                         SEQUENCE LISTING

<110>  Tessa Theraapeutics Pte. Ltd.
       Clegg, Richard I
 
<120>  Chimeric Antigen Receptor

<130>  RIC/FP7291040

<150>  US62/366729
<151>  2016-07-26

<150>  US62/366731
<151>  2016-07-26

<160>  126   

<170>  PatentIn version 3.5

<210>  1
<211>  115
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  GC33 heavy chain variable region sequence

<400>  1

Gln Val Gln Leu Gln Gln Ser Gly Ala Glu Leu Val Arg Pro Gly Ala 
1               5                   10                  15      


Ser Val Lys Leu Ser Cys Lys Ala Ser Gly Tyr Thr Phe Thr Asp Tyr 
            20                  25                  30          


Glu Met His Trp Val Lys Gln Thr Pro Val His Gly Leu Lys Trp Ile 
        35                  40                  45              


Gly Ala Leu Asp Pro Lys Thr Gly Asp Thr Ala Tyr Ser Gln Lys Phe 
    50                  55                  60                  


Lys Gly Lys Ala Thr Leu Thr Ala Asp Lys Ser Ser Ser Thr Ala Tyr 
65                  70                  75                  80  


Met Glu Leu Arg Ser Leu Thr Ser Glu Asp Ser Ala Val Tyr Tyr Cys 
                85                  90                  95      


Thr Arg Phe Tyr Ser Tyr Thr Tyr Trp Gly Gln Gly Thr Leu Val Thr 
            100                 105                 110         


Val Ser Ala 
        115 


<210>  2
<211>  5
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  GC33 HC-CDR1

<400>  2

Asp Tyr Glu Met His 
1               5   


<210>  3
<211>  17
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  GC33 HC-CDR2

<400>  3

Ala Leu Asp Pro Lys Thr Gly Asp Thr Ala Tyr Ser Gln Lys Phe Lys 
1               5                   10                  15      


Gly 
    


<210>  4
<211>  6
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  GC33 HC-CDR3

<400>  4

Phe Tyr Ser Tyr Thr Tyr 
1               5       


<210>  5
<211>  112
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  GC33 light chain variable region sequence

<400>  5

Asp Val Val Met Thr Gln Thr Pro Leu Ser Leu Pro Val Ser Leu Gly 
1               5                   10                  15      


Asp Gln Ala Ser Ile Ser Cys Arg Ser Ser Gln Ser Leu Val His Ser 
            20                  25                  30          


Asn Gly Asn Thr Tyr Leu His Trp Tyr Leu Gln Lys Pro Gly Gln Ser 
        35                  40                  45              


Pro Lys Leu Leu Ile Tyr Lys Val Ser Asn Arg Phe Ser Gly Val Pro 
    50                  55                  60                  


Asp Arg Phe Ser Gly Ser Gly Ser Gly Thr Asp Phe Thr Leu Lys Ile 
65                  70                  75                  80  


Ser Arg Val Glu Ala Glu Asp Leu Gly Val Tyr Phe Cys Ser Gln Asn 
                85                  90                  95      


Thr His Val Pro Pro Thr Phe Gly Ser Gly Thr Lys Leu Glu Ile Lys 
            100                 105                 110         


<210>  6
<211>  16
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  GC33 LC-CDR1

<400>  6

Arg Ser Ser Gln Ser Leu Val His Ser Asn Gly Asn Thr Tyr Leu His 
1               5                   10                  15      


<210>  7
<211>  7
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  GC33 LC-CDR2

<400>  7

Lys Val Ser Asn Arg Phe Ser 
1               5           


<210>  8
<211>  9
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  GC33 LC-CDR3

<400>  8

Ser Gln Asn Thr His Val Pro Pro Thr 
1               5                   


<210>  9
<211>  242
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  GC33 scFv

<400>  9

Gln Val Gln Leu Gln Gln Ser Gly Ala Glu Leu Val Arg Pro Gly Ala 
1               5                   10                  15      


Ser Val Lys Leu Ser Cys Lys Ala Ser Gly Tyr Thr Phe Thr Asp Tyr 
            20                  25                  30          


Glu Met His Trp Val Lys Gln Thr Pro Val His Gly Leu Lys Trp Ile 
        35                  40                  45              


Gly Ala Leu Asp Pro Lys Thr Gly Asp Thr Ala Tyr Ser Gln Lys Phe 
    50                  55                  60                  


Lys Gly Lys Ala Thr Leu Thr Ala Asp Lys Ser Ser Ser Thr Ala Tyr 
65                  70                  75                  80  


Met Glu Leu Arg Ser Leu Thr Ser Glu Asp Ser Ala Val Tyr Tyr Cys 
                85                  90                  95      


Thr Arg Phe Tyr Ser Tyr Thr Tyr Trp Gly Gln Gly Thr Leu Val Thr 
            100                 105                 110         


Val Ser Ala Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly 
        115                 120                 125             


Gly Ser Asp Val Val Met Thr Gln Thr Pro Leu Ser Leu Pro Val Ser 
    130                 135                 140                 


Leu Gly Asp Gln Ala Ser Ile Ser Cys Arg Ser Ser Gln Ser Leu Val 
145                 150                 155                 160 


His Ser Asn Gly Asn Thr Tyr Leu His Trp Tyr Leu Gln Lys Pro Gly 
                165                 170                 175     


Gln Ser Pro Lys Leu Leu Ile Tyr Lys Val Ser Asn Arg Phe Ser Gly 
            180                 185                 190         


Val Pro Asp Arg Phe Ser Gly Ser Gly Ser Gly Thr Asp Phe Thr Leu 
        195                 200                 205             


Lys Ile Ser Arg Val Glu Ala Glu Asp Leu Gly Val Tyr Phe Cys Ser 
    210                 215                 220                 


Gln Asn Thr His Val Pro Pro Thr Phe Gly Ser Gly Thr Lys Leu Glu 
225                 230                 235                 240 


Ile Lys 
        


<210>  10
<211>  24
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  CD28 transmembrane domain

<400>  10

Phe Trp Val Leu Val Val Val Gly Gly Val Leu Ala Cys Tyr Ser Leu 
1               5                   10                  15      


Leu Val Thr Val Ala Phe Ile Ile 
            20                  


<210>  11
<211>  28
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  CD8alpha transmembrane domain

<400>  11

Ile Tyr Ile Trp Ala Pro Leu Ala Gly Thr Cys Gly Val Leu Leu Leu 
1               5                   10                  15      


Ser Leu Val Ile Thr Leu Tyr Cys Asn His Arg Asn 
            20                  25              


<210>  12
<211>  4
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ITAM motif


<220>
<221>  misc_feature
<222>  (2)..(3)
<223>  Xaa can be any naturally occurring amino acid

<220>
<221>  MISC_FEATURE
<222>  (4)..(4)
<223>  Xaa = Leu or Ile

<400>  12

Tyr Xaa Xaa Xaa 
1               


<210>  13
<211>  11
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ITAM motif


<220>
<221>  misc_feature
<222>  (2)..(3)
<223>  Xaa can be any naturally occurring amino acid

<220>
<221>  MISC_FEATURE
<222>  (4)..(4)
<223>  Xaa = Leu or Ile

<220>
<221>  misc_feature
<222>  (5)..(7)
<223>  Xaa can be any naturally occurring amino acid

<220>
<221>  misc_feature
<222>  (9)..(10)
<223>  Xaa can be any naturally occurring amino acid

<220>
<221>  MISC_FEATURE
<222>  (11)..(11)
<223>  Xaa = Leu or Ile

<400>  13

Tyr Xaa Xaa Xaa Xaa Xaa Xaa Tyr Xaa Xaa Xaa 
1               5                   10      


<210>  14
<211>  112
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  CD3-zeta intracellular domain

<400>  14

Arg Val Lys Phe Ser Arg Ser Ala Asp Ala Pro Ala Tyr Gln Gln Gly 
1               5                   10                  15      


Gln Asn Gln Leu Tyr Asn Glu Leu Asn Leu Gly Arg Arg Glu Glu Tyr 
            20                  25                  30          


Asp Val Leu Asp Lys Arg Arg Gly Arg Asp Pro Glu Met Gly Gly Lys 
        35                  40                  45              


Pro Arg Arg Lys Asn Pro Gln Glu Gly Leu Tyr Asn Glu Leu Gln Lys 
    50                  55                  60                  


Asp Lys Met Ala Glu Ala Tyr Ser Glu Ile Gly Met Lys Gly Glu Arg 
65                  70                  75                  80  


Arg Arg Gly Lys Gly His Asp Gly Leu Tyr Gln Gly Leu Ser Thr Ala 
                85                  90                  95      


Thr Lys Asp Thr Tyr Asp Ala Leu His Met Gln Ala Leu Pro Pro Arg 
            100                 105                 110         


<210>  15
<211>  336
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Human CD226; UniProt Q15762 (CD226_HUMAN)

<400>  15

Met Asp Tyr Pro Thr Leu Leu Leu Ala Leu Leu His Val Tyr Arg Ala 
1               5                   10                  15      


Leu Cys Glu Glu Val Leu Trp His Thr Ser Val Pro Phe Ala Glu Asn 
            20                  25                  30          


Met Ser Leu Glu Cys Val Tyr Pro Ser Met Gly Ile Leu Thr Gln Val 
        35                  40                  45              


Glu Trp Phe Lys Ile Gly Thr Gln Gln Asp Ser Ile Ala Ile Phe Ser 
    50                  55                  60                  


Pro Thr His Gly Met Val Ile Arg Lys Pro Tyr Ala Glu Arg Val Tyr 
65                  70                  75                  80  


Phe Leu Asn Ser Thr Met Ala Ser Asn Asn Met Thr Leu Phe Phe Arg 
                85                  90                  95      


Asn Ala Ser Glu Asp Asp Val Gly Tyr Tyr Ser Cys Ser Leu Tyr Thr 
            100                 105                 110         


Tyr Pro Gln Gly Thr Trp Gln Lys Val Ile Gln Val Val Gln Ser Asp 
        115                 120                 125             


Ser Phe Glu Ala Ala Val Pro Ser Asn Ser His Ile Val Ser Glu Pro 
    130                 135                 140                 


Gly Lys Asn Val Thr Leu Thr Cys Gln Pro Gln Met Thr Trp Pro Val 
145                 150                 155                 160 


Gln Ala Val Arg Trp Glu Lys Ile Gln Pro Arg Gln Ile Asp Leu Leu 
                165                 170                 175     


Thr Tyr Cys Asn Leu Val His Gly Arg Asn Phe Thr Ser Lys Phe Pro 
            180                 185                 190         


Arg Gln Ile Val Ser Asn Cys Ser His Gly Arg Trp Ser Val Ile Val 
        195                 200                 205             


Ile Pro Asp Val Thr Val Ser Asp Ser Gly Leu Tyr Arg Cys Tyr Leu 
    210                 215                 220                 


Gln Ala Ser Ala Gly Glu Asn Glu Thr Phe Val Met Arg Leu Thr Val 
225                 230                 235                 240 


Ala Glu Gly Lys Thr Asp Asn Gln Tyr Thr Leu Phe Val Ala Gly Gly 
                245                 250                 255     


Thr Val Leu Leu Leu Leu Phe Val Ile Ser Ile Thr Thr Ile Ile Val 
            260                 265                 270         


Ile Phe Leu Asn Arg Arg Arg Arg Arg Glu Arg Arg Asp Leu Phe Thr 
        275                 280                 285             


Glu Ser Trp Asp Thr Gln Lys Ala Pro Asn Asn Tyr Arg Ser Pro Ile 
    290                 295                 300                 


Ser Thr Ser Gln Pro Thr Asn Gln Ser Met Asp Asp Thr Arg Glu Asp 
305                 310                 315                 320 


Ile Tyr Val Asn Tyr Pro Thr Phe Ser Arg Arg Pro Lys Thr Arg Val 
                325                 330                 335     


<210>  16
<211>  66
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  CD226 intracellular domain (amino acid positions 271 to 336 of 
       UniProt Q15762)

<400>  16

Ile Val Ile Phe Leu Asn Arg Arg Arg Arg Arg Glu Arg Arg Asp Leu 
1               5                   10                  15      


Phe Thr Glu Ser Trp Asp Thr Gln Lys Ala Pro Asn Asn Tyr Arg Ser 
            20                  25                  30          


Pro Ile Ser Thr Ser Gln Pro Thr Asn Gln Ser Met Asp Asp Thr Arg 
        35                  40                  45              


Glu Asp Ile Tyr Val Asn Tyr Pro Thr Phe Ser Arg Arg Pro Lys Thr 
    50                  55                  60                  


Arg Val 
65      


<210>  17
<211>  44
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  CD28 intracellular domain

<400>  17

Phe Trp Val Arg Ser Lys Arg Ser Arg Leu Leu His Ser Asp Tyr Met 
1               5                   10                  15      


Asn Met Thr Pro Arg Arg Pro Gly Pro Thr Arg Lys His Tyr Gln Pro 
            20                  25                  30          


Tyr Ala Pro Pro Arg Asp Phe Ala Ala Tyr Arg Ser 
        35                  40                  


<210>  18
<211>  42
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  4-1BB intracellular domain

<400>  18

Lys Arg Gly Arg Lys Lys Leu Leu Tyr Ile Phe Lys Gln Pro Phe Met 
1               5                   10                  15      


Arg Pro Val Gln Thr Thr Gln Glu Glu Asp Gly Cys Ser Cys Arg Phe 
            20                  25                  30          


Pro Glu Glu Glu Glu Gly Gly Cys Glu Leu 
        35                  40          


<210>  19
<211>  15
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Human IgG1 hinge region

<400>  19

Glu Pro Lys Ser Cys Asp Lys Thr His Thr Cys Pro Pro Cys Pro 
1               5                   10                  15  


<210>  20
<211>  107
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  F36V-FKBP dimerization sequence

<400>  20

Gly Val Gln Val Glu Thr Ile Ser Pro Gly Asp Gly Arg Thr Phe Pro 
1               5                   10                  15      


Lys Arg Gly Gln Thr Cys Val Val His Tyr Thr Gly Met Leu Glu Asp 
            20                  25                  30          


Gly Lys Lys Val Asp Ser Ser Arg Asp Arg Asn Lys Pro Phe Lys Phe 
        35                  40                  45              


Met Leu Gly Lys Gln Glu Val Ile Arg Gly Trp Glu Glu Gly Val Ala 
    50                  55                  60                  


Gln Met Ser Val Gly Gln Arg Ala Lys Leu Thr Ile Ser Pro Asp Tyr 
65                  70                  75                  80  


Ala Tyr Gly Ala Thr Gly His Pro Gly Ile Ile Pro Pro His Ala Thr 
                85                  90                  95      


Leu Val Phe Asp Val Glu Leu Leu Lys Leu Glu 
            100                 105         


<210>  21
<211>  19
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Human Ig heavy chain signal sequence

<400>  21

Met Asp Trp Ile Trp Arg Ile Leu Phe Leu Val Gly Ala Ala Thr Gly 
1               5                   10                  15      


Ala His Ser 
            


<210>  22
<211>  466
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  scFV GC33/hIgG1 hinge/CD8alpha TMD/CD226/CD3zeta

<400>  22

Gln Val Gln Leu Gln Gln Ser Gly Ala Glu Leu Val Arg Pro Gly Ala 
1               5                   10                  15      


Ser Val Lys Leu Ser Cys Lys Ala Ser Gly Tyr Thr Phe Thr Asp Tyr 
            20                  25                  30          


Glu Met His Trp Val Lys Gln Thr Pro Val His Gly Leu Lys Trp Ile 
        35                  40                  45              


Gly Ala Leu Asp Pro Lys Thr Gly Asp Thr Ala Tyr Ser Gln Lys Phe 
    50                  55                  60                  


Lys Gly Lys Ala Thr Leu Thr Ala Asp Lys Ser Ser Ser Thr Ala Tyr 
65                  70                  75                  80  


Met Glu Leu Arg Ser Leu Thr Ser Glu Asp Ser Ala Val Tyr Tyr Cys 
                85                  90                  95      


Thr Arg Phe Tyr Ser Tyr Thr Tyr Trp Gly Gln Gly Thr Leu Val Thr 
            100                 105                 110         


Val Ser Ala Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly 
        115                 120                 125             


Gly Ser Asp Val Val Met Thr Gln Thr Pro Leu Ser Leu Pro Val Ser 
    130                 135                 140                 


Leu Gly Asp Gln Ala Ser Ile Ser Cys Arg Ser Ser Gln Ser Leu Val 
145                 150                 155                 160 


His Ser Asn Gly Asn Thr Tyr Leu His Trp Tyr Leu Gln Lys Pro Gly 
                165                 170                 175     


Gln Ser Pro Lys Leu Leu Ile Tyr Lys Val Ser Asn Arg Phe Ser Gly 
            180                 185                 190         


Val Pro Asp Arg Phe Ser Gly Ser Gly Ser Gly Thr Asp Phe Thr Leu 
        195                 200                 205             


Lys Ile Ser Arg Val Glu Ala Glu Asp Leu Gly Val Tyr Phe Cys Ser 
    210                 215                 220                 


Gln Asn Thr His Val Pro Pro Thr Phe Gly Ser Gly Thr Lys Leu Glu 
225                 230                 235                 240 


Ile Lys Glu Pro Lys Ser Cys Asp Lys Thr His Thr Cys Pro Pro Cys 
                245                 250                 255     


Pro Asp Pro Lys Ile Tyr Ile Trp Ala Pro Leu Ala Gly Thr Cys Gly 
            260                 265                 270         


Val Leu Leu Leu Ser Leu Val Ile Thr Leu Tyr Cys Asn His Arg Asn 
        275                 280                 285             


Ile Val Ile Phe Leu Asn Arg Arg Arg Arg Arg Glu Arg Arg Asp Leu 
    290                 295                 300                 


Phe Thr Glu Ser Trp Asp Thr Gln Lys Ala Pro Asn Asn Tyr Arg Ser 
305                 310                 315                 320 


Pro Ile Ser Thr Ser Gln Pro Thr Asn Gln Ser Met Asp Asp Thr Arg 
                325                 330                 335     


Glu Asp Ile Tyr Val Asn Tyr Pro Thr Phe Ser Arg Arg Pro Lys Thr 
            340                 345                 350         


Arg Val Arg Val Lys Phe Ser Arg Ser Ala Asp Ala Pro Ala Tyr Gln 
        355                 360                 365             


Gln Gly Gln Asn Gln Leu Tyr Asn Glu Leu Asn Leu Gly Arg Arg Glu 
    370                 375                 380                 


Glu Tyr Asp Val Leu Asp Lys Arg Arg Gly Arg Asp Pro Glu Met Gly 
385                 390                 395                 400 


Gly Lys Pro Arg Arg Lys Asn Pro Gln Glu Gly Leu Tyr Asn Glu Leu 
                405                 410                 415     


Gln Lys Asp Lys Met Ala Glu Ala Tyr Ser Glu Ile Gly Met Lys Gly 
            420                 425                 430         


Glu Arg Arg Arg Gly Lys Gly His Asp Gly Leu Tyr Gln Gly Leu Ser 
        435                 440                 445             


Thr Ala Thr Lys Asp Thr Tyr Asp Ala Leu His Met Gln Ala Leu Pro 
    450                 455                 460                 


Pro Arg 
465     


<210>  23
<211>  573
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  scFV GC33/hIgG1 hinge/CD8alpha TMD/F36V-FKBP/CD226/CD3zeta

<400>  23

Gln Val Gln Leu Gln Gln Ser Gly Ala Glu Leu Val Arg Pro Gly Ala 
1               5                   10                  15      


Ser Val Lys Leu Ser Cys Lys Ala Ser Gly Tyr Thr Phe Thr Asp Tyr 
            20                  25                  30          


Glu Met His Trp Val Lys Gln Thr Pro Val His Gly Leu Lys Trp Ile 
        35                  40                  45              


Gly Ala Leu Asp Pro Lys Thr Gly Asp Thr Ala Tyr Ser Gln Lys Phe 
    50                  55                  60                  


Lys Gly Lys Ala Thr Leu Thr Ala Asp Lys Ser Ser Ser Thr Ala Tyr 
65                  70                  75                  80  


Met Glu Leu Arg Ser Leu Thr Ser Glu Asp Ser Ala Val Tyr Tyr Cys 
                85                  90                  95      


Thr Arg Phe Tyr Ser Tyr Thr Tyr Trp Gly Gln Gly Thr Leu Val Thr 
            100                 105                 110         


Val Ser Ala Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly 
        115                 120                 125             


Gly Ser Asp Val Val Met Thr Gln Thr Pro Leu Ser Leu Pro Val Ser 
    130                 135                 140                 


Leu Gly Asp Gln Ala Ser Ile Ser Cys Arg Ser Ser Gln Ser Leu Val 
145                 150                 155                 160 


His Ser Asn Gly Asn Thr Tyr Leu His Trp Tyr Leu Gln Lys Pro Gly 
                165                 170                 175     


Gln Ser Pro Lys Leu Leu Ile Tyr Lys Val Ser Asn Arg Phe Ser Gly 
            180                 185                 190         


Val Pro Asp Arg Phe Ser Gly Ser Gly Ser Gly Thr Asp Phe Thr Leu 
        195                 200                 205             


Lys Ile Ser Arg Val Glu Ala Glu Asp Leu Gly Val Tyr Phe Cys Ser 
    210                 215                 220                 


Gln Asn Thr His Val Pro Pro Thr Phe Gly Ser Gly Thr Lys Leu Glu 
225                 230                 235                 240 


Ile Lys Glu Pro Lys Ser Cys Asp Lys Thr His Thr Cys Pro Pro Cys 
                245                 250                 255     


Pro Asp Pro Lys Ile Tyr Ile Trp Ala Pro Leu Ala Gly Thr Cys Gly 
            260                 265                 270         


Val Leu Leu Leu Ser Leu Val Ile Thr Leu Tyr Cys Asn His Arg Asn 
        275                 280                 285             


Gly Val Gln Val Glu Thr Ile Ser Pro Gly Asp Gly Arg Thr Phe Pro 
    290                 295                 300                 


Lys Arg Gly Gln Thr Cys Val Val His Tyr Thr Gly Met Leu Glu Asp 
305                 310                 315                 320 


Gly Lys Lys Val Asp Ser Ser Arg Asp Arg Asn Lys Pro Phe Lys Phe 
                325                 330                 335     


Met Leu Gly Lys Gln Glu Val Ile Arg Gly Trp Glu Glu Gly Val Ala 
            340                 345                 350         


Gln Met Ser Val Gly Gln Arg Ala Lys Leu Thr Ile Ser Pro Asp Tyr 
        355                 360                 365             


Ala Tyr Gly Ala Thr Gly His Pro Gly Ile Ile Pro Pro His Ala Thr 
    370                 375                 380                 


Leu Val Phe Asp Val Glu Leu Leu Lys Leu Glu Ile Val Ile Phe Leu 
385                 390                 395                 400 


Asn Arg Arg Arg Arg Arg Glu Arg Arg Asp Leu Phe Thr Glu Ser Trp 
                405                 410                 415     


Asp Thr Gln Lys Ala Pro Asn Asn Tyr Arg Ser Pro Ile Ser Thr Ser 
            420                 425                 430         


Gln Pro Thr Asn Gln Ser Met Asp Asp Thr Arg Glu Asp Ile Tyr Val 
        435                 440                 445             


Asn Tyr Pro Thr Phe Ser Arg Arg Pro Lys Thr Arg Val Arg Val Lys 
    450                 455                 460                 


Phe Ser Arg Ser Ala Asp Ala Pro Ala Tyr Gln Gln Gly Gln Asn Gln 
465                 470                 475                 480 


Leu Tyr Asn Glu Leu Asn Leu Gly Arg Arg Glu Glu Tyr Asp Val Leu 
                485                 490                 495     


Asp Lys Arg Arg Gly Arg Asp Pro Glu Met Gly Gly Lys Pro Arg Arg 
            500                 505                 510         


Lys Asn Pro Gln Glu Gly Leu Tyr Asn Glu Leu Gln Lys Asp Lys Met 
        515                 520                 525             


Ala Glu Ala Tyr Ser Glu Ile Gly Met Lys Gly Glu Arg Arg Arg Gly 
    530                 535                 540                 


Lys Gly His Asp Gly Leu Tyr Gln Gly Leu Ser Thr Ala Thr Lys Asp 
545                 550                 555                 560 


Thr Tyr Asp Ala Leu His Met Gln Ala Leu Pro Pro Arg 
                565                 570             


<210>  24
<211>  510
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  scFV GC33/hIgG1 hinge/CD8alpha TMD/CD226/CD28/CD3zeta

<400>  24

Gln Val Gln Leu Gln Gln Ser Gly Ala Glu Leu Val Arg Pro Gly Ala 
1               5                   10                  15      


Ser Val Lys Leu Ser Cys Lys Ala Ser Gly Tyr Thr Phe Thr Asp Tyr 
            20                  25                  30          


Glu Met His Trp Val Lys Gln Thr Pro Val His Gly Leu Lys Trp Ile 
        35                  40                  45              


Gly Ala Leu Asp Pro Lys Thr Gly Asp Thr Ala Tyr Ser Gln Lys Phe 
    50                  55                  60                  


Lys Gly Lys Ala Thr Leu Thr Ala Asp Lys Ser Ser Ser Thr Ala Tyr 
65                  70                  75                  80  


Met Glu Leu Arg Ser Leu Thr Ser Glu Asp Ser Ala Val Tyr Tyr Cys 
                85                  90                  95      


Thr Arg Phe Tyr Ser Tyr Thr Tyr Trp Gly Gln Gly Thr Leu Val Thr 
            100                 105                 110         


Val Ser Ala Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly 
        115                 120                 125             


Gly Ser Asp Val Val Met Thr Gln Thr Pro Leu Ser Leu Pro Val Ser 
    130                 135                 140                 


Leu Gly Asp Gln Ala Ser Ile Ser Cys Arg Ser Ser Gln Ser Leu Val 
145                 150                 155                 160 


His Ser Asn Gly Asn Thr Tyr Leu His Trp Tyr Leu Gln Lys Pro Gly 
                165                 170                 175     


Gln Ser Pro Lys Leu Leu Ile Tyr Lys Val Ser Asn Arg Phe Ser Gly 
            180                 185                 190         


Val Pro Asp Arg Phe Ser Gly Ser Gly Ser Gly Thr Asp Phe Thr Leu 
        195                 200                 205             


Lys Ile Ser Arg Val Glu Ala Glu Asp Leu Gly Val Tyr Phe Cys Ser 
    210                 215                 220                 


Gln Asn Thr His Val Pro Pro Thr Phe Gly Ser Gly Thr Lys Leu Glu 
225                 230                 235                 240 


Ile Lys Glu Pro Lys Ser Cys Asp Lys Thr His Thr Cys Pro Pro Cys 
                245                 250                 255     


Pro Asp Pro Lys Ile Tyr Ile Trp Ala Pro Leu Ala Gly Thr Cys Gly 
            260                 265                 270         


Val Leu Leu Leu Ser Leu Val Ile Thr Leu Tyr Cys Asn His Arg Asn 
        275                 280                 285             


Ile Val Ile Phe Leu Asn Arg Arg Arg Arg Arg Glu Arg Arg Asp Leu 
    290                 295                 300                 


Phe Thr Glu Ser Trp Asp Thr Gln Lys Ala Pro Asn Asn Tyr Arg Ser 
305                 310                 315                 320 


Pro Ile Ser Thr Ser Gln Pro Thr Asn Gln Ser Met Asp Asp Thr Arg 
                325                 330                 335     


Glu Asp Ile Tyr Val Asn Tyr Pro Thr Phe Ser Arg Arg Pro Lys Thr 
            340                 345                 350         


Arg Val Phe Trp Val Arg Ser Lys Arg Ser Arg Leu Leu His Ser Asp 
        355                 360                 365             


Tyr Met Asn Met Thr Pro Arg Arg Pro Gly Pro Thr Arg Lys His Tyr 
    370                 375                 380                 


Gln Pro Tyr Ala Pro Pro Arg Asp Phe Ala Ala Tyr Arg Ser Arg Val 
385                 390                 395                 400 


Lys Phe Ser Arg Ser Ala Asp Ala Pro Ala Tyr Gln Gln Gly Gln Asn 
                405                 410                 415     


Gln Leu Tyr Asn Glu Leu Asn Leu Gly Arg Arg Glu Glu Tyr Asp Val 
            420                 425                 430         


Leu Asp Lys Arg Arg Gly Arg Asp Pro Glu Met Gly Gly Lys Pro Arg 
        435                 440                 445             


Arg Lys Asn Pro Gln Glu Gly Leu Tyr Asn Glu Leu Gln Lys Asp Lys 
    450                 455                 460                 


Met Ala Glu Ala Tyr Ser Glu Ile Gly Met Lys Gly Glu Arg Arg Arg 
465                 470                 475                 480 


Gly Lys Gly His Asp Gly Leu Tyr Gln Gly Leu Ser Thr Ala Thr Lys 
                485                 490                 495     


Asp Thr Tyr Asp Ala Leu His Met Gln Ala Leu Pro Pro Arg 
            500                 505                 510 


<210>  25
<211>  617
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  scFV GC33/hIgG1 hinge/CD8alpha TMD/F36V-FKBP/CD226/CD28/CD3zeta

<400>  25

Gln Val Gln Leu Gln Gln Ser Gly Ala Glu Leu Val Arg Pro Gly Ala 
1               5                   10                  15      


Ser Val Lys Leu Ser Cys Lys Ala Ser Gly Tyr Thr Phe Thr Asp Tyr 
            20                  25                  30          


Glu Met His Trp Val Lys Gln Thr Pro Val His Gly Leu Lys Trp Ile 
        35                  40                  45              


Gly Ala Leu Asp Pro Lys Thr Gly Asp Thr Ala Tyr Ser Gln Lys Phe 
    50                  55                  60                  


Lys Gly Lys Ala Thr Leu Thr Ala Asp Lys Ser Ser Ser Thr Ala Tyr 
65                  70                  75                  80  


Met Glu Leu Arg Ser Leu Thr Ser Glu Asp Ser Ala Val Tyr Tyr Cys 
                85                  90                  95      


Thr Arg Phe Tyr Ser Tyr Thr Tyr Trp Gly Gln Gly Thr Leu Val Thr 
            100                 105                 110         


Val Ser Ala Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly 
        115                 120                 125             


Gly Ser Asp Val Val Met Thr Gln Thr Pro Leu Ser Leu Pro Val Ser 
    130                 135                 140                 


Leu Gly Asp Gln Ala Ser Ile Ser Cys Arg Ser Ser Gln Ser Leu Val 
145                 150                 155                 160 


His Ser Asn Gly Asn Thr Tyr Leu His Trp Tyr Leu Gln Lys Pro Gly 
                165                 170                 175     


Gln Ser Pro Lys Leu Leu Ile Tyr Lys Val Ser Asn Arg Phe Ser Gly 
            180                 185                 190         


Val Pro Asp Arg Phe Ser Gly Ser Gly Ser Gly Thr Asp Phe Thr Leu 
        195                 200                 205             


Lys Ile Ser Arg Val Glu Ala Glu Asp Leu Gly Val Tyr Phe Cys Ser 
    210                 215                 220                 


Gln Asn Thr His Val Pro Pro Thr Phe Gly Ser Gly Thr Lys Leu Glu 
225                 230                 235                 240 


Ile Lys Glu Pro Lys Ser Cys Asp Lys Thr His Thr Cys Pro Pro Cys 
                245                 250                 255     


Pro Asp Pro Lys Ile Tyr Ile Trp Ala Pro Leu Ala Gly Thr Cys Gly 
            260                 265                 270         


Val Leu Leu Leu Ser Leu Val Ile Thr Leu Tyr Cys Asn His Arg Asn 
        275                 280                 285             


Gly Val Gln Val Glu Thr Ile Ser Pro Gly Asp Gly Arg Thr Phe Pro 
    290                 295                 300                 


Lys Arg Gly Gln Thr Cys Val Val His Tyr Thr Gly Met Leu Glu Asp 
305                 310                 315                 320 


Gly Lys Lys Val Asp Ser Ser Arg Asp Arg Asn Lys Pro Phe Lys Phe 
                325                 330                 335     


Met Leu Gly Lys Gln Glu Val Ile Arg Gly Trp Glu Glu Gly Val Ala 
            340                 345                 350         


Gln Met Ser Val Gly Gln Arg Ala Lys Leu Thr Ile Ser Pro Asp Tyr 
        355                 360                 365             


Ala Tyr Gly Ala Thr Gly His Pro Gly Ile Ile Pro Pro His Ala Thr 
    370                 375                 380                 


Leu Val Phe Asp Val Glu Leu Leu Lys Leu Glu Ile Val Ile Phe Leu 
385                 390                 395                 400 


Asn Arg Arg Arg Arg Arg Glu Arg Arg Asp Leu Phe Thr Glu Ser Trp 
                405                 410                 415     


Asp Thr Gln Lys Ala Pro Asn Asn Tyr Arg Ser Pro Ile Ser Thr Ser 
            420                 425                 430         


Gln Pro Thr Asn Gln Ser Met Asp Asp Thr Arg Glu Asp Ile Tyr Val 
        435                 440                 445             


Asn Tyr Pro Thr Phe Ser Arg Arg Pro Lys Thr Arg Val Phe Trp Val 
    450                 455                 460                 


Arg Ser Lys Arg Ser Arg Leu Leu His Ser Asp Tyr Met Asn Met Thr 
465                 470                 475                 480 


Pro Arg Arg Pro Gly Pro Thr Arg Lys His Tyr Gln Pro Tyr Ala Pro 
                485                 490                 495     


Pro Arg Asp Phe Ala Ala Tyr Arg Ser Arg Val Lys Phe Ser Arg Ser 
            500                 505                 510         


Ala Asp Ala Pro Ala Tyr Gln Gln Gly Gln Asn Gln Leu Tyr Asn Glu 
        515                 520                 525             


Leu Asn Leu Gly Arg Arg Glu Glu Tyr Asp Val Leu Asp Lys Arg Arg 
    530                 535                 540                 


Gly Arg Asp Pro Glu Met Gly Gly Lys Pro Arg Arg Lys Asn Pro Gln 
545                 550                 555                 560 


Glu Gly Leu Tyr Asn Glu Leu Gln Lys Asp Lys Met Ala Glu Ala Tyr 
                565                 570                 575     


Ser Glu Ile Gly Met Lys Gly Glu Arg Arg Arg Gly Lys Gly His Asp 
            580                 585                 590         


Gly Leu Tyr Gln Gly Leu Ser Thr Ala Thr Lys Asp Thr Tyr Asp Ala 
        595                 600                 605             


Leu His Met Gln Ala Leu Pro Pro Arg 
    610                 615         


<210>  26
<211>  508
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  scFV GC33/hIgG1 hinge/CD8alpha TMD/CD226/4-1BB/CD3zeta

<400>  26

Gln Val Gln Leu Gln Gln Ser Gly Ala Glu Leu Val Arg Pro Gly Ala 
1               5                   10                  15      


Ser Val Lys Leu Ser Cys Lys Ala Ser Gly Tyr Thr Phe Thr Asp Tyr 
            20                  25                  30          


Glu Met His Trp Val Lys Gln Thr Pro Val His Gly Leu Lys Trp Ile 
        35                  40                  45              


Gly Ala Leu Asp Pro Lys Thr Gly Asp Thr Ala Tyr Ser Gln Lys Phe 
    50                  55                  60                  


Lys Gly Lys Ala Thr Leu Thr Ala Asp Lys Ser Ser Ser Thr Ala Tyr 
65                  70                  75                  80  


Met Glu Leu Arg Ser Leu Thr Ser Glu Asp Ser Ala Val Tyr Tyr Cys 
                85                  90                  95      


Thr Arg Phe Tyr Ser Tyr Thr Tyr Trp Gly Gln Gly Thr Leu Val Thr 
            100                 105                 110         


Val Ser Ala Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly 
        115                 120                 125             


Gly Ser Asp Val Val Met Thr Gln Thr Pro Leu Ser Leu Pro Val Ser 
    130                 135                 140                 


Leu Gly Asp Gln Ala Ser Ile Ser Cys Arg Ser Ser Gln Ser Leu Val 
145                 150                 155                 160 


His Ser Asn Gly Asn Thr Tyr Leu His Trp Tyr Leu Gln Lys Pro Gly 
                165                 170                 175     


Gln Ser Pro Lys Leu Leu Ile Tyr Lys Val Ser Asn Arg Phe Ser Gly 
            180                 185                 190         


Val Pro Asp Arg Phe Ser Gly Ser Gly Ser Gly Thr Asp Phe Thr Leu 
        195                 200                 205             


Lys Ile Ser Arg Val Glu Ala Glu Asp Leu Gly Val Tyr Phe Cys Ser 
    210                 215                 220                 


Gln Asn Thr His Val Pro Pro Thr Phe Gly Ser Gly Thr Lys Leu Glu 
225                 230                 235                 240 


Ile Lys Glu Pro Lys Ser Cys Asp Lys Thr His Thr Cys Pro Pro Cys 
                245                 250                 255     


Pro Asp Pro Lys Ile Tyr Ile Trp Ala Pro Leu Ala Gly Thr Cys Gly 
            260                 265                 270         


Val Leu Leu Leu Ser Leu Val Ile Thr Leu Tyr Cys Asn His Arg Asn 
        275                 280                 285             


Ile Val Ile Phe Leu Asn Arg Arg Arg Arg Arg Glu Arg Arg Asp Leu 
    290                 295                 300                 


Phe Thr Glu Ser Trp Asp Thr Gln Lys Ala Pro Asn Asn Tyr Arg Ser 
305                 310                 315                 320 


Pro Ile Ser Thr Ser Gln Pro Thr Asn Gln Ser Met Asp Asp Thr Arg 
                325                 330                 335     


Glu Asp Ile Tyr Val Asn Tyr Pro Thr Phe Ser Arg Arg Pro Lys Thr 
            340                 345                 350         


Arg Val Lys Arg Gly Arg Lys Lys Leu Leu Tyr Ile Phe Lys Gln Pro 
        355                 360                 365             


Phe Met Arg Pro Val Gln Thr Thr Gln Glu Glu Asp Gly Cys Ser Cys 
    370                 375                 380                 


Arg Phe Pro Glu Glu Glu Glu Gly Gly Cys Glu Leu Arg Val Lys Phe 
385                 390                 395                 400 


Ser Arg Ser Ala Asp Ala Pro Ala Tyr Gln Gln Gly Gln Asn Gln Leu 
                405                 410                 415     


Tyr Asn Glu Leu Asn Leu Gly Arg Arg Glu Glu Tyr Asp Val Leu Asp 
            420                 425                 430         


Lys Arg Arg Gly Arg Asp Pro Glu Met Gly Gly Lys Pro Arg Arg Lys 
        435                 440                 445             


Asn Pro Gln Glu Gly Leu Tyr Asn Glu Leu Gln Lys Asp Lys Met Ala 
    450                 455                 460                 


Glu Ala Tyr Ser Glu Ile Gly Met Lys Gly Glu Arg Arg Arg Gly Lys 
465                 470                 475                 480 


Gly His Asp Gly Leu Tyr Gln Gly Leu Ser Thr Ala Thr Lys Asp Thr 
                485                 490                 495     


Tyr Asp Ala Leu His Met Gln Ala Leu Pro Pro Arg 
            500                 505             


<210>  27
<211>  615
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  scFV GC33/hIgG1 hinge/CD8alpha TMD/F36V-FKBP/CD226/41BB/CD3zeta

<400>  27

Gln Val Gln Leu Gln Gln Ser Gly Ala Glu Leu Val Arg Pro Gly Ala 
1               5                   10                  15      


Ser Val Lys Leu Ser Cys Lys Ala Ser Gly Tyr Thr Phe Thr Asp Tyr 
            20                  25                  30          


Glu Met His Trp Val Lys Gln Thr Pro Val His Gly Leu Lys Trp Ile 
        35                  40                  45              


Gly Ala Leu Asp Pro Lys Thr Gly Asp Thr Ala Tyr Ser Gln Lys Phe 
    50                  55                  60                  


Lys Gly Lys Ala Thr Leu Thr Ala Asp Lys Ser Ser Ser Thr Ala Tyr 
65                  70                  75                  80  


Met Glu Leu Arg Ser Leu Thr Ser Glu Asp Ser Ala Val Tyr Tyr Cys 
                85                  90                  95      


Thr Arg Phe Tyr Ser Tyr Thr Tyr Trp Gly Gln Gly Thr Leu Val Thr 
            100                 105                 110         


Val Ser Ala Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly 
        115                 120                 125             


Gly Ser Asp Val Val Met Thr Gln Thr Pro Leu Ser Leu Pro Val Ser 
    130                 135                 140                 


Leu Gly Asp Gln Ala Ser Ile Ser Cys Arg Ser Ser Gln Ser Leu Val 
145                 150                 155                 160 


His Ser Asn Gly Asn Thr Tyr Leu His Trp Tyr Leu Gln Lys Pro Gly 
                165                 170                 175     


Gln Ser Pro Lys Leu Leu Ile Tyr Lys Val Ser Asn Arg Phe Ser Gly 
            180                 185                 190         


Val Pro Asp Arg Phe Ser Gly Ser Gly Ser Gly Thr Asp Phe Thr Leu 
        195                 200                 205             


Lys Ile Ser Arg Val Glu Ala Glu Asp Leu Gly Val Tyr Phe Cys Ser 
    210                 215                 220                 


Gln Asn Thr His Val Pro Pro Thr Phe Gly Ser Gly Thr Lys Leu Glu 
225                 230                 235                 240 


Ile Lys Glu Pro Lys Ser Cys Asp Lys Thr His Thr Cys Pro Pro Cys 
                245                 250                 255     


Pro Asp Pro Lys Ile Tyr Ile Trp Ala Pro Leu Ala Gly Thr Cys Gly 
            260                 265                 270         


Val Leu Leu Leu Ser Leu Val Ile Thr Leu Tyr Cys Asn His Arg Asn 
        275                 280                 285             


Gly Val Gln Val Glu Thr Ile Ser Pro Gly Asp Gly Arg Thr Phe Pro 
    290                 295                 300                 


Lys Arg Gly Gln Thr Cys Val Val His Tyr Thr Gly Met Leu Glu Asp 
305                 310                 315                 320 


Gly Lys Lys Val Asp Ser Ser Arg Asp Arg Asn Lys Pro Phe Lys Phe 
                325                 330                 335     


Met Leu Gly Lys Gln Glu Val Ile Arg Gly Trp Glu Glu Gly Val Ala 
            340                 345                 350         


Gln Met Ser Val Gly Gln Arg Ala Lys Leu Thr Ile Ser Pro Asp Tyr 
        355                 360                 365             


Ala Tyr Gly Ala Thr Gly His Pro Gly Ile Ile Pro Pro His Ala Thr 
    370                 375                 380                 


Leu Val Phe Asp Val Glu Leu Leu Lys Leu Glu Ile Val Ile Phe Leu 
385                 390                 395                 400 


Asn Arg Arg Arg Arg Arg Glu Arg Arg Asp Leu Phe Thr Glu Ser Trp 
                405                 410                 415     


Asp Thr Gln Lys Ala Pro Asn Asn Tyr Arg Ser Pro Ile Ser Thr Ser 
            420                 425                 430         


Gln Pro Thr Asn Gln Ser Met Asp Asp Thr Arg Glu Asp Ile Tyr Val 
        435                 440                 445             


Asn Tyr Pro Thr Phe Ser Arg Arg Pro Lys Thr Arg Val Lys Arg Gly 
    450                 455                 460                 


Arg Lys Lys Leu Leu Tyr Ile Phe Lys Gln Pro Phe Met Arg Pro Val 
465                 470                 475                 480 


Gln Thr Thr Gln Glu Glu Asp Gly Cys Ser Cys Arg Phe Pro Glu Glu 
                485                 490                 495     


Glu Glu Gly Gly Cys Glu Leu Arg Val Lys Phe Ser Arg Ser Ala Asp 
            500                 505                 510         


Ala Pro Ala Tyr Gln Gln Gly Gln Asn Gln Leu Tyr Asn Glu Leu Asn 
        515                 520                 525             


Leu Gly Arg Arg Glu Glu Tyr Asp Val Leu Asp Lys Arg Arg Gly Arg 
    530                 535                 540                 


Asp Pro Glu Met Gly Gly Lys Pro Arg Arg Lys Asn Pro Gln Glu Gly 
545                 550                 555                 560 


Leu Tyr Asn Glu Leu Gln Lys Asp Lys Met Ala Glu Ala Tyr Ser Glu 
                565                 570                 575     


Ile Gly Met Lys Gly Glu Arg Arg Arg Gly Lys Gly His Asp Gly Leu 
            580                 585                 590         


Tyr Gln Gly Leu Ser Thr Ala Thr Lys Asp Thr Tyr Asp Ala Leu His 
        595                 600                 605             


Met Gln Ala Leu Pro Pro Arg 
    610                 615 


<210>  28
<211>  552
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  scFV GC33/hIgG1 hinge/CD8alpha TMD/CD226/CD28/4-1BB/CD3zeta

<400>  28

Gln Val Gln Leu Gln Gln Ser Gly Ala Glu Leu Val Arg Pro Gly Ala 
1               5                   10                  15      


Ser Val Lys Leu Ser Cys Lys Ala Ser Gly Tyr Thr Phe Thr Asp Tyr 
            20                  25                  30          


Glu Met His Trp Val Lys Gln Thr Pro Val His Gly Leu Lys Trp Ile 
        35                  40                  45              


Gly Ala Leu Asp Pro Lys Thr Gly Asp Thr Ala Tyr Ser Gln Lys Phe 
    50                  55                  60                  


Lys Gly Lys Ala Thr Leu Thr Ala Asp Lys Ser Ser Ser Thr Ala Tyr 
65                  70                  75                  80  


Met Glu Leu Arg Ser Leu Thr Ser Glu Asp Ser Ala Val Tyr Tyr Cys 
                85                  90                  95      


Thr Arg Phe Tyr Ser Tyr Thr Tyr Trp Gly Gln Gly Thr Leu Val Thr 
            100                 105                 110         


Val Ser Ala Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly 
        115                 120                 125             


Gly Ser Asp Val Val Met Thr Gln Thr Pro Leu Ser Leu Pro Val Ser 
    130                 135                 140                 


Leu Gly Asp Gln Ala Ser Ile Ser Cys Arg Ser Ser Gln Ser Leu Val 
145                 150                 155                 160 


His Ser Asn Gly Asn Thr Tyr Leu His Trp Tyr Leu Gln Lys Pro Gly 
                165                 170                 175     


Gln Ser Pro Lys Leu Leu Ile Tyr Lys Val Ser Asn Arg Phe Ser Gly 
            180                 185                 190         


Val Pro Asp Arg Phe Ser Gly Ser Gly Ser Gly Thr Asp Phe Thr Leu 
        195                 200                 205             


Lys Ile Ser Arg Val Glu Ala Glu Asp Leu Gly Val Tyr Phe Cys Ser 
    210                 215                 220                 


Gln Asn Thr His Val Pro Pro Thr Phe Gly Ser Gly Thr Lys Leu Glu 
225                 230                 235                 240 


Ile Lys Glu Pro Lys Ser Cys Asp Lys Thr His Thr Cys Pro Pro Cys 
                245                 250                 255     


Pro Asp Pro Lys Ile Tyr Ile Trp Ala Pro Leu Ala Gly Thr Cys Gly 
            260                 265                 270         


Val Leu Leu Leu Ser Leu Val Ile Thr Leu Tyr Cys Asn His Arg Asn 
        275                 280                 285             


Ile Val Ile Phe Leu Asn Arg Arg Arg Arg Arg Glu Arg Arg Asp Leu 
    290                 295                 300                 


Phe Thr Glu Ser Trp Asp Thr Gln Lys Ala Pro Asn Asn Tyr Arg Ser 
305                 310                 315                 320 


Pro Ile Ser Thr Ser Gln Pro Thr Asn Gln Ser Met Asp Asp Thr Arg 
                325                 330                 335     


Glu Asp Ile Tyr Val Asn Tyr Pro Thr Phe Ser Arg Arg Pro Lys Thr 
            340                 345                 350         


Arg Val Phe Trp Val Arg Ser Lys Arg Ser Arg Leu Leu His Ser Asp 
        355                 360                 365             


Tyr Met Asn Met Thr Pro Arg Arg Pro Gly Pro Thr Arg Lys His Tyr 
    370                 375                 380                 


Gln Pro Tyr Ala Pro Pro Arg Asp Phe Ala Ala Tyr Arg Ser Lys Arg 
385                 390                 395                 400 


Gly Arg Lys Lys Leu Leu Tyr Ile Phe Lys Gln Pro Phe Met Arg Pro 
                405                 410                 415     


Val Gln Thr Thr Gln Glu Glu Asp Gly Cys Ser Cys Arg Phe Pro Glu 
            420                 425                 430         


Glu Glu Glu Gly Gly Cys Glu Leu Arg Val Lys Phe Ser Arg Ser Ala 
        435                 440                 445             


Asp Ala Pro Ala Tyr Gln Gln Gly Gln Asn Gln Leu Tyr Asn Glu Leu 
    450                 455                 460                 


Asn Leu Gly Arg Arg Glu Glu Tyr Asp Val Leu Asp Lys Arg Arg Gly 
465                 470                 475                 480 


