                         SEQUENCE LISTING

<110>  Navigo Proteins GmbH
 
<120>  ALKALINE STABLE FC-BINDING PROTEINS FOR AFFINITY CHROMATOGRAPHY

<130>  SP32-3 WO R

<160>  20    

<170>  PatentIn version 3.5

<210>  1
<211>  58
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  cs24

<400>  1

Ile Ala Ala Gln His Asp Lys Glu Gln Gln Ala Ala Phe Tyr Glu Ile 
1               5                   10                  15      


Leu His Leu Pro Asn Leu Thr Glu Asp Gln Arg Asn Ala Phe Ile Gln 
            20                  25                  30          


Ser Leu Arg Asp Asp Pro Ser Val Ser Leu Glu Ile Leu Gly Glu Ala 
        35                  40                  45              


Lys Lys Leu Asn Asp Ala Gln Ala Pro Lys 
    50                  55              


<210>  2
<211>  58
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  cs26

<400>  2

Ile Ala Ala Gln His Asp Lys Asp Gln Gln Ala Ala Phe Tyr Glu Ile 
1               5                   10                  15      


Leu His Leu Pro Asn Leu Thr Glu Glu Gln Arg Asn Ala Phe Ile Gln 
            20                  25                  30          


Ser Leu Arg Asp Asp Pro Ser Val Ser Leu Glu Ile Leu Ala Glu Ala 
        35                  40                  45              


Lys Lys Leu Asn Asp Ala Gln Ala Pro Lys 
    50                  55              


<210>  3
<211>  58
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  cs24a

<400>  3

Ile Ala Ala Gln His Asp Lys Glu His Gln Ala Ala Phe Tyr Glu Ile 
1               5                   10                  15      


Leu His Leu Pro Asn Leu Thr Glu Asp Gln Arg Asn Ala Phe Ile Gln 
            20                  25                  30          


Ser Leu Arg Asp Asp Pro Ser Val Ser Leu Glu Ile Leu Gly Glu Ala 
        35                  40                  45              


Lys Lys Leu Asn Asp Ala Gln Ala Pro Lys 
    50                  55              


<210>  4
<211>  58
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  cs26a

<400>  4

Ile Ala Ala Gln His Asp Lys Asp His Gln Ala Ala Phe Tyr Glu Ile 
1               5                   10                  15      


Leu His Leu Pro Asn Leu Thr Glu Glu Gln Arg Asn Ala Phe Ile Gln 
            20                  25                  30          


Ser Leu Arg Asp Asp Pro Ser Val Ser Leu Glu Ile Leu Ala Glu Ala 
        35                  40                  45              


Lys Lys Leu Asn Asp Ala Gln Ala Pro Lys 
    50                  55              


<210>  5
<211>  58
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  cs24b

<400>  5

Ile Ala Ala Gln His Asp Lys Glu Gln Gln Ala Ala Phe Tyr Glu Ile 
1               5                   10                  15      


Leu His Leu Pro Asn Leu Thr Glu Asp Gln Arg Asn Ala Phe Ile Gln 
            20                  25                  30          


Ser Leu Arg His Asp Pro Ser Val Ser Leu Glu Ile Leu Gly Glu Ala 
        35                  40                  45              


Lys Lys Leu Asn Asp Ala Gln Ala Pro Lys 
    50                  55              


<210>  6
<211>  58
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  cs26b

<400>  6

Ile Ala Ala Gln His Asp Lys Asp Gln Gln Ala Ala Phe Tyr Glu Ile 
1               5                   10                  15      


Leu His Leu Pro Asn Leu Thr Glu Glu Gln Arg Asn Ala Phe Ile Gln 
            20                  25                  30          


Ser Leu Arg His Asp Pro Ser Val Ser Leu Glu Ile Leu Ala Glu Ala 
        35                  40                  45              


Lys Lys Leu Asn Asp Ala Gln Ala Pro Lys 
    50                  55              


<210>  7
<211>  53
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  cs24 delNC

