct gttactcaat ttctactctt gtagattggt tgcccac          57


<210>  66
<211>  96
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  array 1 T2

<400>  66
cctagtcatt ggaatttcta ctcttgtaga tctaggaata ttgaaggggg caggaatttc       60

tactcttgta gatgtgctca atgaaaggag ataagg                                 96


<210>  67
<211>  57
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  array 1 B1

<400>  67
aaaaccttat ctcctttcat tgagcacatc tacaagagta gaaattcctg ccccctt          57


<210>  68
<211>  96
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  array 1 B2

<400>  68
caatattcct agatctacaa gagtagaaat tccaatgact agggtgggca accaatctac       60

aagagtagaa attgagtaac agacatggac catcag                                 96


<210>  69
<211>  71
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  array 2 T1

<400>  69
agatctgatg gtccatgtct gttactcgcc tgtcaatttc tactcttgta gattggttgc       60

ccaccctagt c                                                            71


<210>  70
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic

<400>  70
gagtctaagc agaagaagaa                                                   20


<210>  71
<211>  5
<212>  PRT
<213>  Unknown

<220>
<223>  Unknown


<220>
<221>  MOD_RES
<222>  (5)..(5)
<223>  Glycine at position 5 is pegylated

<400>  71

Ala Pro Arg Pro Gly 
1               5   


<210>  72
<211>  5
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic

<400>  72

His Lys Arg Glu Asp 
1               5   