Arg Asp Pro Glu Met Gly Gly Lys Pro Arg Arg Lys Asn Pro Gln Glu 
                485                 490                 495     


Gly Leu Tyr Asn Glu Leu Gln Lys Asp Lys Met Ala Glu Ala Tyr Ser 
            500                 505                 510         


Glu Ile Gly Met Lys Gly Glu Arg Arg Arg Gly Lys Gly His Asp Gly 
        515                 520                 525             


Leu Tyr Gln Gly Leu Ser Thr Ala Thr Lys Asp Thr Tyr Asp Ala Leu 
    530                 535                 540                 


His Met Gln Ala Leu Pro Pro Arg 
545                 550         


<210>  29
<211>  659
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  scFV GC33/hIgG1 hinge/CD8alpha 
       TMD/F36V-FKBP/CD226/CD28/41BB/CD3zeta

<400>  29

Gln Val Gln Leu Gln Gln Ser Gly Ala Glu Leu Val Arg Pro Gly Ala 
1               5                   10                  15      


Ser Val Lys Leu Ser Cys Lys Ala Ser Gly Tyr Thr Phe Thr Asp Tyr 
            20                  25                  30          


Glu Met His Trp Val Lys Gln Thr Pro Val His Gly Leu Lys Trp Ile 
        35                  40                  45              


Gly Ala Leu Asp Pro Lys Thr Gly Asp Thr Ala Tyr Ser Gln Lys Phe 
    50                  55                  60                  


Lys Gly Lys Ala Thr Leu Thr Ala Asp Lys Ser Ser Ser Thr Ala Tyr 
65                  70                  75                  80  


Met Glu Leu Arg Ser Leu Thr Ser Glu Asp Ser Ala Val Tyr Tyr Cys 
                85                  90                  95      


Thr Arg Phe Tyr Ser Tyr Thr Tyr Trp Gly Gln Gly Thr Leu Val Thr 
            100                 105                 110         


Val Ser Ala Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly 
        115                 120                 125             


Gly Ser Asp Val Val Met Thr Gln Thr Pro Leu Ser Leu Pro Val Ser 
    130                 135                 140                 


Leu Gly Asp Gln Ala Ser Ile Ser Cys Arg Ser Ser Gln Ser Leu Val 
145                 150                 155                 160 


His Ser Asn Gly Asn Thr Tyr Leu His Trp Tyr Leu Gln Lys Pro Gly 
                165                 170                 175     


Gln Ser Pro Lys Leu Leu Ile Tyr Lys Val Ser Asn Arg Phe Ser Gly 
            180                 185                 190         


Val Pro Asp Arg Phe Ser Gly Ser Gly Ser Gly Thr Asp Phe Thr Leu 
        195                 200                 205             


Lys Ile Ser Arg Val Glu Ala Glu Asp Leu Gly Val Tyr Phe Cys Ser 
    210                 215                 220                 


Gln Asn Thr His Val Pro Pro Thr Phe Gly Ser Gly Thr Lys Leu Glu 
225                 230                 235                 240 


Ile Lys Glu Pro Lys Ser Cys Asp Lys Thr His Thr Cys Pro Pro Cys 
                245                 250                 255     


Pro Asp Pro Lys Ile Tyr Ile Trp Ala Pro Leu Ala Gly Thr Cys Gly 
            260                 265                 270         


Val Leu Leu Leu Ser Leu Val Ile Thr Leu Tyr Cys Asn His Arg Asn 
        275                 280                 285             


Gly Val Gln Val Glu Thr Ile Ser Pro Gly Asp Gly Arg Thr Phe Pro 
    290                 295                 300                 


Lys Arg Gly Gln Thr Cys Val Val His Tyr Thr Gly Met Leu Glu Asp 
305                 310                 315                 320 


Gly Lys Lys Val Asp Ser Ser Arg Asp Arg Asn Lys Pro Phe Lys Phe 
                325                 330                 335     


Met Leu Gly Lys Gln Glu Val Ile Arg Gly Trp Glu Glu Gly Val Ala 
            340                 345                 350         


Gln Met Ser Val Gly Gln Arg Ala Lys Leu Thr Ile Ser Pro Asp Tyr 
        355                 360                 365             


Ala Tyr Gly Ala Thr Gly His Pro Gly Ile Ile Pro Pro His Ala Thr 
    370                 375                 380                 


Leu Val Phe Asp Val Glu Leu Leu Lys Leu Glu Ile Val Ile Phe Leu 
385                 390                 395                 400 


Asn Arg Arg Arg Arg Arg Glu Arg Arg Asp Leu Phe Thr Glu Ser Trp 
                405                 410                 415     


Asp Thr Gln Lys Ala Pro Asn Asn Tyr Arg Ser Pro Ile Ser Thr Ser 
            420                 425                 430         


Gln Pro Thr Asn Gln Ser Met Asp Asp Thr Arg Glu Asp Ile Tyr Val 
        435                 440                 445             


Asn Tyr Pro Thr Phe Ser Arg Arg Pro Lys Thr Arg Val Phe Trp Val 
    450                 455                 460                 


Arg Ser Lys Arg Ser Arg Leu Leu His Ser Asp Tyr Met Asn Met Thr 
465                 470                 475                 480 


Pro Arg Arg Pro Gly Pro Thr Arg Lys His Tyr Gln Pro Tyr Ala Pro 
                485                 490                 495     


Pro Arg Asp Phe Ala Ala Tyr Arg Ser Lys Arg Gly Arg Lys Lys Leu 
            500                 505                 510         


Leu Tyr Ile Phe Lys Gln Pro Phe Met Arg Pro Val Gln Thr Thr Gln 
        515                 520                 525             


Glu Glu Asp Gly Cys Ser Cys Arg Phe Pro Glu Glu Glu Glu Gly Gly 
    530                 535                 540                 


Cys Glu Leu Arg Val Lys Phe Ser Arg Ser Ala Asp Ala Pro Ala Tyr 
545                 550                 555                 560 


Gln Gln Gly Gln Asn Gln Leu Tyr Asn Glu Leu Asn Leu Gly Arg Arg 
                565                 570                 575     


Glu Glu Tyr Asp Val Leu Asp Lys Arg Arg Gly Arg Asp Pro Glu Met 
            580                 585                 590         


Gly Gly Lys Pro Arg Arg Lys Asn Pro Gln Glu Gly Leu Tyr Asn Glu 
        595                 600                 605             


Leu Gln Lys Asp Lys Met Ala Glu Ala Tyr Ser Glu Ile Gly Met Lys 
    610                 615                 620                 


Gly Glu Arg Arg Arg Gly Lys Gly His Asp Gly Leu Tyr Gln Gly Leu 
625                 630                 635                 640 


Ser Thr Ala Thr Lys Asp Thr Tyr Asp Ala Leu His Met Gln Ala Leu 
                645                 650                 655     


Pro Pro Arg 
            


<210>  30
<211>  485
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  hlgG heavy chain signal sequence/scFV GC33/hIgG1 hinge/CD8alpha 
       TMD/CD226/CD3zeta

<400>  30

Met Asp Trp Ile Trp Arg Ile Leu Phe Leu Val Gly Ala Ala Thr Gly 
1               5                   10                  15      


Ala His Ser Gln Val Gln Leu Gln Gln Ser Gly Ala Glu Leu Val Arg 
            20                  25                  30          


Pro Gly Ala Ser Val Lys Leu Ser Cys Lys Ala Ser Gly Tyr Thr Phe 
        35                  40                  45              


Thr Asp Tyr Glu Met His Trp Val Lys Gln Thr Pro Val His Gly Leu 
    50                  55                  60                  


Lys Trp Ile Gly Ala Leu Asp Pro Lys Thr Gly Asp Thr Ala Tyr Ser 
65                  70                  75                  80  


Gln Lys Phe Lys Gly Lys Ala Thr Leu Thr Ala Asp Lys Ser Ser Ser 
                85                  90                  95      


Thr Ala Tyr Met Glu Leu Arg Ser Leu Thr Ser Glu Asp Ser Ala Val 
            100                 105                 110         


Tyr Tyr Cys Thr Arg Phe Tyr Ser Tyr Thr Tyr Trp Gly Gln Gly Thr 
        115                 120                 125             


Leu Val Thr Val Ser Ala Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser 
    130                 135                 140                 


Gly Gly Gly Gly Ser Asp Val Val Met Thr Gln Thr Pro Leu Ser Leu 
145                 150                 155                 160 


Pro Val Ser Leu Gly Asp Gln Ala Ser Ile Ser Cys Arg Ser Ser Gln 
                165                 170                 175     


Ser Leu Val His Ser Asn Gly Asn Thr Tyr Leu His Trp Tyr Leu Gln 
            180                 185                 190         


Lys Pro Gly Gln Ser Pro Lys Leu Leu Ile Tyr Lys Val Ser Asn Arg 
        195                 200                 205             


Phe Ser Gly Val Pro Asp Arg Phe Ser Gly Ser Gly Ser Gly Thr Asp 
    210                 215                 220                 


Phe Thr Leu Lys Ile Ser Arg Val Glu Ala Glu Asp Leu Gly Val Tyr 
225                 230                 235                 240 


Phe Cys Ser Gln Asn Thr His Val Pro Pro Thr Phe Gly Ser Gly Thr 
                245                 250                 255     


Lys Leu Glu Ile Lys Glu Pro Lys Ser Cys Asp Lys Thr His Thr Cys 
            260                 265                 270         


Pro Pro Cys Pro Asp Pro Lys Ile Tyr Ile Trp Ala Pro Leu Ala Gly 
        275                 280                 285             


Thr Cys Gly Val Leu Leu Leu Ser Leu Val Ile Thr Leu Tyr Cys Asn 
    290                 295                 300                 


His Arg Asn Ile Val Ile Phe Leu Asn Arg Arg Arg Arg Arg Glu Arg 
305                 310                 315                 320 


Arg Asp Leu Phe Thr Glu Ser Trp Asp Thr Gln Lys Ala Pro Asn Asn 
                325                 330                 335     


Tyr Arg Ser Pro Ile Ser Thr Ser Gln Pro Thr Asn Gln Ser Met Asp 
            340                 345                 350         


Asp Thr Arg Glu Asp Ile Tyr Val Asn Tyr Pro Thr Phe Ser Arg Arg 
        355                 360                 365             


Pro Lys Thr Arg Val Arg Val Lys Phe Ser Arg Ser Ala Asp Ala Pro 
    370                 375                 380                 


Ala Tyr Gln Gln Gly Gln Asn Gln Leu Tyr Asn Glu Leu Asn Leu Gly 
385                 390                 395                 400 


Arg Arg Glu Glu Tyr Asp Val Leu Asp Lys Arg Arg Gly Arg Asp Pro 
                405                 410                 415     


Glu Met Gly Gly Lys Pro Arg Arg Lys Asn Pro Gln Glu Gly Leu Tyr 
            420                 425                 430         


Asn Glu Leu Gln Lys Asp Lys Met Ala Glu Ala Tyr Ser Glu Ile Gly 
        435                 440                 445             


Met Lys Gly Glu Arg Arg Arg Gly Lys Gly His Asp Gly Leu Tyr Gln 
    450                 455                 460                 


Gly Leu Ser Thr Ala Thr Lys Asp Thr Tyr Asp Ala Leu His Met Gln 
465                 470                 475                 480 


Ala Leu Pro Pro Arg 
                485 


<210>  31
<211>  592
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  hIgG heavy chain signal sequence/scFV GC33/hIgG1 hinge/CD8alpha 
       TMD/F36V-FKBP/CD226/CD3zeta

<400>  31

Met Asp Trp Ile Trp Arg Ile Leu Phe Leu Val Gly Ala Ala Thr Gly 
1               5                   10                  15      


Ala His Ser Gln Val Gln Leu Gln Gln Ser Gly Ala Glu Leu Val Arg 
            20                  25                  30          


Pro Gly Ala Ser Val Lys Leu Ser Cys Lys Ala Ser Gly Tyr Thr Phe 
        35                  40                  45              


Thr Asp Tyr Glu Met His Trp Val Lys Gln Thr Pro Val His Gly Leu 
    50                  55                  60                  


Lys Trp Ile Gly Ala Leu Asp Pro Lys Thr Gly Asp Thr Ala Tyr Ser 
65                  70                  75                  80  


Gln Lys Phe Lys Gly Lys Ala Thr Leu Thr Ala Asp Lys Ser Ser Ser 
                85                  90                  95      


Thr Ala Tyr Met Glu Leu Arg Ser Leu Thr Ser Glu Asp Ser Ala Val 
            100                 105                 110         


Tyr Tyr Cys Thr Arg Phe Tyr Ser Tyr Thr Tyr Trp Gly Gln Gly Thr 
        115                 120                 125             


Leu Val Thr Val Ser Ala Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser 
    130                 135                 140                 


Gly Gly Gly Gly Ser Asp Val Val Met Thr Gln Thr Pro Leu Ser Leu 
145                 150                 155                 160 


Pro Val Ser Leu Gly Asp Gln Ala Ser Ile Ser Cys Arg Ser Ser Gln 
                165                 170                 175     


Ser Leu Val His Ser Asn Gly Asn Thr Tyr Leu His Trp Tyr Leu Gln 
            180                 185                 190         


Lys Pro Gly Gln Ser Pro Lys Leu Leu Ile Tyr Lys Val Ser Asn Arg 
        195                 200                 205             


Phe Ser Gly Val Pro Asp Arg Phe Ser Gly Ser Gly Ser Gly Thr Asp 
    210                 215                 220                 


Phe Thr Leu Lys Ile Ser Arg Val Glu Ala Glu Asp Leu Gly Val Tyr 
225                 230                 235                 240 


Phe Cys Ser Gln Asn Thr His Val Pro Pro Thr Phe Gly Ser Gly Thr 
                245                 250                 255     


Lys Leu Glu Ile Lys Glu Pro Lys Ser Cys Asp Lys Thr His Thr Cys 
            260                 265                 270         


Pro Pro Cys Pro Asp Pro Lys Ile Tyr Ile Trp Ala Pro Leu Ala Gly 
        275                 280                 285             


Thr Cys Gly Val Leu Leu Leu Ser Leu Val Ile Thr Leu Tyr Cys Asn 
    290                 295                 300                 


His Arg Asn Gly Val Gln Val Glu Thr Ile Ser Pro Gly Asp Gly Arg 
305                 310                 315                 320 


Thr Phe Pro Lys Arg Gly Gln Thr Cys Val Val His Tyr Thr Gly Met 
                325                 330                 335     


Leu Glu Asp Gly Lys Lys Val Asp Ser Ser Arg Asp Arg Asn Lys Pro 
            340                 345                 350         


Phe Lys Phe Met Leu Gly Lys Gln Glu Val Ile Arg Gly Trp Glu Glu 
        355                 360                 365             


Gly Val Ala Gln Met Ser Val Gly Gln Arg Ala Lys Leu Thr Ile Ser 
    370                 375                 380                 


Pro Asp Tyr Ala Tyr Gly Ala Thr Gly His Pro Gly Ile Ile Pro Pro 
385                 390                 395                 400 


His Ala Thr Leu Val Phe Asp Val Glu Leu Leu Lys Leu Glu Ile Val 
                405                 410                 415     


Ile Phe Leu Asn Arg Arg Arg Arg Arg Glu Arg Arg Asp Leu Phe Thr 
            420                 425                 430         


Glu Ser Trp Asp Thr Gln Lys Ala Pro Asn Asn Tyr Arg Ser Pro Ile 
        435                 440                 445             


Ser Thr Ser Gln Pro Thr Asn Gln Ser Met Asp Asp Thr Arg Glu Asp 
    450                 455                 460                 


Ile Tyr Val Asn Tyr Pro Thr Phe Ser Arg Arg Pro Lys Thr Arg Val 
465                 470                 475                 480 


Arg Val Lys Phe Ser Arg Ser Ala Asp Ala Pro Ala Tyr Gln Gln Gly 
                485                 490                 495     


Gln Asn Gln Leu Tyr Asn Glu Leu Asn Leu Gly Arg Arg Glu Glu Tyr 
            500                 505                 510         


Asp Val Leu Asp Lys Arg Arg Gly Arg Asp Pro Glu Met Gly Gly Lys 
        515                 520                 525             


Pro Arg Arg Lys Asn Pro Gln Glu Gly Leu Tyr Asn Glu Leu Gln Lys 
    530                 535                 540                 


Asp Lys Met Ala Glu Ala Tyr Ser Glu Ile Gly Met Lys Gly Glu Arg 
545                 550                 555                 560 


Arg Arg Gly Lys Gly His Asp Gly Leu Tyr Gln Gly Leu Ser Thr Ala 
                565                 570                 575     


Thr Lys Asp Thr Tyr Asp Ala Leu His Met Gln Ala Leu Pro Pro Arg 
            580                 585                 590         


<210>  32
<211>  529
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  hIgG heavy chain signal sequence/scFV GC33/hIgG1 hinge/CD8alpha 
       TMD/CD226/CD28/CD3zeta

<400>  32

Met Asp Trp Ile Trp Arg Ile Leu Phe Leu Val Gly Ala Ala Thr Gly 
1               5                   10                  15      


Ala His Ser Gln Val Gln Leu Gln Gln Ser Gly Ala Glu Leu Val Arg 
            20                  25                  30          


Pro Gly Ala Ser Val Lys Leu Ser Cys Lys Ala Ser Gly Tyr Thr Phe 
        35                  40                  45              


Thr Asp Tyr Glu Met His Trp Val Lys Gln Thr Pro Val His Gly Leu 
    50                  55                  60                  


Lys Trp Ile Gly Ala Leu Asp Pro Lys Thr Gly Asp Thr Ala Tyr Ser 
65                  70                  75                  80  


Gln Lys Phe Lys Gly Lys Ala Thr Leu Thr Ala Asp Lys Ser Ser Ser 
                85                  90                  95      


Thr Ala Tyr Met Glu Leu Arg Ser Leu Thr Ser Glu Asp Ser Ala Val 
            100                 105                 110         


Tyr Tyr Cys Thr Arg Phe Tyr Ser Tyr Thr Tyr Trp Gly Gln Gly Thr 
        115                 120                 125             


Leu Val Thr Val Ser Ala Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser 
    130                 135                 140                 


Gly Gly Gly Gly Ser Asp Val Val Met Thr Gln Thr Pro Leu Ser Leu 
145                 150                 155                 160 


Pro Val Ser Leu Gly Asp Gln Ala Ser Ile Ser Cys Arg Ser Ser Gln 
                165                 170                 175     


Ser Leu Val His Ser Asn Gly Asn Thr Tyr Leu His Trp Tyr Leu Gln 
            180                 185                 190         


Lys Pro Gly Gln Ser Pro Lys Leu Leu Ile Tyr Lys Val Ser Asn Arg 
        195                 200                 205             


Phe Ser Gly Val Pro Asp Arg Phe Ser Gly Ser Gly Ser Gly Thr Asp 
    210                 215                 220                 


Phe Thr Leu Lys Ile Ser Arg Val Glu Ala Glu Asp Leu Gly Val Tyr 
225                 230                 235                 240 


Phe Cys Ser Gln Asn Thr His Val Pro Pro Thr Phe Gly Ser Gly Thr 
                245                 250                 255     


Lys Leu Glu Ile Lys Glu Pro Lys Ser Cys Asp Lys Thr His Thr Cys 
            260                 265                 270         


Pro Pro Cys Pro Asp Pro Lys Ile Tyr Ile Trp Ala Pro Leu Ala Gly 
        275                 280                 285             


Thr Cys Gly Val Leu Leu Leu Ser Leu Val Ile Thr Leu Tyr Cys Asn 
    290                 295                 300                 


His Arg Asn Ile Val Ile Phe Leu Asn Arg Arg Arg Arg Arg Glu Arg 
305                 310                 315                 320 


Arg Asp Leu Phe Thr Glu Ser Trp Asp Thr Gln Lys Ala Pro Asn Asn 
                325                 330                 335     


Tyr Arg Ser Pro Ile Ser Thr Ser Gln Pro Thr Asn Gln Ser Met Asp 
            340                 345                 350         


Asp Thr Arg Glu Asp Ile Tyr Val Asn Tyr Pro Thr Phe Ser Arg Arg 
        355                 360                 365             


Pro Lys Thr Arg Val Phe Trp Val Arg Ser Lys Arg Ser Arg Leu Leu 
    370                 375                 380                 


His Ser Asp Tyr Met Asn Met Thr Pro Arg Arg Pro Gly Pro Thr Arg 
385                 390                 395                 400 


Lys His Tyr Gln Pro Tyr Ala Pro Pro Arg Asp Phe Ala Ala Tyr Arg 
                405                 410                 415     


Ser Arg Val Lys Phe Ser Arg Ser Ala Asp Ala Pro Ala Tyr Gln Gln 
            420                 425                 430         


Gly Gln Asn Gln Leu Tyr Asn Glu Leu Asn Leu Gly Arg Arg Glu Glu 
        435                 440                 445             


Tyr Asp Val Leu Asp Lys Arg Arg Gly Arg Asp Pro Glu Met Gly Gly 
    450                 455                 460                 


Lys Pro Arg Arg Lys Asn Pro Gln Glu Gly Leu Tyr Asn Glu Leu Gln 
465                 470                 475                 480 


Lys Asp Lys Met Ala Glu Ala Tyr Ser Glu Ile Gly Met Lys Gly Glu 
                485                 490                 495     


Arg Arg Arg Gly Lys Gly His Asp Gly Leu Tyr Gln Gly Leu Ser Thr 
            500                 505                 510         


Ala Thr Lys Asp Thr Tyr Asp Ala Leu His Met Gln Ala Leu Pro Pro 
        515                 520                 525             


Arg 
    


<210>  33
<211>  636
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  hIgG heavy chain signal sequence/scFV GC33/hIgG1 hinge/CD8alpha 
       TMD/F36V-FKBP/CD226/CD28/CD3zeta

<400>  33

Met Asp Trp Ile Trp Arg Ile Leu Phe Leu Val Gly Ala Ala Thr Gly 
1               5                   10                  15      


Ala His Ser Gln Val Gln Leu Gln Gln Ser Gly Ala Glu Leu Val Arg 
            20                  25                  30          


Pro Gly Ala Ser Val Lys Leu Ser Cys Lys Ala Ser Gly Tyr Thr Phe 
        35                  40                  45              


Thr Asp Tyr Glu Met His Trp Val Lys Gln Thr Pro Val His Gly Leu 
    50                  55                  60                  


Lys Trp Ile Gly Ala Leu Asp Pro Lys Thr Gly Asp Thr Ala Tyr Ser 
65                  70                  75                  80  


Gln Lys Phe Lys Gly Lys Ala Thr Leu Thr Ala Asp Lys Ser Ser Ser 
                85                  90                  95      


Thr Ala Tyr Met Glu Leu Arg Ser Leu Thr Ser Glu Asp Ser Ala Val 
            100                 105                 110         


Tyr Tyr Cys Thr Arg Phe Tyr Ser Tyr Thr Tyr Trp Gly Gln Gly Thr 
        115                 120                 125             


Leu Val Thr Val Ser Ala Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser 
    130                 135                 140                 


Gly Gly Gly Gly Ser Asp Val Val Met Thr Gln Thr Pro Leu Ser Leu 
145                 150                 155                 160 


Pro Val Ser Leu Gly Asp Gln Ala Ser Ile Ser Cys Arg Ser Ser Gln 
                165                 170                 175     


Ser Leu Val His Ser Asn Gly Asn Thr Tyr Leu His Trp Tyr Leu Gln 
            180                 185                 190         


Lys Pro Gly Gln Ser Pro Lys Leu Leu Ile Tyr Lys Val Ser Asn Arg 
        195                 200                 205             


Phe Ser Gly Val Pro Asp Arg Phe Ser Gly Ser Gly Ser Gly Thr Asp 
    210                 215                 220                 


Phe Thr Leu Lys Ile Ser Arg Val Glu Ala Glu Asp Leu Gly Val Tyr 
225                 230                 235                 240 


Phe Cys Ser Gln Asn Thr His Val Pro Pro Thr Phe Gly Ser Gly Thr 
                245                 250                 255     


Lys Leu Glu Ile Lys Glu Pro Lys Ser Cys Asp Lys Thr His Thr Cys 
            260                 265                 270         


Pro Pro Cys Pro Asp Pro Lys Ile Tyr Ile Trp Ala Pro Leu Ala Gly 
        275                 280                 285             


Thr Cys Gly Val Leu Leu Leu Ser Leu Val Ile Thr Leu Tyr Cys Asn 
    290                 295                 300                 


His Arg Asn Gly Val Gln Val Glu Thr Ile Ser Pro Gly Asp Gly Arg 
305                 310                 315                 320 


Thr Phe Pro Lys Arg Gly Gln Thr Cys Val Val His Tyr Thr Gly Met 
                325                 330                 335     


Leu Glu Asp Gly Lys Lys Val Asp Ser Ser Arg Asp Arg Asn Lys Pro 
            340                 345                 350         


Phe Lys Phe Met Leu Gly Lys Gln Glu Val Ile Arg Gly Trp Glu Glu 
        355                 360                 365             


Gly Val Ala Gln Met Ser Val Gly Gln Arg Ala Lys Leu Thr Ile Ser 
    370                 375                 380                 


Pro Asp Tyr Ala Tyr Gly Ala Thr Gly His Pro Gly Ile Ile Pro Pro 
385                 390                 395                 400 


His Ala Thr Leu Val Phe Asp Val Glu Leu Leu Lys Leu Glu Ile Val 
                405                 410                 415     


Ile Phe Leu Asn Arg Arg Arg Arg Arg Glu Arg Arg Asp Leu Phe Thr 
            420                 425                 430         


Glu Ser Trp Asp Thr Gln Lys Ala Pro Asn Asn Tyr Arg Ser Pro Ile 
        435                 440                 445             


Ser Thr Ser Gln Pro Thr Asn Gln Ser Met Asp Asp Thr Arg Glu Asp 
    450                 455                 460                 


Ile Tyr Val Asn Tyr Pro Thr Phe Ser Arg Arg Pro Lys Thr Arg Val 
465                 470                 475                 480 


Phe Trp Val Arg Ser Lys Arg Ser Arg Leu Leu His Ser Asp Tyr Met 
                485                 490                 495     


Asn Met Thr Pro Arg Arg Pro Gly Pro Thr Arg Lys His Tyr Gln Pro 
            500                 505                 510         


Tyr Ala Pro Pro Arg Asp Phe Ala Ala Tyr Arg Ser Arg Val Lys Phe 
        515                 520                 525             


Ser Arg Ser Ala Asp Ala Pro Ala Tyr Gln Gln Gly Gln Asn Gln Leu 
    530                 535                 540                 


Tyr Asn Glu Leu Asn Leu Gly Arg Arg Glu Glu Tyr Asp Val Leu Asp 
545                 550                 555                 560 


Lys Arg Arg Gly Arg Asp Pro Glu Met Gly Gly Lys Pro Arg Arg Lys 
                565                 570                 575     


Asn Pro Gln Glu Gly Leu Tyr Asn Glu Leu Gln Lys Asp Lys Met Ala 
            580                 585                 590         


Glu Ala Tyr Ser Glu Ile Gly Met Lys Gly Glu Arg Arg Arg Gly Lys 
        595                 600                 605             


Gly His Asp Gly Leu Tyr Gln Gly Leu Ser Thr Ala Thr Lys Asp Thr 
    610                 615                 620                 


Tyr Asp Ala Leu His Met Gln Ala Leu Pro Pro Arg 
625                 630                 635     


<210>  34
<211>  527
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  hIgG heavy chain signal sequence/scFV GC33/hIgG1 hinge/CD8alpha 
       TMD/CD226/4-1BB/CD3zeta

<400>  34

Met Asp Trp Ile Trp Arg Ile Leu Phe Leu Val Gly Ala Ala Thr Gly 
1               5                   10                  15      


Ala His Ser Gln Val Gln Leu Gln Gln Ser Gly Ala Glu Leu Val Arg 
            20                  25                  30          


Pro Gly Ala Ser Val Lys Leu Ser Cys Lys Ala Ser Gly Tyr Thr Phe 
        35                  40                  45              


Thr Asp Tyr Glu Met His Trp Val Lys Gln Thr Pro Val His Gly Leu 
    50                  55                  60                  


Lys Trp Ile Gly Ala Leu Asp Pro Lys Thr Gly Asp Thr Ala Tyr Ser 
65                  70                  75                  80  


Gln Lys Phe Lys Gly Lys Ala Thr Leu Thr Ala Asp Lys Ser Ser Ser 
                85                  90                  95      


Thr Ala Tyr Met Glu Leu Arg Ser Leu Thr Ser Glu Asp Ser Ala Val 
            100                 105                 110         


Tyr Tyr Cys Thr Arg Phe Tyr Ser Tyr Thr Tyr Trp Gly Gln Gly Thr 
        115                 120                 125             


Leu Val Thr Val Ser Ala Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser 
    130                 135                 140                 


Gly Gly Gly Gly Ser Asp Val Val Met Thr Gln Thr Pro Leu Ser Leu 
145                 150                 155                 160 


Pro Val Ser Leu Gly Asp Gln Ala Ser Ile Ser Cys Arg Ser Ser Gln 
                165                 170                 175     


Ser Leu Val His Ser Asn Gly Asn Thr Tyr Leu His Trp Tyr Leu Gln 
            180                 185                 190         


Lys Pro Gly Gln Ser Pro Lys Leu Leu Ile Tyr Lys Val Ser Asn Arg 
        195                 200                 205             


Phe Ser Gly Val Pro Asp Arg Phe Ser Gly Ser Gly Ser Gly Thr Asp 
    210                 215                 220                 


Phe Thr Leu Lys Ile Ser Arg Val Glu Ala Glu Asp Leu Gly Val Tyr 
225                 230                 235                 240 


Phe Cys Ser Gln Asn Thr His Val Pro Pro Thr Phe Gly Ser Gly Thr 
                245                 250                 255     


Lys Leu Glu Ile Lys Glu Pro Lys Ser Cys Asp Lys Thr His Thr Cys 
            260                 265                 270         


Pro Pro Cys Pro Asp Pro Lys Ile Tyr Ile Trp Ala Pro Leu Ala Gly 
        275                 280                 285             


Thr Cys Gly Val Leu Leu Leu Ser Leu Val Ile Thr Leu Tyr Cys Asn 
    290                 295                 300                 


His Arg Asn Ile Val Ile Phe Leu Asn Arg Arg Arg Arg Arg Glu Arg 
305                 310                 315                 320 


Arg Asp Leu Phe Thr Glu Ser Trp Asp Thr Gln Lys Ala Pro Asn Asn 
                325                 330                 335     


Tyr Arg Ser Pro Ile Ser Thr Ser Gln Pro Thr Asn Gln Ser Met Asp 
            340                 345                 350         


Asp Thr Arg Glu Asp Ile Tyr Val Asn Tyr Pro Thr Phe Ser Arg Arg 
        355                 360                 365             


Pro Lys Thr Arg Val Lys Arg Gly Arg Lys Lys Leu Leu Tyr Ile Phe 
    370                 375                 380                 


Lys Gln Pro Phe Met Arg Pro Val Gln Thr Thr Gln Glu Glu Asp Gly 
385                 390                 395                 400 


Cys Ser Cys Arg Phe Pro Glu Glu Glu Glu Gly Gly Cys Glu Leu Arg 
                405                 410                 415     


Val Lys Phe Ser Arg Ser Ala Asp Ala Pro Ala Tyr Gln Gln Gly Gln 
            420                 425                 430         


Asn Gln Leu Tyr Asn Glu Leu Asn Leu Gly Arg Arg Glu Glu Tyr Asp 
        435                 440                 445             


Val Leu Asp Lys Arg Arg Gly Arg Asp Pro Glu Met Gly Gly Lys Pro 
    450                 455                 460                 


Arg Arg Lys Asn Pro Gln Glu Gly Leu Tyr Asn Glu Leu Gln Lys Asp 
465                 470                 475                 480 


Lys Met Ala Glu Ala Tyr Ser Glu Ile Gly Met Lys Gly Glu Arg Arg 
                485                 490                 495     


Arg Gly Lys Gly His Asp Gly Leu Tyr Gln Gly Leu Ser Thr Ala Thr 
            500                 505                 510         


Lys Asp Thr Tyr Asp Ala Leu His Met Gln Ala Leu Pro Pro Arg 
        515                 520                 525         


<210>  35
<211>  634
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  hIgG heavy chain signal sequence/scFV GC33/hIgG1 hinge/CD8alpha 
       TMD/F36V-FKBP/CD226/41BB/CD3zeta

<400>  35

Met Asp Trp Ile Trp Arg Ile Leu Phe Leu Val Gly Ala Ala Thr Gly 
1               5                   10                  15      


Ala His Ser Gln Val Gln Leu Gln Gln Ser Gly Ala Glu Leu Val Arg 
            20                  25                  30          


Pro Gly Ala Ser Val Lys Leu Ser Cys Lys Ala Ser Gly Tyr Thr Phe 
        35                  40                  45              


Thr Asp Tyr Glu Met His Trp Val Lys Gln Thr Pro Val His Gly Leu 
    50                  55                  60                  


Lys Trp Ile Gly Ala Leu Asp Pro Lys Thr Gly Asp Thr Ala Tyr Ser 
65                  70                  75                  80  


Gln Lys Phe Lys Gly Lys Ala Thr Leu Thr Ala Asp Lys Ser Ser Ser 
                85                  90                  95      


Thr Ala Tyr Met Glu Leu Arg Ser Leu Thr Ser Glu Asp Ser Ala Val 
            100                 105                 110         


Tyr Tyr Cys Thr Arg Phe Tyr Ser Tyr Thr Tyr Trp Gly Gln Gly Thr 
        115                 120                 125             


Leu Val Thr Val Ser Ala Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser 
    130                 135                 140                 


Gly Gly Gly Gly Ser Asp Val Val Met Thr Gln Thr Pro Leu Ser Leu 
145                 150                 155                 160 


Pro Val Ser Leu Gly Asp Gln Ala Ser Ile Ser Cys Arg Ser Ser Gln 
                165                 170                 175     


Ser Leu Val His Ser Asn Gly Asn Thr Tyr Leu His Trp Tyr Leu Gln 
            180                 185                 190         


Lys Pro Gly Gln Ser Pro Lys Leu Leu Ile Tyr Lys Val Ser Asn Arg 
        195                 200                 205             


Phe Ser Gly Val Pro Asp Arg Phe Ser Gly Ser Gly Ser Gly Thr Asp 
    210                 215                 220                 


Phe Thr Leu Lys Ile Ser Arg Val Glu Ala Glu Asp Leu Gly Val Tyr 
225                 230                 235                 240 


Phe Cys Ser Gln Asn Thr His Val Pro Pro Thr Phe Gly Ser Gly Thr 
                245                 250                 255     


Lys Leu Glu Ile Lys Glu Pro Lys Ser Cys Asp Lys Thr His Thr Cys 
            260                 265                 270         


Pro Pro Cys Pro Asp Pro Lys Ile Tyr Ile Trp Ala Pro Leu Ala Gly 
        275                 280                 285             


Thr Cys Gly Val Leu Leu Leu Ser Leu Val Ile Thr Leu Tyr Cys Asn 
    290                 295                 300                 


His Arg Asn Gly Val Gln Val Glu Thr Ile Ser Pro Gly Asp Gly Arg 
305                 310                 315                 320 


Thr Phe Pro Lys Arg Gly Gln Thr Cys Val Val His Tyr Thr Gly Met 
                325                 330                 335     


Leu Glu Asp Gly Lys Lys Val Asp Ser Ser Arg Asp Arg Asn Lys Pro 
            340                 345                 350         


Phe Lys Phe Met Leu Gly Lys Gln Glu Val Ile Arg Gly Trp Glu Glu 
        355                 360                 365             


Gly Val Ala Gln Met Ser Val Gly Gln Arg Ala Lys Leu Thr Ile Ser 
    370                 375                 380                 


Pro Asp Tyr Ala Tyr Gly Ala Thr Gly His Pro Gly Ile Ile Pro Pro 
385                 390                 395                 400 


His Ala Thr Leu Val Phe Asp Val Glu Leu Leu Lys Leu Glu Ile Val 
                405                 410                 415     


Ile Phe Leu Asn Arg Arg Arg Arg Arg Glu Arg Arg Asp Leu Phe Thr 
            420                 425                 430         


Glu Ser Trp Asp Thr Gln Lys Ala Pro Asn Asn Tyr Arg Ser Pro Ile 
        435                 440                 445             


Ser Thr Ser Gln Pro Thr Asn Gln Ser Met Asp Asp Thr Arg Glu Asp 
    450                 455                 460                 


Ile Tyr Val Asn Tyr Pro Thr Phe Ser Arg Arg Pro Lys Thr Arg Val 
465                 470                 475                 480 


Lys Arg Gly Arg Lys Lys Leu Leu Tyr Ile Phe Lys Gln Pro Phe Met 
                485                 490                 495     


Arg Pro Val Gln Thr Thr Gln Glu Glu Asp Gly Cys Ser Cys Arg Phe 
            500                 505                 510         


Pro Glu Glu Glu Glu Gly Gly Cys Glu Leu Arg Val Lys Phe Ser Arg 
        515                 520                 525             


Ser Ala Asp Ala Pro Ala Tyr Gln Gln Gly Gln Asn Gln Leu Tyr Asn 
    530                 535                 540                 


Glu Leu Asn Leu Gly Arg Arg Glu Glu Tyr Asp Val Leu Asp Lys Arg 
545                 550                 555                 560 


Arg Gly Arg Asp Pro Glu Met Gly Gly Lys Pro Arg Arg Lys Asn Pro 
                565                 570                 575     


Gln Glu Gly Leu Tyr Asn Glu Leu Gln Lys Asp Lys Met Ala Glu Ala 
            580                 585                 590         


Tyr Ser Glu Ile Gly Met Lys Gly Glu Arg Arg Arg Gly Lys Gly His 
        595                 600                 605             


Asp Gly Leu Tyr Gln Gly Leu Ser Thr Ala Thr Lys Asp Thr Tyr Asp 
    610                 615                 620                 


Ala Leu His Met Gln Ala Leu Pro Pro Arg 
625                 630                 


<210>  36
<211>  571
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  hIgG heavy chain signal sequence/scFV GC33/hIgG1 hinge/CD8alpha 
       TMD/CD226/CD28/4-1BB/CD3zeta

<400>  36

Met Asp Trp Ile Trp Arg Ile Leu Phe Leu Val Gly Ala Ala Thr Gly 
1               5                   10                  15      


Ala His Ser Gln Val Gln Leu Gln Gln Ser Gly Ala Glu Leu Val Arg 
            20                  25                  30          


Pro Gly Ala Ser Val Lys Leu Ser Cys Lys Ala Ser Gly Tyr Thr Phe 
        35                  40                  45              


Thr Asp Tyr Glu Met His Trp Val Lys Gln Thr Pro Val His Gly Leu 
    50                  55                  60                  


Lys Trp Ile Gly Ala Leu Asp Pro Lys Thr Gly Asp Thr Ala Tyr Ser 
65                  70                  75                  80  


Gln Lys Phe Lys Gly Lys Ala Thr Leu Thr Ala Asp Lys Ser Ser Ser 
                85                  90                  95      


Thr Ala Tyr Met Glu Leu Arg Ser Leu Thr Ser Glu Asp Ser Ala Val 
            100                 105                 110         


Tyr Tyr Cys Thr Arg Phe Tyr Ser Tyr Thr Tyr Trp Gly Gln Gly Thr 
        115                 120                 125             


Leu Val Thr Val Ser Ala Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser 
    130                 135                 140                 


Gly Gly Gly Gly Ser Asp Val Val Met Thr Gln Thr Pro Leu Ser Leu 
145                 150                 155                 160 


Pro Val Ser Leu Gly Asp Gln Ala Ser Ile Ser Cys Arg Ser Ser Gln 
                165                 170                 175     


Ser Leu Val His Ser Asn Gly Asn Thr Tyr Leu His Trp Tyr Leu Gln 
            180                 185                 190         


Lys Pro Gly Gln Ser Pro Lys Leu Leu Ile Tyr Lys Val Ser Asn Arg 
        195                 200                 205             


Phe Ser Gly Val Pro Asp Arg Phe Ser Gly Ser Gly Ser Gly Thr Asp 
    210                 215                 220                 


Phe Thr Leu Lys Ile Ser Arg Val Glu Ala Glu Asp Leu Gly Val Tyr 
225                 230                 235                 240 


Phe Cys Ser Gln Asn Thr His Val Pro Pro Thr Phe Gly Ser Gly Thr 
                245                 250                 255     


Lys Leu Glu Ile Lys Glu Pro Lys Ser Cys Asp Lys Thr His Thr Cys 
            260                 265                 270         


Pro Pro Cys Pro Asp Pro Lys Ile Tyr Ile Trp Ala Pro Leu Ala Gly 
        275                 280                 285             


Thr Cys Gly Val Leu Leu Leu Ser Leu Val Ile Thr Leu Tyr Cys Asn 
    290                 295                 300                 


His Arg Asn Ile Val Ile Phe Leu Asn Arg Arg Arg Arg Arg Glu Arg 
305                 310                 315                 320 


Arg Asp Leu Phe Thr Glu Ser Trp Asp Thr Gln Lys Ala Pro Asn Asn 
                325                 330                 335     


Tyr Arg Ser Pro Ile Ser Thr Ser Gln Pro Thr Asn Gln Ser Met Asp 
            340                 345                 350         


Asp Thr Arg Glu Asp Ile Tyr Val Asn Tyr Pro Thr Phe Ser Arg Arg 
        355                 360                 365             


Pro Lys Thr Arg Val Phe Trp Val Arg Ser Lys Arg Ser Arg Leu Leu 
    370                 375                 380                 


His Ser Asp Tyr Met Asn Met Thr Pro Arg Arg Pro Gly Pro Thr Arg 
385                 390                 395                 400 


Lys His Tyr Gln Pro Tyr Ala Pro Pro Arg Asp Phe Ala Ala Tyr Arg 
                405                 410                 415     


Ser Lys Arg Gly Arg Lys Lys Leu Leu Tyr Ile Phe Lys Gln Pro Phe 
            420                 425                 430         


Met Arg Pro Val Gln Thr Thr Gln Glu Glu Asp Gly Cys Ser Cys Arg 
        435                 440                 445             


Phe Pro Glu Glu Glu Glu Gly Gly Cys Glu Leu Arg Val Lys Phe Ser 
    450                 455                 460                 


Arg Ser Ala Asp Ala Pro Ala Tyr Gln Gln Gly Gln Asn Gln Leu Tyr 
465                 470                 475                 480 


Asn Glu Leu Asn Leu Gly Arg Arg Glu Glu Tyr Asp Val Leu Asp Lys 
                485                 490                 495     


Arg Arg Gly Arg Asp Pro Glu Met Gly Gly Lys Pro Arg Arg Lys Asn 
            500                 505                 510         


Pro Gln Glu Gly Leu Tyr Asn Glu Leu Gln Lys Asp Lys Met Ala Glu 
        515                 520                 525             


Ala Tyr Ser Glu Ile Gly Met Lys Gly Glu Arg Arg Arg Gly Lys Gly 
    530                 535                 540                 


His Asp Gly Leu Tyr Gln Gly Leu Ser Thr Ala Thr Lys Asp Thr Tyr 
545                 550                 555                 560 


Asp Ala Leu His Met Gln Ala Leu Pro Pro Arg 
                565                 570     


<210>  37
<211>  678
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  hIgG heavy chain signal sequence/scFV GC33/hIgG1 hinge/CD8alpha 
       TMD/F36V-FKBP/CD226/CD28/41BB/CD3zeta

<400>  37

Met Asp Trp Ile Trp Arg Ile Leu Phe Leu Val Gly Ala Ala Thr Gly 
1               5                   10                  15      


Ala His Ser Gln Val Gln Leu Gln Gln Ser Gly Ala Glu Leu Val Arg 
            20                  25                  30          


Pro Gly Ala Ser Val Lys Leu Ser Cys Lys Ala Ser Gly Tyr Thr Phe 
        35                  40                  45              


Thr Asp Tyr Glu Met His Trp Val Lys Gln Thr Pro Val His Gly Leu 
    50                  55                  60                  


Lys Trp Ile Gly Ala Leu Asp Pro Lys Thr Gly Asp Thr Ala Tyr Ser 
65                  70                  75                  80  


Gln Lys Phe Lys Gly Lys Ala Thr Leu Thr Ala Asp Lys Ser Ser Ser 
                85                  90                  95      


Thr Ala Tyr Met Glu Leu Arg Ser Leu Thr Ser Glu Asp Ser Ala Val 
            100                 105                 110         


Tyr Tyr Cys Thr Arg Phe Tyr Ser Tyr Thr Tyr Trp Gly Gln Gly Thr 
        115                 120                 125             


Leu Val Thr Val Ser Ala Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser 
    130                 135                 140                 


Gly Gly Gly Gly Ser Asp Val Val Met Thr Gln Thr Pro Leu Ser Leu 
145                 150                 155                 160 


Pro Val Ser Leu Gly Asp Gln Ala Ser Ile Ser Cys Arg Ser Ser Gln 
                165                 170                 175     


Ser Leu Val His Ser Asn Gly Asn Thr Tyr Leu His Trp Tyr Leu Gln 
            180                 185                 190         


Lys Pro Gly Gln Ser Pro Lys Leu Leu Ile Tyr Lys Val Ser Asn Arg 
        195                 200                 205             


Phe Ser Gly Val Pro Asp Arg Phe Ser Gly Ser Gly Ser Gly Thr Asp 
    210                 215                 220                 


Phe Thr Leu Lys Ile Ser Arg Val Glu Ala Glu Asp Leu Gly Val Tyr 
225                 230                 235                 240 


Phe Cys Ser Gln Asn Thr His Val Pro Pro Thr Phe Gly Ser Gly Thr 
                245                 250                 255     


Lys Leu Glu Ile Lys Glu Pro Lys Ser Cys Asp Lys Thr His Thr Cys 
            260                 265                 270         


Pro Pro Cys Pro Asp Pro Lys Ile Tyr Ile Trp Ala Pro Leu Ala Gly 
        275                 280                 285             


Thr Cys Gly Val Leu Leu Leu Ser Leu Val Ile Thr Leu Tyr Cys Asn 
    290                 295                 300                 


His Arg Asn Gly Val Gln Val Glu Thr Ile Ser Pro Gly Asp Gly Arg 
305                 310                 315                 320 


Thr Phe Pro Lys Arg Gly Gln Thr Cys Val Val His Tyr Thr Gly Met 
                325                 330                 335     


Leu Glu Asp Gly Lys Lys Val Asp Ser Ser Arg Asp Arg Asn Lys Pro 
            340                 345                 350         


Phe Lys Phe Met Leu Gly Lys Gln Glu Val Ile Arg Gly Trp Glu Glu 
        355                 360                 365             


Gly Val Ala Gln Met Ser Val Gly Gln Arg Ala Lys Leu Thr Ile Ser 
    370                 375                 380                 


Pro Asp Tyr Ala Tyr Gly Ala Thr Gly His Pro Gly Ile Ile Pro Pro 
385                 390                 395                 400 


His Ala Thr Leu Val Phe Asp Val Glu Leu Leu Lys Leu Glu Ile Val 
                405                 410                 415     


Ile Phe Leu Asn Arg Arg Arg Arg Arg Glu Arg Arg Asp Leu Phe Thr 
            420                 425                 430         


Glu Ser Trp Asp Thr Gln Lys Ala Pro Asn Asn Tyr Arg Ser Pro Ile 
        435                 440                 445             


Ser Thr Ser Gln Pro Thr Asn Gln Ser Met Asp Asp Thr Arg Glu Asp 
    450                 455                 460                 


Ile Tyr Val Asn Tyr Pro Thr Phe Ser Arg Arg Pro Lys Thr Arg Val 
465                 470                 475                 480 


Phe Trp Val Arg Ser Lys Arg Ser Arg Leu Leu His Ser Asp Tyr Met 
                485                 490                 495     


Asn Met Thr Pro Arg Arg Pro Gly Pro Thr Arg Lys His Tyr Gln Pro 
            500                 505                 510         


Tyr Ala Pro Pro Arg Asp Phe Ala Ala Tyr Arg Ser Lys Arg Gly Arg 
        515                 520                 525             


Lys Lys Leu Leu Tyr Ile Phe Lys Gln Pro Phe Met Arg Pro Val Gln 
    530                 535                 540                 


Thr Thr Gln Glu Glu Asp Gly Cys Ser Cys Arg Phe Pro Glu Glu Glu 
545                 550                 555                 560 


Glu Gly Gly Cys Glu Leu Arg Val Lys Phe Ser Arg Ser Ala Asp Ala 
                565                 570                 575     


Pro Ala Tyr Gln Gln Gly Gln Asn Gln Leu Tyr Asn Glu Leu Asn Leu 
            580                 585                 590         


Gly Arg Arg Glu Glu Tyr Asp Val Leu Asp Lys Arg Arg Gly Arg Asp 
        595                 600                 605             


Pro Glu Met Gly Gly Lys Pro Arg Arg Lys Asn Pro Gln Glu Gly Leu 
    610                 615                 620                 


Tyr Asn Glu Leu Gln Lys Asp Lys Met Ala Glu Ala Tyr Ser Glu Ile 
625                 630                 635                 640 