<400>  7

Gln His Asp Lys Glu Gln Gln Ala Ala Phe Tyr Glu Ile Leu His Leu 
1               5                   10                  15      


Pro Asn Leu Thr Glu Asp Gln Arg Asn Ala Phe Ile Gln Ser Leu Arg 
            20                  25                  30          


Asp Asp Pro Ser Val Ser Leu Glu Ile Leu Gly Glu Ala Lys Lys Leu 
        35                  40                  45              


Asn Asp Ala Gln Ala 
    50              


<210>  8
<211>  53
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  cs26 delNC

<400>  8

Gln His Asp Lys Asp Gln Gln Ala Ala Phe Tyr Glu Ile Leu His Leu 
1               5                   10                  15      


Pro Asn Leu Thr Glu Glu Gln Arg Asn Ala Phe Ile Gln Ser Leu Arg 
            20                  25                  30          


Asp Asp Pro Ser Val Ser Leu Glu Ile Leu Ala Glu Ala Lys Lys Leu 
        35                  40                  45              


Asn Asp Ala Gln Ala 
    50              


<210>  9
<211>  55
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  cs24 delN

<400>  9

Ala His Asp Lys Glu Gln Gln Ala Ala Phe Tyr Glu Ile Leu His Leu 
1               5                   10                  15      


Pro Asn Leu Thr Glu Asp Gln Arg Asn Ala Phe Ile Gln Ser Leu Arg 
            20                  25                  30          


Asp Asp Pro Ser Val Ser Leu Glu Ile Leu Gly Glu Ala Lys Lys Leu 
        35                  40                  45              


Asn Asp Ala Gln Ala Pro Lys 
    50                  55  


<210>  10
<211>  55
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  cs26 delN

<400>  10

Ala His Asp Lys Asp Gln Gln Ala Ala Phe Tyr Glu Ile Leu His Leu 
1               5                   10                  15      


Pro Asn Leu Thr Glu Glu Gln Arg Asn Ala Phe Ile Gln Ser Leu Arg 
            20                  25                  30          


Asp Asp Pro Ser Val Ser Leu Glu Ile Leu Ala Glu Ala Lys Lys Leu 
        35                  40                  45              


Asn Asp Ala Gln Ala Pro Lys 
    50                  55  


<210>  11
<211>  318
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  cs24 delNC hexamer

<400>  11

Gln His Asp Lys Glu Gln Gln Ala Ala Phe Tyr Glu Ile Leu His Leu 
1               5                   10                  15      


Pro Asn Leu Thr Glu Asp Gln Arg Asn Ala Phe Ile Gln Ser Leu Arg 
            20                  25                  30          


Asp Asp Pro Ser Val Ser Leu Glu Ile Leu Gly Glu Ala Lys Lys Leu 
        35                  40                  45              


Asn Asp Ala Gln Ala Gln His Asp Lys Glu Gln Gln Ala Ala Phe Tyr 
    50                  55                  60                  


Glu Ile Leu His Leu Pro Asn Leu Thr Glu Asp Gln Arg Asn Ala Phe 
65                  70                  75                  80  


Ile Gln Ser Leu Arg Asp Asp Pro Ser Val Ser Leu Glu Ile Leu Gly 
                85                  90                  95      


Glu Ala Lys Lys Leu Asn Asp Ala Gln Ala Gln His Asp Lys Glu Gln 
            100                 105                 110         


Gln Ala Ala Phe Tyr Glu Ile Leu His Leu Pro Asn Leu Thr Glu Asp 
        115                 120                 125             


Gln Arg Asn Ala Phe Ile Gln Ser Leu Arg Asp Asp Pro Ser Val Ser 
    130                 135                 140                 


Leu Glu Ile Leu Gly Glu Ala Lys Lys Leu Asn Asp Ala Gln Ala Gln 
145                 150                 155                 160 


