
                         SEQUENCE LISTING

<110>  9636137 Canada Inc.
 
<120>  CORTICAL NEURON CELL MODEL OF ALZHEIMER'S DISEASE BASED ON BMI1 
       DEFICIENCY, AND USES THEREOF

<130>  306836.7

<150>  US 62/369,814
<151>  2016-08-02

<160>  6     

<170>  PatentIn version 3.5

<210>  1
<211>  3435
<212>  DNA
<213>  Homo sapiens


<220>
<221>  CDS
<222>  (507)..(1487)

<400>  1
acagcaacta tgaaataatc gtagtatgag aggcagagat cggggcgaga caatggggat       60

gtgggcgcgg gagccccgtt ccggcttagc agcacctccc agccccgcag aataaaaccg      120

atcgcgcccc ctccgcgcgc gccctccccc gagtgcggag cgggaggagg cggcggcggc      180

cgaggaggag gaggaggagg ccccggagga ggaggcgttg gaggtcgagg cggaggcgga      240

ggaggaggag gccgaggcgc cggaggaggc cgaggcgccg gagcaggagg aggccggccg      300

gaggcggcat gagacgagcg tggcggccgc ggctgctcgg ggccgcgctg gttgcccatt      360

gacagcggcg tctgcagctc gcttcaagat ggccgcttgg ctcgcattca ttttctgctg      420

aacgactttt aactttcatt gtcttttccg cccgcttcga tcgcctcgcg ccggctgctc      480

tttccgggat tttttatcaa gcagaa atg cat cga aca acg aga atc aag atc       533
                             Met His Arg Thr Thr Arg Ile Lys Ile          
                             1               5                            

act gag cta aat ccc cac ctg atg tgt gtg ctt tgt gga ggg tac ttc        581
Thr Glu Leu Asn Pro His Leu Met Cys Val Leu Cys Gly Gly Tyr Phe           
10                  15                  20                  25            

att gat gcc aca acc ata ata gaa tgt cta cat tcc ttc tgt aaa acg        629
Ile Asp Ala Thr Thr Ile Ile Glu Cys Leu His Ser Phe Cys Lys Thr           
                30                  35                  40                

tgt att gtt cgt tac ctg gag acc agc aag tat tgt cct att tgt gat        677
Cys Ile Val Arg Tyr Leu Glu Thr Ser Lys Tyr Cys Pro Ile Cys Asp           
            45                  50                  55                    

gtc caa gtt cac aag acc aga cca cta ctg aat ata agg tca gat aaa        725
Val Gln Val His Lys Thr Arg Pro Leu Leu Asn Ile Arg Ser Asp Lys           
        60                  65                  70                        

act ctc caa gat att gta tac aaa tta gtt cca ggg ctt ttc aaa aat        773
Thr Leu Gln Asp Ile Val Tyr Lys Leu Val Pro Gly Leu Phe Lys Asn           
    75                  80                  85                            

gaa atg aag aga aga agg gat ttt tat gca gct cat cct tct gct gat        821
Glu Met Lys Arg Arg Arg Asp Phe Tyr Ala Ala His Pro Ser Ala Asp           
90                  95                  100                 105           

gct gcc aat ggc tct aat gaa gat aga gga gag gtt gca gat gaa gat        869
Ala Ala Asn Gly Ser Asn Glu Asp Arg Gly Glu Val Ala Asp Glu Asp           
                110                 115                 120               

aag aga att ata act gat gat gag ata ata agc tta tcc att gaa ttc        917
Lys Arg Ile Ile Thr Asp Asp Glu Ile Ile Ser Leu Ser Ile Glu Phe           
            125                 130                 135                   

ttt gac cag aac aga ttg gat cgg aaa gta aac aaa gac aaa gag aaa        965
Phe Asp Gln Asn Arg Leu Asp Arg Lys Val Asn Lys Asp Lys Glu Lys           
        140                 145                 150                       

tct aag gag gag gtg aat gat aaa aga tac tta cga tgc cca gca gca       1013
Ser Lys Glu Glu Val Asn Asp Lys Arg Tyr Leu Arg Cys Pro Ala Ala           
    155                 160                 165                           

atg act gtg atg cac tta aga aag ttt ctc aga agt aaa atg gac ata       1061
Met Thr Val Met His Leu Arg Lys Phe Leu Arg Ser Lys Met Asp Ile           
170                 175                 180                 185           

cct aat act ttc cag att gat gtc atg tat gag gag gaa cct tta aag       1109
Pro Asn Thr Phe Gln Ile Asp Val Met Tyr Glu Glu Glu Pro Leu Lys           
                190                 195                 200               