Gly Met Lys Gly Glu Arg Arg Arg Gly Lys Gly His Asp Gly Leu Tyr 
                645                 650                 655     


Gln Gly Leu Ser Thr Ala Thr Lys Asp Thr Tyr Asp Ala Leu His Met 
            660                 665                 670         


Gln Ala Leu Pro Pro Arg 
        675             


<210>  38
<211>  545
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  scFV GC33/hIgG1 hinge/CD28 TMD/F36V-FKBP/41BB/CD3zeta

<400>  38

Gln Val Gln Leu Gln Gln Ser Gly Ala Glu Leu Val Arg Pro Gly Ala 
1               5                   10                  15      


Ser Val Lys Leu Ser Cys Lys Ala Ser Gly Tyr Thr Phe Thr Asp Tyr 
            20                  25                  30          


Glu Met His Trp Val Lys Gln Thr Pro Val His Gly Leu Lys Trp Ile 
        35                  40                  45              


Gly Ala Leu Asp Pro Lys Thr Gly Asp Thr Ala Tyr Ser Gln Lys Phe 
    50                  55                  60                  


Lys Gly Lys Ala Thr Leu Thr Ala Asp Lys Ser Ser Ser Thr Ala Tyr 
65                  70                  75                  80  


Met Glu Leu Arg Ser Leu Thr Ser Glu Asp Ser Ala Val Tyr Tyr Cys 
                85                  90                  95      


Thr Arg Phe Tyr Ser Tyr Thr Tyr Trp Gly Gln Gly Thr Leu Val Thr 
            100                 105                 110         


Val Ser Ala Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly 
        115                 120                 125             


Gly Ser Asp Val Val Met Thr Gln Thr Pro Leu Ser Leu Pro Val Ser 
    130                 135                 140                 


Leu Gly Asp Gln Ala Ser Ile Ser Cys Arg Ser Ser Gln Ser Leu Val 
145                 150                 155                 160 


His Ser Asn Gly Asn Thr Tyr Leu His Trp Tyr Leu Gln Lys Pro Gly 
                165                 170                 175     


Gln Ser Pro Lys Leu Leu Ile Tyr Lys Val Ser Asn Arg Phe Ser Gly 
            180                 185                 190         


Val Pro Asp Arg Phe Ser Gly Ser Gly Ser Gly Thr Asp Phe Thr Leu 
        195                 200                 205             


Lys Ile Ser Arg Val Glu Ala Glu Asp Leu Gly Val Tyr Phe Cys Ser 
    210                 215                 220                 


Gln Asn Thr His Val Pro Pro Thr Phe Gly Ser Gly Thr Lys Leu Glu 
225                 230                 235                 240 


Ile Lys Glu Pro Lys Ser Cys Asp Lys Thr His Thr Cys Pro Pro Cys 
                245                 250                 255     


Pro Asp Pro Lys Phe Trp Val Leu Val Val Val Gly Gly Val Leu Ala 
            260                 265                 270         


Cys Tyr Ser Leu Leu Val Thr Val Ala Phe Ile Ile Gly Val Gln Val 
        275                 280                 285             


Glu Thr Ile Ser Pro Gly Asp Gly Arg Thr Phe Pro Lys Arg Gly Gln 
    290                 295                 300                 


Thr Cys Val Val His Tyr Thr Gly Met Leu Glu Asp Gly Lys Lys Val 
305                 310                 315                 320 


Asp Ser Ser Arg Asp Arg Asn Lys Pro Phe Lys Phe Met Leu Gly Lys 
                325                 330                 335     


Gln Glu Val Ile Arg Gly Trp Glu Glu Gly Val Ala Gln Met Ser Val 
            340                 345                 350         


Gly Gln Arg Ala Lys Leu Thr Ile Ser Pro Asp Tyr Ala Tyr Gly Ala 
        355                 360                 365             


Thr Gly His Pro Gly Ile Ile Pro Pro His Ala Thr Leu Val Phe Asp 
    370                 375                 380                 


Val Glu Leu Leu Lys Leu Glu Lys Arg Gly Arg Lys Lys Leu Leu Tyr 
385                 390                 395                 400 


Ile Phe Lys Gln Pro Phe Met Arg Pro Val Gln Thr Thr Gln Glu Glu 
                405                 410                 415     


Asp Gly Cys Ser Cys Arg Phe Pro Glu Glu Glu Glu Gly Gly Cys Glu 
            420                 425                 430         


Leu Arg Val Lys Phe Ser Arg Ser Ala Asp Ala Pro Ala Tyr Gln Gln 
        435                 440                 445             


Gly Gln Asn Gln Leu Tyr Asn Glu Leu Asn Leu Gly Arg Arg Glu Glu 
    450                 455                 460                 


Tyr Asp Val Leu Asp Lys Arg Arg Gly Arg Asp Pro Glu Met Gly Gly 
465                 470                 475                 480 


Lys Pro Arg Arg Lys Asn Pro Gln Glu Gly Leu Tyr Asn Glu Leu Gln 
                485                 490                 495     


Lys Asp Lys Met Ala Glu Ala Tyr Ser Glu Ile Gly Met Lys Gly Glu 
            500                 505                 510         


Arg Arg Arg Gly Lys Gly His Asp Gly Leu Tyr Gln Gly Leu Ser Thr 
        515                 520                 525             


Ala Thr Lys Asp Thr Tyr Asp Ala Leu His Met Gln Ala Leu Pro Pro 
    530                 535                 540                 


Arg 
545 


<210>  39
<211>  442
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  scFV GC33/hIgG1 hinge/CD8alpha TMD/41BB/CD3zeta

<400>  39

Gln Val Gln Leu Gln Gln Ser Gly Ala Glu Leu Val Arg Pro Gly Ala 
1               5                   10                  15      


Ser Val Lys Leu Ser Cys Lys Ala Ser Gly Tyr Thr Phe Thr Asp Tyr 
            20                  25                  30          


Glu Met His Trp Val Lys Gln Thr Pro Val His Gly Leu Lys Trp Ile 
        35                  40                  45              


Gly Ala Leu Asp Pro Lys Thr Gly Asp Thr Ala Tyr Ser Gln Lys Phe 
    50                  55                  60                  


Lys Gly Lys Ala Thr Leu Thr Ala Asp Lys Ser Ser Ser Thr Ala Tyr 
65                  70                  75                  80  


Met Glu Leu Arg Ser Leu Thr Ser Glu Asp Ser Ala Val Tyr Tyr Cys 
                85                  90                  95      


Thr Arg Phe Tyr Ser Tyr Thr Tyr Trp Gly Gln Gly Thr Leu Val Thr 
            100                 105                 110         


Val Ser Ala Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly 
        115                 120                 125             


Gly Ser Asp Val Val Met Thr Gln Thr Pro Leu Ser Leu Pro Val Ser 
    130                 135                 140                 


Leu Gly Asp Gln Ala Ser Ile Ser Cys Arg Ser Ser Gln Ser Leu Val 
145                 150                 155                 160 


His Ser Asn Gly Asn Thr Tyr Leu His Trp Tyr Leu Gln Lys Pro Gly 
                165                 170                 175     


Gln Ser Pro Lys Leu Leu Ile Tyr Lys Val Ser Asn Arg Phe Ser Gly 
            180                 185                 190         


Val Pro Asp Arg Phe Ser Gly Ser Gly Ser Gly Thr Asp Phe Thr Leu 
        195                 200                 205             


Lys Ile Ser Arg Val Glu Ala Glu Asp Leu Gly Val Tyr Phe Cys Ser 
    210                 215                 220                 


Gln Asn Thr His Val Pro Pro Thr Phe Gly Ser Gly Thr Lys Leu Glu 
225                 230                 235                 240 


Ile Lys Glu Pro Lys Ser Cys Asp Lys Thr His Thr Cys Pro Pro Cys 
                245                 250                 255     


Pro Asp Pro Lys Ile Tyr Ile Trp Ala Pro Leu Ala Gly Thr Cys Gly 
            260                 265                 270         


Val Leu Leu Leu Ser Leu Val Ile Thr Leu Tyr Cys Asn His Arg Asn 
        275                 280                 285             


Lys Arg Gly Arg Lys Lys Leu Leu Tyr Ile Phe Lys Gln Pro Phe Met 
    290                 295                 300                 


Arg Pro Val Gln Thr Thr Gln Glu Glu Asp Gly Cys Ser Cys Arg Phe 
305                 310                 315                 320 


Pro Glu Glu Glu Glu Gly Gly Cys Glu Leu Arg Val Lys Phe Ser Arg 
                325                 330                 335     


Ser Ala Asp Ala Pro Ala Tyr Gln Gln Gly Gln Asn Gln Leu Tyr Asn 
            340                 345                 350         


Glu Leu Asn Leu Gly Arg Arg Glu Glu Tyr Asp Val Leu Asp Lys Arg 
        355                 360                 365             


Arg Gly Arg Asp Pro Glu Met Gly Gly Lys Pro Arg Arg Lys Asn Pro 
    370                 375                 380                 


Gln Glu Gly Leu Tyr Asn Glu Leu Gln Lys Asp Lys Met Ala Glu Ala 
385                 390                 395                 400 


Tyr Ser Glu Ile Gly Met Lys Gly Glu Arg Arg Arg Gly Lys Gly His 
                405                 410                 415     


Asp Gly Leu Tyr Gln Gly Leu Ser Thr Ala Thr Lys Asp Thr Tyr Asp 
            420                 425                 430         


Ala Leu His Met Gln Ala Leu Pro Pro Arg 
        435                 440         


<210>  40
<211>  549
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  scFV GC33/hIgG1 hinge/CD8alpha TMD/F36V-FKBP/41BB/CD3zeta

<400>  40

Gln Val Gln Leu Gln Gln Ser Gly Ala Glu Leu Val Arg Pro Gly Ala 
1               5                   10                  15      


Ser Val Lys Leu Ser Cys Lys Ala Ser Gly Tyr Thr Phe Thr Asp Tyr 
            20                  25                  30          


Glu Met His Trp Val Lys Gln Thr Pro Val His Gly Leu Lys Trp Ile 
        35                  40                  45              


Gly Ala Leu Asp Pro Lys Thr Gly Asp Thr Ala Tyr Ser Gln Lys Phe 
    50                  55                  60                  


Lys Gly Lys Ala Thr Leu Thr Ala Asp Lys Ser Ser Ser Thr Ala Tyr 
65                  70                  75                  80  


Met Glu Leu Arg Ser Leu Thr Ser Glu Asp Ser Ala Val Tyr Tyr Cys 
                85                  90                  95      


Thr Arg Phe Tyr Ser Tyr Thr Tyr Trp Gly Gln Gly Thr Leu Val Thr 
            100                 105                 110         


Val Ser Ala Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly 
        115                 120                 125             


Gly Ser Asp Val Val Met Thr Gln Thr Pro Leu Ser Leu Pro Val Ser 
    130                 135                 140                 


Leu Gly Asp Gln Ala Ser Ile Ser Cys Arg Ser Ser Gln Ser Leu Val 
145                 150                 155                 160 


His Ser Asn Gly Asn Thr Tyr Leu His Trp Tyr Leu Gln Lys Pro Gly 
                165                 170                 175     


Gln Ser Pro Lys Leu Leu Ile Tyr Lys Val Ser Asn Arg Phe Ser Gly 
            180                 185                 190         


Val Pro Asp Arg Phe Ser Gly Ser Gly Ser Gly Thr Asp Phe Thr Leu 
        195                 200                 205             


Lys Ile Ser Arg Val Glu Ala Glu Asp Leu Gly Val Tyr Phe Cys Ser 
    210                 215                 220                 


Gln Asn Thr His Val Pro Pro Thr Phe Gly Ser Gly Thr Lys Leu Glu 
225                 230                 235                 240 


Ile Lys Glu Pro Lys Ser Cys Asp Lys Thr His Thr Cys Pro Pro Cys 
                245                 250                 255     


Pro Asp Pro Lys Ile Tyr Ile Trp Ala Pro Leu Ala Gly Thr Cys Gly 
            260                 265                 270         


Val Leu Leu Leu Ser Leu Val Ile Thr Leu Tyr Cys Asn His Arg Asn 
        275                 280                 285             


Gly Val Gln Val Glu Thr Ile Ser Pro Gly Asp Gly Arg Thr Phe Pro 
    290                 295                 300                 


Lys Arg Gly Gln Thr Cys Val Val His Tyr Thr Gly Met Leu Glu Asp 
305                 310                 315                 320 


Gly Lys Lys Val Asp Ser Ser Arg Asp Arg Asn Lys Pro Phe Lys Phe 
                325                 330                 335     


Met Leu Gly Lys Gln Glu Val Ile Arg Gly Trp Glu Glu Gly Val Ala 
            340                 345                 350         


Gln Met Ser Val Gly Gln Arg Ala Lys Leu Thr Ile Ser Pro Asp Tyr 
        355                 360                 365             


Ala Tyr Gly Ala Thr Gly His Pro Gly Ile Ile Pro Pro His Ala Thr 
    370                 375                 380                 


Leu Val Phe Asp Val Glu Leu Leu Lys Leu Glu Lys Arg Gly Arg Lys 
385                 390                 395                 400 


Lys Leu Leu Tyr Ile Phe Lys Gln Pro Phe Met Arg Pro Val Gln Thr 
                405                 410                 415     


Thr Gln Glu Glu Asp Gly Cys Ser Cys Arg Phe Pro Glu Glu Glu Glu 
            420                 425                 430         


Gly Gly Cys Glu Leu Arg Val Lys Phe Ser Arg Ser Ala Asp Ala Pro 
        435                 440                 445             


Ala Tyr Gln Gln Gly Gln Asn Gln Leu Tyr Asn Glu Leu Asn Leu Gly 
    450                 455                 460                 


Arg Arg Glu Glu Tyr Asp Val Leu Asp Lys Arg Arg Gly Arg Asp Pro 
465                 470                 475                 480 


Glu Met Gly Gly Lys Pro Arg Arg Lys Asn Pro Gln Glu Gly Leu Tyr 
                485                 490                 495     


Asn Glu Leu Gln Lys Asp Lys Met Ala Glu Ala Tyr Ser Glu Ile Gly 
            500                 505                 510         


Met Lys Gly Glu Arg Arg Arg Gly Lys Gly His Asp Gly Leu Tyr Gln 
        515                 520                 525             


Gly Leu Ser Thr Ala Thr Lys Asp Thr Tyr Asp Ala Leu His Met Gln 
    530                 535                 540                 


Ala Leu Pro Pro Arg 
545                 


<210>  41
<211>  444
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  scFV GC33/hIgG1 hinge/CD8alpha TMD/CD28/CD3zeta

<400>  41

Gln Val Gln Leu Gln Gln Ser Gly Ala Glu Leu Val Arg Pro Gly Ala 
1               5                   10                  15      


Ser Val Lys Leu Ser Cys Lys Ala Ser Gly Tyr Thr Phe Thr Asp Tyr 
            20                  25                  30          


Glu Met His Trp Val Lys Gln Thr Pro Val His Gly Leu Lys Trp Ile 
        35                  40                  45              


Gly Ala Leu Asp Pro Lys Thr Gly Asp Thr Ala Tyr Ser Gln Lys Phe 
    50                  55                  60                  


Lys Gly Lys Ala Thr Leu Thr Ala Asp Lys Ser Ser Ser Thr Ala Tyr 
65                  70                  75                  80  


Met Glu Leu Arg Ser Leu Thr Ser Glu Asp Ser Ala Val Tyr Tyr Cys 
                85                  90                  95      


Thr Arg Phe Tyr Ser Tyr Thr Tyr Trp Gly Gln Gly Thr Leu Val Thr 
            100                 105                 110         


Val Ser Ala Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly 
        115                 120                 125             


Gly Ser Asp Val Val Met Thr Gln Thr Pro Leu Ser Leu Pro Val Ser 
    130                 135                 140                 


Leu Gly Asp Gln Ala Ser Ile Ser Cys Arg Ser Ser Gln Ser Leu Val 
145                 150                 155                 160 


His Ser Asn Gly Asn Thr Tyr Leu His Trp Tyr Leu Gln Lys Pro Gly 
                165                 170                 175     


Gln Ser Pro Lys Leu Leu Ile Tyr Lys Val Ser Asn Arg Phe Ser Gly 
            180                 185                 190         


Val Pro Asp Arg Phe Ser Gly Ser Gly Ser Gly Thr Asp Phe Thr Leu 
        195                 200                 205             


Lys Ile Ser Arg Val Glu Ala Glu Asp Leu Gly Val Tyr Phe Cys Ser 
    210                 215                 220                 


Gln Asn Thr His Val Pro Pro Thr Phe Gly Ser Gly Thr Lys Leu Glu 
225                 230                 235                 240 


Ile Lys Glu Pro Lys Ser Cys Asp Lys Thr His Thr Cys Pro Pro Cys 
                245                 250                 255     


Pro Asp Pro Lys Ile Tyr Ile Trp Ala Pro Leu Ala Gly Thr Cys Gly 
            260                 265                 270         


Val Leu Leu Leu Ser Leu Val Ile Thr Leu Tyr Cys Asn His Arg Asn 
        275                 280                 285             


Phe Trp Val Arg Ser Lys Arg Ser Arg Leu Leu His Ser Asp Tyr Met 
    290                 295                 300                 


Asn Met Thr Pro Arg Arg Pro Gly Pro Thr Arg Lys His Tyr Gln Pro 
305                 310                 315                 320 


Tyr Ala Pro Pro Arg Asp Phe Ala Ala Tyr Arg Ser Arg Val Lys Phe 
                325                 330                 335     


Ser Arg Ser Ala Asp Ala Pro Ala Tyr Gln Gln Gly Gln Asn Gln Leu 
            340                 345                 350         


Tyr Asn Glu Leu Asn Leu Gly Arg Arg Glu Glu Tyr Asp Val Leu Asp 
        355                 360                 365             


Lys Arg Arg Gly Arg Asp Pro Glu Met Gly Gly Lys Pro Arg Arg Lys 
    370                 375                 380                 


Asn Pro Gln Glu Gly Leu Tyr Asn Glu Leu Gln Lys Asp Lys Met Ala 
385                 390                 395                 400 


Glu Ala Tyr Ser Glu Ile Gly Met Lys Gly Glu Arg Arg Arg Gly Lys 
                405                 410                 415     


Gly His Asp Gly Leu Tyr Gln Gly Leu Ser Thr Ala Thr Lys Asp Thr 
            420                 425                 430         


Tyr Asp Ala Leu His Met Gln Ala Leu Pro Pro Arg 
        435                 440                 


<210>  42
<211>  551
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  scFV GC33/hIgG1 hinge/CD8alpha TMD/F36V-FKBP/CD28/CD3zeta

<400>  42

Gln Val Gln Leu Gln Gln Ser Gly Ala Glu Leu Val Arg Pro Gly Ala 
1               5                   10                  15      


Ser Val Lys Leu Ser Cys Lys Ala Ser Gly Tyr Thr Phe Thr Asp Tyr 
            20                  25                  30          


Glu Met His Trp Val Lys Gln Thr Pro Val His Gly Leu Lys Trp Ile 
        35                  40                  45              


Gly Ala Leu Asp Pro Lys Thr Gly Asp Thr Ala Tyr Ser Gln Lys Phe 
    50                  55                  60                  


Lys Gly Lys Ala Thr Leu Thr Ala Asp Lys Ser Ser Ser Thr Ala Tyr 
65                  70                  75                  80  


Met Glu Leu Arg Ser Leu Thr Ser Glu Asp Ser Ala Val Tyr Tyr Cys 
                85                  90                  95      


Thr Arg Phe Tyr Ser Tyr Thr Tyr Trp Gly Gln Gly Thr Leu Val Thr 
            100                 105                 110         


Val Ser Ala Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly 
        115                 120                 125             


Gly Ser Asp Val Val Met Thr Gln Thr Pro Leu Ser Leu Pro Val Ser 
    130                 135                 140                 


Leu Gly Asp Gln Ala Ser Ile Ser Cys Arg Ser Ser Gln Ser Leu Val 
145                 150                 155                 160 


His Ser Asn Gly Asn Thr Tyr Leu His Trp Tyr Leu Gln Lys Pro Gly 
                165                 170                 175     


Gln Ser Pro Lys Leu Leu Ile Tyr Lys Val Ser Asn Arg Phe Ser Gly 
            180                 185                 190         


Val Pro Asp Arg Phe Ser Gly Ser Gly Ser Gly Thr Asp Phe Thr Leu 
        195                 200                 205             


Lys Ile Ser Arg Val Glu Ala Glu Asp Leu Gly Val Tyr Phe Cys Ser 
    210                 215                 220                 


Gln Asn Thr His Val Pro Pro Thr Phe Gly Ser Gly Thr Lys Leu Glu 
225                 230                 235                 240 


Ile Lys Glu Pro Lys Ser Cys Asp Lys Thr His Thr Cys Pro Pro Cys 
                245                 250                 255     


Pro Asp Pro Lys Ile Tyr Ile Trp Ala Pro Leu Ala Gly Thr Cys Gly 
            260                 265                 270         


Val Leu Leu Leu Ser Leu Val Ile Thr Leu Tyr Cys Asn His Arg Asn 
        275                 280                 285             


Gly Val Gln Val Glu Thr Ile Ser Pro Gly Asp Gly Arg Thr Phe Pro 
    290                 295                 300                 


Lys Arg Gly Gln Thr Cys Val Val His Tyr Thr Gly Met Leu Glu Asp 
305                 310                 315                 320 


Gly Lys Lys Val Asp Ser Ser Arg Asp Arg Asn Lys Pro Phe Lys Phe 
                325                 330                 335     


Met Leu Gly Lys Gln Glu Val Ile Arg Gly Trp Glu Glu Gly Val Ala 
            340                 345                 350         


Gln Met Ser Val Gly Gln Arg Ala Lys Leu Thr Ile Ser Pro Asp Tyr 
        355                 360                 365             


Ala Tyr Gly Ala Thr Gly His Pro Gly Ile Ile Pro Pro His Ala Thr 
    370                 375                 380                 


Leu Val Phe Asp Val Glu Leu Leu Lys Leu Glu Phe Trp Val Arg Ser 
385                 390                 395                 400 


Lys Arg Ser Arg Leu Leu His Ser Asp Tyr Met Asn Met Thr Pro Arg 
                405                 410                 415     


Arg Pro Gly Pro Thr Arg Lys His Tyr Gln Pro Tyr Ala Pro Pro Arg 
            420                 425                 430         


Asp Phe Ala Ala Tyr Arg Ser Arg Val Lys Phe Ser Arg Ser Ala Asp 
        435                 440                 445             


Ala Pro Ala Tyr Gln Gln Gly Gln Asn Gln Leu Tyr Asn Glu Leu Asn 
    450                 455                 460                 


Leu Gly Arg Arg Glu Glu Tyr Asp Val Leu Asp Lys Arg Arg Gly Arg 
465                 470                 475                 480 


Asp Pro Glu Met Gly Gly Lys Pro Arg Arg Lys Asn Pro Gln Glu Gly 
                485                 490                 495     


Leu Tyr Asn Glu Leu Gln Lys Asp Lys Met Ala Glu Ala Tyr Ser Glu 
            500                 505                 510         


Ile Gly Met Lys Gly Glu Arg Arg Arg Gly Lys Gly His Asp Gly Leu 
        515                 520                 525             


Tyr Gln Gly Leu Ser Thr Ala Thr Lys Asp Thr Tyr Asp Ala Leu His 
    530                 535                 540                 


Met Gln Ala Leu Pro Pro Arg 
545                 550     


<210>  43
<211>  564
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  hIgG heavy chain signal sequence/scFV GC33/hIgG1 hinge/CD28 
       TMD/F36V-FKBP/41BB/CD3zeta

<400>  43

Met Asp Trp Ile Trp Arg Ile Leu Phe Leu Val Gly Ala Ala Thr Gly 
1               5                   10                  15      


Ala His Ser Gln Val Gln Leu Gln Gln Ser Gly Ala Glu Leu Val Arg 
            20                  25                  30          


Pro Gly Ala Ser Val Lys Leu Ser Cys Lys Ala Ser Gly Tyr Thr Phe 
        35                  40                  45              


Thr Asp Tyr Glu Met His Trp Val Lys Gln Thr Pro Val His Gly Leu 
    50                  55                  60                  


Lys Trp Ile Gly Ala Leu Asp Pro Lys Thr Gly Asp Thr Ala Tyr Ser 
65                  70                  75                  80  


Gln Lys Phe Lys Gly Lys Ala Thr Leu Thr Ala Asp Lys Ser Ser Ser 
                85                  90                  95      


Thr Ala Tyr Met Glu Leu Arg Ser Leu Thr Ser Glu Asp Ser Ala Val 
            100                 105                 110         


Tyr Tyr Cys Thr Arg Phe Tyr Ser Tyr Thr Tyr Trp Gly Gln Gly Thr 
        115                 120                 125             


Leu Val Thr Val Ser Ala Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser 
    130                 135                 140                 


Gly Gly Gly Gly Ser Asp Val Val Met Thr Gln Thr Pro Leu Ser Leu 
145                 150                 155                 160 


Pro Val Ser Leu Gly Asp Gln Ala Ser Ile Ser Cys Arg Ser Ser Gln 
                165                 170                 175     


Ser Leu Val His Ser Asn Gly Asn Thr Tyr Leu His Trp Tyr Leu Gln 
            180                 185                 190         


Lys Pro Gly Gln Ser Pro Lys Leu Leu Ile Tyr Lys Val Ser Asn Arg 
        195                 200                 205             


Phe Ser Gly Val Pro Asp Arg Phe Ser Gly Ser Gly Ser Gly Thr Asp 
    210                 215                 220                 


Phe Thr Leu Lys Ile Ser Arg Val Glu Ala Glu Asp Leu Gly Val Tyr 
225                 230                 235                 240 


Phe Cys Ser Gln Asn Thr His Val Pro Pro Thr Phe Gly Ser Gly Thr 
                245                 250                 255     


Lys Leu Glu Ile Lys Glu Pro Lys Ser Cys Asp Lys Thr His Thr Cys 
            260                 265                 270         


Pro Pro Cys Pro Asp Pro Lys Phe Trp Val Leu Val Val Val Gly Gly 
        275                 280                 285             


Val Leu Ala Cys Tyr Ser Leu Leu Val Thr Val Ala Phe Ile Ile Gly 
    290                 295                 300                 


Val Gln Val Glu Thr Ile Ser Pro Gly Asp Gly Arg Thr Phe Pro Lys 
305                 310                 315                 320 


Arg Gly Gln Thr Cys Val Val His Tyr Thr Gly Met Leu Glu Asp Gly 
                325                 330                 335     


Lys Lys Val Asp Ser Ser Arg Asp Arg Asn Lys Pro Phe Lys Phe Met 
            340                 345                 350         


Leu Gly Lys Gln Glu Val Ile Arg Gly Trp Glu Glu Gly Val Ala Gln 
        355                 360                 365             


Met Ser Val Gly Gln Arg Ala Lys Leu Thr Ile Ser Pro Asp Tyr Ala 
    370                 375                 380                 


Tyr Gly Ala Thr Gly His Pro Gly Ile Ile Pro Pro His Ala Thr Leu 
385                 390                 395                 400 


Val Phe Asp Val Glu Leu Leu Lys Leu Glu Lys Arg Gly Arg Lys Lys 
                405                 410                 415     


Leu Leu Tyr Ile Phe Lys Gln Pro Phe Met Arg Pro Val Gln Thr Thr 
            420                 425                 430         


Gln Glu Glu Asp Gly Cys Ser Cys Arg Phe Pro Glu Glu Glu Glu Gly 
        435                 440                 445             


Gly Cys Glu Leu Arg Val Lys Phe Ser Arg Ser Ala Asp Ala Pro Ala 
    450                 455                 460                 


Tyr Gln Gln Gly Gln Asn Gln Leu Tyr Asn Glu Leu Asn Leu Gly Arg 
465                 470                 475                 480 


Arg Glu Glu Tyr Asp Val Leu Asp Lys Arg Arg Gly Arg Asp Pro Glu 
                485                 490                 495     


Met Gly Gly Lys Pro Arg Arg Lys Asn Pro Gln Glu Gly Leu Tyr Asn 
            500                 505                 510         


Glu Leu Gln Lys Asp Lys Met Ala Glu Ala Tyr Ser Glu Ile Gly Met 
        515                 520                 525             


Lys Gly Glu Arg Arg Arg Gly Lys Gly His Asp Gly Leu Tyr Gln Gly 
    530                 535                 540                 


Leu Ser Thr Ala Thr Lys Asp Thr Tyr Asp Ala Leu His Met Gln Ala 
545                 550                 555                 560 


Leu Pro Pro Arg 
                


<210>  44
<211>  461
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  hIgG heavy chain signal sequence/scFV GC33/hIgG1 hinge/CD8alpha 
       TMD/41BB/CD3zeta

<400>  44

Met Asp Trp Ile Trp Arg Ile Leu Phe Leu Val Gly Ala Ala Thr Gly 
1               5                   10                  15      


Ala His Ser Gln Val Gln Leu Gln Gln Ser Gly Ala Glu Leu Val Arg 
            20                  25                  30          


Pro Gly Ala Ser Val Lys Leu Ser Cys Lys Ala Ser Gly Tyr Thr Phe 
        35                  40                  45              


Thr Asp Tyr Glu Met His Trp Val Lys Gln Thr Pro Val His Gly Leu 
    50                  55                  60                  


Lys Trp Ile Gly Ala Leu Asp Pro Lys Thr Gly Asp Thr Ala Tyr Ser 
65                  70                  75                  80  


Gln Lys Phe Lys Gly Lys Ala Thr Leu Thr Ala Asp Lys Ser Ser Ser 
                85                  90                  95      


Thr Ala Tyr Met Glu Leu Arg Ser Leu Thr Ser Glu Asp Ser Ala Val 
            100                 105                 110         


Tyr Tyr Cys Thr Arg Phe Tyr Ser Tyr Thr Tyr Trp Gly Gln Gly Thr 
        115                 120                 125             


Leu Val Thr Val Ser Ala Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser 
    130                 135                 140                 


Gly Gly Gly Gly Ser Asp Val Val Met Thr Gln Thr Pro Leu Ser Leu 
145                 150                 155                 160 


Pro Val Ser Leu Gly Asp Gln Ala Ser Ile Ser Cys Arg Ser Ser Gln 
                165                 170                 175     


Ser Leu Val His Ser Asn Gly Asn Thr Tyr Leu His Trp Tyr Leu Gln 
            180                 185                 190         


Lys Pro Gly Gln Ser Pro Lys Leu Leu Ile Tyr Lys Val Ser Asn Arg 
        195                 200                 205             


Phe Ser Gly Val Pro Asp Arg Phe Ser Gly Ser Gly Ser Gly Thr Asp 
    210                 215                 220                 


Phe Thr Leu Lys Ile Ser Arg Val Glu Ala Glu Asp Leu Gly Val Tyr 
225                 230                 235                 240 


Phe Cys Ser Gln Asn Thr His Val Pro Pro Thr Phe Gly Ser Gly Thr 
                245                 250                 255     


Lys Leu Glu Ile Lys Glu Pro Lys Ser Cys Asp Lys Thr His Thr Cys 
            260                 265                 270         


Pro Pro Cys Pro Asp Pro Lys Ile Tyr Ile Trp Ala Pro Leu Ala Gly 
        275                 280                 285             


Thr Cys Gly Val Leu Leu Leu Ser Leu Val Ile Thr Leu Tyr Cys Asn 
    290                 295                 300                 


His Arg Asn Lys Arg Gly Arg Lys Lys Leu Leu Tyr Ile Phe Lys Gln 
305                 310                 315                 320 


Pro Phe Met Arg Pro Val Gln Thr Thr Gln Glu Glu Asp Gly Cys Ser 
                325                 330                 335     


Cys Arg Phe Pro Glu Glu Glu Glu Gly Gly Cys Glu Leu Arg Val Lys 
            340                 345                 350         


Phe Ser Arg Ser Ala Asp Ala Pro Ala Tyr Gln Gln Gly Gln Asn Gln 
        355                 360                 365             


Leu Tyr Asn Glu Leu Asn Leu Gly Arg Arg Glu Glu Tyr Asp Val Leu 
    370                 375                 380                 


Asp Lys Arg Arg Gly Arg Asp Pro Glu Met Gly Gly Lys Pro Arg Arg 
385                 390                 395                 400 


Lys Asn Pro Gln Glu Gly Leu Tyr Asn Glu Leu Gln Lys Asp Lys Met 
                405                 410                 415     


Ala Glu Ala Tyr Ser Glu Ile Gly Met Lys Gly Glu Arg Arg Arg Gly 
            420                 425                 430         


Lys Gly His Asp Gly Leu Tyr Gln Gly Leu Ser Thr Ala Thr Lys Asp 
        435                 440                 445             


Thr Tyr Asp Ala Leu His Met Gln Ala Leu Pro Pro Arg 
    450                 455                 460     


<210>  45
<211>  568
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  hIgG heavy chain signal sequence/scFV GC33/hIgG1 hinge/CD8alpha 
       TMD/F36V-FKBP/41BB/CD3zeta

<400>  45

Met Asp Trp Ile Trp Arg Ile Leu Phe Leu Val Gly Ala Ala Thr Gly 
1               5                   10                  15      


Ala His Ser Gln Val Gln Leu Gln Gln Ser Gly Ala Glu Leu Val Arg 
            20                  25                  30          


Pro Gly Ala Ser Val Lys Leu Ser Cys Lys Ala Ser Gly Tyr Thr Phe 
        35                  40                  45              


Thr Asp Tyr Glu Met His Trp Val Lys Gln Thr Pro Val His Gly Leu 
    50                  55                  60                  


Lys Trp Ile Gly Ala Leu Asp Pro Lys Thr Gly Asp Thr Ala Tyr Ser 
65                  70                  75                  80  


Gln Lys Phe Lys Gly Lys Ala Thr Leu Thr Ala Asp Lys Ser Ser Ser 
                85                  90                  95      


Thr Ala Tyr Met Glu Leu Arg Ser Leu Thr Ser Glu Asp Ser Ala Val 
            100                 105                 110         


Tyr Tyr Cys Thr Arg Phe Tyr Ser Tyr Thr Tyr Trp Gly Gln Gly Thr 
        115                 120                 125             


Leu Val Thr Val Ser Ala Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser 
    130                 135                 140                 


Gly Gly Gly Gly Ser Asp Val Val Met Thr Gln Thr Pro Leu Ser Leu 
145                 150                 155                 160 


Pro Val Ser Leu Gly Asp Gln Ala Ser Ile Ser Cys Arg Ser Ser Gln 
                165                 170                 175     


Ser Leu Val His Ser Asn Gly Asn Thr Tyr Leu His Trp Tyr Leu Gln 
            180                 185                 190         


Lys Pro Gly Gln Ser Pro Lys Leu Leu Ile Tyr Lys Val Ser Asn Arg 
        195                 200                 205             


Phe Ser Gly Val Pro Asp Arg Phe Ser Gly Ser Gly Ser Gly Thr Asp 
    210                 215                 220                 


Phe Thr Leu Lys Ile Ser Arg Val Glu Ala Glu Asp Leu Gly Val Tyr 
225                 230                 235                 240 


Phe Cys Ser Gln Asn Thr His Val Pro Pro Thr Phe Gly Ser Gly Thr 
                245                 250                 255     


Lys Leu Glu Ile Lys Glu Pro Lys Ser Cys Asp Lys Thr His Thr Cys 
            260                 265                 270         


Pro Pro Cys Pro Asp Pro Lys Ile Tyr Ile Trp Ala Pro Leu Ala Gly 
        275                 280                 285             


Thr Cys Gly Val Leu Leu Leu Ser Leu Val Ile Thr Leu Tyr Cys Asn 
    290                 295                 300                 


His Arg Asn Gly Val Gln Val Glu Thr Ile Ser Pro Gly Asp Gly Arg 
305                 310                 315                 320 


Thr Phe Pro Lys Arg Gly Gln Thr Cys Val Val His Tyr Thr Gly Met 
                325                 330                 335     


Leu Glu Asp Gly Lys Lys Val Asp Ser Ser Arg Asp Arg Asn Lys Pro 
            340                 345                 350         


Phe Lys Phe Met Leu Gly Lys Gln Glu Val Ile Arg Gly Trp Glu Glu 
        355                 360                 365             


Gly Val Ala Gln Met Ser Val Gly Gln Arg Ala Lys Leu Thr Ile Ser 
    370                 375                 380                 


Pro Asp Tyr Ala Tyr Gly Ala Thr Gly His Pro Gly Ile Ile Pro Pro 
385                 390                 395                 400 


His Ala Thr Leu Val Phe Asp Val Glu Leu Leu Lys Leu Glu Lys Arg 
                405                 410                 415     


Gly Arg Lys Lys Leu Leu Tyr Ile Phe Lys Gln Pro Phe Met Arg Pro 
            420                 425                 430         


Val Gln Thr Thr Gln Glu Glu Asp Gly Cys Ser Cys Arg Phe Pro Glu 
        435                 440                 445             


Glu Glu Glu Gly Gly Cys Glu Leu Arg Val Lys Phe Ser Arg Ser Ala 
    450                 455                 460                 


Asp Ala Pro Ala Tyr Gln Gln Gly Gln Asn Gln Leu Tyr Asn Glu Leu 
465                 470                 475                 480 


Asn Leu Gly Arg Arg Glu Glu Tyr Asp Val Leu Asp Lys Arg Arg Gly 
                485                 490                 495     


Arg Asp Pro Glu Met Gly Gly Lys Pro Arg Arg Lys Asn Pro Gln Glu 
            500                 505                 510         


Gly Leu Tyr Asn Glu Leu Gln Lys Asp Lys Met Ala Glu Ala Tyr Ser 
        515                 520                 525             


Glu Ile Gly Met Lys Gly Glu Arg Arg Arg Gly Lys Gly His Asp Gly 
    530                 535                 540                 


Leu Tyr Gln Gly Leu Ser Thr Ala Thr Lys Asp Thr Tyr Asp Ala Leu 
545                 550                 555                 560 


His Met Gln Ala Leu Pro Pro Arg 
                565             


<210>  46
<211>  463
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  hIgG heavy chain signal sequence/scFV GC33/hIgG1 hinge/CD8alpha 
       TMD/CD28/CD3zeta

<400>  46

Met Asp Trp Ile Trp Arg Ile Leu Phe Leu Val Gly Ala Ala Thr Gly 
1               5                   10                  15      


Ala His Ser Gln Val Gln Leu Gln Gln Ser Gly Ala Glu Leu Val Arg 
            20                  25                  30          


Pro Gly Ala Ser Val Lys Leu Ser Cys Lys Ala Ser Gly Tyr Thr Phe 
        35                  40                  45              


Thr Asp Tyr Glu Met His Trp Val Lys Gln Thr Pro Val His Gly Leu 
    50                  55                  60                  


Lys Trp Ile Gly Ala Leu Asp Pro Lys Thr Gly Asp Thr Ala Tyr Ser 
65                  70                  75                  80  


Gln Lys Phe Lys Gly Lys Ala Thr Leu Thr Ala Asp Lys Ser Ser Ser 
                85                  90                  95      


Thr Ala Tyr Met Glu Leu Arg Ser Leu Thr Ser Glu Asp Ser Ala Val 
            100                 105                 110         


Tyr Tyr Cys Thr Arg Phe Tyr Ser Tyr Thr Tyr Trp Gly Gln Gly Thr 
        115                 120                 125             


Leu Val Thr Val Ser Ala Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser 
    130                 135                 140                 


Gly Gly Gly Gly Ser Asp Val Val Met Thr Gln Thr Pro Leu Ser Leu 
145                 150                 155                 160 


Pro Val Ser Leu Gly Asp Gln Ala Ser Ile Ser Cys Arg Ser Ser Gln 
                165                 170                 175     


Ser Leu Val His Ser Asn Gly Asn Thr Tyr Leu His Trp Tyr Leu Gln 
            180                 185                 190         


Lys Pro Gly Gln Ser Pro Lys Leu Leu Ile Tyr Lys Val Ser Asn Arg 
        195                 200                 205             


Phe Ser Gly Val Pro Asp Arg Phe Ser Gly Ser Gly Ser Gly Thr Asp 
    210                 215                 220                 


Phe Thr Leu Lys Ile Ser Arg Val Glu Ala Glu Asp Leu Gly Val Tyr 
225                 230                 235                 240 


Phe Cys Ser Gln Asn Thr His Val Pro Pro Thr Phe Gly Ser Gly Thr 
                245                 250                 255     


Lys Leu Glu Ile Lys Glu Pro Lys Ser Cys Asp Lys Thr His Thr Cys 
            260                 265                 270         


Pro Pro Cys Pro Asp Pro Lys Ile Tyr Ile Trp Ala Pro Leu Ala Gly 
        275                 280                 285             


Thr Cys Gly Val Leu Leu Leu Ser Leu Val Ile Thr Leu Tyr Cys Asn 
    290                 295                 300                 


His Arg Asn Phe Trp Val Arg Ser Lys Arg Ser Arg Leu Leu His Ser 
305                 310                 315                 320 


Asp Tyr Met Asn Met Thr Pro Arg Arg Pro Gly Pro Thr Arg Lys His 
                325                 330                 335     


Tyr Gln Pro Tyr Ala Pro Pro Arg Asp Phe Ala Ala Tyr Arg Ser Arg 
            340                 345                 350         


Val Lys Phe Ser Arg Ser Ala Asp Ala Pro Ala Tyr Gln Gln Gly Gln 
        355                 360                 365             


Asn Gln Leu Tyr Asn Glu Leu Asn Leu Gly Arg Arg Glu Glu Tyr Asp 
    370                 375                 380                 


Val Leu Asp Lys Arg Arg Gly Arg Asp Pro Glu Met Gly Gly Lys Pro 
385                 390                 395                 400 


Arg Arg Lys Asn Pro Gln Glu Gly Leu Tyr Asn Glu Leu Gln Lys Asp 
                405                 410                 415     


Lys Met Ala Glu Ala Tyr Ser Glu Ile Gly Met Lys Gly Glu Arg Arg 
            420                 425                 430         


Arg Gly Lys Gly His Asp Gly Leu Tyr Gln Gly Leu Ser Thr Ala Thr 
        435                 440                 445             


Lys Asp Thr Tyr Asp Ala Leu His Met Gln Ala Leu Pro Pro Arg 
    450                 455                 460             


<210>  47
<211>  570
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  hIgG heavy chain signal sequence/scFV GC33/hIgG1 hinge/CD8alpha 
       TMD/F36V-FKBP/CD28/CD3zeta

<400>  47

Met Asp Trp Ile Trp Arg Ile Leu Phe Leu Val Gly Ala Ala Thr Gly 
1               5                   10                  15      


Ala His Ser Gln Val Gln Leu Gln Gln Ser Gly Ala Glu Leu Val Arg 
            20                  25                  30          


Pro Gly Ala Ser Val Lys Leu Ser Cys Lys Ala Ser Gly Tyr Thr Phe 
        35                  40                  45              


Thr Asp Tyr Glu Met His Trp Val Lys Gln Thr Pro Val His Gly Leu 
    50                  55                  60                  


Lys Trp Ile Gly Ala Leu Asp Pro Lys Thr Gly Asp Thr Ala Tyr Ser 
65                  70                  75                  80  


Gln Lys Phe Lys Gly Lys Ala Thr Leu Thr Ala Asp Lys Ser Ser Ser 
                85                  90                  95      


Thr Ala Tyr Met Glu Leu Arg Ser Leu Thr Ser Glu Asp Ser Ala Val 
            100                 105                 110         


Tyr Tyr Cys Thr Arg Phe Tyr Ser Tyr Thr Tyr Trp Gly Gln Gly Thr 
        115                 120                 125             


Leu Val Thr Val Ser Ala Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser 
    130                 135                 140                 


Gly Gly Gly Gly Ser Asp Val Val Met Thr Gln Thr Pro Leu Ser Leu 
145                 150                 155                 160 


Pro Val Ser Leu Gly Asp Gln Ala Ser Ile Ser Cys Arg Ser Ser Gln 
                165                 170                 175     


Ser Leu Val His Ser Asn Gly Asn Thr Tyr Leu His Trp Tyr Leu Gln 
            180                 185                 190         


Lys Pro Gly Gln Ser Pro Lys Leu Leu Ile Tyr Lys Val Ser Asn Arg 
        195                 200                 205             


Phe Ser Gly Val Pro Asp Arg Phe Ser Gly Ser Gly Ser Gly Thr Asp 
    210                 215                 220                 


Phe Thr Leu Lys Ile Ser Arg Val Glu Ala Glu Asp Leu Gly Val Tyr 
225                 230                 235                 240 


Phe Cys Ser Gln Asn Thr His Val Pro Pro Thr Phe Gly Ser Gly Thr 
                245                 250                 255     


Lys Leu Glu Ile Lys Glu Pro Lys Ser Cys Asp Lys Thr His Thr Cys 
            260                 265                 270         


Pro Pro Cys Pro Asp Pro Lys Ile Tyr Ile Trp Ala Pro Leu Ala Gly 
        275                 280                 285             


Thr Cys Gly Val Leu Leu Leu Ser Leu Val Ile Thr Leu Tyr Cys Asn 
    290                 295                 300                 


His Arg Asn Gly Val Gln Val Glu Thr Ile Ser Pro Gly Asp Gly Arg 
305                 310                 315                 320 


Thr Phe Pro Lys Arg Gly Gln Thr Cys Val Val His Tyr Thr Gly Met 
                325                 330                 335     


Leu Glu Asp Gly Lys Lys Val Asp Ser Ser Arg Asp Arg Asn Lys Pro 
            340                 345                 350         


Phe Lys Phe Met Leu Gly Lys Gln Glu Val Ile Arg Gly Trp Glu Glu 
        355                 360                 365             


Gly Val Ala Gln Met Ser Val Gly Gln Arg Ala Lys Leu Thr Ile Ser 
    370                 375                 380                 


Pro Asp Tyr Ala Tyr Gly Ala Thr Gly His Pro Gly Ile Ile Pro Pro 
385                 390                 395                 400 


His Ala Thr Leu Val Phe Asp Val Glu Leu Leu Lys Leu Glu Phe Trp 
                405                 410                 415     


Val Arg Ser Lys Arg Ser Arg Leu Leu His Ser Asp Tyr Met Asn Met 
            420                 425                 430         


Thr Pro Arg Arg Pro Gly Pro Thr Arg Lys His Tyr Gln Pro Tyr Ala 
        435                 440                 445             


Pro Pro Arg Asp Phe Ala Ala Tyr Arg Ser Arg Val Lys Phe Ser Arg 
    450                 455                 460                 


Ser Ala Asp Ala Pro Ala Tyr Gln Gln Gly Gln Asn Gln Leu Tyr Asn 
465                 470                 475                 480 


Glu Leu Asn Leu Gly Arg Arg Glu Glu Tyr Asp Val Leu Asp Lys Arg 
                485                 490                 495     


Arg Gly Arg Asp Pro Glu Met Gly Gly Lys Pro Arg Arg Lys Asn Pro 
            500                 505                 510         


Gln Glu Gly Leu Tyr Asn Glu Leu Gln Lys Asp Lys Met Ala Glu Ala 
        515                 520                 525             


Tyr Ser Glu Ile Gly Met Lys Gly Glu Arg Arg Arg Gly Lys Gly His 
    530                 535                 540                 


Asp Gly Leu Tyr Gln Gly Leu Ser Thr Ala Thr Lys Asp Thr Tyr Asp 
545                 550                 555                 560 


Ala Leu His Met Gln Ala Leu Pro Pro Arg 
                565                 570 


<210>  48
<211>  113
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  3-17I heavy chain variable region sequence

<400>  48

Gln Val Gln Leu Val Gln Ser Gly Ala Glu Val Lys Lys Pro Gly Ser 
1               5                   10                  15      


Ser Val Lys Val Ser Cys Lys Ala Ser Gly Gly Thr Phe Ser Ser Tyr 
            20                  25                  30          


Ala Ile Ser Trp Val Arg Gln Ala Pro Gly Gln Gly Leu Glu Trp Met 
        35                  40                  45              


Gly Gly Ile Ile Pro Ile Phe Gly Thr Ala Asn Tyr Ala Gln Lys Phe 
    50                  55                  60                  


Gln Gly Arg Val Thr Ile Thr Ala Asp Glu Ser Thr Ser Thr Ala Tyr 
65                  70                  75                  80  


Met Glu Leu Ser Ser Leu Arg Ser Glu Asp Thr Ala Val Tyr Tyr Cys 
                85                  90                  95      


Ala Arg Gly Leu Leu Trp Asn Tyr Trp Gly Gln Gly Thr Leu Val Thr 
            100                 105                 110         


Val 
    


<210>  49
<211>  5
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  3-17I HC-CDR1

<400>  49

Ser Tyr Ala Ile Ser 
1               5   


<210>  50
<211>  17
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  3-17I HC-CDR2

<400>  50

Gly Ile Ile Pro Ile Phe Gly Thr Ala Asn Tyr Ala Gln Lys Phe Gln 
1               5                   10                  15      


Gly 
    


<210>  51
<211>  6
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  3-17I HC-CDR3