His Asp Lys Glu Gln Gln Ala Ala Phe Tyr Glu Ile Leu His Leu Pro 
                165                 170                 175     


Asn Leu Thr Glu Asp Gln Arg Asn Ala Phe Ile Gln Ser Leu Arg Asp 
            180                 185                 190         


Asp Pro Ser Val Ser Leu Glu Ile Leu Gly Glu Ala Lys Lys Leu Asn 
        195                 200                 205             


Asp Ala Gln Ala Gln His Asp Lys Glu Gln Gln Ala Ala Phe Tyr Glu 
    210                 215                 220                 


Ile Leu His Leu Pro Asn Leu Thr Glu Asp Gln Arg Asn Ala Phe Ile 
225                 230                 235                 240 


Gln Ser Leu Arg Asp Asp Pro Ser Val Ser Leu Glu Ile Leu Gly Glu 
                245                 250                 255     


Ala Lys Lys Leu Asn Asp Ala Gln Ala Gln His Asp Lys Glu Gln Gln 
            260                 265                 270         


Ala Ala Phe Tyr Glu Ile Leu His Leu Pro Asn Leu Thr Glu Asp Gln 
        275                 280                 285             


Arg Asn Ala Phe Ile Gln Ser Leu Arg Asp Asp Pro Ser Val Ser Leu 
    290                 295                 300                 


Glu Ile Leu Gly Glu Ala Lys Lys Leu Asn Asp Ala Gln Ala 
305                 310                 315             


<210>  12
<211>  318
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  cs26 delNC hexamer

<400>  12

Gln His Asp Lys Asp Gln Gln Ala Ala Phe Tyr Glu Ile Leu His Leu 
1               5                   10                  15      


Pro Asn Leu Thr Glu Glu Gln Arg Asn Ala Phe Ile Gln Ser Leu Arg 
            20                  25                  30          


Asp Asp Pro Ser Val Ser Leu Glu Ile Leu Ala Glu Ala Lys Lys Leu 
        35                  40                  45              


Asn Asp Ala Gln Ala Gln His Asp Lys Asp Gln Gln Ala Ala Phe Tyr 
    50                  55                  60                  


Glu Ile Leu His Leu Pro Asn Leu Thr Glu Glu Gln Arg Asn Ala Phe 
65                  70                  75                  80  


Ile Gln Ser Leu Arg Asp Asp Pro Ser Val Ser Leu Glu Ile Leu Ala 
                85                  90                  95      


Glu Ala Lys Lys Leu Asn Asp Ala Gln Ala Gln His Asp Lys Asp Gln 
            100                 105                 110         


Gln Ala Ala Phe Tyr Glu Ile Leu His Leu Pro Asn Leu Thr Glu Glu 
        115                 120                 125             


Gln Arg Asn Ala Phe Ile Gln Ser Leu Arg Asp Asp Pro Ser Val Ser 
    130                 135                 140                 


Leu Glu Ile Leu Ala Glu Ala Lys Lys Leu Asn Asp Ala Gln Ala Gln 
145                 150                 155                 160 


His Asp Lys Asp Gln Gln Ala Ala Phe Tyr Glu Ile Leu His Leu Pro 
                165                 170                 175     


Asn Leu Thr Glu Glu Gln Arg Asn Ala Phe Ile Gln Ser Leu Arg Asp 
            180                 185                 190         


Asp Pro Ser Val Ser Leu Glu Ile Leu Ala Glu Ala Lys Lys Leu Asn 
        195                 200                 205             


Asp Ala Gln Ala Gln His Asp Lys Asp Gln Gln Ala Ala Phe Tyr Glu 
    210                 215                 220                 


Ile Leu His Leu Pro Asn Leu Thr Glu Glu Gln Arg Asn Ala Phe Ile 
225                 230                 235                 240 


Gln Ser Leu Arg Asp Asp Pro Ser Val Ser Leu Glu Ile Leu Ala Glu 
                245                 250                 255     