gat tat tat aca cta atg gat att gcc tac att tat acc tgg aga agg       1157
Asp Tyr Tyr Thr Leu Met Asp Ile Ala Tyr Ile Tyr Thr Trp Arg Arg           
            205                 210                 215                   

aat ggt cca ctt cca ttg aaa tac aga gtt cga cct act tgt aaa aga       1205
Asn Gly Pro Leu Pro Leu Lys Tyr Arg Val Arg Pro Thr Cys Lys Arg           
        220                 225                 230                       

atg aag atc agt cac cag aga gat gga ctg aca aat gct gga gaa ctg       1253
Met Lys Ile Ser His Gln Arg Asp Gly Leu Thr Asn Ala Gly Glu Leu           
    235                 240                 245                           

gaa agt gac tct ggg agt gac aag gcc aac agc cca gca gga ggt att       1301
Glu Ser Asp Ser Gly Ser Asp Lys Ala Asn Ser Pro Ala Gly Gly Ile           
250                 255                 260                 265           

ccc tcc acc tct tct tgt ttg cct agc ccc agt act cca gtg cag tct       1349
Pro Ser Thr Ser Ser Cys Leu Pro Ser Pro Ser Thr Pro Val Gln Ser           
                270                 275                 280               

cct cat cca cag ttt cct cac att tcc agt act atg aat gga acc agc       1397
Pro His Pro Gln Phe Pro His Ile Ser Ser Thr Met Asn Gly Thr Ser           
            285                 290                 295                   

aac agc ccc agc ggt aac cac caa tct tct ttt gcc aat aga cct cga       1445
Asn Ser Pro Ser Gly Asn His Gln Ser Ser Phe Ala Asn Arg Pro Arg           
        300                 305                 310                       

aaa tca tca gta aat ggg tca tca gca act tct tct ggt tga               1487
Lys Ser Ser Val Asn Gly Ser Ser Ala Thr Ser Ser Gly                       
    315                 320                 325                           