<400>  51

Gly Leu Leu Trp Asn Tyr 
1               5       


<210>  52
<211>  109
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  3-17I light chain variable region sequence

<400>  52

Glu Ile Val Met Thr Gln Ser Pro Ala Thr Leu Ser Val Ser Pro Gly 
1               5                   10                  15      


Glu Arg Ala Thr Leu Ser Cys Arg Ala Ser Gln Ser Val Ser Ser Asn 
            20                  25                  30          


Leu Ala Trp Tyr Gln Gln Lys Pro Gly Gln Ala Pro Arg Leu Ile Ile 
        35                  40                  45              


Tyr Gly Ala Ser Thr Thr Ala Ser Gly Ile Pro Ala Arg Phe Ser Ala 
    50                  55                  60                  


Ser Gly Ser Gly Thr Asp Phe Thr Leu Thr Ile Ser Ser Leu Gln Ser 
65                  70                  75                  80  


Glu Asp Phe Ala Val Tyr Tyr Cys Gln Gln Tyr Asn Asn Trp Pro Pro 
                85                  90                  95      


Ala Tyr Thr Phe Gly Gln Gly Thr Lys Leu Glu Ile Lys 
            100                 105                 


<210>  53
<211>  11
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  3-17I LC-CDR1

<400>  53

Arg Ala Ser Gln Ser Val Ser Ser Asn Leu Ala 
1               5                   10      


<210>  54
<211>  7
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  3-17I LC-CDR2

<400>  54

Gly Ala Ser Thr Thr Ala Ser 
1               5           


<210>  55
<211>  11
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  3-17I LC-CDR3

<400>  55

Gln Gln Tyr Asn Asn Trp Pro Pro Ala Tyr Thr 
1               5                   10      


<210>  56
<211>  245
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  3-17I scFv

<400>  56

Gln Val Gln Leu Val Gln Ser Gly Ala Glu Val Lys Lys Pro Gly Ser 
1               5                   10                  15      


Ser Val Lys Val Ser Cys Lys Ala Ser Gly Gly Thr Phe Ser Ser Tyr 
            20                  25                  30          


Ala Ile Ser Trp Val Arg Gln Ala Pro Gly Gln Gly Leu Glu Trp Met 
        35                  40                  45              


Gly Gly Ile Ile Pro Ile Phe Gly Thr Ala Asn Tyr Ala Gln Lys Phe 
    50                  55                  60                  


Gln Gly Arg Val Thr Ile Thr Ala Asp Glu Ser Thr Ser Thr Ala Tyr 
65                  70                  75                  80  


Met Glu Leu Ser Ser Leu Arg Ser Glu Asp Thr Ala Val Tyr Tyr Cys 
                85                  90                  95      


Ala Arg Gly Leu Leu Trp Asn Tyr Trp Gly Gln Gly Thr Leu Val Thr 
            100                 105                 110         


Val Ser Ser Lys Leu Ser Gly Ser Ala Ser Ala Pro Lys Leu Glu Glu 
        115                 120                 125             


Gly Glu Phe Ser Glu Ala Arg Val Glu Ile Val Met Thr Gln Ser Pro 
    130                 135                 140                 


Ala Thr Leu Ser Val Ser Pro Gly Glu Arg Ala Thr Leu Ser Cys Arg 
145                 150                 155                 160 


Ala Ser Gln Ser Val Ser Ser Asn Leu Ala Trp Tyr Gln Gln Lys Pro 
                165                 170                 175     


Gly Gln Ala Pro Arg Leu Ile Ile Tyr Gly Ala Ser Thr Thr Ala Ser 
            180                 185                 190         


Gly Ile Pro Ala Arg Phe Ser Ala Ser Gly Ser Gly Thr Asp Phe Thr 
        195                 200                 205             


Leu Thr Ile Ser Ser Leu Gln Ser Glu Asp Phe Ala Val Tyr Tyr Cys 
    210                 215                 220                 


Gln Gln Tyr Asn Asn Trp Pro Pro Ala Tyr Thr Phe Gly Gln Gly Thr 
225                 230                 235                 240 


Lys Leu Glu Ile Lys 
                245 


<210>  57
<211>  21
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  CD226 TMD

<400>  57

Gly Gly Thr Val Leu Leu Leu Leu Phe Val Ile Ser Ile Thr Thr Ile 
1               5                   10                  15      


Ile Val Ile Phe Leu 
            20      


<210>  58
<211>  61
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  CD226 ICD v1

<400>  58

Asn Arg Arg Arg Arg Arg Glu Arg Arg Asp Leu Phe Thr Glu Ser Trp 
1               5                   10                  15      


Asp Thr Gln Lys Ala Pro Asn Asn Tyr Arg Ser Pro Ile Ser Thr Ser 
            20                  25                  30          


Gln Pro Thr Asn Gln Ser Met Asp Asp Thr Arg Glu Asp Ile Tyr Val 
        35                  40                  45              


Asn Tyr Pro Thr Phe Ser Arg Arg Pro Lys Thr Arg Val 
    50                  55                  60      


<210>  59
<211>  63
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  CD226 ICD v2

<400>  59

Phe Leu Asn Arg Arg Arg Arg Arg Glu Arg Arg Asp Leu Phe Thr Glu 
1               5                   10                  15      


Ser Trp Asp Thr Gln Lys Ala Pro Asn Asn Tyr Arg Ser Pro Ile Ser 
            20                  25                  30          


Thr Ser Gln Pro Thr Asn Gln Ser Met Asp Asp Thr Arg Glu Asp Ile 
        35                  40                  45              


Tyr Val Asn Tyr Pro Thr Phe Ser Arg Arg Pro Lys Thr Arg Val 
    50                  55                  60              


<210>  60
<211>  21
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  CD226 TMD

<400>  60

Gly Gly Thr Val Leu Leu Leu Leu Phe Val Ile Ser Ile Thr Thr Ile 
1               5                   10                  15      


Ile Val Ile Phe Leu 
            20      


<210>  61
<211>  415
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  hIgG heavy chain signal sequence/scFV GC33/hIgG1 hinge/CD28 
       TMD/CD3zeta

<400>  61

Met Asp Trp Ile Trp Arg Ile Leu Phe Leu Val Gly Ala Ala Thr Gly 
1               5                   10                  15      


Ala His Ser Gln Val Gln Leu Gln Gln Ser Gly Ala Glu Leu Val Arg 
            20                  25                  30          


Pro Gly Ala Ser Val Lys Leu Ser Cys Lys Ala Ser Gly Tyr Thr Phe 
        35                  40                  45              


Thr Asp Tyr Glu Met His Trp Val Lys Gln Thr Pro Val His Gly Leu 
    50                  55                  60                  


Lys Trp Ile Gly Ala Leu Asp Pro Lys Thr Gly Asp Thr Ala Tyr Ser 
65                  70                  75                  80  


Gln Lys Phe Lys Gly Lys Ala Thr Leu Thr Ala Asp Lys Ser Ser Ser 
                85                  90                  95      


Thr Ala Tyr Met Glu Leu Arg Ser Leu Thr Ser Glu Asp Ser Ala Val 
            100                 105                 110         


Tyr Tyr Cys Thr Arg Phe Tyr Ser Tyr Thr Tyr Trp Gly Gln Gly Thr 
        115                 120                 125             


Leu Val Thr Val Ser Ala Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser 
    130                 135                 140                 


Gly Gly Gly Gly Ser Asp Val Val Met Thr Gln Thr Pro Leu Ser Leu 
145                 150                 155                 160 


Pro Val Ser Leu Gly Asp Gln Ala Ser Ile Ser Cys Arg Ser Ser Gln 
                165                 170                 175     


Ser Leu Val His Ser Asn Gly Asn Thr Tyr Leu His Trp Tyr Leu Gln 
            180                 185                 190         


Lys Pro Gly Gln Ser Pro Lys Leu Leu Ile Tyr Lys Val Ser Asn Arg 
        195                 200                 205             


Phe Ser Gly Val Pro Asp Arg Phe Ser Gly Ser Gly Ser Gly Thr Asp 
    210                 215                 220                 


Phe Thr Leu Lys Ile Ser Arg Val Glu Ala Glu Asp Leu Gly Val Tyr 
225                 230                 235                 240 


Phe Cys Ser Gln Asn Thr His Val Pro Pro Thr Phe Gly Ser Gly Thr 
                245                 250                 255     


Lys Leu Glu Ile Lys Glu Pro Lys Ser Cys Asp Lys Thr His Thr Cys 
            260                 265                 270         


Pro Pro Cys Pro Asp Pro Lys Phe Trp Val Leu Val Val Val Gly Gly 
        275                 280                 285             


Val Leu Ala Cys Tyr Ser Leu Leu Val Thr Val Ala Phe Ile Ile Arg 
    290                 295                 300                 


Val Lys Phe Ser Arg Ser Ala Asp Ala Pro Ala Tyr Gln Gln Gly Gln 
305                 310                 315                 320 


Asn Gln Leu Tyr Asn Glu Leu Asn Leu Gly Arg Arg Glu Glu Tyr Asp 
                325                 330                 335     


Val Leu Asp Lys Arg Arg Gly Arg Asp Pro Glu Met Gly Gly Lys Pro 
            340                 345                 350         


Arg Arg Lys Asn Pro Gln Glu Gly Leu Tyr Asn Glu Leu Gln Lys Asp 
        355                 360                 365             


Lys Met Ala Glu Ala Tyr Ser Glu Ile Gly Met Lys Gly Glu Arg Arg 
    370                 375                 380                 


Arg Gly Lys Gly His Asp Gly Leu Tyr Gln Gly Leu Ser Thr Ala Thr 
385                 390                 395                 400 


Lys Asp Thr Tyr Asp Ala Leu His Met Gln Ala Leu Pro Pro Arg 
                405                 410                 415 


<210>  62
<211>  366
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  hIgG heavy chain signal sequence/scFV GC33/hIgG1 hinge/CD28 
       TMD/CD226 ICD v2

<400>  62

Met Asp Trp Ile Trp Arg Ile Leu Phe Leu Val Gly Ala Ala Thr Gly 
1               5                   10                  15      


Ala His Ser Gln Val Gln Leu Gln Gln Ser Gly Ala Glu Leu Val Arg 
            20                  25                  30          


Pro Gly Ala Ser Val Lys Leu Ser Cys Lys Ala Ser Gly Tyr Thr Phe 
        35                  40                  45              


Thr Asp Tyr Glu Met His Trp Val Lys Gln Thr Pro Val His Gly Leu 
    50                  55                  60                  


Lys Trp Ile Gly Ala Leu Asp Pro Lys Thr Gly Asp Thr Ala Tyr Ser 
65                  70                  75                  80  


Gln Lys Phe Lys Gly Lys Ala Thr Leu Thr Ala Asp Lys Ser Ser Ser 
                85                  90                  95      


Thr Ala Tyr Met Glu Leu Arg Ser Leu Thr Ser Glu Asp Ser Ala Val 
            100                 105                 110         


Tyr Tyr Cys Thr Arg Phe Tyr Ser Tyr Thr Tyr Trp Gly Gln Gly Thr 
        115                 120                 125             


Leu Val Thr Val Ser Ala Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser 
    130                 135                 140                 


Gly Gly Gly Gly Ser Asp Val Val Met Thr Gln Thr Pro Leu Ser Leu 
145                 150                 155                 160 


Pro Val Ser Leu Gly Asp Gln Ala Ser Ile Ser Cys Arg Ser Ser Gln 
                165                 170                 175     


Ser Leu Val His Ser Asn Gly Asn Thr Tyr Leu His Trp Tyr Leu Gln 
            180                 185                 190         


Lys Pro Gly Gln Ser Pro Lys Leu Leu Ile Tyr Lys Val Ser Asn Arg 
        195                 200                 205             


Phe Ser Gly Val Pro Asp Arg Phe Ser Gly Ser Gly Ser Gly Thr Asp 
    210                 215                 220                 


Phe Thr Leu Lys Ile Ser Arg Val Glu Ala Glu Asp Leu Gly Val Tyr 
225                 230                 235                 240 


Phe Cys Ser Gln Asn Thr His Val Pro Pro Thr Phe Gly Ser Gly Thr 
                245                 250                 255     


Lys Leu Glu Ile Lys Glu Pro Lys Ser Cys Asp Lys Thr His Thr Cys 
            260                 265                 270         


Pro Pro Cys Pro Asp Pro Lys Phe Trp Val Leu Val Val Val Gly Gly 
        275                 280                 285             


Val Leu Ala Cys Tyr Ser Leu Leu Val Thr Val Ala Phe Ile Ile Phe 
    290                 295                 300                 


Leu Asn Arg Arg Arg Arg Arg Glu Arg Arg Asp Leu Phe Thr Glu Ser 
305                 310                 315                 320 


Trp Asp Thr Gln Lys Ala Pro Asn Asn Tyr Arg Ser Pro Ile Ser Thr 
                325                 330                 335     


Ser Gln Pro Thr Asn Gln Ser Met Asp Asp Thr Arg Glu Asp Ile Tyr 
            340                 345                 350         


Val Asn Tyr Pro Thr Phe Ser Arg Arg Pro Lys Thr Arg Val 
        355                 360                 365     


<210>  63
<211>  457
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  hIgG heavy chain signal sequence/scFV GC33/hIgG1 hinge/CD28 
       TMD/41BB/CD3zeta

<400>  63

Met Asp Trp Ile Trp Arg Ile Leu Phe Leu Val Gly Ala Ala Thr Gly 
1               5                   10                  15      


Ala His Ser Gln Val Gln Leu Gln Gln Ser Gly Ala Glu Leu Val Arg 
            20                  25                  30          


Pro Gly Ala Ser Val Lys Leu Ser Cys Lys Ala Ser Gly Tyr Thr Phe 
        35                  40                  45              


Thr Asp Tyr Glu Met His Trp Val Lys Gln Thr Pro Val His Gly Leu 
    50                  55                  60                  


Lys Trp Ile Gly Ala Leu Asp Pro Lys Thr Gly Asp Thr Ala Tyr Ser 
65                  70                  75                  80  


Gln Lys Phe Lys Gly Lys Ala Thr Leu Thr Ala Asp Lys Ser Ser Ser 
                85                  90                  95      


Thr Ala Tyr Met Glu Leu Arg Ser Leu Thr Ser Glu Asp Ser Ala Val 
            100                 105                 110         


Tyr Tyr Cys Thr Arg Phe Tyr Ser Tyr Thr Tyr Trp Gly Gln Gly Thr 
        115                 120                 125             


Leu Val Thr Val Ser Ala Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser 
    130                 135                 140                 


Gly Gly Gly Gly Ser Asp Val Val Met Thr Gln Thr Pro Leu Ser Leu 
145                 150                 155                 160 


Pro Val Ser Leu Gly Asp Gln Ala Ser Ile Ser Cys Arg Ser Ser Gln 
                165                 170                 175     


Ser Leu Val His Ser Asn Gly Asn Thr Tyr Leu His Trp Tyr Leu Gln 
            180                 185                 190         


Lys Pro Gly Gln Ser Pro Lys Leu Leu Ile Tyr Lys Val Ser Asn Arg 
        195                 200                 205             


Phe Ser Gly Val Pro Asp Arg Phe Ser Gly Ser Gly Ser Gly Thr Asp 
    210                 215                 220                 


Phe Thr Leu Lys Ile Ser Arg Val Glu Ala Glu Asp Leu Gly Val Tyr 
225                 230                 235                 240 


Phe Cys Ser Gln Asn Thr His Val Pro Pro Thr Phe Gly Ser Gly Thr 
                245                 250                 255     


Lys Leu Glu Ile Lys Glu Pro Lys Ser Cys Asp Lys Thr His Thr Cys 
            260                 265                 270         


Pro Pro Cys Pro Asp Pro Lys Phe Trp Val Leu Val Val Val Gly Gly 
        275                 280                 285             


Val Leu Ala Cys Tyr Ser Leu Leu Val Thr Val Ala Phe Ile Ile Lys 
    290                 295                 300                 


Arg Gly Arg Lys Lys Leu Leu Tyr Ile Phe Lys Gln Pro Phe Met Arg 
305                 310                 315                 320 


Pro Val Gln Thr Thr Gln Glu Glu Asp Gly Cys Ser Cys Arg Phe Pro 
                325                 330                 335     


Glu Glu Glu Glu Gly Gly Cys Glu Leu Arg Val Lys Phe Ser Arg Ser 
            340                 345                 350         


Ala Asp Ala Pro Ala Tyr Gln Gln Gly Gln Asn Gln Leu Tyr Asn Glu 
        355                 360                 365             


Leu Asn Leu Gly Arg Arg Glu Glu Tyr Asp Val Leu Asp Lys Arg Arg 
    370                 375                 380                 


Gly Arg Asp Pro Glu Met Gly Gly Lys Pro Arg Arg Lys Asn Pro Gln 
385                 390                 395                 400 


Glu Gly Leu Tyr Asn Glu Leu Gln Lys Asp Lys Met Ala Glu Ala Tyr 
                405                 410                 415     


Ser Glu Ile Gly Met Lys Gly Glu Arg Arg Arg Gly Lys Gly His Asp 
            420                 425                 430         


Gly Leu Tyr Gln Gly Leu Ser Thr Ala Thr Lys Asp Thr Tyr Asp Ala 
        435                 440                 445             


Leu His Met Gln Ala Leu Pro Pro Arg 
    450                 455         


<210>  64
<211>  518
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  hIgG heavy chain signal sequence/scFV GC33/hIgG1 hinge/CD28 TMD/ 
       CD226 ICD v1/41BB/CD3zeta

<400>  64

Met Asp Trp Ile Trp Arg Ile Leu Phe Leu Val Gly Ala Ala Thr Gly 
1               5                   10                  15      


Ala His Ser Gln Val Gln Leu Gln Gln Ser Gly Ala Glu Leu Val Arg 
            20                  25                  30          


Pro Gly Ala Ser Val Lys Leu Ser Cys Lys Ala Ser Gly Tyr Thr Phe 
        35                  40                  45              


Thr Asp Tyr Glu Met His Trp Val Lys Gln Thr Pro Val His Gly Leu 
    50                  55                  60                  


Lys Trp Ile Gly Ala Leu Asp Pro Lys Thr Gly Asp Thr Ala Tyr Ser 
65                  70                  75                  80  


Gln Lys Phe Lys Gly Lys Ala Thr Leu Thr Ala Asp Lys Ser Ser Ser 
                85                  90                  95      


Thr Ala Tyr Met Glu Leu Arg Ser Leu Thr Ser Glu Asp Ser Ala Val 
            100                 105                 110         


Tyr Tyr Cys Thr Arg Phe Tyr Ser Tyr Thr Tyr Trp Gly Gln Gly Thr 
        115                 120                 125             


Leu Val Thr Val Ser Ala Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser 
    130                 135                 140                 


Gly Gly Gly Gly Ser Asp Val Val Met Thr Gln Thr Pro Leu Ser Leu 
145                 150                 155                 160 


Pro Val Ser Leu Gly Asp Gln Ala Ser Ile Ser Cys Arg Ser Ser Gln 
                165                 170                 175     


Ser Leu Val His Ser Asn Gly Asn Thr Tyr Leu His Trp Tyr Leu Gln 
            180                 185                 190         


Lys Pro Gly Gln Ser Pro Lys Leu Leu Ile Tyr Lys Val Ser Asn Arg 
        195                 200                 205             


Phe Ser Gly Val Pro Asp Arg Phe Ser Gly Ser Gly Ser Gly Thr Asp 
    210                 215                 220                 


Phe Thr Leu Lys Ile Ser Arg Val Glu Ala Glu Asp Leu Gly Val Tyr 
225                 230                 235                 240 


Phe Cys Ser Gln Asn Thr His Val Pro Pro Thr Phe Gly Ser Gly Thr 
                245                 250                 255     


Lys Leu Glu Ile Lys Glu Pro Lys Ser Cys Asp Lys Thr His Thr Cys 
            260                 265                 270         


Pro Pro Cys Pro Asp Pro Lys Phe Trp Val Leu Val Val Val Gly Gly 
        275                 280                 285             


Val Leu Ala Cys Tyr Ser Leu Leu Val Thr Val Ala Phe Ile Ile Asn 
    290                 295                 300                 


Arg Arg Arg Arg Arg Glu Arg Arg Asp Leu Phe Thr Glu Ser Trp Asp 
305                 310                 315                 320 


Thr Gln Lys Ala Pro Asn Asn Tyr Arg Ser Pro Ile Ser Thr Ser Gln 
                325                 330                 335     


Pro Thr Asn Gln Ser Met Asp Asp Thr Arg Glu Asp Ile Tyr Val Asn 
            340                 345                 350         


Tyr Pro Thr Phe Ser Arg Arg Pro Lys Thr Arg Val Lys Arg Gly Arg 
        355                 360                 365             


Lys Lys Leu Leu Tyr Ile Phe Lys Gln Pro Phe Met Arg Pro Val Gln 
    370                 375                 380                 


Thr Thr Gln Glu Glu Asp Gly Cys Ser Cys Arg Phe Pro Glu Glu Glu 
385                 390                 395                 400 


Glu Gly Gly Cys Glu Leu Arg Val Lys Phe Ser Arg Ser Ala Asp Ala 
                405                 410                 415     


Pro Ala Tyr Gln Gln Gly Gln Asn Gln Leu Tyr Asn Glu Leu Asn Leu 
            420                 425                 430         


Gly Arg Arg Glu Glu Tyr Asp Val Leu Asp Lys Arg Arg Gly Arg Asp 
        435                 440                 445             


Pro Glu Met Gly Gly Lys Pro Arg Arg Lys Asn Pro Gln Glu Gly Leu 
    450                 455                 460                 


Tyr Asn Glu Leu Gln Lys Asp Lys Met Ala Glu Ala Tyr Ser Glu Ile 
465                 470                 475                 480 


Gly Met Lys Gly Glu Arg Arg Arg Gly Lys Gly His Asp Gly Leu Tyr 
                485                 490                 495     


Gln Gly Leu Ser Thr Ala Thr Lys Asp Thr Tyr Asp Ala Leu His Met 
            500                 505                 510         


Gln Ala Leu Pro Pro Arg 
        515             


<210>  65
<211>  520
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  hIgG heavy chain signal sequence/scFV GC33/hIgG1 hinge/CD28 TMD/ 
       CD226 ICD v2/41BB/CD3zeta

<400>  65

Met Asp Trp Ile Trp Arg Ile Leu Phe Leu Val Gly Ala Ala Thr Gly 
1               5                   10                  15      


Ala His Ser Gln Val Gln Leu Gln Gln Ser Gly Ala Glu Leu Val Arg 
            20                  25                  30          


Pro Gly Ala Ser Val Lys Leu Ser Cys Lys Ala Ser Gly Tyr Thr Phe 
        35                  40                  45              


Thr Asp Tyr Glu Met His Trp Val Lys Gln Thr Pro Val His Gly Leu 
    50                  55                  60                  


Lys Trp Ile Gly Ala Leu Asp Pro Lys Thr Gly Asp Thr Ala Tyr Ser 
65                  70                  75                  80  


Gln Lys Phe Lys Gly Lys Ala Thr Leu Thr Ala Asp Lys Ser Ser Ser 
                85                  90                  95      


Thr Ala Tyr Met Glu Leu Arg Ser Leu Thr Ser Glu Asp Ser Ala Val 
            100                 105                 110         


Tyr Tyr Cys Thr Arg Phe Tyr Ser Tyr Thr Tyr Trp Gly Gln Gly Thr 
        115                 120                 125             


Leu Val Thr Val Ser Ala Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser 
    130                 135                 140                 


Gly Gly Gly Gly Ser Asp Val Val Met Thr Gln Thr Pro Leu Ser Leu 
145                 150                 155                 160 


Pro Val Ser Leu Gly Asp Gln Ala Ser Ile Ser Cys Arg Ser Ser Gln 
                165                 170                 175     


Ser Leu Val His Ser Asn Gly Asn Thr Tyr Leu His Trp Tyr Leu Gln 
            180                 185                 190         


Lys Pro Gly Gln Ser Pro Lys Leu Leu Ile Tyr Lys Val Ser Asn Arg 
        195                 200                 205             


Phe Ser Gly Val Pro Asp Arg Phe Ser Gly Ser Gly Ser Gly Thr Asp 
    210                 215                 220                 


Phe Thr Leu Lys Ile Ser Arg Val Glu Ala Glu Asp Leu Gly Val Tyr 
225                 230                 235                 240 


Phe Cys Ser Gln Asn Thr His Val Pro Pro Thr Phe Gly Ser Gly Thr 
                245                 250                 255     


Lys Leu Glu Ile Lys Glu Pro Lys Ser Cys Asp Lys Thr His Thr Cys 
            260                 265                 270         


Pro Pro Cys Pro Asp Pro Lys Phe Trp Val Leu Val Val Val Gly Gly 
        275                 280                 285             


Val Leu Ala Cys Tyr Ser Leu Leu Val Thr Val Ala Phe Ile Ile Phe 
    290                 295                 300                 


Leu Asn Arg Arg Arg Arg Arg Glu Arg Arg Asp Leu Phe Thr Glu Ser 
305                 310                 315                 320 


Trp Asp Thr Gln Lys Ala Pro Asn Asn Tyr Arg Ser Pro Ile Ser Thr 
                325                 330                 335     


Ser Gln Pro Thr Asn Gln Ser Met Asp Asp Thr Arg Glu Asp Ile Tyr 
            340                 345                 350         


Val Asn Tyr Pro Thr Phe Ser Arg Arg Pro Lys Thr Arg Val Lys Arg 
        355                 360                 365             


Gly Arg Lys Lys Leu Leu Tyr Ile Phe Lys Gln Pro Phe Met Arg Pro 
    370                 375                 380                 


Val Gln Thr Thr Gln Glu Glu Asp Gly Cys Ser Cys Arg Phe Pro Glu 
385                 390                 395                 400 


Glu Glu Glu Gly Gly Cys Glu Leu Arg Val Lys Phe Ser Arg Ser Ala 
                405                 410                 415     


Asp Ala Pro Ala Tyr Gln Gln Gly Gln Asn Gln Leu Tyr Asn Glu Leu 
            420                 425                 430         


Asn Leu Gly Arg Arg Glu Glu Tyr Asp Val Leu Asp Lys Arg Arg Gly 
        435                 440                 445             


Arg Asp Pro Glu Met Gly Gly Lys Pro Arg Arg Lys Asn Pro Gln Glu 
    450                 455                 460                 


Gly Leu Tyr Asn Glu Leu Gln Lys Asp Lys Met Ala Glu Ala Tyr Ser 
465                 470                 475                 480 


Glu Ile Gly Met Lys Gly Glu Arg Arg Arg Gly Lys Gly His Asp Gly 
                485                 490                 495     


Leu Tyr Gln Gly Leu Ser Thr Ala Thr Lys Asp Thr Tyr Asp Ala Leu 
            500                 505                 510         


His Met Gln Ala Leu Pro Pro Arg 
        515                 520 


<210>  66
<211>  476
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  hIgG heavy chain signal sequence/scFV GC33/hIgG1 hinge/CD28 TMD/ 
       CD226 ICD v1/CD3zeta

<400>  66

Met Asp Trp Ile Trp Arg Ile Leu Phe Leu Val Gly Ala Ala Thr Gly 
1               5                   10                  15      


Ala His Ser Gln Val Gln Leu Gln Gln Ser Gly Ala Glu Leu Val Arg 
            20                  25                  30          


Pro Gly Ala Ser Val Lys Leu Ser Cys Lys Ala Ser Gly Tyr Thr Phe 
        35                  40                  45              


Thr Asp Tyr Glu Met His Trp Val Lys Gln Thr Pro Val His Gly Leu 
    50                  55                  60                  


Lys Trp Ile Gly Ala Leu Asp Pro Lys Thr Gly Asp Thr Ala Tyr Ser 
65                  70                  75                  80  


Gln Lys Phe Lys Gly Lys Ala Thr Leu Thr Ala Asp Lys Ser Ser Ser 
                85                  90                  95      


Thr Ala Tyr Met Glu Leu Arg Ser Leu Thr Ser Glu Asp Ser Ala Val 
            100                 105                 110         


Tyr Tyr Cys Thr Arg Phe Tyr Ser Tyr Thr Tyr Trp Gly Gln Gly Thr 
        115                 120                 125             


Leu Val Thr Val Ser Ala Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser 
    130                 135                 140                 


Gly Gly Gly Gly Ser Asp Val Val Met Thr Gln Thr Pro Leu Ser Leu 
145                 150                 155                 160 


Pro Val Ser Leu Gly Asp Gln Ala Ser Ile Ser Cys Arg Ser Ser Gln 
                165                 170                 175     


Ser Leu Val His Ser Asn Gly Asn Thr Tyr Leu His Trp Tyr Leu Gln 
            180                 185                 190         


Lys Pro Gly Gln Ser Pro Lys Leu Leu Ile Tyr Lys Val Ser Asn Arg 
        195                 200                 205             


Phe Ser Gly Val Pro Asp Arg Phe Ser Gly Ser Gly Ser Gly Thr Asp 
    210                 215                 220                 


Phe Thr Leu Lys Ile Ser Arg Val Glu Ala Glu Asp Leu Gly Val Tyr 
225                 230                 235                 240 


Phe Cys Ser Gln Asn Thr His Val Pro Pro Thr Phe Gly Ser Gly Thr 
                245                 250                 255     


Lys Leu Glu Ile Lys Glu Pro Lys Ser Cys Asp Lys Thr His Thr Cys 
            260                 265                 270         


Pro Pro Cys Pro Asp Pro Lys Phe Trp Val Leu Val Val Val Gly Gly 
        275                 280                 285             


Val Leu Ala Cys Tyr Ser Leu Leu Val Thr Val Ala Phe Ile Ile Asn 
    290                 295                 300                 


Arg Arg Arg Arg Arg Glu Arg Arg Asp Leu Phe Thr Glu Ser Trp Asp 
305                 310                 315                 320 


Thr Gln Lys Ala Pro Asn Asn Tyr Arg Ser Pro Ile Ser Thr Ser Gln 
                325                 330                 335     


Pro Thr Asn Gln Ser Met Asp Asp Thr Arg Glu Asp Ile Tyr Val Asn 
            340                 345                 350         


Tyr Pro Thr Phe Ser Arg Arg Pro Lys Thr Arg Val Arg Val Lys Phe 
        355                 360                 365             


Ser Arg Ser Ala Asp Ala Pro Ala Tyr Gln Gln Gly Gln Asn Gln Leu 
    370                 375                 380                 


Tyr Asn Glu Leu Asn Leu Gly Arg Arg Glu Glu Tyr Asp Val Leu Asp 
385                 390                 395                 400 


Lys Arg Arg Gly Arg Asp Pro Glu Met Gly Gly Lys Pro Arg Arg Lys 
                405                 410                 415     


Asn Pro Gln Glu Gly Leu Tyr Asn Glu Leu Gln Lys Asp Lys Met Ala 
            420                 425                 430         


Glu Ala Tyr Ser Glu Ile Gly Met Lys Gly Glu Arg Arg Arg Gly Lys 
        435                 440                 445             


Gly His Asp Gly Leu Tyr Gln Gly Leu Ser Thr Ala Thr Lys Asp Thr 
    450                 455                 460                 


Tyr Asp Ala Leu His Met Gln Ala Leu Pro Pro Arg 
465                 470                 475     


<210>  67
<211>  478
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  hIgG heavy chain signal sequence/scFV GC33/hIgG1 hinge/CD28 TMD/ 
       CD226 ICD v2/CD3zeta

<400>  67

Met Asp Trp Ile Trp Arg Ile Leu Phe Leu Val Gly Ala Ala Thr Gly 
1               5                   10                  15      


Ala His Ser Gln Val Gln Leu Gln Gln Ser Gly Ala Glu Leu Val Arg 
            20                  25                  30          


Pro Gly Ala Ser Val Lys Leu Ser Cys Lys Ala Ser Gly Tyr Thr Phe 
        35                  40                  45              


Thr Asp Tyr Glu Met His Trp Val Lys Gln Thr Pro Val His Gly Leu 
    50                  55                  60                  


Lys Trp Ile Gly Ala Leu Asp Pro Lys Thr Gly Asp Thr Ala Tyr Ser 
65                  70                  75                  80  


Gln Lys Phe Lys Gly Lys Ala Thr Leu Thr Ala Asp Lys Ser Ser Ser 
                85                  90                  95      


Thr Ala Tyr Met Glu Leu Arg Ser Leu Thr Ser Glu Asp Ser Ala Val 
            100                 105                 110         


Tyr Tyr Cys Thr Arg Phe Tyr Ser Tyr Thr Tyr Trp Gly Gln Gly Thr 
        115                 120                 125             


Leu Val Thr Val Ser Ala Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser 
    130                 135                 140                 


Gly Gly Gly Gly Ser Asp Val Val Met Thr Gln Thr Pro Leu Ser Leu 
145                 150                 155                 160 


Pro Val Ser Leu Gly Asp Gln Ala Ser Ile Ser Cys Arg Ser Ser Gln 
                165                 170                 175     


Ser Leu Val His Ser Asn Gly Asn Thr Tyr Leu His Trp Tyr Leu Gln 
            180                 185                 190         


Lys Pro Gly Gln Ser Pro Lys Leu Leu Ile Tyr Lys Val Ser Asn Arg 
        195                 200                 205             


Phe Ser Gly Val Pro Asp Arg Phe Ser Gly Ser Gly Ser Gly Thr Asp 
    210                 215                 220                 


Phe Thr Leu Lys Ile Ser Arg Val Glu Ala Glu Asp Leu Gly Val Tyr 
225                 230                 235                 240 


Phe Cys Ser Gln Asn Thr His Val Pro Pro Thr Phe Gly Ser Gly Thr 
                245                 250                 255     


Lys Leu Glu Ile Lys Glu Pro Lys Ser Cys Asp Lys Thr His Thr Cys 
            260                 265                 270         


Pro Pro Cys Pro Asp Pro Lys Phe Trp Val Leu Val Val Val Gly Gly 
        275                 280                 285             


Val Leu Ala Cys Tyr Ser Leu Leu Val Thr Val Ala Phe Ile Ile Phe 
    290                 295                 300                 


Leu Asn Arg Arg Arg Arg Arg Glu Arg Arg Asp Leu Phe Thr Glu Ser 
305                 310                 315                 320 


Trp Asp Thr Gln Lys Ala Pro Asn Asn Tyr Arg Ser Pro Ile Ser Thr 
                325                 330                 335     


Ser Gln Pro Thr Asn Gln Ser Met Asp Asp Thr Arg Glu Asp Ile Tyr 
            340                 345                 350         


Val Asn Tyr Pro Thr Phe Ser Arg Arg Pro Lys Thr Arg Val Arg Val 
        355                 360                 365             


Lys Phe Ser Arg Ser Ala Asp Ala Pro Ala Tyr Gln Gln Gly Gln Asn 
    370                 375                 380                 


Gln Leu Tyr Asn Glu Leu Asn Leu Gly Arg Arg Glu Glu Tyr Asp Val 
385                 390                 395                 400 


Leu Asp Lys Arg Arg Gly Arg Asp Pro Glu Met Gly Gly Lys Pro Arg 
                405                 410                 415     


Arg Lys Asn Pro Gln Glu Gly Leu Tyr Asn Glu Leu Gln Lys Asp Lys 
            420                 425                 430         


Met Ala Glu Ala Tyr Ser Glu Ile Gly Met Lys Gly Glu Arg Arg Arg 
        435                 440                 445             


Gly Lys Gly His Asp Gly Leu Tyr Gln Gly Leu Ser Thr Ala Thr Lys 
    450                 455                 460                 


Asp Thr Tyr Asp Ala Leu His Met Gln Ala Leu Pro Pro Arg 
465                 470                 475             


<210>  68
<211>  459
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  hIgG heavy chain signal sequence/scFV GC33/hIgG1 hinge/CD28 TMD/ 
       CD28 ICD/CD3zeta

<400>  68

Met Asp Trp Ile Trp Arg Ile Leu Phe Leu Val Gly Ala Ala Thr Gly 
1               5                   10                  15      


Ala His Ser Gln Val Gln Leu Gln Gln Ser Gly Ala Glu Leu Val Arg 
            20                  25                  30          


Pro Gly Ala Ser Val Lys Leu Ser Cys Lys Ala Ser Gly Tyr Thr Phe 
        35                  40                  45              


Thr Asp Tyr Glu Met His Trp Val Lys Gln Thr Pro Val His Gly Leu 
    50                  55                  60                  


Lys Trp Ile Gly Ala Leu Asp Pro Lys Thr Gly Asp Thr Ala Tyr Ser 
65                  70                  75                  80  


Gln Lys Phe Lys Gly Lys Ala Thr Leu Thr Ala Asp Lys Ser Ser Ser 
                85                  90                  95      


Thr Ala Tyr Met Glu Leu Arg Ser Leu Thr Ser Glu Asp Ser Ala Val 
            100                 105                 110         


Tyr Tyr Cys Thr Arg Phe Tyr Ser Tyr Thr Tyr Trp Gly Gln Gly Thr 
        115                 120                 125             


Leu Val Thr Val Ser Ala Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser 
    130                 135                 140                 


Gly Gly Gly Gly Ser Asp Val Val Met Thr Gln Thr Pro Leu Ser Leu 
145                 150                 155                 160 


Pro Val Ser Leu Gly Asp Gln Ala Ser Ile Ser Cys Arg Ser Ser Gln 
                165                 170                 175     


Ser Leu Val His Ser Asn Gly Asn Thr Tyr Leu His Trp Tyr Leu Gln 
            180                 185                 190         


Lys Pro Gly Gln Ser Pro Lys Leu Leu Ile Tyr Lys Val Ser Asn Arg 
        195                 200                 205             


Phe Ser Gly Val Pro Asp Arg Phe Ser Gly Ser Gly Ser Gly Thr Asp 
    210                 215                 220                 


Phe Thr Leu Lys Ile Ser Arg Val Glu Ala Glu Asp Leu Gly Val Tyr 
225                 230                 235                 240 


Phe Cys Ser Gln Asn Thr His Val Pro Pro Thr Phe Gly Ser Gly Thr 
                245                 250                 255     


Lys Leu Glu Ile Lys Glu Pro Lys Ser Cys Asp Lys Thr His Thr Cys 
            260                 265                 270         


Pro Pro Cys Pro Asp Pro Lys Phe Trp Val Leu Val Val Val Gly Gly 
        275                 280                 285             


Val Leu Ala Cys Tyr Ser Leu Leu Val Thr Val Ala Phe Ile Ile Phe 
    290                 295                 300                 


Trp Val Arg Ser Lys Arg Ser Arg Leu Leu His Ser Asp Tyr Met Asn 
305                 310                 315                 320 


Met Thr Pro Arg Arg Pro Gly Pro Thr Arg Lys His Tyr Gln Pro Tyr 
                325                 330                 335     


Ala Pro Pro Arg Asp Phe Ala Ala Tyr Arg Ser Arg Val Lys Phe Ser 
            340                 345                 350         


Arg Ser Ala Asp Ala Pro Ala Tyr Gln Gln Gly Gln Asn Gln Leu Tyr 
        355                 360                 365             


Asn Glu Leu Asn Leu Gly Arg Arg Glu Glu Tyr Asp Val Leu Asp Lys 
    370                 375                 380                 


Arg Arg Gly Arg Asp Pro Glu Met Gly Gly Lys Pro Arg Arg Lys Asn 
385                 390                 395                 400 


Pro Gln Glu Gly Leu Tyr Asn Glu Leu Gln Lys Asp Lys Met Ala Glu 
                405                 410                 415     


Ala Tyr Ser Glu Ile Gly Met Lys Gly Glu Arg Arg Arg Gly Lys Gly 
            420                 425                 430         


His Asp Gly Leu Tyr Gln Gly Leu Ser Thr Ala Thr Lys Asp Thr Tyr 
        435                 440                 445             


Asp Ala Leu His Met Gln Ala Leu Pro Pro Arg 
    450                 455                 


<210>  69
<211>  520
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  hIgG heavy chain signal sequence/scFV GC33/hIgG1 hinge/CD28 TMD/ 
       CD226 ICD v1/ CD28 ICD/CD3zeta

<400>  69

Met Asp Trp Ile Trp Arg Ile Leu Phe Leu Val Gly Ala Ala Thr Gly 
1               5                   10                  15      


Ala His Ser Gln Val Gln Leu Gln Gln Ser Gly Ala Glu Leu Val Arg 
            20                  25                  30          


Pro Gly Ala Ser Val Lys Leu Ser Cys Lys Ala Ser Gly Tyr Thr Phe 
        35                  40                  45              


Thr Asp Tyr Glu Met His Trp Val Lys Gln Thr Pro Val His Gly Leu 
    50                  55                  60                  


Lys Trp Ile Gly Ala Leu Asp Pro Lys Thr Gly Asp Thr Ala Tyr Ser 
65                  70                  75                  80  


Gln Lys Phe Lys Gly Lys Ala Thr Leu Thr Ala Asp Lys Ser Ser Ser 
                85                  90                  95      


Thr Ala Tyr Met Glu Leu Arg Ser Leu Thr Ser Glu Asp Ser Ala Val 
            100                 105                 110         


Tyr Tyr Cys Thr Arg Phe Tyr Ser Tyr Thr Tyr Trp Gly Gln Gly Thr 
        115                 120                 125             


Leu Val Thr Val Ser Ala Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser 
    130                 135                 140                 


Gly Gly Gly Gly Ser Asp Val Val Met Thr Gln Thr Pro Leu Ser Leu 
145                 150                 155                 160 


Pro Val Ser Leu Gly Asp Gln Ala Ser Ile Ser Cys Arg Ser Ser Gln 
                165                 170                 175     


Ser Leu Val His Ser Asn Gly Asn Thr Tyr Leu His Trp Tyr Leu Gln 
            180                 185                 190         


Lys Pro Gly Gln Ser Pro Lys Leu Leu Ile Tyr Lys Val Ser Asn Arg 
        195                 200                 205             


Phe Ser Gly Val Pro Asp Arg Phe Ser Gly Ser Gly Ser Gly Thr Asp 
    210                 215                 220                 


Phe Thr Leu Lys Ile Ser Arg Val Glu Ala Glu Asp Leu Gly Val Tyr 
225                 230                 235                 240 


Phe Cys Ser Gln Asn Thr His Val Pro Pro Thr Phe Gly Ser Gly Thr 
                245                 250                 255     


Lys Leu Glu Ile Lys Glu Pro Lys Ser Cys Asp Lys Thr His Thr Cys 
            260                 265                 270         


Pro Pro Cys Pro Asp Pro Lys Phe Trp Val Leu Val Val Val Gly Gly 
        275                 280                 285             


Val Leu Ala Cys Tyr Ser Leu Leu Val Thr Val Ala Phe Ile Ile Asn 
    290                 295                 300                 


Arg Arg Arg Arg Arg Glu Arg Arg Asp Leu Phe Thr Glu Ser Trp Asp 
305                 310                 315                 320 


Thr Gln Lys Ala Pro Asn Asn Tyr Arg Ser Pro Ile Ser Thr Ser Gln 
                325                 330                 335     


Pro Thr Asn Gln Ser Met Asp Asp Thr Arg Glu Asp Ile Tyr Val Asn 
            340                 345                 350         


Tyr Pro Thr Phe Ser Arg Arg Pro Lys Thr Arg Val Phe Trp Val Arg 
        355                 360                 365             


Ser Lys Arg Ser Arg Leu Leu His Ser Asp Tyr Met Asn Met Thr Pro 
    370                 375                 380                 


Arg Arg Pro Gly Pro Thr Arg Lys His Tyr Gln Pro Tyr Ala Pro Pro 
385                 390                 395                 400 


Arg Asp Phe Ala Ala Tyr Arg Ser Arg Val Lys Phe Ser Arg Ser Ala 
                405                 410                 415     


Asp Ala Pro Ala Tyr Gln Gln Gly Gln Asn Gln Leu Tyr Asn Glu Leu 
            420                 425                 430         


Asn Leu Gly Arg Arg Glu Glu Tyr Asp Val Leu Asp Lys Arg Arg Gly 
        435                 440                 445             


Arg Asp Pro Glu Met Gly Gly Lys Pro Arg Arg Lys Asn Pro Gln Glu 
    450                 455                 460                 


Gly Leu Tyr Asn Glu Leu Gln Lys Asp Lys Met Ala Glu Ala Tyr Ser 
465                 470                 475                 480 


Glu Ile Gly Met Lys Gly Glu Arg Arg Arg Gly Lys Gly His Asp Gly 
                485                 490                 495     


Leu Tyr Gln Gly Leu Ser Thr Ala Thr Lys Asp Thr Tyr Asp Ala Leu 
            500                 505                 510         


His Met Gln Ala Leu Pro Pro Arg 
        515                 520 


<210>  70
<211>  522
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  hIgG heavy chain signal sequence/scFV GC33/hIgG1 hinge/CD28 TMD/ 
       CD226 ICD v2/ CD28 ICD/CD3zeta

<400>  70

Met Asp Trp Ile Trp Arg Ile Leu Phe Leu Val Gly Ala Ala Thr Gly 
1               5                   10                  15      


Ala His Ser Gln Val Gln Leu Gln Gln Ser Gly Ala Glu Leu Val Arg 
            20                  25                  30          


Pro Gly Ala Ser Val Lys Leu Ser Cys Lys Ala Ser Gly Tyr Thr Phe 
        35                  40                  45              


Thr Asp Tyr Glu Met His Trp Val Lys Gln Thr Pro Val His Gly Leu 
    50                  55                  60                  


Lys Trp Ile Gly Ala Leu Asp Pro Lys Thr Gly Asp Thr Ala Tyr Ser 
65                  70                  75                  80  


Gln Lys Phe Lys Gly Lys Ala Thr Leu Thr Ala Asp Lys Ser Ser Ser 
                85                  90                  95      


Thr Ala Tyr Met Glu Leu Arg Ser Leu Thr Ser Glu Asp Ser Ala Val 
            100                 105                 110         


Tyr Tyr Cys Thr Arg Phe Tyr Ser Tyr Thr Tyr Trp Gly Gln Gly Thr 
        115                 120                 125             


Leu Val Thr Val Ser Ala Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser 
    130                 135                 140                 


Gly Gly Gly Gly Ser Asp Val Val Met Thr Gln Thr Pro Leu Ser Leu 
145                 150                 155                 160 


Pro Val Ser Leu Gly Asp Gln Ala Ser Ile Ser Cys Arg Ser Ser Gln 
                165                 170                 175     


Ser Leu Val His Ser Asn Gly Asn Thr Tyr Leu His Trp Tyr Leu Gln 
            180                 185                 190         


Lys Pro Gly Gln Ser Pro Lys Leu Leu Ile Tyr Lys Val Ser Asn Arg 
        195                 200                 205             


Phe Ser Gly Val Pro Asp Arg Phe Ser Gly Ser Gly Ser Gly Thr Asp 
    210                 215                 220                 


Phe Thr Leu Lys Ile Ser Arg Val Glu Ala Glu Asp Leu Gly Val Tyr 
225                 230                 235                 240 


Phe Cys Ser Gln Asn Thr His Val Pro Pro Thr Phe Gly Ser Gly Thr 
                245                 250                 255     


Lys Leu Glu Ile Lys Glu Pro Lys Ser Cys Asp Lys Thr His Thr Cys 
            260                 265                 270         


Pro Pro Cys Pro Asp Pro Lys Phe Trp Val Leu Val Val Val Gly Gly 
        275                 280                 285             


Val Leu Ala Cys Tyr Ser Leu Leu Val Thr Val Ala Phe Ile Ile Phe 
    290                 295                 300                 


Leu Asn Arg Arg Arg Arg Arg Glu Arg Arg Asp Leu Phe Thr Glu Ser 
305                 310                 315                 320 


Trp Asp Thr Gln Lys Ala Pro Asn Asn Tyr Arg Ser Pro Ile Ser Thr 
                325                 330                 335     


Ser Gln Pro Thr Asn Gln Ser Met Asp Asp Thr Arg Glu Asp Ile Tyr 
            340                 345                 350         


Val Asn Tyr Pro Thr Phe Ser Arg Arg Pro Lys Thr Arg Val Phe Trp 
        355                 360                 365             


Val Arg Ser Lys Arg Ser Arg Leu Leu His Ser Asp Tyr Met Asn Met 
    370                 375                 380                 


Thr Pro Arg Arg Pro Gly Pro Thr Arg Lys His Tyr Gln Pro Tyr Ala 
385                 390                 395                 400 


Pro Pro Arg Asp Phe Ala Ala Tyr Arg Ser Arg Val Lys Phe Ser Arg 
                405                 410                 415     


Ser Ala Asp Ala Pro Ala Tyr Gln Gln Gly Gln Asn Gln Leu Tyr Asn 
            420                 425                 430         


Glu Leu Asn Leu Gly Arg Arg Glu Glu Tyr Asp Val Leu Asp Lys Arg 
        435                 440                 445             


Arg Gly Arg Asp Pro Glu Met Gly Gly Lys Pro Arg Arg Lys Asn Pro 
    450                 455                 460                 


Gln Glu Gly Leu Tyr Asn Glu Leu Gln Lys Asp Lys Met Ala Glu Ala 
465                 470                 475                 480 


Tyr Ser Glu Ile Gly Met Lys Gly Glu Arg Arg Arg Gly Lys Gly His 
                485                 490                 495     