Ala Lys Lys Leu Asn Asp Ala Gln Ala Gln His Asp Lys Asp Gln Gln 
            260                 265                 270         


Ala Ala Phe Tyr Glu Ile Leu His Leu Pro Asn Leu Thr Glu Glu Gln 
        275                 280                 285             


Arg Asn Ala Phe Ile Gln Ser Leu Arg Asp Asp Pro Ser Val Ser Leu 
    290                 295                 300                 


Glu Ile Leu Ala Glu Ala Lys Lys Leu Asn Asp Ala Gln Ala 
305                 310                 315             


<210>  13
<211>  290
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  cs24 pentamer

<400>  13

Ile Ala Ala Gln His Asp Lys Glu Gln Gln Ala Ala Phe Tyr Glu Ile 
1               5                   10                  15      


Leu His Leu Pro Asn Leu Thr Glu Asp Gln Arg Asn Ala Phe Ile Gln 
            20                  25                  30          


Ser Leu Arg Asp Asp Pro Ser Val Ser Leu Glu Ile Leu Gly Glu Ala 
        35                  40                  45              


Lys Lys Leu Asn Asp Ala Gln Ala Pro Lys Ile Ala Ala Gln His Asp 
    50                  55                  60                  


Lys Glu Gln Gln Ala Ala Phe Tyr Glu Ile Leu His Leu Pro Asn Leu 
65                  70                  75                  80  


Thr Glu Asp Gln Arg Asn Ala Phe Ile Gln Ser Leu Arg Asp Asp Pro 
                85                  90                  95      


Ser Val Ser Leu Glu Ile Leu Gly Glu Ala Lys Lys Leu Asn Asp Ala 
            100                 105                 110         


Gln Ala Pro Lys Ile Ala Ala Gln His Asp Lys Glu Gln Gln Ala Ala 
        115                 120                 125             


Phe Tyr Glu Ile Leu His Leu Pro Asn Leu Thr Glu Asp Gln Arg Asn 
    130                 135                 140                 


Ala Phe Ile Gln Ser Leu Arg Asp Asp Pro Ser Val Ser Leu Glu Ile 
145                 150                 155                 160 


Leu Gly Glu Ala Lys Lys Leu Asn Asp Ala Gln Ala Pro Lys Ile Ala 
                165                 170                 175     


Ala Gln His Asp Lys Glu Gln Gln Ala Ala Phe Tyr Glu Ile Leu His 
            180                 185                 190         


Leu Pro Asn Leu Thr Glu Asp Gln Arg Asn Ala Phe Ile Gln Ser Leu 
        195                 200                 205             


Arg Asp Asp Pro Ser Val Ser Leu Glu Ile Leu Gly Glu Ala Lys Lys 
    210                 215                 220                 


Leu Asn Asp Ala Gln Ala Pro Lys Ile Ala Ala Gln His Asp Lys Glu 
225                 230                 235                 240 


Gln Gln Ala Ala Phe Tyr Glu Ile Leu His Leu Pro Asn Leu Thr Glu 
                245                 250                 255     


Asp Gln Arg Asn Ala Phe Ile Gln Ser Leu Arg Asp Asp Pro Ser Val 
            260                 265                 270         


Ser Leu Glu Ile Leu Gly Glu Ala Lys Lys Leu Asn Asp Ala Gln Ala 
        275                 280                 285             


Pro Lys 
    290 


<210>  14
<211>  290
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  cs26 pentamer

<400>  14

Ile Ala Ala Gln His Asp Lys Asp Gln Gln Ala Ala Phe Tyr Glu Ile 
1               5                   10                  15      


Leu His Leu Pro Asn Leu Thr Glu Glu Gln Arg Asn Ala Phe Ile Gln 
            20                  25                  30          


Ser Leu Arg Asp Asp Pro Ser Val Ser Leu Glu Ile Leu Ala Glu Ala 
        35                  40                  45              