tacctgagac tgttaaggaa aaaaatttta aacccctgat ttatatagat atcttcatgc     1547

cattacagct ttctagatgc taatacatgt gactatcgtc caatttgctt tcttttgtag     1607

tgacattaaa tttggctata aaagatggac tacatgtgat actcctatgg acgttaattg     1667

aaaagaaaga ttgttgttat aaagaattgg tttcttggaa agcaggcaag actttttctc     1727

tgtgttagga aagatgggaa atggtttctg taaccattgt ttggatttgg aagtactctg     1787

cagtggacat aagcattggg ccatagtttg ttaatctcaa ctaacgccta cattacattc     1847

tccttgatcg ttcttgttat tacgctgttt tgtgaacctg tagaaaacaa gtgcttttta     1907

tcttgaaatt caaccaacgg aaagaatatg catagaataa tgcattctat gtagccatgt     1967

cactgtgaat aacgatttct tgcatattta gccattttga ttcctgtttg atttatactt     2027

ctctgttgct acgcaaaacc gatcaaagaa aagtgaactt cagttttaca atctgtatgc     2087

ctaaaagcgg gtactaccgt ttattttact gacttgttta aatgattcgc ttttgtaaga     2147

atcagatggc attatgcttg ttgtacaatg ccatattggt atatgacata acaggaaaca     2207

gtattgtatg atatatttat aaatgctata aagaaatatt gtgtttcatg cattcagaaa     2267

tgattgttaa aattctccca actggttcga cctttgcaga tacccataac ctatgttgag     2327

ccttgcttac cagcaaagaa tatttttaat gtggatatct aattctaaag tctgttccat     2387

tagaagcaat tggcacatct ttctatactt tatatacttt tctccagtaa tacatgttta     2447

ctttaaagat tgttgcagtg aagaaaaacc tttaactgag aaatatggaa accgtcttaa     2507

ttttccattg gctatgatgg aattaatatt gtattttaaa aatgcatatt gatcactata     2567

attctaaaac aattttttaa ataaaccagc aggttgctaa aagaaggcat tttatctaaa     2627

gttattttaa taggtggtat agcagtaatt ttaaatttaa gagttgcttt tacagttaac     2687

aatggaatat gccttctctg ctatgtctga aaatagaagc tatttattat gagcttctac     2747

aggtattttt aaatagagca agcatgttga atttaaaata tgaataaccc cacccaacaa     2807

ttttcagttt attttttgct ttggtcgaac ttggtgtgtg ttcatcaccc atcagttatt     2867

tgtgagggtg tttattctat atgaatattg tttcatgttt gtatgggaaa attgtagcta     2927

aacatttcat tgtccccagt ctgcaaaaga agcacaattc tattgctttg tcttgcttat     2987

agtcattaaa tcattacttt tacatatatt gctgttactt ctgctttctt taaaaatata     3047

gtaaaggatg ttttatgaag tcacaagata catatatttt tattttgacc taaatttgta     3107

cagtcccatt gtaagtgttg tttctaatta tagatgtaaa atgaaatttc atttgtaatt     3167

ggaaaaaatc caataaaaag gatattcatt tagaaaatag ctaagatctt taataaaaat     3227

ttgatatgaa aagcacaatg tgcagaagtt atggaaaacc tatagaggat tacaacaggt     3287

aaacgttaaa gagaatacat tgctgactta tagtgatgtg gctaagaagt acatgctttg     3347

ttgtaaaatt gcttgaaagc ccattgaaag atgtatctgt ttatttacag tctttgaagt     3407

aaaagttacc aatgtttgcc aataaaaa                                        3435


<210>  2
<211>  326
<212>  PRT
<213>  Homo sapiens

<400>  2

Met His Arg Thr Thr Arg Ile Lys Ile Thr Glu Leu Asn Pro His Leu 
1               5                   10                  15      


Met Cys Val Leu Cys Gly Gly Tyr Phe Ile Asp Ala Thr Thr Ile Ile 
            20                  25                  30          


Glu Cys Leu His Ser Phe Cys Lys Thr Cys Ile Val Arg Tyr Leu Glu 
        35                  40                  45              


Thr Ser Lys Tyr Cys Pro Ile Cys Asp Val Gln Val His Lys Thr Arg 
    50                  55                  60                  


Pro Leu Leu Asn Ile Arg Ser Asp Lys Thr Leu Gln Asp Ile Val Tyr 
65                  70                  75                  80  


Lys Leu Val Pro Gly Leu Phe Lys Asn Glu Met Lys Arg Arg Arg Asp 
                85                  90                  95      


Phe Tyr Ala Ala His Pro Ser Ala Asp Ala Ala Asn Gly Ser Asn Glu 
            100                 105                 110         


Asp Arg Gly Glu Val Ala Asp Glu Asp Lys Arg Ile Ile Thr Asp Asp 
        115                 120                 125             


Glu Ile Ile Ser Leu Ser Ile Glu Phe Phe Asp Gln Asn Arg Leu Asp 
    130                 135                 140                 


Arg Lys Val Asn Lys Asp Lys Glu Lys Ser Lys Glu Glu Val Asn Asp 
145                 150                 155                 160 


Lys Arg Tyr Leu Arg Cys Pro Ala Ala Met Thr Val Met His Leu Arg 
                165                 170                 175     


Lys Phe Leu Arg Ser Lys Met Asp Ile Pro Asn Thr Phe Gln Ile Asp 
            180                 185                 190         


Val Met Tyr Glu Glu Glu Pro Leu Lys Asp Tyr Tyr Thr Leu Met Asp 
        195                 200                 205             


Ile Ala Tyr Ile Tyr Thr Trp Arg Arg Asn Gly Pro Leu Pro Leu Lys 
    210                 215                 220                 


Tyr Arg Val Arg Pro Thr Cys Lys Arg Met Lys Ile Ser His Gln Arg 
225                 230                 235                 240 


Asp Gly Leu Thr Asn Ala Gly Glu Leu Glu Ser Asp Ser Gly Ser Asp 
                245                 250                 255     


Lys Ala Asn Ser Pro Ala Gly Gly Ile Pro Ser Thr Ser Ser Cys Leu 
            260                 265                 270         


Pro Ser Pro Ser Thr Pro Val Gln Ser Pro His Pro Gln Phe Pro His 
        275                 280                 285             


Ile Ser Ser Thr Met Asn Gly Thr Ser Asn Ser Pro Ser Gly Asn His 
    290                 295                 300                 


Gln Ser Ser Phe Ala Asn Arg Pro Arg Lys Ser Ser Val Asn Gly Ser 
305                 310                 315                 320 


Ser Ala Thr Ser Ser Gly 
                325     


<210>  3
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Sequence is completely synthesized

<400>  3
cctaatactt tccagattga t                                                 21


<210>  4
<211>  19
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Sequence is completely synthesized

<400>  4
ggtacttcat tgatgccac                                                    19


<210>  5
<211>  9
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Sequence is completely synthesized

<400>  5
ttcaagaga                                                                9


<210>  6
<211>  19
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Sequence is completely synthesized

<400>  6
acgtgtattg ttcgttacc                                                    19