Asp Gly Leu Tyr Gln Gly Leu Ser Thr Ala Thr Lys Asp Thr Tyr Asp 
            500                 505                 510         


Ala Leu His Met Gln Ala Leu Pro Pro Arg 
        515                 520         


<210>  71
<211>  520
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  hIgG heavy chain signal sequence/scFV GC33/hIgG1 hinge/CD28 TMD/ 
       CD28 ICD/CD226 ICD v1/CD3zeta

<400>  71

Met Asp Trp Ile Trp Arg Ile Leu Phe Leu Val Gly Ala Ala Thr Gly 
1               5                   10                  15      


Ala His Ser Gln Val Gln Leu Gln Gln Ser Gly Ala Glu Leu Val Arg 
            20                  25                  30          


Pro Gly Ala Ser Val Lys Leu Ser Cys Lys Ala Ser Gly Tyr Thr Phe 
        35                  40                  45              


Thr Asp Tyr Glu Met His Trp Val Lys Gln Thr Pro Val His Gly Leu 
    50                  55                  60                  


Lys Trp Ile Gly Ala Leu Asp Pro Lys Thr Gly Asp Thr Ala Tyr Ser 
65                  70                  75                  80  


Gln Lys Phe Lys Gly Lys Ala Thr Leu Thr Ala Asp Lys Ser Ser Ser 
                85                  90                  95      


Thr Ala Tyr Met Glu Leu Arg Ser Leu Thr Ser Glu Asp Ser Ala Val 
            100                 105                 110         


Tyr Tyr Cys Thr Arg Phe Tyr Ser Tyr Thr Tyr Trp Gly Gln Gly Thr 
        115                 120                 125             


Leu Val Thr Val Ser Ala Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser 
    130                 135                 140                 


Gly Gly Gly Gly Ser Asp Val Val Met Thr Gln Thr Pro Leu Ser Leu 
145                 150                 155                 160 


Pro Val Ser Leu Gly Asp Gln Ala Ser Ile Ser Cys Arg Ser Ser Gln 
                165                 170                 175     


Ser Leu Val His Ser Asn Gly Asn Thr Tyr Leu His Trp Tyr Leu Gln 
            180                 185                 190         


Lys Pro Gly Gln Ser Pro Lys Leu Leu Ile Tyr Lys Val Ser Asn Arg 
        195                 200                 205             


Phe Ser Gly Val Pro Asp Arg Phe Ser Gly Ser Gly Ser Gly Thr Asp 
    210                 215                 220                 


Phe Thr Leu Lys Ile Ser Arg Val Glu Ala Glu Asp Leu Gly Val Tyr 
225                 230                 235                 240 


Phe Cys Ser Gln Asn Thr His Val Pro Pro Thr Phe Gly Ser Gly Thr 
                245                 250                 255     


Lys Leu Glu Ile Lys Glu Pro Lys Ser Cys Asp Lys Thr His Thr Cys 
            260                 265                 270         


Pro Pro Cys Pro Asp Pro Lys Phe Trp Val Leu Val Val Val Gly Gly 
        275                 280                 285             


Val Leu Ala Cys Tyr Ser Leu Leu Val Thr Val Ala Phe Ile Ile Phe 
    290                 295                 300                 


Trp Val Arg Ser Lys Arg Ser Arg Leu Leu His Ser Asp Tyr Met Asn 
305                 310                 315                 320 


Met Thr Pro Arg Arg Pro Gly Pro Thr Arg Lys His Tyr Gln Pro Tyr 
                325                 330                 335     


Ala Pro Pro Arg Asp Phe Ala Ala Tyr Arg Ser Asn Arg Arg Arg Arg 
            340                 345                 350         


Arg Glu Arg Arg Asp Leu Phe Thr Glu Ser Trp Asp Thr Gln Lys Ala 
        355                 360                 365             


Pro Asn Asn Tyr Arg Ser Pro Ile Ser Thr Ser Gln Pro Thr Asn Gln 
    370                 375                 380                 


Ser Met Asp Asp Thr Arg Glu Asp Ile Tyr Val Asn Tyr Pro Thr Phe 
385                 390                 395                 400 


Ser Arg Arg Pro Lys Thr Arg Val Arg Val Lys Phe Ser Arg Ser Ala 
                405                 410                 415     


Asp Ala Pro Ala Tyr Gln Gln Gly Gln Asn Gln Leu Tyr Asn Glu Leu 
            420                 425                 430         


Asn Leu Gly Arg Arg Glu Glu Tyr Asp Val Leu Asp Lys Arg Arg Gly 
        435                 440                 445             


Arg Asp Pro Glu Met Gly Gly Lys Pro Arg Arg Lys Asn Pro Gln Glu 
    450                 455                 460                 


Gly Leu Tyr Asn Glu Leu Gln Lys Asp Lys Met Ala Glu Ala Tyr Ser 
465                 470                 475                 480 


Glu Ile Gly Met Lys Gly Glu Arg Arg Arg Gly Lys Gly His Asp Gly 
                485                 490                 495     


Leu Tyr Gln Gly Leu Ser Thr Ala Thr Lys Asp Thr Tyr Asp Ala Leu 
            500                 505                 510         


His Met Gln Ala Leu Pro Pro Arg 
        515                 520 


<210>  72
<211>  501
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  hIgG heavy chain signal sequence/scFV GC33/hIgG1 hinge/CD28 TMD/ 
       CD28 ICD/41BB/CD3zeta

<400>  72

Met Asp Trp Ile Trp Arg Ile Leu Phe Leu Val Gly Ala Ala Thr Gly 
1               5                   10                  15      


Ala His Ser Gln Val Gln Leu Gln Gln Ser Gly Ala Glu Leu Val Arg 
            20                  25                  30          


Pro Gly Ala Ser Val Lys Leu Ser Cys Lys Ala Ser Gly Tyr Thr Phe 
        35                  40                  45              


Thr Asp Tyr Glu Met His Trp Val Lys Gln Thr Pro Val His Gly Leu 
    50                  55                  60                  


Lys Trp Ile Gly Ala Leu Asp Pro Lys Thr Gly Asp Thr Ala Tyr Ser 
65                  70                  75                  80  


Gln Lys Phe Lys Gly Lys Ala Thr Leu Thr Ala Asp Lys Ser Ser Ser 
                85                  90                  95      


Thr Ala Tyr Met Glu Leu Arg Ser Leu Thr Ser Glu Asp Ser Ala Val 
            100                 105                 110         


Tyr Tyr Cys Thr Arg Phe Tyr Ser Tyr Thr Tyr Trp Gly Gln Gly Thr 
        115                 120                 125             


Leu Val Thr Val Ser Ala Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser 
    130                 135                 140                 


Gly Gly Gly Gly Ser Asp Val Val Met Thr Gln Thr Pro Leu Ser Leu 
145                 150                 155                 160 


Pro Val Ser Leu Gly Asp Gln Ala Ser Ile Ser Cys Arg Ser Ser Gln 
                165                 170                 175     


Ser Leu Val His Ser Asn Gly Asn Thr Tyr Leu His Trp Tyr Leu Gln 
            180                 185                 190         


Lys Pro Gly Gln Ser Pro Lys Leu Leu Ile Tyr Lys Val Ser Asn Arg 
        195                 200                 205             


Phe Ser Gly Val Pro Asp Arg Phe Ser Gly Ser Gly Ser Gly Thr Asp 
    210                 215                 220                 


Phe Thr Leu Lys Ile Ser Arg Val Glu Ala Glu Asp Leu Gly Val Tyr 
225                 230                 235                 240 


Phe Cys Ser Gln Asn Thr His Val Pro Pro Thr Phe Gly Ser Gly Thr 
                245                 250                 255     


Lys Leu Glu Ile Lys Glu Pro Lys Ser Cys Asp Lys Thr His Thr Cys 
            260                 265                 270         


Pro Pro Cys Pro Asp Pro Lys Phe Trp Val Leu Val Val Val Gly Gly 
        275                 280                 285             


Val Leu Ala Cys Tyr Ser Leu Leu Val Thr Val Ala Phe Ile Ile Phe 
    290                 295                 300                 


Trp Val Arg Ser Lys Arg Ser Arg Leu Leu His Ser Asp Tyr Met Asn 
305                 310                 315                 320 


Met Thr Pro Arg Arg Pro Gly Pro Thr Arg Lys His Tyr Gln Pro Tyr 
                325                 330                 335     


Ala Pro Pro Arg Asp Phe Ala Ala Tyr Arg Ser Lys Arg Gly Arg Lys 
            340                 345                 350         


Lys Leu Leu Tyr Ile Phe Lys Gln Pro Phe Met Arg Pro Val Gln Thr 
        355                 360                 365             


Thr Gln Glu Glu Asp Gly Cys Ser Cys Arg Phe Pro Glu Glu Glu Glu 
    370                 375                 380                 


Gly Gly Cys Glu Leu Arg Val Lys Phe Ser Arg Ser Ala Asp Ala Pro 
385                 390                 395                 400 


Ala Tyr Gln Gln Gly Gln Asn Gln Leu Tyr Asn Glu Leu Asn Leu Gly 
                405                 410                 415     


Arg Arg Glu Glu Tyr Asp Val Leu Asp Lys Arg Arg Gly Arg Asp Pro 
            420                 425                 430         


Glu Met Gly Gly Lys Pro Arg Arg Lys Asn Pro Gln Glu Gly Leu Tyr 
        435                 440                 445             


Asn Glu Leu Gln Lys Asp Lys Met Ala Glu Ala Tyr Ser Glu Ile Gly 
    450                 455                 460                 


Met Lys Gly Glu Arg Arg Arg Gly Lys Gly His Asp Gly Leu Tyr Gln 
465                 470                 475                 480 


Gly Leu Ser Thr Ala Thr Lys Asp Thr Tyr Asp Ala Leu His Met Gln 
                485                 490                 495     


Ala Leu Pro Pro Arg 
            500     


<210>  73
<211>  562
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  hIgG heavy chain signal sequence/scFV GC33/hIgG1 hinge/CD28 TMD/ 
       CD226 ICD v1/ CD28 ICD/41BB/CD3zeta

<400>  73

Met Asp Trp Ile Trp Arg Ile Leu Phe Leu Val Gly Ala Ala Thr Gly 
1               5                   10                  15      


Ala His Ser Gln Val Gln Leu Gln Gln Ser Gly Ala Glu Leu Val Arg 
            20                  25                  30          


Pro Gly Ala Ser Val Lys Leu Ser Cys Lys Ala Ser Gly Tyr Thr Phe 
        35                  40                  45              


Thr Asp Tyr Glu Met His Trp Val Lys Gln Thr Pro Val His Gly Leu 
    50                  55                  60                  


Lys Trp Ile Gly Ala Leu Asp Pro Lys Thr Gly Asp Thr Ala Tyr Ser 
65                  70                  75                  80  


Gln Lys Phe Lys Gly Lys Ala Thr Leu Thr Ala Asp Lys Ser Ser Ser 
                85                  90                  95      


Thr Ala Tyr Met Glu Leu Arg Ser Leu Thr Ser Glu Asp Ser Ala Val 
            100                 105                 110         


Tyr Tyr Cys Thr Arg Phe Tyr Ser Tyr Thr Tyr Trp Gly Gln Gly Thr 
        115                 120                 125             


Leu Val Thr Val Ser Ala Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser 
    130                 135                 140                 


Gly Gly Gly Gly Ser Asp Val Val Met Thr Gln Thr Pro Leu Ser Leu 
145                 150                 155                 160 


Pro Val Ser Leu Gly Asp Gln Ala Ser Ile Ser Cys Arg Ser Ser Gln 
                165                 170                 175     


Ser Leu Val His Ser Asn Gly Asn Thr Tyr Leu His Trp Tyr Leu Gln 
            180                 185                 190         


Lys Pro Gly Gln Ser Pro Lys Leu Leu Ile Tyr Lys Val Ser Asn Arg 
        195                 200                 205             


Phe Ser Gly Val Pro Asp Arg Phe Ser Gly Ser Gly Ser Gly Thr Asp 
    210                 215                 220                 


Phe Thr Leu Lys Ile Ser Arg Val Glu Ala Glu Asp Leu Gly Val Tyr 
225                 230                 235                 240 


Phe Cys Ser Gln Asn Thr His Val Pro Pro Thr Phe Gly Ser Gly Thr 
                245                 250                 255     


Lys Leu Glu Ile Lys Glu Pro Lys Ser Cys Asp Lys Thr His Thr Cys 
            260                 265                 270         


Pro Pro Cys Pro Asp Pro Lys Phe Trp Val Leu Val Val Val Gly Gly 
        275                 280                 285             


Val Leu Ala Cys Tyr Ser Leu Leu Val Thr Val Ala Phe Ile Ile Asn 
    290                 295                 300                 


Arg Arg Arg Arg Arg Glu Arg Arg Asp Leu Phe Thr Glu Ser Trp Asp 
305                 310                 315                 320 


Thr Gln Lys Ala Pro Asn Asn Tyr Arg Ser Pro Ile Ser Thr Ser Gln 
                325                 330                 335     


Pro Thr Asn Gln Ser Met Asp Asp Thr Arg Glu Asp Ile Tyr Val Asn 
            340                 345                 350         


Tyr Pro Thr Phe Ser Arg Arg Pro Lys Thr Arg Val Phe Trp Val Arg 
        355                 360                 365             


Ser Lys Arg Ser Arg Leu Leu His Ser Asp Tyr Met Asn Met Thr Pro 
    370                 375                 380                 


Arg Arg Pro Gly Pro Thr Arg Lys His Tyr Gln Pro Tyr Ala Pro Pro 
385                 390                 395                 400 


Arg Asp Phe Ala Ala Tyr Arg Ser Lys Arg Gly Arg Lys Lys Leu Leu 
                405                 410                 415     


Tyr Ile Phe Lys Gln Pro Phe Met Arg Pro Val Gln Thr Thr Gln Glu 
            420                 425                 430         


Glu Asp Gly Cys Ser Cys Arg Phe Pro Glu Glu Glu Glu Gly Gly Cys 
        435                 440                 445             


Glu Leu Arg Val Lys Phe Ser Arg Ser Ala Asp Ala Pro Ala Tyr Gln 
    450                 455                 460                 


Gln Gly Gln Asn Gln Leu Tyr Asn Glu Leu Asn Leu Gly Arg Arg Glu 
465                 470                 475                 480 


Glu Tyr Asp Val Leu Asp Lys Arg Arg Gly Arg Asp Pro Glu Met Gly 
                485                 490                 495     


Gly Lys Pro Arg Arg Lys Asn Pro Gln Glu Gly Leu Tyr Asn Glu Leu 
            500                 505                 510         


Gln Lys Asp Lys Met Ala Glu Ala Tyr Ser Glu Ile Gly Met Lys Gly 
        515                 520                 525             


Glu Arg Arg Arg Gly Lys Gly His Asp Gly Leu Tyr Gln Gly Leu Ser 
    530                 535                 540                 


Thr Ala Thr Lys Asp Thr Tyr Asp Ala Leu His Met Gln Ala Leu Pro 
545                 550                 555                 560 


Pro Arg 
        


<210>  74
<211>  564
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  hIgG heavy chain signal sequence/scFV GC33/hIgG1 hinge/CD28 TMD/ 
       CD226 ICD v2/ CD28 ICD/41BB/CD3zeta

<400>  74

Met Asp Trp Ile Trp Arg Ile Leu Phe Leu Val Gly Ala Ala Thr Gly 
1               5                   10                  15      


Ala His Ser Gln Val Gln Leu Gln Gln Ser Gly Ala Glu Leu Val Arg 
            20                  25                  30          


Pro Gly Ala Ser Val Lys Leu Ser Cys Lys Ala Ser Gly Tyr Thr Phe 
        35                  40                  45              


Thr Asp Tyr Glu Met His Trp Val Lys Gln Thr Pro Val His Gly Leu 
    50                  55                  60                  


Lys Trp Ile Gly Ala Leu Asp Pro Lys Thr Gly Asp Thr Ala Tyr Ser 
65                  70                  75                  80  


Gln Lys Phe Lys Gly Lys Ala Thr Leu Thr Ala Asp Lys Ser Ser Ser 
                85                  90                  95      


Thr Ala Tyr Met Glu Leu Arg Ser Leu Thr Ser Glu Asp Ser Ala Val 
            100                 105                 110         


Tyr Tyr Cys Thr Arg Phe Tyr Ser Tyr Thr Tyr Trp Gly Gln Gly Thr 
        115                 120                 125             


Leu Val Thr Val Ser Ala Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser 
    130                 135                 140                 


Gly Gly Gly Gly Ser Asp Val Val Met Thr Gln Thr Pro Leu Ser Leu 
145                 150                 155                 160 


Pro Val Ser Leu Gly Asp Gln Ala Ser Ile Ser Cys Arg Ser Ser Gln 
                165                 170                 175     


Ser Leu Val His Ser Asn Gly Asn Thr Tyr Leu His Trp Tyr Leu Gln 
            180                 185                 190         


Lys Pro Gly Gln Ser Pro Lys Leu Leu Ile Tyr Lys Val Ser Asn Arg 
        195                 200                 205             


Phe Ser Gly Val Pro Asp Arg Phe Ser Gly Ser Gly Ser Gly Thr Asp 
    210                 215                 220                 


Phe Thr Leu Lys Ile Ser Arg Val Glu Ala Glu Asp Leu Gly Val Tyr 
225                 230                 235                 240 


Phe Cys Ser Gln Asn Thr His Val Pro Pro Thr Phe Gly Ser Gly Thr 
                245                 250                 255     


Lys Leu Glu Ile Lys Glu Pro Lys Ser Cys Asp Lys Thr His Thr Cys 
            260                 265                 270         


Pro Pro Cys Pro Asp Pro Lys Phe Trp Val Leu Val Val Val Gly Gly 
        275                 280                 285             


Val Leu Ala Cys Tyr Ser Leu Leu Val Thr Val Ala Phe Ile Ile Phe 
    290                 295                 300                 


Leu Asn Arg Arg Arg Arg Arg Glu Arg Arg Asp Leu Phe Thr Glu Ser 
305                 310                 315                 320 


Trp Asp Thr Gln Lys Ala Pro Asn Asn Tyr Arg Ser Pro Ile Ser Thr 
                325                 330                 335     


Ser Gln Pro Thr Asn Gln Ser Met Asp Asp Thr Arg Glu Asp Ile Tyr 
            340                 345                 350         


Val Asn Tyr Pro Thr Phe Ser Arg Arg Pro Lys Thr Arg Val Phe Trp 
        355                 360                 365             


Val Arg Ser Lys Arg Ser Arg Leu Leu His Ser Asp Tyr Met Asn Met 
    370                 375                 380                 


Thr Pro Arg Arg Pro Gly Pro Thr Arg Lys His Tyr Gln Pro Tyr Ala 
385                 390                 395                 400 


Pro Pro Arg Asp Phe Ala Ala Tyr Arg Ser Lys Arg Gly Arg Lys Lys 
                405                 410                 415     


Leu Leu Tyr Ile Phe Lys Gln Pro Phe Met Arg Pro Val Gln Thr Thr 
            420                 425                 430         


Gln Glu Glu Asp Gly Cys Ser Cys Arg Phe Pro Glu Glu Glu Glu Gly 
        435                 440                 445             


Gly Cys Glu Leu Arg Val Lys Phe Ser Arg Ser Ala Asp Ala Pro Ala 
    450                 455                 460                 


Tyr Gln Gln Gly Gln Asn Gln Leu Tyr Asn Glu Leu Asn Leu Gly Arg 
465                 470                 475                 480 


Arg Glu Glu Tyr Asp Val Leu Asp Lys Arg Arg Gly Arg Asp Pro Glu 
                485                 490                 495     


Met Gly Gly Lys Pro Arg Arg Lys Asn Pro Gln Glu Gly Leu Tyr Asn 
            500                 505                 510         


Glu Leu Gln Lys Asp Lys Met Ala Glu Ala Tyr Ser Glu Ile Gly Met 
        515                 520                 525             


Lys Gly Glu Arg Arg Arg Gly Lys Gly His Asp Gly Leu Tyr Gln Gly 
    530                 535                 540                 


Leu Ser Thr Ala Thr Lys Asp Thr Tyr Asp Ala Leu His Met Gln Ala 
545                 550                 555                 560 


Leu Pro Pro Arg 
                


<210>  75
<211>  562
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  hIgG heavy chain signal sequence/scFV GC33/hIgG1 hinge/CD28 TMD/ 
       CD28 ICD/CD226 ICD v1/41BB/CD3zeta

<400>  75

Met Asp Trp Ile Trp Arg Ile Leu Phe Leu Val Gly Ala Ala Thr Gly 
1               5                   10                  15      


Ala His Ser Gln Val Gln Leu Gln Gln Ser Gly Ala Glu Leu Val Arg 
            20                  25                  30          


Pro Gly Ala Ser Val Lys Leu Ser Cys Lys Ala Ser Gly Tyr Thr Phe 
        35                  40                  45              


Thr Asp Tyr Glu Met His Trp Val Lys Gln Thr Pro Val His Gly Leu 
    50                  55                  60                  


Lys Trp Ile Gly Ala Leu Asp Pro Lys Thr Gly Asp Thr Ala Tyr Ser 
65                  70                  75                  80  


Gln Lys Phe Lys Gly Lys Ala Thr Leu Thr Ala Asp Lys Ser Ser Ser 
                85                  90                  95      


Thr Ala Tyr Met Glu Leu Arg Ser Leu Thr Ser Glu Asp Ser Ala Val 
            100                 105                 110         


Tyr Tyr Cys Thr Arg Phe Tyr Ser Tyr Thr Tyr Trp Gly Gln Gly Thr 
        115                 120                 125             


Leu Val Thr Val Ser Ala Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser 
    130                 135                 140                 


Gly Gly Gly Gly Ser Asp Val Val Met Thr Gln Thr Pro Leu Ser Leu 
145                 150                 155                 160 


Pro Val Ser Leu Gly Asp Gln Ala Ser Ile Ser Cys Arg Ser Ser Gln 
                165                 170                 175     


Ser Leu Val His Ser Asn Gly Asn Thr Tyr Leu His Trp Tyr Leu Gln 
            180                 185                 190         


Lys Pro Gly Gln Ser Pro Lys Leu Leu Ile Tyr Lys Val Ser Asn Arg 
        195                 200                 205             


Phe Ser Gly Val Pro Asp Arg Phe Ser Gly Ser Gly Ser Gly Thr Asp 
    210                 215                 220                 


Phe Thr Leu Lys Ile Ser Arg Val Glu Ala Glu Asp Leu Gly Val Tyr 
225                 230                 235                 240 


Phe Cys Ser Gln Asn Thr His Val Pro Pro Thr Phe Gly Ser Gly Thr 
                245                 250                 255     


Lys Leu Glu Ile Lys Glu Pro Lys Ser Cys Asp Lys Thr His Thr Cys 
            260                 265                 270         


Pro Pro Cys Pro Asp Pro Lys Phe Trp Val Leu Val Val Val Gly Gly 
        275                 280                 285             


Val Leu Ala Cys Tyr Ser Leu Leu Val Thr Val Ala Phe Ile Ile Phe 
    290                 295                 300                 


Trp Val Arg Ser Lys Arg Ser Arg Leu Leu His Ser Asp Tyr Met Asn 
305                 310                 315                 320 


Met Thr Pro Arg Arg Pro Gly Pro Thr Arg Lys His Tyr Gln Pro Tyr 
                325                 330                 335     


Ala Pro Pro Arg Asp Phe Ala Ala Tyr Arg Ser Asn Arg Arg Arg Arg 
            340                 345                 350         


Arg Glu Arg Arg Asp Leu Phe Thr Glu Ser Trp Asp Thr Gln Lys Ala 
        355                 360                 365             


Pro Asn Asn Tyr Arg Ser Pro Ile Ser Thr Ser Gln Pro Thr Asn Gln 
    370                 375                 380                 


Ser Met Asp Asp Thr Arg Glu Asp Ile Tyr Val Asn Tyr Pro Thr Phe 
385                 390                 395                 400 


Ser Arg Arg Pro Lys Thr Arg Val Lys Arg Gly Arg Lys Lys Leu Leu 
                405                 410                 415     


Tyr Ile Phe Lys Gln Pro Phe Met Arg Pro Val Gln Thr Thr Gln Glu 
            420                 425                 430         


Glu Asp Gly Cys Ser Cys Arg Phe Pro Glu Glu Glu Glu Gly Gly Cys 
        435                 440                 445             


Glu Leu Arg Val Lys Phe Ser Arg Ser Ala Asp Ala Pro Ala Tyr Gln 
    450                 455                 460                 


Gln Gly Gln Asn Gln Leu Tyr Asn Glu Leu Asn Leu Gly Arg Arg Glu 
465                 470                 475                 480 


Glu Tyr Asp Val Leu Asp Lys Arg Arg Gly Arg Asp Pro Glu Met Gly 
                485                 490                 495     


Gly Lys Pro Arg Arg Lys Asn Pro Gln Glu Gly Leu Tyr Asn Glu Leu 
            500                 505                 510         


Gln Lys Asp Lys Met Ala Glu Ala Tyr Ser Glu Ile Gly Met Lys Gly 
        515                 520                 525             


Glu Arg Arg Arg Gly Lys Gly His Asp Gly Leu Tyr Gln Gly Leu Ser 
    530                 535                 540                 


Thr Ala Thr Lys Asp Thr Tyr Asp Ala Leu His Met Gln Ala Leu Pro 
545                 550                 555                 560 


Pro Arg 
        


<210>  76
<211>  515
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  hIgG heavy chain signal sequence/scFV GC33/hIgG1 hinge/CD226 
       TMD/CD226 ICD v1/41BB/CD3zeta

<400>  76

Met Asp Trp Ile Trp Arg Ile Leu Phe Leu Val Gly Ala Ala Thr Gly 
1               5                   10                  15      


Ala His Ser Gln Val Gln Leu Gln Gln Ser Gly Ala Glu Leu Val Arg 
            20                  25                  30          


Pro Gly Ala Ser Val Lys Leu Ser Cys Lys Ala Ser Gly Tyr Thr Phe 
        35                  40                  45              


Thr Asp Tyr Glu Met His Trp Val Lys Gln Thr Pro Val His Gly Leu 
    50                  55                  60                  


Lys Trp Ile Gly Ala Leu Asp Pro Lys Thr Gly Asp Thr Ala Tyr Ser 
65                  70                  75                  80  


Gln Lys Phe Lys Gly Lys Ala Thr Leu Thr Ala Asp Lys Ser Ser Ser 
                85                  90                  95      


Thr Ala Tyr Met Glu Leu Arg Ser Leu Thr Ser Glu Asp Ser Ala Val 
            100                 105                 110         


Tyr Tyr Cys Thr Arg Phe Tyr Ser Tyr Thr Tyr Trp Gly Gln Gly Thr 
        115                 120                 125             


Leu Val Thr Val Ser Ala Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser 
    130                 135                 140                 


Gly Gly Gly Gly Ser Asp Val Val Met Thr Gln Thr Pro Leu Ser Leu 
145                 150                 155                 160 


Pro Val Ser Leu Gly Asp Gln Ala Ser Ile Ser Cys Arg Ser Ser Gln 
                165                 170                 175     


Ser Leu Val His Ser Asn Gly Asn Thr Tyr Leu His Trp Tyr Leu Gln 
            180                 185                 190         


Lys Pro Gly Gln Ser Pro Lys Leu Leu Ile Tyr Lys Val Ser Asn Arg 
        195                 200                 205             


Phe Ser Gly Val Pro Asp Arg Phe Ser Gly Ser Gly Ser Gly Thr Asp 
    210                 215                 220                 


Phe Thr Leu Lys Ile Ser Arg Val Glu Ala Glu Asp Leu Gly Val Tyr 
225                 230                 235                 240 


Phe Cys Ser Gln Asn Thr His Val Pro Pro Thr Phe Gly Ser Gly Thr 
                245                 250                 255     


Lys Leu Glu Ile Lys Glu Pro Lys Ser Cys Asp Lys Thr His Thr Cys 
            260                 265                 270         


Pro Pro Cys Pro Asp Pro Lys Gly Gly Thr Val Leu Leu Leu Leu Phe 
        275                 280                 285             


Val Ile Ser Ile Thr Thr Ile Ile Val Ile Phe Leu Asn Arg Arg Arg 
    290                 295                 300                 


Arg Arg Glu Arg Arg Asp Leu Phe Thr Glu Ser Trp Asp Thr Gln Lys 
305                 310                 315                 320 


Ala Pro Asn Asn Tyr Arg Ser Pro Ile Ser Thr Ser Gln Pro Thr Asn 
                325                 330                 335     


Gln Ser Met Asp Asp Thr Arg Glu Asp Ile Tyr Val Asn Tyr Pro Thr 
            340                 345                 350         


Phe Ser Arg Arg Pro Lys Thr Arg Val Lys Arg Gly Arg Lys Lys Leu 
        355                 360                 365             


Leu Tyr Ile Phe Lys Gln Pro Phe Met Arg Pro Val Gln Thr Thr Gln 
    370                 375                 380                 


Glu Glu Asp Gly Cys Ser Cys Arg Phe Pro Glu Glu Glu Glu Gly Gly 
385                 390                 395                 400 


Cys Glu Leu Arg Val Lys Phe Ser Arg Ser Ala Asp Ala Pro Ala Tyr 
                405                 410                 415     


Gln Gln Gly Gln Asn Gln Leu Tyr Asn Glu Leu Asn Leu Gly Arg Arg 
            420                 425                 430         


Glu Glu Tyr Asp Val Leu Asp Lys Arg Arg Gly Arg Asp Pro Glu Met 
        435                 440                 445             


Gly Gly Lys Pro Arg Arg Lys Asn Pro Gln Glu Gly Leu Tyr Asn Glu 
    450                 455                 460                 


Leu Gln Lys Asp Lys Met Ala Glu Ala Tyr Ser Glu Ile Gly Met Lys 
465                 470                 475                 480 


Gly Glu Arg Arg Arg Gly Lys Gly His Asp Gly Leu Tyr Gln Gly Leu 
                485                 490                 495     


Ser Thr Ala Thr Lys Asp Thr Tyr Asp Ala Leu His Met Gln Ala Leu 
            500                 505                 510         


Pro Pro Arg 
        515 


<210>  77
<211>  364
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  hIgG heavy chain signal sequence/scFV 3-17I/hIgG1 hinge/CD226 
       TMD/CD226 ICD v1

<400>  77

Met Asp Trp Ile Trp Arg Ile Leu Phe Leu Val Gly Ala Ala Thr Gly 
1               5                   10                  15      


Ala His Ser Gln Val Gln Leu Val Gln Ser Gly Ala Glu Val Lys Lys 
            20                  25                  30          


Pro Gly Ser Ser Val Lys Val Ser Cys Lys Ala Ser Gly Gly Thr Phe 
        35                  40                  45              


Ser Ser Tyr Ala Ile Ser Trp Val Arg Gln Ala Pro Gly Gln Gly Leu 
    50                  55                  60                  


Glu Trp Met Gly Gly Ile Ile Pro Ile Phe Gly Thr Ala Asn Tyr Ala 
65                  70                  75                  80  


Gln Lys Phe Gln Gly Arg Val Thr Ile Thr Ala Asp Glu Ser Thr Ser 
                85                  90                  95      


Thr Ala Tyr Met Glu Leu Ser Ser Leu Arg Ser Glu Asp Thr Ala Val 
            100                 105                 110         


Tyr Tyr Cys Ala Arg Gly Leu Leu Trp Asn Tyr Trp Gly Gln Gly Thr 
        115                 120                 125             


Leu Val Thr Val Ser Ser Lys Leu Ser Gly Ser Ala Ser Ala Pro Lys 
    130                 135                 140                 


Leu Glu Glu Gly Glu Phe Ser Glu Ala Arg Val Glu Ile Val Met Thr 
145                 150                 155                 160 


Gln Ser Pro Ala Thr Leu Ser Val Ser Pro Gly Glu Arg Ala Thr Leu 
                165                 170                 175     


Ser Cys Arg Ala Ser Gln Ser Val Ser Ser Asn Leu Ala Trp Tyr Gln 
            180                 185                 190         


Gln Lys Pro Gly Gln Ala Pro Arg Leu Ile Ile Tyr Gly Ala Ser Thr 
        195                 200                 205             


Thr Ala Ser Gly Ile Pro Ala Arg Phe Ser Ala Ser Gly Ser Gly Thr 
    210                 215                 220                 


Asp Phe Thr Leu Thr Ile Ser Ser Leu Gln Ser Glu Asp Phe Ala Val 
225                 230                 235                 240 


Tyr Tyr Cys Gln Gln Tyr Asn Asn Trp Pro Pro Ala Tyr Thr Phe Gly 
                245                 250                 255     


Gln Gly Thr Lys Leu Glu Ile Lys Glu Pro Lys Ser Cys Asp Lys Thr 
            260                 265                 270         


His Thr Cys Pro Pro Cys Pro Asp Pro Lys Gly Gly Thr Val Leu Leu 
        275                 280                 285             


Leu Leu Phe Val Ile Ser Ile Thr Thr Ile Ile Val Ile Phe Leu Asn 
    290                 295                 300                 


Arg Arg Arg Arg Arg Glu Arg Arg Asp Leu Phe Thr Glu Ser Trp Asp 
305                 310                 315                 320 


Thr Gln Lys Ala Pro Asn Asn Tyr Arg Ser Pro Ile Ser Thr Ser Gln 
                325                 330                 335     


Pro Thr Asn Gln Ser Met Asp Asp Thr Arg Glu Asp Ile Tyr Val Asn 
            340                 345                 350         


Tyr Pro Thr Phe Ser Arg Arg Pro Lys Thr Arg Val 
        355                 360                 


<210>  78
<211>  415
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  hIgG heavy chain signal sequence/scFV 3-17I/hIgG1 hinge/CD226 
       TMD/ CD3zeta

<400>  78

Met Asp Trp Ile Trp Arg Ile Leu Phe Leu Val Gly Ala Ala Thr Gly 
1               5                   10                  15      


Ala His Ser Gln Val Gln Leu Val Gln Ser Gly Ala Glu Val Lys Lys 
            20                  25                  30          


Pro Gly Ser Ser Val Lys Val Ser Cys Lys Ala Ser Gly Gly Thr Phe 
        35                  40                  45              


Ser Ser Tyr Ala Ile Ser Trp Val Arg Gln Ala Pro Gly Gln Gly Leu 
    50                  55                  60                  


Glu Trp Met Gly Gly Ile Ile Pro Ile Phe Gly Thr Ala Asn Tyr Ala 
65                  70                  75                  80  


Gln Lys Phe Gln Gly Arg Val Thr Ile Thr Ala Asp Glu Ser Thr Ser 
                85                  90                  95      


Thr Ala Tyr Met Glu Leu Ser Ser Leu Arg Ser Glu Asp Thr Ala Val 
            100                 105                 110         


Tyr Tyr Cys Ala Arg Gly Leu Leu Trp Asn Tyr Trp Gly Gln Gly Thr 
        115                 120                 125             


Leu Val Thr Val Ser Ser Lys Leu Ser Gly Ser Ala Ser Ala Pro Lys 
    130                 135                 140                 


Leu Glu Glu Gly Glu Phe Ser Glu Ala Arg Val Glu Ile Val Met Thr 
145                 150                 155                 160 


Gln Ser Pro Ala Thr Leu Ser Val Ser Pro Gly Glu Arg Ala Thr Leu 
                165                 170                 175     


Ser Cys Arg Ala Ser Gln Ser Val Ser Ser Asn Leu Ala Trp Tyr Gln 
            180                 185                 190         


Gln Lys Pro Gly Gln Ala Pro Arg Leu Ile Ile Tyr Gly Ala Ser Thr 
        195                 200                 205             


Thr Ala Ser Gly Ile Pro Ala Arg Phe Ser Ala Ser Gly Ser Gly Thr 
    210                 215                 220                 


Asp Phe Thr Leu Thr Ile Ser Ser Leu Gln Ser Glu Asp Phe Ala Val 
225                 230                 235                 240 


Tyr Tyr Cys Gln Gln Tyr Asn Asn Trp Pro Pro Ala Tyr Thr Phe Gly 
                245                 250                 255     


Gln Gly Thr Lys Leu Glu Ile Lys Glu Pro Lys Ser Cys Asp Lys Thr 
            260                 265                 270         


His Thr Cys Pro Pro Cys Pro Asp Pro Lys Gly Gly Thr Val Leu Leu 
        275                 280                 285             


Leu Leu Phe Val Ile Ser Ile Thr Thr Ile Ile Val Ile Phe Leu Arg 
    290                 295                 300                 


Val Lys Phe Ser Arg Ser Ala Asp Ala Pro Ala Tyr Gln Gln Gly Gln 
305                 310                 315                 320 


Asn Gln Leu Tyr Asn Glu Leu Asn Leu Gly Arg Arg Glu Glu Tyr Asp 
                325                 330                 335     


Val Leu Asp Lys Arg Arg Gly Arg Asp Pro Glu Met Gly Gly Lys Pro 
            340                 345                 350         


Arg Arg Lys Asn Pro Gln Glu Gly Leu Tyr Asn Glu Leu Gln Lys Asp 
        355                 360                 365             


Lys Met Ala Glu Ala Tyr Ser Glu Ile Gly Met Lys Gly Glu Arg Arg 
    370                 375                 380                 


Arg Gly Lys Gly His Asp Gly Leu Tyr Gln Gly Leu Ser Thr Ala Thr 
385                 390                 395                 400 


Lys Asp Thr Tyr Asp Ala Leu His Met Gln Ala Leu Pro Pro Arg 
                405                 410                 415 


<210>  79
<211>  476
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  hIgG heavy chain signal sequence/scFV 3-17I/hIgG1 hinge/CD226 
       TMD/CD226 ICD v1/CD3zeta

<400>  79

Met Asp Trp Ile Trp Arg Ile Leu Phe Leu Val Gly Ala Ala Thr Gly 
1               5                   10                  15      


Ala His Ser Gln Val Gln Leu Val Gln Ser Gly Ala Glu Val Lys Lys 
            20                  25                  30          


Pro Gly Ser Ser Val Lys Val Ser Cys Lys Ala Ser Gly Gly Thr Phe 
        35                  40                  45              


Ser Ser Tyr Ala Ile Ser Trp Val Arg Gln Ala Pro Gly Gln Gly Leu 
    50                  55                  60                  


Glu Trp Met Gly Gly Ile Ile Pro Ile Phe Gly Thr Ala Asn Tyr Ala 
65                  70                  75                  80  


Gln Lys Phe Gln Gly Arg Val Thr Ile Thr Ala Asp Glu Ser Thr Ser 
                85                  90                  95      


Thr Ala Tyr Met Glu Leu Ser Ser Leu Arg Ser Glu Asp Thr Ala Val 
            100                 105                 110         


Tyr Tyr Cys Ala Arg Gly Leu Leu Trp Asn Tyr Trp Gly Gln Gly Thr 
        115                 120                 125             


Leu Val Thr Val Ser Ser Lys Leu Ser Gly Ser Ala Ser Ala Pro Lys 
    130                 135                 140                 


Leu Glu Glu Gly Glu Phe Ser Glu Ala Arg Val Glu Ile Val Met Thr 
145                 150                 155                 160 


Gln Ser Pro Ala Thr Leu Ser Val Ser Pro Gly Glu Arg Ala Thr Leu 
                165                 170                 175     


Ser Cys Arg Ala Ser Gln Ser Val Ser Ser Asn Leu Ala Trp Tyr Gln 
            180                 185                 190         


Gln Lys Pro Gly Gln Ala Pro Arg Leu Ile Ile Tyr Gly Ala Ser Thr 
        195                 200                 205             


Thr Ala Ser Gly Ile Pro Ala Arg Phe Ser Ala Ser Gly Ser Gly Thr 
    210                 215                 220                 


Asp Phe Thr Leu Thr Ile Ser Ser Leu Gln Ser Glu Asp Phe Ala Val 
225                 230                 235                 240 


Tyr Tyr Cys Gln Gln Tyr Asn Asn Trp Pro Pro Ala Tyr Thr Phe Gly 
                245                 250                 255     


Gln Gly Thr Lys Leu Glu Ile Lys Glu Pro Lys Ser Cys Asp Lys Thr 
            260                 265                 270         


His Thr Cys Pro Pro Cys Pro Asp Pro Lys Gly Gly Thr Val Leu Leu 
        275                 280                 285             


Leu Leu Phe Val Ile Ser Ile Thr Thr Ile Ile Val Ile Phe Leu Asn 
    290                 295                 300                 


Arg Arg Arg Arg Arg Glu Arg Arg Asp Leu Phe Thr Glu Ser Trp Asp 
305                 310                 315                 320 


Thr Gln Lys Ala Pro Asn Asn Tyr Arg Ser Pro Ile Ser Thr Ser Gln 
                325                 330                 335     


Pro Thr Asn Gln Ser Met Asp Asp Thr Arg Glu Asp Ile Tyr Val Asn 
            340                 345                 350         


Tyr Pro Thr Phe Ser Arg Arg Pro Lys Thr Arg Val Arg Val Lys Phe 
        355                 360                 365             


Ser Arg Ser Ala Asp Ala Pro Ala Tyr Gln Gln Gly Gln Asn Gln Leu 
    370                 375                 380                 


Tyr Asn Glu Leu Asn Leu Gly Arg Arg Glu Glu Tyr Asp Val Leu Asp 
385                 390                 395                 400 


Lys Arg Arg Gly Arg Asp Pro Glu Met Gly Gly Lys Pro Arg Arg Lys 
                405                 410                 415     


Asn Pro Gln Glu Gly Leu Tyr Asn Glu Leu Gln Lys Asp Lys Met Ala 
            420                 425                 430         


Glu Ala Tyr Ser Glu Ile Gly Met Lys Gly Glu Arg Arg Arg Gly Lys 
        435                 440                 445             


Gly His Asp Gly Leu Tyr Gln Gly Leu Ser Thr Ala Thr Lys Asp Thr 
    450                 455                 460                 


Tyr Asp Ala Leu His Met Gln Ala Leu Pro Pro Arg 
465                 470                 475     


<210>  80
<211>  396
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  scFV GC33/hIgG1 hinge/CD28 TMD/CD3zeta

<400>  80

Gln Val Gln Leu Gln Gln Ser Gly Ala Glu Leu Val Arg Pro Gly Ala 
1               5                   10                  15      


Ser Val Lys Leu Ser Cys Lys Ala Ser Gly Tyr Thr Phe Thr Asp Tyr 
            20                  25                  30          


Glu Met His Trp Val Lys Gln Thr Pro Val His Gly Leu Lys Trp Ile 
        35                  40                  45              


Gly Ala Leu Asp Pro Lys Thr Gly Asp Thr Ala Tyr Ser Gln Lys Phe 
    50                  55                  60                  


Lys Gly Lys Ala Thr Leu Thr Ala Asp Lys Ser Ser Ser Thr Ala Tyr 
65                  70                  75                  80  


Met Glu Leu Arg Ser Leu Thr Ser Glu Asp Ser Ala Val Tyr Tyr Cys 
                85                  90                  95      


Thr Arg Phe Tyr Ser Tyr Thr Tyr Trp Gly Gln Gly Thr Leu Val Thr 
            100                 105                 110         


Val Ser Ala Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly 
        115                 120                 125             


Gly Ser Asp Val Val Met Thr Gln Thr Pro Leu Ser Leu Pro Val Ser 
    130                 135                 140                 


Leu Gly Asp Gln Ala Ser Ile Ser Cys Arg Ser Ser Gln Ser Leu Val 
145                 150                 155                 160 


His Ser Asn Gly Asn Thr Tyr Leu His Trp Tyr Leu Gln Lys Pro Gly 
                165                 170                 175     


Gln Ser Pro Lys Leu Leu Ile Tyr Lys Val Ser Asn Arg Phe Ser Gly 
            180                 185                 190         


Val Pro Asp Arg Phe Ser Gly Ser Gly Ser Gly Thr Asp Phe Thr Leu 
        195                 200                 205             


Lys Ile Ser Arg Val Glu Ala Glu Asp Leu Gly Val Tyr Phe Cys Ser 
    210                 215                 220                 


Gln Asn Thr His Val Pro Pro Thr Phe Gly Ser Gly Thr Lys Leu Glu 
225                 230                 235                 240 


Ile Lys Glu Pro Lys Ser Cys Asp Lys Thr His Thr Cys Pro Pro Cys 
                245                 250                 255     


Pro Asp Pro Lys Phe Trp Val Leu Val Val Val Gly Gly Val Leu Ala 
            260                 265                 270         


Cys Tyr Ser Leu Leu Val Thr Val Ala Phe Ile Ile Arg Val Lys Phe 
        275                 280                 285             


Ser Arg Ser Ala Asp Ala Pro Ala Tyr Gln Gln Gly Gln Asn Gln Leu 
    290                 295                 300                 


Tyr Asn Glu Leu Asn Leu Gly Arg Arg Glu Glu Tyr Asp Val Leu Asp 
305                 310                 315                 320 


Lys Arg Arg Gly Arg Asp Pro Glu Met Gly Gly Lys Pro Arg Arg Lys 
                325                 330                 335     


Asn Pro Gln Glu Gly Leu Tyr Asn Glu Leu Gln Lys Asp Lys Met Ala 
            340                 345                 350         


Glu Ala Tyr Ser Glu Ile Gly Met Lys Gly Glu Arg Arg Arg Gly Lys 
        355                 360                 365             


Gly His Asp Gly Leu Tyr Gln Gly Leu Ser Thr Ala Thr Lys Asp Thr 
    370                 375                 380                 


Tyr Asp Ala Leu His Met Gln Ala Leu Pro Pro Arg 
385                 390                 395     


<210>  81
<211>  347
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  scFV GC33/hIgG1 hinge/CD28 TMD/CD226 ICD v2

<400>  81

Gln Val Gln Leu Gln Gln Ser Gly Ala Glu Leu Val Arg Pro Gly Ala 
1               5                   10                  15      


Ser Val Lys Leu Ser Cys Lys Ala Ser Gly Tyr Thr Phe Thr Asp Tyr 
            20                  25                  30          


Glu Met His Trp Val Lys Gln Thr Pro Val His Gly Leu Lys Trp Ile 
        35                  40                  45              


Gly Ala Leu Asp Pro Lys Thr Gly Asp Thr Ala Tyr Ser Gln Lys Phe 
    50                  55                  60                  


Lys Gly Lys Ala Thr Leu Thr Ala Asp Lys Ser Ser Ser Thr Ala Tyr 
65                  70                  75                  80  


Met Glu Leu Arg Ser Leu Thr Ser Glu Asp Ser Ala Val Tyr Tyr Cys 
                85                  90                  95      


Thr Arg Phe Tyr Ser Tyr Thr Tyr Trp Gly Gln Gly Thr Leu Val Thr 
            100                 105                 110         


Val Ser Ala Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly 
        115                 120                 125             


Gly Ser Asp Val Val Met Thr Gln Thr Pro Leu Ser Leu Pro Val Ser 
    130                 135                 140                 


Leu Gly Asp Gln Ala Ser Ile Ser Cys Arg Ser Ser Gln Ser Leu Val 
145                 150                 155                 160 


His Ser Asn Gly Asn Thr Tyr Leu His Trp Tyr Leu Gln Lys Pro Gly 
                165                 170                 175     


Gln Ser Pro Lys Leu Leu Ile Tyr Lys Val Ser Asn Arg Phe Ser Gly 
            180                 185                 190         


Val Pro Asp Arg Phe Ser Gly Ser Gly Ser Gly Thr Asp Phe Thr Leu 
        195                 200                 205             


Lys Ile Ser Arg Val Glu Ala Glu Asp Leu Gly Val Tyr Phe Cys Ser 
    210                 215                 220                 


Gln Asn Thr His Val Pro Pro Thr Phe Gly Ser Gly Thr Lys Leu Glu 
225                 230                 235                 240 


Ile Lys Glu Pro Lys Ser Cys Asp Lys Thr His Thr Cys Pro Pro Cys 
                245                 250                 255     


Pro Asp Pro Lys Phe Trp Val Leu Val Val Val Gly Gly Val Leu Ala 
            260                 265                 270         


Cys Tyr Ser Leu Leu Val Thr Val Ala Phe Ile Ile Phe Leu Asn Arg 
        275                 280                 285             


Arg Arg Arg Arg Glu Arg Arg Asp Leu Phe Thr Glu Ser Trp Asp Thr 
    290                 295                 300                 


Gln Lys Ala Pro Asn Asn Tyr Arg Ser Pro Ile Ser Thr Ser Gln Pro 
305                 310                 315                 320 


Thr Asn Gln Ser Met Asp Asp Thr Arg Glu Asp Ile Tyr Val Asn Tyr 
                325                 330                 335     


Pro Thr Phe Ser Arg Arg Pro Lys Thr Arg Val 
            340                 345         


<210>  82
<211>  438
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  scFV GC33/hIgG1 hinge/CD28 TMD/41BB/CD3zeta

<400>  82

Gln Val Gln Leu Gln Gln Ser Gly Ala Glu Leu Val Arg Pro Gly Ala 
1               5                   10                  15      


Ser Val Lys Leu Ser Cys Lys Ala Ser Gly Tyr Thr Phe Thr Asp Tyr 
            20                  25                  30          