Lys Lys Leu Asn Asp Ala Gln Ala Pro Lys Ile Ala Ala Gln His Asp 
    50                  55                  60                  


Lys Asp Gln Gln Ala Ala Phe Tyr Glu Ile Leu His Leu Pro Asn Leu 
65                  70                  75                  80  


Thr Glu Glu Gln Arg Asn Ala Phe Ile Gln Ser Leu Arg Asp Asp Pro 
                85                  90                  95      


Ser Val Ser Leu Glu Ile Leu Ala Glu Ala Lys Lys Leu Asn Asp Ala 
            100                 105                 110         


Gln Ala Pro Lys Ile Ala Ala Gln His Asp Lys Asp Gln Gln Ala Ala 
        115                 120                 125             


Phe Tyr Glu Ile Leu His Leu Pro Asn Leu Thr Glu Glu Gln Arg Asn 
    130                 135                 140                 


Ala Phe Ile Gln Ser Leu Arg Asp Asp Pro Ser Val Ser Leu Glu Ile 
145                 150                 155                 160 


Leu Ala Glu Ala Lys Lys Leu Asn Asp Ala Gln Ala Pro Lys Ile Ala 
                165                 170                 175     


Ala Gln His Asp Lys Asp Gln Gln Ala Ala Phe Tyr Glu Ile Leu His 
            180                 185                 190         


Leu Pro Asn Leu Thr Glu Glu Gln Arg Asn Ala Phe Ile Gln Ser Leu 
        195                 200                 205             


Arg Asp Asp Pro Ser Val Ser Leu Glu Ile Leu Ala Glu Ala Lys Lys 
    210                 215                 220                 


Leu Asn Asp Ala Gln Ala Pro Lys Ile Ala Ala Gln His Asp Lys Asp 
225                 230                 235                 240 


Gln Gln Ala Ala Phe Tyr Glu Ile Leu His Leu Pro Asn Leu Thr Glu 
                245                 250                 255     


Glu Gln Arg Asn Ala Phe Ile Gln Ser Leu Arg Asp Asp Pro Ser Val 
            260                 265                 270         


Ser Leu Glu Ile Leu Ala Glu Ala Lys Lys Leu Asn Asp Ala Gln Ala 
        275                 280                 285             


Pro Lys 
    290 


<210>  15
<211>  174
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  cs24 tetramer

<400>  15

Ile Ala Ala Gln His Asp Lys Glu Gln Gln Ala Ala Phe Tyr Glu Ile 
1               5                   10                  15      


Leu His Leu Pro Asn Leu Thr Glu Asp Gln Arg Asn Ala Phe Ile Gln 
            20                  25                  30          


Ser Leu Arg Asp Asp Pro Ser Val Ser Leu Glu Ile Leu Gly Glu Ala 
        35                  40                  45              


Lys Lys Leu Asn Asp Ala Gln Ala Pro Lys Ile Ala Ala Gln His Asp 
    50                  55                  60                  


Lys Glu Gln Gln Ala Ala Phe Tyr Glu Ile Leu His Leu Pro Asn Leu 
65                  70                  75                  80  


Thr Glu Asp Gln Arg Asn Ala Phe Ile Gln Ser Leu Arg Asp Asp Pro 
                85                  90                  95      


Ser Val Ser Leu Glu Ile Leu Gly Glu Ala Lys Lys Leu Asn Asp Ala 
            100                 105                 110         


Gln Ala Pro Lys Ile Ala Ala Gln His Asp Lys Glu Gln Gln Ala Ala 
        115                 120                 125             


Phe Tyr Glu Ile Leu His Leu Pro Asn Leu Thr Glu Asp Gln Arg Asn 
    130                 135                 140                 


Ala Phe Ile Gln Ser Leu Arg Asp Asp Pro Ser Val Ser Leu Glu Ile 
145                 150                 155                 160 


Leu Gly Glu Ala Lys Lys Leu Asn Asp Ala Gln Ala Pro Lys 
                165                 170                 