Glu Met His Trp Val Lys Gln Thr Pro Val His Gly Leu Lys Trp Ile 
        35                  40                  45              


Gly Ala Leu Asp Pro Lys Thr Gly Asp Thr Ala Tyr Ser Gln Lys Phe 
    50                  55                  60                  


Lys Gly Lys Ala Thr Leu Thr Ala Asp Lys Ser Ser Ser Thr Ala Tyr 
65                  70                  75                  80  


Met Glu Leu Arg Ser Leu Thr Ser Glu Asp Ser Ala Val Tyr Tyr Cys 
                85                  90                  95      


Thr Arg Phe Tyr Ser Tyr Thr Tyr Trp Gly Gln Gly Thr Leu Val Thr 
            100                 105                 110         


Val Ser Ala Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly 
        115                 120                 125             


Gly Ser Asp Val Val Met Thr Gln Thr Pro Leu Ser Leu Pro Val Ser 
    130                 135                 140                 


Leu Gly Asp Gln Ala Ser Ile Ser Cys Arg Ser Ser Gln Ser Leu Val 
145                 150                 155                 160 


His Ser Asn Gly Asn Thr Tyr Leu His Trp Tyr Leu Gln Lys Pro Gly 
                165                 170                 175     


Gln Ser Pro Lys Leu Leu Ile Tyr Lys Val Ser Asn Arg Phe Ser Gly 
            180                 185                 190         


Val Pro Asp Arg Phe Ser Gly Ser Gly Ser Gly Thr Asp Phe Thr Leu 
        195                 200                 205             


Lys Ile Ser Arg Val Glu Ala Glu Asp Leu Gly Val Tyr Phe Cys Ser 
    210                 215                 220                 


Gln Asn Thr His Val Pro Pro Thr Phe Gly Ser Gly Thr Lys Leu Glu 
225                 230                 235                 240 


Ile Lys Glu Pro Lys Ser Cys Asp Lys Thr His Thr Cys Pro Pro Cys 
                245                 250                 255     


Pro Asp Pro Lys Phe Trp Val Leu Val Val Val Gly Gly Val Leu Ala 
            260                 265                 270         


Cys Tyr Ser Leu Leu Val Thr Val Ala Phe Ile Ile Lys Arg Gly Arg 
        275                 280                 285             


Lys Lys Leu Leu Tyr Ile Phe Lys Gln Pro Phe Met Arg Pro Val Gln 
    290                 295                 300                 


Thr Thr Gln Glu Glu Asp Gly Cys Ser Cys Arg Phe Pro Glu Glu Glu 
305                 310                 315                 320 


Glu Gly Gly Cys Glu Leu Arg Val Lys Phe Ser Arg Ser Ala Asp Ala 
                325                 330                 335     


Pro Ala Tyr Gln Gln Gly Gln Asn Gln Leu Tyr Asn Glu Leu Asn Leu 
            340                 345                 350         


Gly Arg Arg Glu Glu Tyr Asp Val Leu Asp Lys Arg Arg Gly Arg Asp 
        355                 360                 365             


Pro Glu Met Gly Gly Lys Pro Arg Arg Lys Asn Pro Gln Glu Gly Leu 
    370                 375                 380                 


Tyr Asn Glu Leu Gln Lys Asp Lys Met Ala Glu Ala Tyr Ser Glu Ile 
385                 390                 395                 400 


Gly Met Lys Gly Glu Arg Arg Arg Gly Lys Gly His Asp Gly Leu Tyr 
                405                 410                 415     


Gln Gly Leu Ser Thr Ala Thr Lys Asp Thr Tyr Asp Ala Leu His Met 
            420                 425                 430         


Gln Ala Leu Pro Pro Arg 
        435             


<210>  83
<211>  499
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  scFV GC33/hIgG1 hinge/CD28 TMD/ CD226 ICD v1/41BB/CD3zeta

<400>  83

Gln Val Gln Leu Gln Gln Ser Gly Ala Glu Leu Val Arg Pro Gly Ala 
1               5                   10                  15      


Ser Val Lys Leu Ser Cys Lys Ala Ser Gly Tyr Thr Phe Thr Asp Tyr 
            20                  25                  30          


Glu Met His Trp Val Lys Gln Thr Pro Val His Gly Leu Lys Trp Ile 
        35                  40                  45              


Gly Ala Leu Asp Pro Lys Thr Gly Asp Thr Ala Tyr Ser Gln Lys Phe 
    50                  55                  60                  


Lys Gly Lys Ala Thr Leu Thr Ala Asp Lys Ser Ser Ser Thr Ala Tyr 
65                  70                  75                  80  


Met Glu Leu Arg Ser Leu Thr Ser Glu Asp Ser Ala Val Tyr Tyr Cys 
                85                  90                  95      


Thr Arg Phe Tyr Ser Tyr Thr Tyr Trp Gly Gln Gly Thr Leu Val Thr 
            100                 105                 110         


Val Ser Ala Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly 
        115                 120                 125             


Gly Ser Asp Val Val Met Thr Gln Thr Pro Leu Ser Leu Pro Val Ser 
    130                 135                 140                 


Leu Gly Asp Gln Ala Ser Ile Ser Cys Arg Ser Ser Gln Ser Leu Val 
145                 150                 155                 160 


His Ser Asn Gly Asn Thr Tyr Leu His Trp Tyr Leu Gln Lys Pro Gly 
                165                 170                 175     


Gln Ser Pro Lys Leu Leu Ile Tyr Lys Val Ser Asn Arg Phe Ser Gly 
            180                 185                 190         


Val Pro Asp Arg Phe Ser Gly Ser Gly Ser Gly Thr Asp Phe Thr Leu 
        195                 200                 205             


Lys Ile Ser Arg Val Glu Ala Glu Asp Leu Gly Val Tyr Phe Cys Ser 
    210                 215                 220                 


Gln Asn Thr His Val Pro Pro Thr Phe Gly Ser Gly Thr Lys Leu Glu 
225                 230                 235                 240 


Ile Lys Glu Pro Lys Ser Cys Asp Lys Thr His Thr Cys Pro Pro Cys 
                245                 250                 255     


Pro Asp Pro Lys Phe Trp Val Leu Val Val Val Gly Gly Val Leu Ala 
            260                 265                 270         


Cys Tyr Ser Leu Leu Val Thr Val Ala Phe Ile Ile Asn Arg Arg Arg 
        275                 280                 285             


Arg Arg Glu Arg Arg Asp Leu Phe Thr Glu Ser Trp Asp Thr Gln Lys 
    290                 295                 300                 


Ala Pro Asn Asn Tyr Arg Ser Pro Ile Ser Thr Ser Gln Pro Thr Asn 
305                 310                 315                 320 


Gln Ser Met Asp Asp Thr Arg Glu Asp Ile Tyr Val Asn Tyr Pro Thr 
                325                 330                 335     


Phe Ser Arg Arg Pro Lys Thr Arg Val Lys Arg Gly Arg Lys Lys Leu 
            340                 345                 350         


Leu Tyr Ile Phe Lys Gln Pro Phe Met Arg Pro Val Gln Thr Thr Gln 
        355                 360                 365             


Glu Glu Asp Gly Cys Ser Cys Arg Phe Pro Glu Glu Glu Glu Gly Gly 
    370                 375                 380                 


Cys Glu Leu Arg Val Lys Phe Ser Arg Ser Ala Asp Ala Pro Ala Tyr 
385                 390                 395                 400 


Gln Gln Gly Gln Asn Gln Leu Tyr Asn Glu Leu Asn Leu Gly Arg Arg 
                405                 410                 415     


Glu Glu Tyr Asp Val Leu Asp Lys Arg Arg Gly Arg Asp Pro Glu Met 
            420                 425                 430         


Gly Gly Lys Pro Arg Arg Lys Asn Pro Gln Glu Gly Leu Tyr Asn Glu 
        435                 440                 445             


Leu Gln Lys Asp Lys Met Ala Glu Ala Tyr Ser Glu Ile Gly Met Lys 
    450                 455                 460                 


Gly Glu Arg Arg Arg Gly Lys Gly His Asp Gly Leu Tyr Gln Gly Leu 
465                 470                 475                 480 


Ser Thr Ala Thr Lys Asp Thr Tyr Asp Ala Leu His Met Gln Ala Leu 
                485                 490                 495     


Pro Pro Arg 
            


<210>  84
<211>  501
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  scFV GC33/hIgG1 hinge/CD28 TMD/ CD226 ICD v2/41BB/CD3zeta

<400>  84

Gln Val Gln Leu Gln Gln Ser Gly Ala Glu Leu Val Arg Pro Gly Ala 
1               5                   10                  15      


Ser Val Lys Leu Ser Cys Lys Ala Ser Gly Tyr Thr Phe Thr Asp Tyr 
            20                  25                  30          


Glu Met His Trp Val Lys Gln Thr Pro Val His Gly Leu Lys Trp Ile 
        35                  40                  45              


Gly Ala Leu Asp Pro Lys Thr Gly Asp Thr Ala Tyr Ser Gln Lys Phe 
    50                  55                  60                  


Lys Gly Lys Ala Thr Leu Thr Ala Asp Lys Ser Ser Ser Thr Ala Tyr 
65                  70                  75                  80  


Met Glu Leu Arg Ser Leu Thr Ser Glu Asp Ser Ala Val Tyr Tyr Cys 
                85                  90                  95      


Thr Arg Phe Tyr Ser Tyr Thr Tyr Trp Gly Gln Gly Thr Leu Val Thr 
            100                 105                 110         


Val Ser Ala Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly 
        115                 120                 125             


Gly Ser Asp Val Val Met Thr Gln Thr Pro Leu Ser Leu Pro Val Ser 
    130                 135                 140                 


Leu Gly Asp Gln Ala Ser Ile Ser Cys Arg Ser Ser Gln Ser Leu Val 
145                 150                 155                 160 


His Ser Asn Gly Asn Thr Tyr Leu His Trp Tyr Leu Gln Lys Pro Gly 
                165                 170                 175     


Gln Ser Pro Lys Leu Leu Ile Tyr Lys Val Ser Asn Arg Phe Ser Gly 
            180                 185                 190         


Val Pro Asp Arg Phe Ser Gly Ser Gly Ser Gly Thr Asp Phe Thr Leu 
        195                 200                 205             


Lys Ile Ser Arg Val Glu Ala Glu Asp Leu Gly Val Tyr Phe Cys Ser 
    210                 215                 220                 


Gln Asn Thr His Val Pro Pro Thr Phe Gly Ser Gly Thr Lys Leu Glu 
225                 230                 235                 240 


Ile Lys Glu Pro Lys Ser Cys Asp Lys Thr His Thr Cys Pro Pro Cys 
                245                 250                 255     


Pro Asp Pro Lys Phe Trp Val Leu Val Val Val Gly Gly Val Leu Ala 
            260                 265                 270         


Cys Tyr Ser Leu Leu Val Thr Val Ala Phe Ile Ile Phe Leu Asn Arg 
        275                 280                 285             


Arg Arg Arg Arg Glu Arg Arg Asp Leu Phe Thr Glu Ser Trp Asp Thr 
    290                 295                 300                 


Gln Lys Ala Pro Asn Asn Tyr Arg Ser Pro Ile Ser Thr Ser Gln Pro 
305                 310                 315                 320 


Thr Asn Gln Ser Met Asp Asp Thr Arg Glu Asp Ile Tyr Val Asn Tyr 
                325                 330                 335     


Pro Thr Phe Ser Arg Arg Pro Lys Thr Arg Val Lys Arg Gly Arg Lys 
            340                 345                 350         


Lys Leu Leu Tyr Ile Phe Lys Gln Pro Phe Met Arg Pro Val Gln Thr 
        355                 360                 365             


Thr Gln Glu Glu Asp Gly Cys Ser Cys Arg Phe Pro Glu Glu Glu Glu 
    370                 375                 380                 


Gly Gly Cys Glu Leu Arg Val Lys Phe Ser Arg Ser Ala Asp Ala Pro 
385                 390                 395                 400 


Ala Tyr Gln Gln Gly Gln Asn Gln Leu Tyr Asn Glu Leu Asn Leu Gly 
                405                 410                 415     


Arg Arg Glu Glu Tyr Asp Val Leu Asp Lys Arg Arg Gly Arg Asp Pro 
            420                 425                 430         


Glu Met Gly Gly Lys Pro Arg Arg Lys Asn Pro Gln Glu Gly Leu Tyr 
        435                 440                 445             


Asn Glu Leu Gln Lys Asp Lys Met Ala Glu Ala Tyr Ser Glu Ile Gly 
    450                 455                 460                 


Met Lys Gly Glu Arg Arg Arg Gly Lys Gly His Asp Gly Leu Tyr Gln 
465                 470                 475                 480 


Gly Leu Ser Thr Ala Thr Lys Asp Thr Tyr Asp Ala Leu His Met Gln 
                485                 490                 495     


Ala Leu Pro Pro Arg 
            500     


<210>  85
<211>  457
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  scFV GC33/hIgG1 hinge/CD28 TMD/ CD226 ICD v1/CD3zeta

<400>  85

Gln Val Gln Leu Gln Gln Ser Gly Ala Glu Leu Val Arg Pro Gly Ala 
1               5                   10                  15      


Ser Val Lys Leu Ser Cys Lys Ala Ser Gly Tyr Thr Phe Thr Asp Tyr 
            20                  25                  30          


Glu Met His Trp Val Lys Gln Thr Pro Val His Gly Leu Lys Trp Ile 
        35                  40                  45              


Gly Ala Leu Asp Pro Lys Thr Gly Asp Thr Ala Tyr Ser Gln Lys Phe 
    50                  55                  60                  


Lys Gly Lys Ala Thr Leu Thr Ala Asp Lys Ser Ser Ser Thr Ala Tyr 
65                  70                  75                  80  


Met Glu Leu Arg Ser Leu Thr Ser Glu Asp Ser Ala Val Tyr Tyr Cys 
                85                  90                  95      


Thr Arg Phe Tyr Ser Tyr Thr Tyr Trp Gly Gln Gly Thr Leu Val Thr 
            100                 105                 110         


Val Ser Ala Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly 
        115                 120                 125             


Gly Ser Asp Val Val Met Thr Gln Thr Pro Leu Ser Leu Pro Val Ser 
    130                 135                 140                 


Leu Gly Asp Gln Ala Ser Ile Ser Cys Arg Ser Ser Gln Ser Leu Val 
145                 150                 155                 160 


His Ser Asn Gly Asn Thr Tyr Leu His Trp Tyr Leu Gln Lys Pro Gly 
                165                 170                 175     


Gln Ser Pro Lys Leu Leu Ile Tyr Lys Val Ser Asn Arg Phe Ser Gly 
            180                 185                 190         


Val Pro Asp Arg Phe Ser Gly Ser Gly Ser Gly Thr Asp Phe Thr Leu 
        195                 200                 205             


Lys Ile Ser Arg Val Glu Ala Glu Asp Leu Gly Val Tyr Phe Cys Ser 
    210                 215                 220                 


Gln Asn Thr His Val Pro Pro Thr Phe Gly Ser Gly Thr Lys Leu Glu 
225                 230                 235                 240 


Ile Lys Glu Pro Lys Ser Cys Asp Lys Thr His Thr Cys Pro Pro Cys 
                245                 250                 255     


Pro Asp Pro Lys Phe Trp Val Leu Val Val Val Gly Gly Val Leu Ala 
            260                 265                 270         


Cys Tyr Ser Leu Leu Val Thr Val Ala Phe Ile Ile Asn Arg Arg Arg 
        275                 280                 285             


Arg Arg Glu Arg Arg Asp Leu Phe Thr Glu Ser Trp Asp Thr Gln Lys 
    290                 295                 300                 


Ala Pro Asn Asn Tyr Arg Ser Pro Ile Ser Thr Ser Gln Pro Thr Asn 
305                 310                 315                 320 


Gln Ser Met Asp Asp Thr Arg Glu Asp Ile Tyr Val Asn Tyr Pro Thr 
                325                 330                 335     


Phe Ser Arg Arg Pro Lys Thr Arg Val Arg Val Lys Phe Ser Arg Ser 
            340                 345                 350         


Ala Asp Ala Pro Ala Tyr Gln Gln Gly Gln Asn Gln Leu Tyr Asn Glu 
        355                 360                 365             


Leu Asn Leu Gly Arg Arg Glu Glu Tyr Asp Val Leu Asp Lys Arg Arg 
    370                 375                 380                 


Gly Arg Asp Pro Glu Met Gly Gly Lys Pro Arg Arg Lys Asn Pro Gln 
385                 390                 395                 400 


Glu Gly Leu Tyr Asn Glu Leu Gln Lys Asp Lys Met Ala Glu Ala Tyr 
                405                 410                 415     


Ser Glu Ile Gly Met Lys Gly Glu Arg Arg Arg Gly Lys Gly His Asp 
            420                 425                 430         


Gly Leu Tyr Gln Gly Leu Ser Thr Ala Thr Lys Asp Thr Tyr Asp Ala 
        435                 440                 445             


Leu His Met Gln Ala Leu Pro Pro Arg 
    450                 455         


<210>  86
<211>  459
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  scFV GC33/hIgG1 hinge/CD28 TMD/ CD226 ICD v2/CD3zeta

<400>  86

Gln Val Gln Leu Gln Gln Ser Gly Ala Glu Leu Val Arg Pro Gly Ala 
1               5                   10                  15      


Ser Val Lys Leu Ser Cys Lys Ala Ser Gly Tyr Thr Phe Thr Asp Tyr 
            20                  25                  30          


Glu Met His Trp Val Lys Gln Thr Pro Val His Gly Leu Lys Trp Ile 
        35                  40                  45              


Gly Ala Leu Asp Pro Lys Thr Gly Asp Thr Ala Tyr Ser Gln Lys Phe 
    50                  55                  60                  


Lys Gly Lys Ala Thr Leu Thr Ala Asp Lys Ser Ser Ser Thr Ala Tyr 
65                  70                  75                  80  


Met Glu Leu Arg Ser Leu Thr Ser Glu Asp Ser Ala Val Tyr Tyr Cys 
                85                  90                  95      


Thr Arg Phe Tyr Ser Tyr Thr Tyr Trp Gly Gln Gly Thr Leu Val Thr 
            100                 105                 110         


Val Ser Ala Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly 
        115                 120                 125             


Gly Ser Asp Val Val Met Thr Gln Thr Pro Leu Ser Leu Pro Val Ser 
    130                 135                 140                 


Leu Gly Asp Gln Ala Ser Ile Ser Cys Arg Ser Ser Gln Ser Leu Val 
145                 150                 155                 160 


His Ser Asn Gly Asn Thr Tyr Leu His Trp Tyr Leu Gln Lys Pro Gly 
                165                 170                 175     


Gln Ser Pro Lys Leu Leu Ile Tyr Lys Val Ser Asn Arg Phe Ser Gly 
            180                 185                 190         


Val Pro Asp Arg Phe Ser Gly Ser Gly Ser Gly Thr Asp Phe Thr Leu 
        195                 200                 205             


Lys Ile Ser Arg Val Glu Ala Glu Asp Leu Gly Val Tyr Phe Cys Ser 
    210                 215                 220                 


Gln Asn Thr His Val Pro Pro Thr Phe Gly Ser Gly Thr Lys Leu Glu 
225                 230                 235                 240 


Ile Lys Glu Pro Lys Ser Cys Asp Lys Thr His Thr Cys Pro Pro Cys 
                245                 250                 255     


Pro Asp Pro Lys Phe Trp Val Leu Val Val Val Gly Gly Val Leu Ala 
            260                 265                 270         


Cys Tyr Ser Leu Leu Val Thr Val Ala Phe Ile Ile Phe Leu Asn Arg 
        275                 280                 285             


Arg Arg Arg Arg Glu Arg Arg Asp Leu Phe Thr Glu Ser Trp Asp Thr 
    290                 295                 300                 


Gln Lys Ala Pro Asn Asn Tyr Arg Ser Pro Ile Ser Thr Ser Gln Pro 
305                 310                 315                 320 


Thr Asn Gln Ser Met Asp Asp Thr Arg Glu Asp Ile Tyr Val Asn Tyr 
                325                 330                 335     


Pro Thr Phe Ser Arg Arg Pro Lys Thr Arg Val Arg Val Lys Phe Ser 
            340                 345                 350         


Arg Ser Ala Asp Ala Pro Ala Tyr Gln Gln Gly Gln Asn Gln Leu Tyr 
        355                 360                 365             


Asn Glu Leu Asn Leu Gly Arg Arg Glu Glu Tyr Asp Val Leu Asp Lys 
    370                 375                 380                 


Arg Arg Gly Arg Asp Pro Glu Met Gly Gly Lys Pro Arg Arg Lys Asn 
385                 390                 395                 400 


Pro Gln Glu Gly Leu Tyr Asn Glu Leu Gln Lys Asp Lys Met Ala Glu 
                405                 410                 415     


Ala Tyr Ser Glu Ile Gly Met Lys Gly Glu Arg Arg Arg Gly Lys Gly 
            420                 425                 430         


His Asp Gly Leu Tyr Gln Gly Leu Ser Thr Ala Thr Lys Asp Thr Tyr 
        435                 440                 445             


Asp Ala Leu His Met Gln Ala Leu Pro Pro Arg 
    450                 455                 


<210>  87
<211>  440
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  scFV GC33/hIgG1 hinge/CD28 TMD/ CD28 ICD/CD3zeta

<400>  87

Gln Val Gln Leu Gln Gln Ser Gly Ala Glu Leu Val Arg Pro Gly Ala 
1               5                   10                  15      


Ser Val Lys Leu Ser Cys Lys Ala Ser Gly Tyr Thr Phe Thr Asp Tyr 
            20                  25                  30          


Glu Met His Trp Val Lys Gln Thr Pro Val His Gly Leu Lys Trp Ile 
        35                  40                  45              


Gly Ala Leu Asp Pro Lys Thr Gly Asp Thr Ala Tyr Ser Gln Lys Phe 
    50                  55                  60                  


Lys Gly Lys Ala Thr Leu Thr Ala Asp Lys Ser Ser Ser Thr Ala Tyr 
65                  70                  75                  80  


Met Glu Leu Arg Ser Leu Thr Ser Glu Asp Ser Ala Val Tyr Tyr Cys 
                85                  90                  95      


Thr Arg Phe Tyr Ser Tyr Thr Tyr Trp Gly Gln Gly Thr Leu Val Thr 
            100                 105                 110         


Val Ser Ala Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly 
        115                 120                 125             


Gly Ser Asp Val Val Met Thr Gln Thr Pro Leu Ser Leu Pro Val Ser 
    130                 135                 140                 


Leu Gly Asp Gln Ala Ser Ile Ser Cys Arg Ser Ser Gln Ser Leu Val 
145                 150                 155                 160 


His Ser Asn Gly Asn Thr Tyr Leu His Trp Tyr Leu Gln Lys Pro Gly 
                165                 170                 175     


Gln Ser Pro Lys Leu Leu Ile Tyr Lys Val Ser Asn Arg Phe Ser Gly 
            180                 185                 190         


Val Pro Asp Arg Phe Ser Gly Ser Gly Ser Gly Thr Asp Phe Thr Leu 
        195                 200                 205             


Lys Ile Ser Arg Val Glu Ala Glu Asp Leu Gly Val Tyr Phe Cys Ser 
    210                 215                 220                 


Gln Asn Thr His Val Pro Pro Thr Phe Gly Ser Gly Thr Lys Leu Glu 
225                 230                 235                 240 


Ile Lys Glu Pro Lys Ser Cys Asp Lys Thr His Thr Cys Pro Pro Cys 
                245                 250                 255     


Pro Asp Pro Lys Phe Trp Val Leu Val Val Val Gly Gly Val Leu Ala 
            260                 265                 270         


Cys Tyr Ser Leu Leu Val Thr Val Ala Phe Ile Ile Phe Trp Val Arg 
        275                 280                 285             


Ser Lys Arg Ser Arg Leu Leu His Ser Asp Tyr Met Asn Met Thr Pro 
    290                 295                 300                 


Arg Arg Pro Gly Pro Thr Arg Lys His Tyr Gln Pro Tyr Ala Pro Pro 
305                 310                 315                 320 


Arg Asp Phe Ala Ala Tyr Arg Ser Arg Val Lys Phe Ser Arg Ser Ala 
                325                 330                 335     


Asp Ala Pro Ala Tyr Gln Gln Gly Gln Asn Gln Leu Tyr Asn Glu Leu 
            340                 345                 350         


Asn Leu Gly Arg Arg Glu Glu Tyr Asp Val Leu Asp Lys Arg Arg Gly 
        355                 360                 365             


Arg Asp Pro Glu Met Gly Gly Lys Pro Arg Arg Lys Asn Pro Gln Glu 
    370                 375                 380                 


Gly Leu Tyr Asn Glu Leu Gln Lys Asp Lys Met Ala Glu Ala Tyr Ser 
385                 390                 395                 400 


Glu Ile Gly Met Lys Gly Glu Arg Arg Arg Gly Lys Gly His Asp Gly 
                405                 410                 415     


Leu Tyr Gln Gly Leu Ser Thr Ala Thr Lys Asp Thr Tyr Asp Ala Leu 
            420                 425                 430         


His Met Gln Ala Leu Pro Pro Arg 
        435                 440 


<210>  88
<211>  501
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  scFV GC33/hIgG1 hinge/CD28 TMD/ CD226 ICD v1/ CD28 ICD/CD3zeta

<400>  88

Gln Val Gln Leu Gln Gln Ser Gly Ala Glu Leu Val Arg Pro Gly Ala 
1               5                   10                  15      


Ser Val Lys Leu Ser Cys Lys Ala Ser Gly Tyr Thr Phe Thr Asp Tyr 
            20                  25                  30          


Glu Met His Trp Val Lys Gln Thr Pro Val His Gly Leu Lys Trp Ile 
        35                  40                  45              


Gly Ala Leu Asp Pro Lys Thr Gly Asp Thr Ala Tyr Ser Gln Lys Phe 
    50                  55                  60                  


Lys Gly Lys Ala Thr Leu Thr Ala Asp Lys Ser Ser Ser Thr Ala Tyr 
65                  70                  75                  80  


Met Glu Leu Arg Ser Leu Thr Ser Glu Asp Ser Ala Val Tyr Tyr Cys 
                85                  90                  95      


Thr Arg Phe Tyr Ser Tyr Thr Tyr Trp Gly Gln Gly Thr Leu Val Thr 
            100                 105                 110         


Val Ser Ala Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly 
        115                 120                 125             


Gly Ser Asp Val Val Met Thr Gln Thr Pro Leu Ser Leu Pro Val Ser 
    130                 135                 140                 


Leu Gly Asp Gln Ala Ser Ile Ser Cys Arg Ser Ser Gln Ser Leu Val 
145                 150                 155                 160 


His Ser Asn Gly Asn Thr Tyr Leu His Trp Tyr Leu Gln Lys Pro Gly 
                165                 170                 175     


Gln Ser Pro Lys Leu Leu Ile Tyr Lys Val Ser Asn Arg Phe Ser Gly 
            180                 185                 190         


Val Pro Asp Arg Phe Ser Gly Ser Gly Ser Gly Thr Asp Phe Thr Leu 
        195                 200                 205             


Lys Ile Ser Arg Val Glu Ala Glu Asp Leu Gly Val Tyr Phe Cys Ser 
    210                 215                 220                 


Gln Asn Thr His Val Pro Pro Thr Phe Gly Ser Gly Thr Lys Leu Glu 
225                 230                 235                 240 


Ile Lys Glu Pro Lys Ser Cys Asp Lys Thr His Thr Cys Pro Pro Cys 
                245                 250                 255     


Pro Asp Pro Lys Phe Trp Val Leu Val Val Val Gly Gly Val Leu Ala 
            260                 265                 270         


Cys Tyr Ser Leu Leu Val Thr Val Ala Phe Ile Ile Asn Arg Arg Arg 
        275                 280                 285             


Arg Arg Glu Arg Arg Asp Leu Phe Thr Glu Ser Trp Asp Thr Gln Lys 
    290                 295                 300                 


Ala Pro Asn Asn Tyr Arg Ser Pro Ile Ser Thr Ser Gln Pro Thr Asn 
305                 310                 315                 320 


Gln Ser Met Asp Asp Thr Arg Glu Asp Ile Tyr Val Asn Tyr Pro Thr 
                325                 330                 335     


Phe Ser Arg Arg Pro Lys Thr Arg Val Phe Trp Val Arg Ser Lys Arg 
            340                 345                 350         


Ser Arg Leu Leu His Ser Asp Tyr Met Asn Met Thr Pro Arg Arg Pro 
        355                 360                 365             


Gly Pro Thr Arg Lys His Tyr Gln Pro Tyr Ala Pro Pro Arg Asp Phe 
    370                 375                 380                 


Ala Ala Tyr Arg Ser Arg Val Lys Phe Ser Arg Ser Ala Asp Ala Pro 
385                 390                 395                 400 


Ala Tyr Gln Gln Gly Gln Asn Gln Leu Tyr Asn Glu Leu Asn Leu Gly 
                405                 410                 415     


Arg Arg Glu Glu Tyr Asp Val Leu Asp Lys Arg Arg Gly Arg Asp Pro 
            420                 425                 430         


Glu Met Gly Gly Lys Pro Arg Arg Lys Asn Pro Gln Glu Gly Leu Tyr 
        435                 440                 445             


Asn Glu Leu Gln Lys Asp Lys Met Ala Glu Ala Tyr Ser Glu Ile Gly 
    450                 455                 460                 


Met Lys Gly Glu Arg Arg Arg Gly Lys Gly His Asp Gly Leu Tyr Gln 
465                 470                 475                 480 


Gly Leu Ser Thr Ala Thr Lys Asp Thr Tyr Asp Ala Leu His Met Gln 
                485                 490                 495     


Ala Leu Pro Pro Arg 
            500     


<210>  89
<211>  503
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  scFV GC33/hIgG1 hinge/CD28 TMD/ CD226 ICD v2/ CD28 ICD/CD3zeta

<400>  89

Gln Val Gln Leu Gln Gln Ser Gly Ala Glu Leu Val Arg Pro Gly Ala 
1               5                   10                  15      


Ser Val Lys Leu Ser Cys Lys Ala Ser Gly Tyr Thr Phe Thr Asp Tyr 
            20                  25                  30          


Glu Met His Trp Val Lys Gln Thr Pro Val His Gly Leu Lys Trp Ile 
        35                  40                  45              


Gly Ala Leu Asp Pro Lys Thr Gly Asp Thr Ala Tyr Ser Gln Lys Phe 
    50                  55                  60                  


Lys Gly Lys Ala Thr Leu Thr Ala Asp Lys Ser Ser Ser Thr Ala Tyr 
65                  70                  75                  80  


Met Glu Leu Arg Ser Leu Thr Ser Glu Asp Ser Ala Val Tyr Tyr Cys 
                85                  90                  95      


Thr Arg Phe Tyr Ser Tyr Thr Tyr Trp Gly Gln Gly Thr Leu Val Thr 
            100                 105                 110         


Val Ser Ala Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly 
        115                 120                 125             


Gly Ser Asp Val Val Met Thr Gln Thr Pro Leu Ser Leu Pro Val Ser 
    130                 135                 140                 


Leu Gly Asp Gln Ala Ser Ile Ser Cys Arg Ser Ser Gln Ser Leu Val 
145                 150                 155                 160 


His Ser Asn Gly Asn Thr Tyr Leu His Trp Tyr Leu Gln Lys Pro Gly 
                165                 170                 175     


Gln Ser Pro Lys Leu Leu Ile Tyr Lys Val Ser Asn Arg Phe Ser Gly 
            180                 185                 190         


Val Pro Asp Arg Phe Ser Gly Ser Gly Ser Gly Thr Asp Phe Thr Leu 
        195                 200                 205             


Lys Ile Ser Arg Val Glu Ala Glu Asp Leu Gly Val Tyr Phe Cys Ser 
    210                 215                 220                 


Gln Asn Thr His Val Pro Pro Thr Phe Gly Ser Gly Thr Lys Leu Glu 
225                 230                 235                 240 


Ile Lys Glu Pro Lys Ser Cys Asp Lys Thr His Thr Cys Pro Pro Cys 
                245                 250                 255     


Pro Asp Pro Lys Phe Trp Val Leu Val Val Val Gly Gly Val Leu Ala 
            260                 265                 270         


Cys Tyr Ser Leu Leu Val Thr Val Ala Phe Ile Ile Phe Leu Asn Arg 
        275                 280                 285             


Arg Arg Arg Arg Glu Arg Arg Asp Leu Phe Thr Glu Ser Trp Asp Thr 
    290                 295                 300                 


Gln Lys Ala Pro Asn Asn Tyr Arg Ser Pro Ile Ser Thr Ser Gln Pro 
305                 310                 315                 320 


Thr Asn Gln Ser Met Asp Asp Thr Arg Glu Asp Ile Tyr Val Asn Tyr 
                325                 330                 335     


Pro Thr Phe Ser Arg Arg Pro Lys Thr Arg Val Phe Trp Val Arg Ser 
            340                 345                 350         


Lys Arg Ser Arg Leu Leu His Ser Asp Tyr Met Asn Met Thr Pro Arg 
        355                 360                 365             


Arg Pro Gly Pro Thr Arg Lys His Tyr Gln Pro Tyr Ala Pro Pro Arg 
    370                 375                 380                 


Asp Phe Ala Ala Tyr Arg Ser Arg Val Lys Phe Ser Arg Ser Ala Asp 
385                 390                 395                 400 


Ala Pro Ala Tyr Gln Gln Gly Gln Asn Gln Leu Tyr Asn Glu Leu Asn 
                405                 410                 415     


Leu Gly Arg Arg Glu Glu Tyr Asp Val Leu Asp Lys Arg Arg Gly Arg 
            420                 425                 430         


Asp Pro Glu Met Gly Gly Lys Pro Arg Arg Lys Asn Pro Gln Glu Gly 
        435                 440                 445             


Leu Tyr Asn Glu Leu Gln Lys Asp Lys Met Ala Glu Ala Tyr Ser Glu 
    450                 455                 460                 


Ile Gly Met Lys Gly Glu Arg Arg Arg Gly Lys Gly His Asp Gly Leu 
465                 470                 475                 480 


Tyr Gln Gly Leu Ser Thr Ala Thr Lys Asp Thr Tyr Asp Ala Leu His 
                485                 490                 495     


Met Gln Ala Leu Pro Pro Arg 
            500             


<210>  90
<211>  501
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  scFV GC33/hIgG1 hinge/CD28 TMD/ CD28 ICD/CD226 ICD v1/CD3zeta

<400>  90

Gln Val Gln Leu Gln Gln Ser Gly Ala Glu Leu Val Arg Pro Gly Ala 
1               5                   10                  15      


Ser Val Lys Leu Ser Cys Lys Ala Ser Gly Tyr Thr Phe Thr Asp Tyr 
            20                  25                  30          


Glu Met His Trp Val Lys Gln Thr Pro Val His Gly Leu Lys Trp Ile 
        35                  40                  45              


Gly Ala Leu Asp Pro Lys Thr Gly Asp Thr Ala Tyr Ser Gln Lys Phe 
    50                  55                  60                  


Lys Gly Lys Ala Thr Leu Thr Ala Asp Lys Ser Ser Ser Thr Ala Tyr 
65                  70                  75                  80  


Met Glu Leu Arg Ser Leu Thr Ser Glu Asp Ser Ala Val Tyr Tyr Cys 
                85                  90                  95      


Thr Arg Phe Tyr Ser Tyr Thr Tyr Trp Gly Gln Gly Thr Leu Val Thr 
            100                 105                 110         


Val Ser Ala Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly 
        115                 120                 125             


Gly Ser Asp Val Val Met Thr Gln Thr Pro Leu Ser Leu Pro Val Ser 
    130                 135                 140                 


Leu Gly Asp Gln Ala Ser Ile Ser Cys Arg Ser Ser Gln Ser Leu Val 
145                 150                 155                 160 


His Ser Asn Gly Asn Thr Tyr Leu His Trp Tyr Leu Gln Lys Pro Gly 
                165                 170                 175     


Gln Ser Pro Lys Leu Leu Ile Tyr Lys Val Ser Asn Arg Phe Ser Gly 
            180                 185                 190         


Val Pro Asp Arg Phe Ser Gly Ser Gly Ser Gly Thr Asp Phe Thr Leu 
        195                 200                 205             


Lys Ile Ser Arg Val Glu Ala Glu Asp Leu Gly Val Tyr Phe Cys Ser 
    210                 215                 220                 


Gln Asn Thr His Val Pro Pro Thr Phe Gly Ser Gly Thr Lys Leu Glu 
225                 230                 235                 240 


Ile Lys Glu Pro Lys Ser Cys Asp Lys Thr His Thr Cys Pro Pro Cys 
                245                 250                 255     


Pro Asp Pro Lys Phe Trp Val Leu Val Val Val Gly Gly Val Leu Ala 
            260                 265                 270         


Cys Tyr Ser Leu Leu Val Thr Val Ala Phe Ile Ile Phe Trp Val Arg 
        275                 280                 285             


Ser Lys Arg Ser Arg Leu Leu His Ser Asp Tyr Met Asn Met Thr Pro 
    290                 295                 300                 


Arg Arg Pro Gly Pro Thr Arg Lys His Tyr Gln Pro Tyr Ala Pro Pro 
305                 310                 315                 320 


Arg Asp Phe Ala Ala Tyr Arg Ser Asn Arg Arg Arg Arg Arg Glu Arg 
                325                 330                 335     


Arg Asp Leu Phe Thr Glu Ser Trp Asp Thr Gln Lys Ala Pro Asn Asn 
            340                 345                 350         


Tyr Arg Ser Pro Ile Ser Thr Ser Gln Pro Thr Asn Gln Ser Met Asp 
        355                 360                 365             


Asp Thr Arg Glu Asp Ile Tyr Val Asn Tyr Pro Thr Phe Ser Arg Arg 
    370                 375                 380                 


Pro Lys Thr Arg Val Arg Val Lys Phe Ser Arg Ser Ala Asp Ala Pro 
385                 390                 395                 400 


Ala Tyr Gln Gln Gly Gln Asn Gln Leu Tyr Asn Glu Leu Asn Leu Gly 
                405                 410                 415     


Arg Arg Glu Glu Tyr Asp Val Leu Asp Lys Arg Arg Gly Arg Asp Pro 
            420                 425                 430         


Glu Met Gly Gly Lys Pro Arg Arg Lys Asn Pro Gln Glu Gly Leu Tyr 
        435                 440                 445             


Asn Glu Leu Gln Lys Asp Lys Met Ala Glu Ala Tyr Ser Glu Ile Gly 
    450                 455                 460                 


Met Lys Gly Glu Arg Arg Arg Gly Lys Gly His Asp Gly Leu Tyr Gln 
465                 470                 475                 480 


Gly Leu Ser Thr Ala Thr Lys Asp Thr Tyr Asp Ala Leu His Met Gln 
                485                 490                 495     


Ala Leu Pro Pro Arg 
            500     


<210>  91
<211>  482
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  scFV GC33/hIgG1 hinge/CD28 TMD/ CD28 ICD/41BB/CD3zeta

<400>  91

Gln Val Gln Leu Gln Gln Ser Gly Ala Glu Leu Val Arg Pro Gly Ala 
1               5                   10                  15      


Ser Val Lys Leu Ser Cys Lys Ala Ser Gly Tyr Thr Phe Thr Asp Tyr 
            20                  25                  30          


Glu Met His Trp Val Lys Gln Thr Pro Val His Gly Leu Lys Trp Ile 
        35                  40                  45              


Gly Ala Leu Asp Pro Lys Thr Gly Asp Thr Ala Tyr Ser Gln Lys Phe 
    50                  55                  60                  


Lys Gly Lys Ala Thr Leu Thr Ala Asp Lys Ser Ser Ser Thr Ala Tyr 
65                  70                  75                  80  


Met Glu Leu Arg Ser Leu Thr Ser Glu Asp Ser Ala Val Tyr Tyr Cys 
                85                  90                  95      


Thr Arg Phe Tyr Ser Tyr Thr Tyr Trp Gly Gln Gly Thr Leu Val Thr 
            100                 105                 110         


Val Ser Ala Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly 
        115                 120                 125             


Gly Ser Asp Val Val Met Thr Gln Thr Pro Leu Ser Leu Pro Val Ser 
    130                 135                 140                 


Leu Gly Asp Gln Ala Ser Ile Ser Cys Arg Ser Ser Gln Ser Leu Val 
145                 150                 155                 160 


His Ser Asn Gly Asn Thr Tyr Leu His Trp Tyr Leu Gln Lys Pro Gly 
                165                 170                 175     


Gln Ser Pro Lys Leu Leu Ile Tyr Lys Val Ser Asn Arg Phe Ser Gly 
            180                 185                 190         


Val Pro Asp Arg Phe Ser Gly Ser Gly Ser Gly Thr Asp Phe Thr Leu 
        195                 200                 205             


Lys Ile Ser Arg Val Glu Ala Glu Asp Leu Gly Val Tyr Phe Cys Ser 
    210                 215                 220                 


Gln Asn Thr His Val Pro Pro Thr Phe Gly Ser Gly Thr Lys Leu Glu 
225                 230                 235                 240 


Ile Lys Glu Pro Lys Ser Cys Asp Lys Thr His Thr Cys Pro Pro Cys 
                245                 250                 255     


Pro Asp Pro Lys Phe Trp Val Leu Val Val Val Gly Gly Val Leu Ala 
            260                 265                 270         


Cys Tyr Ser Leu Leu Val Thr Val Ala Phe Ile Ile Phe Trp Val Arg 
        275                 280                 285             


Ser Lys Arg Ser Arg Leu Leu His Ser Asp Tyr Met Asn Met Thr Pro 
    290                 295                 300                 


Arg Arg Pro Gly Pro Thr Arg Lys His Tyr Gln Pro Tyr Ala Pro Pro 
305                 310                 315                 320 


Arg Asp Phe Ala Ala Tyr Arg Ser Lys Arg Gly Arg Lys Lys Leu Leu 
                325                 330                 335     


Tyr Ile Phe Lys Gln Pro Phe Met Arg Pro Val Gln Thr Thr Gln Glu 
            340                 345                 350         


Glu Asp Gly Cys Ser Cys Arg Phe Pro Glu Glu Glu Glu Gly Gly Cys 
        355                 360                 365             


Glu Leu Arg Val Lys Phe Ser Arg Ser Ala Asp Ala Pro Ala Tyr Gln 
    370                 375                 380                 


Gln Gly Gln Asn Gln Leu Tyr Asn Glu Leu Asn Leu Gly Arg Arg Glu 
385                 390                 395                 400 


Glu Tyr Asp Val Leu Asp Lys Arg Arg Gly Arg Asp Pro Glu Met Gly 
                405                 410                 415     


Gly Lys Pro Arg Arg Lys Asn Pro Gln Glu Gly Leu Tyr Asn Glu Leu 
            420                 425                 430         


Gln Lys Asp Lys Met Ala Glu Ala Tyr Ser Glu Ile Gly Met Lys Gly 
        435                 440                 445             


Glu Arg Arg Arg Gly Lys Gly His Asp Gly Leu Tyr Gln Gly Leu Ser 
    450                 455                 460                 


Thr Ala Thr Lys Asp Thr Tyr Asp Ala Leu His Met Gln Ala Leu Pro 
465                 470                 475                 480 


Pro Arg 
        


<210>  92
<211>  543
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  scFV GC33/hIgG1 hinge/CD28 TMD/ CD226 ICD v1/ CD28 
       ICD/41BB/CD3zeta

<400>  92

Gln Val Gln Leu Gln Gln Ser Gly Ala Glu Leu Val Arg Pro Gly Ala 
1               5                   10                  15      


Ser Val Lys Leu Ser Cys Lys Ala Ser Gly Tyr Thr Phe Thr Asp Tyr 
            20                  25                  30          


Glu Met His Trp Val Lys Gln Thr Pro Val His Gly Leu Lys Trp Ile 
        35                  40                  45              


Gly Ala Leu Asp Pro Lys Thr Gly Asp Thr Ala Tyr Ser Gln Lys Phe 
    50                  55                  60                  


Lys Gly Lys Ala Thr Leu Thr Ala Asp Lys Ser Ser Ser Thr Ala Tyr 
65                  70                  75                  80  


Met Glu Leu Arg Ser Leu Thr Ser Glu Asp Ser Ala Val Tyr Tyr Cys 
                85                  90                  95      


Thr Arg Phe Tyr Ser Tyr Thr Tyr Trp Gly Gln Gly Thr Leu Val Thr 
            100                 105                 110         


Val Ser Ala Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly 
        115                 120                 125             


Gly Ser Asp Val Val Met Thr Gln Thr Pro Leu Ser Leu Pro Val Ser 
    130                 135                 140                 


Leu Gly Asp Gln Ala Ser Ile Ser Cys Arg Ser Ser Gln Ser Leu Val 
145                 150                 155                 160 


His Ser Asn Gly Asn Thr Tyr Leu His Trp Tyr Leu Gln Lys Pro Gly 
                165                 170                 175     


Gln Ser Pro Lys Leu Leu Ile Tyr Lys Val Ser Asn Arg Phe Ser Gly 
            180                 185                 190         


Val Pro Asp Arg Phe Ser Gly Ser Gly Ser Gly Thr Asp Phe Thr Leu 
        195                 200                 205             


Lys Ile Ser Arg Val Glu Ala Glu Asp Leu Gly Val Tyr Phe Cys Ser 
    210                 215                 220                 


Gln Asn Thr His Val Pro Pro Thr Phe Gly Ser Gly Thr Lys Leu Glu 
225                 230                 235                 240 


Ile Lys Glu Pro Lys Ser Cys Asp Lys Thr His Thr Cys Pro Pro Cys 
                245                 250                 255     


Pro Asp Pro Lys Phe Trp Val Leu Val Val Val Gly Gly Val Leu Ala 
            260                 265                 270         


Cys Tyr Ser Leu Leu Val Thr Val Ala Phe Ile Ile Asn Arg Arg Arg 
        275                 280                 285             


Arg Arg Glu Arg Arg Asp Leu Phe Thr Glu Ser Trp Asp Thr Gln Lys 
    290                 295                 300                 


Ala Pro Asn Asn Tyr Arg Ser Pro Ile Ser Thr Ser Gln Pro Thr Asn 
305                 310                 315                 320 


Gln Ser Met Asp Asp Thr Arg Glu Asp Ile Tyr Val Asn Tyr Pro Thr 
                325                 330                 335     


Phe Ser Arg Arg Pro Lys Thr Arg Val Phe Trp Val Arg Ser Lys Arg 
            340                 345                 350         


Ser Arg Leu Leu His Ser Asp Tyr Met Asn Met Thr Pro Arg Arg Pro 
        355                 360                 365             


Gly Pro Thr Arg Lys His Tyr Gln Pro Tyr Ala Pro Pro Arg Asp Phe 
    370                 375                 380                 


Ala Ala Tyr Arg Ser Lys Arg Gly Arg Lys Lys Leu Leu Tyr Ile Phe 
385                 390                 395                 400 


Lys Gln Pro Phe Met Arg Pro Val Gln Thr Thr Gln Glu Glu Asp Gly 
                405                 410                 415     


Cys Ser Cys Arg Phe Pro Glu Glu Glu Glu Gly Gly Cys Glu Leu Arg 
            420                 425                 430         


Val Lys Phe Ser Arg Ser Ala Asp Ala Pro Ala Tyr Gln Gln Gly Gln 
        435                 440                 445             


Asn Gln Leu Tyr Asn Glu Leu Asn Leu Gly Arg Arg Glu Glu Tyr Asp 
    450                 455                 460                 


Val Leu Asp Lys Arg Arg Gly Arg Asp Pro Glu Met Gly Gly Lys Pro 
465                 470                 475                 480 


Arg Arg Lys Asn Pro Gln Glu Gly Leu Tyr Asn Glu Leu Gln Lys Asp 
                485                 490                 495     


Lys Met Ala Glu Ala Tyr Ser Glu Ile Gly Met Lys Gly Glu Arg Arg 
            500                 505                 510         


Arg Gly Lys Gly His Asp Gly Leu Tyr Gln Gly Leu Ser Thr Ala Thr 
        515                 520                 525             


Lys Asp Thr Tyr Asp Ala Leu His Met Gln Ala Leu Pro Pro Arg 
    530                 535                 540             


<210>  93
<211>  545
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  scFV GC33/hIgG1 hinge/CD28 TMD/ CD226 ICD v2/ CD28 
       ICD/41BB/CD3zeta

<400>  93

Gln Val Gln Leu Gln Gln Ser Gly Ala Glu Leu Val Arg Pro Gly Ala 
1               5                   10                  15      


Ser Val Lys Leu Ser Cys Lys Ala Ser Gly Tyr Thr Phe Thr Asp Tyr 
            20                  25                  30          


Glu Met His Trp Val Lys Gln Thr Pro Val His Gly Leu Lys Trp Ile 
        35                  40                  45              


Gly Ala Leu Asp Pro Lys Thr Gly Asp Thr Ala Tyr Ser Gln Lys Phe 
    50                  55                  60                  


Lys Gly Lys Ala Thr Leu Thr Ala Asp Lys Ser Ser Ser Thr Ala Tyr 
65                  70                  75                  80  


Met Glu Leu Arg Ser Leu Thr Ser Glu Asp Ser Ala Val Tyr Tyr Cys 
                85                  90                  95      


Thr Arg Phe Tyr Ser Tyr Thr Tyr Trp Gly Gln Gly Thr Leu Val Thr 
            100                 105                 110         


Val Ser Ala Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly 
        115                 120                 125             


Gly Ser Asp Val Val Met Thr Gln Thr Pro Leu Ser Leu Pro Val Ser 
    130                 135                 140                 


Leu Gly Asp Gln Ala Ser Ile Ser Cys Arg Ser Ser Gln Ser Leu Val 
145                 150                 155                 160 


His Ser Asn Gly Asn Thr Tyr Leu His Trp Tyr Leu Gln Lys Pro Gly 
                165                 170                 175     


Gln Ser Pro Lys Leu Leu Ile Tyr Lys Val Ser Asn Arg Phe Ser Gly 
            180                 185                 190         


Val Pro Asp Arg Phe Ser Gly Ser Gly Ser Gly Thr Asp Phe Thr Leu 
        195                 200                 205             


Lys Ile Ser Arg Val Glu Ala Glu Asp Leu Gly Val Tyr Phe Cys Ser 
    210                 215                 220                 


Gln Asn Thr His Val Pro Pro Thr Phe Gly Ser Gly Thr Lys Leu Glu 
225                 230                 235                 240 


Ile Lys Glu Pro Lys Ser Cys Asp Lys Thr His Thr Cys Pro Pro Cys 
                245                 250                 255     


Pro Asp Pro Lys Phe Trp Val Leu Val Val Val Gly Gly Val Leu Ala 
            260                 265                 270         


Cys Tyr Ser Leu Leu Val Thr Val Ala Phe Ile Ile Phe Leu Asn Arg 
        275                 280                 285             


Arg Arg Arg Arg Glu Arg Arg Asp Leu Phe Thr Glu Ser Trp Asp Thr 
    290                 295                 300                 


Gln Lys Ala Pro Asn Asn Tyr Arg Ser Pro Ile Ser Thr Ser Gln Pro 
305                 310                 315                 320 


Thr Asn Gln Ser Met Asp Asp Thr Arg Glu Asp Ile Tyr Val Asn Tyr 
                325                 330                 335     


Pro Thr Phe Ser Arg Arg Pro Lys Thr Arg Val Phe Trp Val Arg Ser 
            340                 345                 350         


Lys Arg Ser Arg Leu Leu His Ser Asp Tyr Met Asn Met Thr Pro Arg 
        355                 360                 365             


Arg Pro Gly Pro Thr Arg Lys His Tyr Gln Pro Tyr Ala Pro Pro Arg 
    370                 375                 380                 


Asp Phe Ala Ala Tyr Arg Ser Lys Arg Gly Arg Lys Lys Leu Leu Tyr 
385                 390                 395                 400 


Ile Phe Lys Gln Pro Phe Met Arg Pro Val Gln Thr Thr Gln Glu Glu 
                405                 410                 415     


Asp Gly Cys Ser Cys Arg Phe Pro Glu Glu Glu Glu Gly Gly Cys Glu 
            420                 425                 430         


Leu Arg Val Lys Phe Ser Arg Ser Ala Asp Ala Pro Ala Tyr Gln Gln 
        435                 440                 445             


Gly Gln Asn Gln Leu Tyr Asn Glu Leu Asn Leu Gly Arg Arg Glu Glu 
    450                 455                 460                 


Tyr Asp Val Leu Asp Lys Arg Arg Gly Arg Asp Pro Glu Met Gly Gly 
465                 470                 475                 480 


Lys Pro Arg Arg Lys Asn Pro Gln Glu Gly Leu Tyr Asn Glu Leu Gln 
                485                 490                 495     


Lys Asp Lys Met Ala Glu Ala Tyr Ser Glu Ile Gly Met Lys Gly Glu 
            500                 505                 510         


Arg Arg Arg Gly Lys Gly His Asp Gly Leu Tyr Gln Gly Leu Ser Thr 
        515                 520                 525             


Ala Thr Lys Asp Thr Tyr Asp Ala Leu His Met Gln Ala Leu Pro Pro 
    530                 535                 540                 


Arg 
545 


<210>  94
<211>  543
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  scFV GC33/hIgG1 hinge/CD28 TMD/ CD28 ICD/CD226 ICD 
       v1/41BB/CD3zeta

<400>  94

Gln Val Gln Leu Gln Gln Ser Gly Ala Glu Leu Val Arg Pro Gly Ala 
1               5                   10                  15      


Ser Val Lys Leu Ser Cys Lys Ala Ser Gly Tyr Thr Phe Thr Asp Tyr 
            20                  25                  30          


Glu Met His Trp Val Lys Gln Thr Pro Val His Gly Leu Lys Trp Ile 
        35                  40                  45              


Gly Ala Leu Asp Pro Lys Thr Gly Asp Thr Ala Tyr Ser Gln Lys Phe 
    50                  55                  60                  


Lys Gly Lys Ala Thr Leu Thr Ala Asp Lys Ser Ser Ser Thr Ala Tyr 
65                  70                  75                  80  


Met Glu Leu Arg Ser Leu Thr Ser Glu Asp Ser Ala Val Tyr Tyr Cys 
                85                  90                  95      


Thr Arg Phe Tyr Ser Tyr Thr Tyr Trp Gly Gln Gly Thr Leu Val Thr 
            100                 105                 110         


Val Ser Ala Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly 
        115                 120                 125             


Gly Ser Asp Val Val Met Thr Gln Thr Pro Leu Ser Leu Pro Val Ser 
    130                 135                 140                 


Leu Gly Asp Gln Ala Ser Ile Ser Cys Arg Ser Ser Gln Ser Leu Val 
145                 150                 155                 160 


His Ser Asn Gly Asn Thr Tyr Leu His Trp Tyr Leu Gln Lys Pro Gly 
                165                 170                 175     


Gln Ser Pro Lys Leu Leu Ile Tyr Lys Val Ser Asn Arg Phe Ser Gly 
            180                 185                 190         


Val Pro Asp Arg Phe Ser Gly Ser Gly Ser Gly Thr Asp Phe Thr Leu 
        195                 200                 205             


Lys Ile Ser Arg Val Glu Ala Glu Asp Leu Gly Val Tyr Phe Cys Ser 
    210                 215                 220                 


Gln Asn Thr His Val Pro Pro Thr Phe Gly Ser Gly Thr Lys Leu Glu 
225                 230                 235                 240 


Ile Lys Glu Pro Lys Ser Cys Asp Lys Thr His Thr Cys Pro Pro Cys 
                245                 250                 255     


Pro Asp Pro Lys Phe Trp Val Leu Val Val Val Gly Gly Val Leu Ala 
            260                 265                 270         


Cys Tyr Ser Leu Leu Val Thr Val Ala Phe Ile Ile Phe Trp Val Arg 
        275                 280                 285             


Ser Lys Arg Ser Arg Leu Leu His Ser Asp Tyr Met Asn Met Thr Pro 
    290                 295                 300                 


Arg Arg Pro Gly Pro Thr Arg Lys His Tyr Gln Pro Tyr Ala Pro Pro 
305                 310                 315                 320 


Arg Asp Phe Ala Ala Tyr Arg Ser Asn Arg Arg Arg Arg Arg Glu Arg 
                325                 330                 335     


Arg Asp Leu Phe Thr Glu Ser Trp Asp Thr Gln Lys Ala Pro Asn Asn 
            340                 345                 350         


Tyr Arg Ser Pro Ile Ser Thr Ser Gln Pro Thr Asn Gln Ser Met Asp 
        355                 360                 365             


Asp Thr Arg Glu Asp Ile Tyr Val Asn Tyr Pro Thr Phe Ser Arg Arg 
    370                 375                 380                 


Pro Lys Thr Arg Val Lys Arg Gly Arg Lys Lys Leu Leu Tyr Ile Phe 
385                 390                 395                 400 


Lys Gln Pro Phe Met Arg Pro Val Gln Thr Thr Gln Glu Glu Asp Gly 
                405                 410                 415     


Cys Ser Cys Arg Phe Pro Glu Glu Glu Glu Gly Gly Cys Glu Leu Arg 
            420                 425                 430         


Val Lys Phe Ser Arg Ser Ala Asp Ala Pro Ala Tyr Gln Gln Gly Gln 
        435                 440                 445             


Asn Gln Leu Tyr Asn Glu Leu Asn Leu Gly Arg Arg Glu Glu Tyr Asp 
    450                 455                 460                 


Val Leu Asp Lys Arg Arg Gly Arg Asp Pro Glu Met Gly Gly Lys Pro 
465                 470                 475                 480 


Arg Arg Lys Asn Pro Gln Glu Gly Leu Tyr Asn Glu Leu Gln Lys Asp 
                485                 490                 495     


Lys Met Ala Glu Ala Tyr Ser Glu Ile Gly Met Lys Gly Glu Arg Arg 
            500                 505                 510         


Arg Gly Lys Gly His Asp Gly Leu Tyr Gln Gly Leu Ser Thr Ala Thr 
        515                 520                 525             


Lys Asp Thr Tyr Asp Ala Leu His Met Gln Ala Leu Pro Pro Arg 
    530                 535                 540             


<210>  95
<211>  496
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  scFV GC33/hIgG1 hinge/CD226 TMD/CD226 ICD v1/41BB/CD3zeta

<400>  95

Gln Val Gln Leu Gln Gln Ser Gly Ala Glu Leu Val Arg Pro Gly Ala 
1               5                   10                  15      


Ser Val Lys Leu Ser Cys Lys Ala Ser Gly Tyr Thr Phe Thr Asp Tyr 
            20                  25                  30          


Glu Met His Trp Val Lys Gln Thr Pro Val His Gly Leu Lys Trp Ile 
        35                  40                  45              


Gly Ala Leu Asp Pro Lys Thr Gly Asp Thr Ala Tyr Ser Gln Lys Phe 
    50                  55                  60                  


Lys Gly Lys Ala Thr Leu Thr Ala Asp Lys Ser Ser Ser Thr Ala Tyr 
65                  70                  75                  80  


Met Glu Leu Arg Ser Leu Thr Ser Glu Asp Ser Ala Val Tyr Tyr Cys 
                85                  90                  95      


Thr Arg Phe Tyr Ser Tyr Thr Tyr Trp Gly Gln Gly Thr Leu Val Thr 
            100                 105                 110         


Val Ser Ala Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly 
        115                 120                 125             


Gly Ser Asp Val Val Met Thr Gln Thr Pro Leu Ser Leu Pro Val Ser 
    130                 135                 140                 


Leu Gly Asp Gln Ala Ser Ile Ser Cys Arg Ser Ser Gln Ser Leu Val 
145                 150                 155                 160 


His Ser Asn Gly Asn Thr Tyr Leu His Trp Tyr Leu Gln Lys Pro Gly 
                165                 170                 175     


Gln Ser Pro Lys Leu Leu Ile Tyr Lys Val Ser Asn Arg Phe Ser Gly 
            180                 185                 190         


Val Pro Asp Arg Phe Ser Gly Ser Gly Ser Gly Thr Asp Phe Thr Leu 
        195                 200                 205             


Lys Ile Ser Arg Val Glu Ala Glu Asp Leu Gly Val Tyr Phe Cys Ser 
    210                 215                 220                 


Gln Asn Thr His Val Pro Pro Thr Phe Gly Ser Gly Thr Lys Leu Glu 
225                 230                 235                 240 


Ile Lys Glu Pro Lys Ser Cys Asp Lys Thr His Thr Cys Pro Pro Cys 
                245                 250                 255     


Pro Asp Pro Lys Gly Gly Thr Val Leu Leu Leu Leu Phe Val Ile Ser 
            260                 265                 270         


Ile Thr Thr Ile Ile Val Ile Phe Leu Asn Arg Arg Arg Arg Arg Glu 
        275                 280                 285             


Arg Arg Asp Leu Phe Thr Glu Ser Trp Asp Thr Gln Lys Ala Pro Asn 
    290                 295                 300                 


Asn Tyr Arg Ser Pro Ile Ser Thr Ser Gln Pro Thr Asn Gln Ser Met 
305                 310                 315                 320 


Asp Asp Thr Arg Glu Asp Ile Tyr Val Asn Tyr Pro Thr Phe Ser Arg 
                325                 330                 335     


Arg Pro Lys Thr Arg Val Lys Arg Gly Arg Lys Lys Leu Leu Tyr Ile 
            340                 345                 350         


Phe Lys Gln Pro Phe Met Arg Pro Val Gln Thr Thr Gln Glu Glu Asp 
        355                 360                 365             


Gly Cys Ser Cys Arg Phe Pro Glu Glu Glu Glu Gly Gly Cys Glu Leu 
    370                 375                 380                 


Arg Val Lys Phe Ser Arg Ser Ala Asp Ala Pro Ala Tyr Gln Gln Gly 
385                 390                 395                 400 


Gln Asn Gln Leu Tyr Asn Glu Leu Asn Leu Gly Arg Arg Glu Glu Tyr 
                405                 410                 415     


Asp Val Leu Asp Lys Arg Arg Gly Arg Asp Pro Glu Met Gly Gly Lys 
            420                 425                 430         


Pro Arg Arg Lys Asn Pro Gln Glu Gly Leu Tyr Asn Glu Leu Gln Lys 
        435                 440                 445             


Asp Lys Met Ala Glu Ala Tyr Ser Glu Ile Gly Met Lys Gly Glu Arg 
    450                 455                 460                 


Arg Arg Gly Lys Gly His Asp Gly Leu Tyr Gln Gly Leu Ser Thr Ala 
465                 470                 475                 480 


Thr Lys Asp Thr Tyr Asp Ala Leu His Met Gln Ala Leu Pro Pro Arg 
                485                 490                 495     


<210>  96
<211>  345
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  scFV 3-17I/hIgG1 hinge/CD226 TMD/CD226 ICD v1

<400>  96

Gln Val Gln Leu Val Gln Ser Gly Ala Glu Val Lys Lys Pro Gly Ser 
1               5                   10                  15      


Ser Val Lys Val Ser Cys Lys Ala Ser Gly Gly Thr Phe Ser Ser Tyr 
            20                  25                  30          


Ala Ile Ser Trp Val Arg Gln Ala Pro Gly Gln Gly Leu Glu Trp Met 
        35                  40                  45              


Gly Gly Ile Ile Pro Ile Phe Gly Thr Ala Asn Tyr Ala Gln Lys Phe 
    50                  55                  60                  


Gln Gly Arg Val Thr Ile Thr Ala Asp Glu Ser Thr Ser Thr Ala Tyr 
65                  70                  75                  80  


Met Glu Leu Ser Ser Leu Arg Ser Glu Asp Thr Ala Val Tyr Tyr Cys 
                85                  90                  95      


Ala Arg Gly Leu Leu Trp Asn Tyr Trp Gly Gln Gly Thr Leu Val Thr 
            100                 105                 110         


Val Ser Ser Lys Leu Ser Gly Ser Ala Ser Ala Pro Lys Leu Glu Glu 
        115                 120                 125             


Gly Glu Phe Ser Glu Ala Arg Val Glu Ile Val Met Thr Gln Ser Pro 
    130                 135                 140                 


Ala Thr Leu Ser Val Ser Pro Gly Glu Arg Ala Thr Leu Ser Cys Arg 
145                 150                 155                 160 


Ala Ser Gln Ser Val Ser Ser Asn Leu Ala Trp Tyr Gln Gln Lys Pro 
                165                 170                 175     


Gly Gln Ala Pro Arg Leu Ile Ile Tyr Gly Ala Ser Thr Thr Ala Ser 
            180                 185                 190         


Gly Ile Pro Ala Arg Phe Ser Ala Ser Gly Ser Gly Thr Asp Phe Thr 
        195                 200                 205             


Leu Thr Ile Ser Ser Leu Gln Ser Glu Asp Phe Ala Val Tyr Tyr Cys 
    210                 215                 220                 


Gln Gln Tyr Asn Asn Trp Pro Pro Ala Tyr Thr Phe Gly Gln Gly Thr 
225                 230                 235                 240 


Lys Leu Glu Ile Lys Glu Pro Lys Ser Cys Asp Lys Thr His Thr Cys 
                245                 250                 255     


Pro Pro Cys Pro Asp Pro Lys Gly Gly Thr Val Leu Leu Leu Leu Phe 
            260                 265                 270         


Val Ile Ser Ile Thr Thr Ile Ile Val Ile Phe Leu Asn Arg Arg Arg 
        275                 280                 285             


Arg Arg Glu Arg Arg Asp Leu Phe Thr Glu Ser Trp Asp Thr Gln Lys 
    290                 295                 300                 


Ala Pro Asn Asn Tyr Arg Ser Pro Ile Ser Thr Ser Gln Pro Thr Asn 
305                 310                 315                 320 


Gln Ser Met Asp Asp Thr Arg Glu Asp Ile Tyr Val Asn Tyr Pro Thr 
                325                 330                 335     


Phe Ser Arg Arg Pro Lys Thr Arg Val 
            340                 345 


<210>  97
<211>  396
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  scFV 3-17I/hIgG1 hinge/CD226 TMD/ CD3zeta

<400>  97

Gln Val Gln Leu Val Gln Ser Gly Ala Glu Val Lys Lys Pro Gly Ser 
1               5                   10                  15      


Ser Val Lys Val Ser Cys Lys Ala Ser Gly Gly Thr Phe Ser Ser Tyr 
            20                  25                  30          


Ala Ile Ser Trp Val Arg Gln Ala Pro Gly Gln Gly Leu Glu Trp Met 
        35                  40                  45              


Gly Gly Ile Ile Pro Ile Phe Gly Thr Ala Asn Tyr Ala Gln Lys Phe 
    50                  55                  60                  


Gln Gly Arg Val Thr Ile Thr Ala Asp Glu Ser Thr Ser Thr Ala Tyr 
65                  70                  75                  80  


Met Glu Leu Ser Ser Leu Arg Ser Glu Asp Thr Ala Val Tyr Tyr Cys 
                85                  90                  95      


Ala Arg Gly Leu Leu Trp Asn Tyr Trp Gly Gln Gly Thr Leu Val Thr 
            100                 105                 110         


Val Ser Ser Lys Leu Ser Gly Ser Ala Ser Ala Pro Lys Leu Glu Glu 
        115                 120                 125             


Gly Glu Phe Ser Glu Ala Arg Val Glu Ile Val Met Thr Gln Ser Pro 
    130                 135                 140                 


Ala Thr Leu Ser Val Ser Pro Gly Glu Arg Ala Thr Leu Ser Cys Arg 
145                 150                 155                 160 


Ala Ser Gln Ser Val Ser Ser Asn Leu Ala Trp Tyr Gln Gln Lys Pro 
                165                 170                 175     


Gly Gln Ala Pro Arg Leu Ile Ile Tyr Gly Ala Ser Thr Thr Ala Ser 
            180                 185                 190         


Gly Ile Pro Ala Arg Phe Ser Ala Ser Gly Ser Gly Thr Asp Phe Thr 
        195                 200                 205             


Leu Thr Ile Ser Ser Leu Gln Ser Glu Asp Phe Ala Val Tyr Tyr Cys 
    210                 215                 220                 


Gln Gln Tyr Asn Asn Trp Pro Pro Ala Tyr Thr Phe Gly Gln Gly Thr 
225                 230                 235                 240 


Lys Leu Glu Ile Lys Glu Pro Lys Ser Cys Asp Lys Thr His Thr Cys 
                245                 250                 255     


Pro Pro Cys Pro Asp Pro Lys Gly Gly Thr Val Leu Leu Leu Leu Phe 
            260                 265                 270         


Val Ile Ser Ile Thr Thr Ile Ile Val Ile Phe Leu Arg Val Lys Phe 
        275                 280                 285             


Ser Arg Ser Ala Asp Ala Pro Ala Tyr Gln Gln Gly Gln Asn Gln Leu 
    290                 295                 300                 


Tyr Asn Glu Leu Asn Leu Gly Arg Arg Glu Glu Tyr Asp Val Leu Asp 
305                 310                 315                 320 


Lys Arg Arg Gly Arg Asp Pro Glu Met Gly Gly Lys Pro Arg Arg Lys 
                325                 330                 335     


Asn Pro Gln Glu Gly Leu Tyr Asn Glu Leu Gln Lys Asp Lys Met Ala 
            340                 345                 350         


Glu Ala Tyr Ser Glu Ile Gly Met Lys Gly Glu Arg Arg Arg Gly Lys 
        355                 360                 365             


Gly His Asp Gly Leu Tyr Gln Gly Leu Ser Thr Ala Thr Lys Asp Thr 
    370                 375                 380                 


Tyr Asp Ala Leu His Met Gln Ala Leu Pro Pro Arg 
385                 390                 395     


<210>  98
<211>  457
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  scFV 3-17I/hIgG1 hinge/CD226 TMD/CD226 ICD v1/CD3zeta

<400>  98

Gln Val Gln Leu Val Gln Ser Gly Ala Glu Val Lys Lys Pro Gly Ser 
1               5                   10                  15      


Ser Val Lys Val Ser Cys Lys Ala Ser Gly Gly Thr Phe Ser Ser Tyr 
            20                  25                  30          


Ala Ile Ser Trp Val Arg Gln Ala Pro Gly Gln Gly Leu Glu Trp Met 
        35                  40                  45              


Gly Gly Ile Ile Pro Ile Phe Gly Thr Ala Asn Tyr Ala Gln Lys Phe 
    50                  55                  60                  


Gln Gly Arg Val Thr Ile Thr Ala Asp Glu Ser Thr Ser Thr Ala Tyr 
65                  70                  75                  80  


Met Glu Leu Ser Ser Leu Arg Ser Glu Asp Thr Ala Val Tyr Tyr Cys 
                85                  90                  95      


Ala Arg Gly Leu Leu Trp Asn Tyr Trp Gly Gln Gly Thr Leu Val Thr 
            100                 105                 110         


Val Ser Ser Lys Leu Ser Gly Ser Ala Ser Ala Pro Lys Leu Glu Glu 
        115                 120                 125             


Gly Glu Phe Ser Glu Ala Arg Val Glu Ile Val Met Thr Gln Ser Pro 
    130                 135                 140                 


Ala Thr Leu Ser Val Ser Pro Gly Glu Arg Ala Thr Leu Ser Cys Arg 
145                 150                 155                 160 


Ala Ser Gln Ser Val Ser Ser Asn Leu Ala Trp Tyr Gln Gln Lys Pro 
                165                 170                 175     


Gly Gln Ala Pro Arg Leu Ile Ile Tyr Gly Ala Ser Thr Thr Ala Ser 
            180                 185                 190         


Gly Ile Pro Ala Arg Phe Ser Ala Ser Gly Ser Gly Thr Asp Phe Thr 
        195                 200                 205             


Leu Thr Ile Ser Ser Leu Gln Ser Glu Asp Phe Ala Val Tyr Tyr Cys 
    210                 215                 220                 


Gln Gln Tyr Asn Asn Trp Pro Pro Ala Tyr Thr Phe Gly Gln Gly Thr 
225                 230                 235                 240 


Lys Leu Glu Ile Lys Glu Pro Lys Ser Cys Asp Lys Thr His Thr Cys 
                245                 250                 255     


Pro Pro Cys Pro Asp Pro Lys Gly Gly Thr Val Leu Leu Leu Leu Phe 
            260                 265                 270         


Val Ile Ser Ile Thr Thr Ile Ile Val Ile Phe Leu Asn Arg Arg Arg 
        275                 280                 285             


Arg Arg Glu Arg Arg Asp Leu Phe Thr Glu Ser Trp Asp Thr Gln Lys 
    290                 295                 300                 


Ala Pro Asn Asn Tyr Arg Ser Pro Ile Ser Thr Ser Gln Pro Thr Asn 
305                 310                 315                 320 


Gln Ser Met Asp Asp Thr Arg Glu Asp Ile Tyr Val Asn Tyr Pro Thr 
                325                 330                 335     


Phe Ser Arg Arg Pro Lys Thr Arg Val Arg Val Lys Phe Ser Arg Ser 
            340                 345                 350         


Ala Asp Ala Pro Ala Tyr Gln Gln Gly Gln Asn Gln Leu Tyr Asn Glu 
        355                 360                 365             


Leu Asn Leu Gly Arg Arg Glu Glu Tyr Asp Val Leu Asp Lys Arg Arg 
    370                 375                 380                 


Gly Arg Asp Pro Glu Met Gly Gly Lys Pro Arg Arg Lys Asn Pro Gln 
385                 390                 395                 400 


Glu Gly Leu Tyr Asn Glu Leu Gln Lys Asp Lys Met Ala Glu Ala Tyr 
                405                 410                 415     


Ser Glu Ile Gly Met Lys Gly Glu Arg Arg Arg Gly Lys Gly His Asp 
            420                 425                 430         


Gly Leu Tyr Gln Gly Leu Ser Thr Ala Thr Lys Asp Thr Tyr Asp Ala 
        435                 440                 445             


Leu His Met Gln Ala Leu Pro Pro Arg 
    450                 455         


<210>  99
<211>  1254
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  (nucleotide) hIgG heavy chain signal sequence/scFV GC33/hIgG1 
       hinge/CD28 TMD/CD3zeta

<400>  99
atggactgga tctggaggat tttgttcctt gtgggagctg ccaccggtgc acattcgcaa       60

gtgcagttgc agcagtcggg agccgaactg gtccggcctg gagcttccgt gaagcttagc      120

tgcaaggcct ccggctacac ctttaccgac tacgagatgc actgggtcaa gcagaccccc      180

gtgcacggtc tgaagtggat tggggccctg gatcccaaga ccggcgatac tgcgtactca      240

cagaagttca agggaaaggc cacgctcact gcggacaaat cgtccagcac cgcgtacatg      300

gaactcagga gcctgacttc cgaggatagc gcagtgtact actgcacccg cttttactcc      360

tacacttact ggggacaggg caccttggtg actgtgtcag ccggtggagg cggatcaggg      420

ggtggaggat ccgggggagg aggatccgat gtggtcatga cccagacccc actgtccctt      480

cccgtgtccc tgggtgacca agcctcgatc agctgcagat cctcccagtc actggtccac      540

agcaacggca acacctatct gcattggtac ctccagaagc cgggacaatc ccccaagctc      600

ctgatctaca aggtgtccaa ccggttcagc ggagtgccgg atcgattctc agggtcgggt      660

tcgggaaccg acttcaccct taagatttcc cgggtggaag ccgaggatct cggagtgtac      720

ttctgctccc aaaataccca cgtgccgcct acattcggat cgggaactaa gctggagatc      780

aaggagccca aaagctgcga caagacccac acttgcccac cttgcccgga tccgaagttc      840

tgggtgctgg tggtggtcgg gggagtgctc gcctgctact ctctgctggt gaccgtggcc      900

ttcatcatcc gcgtgaagtt cagccggagc gctgatgccc ctgcatacca gcaggggcag      960

aaccagctct acaacgaact gaaccttgga cggcgggagg aatacgatgt gctggataag     1020

cgaagaggcc gcgacccaga aatgggcggg aagcccagac gcaagaatcc tcaggaggga     1080

ctgtacaacg agctccagaa agacaagatg gccgaagcgt acagcgagat cggcatgaag     1140

ggggaacgga gaaggggaaa gggccatgac ggattgtacc agggcctgtc gaccgctacc     1200

aaagacacct acgacgccct ccatatgcaa gcactgccgc cacgctgaac gcgt           1254


<210>  100
<211>  1107
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  (nucleotide) hIgG heavy chain signal sequence/scFV GC33/hIgG1 
       hinge/CD28 TMD/CD226 ICD v2

<400>  100
atggactgga tctggaggat tttgttcctt gtgggagctg ccaccggtgc acattcgcaa       60

gtgcagttgc agcagtcggg agccgaactg gtccggcctg gagcttccgt gaagcttagc      120

tgcaaggcct ccggctacac ctttaccgac tacgagatgc actgggtcaa gcagaccccc      180

gtgcacggtc tgaagtggat tggggccctg gatcccaaga ccggcgatac tgcgtactca      240

cagaagttca agggaaaggc cacgctcact gcggacaaat cgtccagcac cgcgtacatg      300

gaactcagga gcctgacttc cgaggatagc gcagtgtact actgcacccg cttttactcc      360

tacacttact ggggacaggg caccttggtg actgtgtcag ccggtggagg cggatcaggg      420

ggtggaggat ccgggggagg aggatccgat gtggtcatga cccagacccc actgtccctt      480

cccgtgtccc tgggtgacca agcctcgatc agctgcagat cctcccagtc actggtccac      540

agcaacggca acacctatct gcattggtac ctccagaagc cgggacaatc ccccaagctc      600

ctgatctaca aggtgtccaa ccggttcagc ggagtgccgg atcgattctc agggtcgggt      660

tcgggaaccg acttcaccct taagatttcc cgggtggaag ccgaggatct cggagtgtac      720

ttctgctccc aaaataccca cgtgccgcct acattcggat cgggaactaa gctggagatc      780

aaggagccca aaagctgcga caagacccac acttgcccac cttgtccgga tccgaagttc      840

tgggtgctgg tggtggtcgg gggagtgctc gcctgctact ctctgctggt gaccgtggca      900

ttcattatct tcctgaacag aaggaggcgc cgggagcggc gcgacctgtt cactgaatcc      960

tgggacaccc agaaggcccc caacaactac aggtccccta tctcaacctc ccaacccacc     1020

aaccagagca tggacgatac tcgcgaggac atctacgtga actaccccac tttctcccgg     1080

cggcctaaga cacgggtgtg aacgcgt                                         1107


<210>  101
<211>  1380
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  (nucleotide) hIgG heavy chain signal sequence/scFV GC33/hIgG1 
       hinge/CD28 TMD/41BB/CD3zeta

<400>  101
atggactgga tctggaggat tttgttcctt gtgggagctg ccaccggtgc acattcgcag       60

gtgcagttgc agcagtcggg agccgaactg gtccggcctg gagcttccgt gaagcttagc      120

tgcaaggcct ccggctacac ctttaccgac tacgagatgc actgggtcaa gcagaccccc      180

gtgcacggtc tgaagtggat tggggccctg gatcccaaga ccggcgatac tgcgtactca      240

cagaagttca agggaaaggc cacgctcact gcggacaaat cgtccagcac cgcgtacatg      300

gaactcagga gcctgacttc cgaggatagc gcagtgtact actgcacccg cttttactcc      360

tacacttact ggggacaggg caccttggtg actgtgtcag ccggtggagg cggatcaggg      420

ggtggaggat ccgggggagg aggatccgat gtggtcatga cccagacccc actgtccctt      480

cccgtgtccc tgggtgacca agcctcgatc agctgcagat cctcccagtc actggtccac      540

agcaacggca acacctatct gcattggtac ctccagaagc cgggacaatc ccccaagctc      600

ctgatctaca aggtgtccaa ccggttcagc ggagtgccgg atcgattctc agggtcgggt      660

tcgggaaccg acttcaccct taagatttcc cgggtggaag ccgaggatct cggagtgtac      720

ttctgctccc aaaataccca cgtgccgcct acattcggat cgggaactaa gctggagatc      780

aaggagccca aaagctgcga caagacccac acttgcccac cttgcccgga tccaaagttc      840

tgggtgctgg tggtggtcgg gggagtgctc gcctgctact ctctgctggt gaccgtggcc      900

tttataatca agcgcggtcg gaagaagctg ctgtacatct tcaagcagcc cttcatgcgg      960

cctgtgcaga ccacacagga agaggatggc tgctcctgcc gcttcccgga ggaagaggag     1020

ggcggatgcg aactgcgcgt gaagttcagc cggagcgctg atgcccctgc ataccagcag     1080

gggcagaacc agctctacaa cgaactgaac cttggacggc gggaggaata cgatgtgctg     1140

gataagcgaa gaggccgcga cccagaaatg ggcgggaagc ccagacgcaa gaatcctcag     1200

gagggactgt acaacgagct ccagaaagac aagatggccg aagcgtacag cgagatcggc     1260

atgaaggggg aacggagaag gggaaagggc catgacggat tgtaccaggg cctgtcgacc     1320

gctaccaaag acacctacga cgccctccat atgcaagcac tgccgccacg ctgaacgcgt     1380


<210>  102
<211>  1563
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  (nucleotide) hIgG heavy chain signal sequence/scFV GC33/hIgG1 
       hinge/CD28 TMD/ CD226 ICD v1/41BB/CD3zeta

<400>  102
atggactgga tctggaggat tttgttcctt gtgggagctg ccaccggtgc acattcgcaa       60

gtgcagttgc agcagtcggg agccgaactg gtccggcctg gagcttccgt gaagcttagc      120

tgcaaggcct ccggctacac ctttaccgac tacgagatgc actgggtcaa gcagaccccc      180

gtgcacggtc tgaagtggat tggggccctg gatcccaaga ccggcgatac tgcgtactca      240

cagaagttca agggaaaggc cacgctcact gcggacaaat cgtccagcac cgcgtacatg      300

gaactcagga gcctgacttc cgaggatagc gcagtgtact actgcacccg cttttactcc      360

tacacttact ggggacaggg caccttggtg actgtgtcag ccggtggagg cggatcaggg      420

ggtggaggat ccgggggagg aggatccgat gtggtcatga cccagacccc actgtccctt      480

cccgtgtccc tgggtgacca agcctcgatc agctgcagat cctcccagtc actggtccac      540

agcaacggca acacctatct gcattggtac ctccagaagc cgggacaatc ccccaagctc      600

ctgatctaca aggtgtccaa ccggttcagc ggagtgccgg atcgattctc agggtcgggt      660

tcgggaaccg acttcaccct taagatttcc cgggtggaag ccgaggatct cggagtgtac      720

ttctgctccc aaaataccca cgtgccgcct acattcggat cgggaactaa gctggagatc      780

aaggagccca aaagctgcga caagacccac acttgcccac cttgtccgga tccgaagttc      840

tgggtgctgg tggtggtcgg gggagtgctc gcctgctact ctctgctggt gaccgtggca      900

ttcattatca acagaaggag gcgccgggag cggcgcgacc tgttcactga atcctgggac      960

acccagaagg cccccaacaa ctacaggtcc cctatctcaa cctcccaacc caccaaccag     1020

agcatggacg atactcgcga ggacatctac gtgaactacc ccactttctc ccggcggcct     1080

aagacccgcg tgaagcgcgg tcggaagaag ctgctgtaca tcttcaagca gcccttcatg     1140

cggcctgtgc agaccacaca ggaagaggat ggctgctcct gccgcttccc ggaggaagag     1200

gagggcggat gcgaactgcg cgtgaagttc agccggagcg ctgatgcccc tgcataccag     1260

caggggcaga accagctcta caacgaactg aaccttggac ggcgggagga atacgatgtg     1320

ctggataagc gaagaggccg cgacccagaa atgggcggga agcccagacg caagaatcct     1380

caggagggac tgtacaacga gctccagaaa gacaagatgg ccgaagcgta cagcgagatc     1440

ggcatgaagg gggaacggag aaggggaaag ggccatgacg gattgtacca gggcctgtcg     1500

accgctacca aagacaccta cgacgccctc catatgcaag cactgccgcc tagatgaacg     1560

cgt                                                                   1563


<210>  103
<211>  1569
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  (nucleotide) hIgG heavy chain signal sequence/scFV GC33/hIgG1 
       hinge/CD28 TMD/ CD226 ICD v2/41BB/CD3zeta

<400>  103
atggactgga tctggaggat tttgttcctt gtgggagctg ccaccggtgc acattcgcaa       60

gtgcagttgc agcagtcggg agccgaactg gtccggcctg gagcttccgt gaagcttagc      120

tgcaaggcct ccggctacac ctttaccgac tacgagatgc actgggtcaa gcagaccccc      180

gtgcacggtc tgaagtggat tggggccctg gatcccaaga ccggcgatac tgcgtactca      240

cagaagttca agggaaaggc cacgctcact gcggacaaat cgtccagcac cgcgtacatg      300

gaactcagga gcctgacttc cgaggatagc gcagtgtact actgcacccg cttttactcc      360

tacacttact ggggacaggg caccttggtg actgtgtcag ccggtggagg cggatcaggg      420

ggtggaggat ccgggggagg aggatccgat gtggtcatga cccagacccc actgtccctt      480

cccgtgtccc tgggtgacca agcctcgatc agctgcagat cctcccagtc actggtccac      540

agcaacggca acacctatct gcattggtac ctccagaagc cgggacaatc ccccaagctc      600

ctgatctaca aggtgtccaa ccggttcagc ggagtgccgg atcgattctc agggtcgggt      660

tcgggaaccg acttcaccct taagatttcc cgggtggaag ccgaggatct cggagtgtac      720

ttctgctccc aaaataccca cgtgccgcct acattcggat cgggaactaa gctggagatc      780

aaggagccca aaagctgcga caagacccac acttgcccac cttgtccgga tccgaagttc      840

tgggtgctgg tggtggtcgg gggagtgctc gcctgctact ctctgctggt gaccgtggca      900

ttcattatct tcctgaacag aaggaggcgc cgggagcggc gcgacctgtt cactgaatcc      960

tgggacaccc agaaggcccc caacaactac aggtccccta tctcaacctc ccaacccacc     1020

aaccagagca tggacgatac tcgcgaggac atctacgtga actaccccac tttctcccgg     1080

cggcctaaga cccgcgtgaa gcgcggtcgg aagaagctgc tgtacatctt caagcagccc     1140

ttcatgcggc ctgtgcagac cacacaggaa gaggatggct gctcctgccg cttcccggag     1200

gaagaggagg gcggatgcga actgcgcgtg aagttcagcc ggagcgctga tgcccctgca     1260

taccagcagg ggcagaacca gctctacaac gaactgaacc ttggacggcg ggaggaatac     1320

gatgtgctgg ataagcgaag aggccgcgac ccagaaatgg gcgggaagcc cagacgcaag     1380

aatcctcagg agggactgta caacgagctc cagaaagaca agatggccga agcgtacagc     1440

gagatcggca tgaaggggga acggagaagg ggaaagggcc atgacggatt gtaccagggc     1500

ctgtcgaccg ctaccaaaga cacctacgac gccctccata tgcaagcact gccgcctaga     1560

tgaacgcgt                                                             1569


<210>  104
<211>  1434
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  (nucleotide) hIgG heavy chain signal sequence/scFV GC33/hIgG1 
       hinge/CD28 TMD/ CD226 ICD v1/CD3zeta

<400>  104
atggactgga tctggaggat tttgttcctt gtgggagctg ccaccggtgc acattcgcaa       60

gtgcagttgc agcagtcggg agccgaactg gtccggcctg gagcttccgt gaagcttagc      120

tgcaaggcct ccggctacac ctttaccgac tacgagatgc actgggtcaa gcagaccccc      180

gtgcacggtc tgaagtggat tggggccctg gatcccaaga ccggcgatac tgcgtactca      240

cagaagttca agggaaaggc cacgctcact gcggacaaat cgtccagcac cgcgtacatg      300

gaactcagga gcctgacttc cgaggatagc gcagtgtact actgcacccg cttttactcc      360

tacacttact ggggacaggg caccttggtg actgtgtcag ccggtggagg cggatcaggg      420

ggtggaggat ccgggggagg aggatccgat gtggtcatga cccagacccc actgtccctt      480

cccgtgtccc tgggtgacca agcctcgatc agctgcagat cctcccagtc actggtccac      540

agcaacggca acacctatct gcattggtac ctccagaagc cgggacaatc ccccaagctc      600

ctgatctaca aggtgtccaa ccggttcagc ggagtgccgg atcgattctc agggtcgggt      660

tcgggaaccg acttcaccct taagatttcc cgggtggaag ccgaggatct cggagtgtac      720

ttctgctccc aaaataccca cgtgccgcct acattcggat cgggaactaa gctggagatc      780

aaggagccca aaagctgcga caagacccac acttgcccac cttgcccgga tccgaagttt      840

tgggtcttgg tggtggtggg aggcgtcctc gcctgctaca gcttgctcgt gaccgttgcc      900

ttcatcatca accgcaggcg gagaagggaa cggcgcgacc tgttcactga gtcatgggac      960

acccagaagg ccccgaacaa ctaccgctcc ccgatctcca cctcccaacc gactaatcaa     1020

agcatggacg acaccaggga ggacatctac gtgaactacc ctactttctc ccgccggcct     1080

aagactcggg tgcgcgtgaa gttcagccgg agcgctgatg cccctgcata ccagcagggg     1140

cagaaccagc tctacaacga actgaacctt ggacggcggg aggaatacga tgtgctggat     1200

aagcgaagag gccgcgaccc agaaatgggc gggaagccca gacgcaagaa tcctcaggag     1260

ggactgtaca acgagctcca gaaagacaag atggccgaag cgtacagcga gatcggcatg     1320

aagggggaac ggagaagggg aaagggccat gacggattgt accagggcct gtcgaccgct     1380

accaaagaca cctacgacgc cctccatatg caagcactgc cgccacgctg atag           1434


<210>  105
<211>  1440
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  (nucleotide) hIgG heavy chain signal sequence/scFV GC33/hIgG1 
       hinge/CD28 TMD/ CD226 ICD v2/CD3zeta

<400>  105
atggactgga tctggaggat tttgttcctt gtgggagctg ccaccggtgc acattcgcaa       60

gtgcagttgc agcagtcggg agccgaactg gtccggcctg gagcttccgt gaagcttagc      120

tgcaaggcct ccggctacac ctttaccgac tacgagatgc actgggtcaa gcagaccccc      180

gtgcacggtc tgaagtggat tggggccctg gatcccaaga ccggcgatac tgcgtactca      240

cagaagttca agggaaaggc cacgctcact gcggacaaat cgtccagcac cgcgtacatg      300

gaactcagga gcctgacttc cgaggatagc gcagtgtact actgcacccg cttttactcc      360

tacacttact ggggacaggg caccttggtg actgtgtcag ccggtggagg cggatcaggg      420

ggtggaggat ccgggggagg aggatccgat gtggtcatga cccagacccc actgtccctt      480

cccgtgtccc tgggtgacca agcctcgatc agctgcagat cctcccagtc actggtccac      540

agcaacggca acacctatct gcattggtac ctccagaagc cgggacaatc ccccaagctc      600

ctgatctaca aggtgtccaa ccggttcagc ggagtgccgg atcgattctc agggtcgggt      660

tcgggaaccg acttcaccct taagatttcc cgggtggaag ccgaggatct cggagtgtac      720

ttctgctccc aaaataccca cgtgccgcct acattcggat cgggaactaa gctggagatc      780

aaggagccca aaagctgcga caagacccac acttgcccac cttgcccgga tccgaagttc      840

tgggtgttgg tcgtggtcgg aggcgtcctc gcctgctaca gcctgctcgt gaccgtggcc      900

ttcatcatct tcctgaaccg caggcggaga agggaacggc gcgacctgtt cactgagtca      960

tgggacaccc agaaggcccc gaacaactac cgctccccga tctccacctc ccaaccgact     1020

aatcaaagca tggacgacac cagggaggac atctacgtga actaccctac tttctcccgc     1080

cggcctaaga ctcgggtgcg cgtgaagttc agccggagcg ctgatgcccc tgcataccag     1140

caggggcaga accagctcta caacgaactg aaccttggac ggcgggagga atacgatgtg     1200

ctggataagc gaagaggccg cgacccagaa atgggcggga agcccagacg caagaatcct     1260

caggagggac tgtacaacga gctccagaaa gacaagatgg ccgaagcgta cagcgagatc     1320

ggcatgaagg gggaacggag aaggggaaag ggccatgacg gattgtacca gggcctgtcg     1380

accgctacca aagacaccta cgacgccctc catatgcaag cactgccgcc acgctgatag     1440


<210>  106
<211>  1383
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  (nucleotide) hIgG heavy chain signal sequence/scFV GC33/hIgG1 
       hinge/CD28 TMD/ CD28 ICD/CD3zeta

<400>  106
atggactgga tctggaggat tttgttcctt gtgggagctg ccaccggtgc acattcgcaa       60

gtgcagttgc agcagtcggg agccgaactg gtccggcctg gagcttccgt gaagcttagc      120

tgcaaggcct ccggctacac ctttaccgac tacgagatgc actgggtcaa gcagaccccc      180

gtgcacggtc tgaagtggat tggggccctg gatcccaaga ccggcgatac tgcgtactca      240

cagaagttca agggaaaggc cacgctcact gcggacaaat cgtccagcac cgcgtacatg      300

gaactcagga gcctgacttc cgaggatagc gcagtgtact actgcacccg cttttactcc      360

tacacttact ggggacaggg caccttggtg actgtgtcag ccggtggagg cggatcaggg      420

ggtggaggat ccgggggagg aggatccgat gtggtcatga cccagacccc actgtccctt      480

cccgtgtccc tgggtgacca agcctcgatc agctgcagat cctcccagtc actggtccac      540

agcaacggca acacctatct gcattggtac ctccagaagc cgggacaatc ccccaagctc      600

ctgatctaca aggtgtccaa ccggttcagc ggagtgccgg atcgattctc agggtcgggt      660

tcgggaaccg acttcaccct taagatttcc cgggtggaag ccgaggatct cggagtgtac      720

ttctgctccc aaaataccca cgtgccgcct acattcggat cgggaactaa gctggagatc      780

aaggagccca aaagctgcga caagacccac acttgcccac cttgcccgga tccgaagttc      840

tgggtcttgg tggtcgtggg aggcgtcctc gcctgctaca gcctcctcgt gaccgtcgcc      900

ttcatcatct tctgggtgcg ctccaagcgc tccagactgc tgcacagcga ctacatgaac      960

atgaccccaa gacgcccagg acctactagg aagcattatc aaccttatgc cccgccgaga     1020

gacttcgcgg cgtaccggtc ccgcgtgaag ttcagccgga gcgctgatgc ccctgcatac     1080

cagcaggggc agaaccagct ctacaacgaa ctgaaccttg gacggcggga ggaatacgat     1140

gtgctggata agcgaagagg ccgcgaccca gaaatgggcg ggaagcccag acgcaagaat     1200

cctcaggagg gactgtacaa cgagctccag aaagacaaga tggccgaagc gtacagcgag     1260

atcggcatga agggggaacg gagaagggga aagggccatg acggattgta ccagggcctg     1320

tcgaccgcta ccaaagacac ctacgacgcc ctccatatgc aagcactgcc gccacgctga     1380

tag                                                                   1383


<210>  107
<211>  1566
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  (nucleotide) hIgG heavy chain signal sequence/scFV GC33/hIgG1 
       hinge/CD28 TMD/ CD226 ICD v1/ CD28 ICD/CD3zeta

<400>  107
atggactgga tctggaggat tttgttcctt gtgggagctg ccaccggtgc acattcgcaa       60

gtgcagttgc agcagtcggg agccgaactg gtccggcctg gagcttccgt gaagcttagc      120

tgcaaggcct ccggctacac ctttaccgac tacgagatgc actgggtcaa gcagaccccc      180

gtgcacggtc tgaagtggat tggggccctg gatcccaaga ccggcgatac tgcgtactca      240

cagaagttca agggaaaggc cacgctcact gcggacaaat cgtccagcac cgcgtacatg      300

gaactcagga gcctgacttc cgaggatagc gcagtgtact actgcacccg cttttactcc      360

tacacttact ggggacaggg caccttggtg actgtgtcag ccggtggagg cggatcaggg      420

ggtggaggat ccgggggagg aggatccgat gtggtcatga cccagacccc actgtccctt      480

cccgtgtccc tgggtgacca agcctcgatc agctgcagat cctcccagtc actggtccac      540

agcaacggca acacctatct gcattggtac ctccagaagc cgggacaatc ccccaagctc      600

ctgatctaca aggtgtccaa ccggttcagc ggagtgccgg atcgattctc agggtcgggt      660

tcgggaaccg acttcaccct taagatttcc cgggtggaag ccgaggatct cggagtgtac      720

ttctgctccc aaaataccca cgtgccgcct acattcggat cgggaactaa gctggagatc      780

aaggagccca aaagctgcga caagacccac acttgcccac cttgcccgga tccgaagttt      840

tgggtgttgg tggtcgtggg aggcgtcctc gcgtgctaca gcctcctcgt gaccgtcgcc      900

ttcatcatca accgcaggcg gagaagggaa cggcgcgacc tgttcactga gtcatgggac      960

acccagaagg ccccgaacaa ctaccgctcc ccgatctcca cctcccaacc gactaatcaa     1020

agcatggacg acaccaggga ggacatctac gtgaactacc ctactttctc ccgccggcct     1080

aagacccgcg tgttctgggt gcgctccaag cgctccagac tgctgcacag cgactacatg     1140

aacatgaccc caagacgccc aggacctact aggaagcatt atcaacctta tgccccgccg     1200

agagacttcg cggcgtaccg gtcccgcgtg aagttcagcc ggagcgctga tgcccctgca     1260

taccagcagg ggcagaacca gctctacaac gaactgaacc ttggacggcg ggaggaatac     1320

gatgtgctgg ataagcgaag aggccgcgac ccagaaatgg gcgggaagcc cagacgcaag     1380

aatcctcagg agggactgta caacgagctc cagaaagaca agatggccga agcgtacagc     1440

gagatcggca tgaaggggga acggagaagg ggaaagggcc atgacggatt gtaccagggc     1500

ctgtcgaccg ctaccaaaga cacctacgac gccctccata tgcaagcact gccgccacgc     1560

tgatag                                                                1566


<210>  108
<211>  1572
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  (nucleotide) hIgG heavy chain signal sequence/scFV GC33/hIgG1 
       hinge/CD28 TMD/ CD226 ICD v2/ CD28 ICD/CD3zeta