<210>  16
<211>  174
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  cs26 tetramer

<400>  16

Ile Ala Ala Gln His Asp Lys Asp Gln Gln Ala Ala Phe Tyr Glu Ile 
1               5                   10                  15      


Leu His Leu Pro Asn Leu Thr Glu Glu Gln Arg Asn Ala Phe Ile Gln 
            20                  25                  30          


Ser Leu Arg Asp Asp Pro Ser Val Ser Leu Glu Ile Leu Ala Glu Ala 
        35                  40                  45              


Lys Lys Leu Asn Asp Ala Gln Ala Pro Lys Ile Ala Ala Gln His Asp 
    50                  55                  60                  


Lys Asp Gln Gln Ala Ala Phe Tyr Glu Ile Leu His Leu Pro Asn Leu 
65                  70                  75                  80  


Thr Glu Glu Gln Arg Asn Ala Phe Ile Gln Ser Leu Arg Asp Asp Pro 
                85                  90                  95      


Ser Val Ser Leu Glu Ile Leu Ala Glu Ala Lys Lys Leu Asn Asp Ala 
            100                 105                 110         


Gln Ala Pro Lys Ile Ala Ala Gln His Asp Lys Asp Gln Gln Ala Ala 
        115                 120                 125             


Phe Tyr Glu Ile Leu His Leu Pro Asn Leu Thr Glu Glu Gln Arg Asn 
    130                 135                 140                 


Ala Phe Ile Gln Ser Leu Arg Asp Asp Pro Ser Val Ser Leu Glu Ile 
145                 150                 155                 160 


Leu Ala Glu Ala Lys Lys Leu Asn Asp Ala Gln Ala Pro Lys 
                165                 170                 


<210>  17
<211>  58
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  IB24 parental

<400>  17

Ala Ala Ala Gln His Asp Lys Glu Gln Gln Ser Ala Phe Tyr Glu Ile 
1               5                   10                  15      


Leu His Leu Pro Asn Leu Thr Glu Asp Gln Arg Asn Ala Phe Ile Gln 
            20                  25                  30          


Ser Leu Lys Asp Asp Pro Ser Val Ser Lys Glu Ile Leu Gly Glu Ala 
        35                  40                  45              


Lys Lys Leu Asn Asp Ala Gln Ala Pro Lys 
    50                  55              


<210>  18
<211>  58
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  IB26 parental

<400>  18

Ala Ala Ala Gln His Asp Lys Asp Gln Gln Ser Ala Phe Tyr Glu Ile 
1               5                   10                  15      


Leu His Leu Pro Asn Leu Thr Glu Glu Gln Arg Asn Ala Phe Ile Gln 
            20                  25                  30          


Ser Leu Lys Asp Asp Pro Ser Val Ser Lys Glu Ile Leu Ala Glu Ala 
        35                  40                  45              


Lys Lys Leu Asn Asp Ala Gln Ala Pro Lys 
    50                  55              


<210>  19
<211>  13
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  coupling sequence

<400>  19

Ala Ser Pro Ala Pro Ser Ala Pro Ser Ala Cys Ala Ser 
1               5                   10              


<210>  20
<211>  8
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  streptaq

<400>  20

Trp Ser His Pro Gln Phe Glu Lys 
1               5               


<210>  21
<211>  58
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  cs26c

<400>  2

Ile Ala Ala Gln His Asp Lys Asp Gln Gln Ser Ala Phe Tyr Glu Ile 
1               5                   10                  15      


Leu His Leu Pro Asn Leu Thr Glu Glu Gln Arg Asn Ala Phe Ile Gln 
            20                  25                  30          


Ser Leu Arg Asp Asp Pro Ser Val Ser Leu Glu Ile Leu Ala Glu Ala 
        35                  40                  45              


Lys Lys Leu Asn Asp Ala Gln Ala Pro Lys 
    50                  55              