<400>  108
atggactgga tctggaggat tttgttcctt gtgggagctg ccaccggtgc acattcgcaa       60

gtgcagttgc agcagtcggg agccgaactg gtccggcctg gagcttccgt gaagcttagc      120

tgcaaggcct ccggctacac ctttaccgac tacgagatgc actgggtcaa gcagaccccc      180

gtgcacggtc tgaagtggat tggggccctg gatcccaaga ccggcgatac tgcgtactca      240

cagaagttca agggaaaggc cacgctcact gcggacaaat cgtccagcac cgcgtacatg      300

gaactcagga gcctgacttc cgaggatagc gcagtgtact actgcacccg cttttactcc      360

tacacttact ggggacaggg caccttggtg actgtgtcag ccggtggagg cggatcaggg      420

ggtggaggat ccgggggagg aggatccgat gtggtcatga cccagacccc actgtccctt      480

cccgtgtccc tgggtgacca agcctcgatc agctgcagat cctcccagtc actggtccac      540

agcaacggca acacctatct gcattggtac ctccagaagc cgggacaatc ccccaagctc      600

ctgatctaca aggtgtccaa ccggttcagc ggagtgccgg atcgattctc agggtcgggt      660

tcgggaaccg acttcaccct taagatttcc cgggtggaag ccgaggatct cggagtgtac      720

ttctgctccc aaaataccca cgtgccgcct acattcggat cgggaactaa gctggagatc      780

aaggagccca aaagctgcga caagacccac acttgcccac cttgcccgga tccgaagttc      840

tgggtcttgg tggtggtcgg aggcgtcctc gcttgttaca gcctgctcgt gaccgttgcc      900

ttcatcatct tcctgaaccg caggcggaga agggaacggc gcgacctgtt cactgagtca      960

tgggacaccc agaaggcccc gaacaactac cgctccccga tctccacctc ccaaccgact     1020

aatcaaagca tggacgacac cagggaggac atctacgtga actaccctac tttctcccgc     1080

cggcctaaga cccgcgtgtt ctgggtgcgc tccaagcgct ccagactgct gcacagcgac     1140

tacatgaaca tgaccccaag acgcccagga cctactagga agcattatca accttatgcc     1200

ccgccgagag acttcgcggc gtaccggtcc cgcgtgaagt tcagccggag cgctgatgcc     1260

cctgcatacc agcaggggca gaaccagctc tacaacgaac tgaaccttgg acggcgggag     1320

gaatacgatg tgctggataa gcgaagaggc cgcgacccag aaatgggcgg gaagcccaga     1380

cgcaagaatc ctcaggaggg actgtacaac gagctccaga aagacaagat ggccgaagcg     1440

tacagcgaga tcggcatgaa gggggaacgg agaaggggaa agggccatga cggattgtac     1500

cagggcctgt cgaccgctac caaagacacc tacgacgccc tccatatgca agcactgccg     1560

ccacgctgat ag                                                         1572


<210>  109
<211>  1566
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  (nucleotide) hIgG heavy chain signal sequence/scFV GC33/hIgG1 
       hinge/CD28 TMD/ CD28 ICD/CD226 ICD v1/CD3zeta

<400>  109
atggactgga tctggaggat tttgttcctt gtgggagctg ccaccggtgc acattcgcaa       60

gtgcagttgc agcagtcggg agccgaactg gtccggcctg gagcttccgt gaagcttagc      120

tgcaaggcct ccggctacac ctttaccgac tacgagatgc actgggtcaa gcagaccccc      180

gtgcacggtc tgaagtggat tggggccctg gatcccaaga ccggcgatac tgcgtactca      240

cagaagttca agggaaaggc cacgctcact gcggacaaat cgtccagcac cgcgtacatg      300

gaactcagga gcctgacttc cgaggatagc gcagtgtact actgcacccg cttttactcc      360

tacacttact ggggacaggg caccttggtg actgtgtcag ccggtggagg cggatcaggg      420

ggtggaggat ccgggggagg aggatccgat gtggtcatga cccagacccc actgtccctt      480

cccgtgtccc tgggtgacca agcctcgatc agctgcagat cctcccagtc actggtccac      540

agcaacggca acacctatct gcattggtac ctccagaagc cgggacaatc ccccaagctc      600

ctgatctaca aggtgtccaa ccggttcagc ggagtgccgg atcgattctc agggtcgggt      660

tcgggaaccg acttcaccct taagatttcc cgggtggaag ccgaggatct cggagtgtac      720

ttctgctccc aaaataccca cgtgccgcct acattcggat cgggaactaa gctggagatc      780

aaggagccca aaagctgcga caagacccac acttgcccac cttgcccgga tccgaagttc      840

tgggtgttgg tggtcgtggg aggcgtcctc gcatgttact ccctgctggt gactgtggcc      900

ttcatcattt tttgggtgag gtccaagcgg tcgcggctgc tgcactccga ctacatgaac      960

atgaccccca gaagacccgg tcctacacgg aagcattacc aaccctacgc ccccccgagg     1020

gacttcgccg cctaccggtc caaccgcagg cggagaaggg aacggcgcga cctgttcact     1080

gagtcatggg acacccagaa ggccccgaac aactaccgct ccccgatctc cacctcccaa     1140

ccgactaatc aaagcatgga cgacaccagg gaggacatct acgtgaacta ccctactttc     1200

tcccgccggc ctaagacccg cgtgcgcgtg aagttcagcc ggagcgctga tgcccctgca     1260

taccagcagg ggcagaacca gctctacaac gaactgaacc ttggacggcg ggaggaatac     1320

gatgtgctgg ataagcgaag aggccgcgac ccagaaatgg gcgggaagcc cagacgcaag     1380

aatcctcagg agggactgta caacgagctc cagaaagaca agatggccga agcgtacagc     1440

gagatcggca tgaaggggga acggagaagg ggaaagggcc atgacggatt gtaccagggc     1500

ctgtcgaccg ctaccaaaga cacctacgac gccctccata tgcaagcact gccgccacgc     1560

tgatag                                                                1566


<210>  110
<211>  1509
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  (nucleotide) hIgG heavy chain signal sequence/scFV GC33/hIgG1 
       hinge/CD28 TMD/ CD28 ICD/41BB/CD3zeta

<400>  110
atggactgga tctggaggat tttgttcctt gtgggagctg ccaccggtgc acattcgcaa       60

gtgcagttgc agcagtcggg agccgaactg gtccggcctg gagcttccgt gaagcttagc      120

tgcaaggcct ccggctacac ctttaccgac tacgagatgc actgggtcaa gcagaccccc      180

gtgcacggtc tgaagtggat tggggccctg gatcccaaga ccggcgatac tgcgtactca      240

cagaagttca agggaaaggc cacgctcact gcggacaaat cgtccagcac cgcgtacatg      300

gaactcagga gcctgacttc cgaggatagc gcagtgtact actgcacccg cttttactcc      360

tacacttact ggggacaggg caccttggtg actgtgtcag ccggtggagg cggatcaggg      420

ggtggaggat ccgggggagg aggatccgat gtggtcatga cccagacccc actgtccctt      480

cccgtgtccc tgggtgacca agcctcgatc agctgcagat cctcccagtc actggtccac      540

agcaacggca acacctatct gcattggtac ctccagaagc cgggacaatc ccccaagctc      600

ctgatctaca aggtgtccaa ccggttcagc ggagtgccgg atcgattctc agggtcgggt      660

tcgggaaccg acttcaccct taagatttcc cgggtggaag ccgaggatct cggagtgtac      720

ttctgctccc aaaataccca cgtgccgcct acattcggat cgggaactaa gctggagatc      780

aaggagccca aaagctgcga caagacccac acttgcccac cttgcccgga tccgaagttc      840

tgggtgctgg tggtggtcgg gggagtgctc gcctgctact ctctgctggt gaccgtggcc      900

ttcatcatct tttgggtgag atccaagcgg tctcgcctgc tgcacagcga ctacatgaac      960

atgaccccgc gcagacctgg cccgactagg aagcactacc agccctacgc cccccccagg     1020

gatttcgccg cctacagatc caagcgcggt cggaagaagc tgctgtacat cttcaagcag     1080

cccttcatgc ggcctgtgca gaccacacag gaagaggatg gctgctcctg ccgcttcccg     1140

gaggaagagg agggcggatg cgaactgcgc gtgaagttca gccggagcgc tgatgcccct     1200

gcataccagc aggggcagaa ccagctctac aacgaactga accttggacg gcgggaggaa     1260

tacgatgtgc tggataagcg aagaggccgc gacccagaaa tgggcgggaa gcccagacgc     1320

aagaatcctc aggagggact gtacaacgag ctccagaaag acaagatggc cgaagcgtac     1380

agcgagatcg gcatgaaggg ggaacggaga aggggaaagg gccatgacgg attgtaccag     1440

ggcctgtcga ccgctaccaa agacacctac gacgccctcc atatgcaagc actgccgcca     1500

cgctgatag                                                             1509


<210>  111
<211>  1692
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  (nucleotide) hIgG heavy chain signal sequence/scFV GC33/hIgG1 
       hinge/CD28 TMD/ CD226 ICD v1/ CD28 ICD/41BB/CD3zeta

<400>  111
atggactgga tctggaggat tttgttcctt gtgggagctg ccaccggtgc acattcgcaa       60

gtgcagttgc agcagtcggg agccgaactg gtccggcctg gagcttccgt gaagcttagc      120

tgcaaggcct ccggctacac ctttaccgac tacgagatgc actgggtcaa gcagaccccc      180

gtgcacggtc tgaagtggat tggggccctg gatcccaaga ccggcgatac tgcgtactca      240

cagaagttca agggaaaggc cacgctcact gcggacaaat cgtccagcac cgcgtacatg      300

gaactcagga gcctgacttc cgaggacagc gcagtgtact actgcacccg cttttactcc      360

tacacttact ggggacaggg caccttggtg actgtgtcag ccggtggagg cggatcaggg      420

ggtggaggat ccgggggagg aggatccgat gtggtcatga cccagacccc actgtccctt      480

cccgtgtccc tgggtgacca agcctcgatc agctgcagat cctcccagtc actggtccac      540

agcaacggca acacctatct gcattggtac ctccagaagc cgggacaatc ccccaagctc      600

ctgatctaca aggtgtccaa ccggttcagc ggagtgccgg atcgattctc agggtcgggt      660

tcgggaaccg acttcaccct taagatttcc cgggtggaag ccgaggatct cggagtgtac      720

ttctgctccc aaaataccca cgtgccgcct acattcggat cgggaactaa gctggagatc      780

aaggagccca aaagctgcga caagacccac acttgcccac cttgcccgga tccgaagttt      840

tgggtgttgg tggtggtcgg aggcgtcctc gcatgctata gcctgctcgt gaccgtggcc      900

ttcatcatca accgcaggcg gagaagggaa cggcgcgacc tgttcactga gtcatgggac      960

acccagaagg ccccgaacaa ctaccgctcc ccgatctcca cctcccaacc gactaatcaa     1020

agcatggacg acaccaggga ggacatctac gtgaactacc ctactttctc ccgccggcct     1080

aagacccgcg tgttctgggt gcgctccaag cgctccagac tgctgcacag cgactacatg     1140

aacatgaccc caagacgccc aggacctact aggaagcatt atcaacctta tgccccgccg     1200

agagacttcg cggcgtatag gtccaagcgc ggtcggaaga agctgctgta catcttcaag     1260

cagcccttca tgcggcctgt gcagaccaca caggaagagg atggctgctc ctgccgcttc     1320

ccggaggaag aggagggcgg atgcgaactg cgcgtgaagt tcagccggag cgctgatgcc     1380

cctgcatacc agcaggggca gaaccagctc tacaacgaac tgaaccttgg acggcgggag     1440

gaatacgatg tgctggataa gcgaagaggc cgcgacccag aaatgggcgg gaagcccaga     1500

cgcaagaatc ctcaggaggg actgtacaac gagctccaga aagacaagat ggccgaagcg     1560

tacagcgaga tcggcatgaa gggggaacgg agaaggggaa agggccatga cggattgtac     1620

cagggcctgt cgaccgctac caaagacacc tacgacgccc tccatatgca agcactgccg     1680

ccacgctgat ag                                                         1692


<210>  112
<211>  1698
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  (nucleotide) hIgG heavy chain signal sequence/scFV GC33/hIgG1 
       hinge/CD28 TMD/ CD226 ICD v2/ CD28 ICD/41BB/CD3zeta

<400>  112
atggactgga tctggaggat tttgttcctt gtgggagctg ccaccggtgc acattcgcaa       60

gtgcagttgc agcagtcggg agccgaactg gtccggcctg gagcttccgt gaagcttagc      120

tgcaaggcct ccggctacac ctttaccgac tacgagatgc actgggtcaa gcagaccccc      180

gtgcacggtc tgaagtggat tggggccctg gatcccaaga ccggcgatac tgcgtactca      240

cagaagttca agggaaaggc cacgctcact gcggacaaat cgtccagcac cgcgtacatg      300

gaactcagga gcctgacttc cgaggatagc gcagtgtact actgcacccg cttttactcc      360

tacacttact ggggacaggg caccttggtg actgtgtcag ccggtggagg cggatcaggg      420

ggtggaggat ccgggggagg aggatccgat gtggtcatga cccagacccc actgtccctt      480

cccgtgtccc tgggtgacca agcctcgatc agctgcagat cctcccagtc actggtccac      540

agcaacggca acacctatct gcattggtac ctccagaagc cgggacaatc ccccaagctc      600

ctgatctaca aggtgtccaa ccggttcagc ggagtgccgg atcgattctc agggtcgggt      660

tcgggaaccg acttcaccct taagatttcc cgggtggaag ccgaggatct cggagtgtac      720

ttctgctccc aaaataccca cgtgccgcct acattcggat cgggaactaa gctggagatc      780

aaggagccca aaagctgcga caagacccac acttgcccac cttgcccgga tccgaagttc      840

tgggtcttgg tggtggtcgg aggcgtcctc gcttgctaca gcctgctcgt gaccgtggcc      900

ttcatcatct tcctgaaccg caggcggaga agggaacggc gcgacctgtt cactgagtca      960

tgggacaccc agaaggcccc gaacaactac cgctccccga tctccacctc ccaaccgact     1020

aatcaaagca tggacgacac cagggaggac atctacgtga actaccctac tttctcccgc     1080

cggcctaaga cccgcgtgtt ctgggtgcgc tccaagcgct ccagactgct gcacagcgac     1140

tacatgaaca tgaccccaag acgcccagga cctactagga agcattatca accttatgcc     1200

ccgccgagag acttcgcggc gtataggtcc aagcgcggtc ggaagaagct gctgtacatc     1260

ttcaagcagc ccttcatgcg gcctgtgcag accacacagg aagaggatgg ctgctcctgc     1320

cgcttcccgg aggaagagga gggcggatgc gaactgcgcg tgaagttcag ccggagcgct     1380

gatgcccctg cataccagca ggggcagaac cagctctaca acgaactgaa ccttggacgg     1440

cgggaggaat acgatgtgct ggataagcga agaggccgcg acccagaaat gggcgggaag     1500

cccagacgca agaatcctca ggagggactg tacaacgagc tccagaaaga caagatggcc     1560

gaagcgtaca gcgagatcgg catgaagggg gaacggagaa ggggaaaggg ccatgacgga     1620

ttgtaccagg gcctgtcgac cgctaccaaa gacacctacg acgccctcca tatgcaagca     1680

ctgccgccac gctgatag                                                   1698


<210>  113
<211>  1692
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  (nucleotide) hIgG heavy chain signal sequence/scFV GC33/hIgG1 
       hinge/CD28 TMD/ CD28 ICD/CD226 ICD v1/41BB/CD3zeta

<400>  113
atggactgga tctggaggat tttgttcctt gtgggagctg ccaccggtgc acattcgcaa       60

gtgcagttgc agcagtcggg agccgaactg gtccggcctg gagcttccgt gaagcttagc      120

tgcaaggcct ccggctacac ctttaccgac tacgagatgc actgggtcaa gcagaccccc      180

gtgcacggtc tgaagtggat tggggccctg gatcccaaga ccggcgatac tgcgtactca      240

cagaagttca agggaaaggc cacgctcact gcggacaaat cgtccagcac cgcgtacatg      300

gaactcagga gcctgacttc cgaggatagc gcagtgtact actgcacccg cttttactcc      360

tacacttact ggggacaggg caccttggtg actgtgtcag ccggtggagg cggatcaggg      420

ggtggaggat ccgggggagg aggatccgat gtggtcatga cccagacccc actgtccctt      480

cccgtgtccc tgggtgacca agcctcgatc agctgcagat cctcccagtc actggtccac      540

agcaacggca acacctatct gcattggtac ctccagaagc cgggacaatc ccccaagctc      600

ctgatctaca aggtgtccaa ccggttcagc ggagtgccgg atcgattctc agggtcgggt      660

tcgggaaccg acttcaccct taagatttcc cgggtggaag ccgaggatct cggagtgtac      720

ttctgctccc aaaataccca cgtgccgcct acattcggat cgggaactaa gctggagatc      780

aaggagccca aaagctgcga caagacccac acttgcccac cttgtccgga tccgaagttc      840

tgggtgctgg tggtggtcgg gggagtgctc gcctgctact ctctgctggt gaccgtggca      900

ttcattatct tctgggtccg ctccaaacgg tcccggctgc tgcactccga ctacatgaac      960

atgaccccga gacggccagg accgactcgc aagcactacc agccgtacgc cccaccgaga     1020

gacttcgccg cataccgctc aaacagaagg aggcgccggg agcggcgcga cctgttcact     1080

gaatcctggg acacccagaa ggcccccaac aactacaggt cccctatctc aacctcccaa     1140

cccaccaacc agagcatgga cgatactcgc gaggacatct acgtgaacta ccccactttc     1200

tcccggcggc ctaagacccg cgtgaagcgc ggtcggaaga agctgctgta catcttcaag     1260

cagcccttca tgcggcctgt gcagaccaca caggaagagg atggctgctc ctgccgcttc     1320

ccggaggaag aggagggcgg atgcgaactg cgcgtgaagt tcagccggag cgctgatgcc     1380

cctgcatacc agcaggggca gaaccagctc tacaacgaac tgaaccttgg acggcgggag     1440

gaatacgatg tgctggataa gcgaagaggc cgcgacccag aaatgggcgg gaagcccaga     1500

cgcaagaatc ctcaggaggg actgtacaac gagctccaga aagacaagat ggccgaagcg     1560

tacagcgaga tcggcatgaa gggggaacgg agaaggggaa agggccatga cggattgtac     1620

cagggcctgt cgaccgctac caaagacacc tacgacgccc tccatatgca agcactgccg     1680

cctagatgat ag                                                         1692


<210>  114
<211>  1554
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  (nucleotide) hIgG heavy chain signal sequence/scFV GC33/hIgG1 
       hinge/CD226 TMD/CD226 ICD v1/41BB/CD3zeta

<400>  114
atggactgga tctggaggat tttgttcctt gtgggagctg ccaccggtgc acattcgcaa       60

gtgcagttgc agcagtcggg agccgaactg gtccggcctg gagcttccgt gaagcttagc      120

tgcaaggcct ccggctacac ctttaccgac tacgagatgc actgggtcaa gcagaccccc      180

gtgcacggtc tgaagtggat tggggccctg gatcccaaga ccggcgatac tgcgtactca      240

cagaagttca agggaaaggc cacgctcact gcggacaaat cgtccagcac cgcgtacatg      300

gaactcagga gcctgacttc cgaggatagc gcagtgtact actgcacccg cttttactcc      360

tacacttact ggggacaggg caccttggtg actgtgtcag ccggtggagg cggatcaggg      420

ggtggaggat ccgggggagg aggatccgat gtggtcatga cccagacccc actgtccctt      480

cccgtgtccc tgggtgacca agcctcgatc agctgcagat cctcccagtc actggtccac      540

agcaacggca acacctatct gcattggtac ctccagaagc cgggacaatc ccccaagctc      600

ctgatctaca aggtgtccaa ccggttcagc ggagtgccgg atcgattctc agggtcgggt      660

tcgggaaccg acttcaccct taagatttcc cgggtggaag ccgaggatct cggagtgtac      720

ttctgctccc aaaataccca cgtgccgcct acattcggat cgggaactaa gctggagatc      780

aaggagccca aaagctgcga caagacccac acttgcccac cttgtccgga tccgaagggg      840

ggaactgtgc tcctcctgct gttcgtgatt tcgatcacga ccatcattgt gatcttcctg      900

aaccgccggc gaagacgcga gcggcgcgac ctgttcactg aatcctggga cacccagaag      960

gcccccaaca actacaggtc ccctatctca acctcccaac ccaccaacca gagcatggac     1020

gatactcgcg aggacatcta cgtgaactac cccactttct cccggcggcc taagacccgc     1080

gtgaagcgcg gtcggaagaa gctgctgtac atcttcaagc agcccttcat gcggcctgtg     1140

cagaccacac aggaagagga tggctgctcc tgccgcttcc cggaggaaga ggagggcgga     1200

tgcgaactgc gcgtgaagtt cagccggagc gctgatgccc ctgcatacca gcaggggcag     1260

aaccagctct acaacgaact gaaccttgga cggcgggagg aatacgatgt gctggataag     1320

cgaagaggcc gcgacccaga aatgggcggg aagcccagac gcaagaatcc tcaggaggga     1380

ctgtacaacg agctccagaa agacaagatg gccgaagcgt acagcgagat cggcatgaag     1440

ggggaacgga gaaggggaaa gggccatgac ggattgtacc agggcctgtc gaccgctacc     1500

aaagacacct acgacgccct ccatatgcaa gcactgccgc ctagatgaac gcgt           1554


<210>  115
<211>  1101
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  (nucleotide) hIgG heavy chain signal sequence/scFV 3-17I/hIgG1 
       hinge/CD226 TMD/CD226 ICD v1

<400>  115
atggactgga tctggagaat tttgttcctt gtcggtgctg ccactggagc ccactcgcaa       60

gtgcagctcg tgcagtctgg agcagaagtc aagaagcctg ggtcctcggt caaagtgtcc      120

tgcaaagcct ccgggggcac tttcagctcg tacgcaatct cctgggtccg ccaagcgccc      180

ggacagggtc tggagtggat gggcgggatt atccccattt tcggcaccgc caactatgct      240

caaaagttcc agggccgcgt gactattacc gcggacgagt caacctccac tgcgtacatg      300

gaactgagct cacttcggtc cgaggacacc gccgtctatt actgtgcgcg gggactcctg      360

tggaactact ggggacaagg caccctcgtg accgtgtcct cgaagctgtc cgggtcggcc      420

agcgccccca agttggaaga gggcgaattc tccgaggccc gggtggaaat cgtgatgacc      480

cagtccccgg ccactctctc cgtgtccccg ggagaaaggg cgaccctgtc atgccgggcc      540

tcgcaatccg tgtcatccaa cctggcctgg taccagcaga agccaggaca ggcgccccgc      600

ctgattatct acggcgccag caccaccgcg agcggaattc cggcccggtt ttccgcctcc      660

ggttcgggaa ctgacttcac gctgactatc agcagcctgc aatcggagga cttcgccgtc      720

tactactgcc agcagtataa caactggccc ccggcttaca cgtttggcca gggaactaag      780

ctggagatca aggagcccaa aagctgcgac aagacccaca cttgcccacc ttgtccggac      840

ccgaaggggg gaaccgtgct cctgctgctg ttcgtgatct ccatcaccac aatcatcgtg      900

atcttcctga accgccggcg aagacgcgaa agacgcgatc tgttcaccga gtcatgggac      960

acccagaagg cccctaacaa ctacagaagc ccgatcagca ccagccagcc tactaatcag     1020

tcgatggatg atacccgcga ggacatctac gtgaattacc caaccttctc ccggcggccg     1080

aaaacccgcg tgtgaacgcg t                                               1101


<210>  116
<211>  1254
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  (nucleotide) hIgG heavy chain signal sequence/scFV 3-17I/hIgG1 
       hinge/CD226 TMD/ CD3zeta

<400>  116
atggactgga tctggagaat tttgttcctt gtcggtgctg ccactggagc ccactcgcaa       60

gtgcagctcg tgcagtctgg agcagaagtc aagaagcctg ggtcctcggt caaagtgtcc      120

tgcaaagcct ccgggggcac tttcagctcg tacgcaatct cctgggtccg ccaagcgccc      180

ggacagggtc tggagtggat gggcgggatt atccccattt tcggcaccgc caactatgct      240

caaaagttcc agggccgcgt gactattacc gcggacgagt caacctccac tgcgtacatg      300

gaactgagct cacttcggtc cgaggacacc gccgtctatt actgtgcgcg gggactcctg      360

tggaactact ggggacaagg caccctcgtg accgtgtcct cgaagctgtc cgggtcggcc      420

agcgccccca agttggaaga gggcgaattc tccgaggccc gggtggaaat cgtgatgacc      480

cagtccccgg ccactctctc cgtgtccccg ggagaaaggg cgaccctgtc atgccgggcc      540

tcgcaatccg tgtcatccaa cctggcctgg taccagcaga agccaggaca ggcgccccgc      600

ctgattatct acggcgccag caccaccgcg agcggaattc cggcccggtt ttccgcctcc      660

ggttcgggaa ctgacttcac gctgactatc agcagcctgc aatcggagga cttcgccgtc      720

tactactgcc agcagtataa caactggccc ccggcttaca cgtttggcca gggaactaag      780

ctggagatca aggagcccaa aagctgcgac aagacccaca cttgcccacc ttgtccggac      840

ccgaaggggg gaaccgtgct cctgctgctg ttcgtgatct ccatcaccac aatcatcgtg      900

atcttcctgc gcgtgaagtt cagccggagc gctgatgccc ctgcatacca gcaggggcag      960

aaccagctct acaacgaact gaaccttgga cggcgggagg aatacgatgt gctggataag     1020

cgaagaggcc gcgacccaga aatgggcggg aagcccagac gcaagaatcc tcaggaggga     1080

ctgtacaacg agctccagaa agacaagatg gccgaagcgt acagcgagat cggcatgaag     1140

ggggaacgga gaaggggaaa gggccatgac ggattgtacc agggcctgtc gaccgctacc     1200

aaagacacct acgacgccct ccatatgcaa gcactgccgc cacgctgaac gcgt           1254


<210>  117
<211>  1437
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  (nucleotide) hIgG heavy chain signal sequence/scFV 3-17I/hIgG1 
       hinge/CD226 TMD/CD226 ICD v1/CD3zeta

<400>  117
atggactgga tctggagaat tttgttcctt gtcggtgctg ccactggagc ccactcgcaa       60

gtgcagctcg tgcagtctgg agcagaagtc aagaagcctg ggtcctcggt caaagtgtcc      120

tgcaaagcct ccgggggcac tttcagctcg tacgcaatct cctgggtccg ccaagcgccc      180

ggacagggtc tggagtggat gggcgggatt atccccattt tcggcaccgc caactatgct      240

caaaagttcc agggccgcgt gactattacc gcggacgagt caacctccac tgcgtacatg      300

gaactgagct cacttcggtc cgaggacacc gccgtctatt actgtgcgcg gggactcctg      360

tggaactact ggggacaagg caccctcgtg accgtgtcct cgaagctgtc cgggtcggcc      420

agcgccccca agttggaaga gggcgaattc tccgaggccc gggtggaaat cgtgatgacc      480

cagtccccgg ccactctctc cgtgtccccg ggagaaaggg cgaccctgtc atgccgggcc      540

tcgcaatccg tgtcatccaa cctggcctgg taccagcaga agccaggaca ggcgccccgc      600

ctgattatct acggcgccag caccaccgcg agcggaattc cggcccggtt ttccgcctcc      660

ggttcgggaa ctgacttcac gctgactatc agcagcctgc aatcggagga cttcgccgtc      720

tactactgcc agcagtataa caactggccc ccggcttaca cgtttggcca gggaactaag      780

ctggagatca aggagcccaa aagctgcgac aagacccaca cttgcccacc ttgtccggac      840

ccgaaggggg gaaccgtgct cctgctgctg ttcgtgatct ccatcaccac aatcatcgtg      900

atcttcctga accgccggcg aagacgcgaa agacgcgatc tgttcaccga gtcatgggac      960

acccagaagg cccctaacaa ctacagaagc ccgatcagca ccagccagcc tactaatcag     1020

tcgatggatg atacccgcga ggacatctac gtgaattacc caaccttctc ccggcggccg     1080

aaaacccgcg tgcgcgtgaa gttcagccgg agcgctgatg cccctgcata ccagcagggg     1140

cagaaccagc tctacaacga actgaacctt ggacggcggg aggaatacga tgtgctggat     1200

aagcgaagag gccgcgaccc agaaatgggc gggaagccca gacgcaagaa tcctcaggag     1260

ggactgtaca acgagctcca gaaagacaag atggccgaag cgtacagcga gatcggcatg     1320

aagggggaac ggagaagggg aaagggccat gacggattgt accagggcct gtcgaccgct     1380

accaaagaca cctacgacgc cctccatatg caagcactgc cgccacgctg aacgcgt        1437


<210>  118
<211>  518
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  hIgG heavy chain signal sequence/scFV GC33/hIgG1 hinge/CD28 
       TMD/41BB/CD3zeta /CD226 ICD v1

<400>  118

Met Asp Trp Ile Trp Arg Ile Leu Phe Leu Val Gly Ala Ala Thr Gly 
1               5                   10                  15      


Ala His Ser Gln Val Gln Leu Gln Gln Ser Gly Ala Glu Leu Val Arg 
            20                  25                  30          


Pro Gly Ala Ser Val Lys Leu Ser Cys Lys Ala Ser Gly Tyr Thr Phe 
        35                  40                  45              


Thr Asp Tyr Glu Met His Trp Val Lys Gln Thr Pro Val His Gly Leu 
    50                  55                  60                  


Lys Trp Ile Gly Ala Leu Asp Pro Lys Thr Gly Asp Thr Ala Tyr Ser 
65                  70                  75                  80  


Gln Lys Phe Lys Gly Lys Ala Thr Leu Thr Ala Asp Lys Ser Ser Ser 
                85                  90                  95      


Thr Ala Tyr Met Glu Leu Arg Ser Leu Thr Ser Glu Asp Ser Ala Val 
            100                 105                 110         


Tyr Tyr Cys Thr Arg Phe Tyr Ser Tyr Thr Tyr Trp Gly Gln Gly Thr 
        115                 120                 125             


Leu Val Thr Val Ser Ala Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser 
    130                 135                 140                 


Gly Gly Gly Gly Ser Asp Val Val Met Thr Gln Thr Pro Leu Ser Leu 
145                 150                 155                 160 


Pro Val Ser Leu Gly Asp Gln Ala Ser Ile Ser Cys Arg Ser Ser Gln 
                165                 170                 175     


Ser Leu Val His Ser Asn Gly Asn Thr Tyr Leu His Trp Tyr Leu Gln 
            180                 185                 190         


Lys Pro Gly Gln Ser Pro Lys Leu Leu Ile Tyr Lys Val Ser Asn Arg 
        195                 200                 205             


Phe Ser Gly Val Pro Asp Arg Phe Ser Gly Ser Gly Ser Gly Thr Asp 
    210                 215                 220                 


Phe Thr Leu Lys Ile Ser Arg Val Glu Ala Glu Asp Leu Gly Val Tyr 
225                 230                 235                 240 


Phe Cys Ser Gln Asn Thr His Val Pro Pro Thr Phe Gly Ser Gly Thr 
                245                 250                 255     


Lys Leu Glu Ile Lys Glu Pro Lys Ser Cys Asp Lys Thr His Thr Cys 
            260                 265                 270         


Pro Pro Cys Pro Asp Pro Lys Phe Trp Val Leu Val Val Val Gly Gly 
        275                 280                 285             


Val Leu Ala Cys Tyr Ser Leu Leu Val Thr Val Ala Phe Ile Ile Lys 
    290                 295                 300                 


Arg Gly Arg Lys Lys Leu Leu Tyr Ile Phe Lys Gln Pro Phe Met Arg 
305                 310                 315                 320 


Pro Val Gln Thr Thr Gln Glu Glu Asp Gly Cys Ser Cys Arg Phe Pro 
                325                 330                 335     


Glu Glu Glu Glu Gly Gly Cys Glu Leu Arg Val Lys Phe Ser Arg Ser 
            340                 345                 350         


Ala Asp Ala Pro Ala Tyr Gln Gln Gly Gln Asn Gln Leu Tyr Asn Glu 
        355                 360                 365             


Leu Asn Leu Gly Arg Arg Glu Glu Tyr Asp Val Leu Asp Lys Arg Arg 
    370                 375                 380                 


Gly Arg Asp Pro Glu Met Gly Gly Lys Pro Arg Arg Lys Asn Pro Gln 
385                 390                 395                 400 


Glu Gly Leu Tyr Asn Glu Leu Gln Lys Asp Lys Met Ala Glu Ala Tyr 
                405                 410                 415     


Ser Glu Ile Gly Met Lys Gly Glu Arg Arg Arg Gly Lys Gly His Asp 
            420                 425                 430         


Gly Leu Tyr Gln Gly Leu Ser Thr Ala Thr Lys Asp Thr Tyr Asp Ala 
        435                 440                 445             


Leu His Met Gln Ala Leu Pro Pro Arg Asn Arg Arg Arg Arg Arg Glu 
    450                 455                 460                 


Arg Arg Asp Leu Phe Thr Glu Ser Trp Asp Thr Gln Lys Ala Pro Asn 
465                 470                 475                 480 


Asn Tyr Arg Ser Pro Ile Ser Thr Ser Gln Pro Thr Asn Gln Ser Met 
                485                 490                 495     


Asp Asp Thr Arg Glu Asp Ile Tyr Val Asn Tyr Pro Thr Phe Ser Arg 
            500                 505                 510         


Arg Pro Lys Thr Arg Val 
        515             


<210>  119
<211>  518
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  hIgG heavy chain signal sequence/scFV GC33/hIgG1 hinge/CD28 
       TMD/41BB/CD226 ICD v1/CD3zeta

<400>  119

Met Asp Trp Ile Trp Arg Ile Leu Phe Leu Val Gly Ala Ala Thr Gly 
1               5                   10                  15      


Ala His Ser Gln Val Gln Leu Gln Gln Ser Gly Ala Glu Leu Val Arg 
            20                  25                  30          


Pro Gly Ala Ser Val Lys Leu Ser Cys Lys Ala Ser Gly Tyr Thr Phe 
        35                  40                  45              


Thr Asp Tyr Glu Met His Trp Val Lys Gln Thr Pro Val His Gly Leu 
    50                  55                  60                  


Lys Trp Ile Gly Ala Leu Asp Pro Lys Thr Gly Asp Thr Ala Tyr Ser 
65                  70                  75                  80  


Gln Lys Phe Lys Gly Lys Ala Thr Leu Thr Ala Asp Lys Ser Ser Ser 
                85                  90                  95      


Thr Ala Tyr Met Glu Leu Arg Ser Leu Thr Ser Glu Asp Ser Ala Val 
            100                 105                 110         


Tyr Tyr Cys Thr Arg Phe Tyr Ser Tyr Thr Tyr Trp Gly Gln Gly Thr 
        115                 120                 125             


Leu Val Thr Val Ser Ala Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser 
    130                 135                 140                 


Gly Gly Gly Gly Ser Asp Val Val Met Thr Gln Thr Pro Leu Ser Leu 
145                 150                 155                 160 


Pro Val Ser Leu Gly Asp Gln Ala Ser Ile Ser Cys Arg Ser Ser Gln 
                165                 170                 175     


Ser Leu Val His Ser Asn Gly Asn Thr Tyr Leu His Trp Tyr Leu Gln 
            180                 185                 190         


Lys Pro Gly Gln Ser Pro Lys Leu Leu Ile Tyr Lys Val Ser Asn Arg 
        195                 200                 205             


Phe Ser Gly Val Pro Asp Arg Phe Ser Gly Ser Gly Ser Gly Thr Asp 
    210                 215                 220                 


Phe Thr Leu Lys Ile Ser Arg Val Glu Ala Glu Asp Leu Gly Val Tyr 
225                 230                 235                 240 


Phe Cys Ser Gln Asn Thr His Val Pro Pro Thr Phe Gly Ser Gly Thr 
                245                 250                 255     


Lys Leu Glu Ile Lys Glu Pro Lys Ser Cys Asp Lys Thr His Thr Cys 
            260                 265                 270         


Pro Pro Cys Pro Asp Pro Lys Phe Trp Val Leu Val Val Val Gly Gly 
        275                 280                 285             


Val Leu Ala Cys Tyr Ser Leu Leu Val Thr Val Ala Phe Ile Ile Lys 
    290                 295                 300                 


Arg Gly Arg Lys Lys Leu Leu Tyr Ile Phe Lys Gln Pro Phe Met Arg 
305                 310                 315                 320 


Pro Val Gln Thr Thr Gln Glu Glu Asp Gly Cys Ser Cys Arg Phe Pro 
                325                 330                 335     


Glu Glu Glu Glu Gly Gly Cys Glu Leu Asn Arg Arg Arg Arg Arg Glu 
            340                 345                 350         


Arg Arg Asp Leu Phe Thr Glu Ser Trp Asp Thr Gln Lys Ala Pro Asn 
        355                 360                 365             


Asn Tyr Arg Ser Pro Ile Ser Thr Ser Gln Pro Thr Asn Gln Ser Met 
    370                 375                 380                 


Asp Asp Thr Arg Glu Asp Ile Tyr Val Asn Tyr Pro Thr Phe Ser Arg 
385                 390                 395                 400 


Arg Pro Lys Thr Arg Val Arg Val Lys Phe Ser Arg Ser Ala Asp Ala 
                405                 410                 415     


Pro Ala Tyr Gln Gln Gly Gln Asn Gln Leu Tyr Asn Glu Leu Asn Leu 
            420                 425                 430         


Gly Arg Arg Glu Glu Tyr Asp Val Leu Asp Lys Arg Arg Gly Arg Asp 
        435                 440                 445             


Pro Glu Met Gly Gly Lys Pro Arg Arg Lys Asn Pro Gln Glu Gly Leu 
    450                 455                 460                 


Tyr Asn Glu Leu Gln Lys Asp Lys Met Ala Glu Ala Tyr Ser Glu Ile 
465                 470                 475                 480 


Gly Met Lys Gly Glu Arg Arg Arg Gly Lys Gly His Asp Gly Leu Tyr 
                485                 490                 495     


Gln Gly Leu Ser Thr Ala Thr Lys Asp Thr Tyr Asp Ala Leu His Met 
            500                 505                 510         


Gln Ala Leu Pro Pro Arg 
        515             


<210>  120
<211>  361
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  hIgG heavy chain signal sequence/scFV GC33/hIgG1 hinge/CD226 
       TMD/CD226 ICD v1

<400>  120

Met Asp Trp Ile Trp Arg Ile Leu Phe Leu Val Gly Ala Ala Thr Gly 
1               5                   10                  15      


Ala His Ser Gln Val Gln Leu Gln Gln Ser Gly Ala Glu Leu Val Arg 
            20                  25                  30          


Pro Gly Ala Ser Val Lys Leu Ser Cys Lys Ala Ser Gly Tyr Thr Phe 
        35                  40                  45              


Thr Asp Tyr Glu Met His Trp Val Lys Gln Thr Pro Val His Gly Leu 
    50                  55                  60                  


Lys Trp Ile Gly Ala Leu Asp Pro Lys Thr Gly Asp Thr Ala Tyr Ser 
65                  70                  75                  80  


Gln Lys Phe Lys Gly Lys Ala Thr Leu Thr Ala Asp Lys Ser Ser Ser 
                85                  90                  95      


Thr Ala Tyr Met Glu Leu Arg Ser Leu Thr Ser Glu Asp Ser Ala Val 
            100                 105                 110         


Tyr Tyr Cys Thr Arg Phe Tyr Ser Tyr Thr Tyr Trp Gly Gln Gly Thr 
        115                 120                 125             


Leu Val Thr Val Ser Ala Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser 
    130                 135                 140                 


Gly Gly Gly Gly Ser Asp Val Val Met Thr Gln Thr Pro Leu Ser Leu 
145                 150                 155                 160 


Pro Val Ser Leu Gly Asp Gln Ala Ser Ile Ser Cys Arg Ser Ser Gln 
                165                 170                 175     


Ser Leu Val His Ser Asn Gly Asn Thr Tyr Leu His Trp Tyr Leu Gln 
            180                 185                 190         


Lys Pro Gly Gln Ser Pro Lys Leu Leu Ile Tyr Lys Val Ser Asn Arg 
        195                 200                 205             


Phe Ser Gly Val Pro Asp Arg Phe Ser Gly Ser Gly Ser Gly Thr Asp 
    210                 215                 220                 


Phe Thr Leu Lys Ile Ser Arg Val Glu Ala Glu Asp Leu Gly Val Tyr 
225                 230                 235                 240 


Phe Cys Ser Gln Asn Thr His Val Pro Pro Thr Phe Gly Ser Gly Thr 
                245                 250                 255     


Lys Leu Glu Ile Lys Glu Pro Lys Ser Cys Asp Lys Thr His Thr Cys 
            260                 265                 270         


Pro Pro Cys Pro Asp Pro Lys Gly Gly Thr Val Leu Leu Leu Leu Phe 
        275                 280                 285             


Val Ile Ser Ile Thr Thr Ile Ile Val Ile Phe Leu Asn Arg Arg Arg 
    290                 295                 300                 


Arg Arg Glu Arg Arg Asp Leu Phe Thr Glu Ser Trp Asp Thr Gln Lys 
305                 310                 315                 320 


Ala Pro Asn Asn Tyr Arg Ser Pro Ile Ser Thr Ser Gln Pro Thr Asn 
                325                 330                 335     


Gln Ser Met Asp Asp Thr Arg Glu Asp Ile Tyr Val Asn Tyr Pro Thr 
            340                 345                 350         


Phe Ser Arg Arg Pro Lys Thr Arg Val 
        355                 360     


<210>  121
<211>  499
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  scFV GC33/hIgG1 hinge/CD28 TMD/41BB/CD3zeta /CD226 ICD v1

<400>  121

Gln Val Gln Leu Gln Gln Ser Gly Ala Glu Leu Val Arg Pro Gly Ala 
1               5                   10                  15      


Ser Val Lys Leu Ser Cys Lys Ala Ser Gly Tyr Thr Phe Thr Asp Tyr 
            20                  25                  30          


Glu Met His Trp Val Lys Gln Thr Pro Val His Gly Leu Lys Trp Ile 
        35                  40                  45              


Gly Ala Leu Asp Pro Lys Thr Gly Asp Thr Ala Tyr Ser Gln Lys Phe 
    50                  55                  60                  


Lys Gly Lys Ala Thr Leu Thr Ala Asp Lys Ser Ser Ser Thr Ala Tyr 
65                  70                  75                  80  


Met Glu Leu Arg Ser Leu Thr Ser Glu Asp Ser Ala Val Tyr Tyr Cys 
                85                  90                  95      


Thr Arg Phe Tyr Ser Tyr Thr Tyr Trp Gly Gln Gly Thr Leu Val Thr 
            100                 105                 110         


Val Ser Ala Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly 
        115                 120                 125             


Gly Ser Asp Val Val Met Thr Gln Thr Pro Leu Ser Leu Pro Val Ser 
    130                 135                 140                 


Leu Gly Asp Gln Ala Ser Ile Ser Cys Arg Ser Ser Gln Ser Leu Val 
145                 150                 155                 160 


His Ser Asn Gly Asn Thr Tyr Leu His Trp Tyr Leu Gln Lys Pro Gly 
                165                 170                 175     


Gln Ser Pro Lys Leu Leu Ile Tyr Lys Val Ser Asn Arg Phe Ser Gly 
            180                 185                 190         


Val Pro Asp Arg Phe Ser Gly Ser Gly Ser Gly Thr Asp Phe Thr Leu 
        195                 200                 205             


Lys Ile Ser Arg Val Glu Ala Glu Asp Leu Gly Val Tyr Phe Cys Ser 
    210                 215                 220                 


Gln Asn Thr His Val Pro Pro Thr Phe Gly Ser Gly Thr Lys Leu Glu 
225                 230                 235                 240 


Ile Lys Glu Pro Lys Ser Cys Asp Lys Thr His Thr Cys Pro Pro Cys 
                245                 250                 255     


Pro Asp Pro Lys Phe Trp Val Leu Val Val Val Gly Gly Val Leu Ala 
            260                 265                 270         


Cys Tyr Ser Leu Leu Val Thr Val Ala Phe Ile Ile Lys Arg Gly Arg 
        275                 280                 285             


Lys Lys Leu Leu Tyr Ile Phe Lys Gln Pro Phe Met Arg Pro Val Gln 
    290                 295                 300                 


Thr Thr Gln Glu Glu Asp Gly Cys Ser Cys Arg Phe Pro Glu Glu Glu 
305                 310                 315                 320 


Glu Gly Gly Cys Glu Leu Arg Val Lys Phe Ser Arg Ser Ala Asp Ala 
                325                 330                 335     


Pro Ala Tyr Gln Gln Gly Gln Asn Gln Leu Tyr Asn Glu Leu Asn Leu 
            340                 345                 350         


Gly Arg Arg Glu Glu Tyr Asp Val Leu Asp Lys Arg Arg Gly Arg Asp 
        355                 360                 365             


Pro Glu Met Gly Gly Lys Pro Arg Arg Lys Asn Pro Gln Glu Gly Leu 
    370                 375                 380                 


Tyr Asn Glu Leu Gln Lys Asp Lys Met Ala Glu Ala Tyr Ser Glu Ile 
385                 390                 395                 400 


Gly Met Lys Gly Glu Arg Arg Arg Gly Lys Gly His Asp Gly Leu Tyr 
                405                 410                 415     


Gln Gly Leu Ser Thr Ala Thr Lys Asp Thr Tyr Asp Ala Leu His Met 
            420                 425                 430         


Gln Ala Leu Pro Pro Arg Asn Arg Arg Arg Arg Arg Glu Arg Arg Asp 
        435                 440                 445             


Leu Phe Thr Glu Ser Trp Asp Thr Gln Lys Ala Pro Asn Asn Tyr Arg 
    450                 455                 460                 


Ser Pro Ile Ser Thr Ser Gln Pro Thr Asn Gln Ser Met Asp Asp Thr 
465                 470                 475                 480 


Arg Glu Asp Ile Tyr Val Asn Tyr Pro Thr Phe Ser Arg Arg Pro Lys 
                485                 490                 495     


Thr Arg Val 
            


<210>  122
<211>  499
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  scFV GC33/hIgG1 hinge/CD28 TMD/41BB/CD226 ICD v1/CD3zeta

<400>  122

Gln Val Gln Leu Gln Gln Ser Gly Ala Glu Leu Val Arg Pro Gly Ala 
1               5                   10                  15      


Ser Val Lys Leu Ser Cys Lys Ala Ser Gly Tyr Thr Phe Thr Asp Tyr 
            20                  25                  30          


Glu Met His Trp Val Lys Gln Thr Pro Val His Gly Leu Lys Trp Ile 
        35                  40                  45              


Gly Ala Leu Asp Pro Lys Thr Gly Asp Thr Ala Tyr Ser Gln Lys Phe 
    50                  55                  60                  


Lys Gly Lys Ala Thr Leu Thr Ala Asp Lys Ser Ser Ser Thr Ala Tyr 
65                  70                  75                  80  


Met Glu Leu Arg Ser Leu Thr Ser Glu Asp Ser Ala Val Tyr Tyr Cys 
                85                  90                  95      


Thr Arg Phe Tyr Ser Tyr Thr Tyr Trp Gly Gln Gly Thr Leu Val Thr 
            100                 105                 110         


Val Ser Ala Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly 
        115                 120                 125             


Gly Ser Asp Val Val Met Thr Gln Thr Pro Leu Ser Leu Pro Val Ser 
    130                 135                 140                 


Leu Gly Asp Gln Ala Ser Ile Ser Cys Arg Ser Ser Gln Ser Leu Val 
145                 150                 155                 160 


His Ser Asn Gly Asn Thr Tyr Leu His Trp Tyr Leu Gln Lys Pro Gly 
                165                 170                 175     


Gln Ser Pro Lys Leu Leu Ile Tyr Lys Val Ser Asn Arg Phe Ser Gly 
            180                 185                 190         


Val Pro Asp Arg Phe Ser Gly Ser Gly Ser Gly Thr Asp Phe Thr Leu 
        195                 200                 205             


Lys Ile Ser Arg Val Glu Ala Glu Asp Leu Gly Val Tyr Phe Cys Ser 
    210                 215                 220                 


Gln Asn Thr His Val Pro Pro Thr Phe Gly Ser Gly Thr Lys Leu Glu 
225                 230                 235                 240 


Ile Lys Glu Pro Lys Ser Cys Asp Lys Thr His Thr Cys Pro Pro Cys 
                245                 250                 255     


Pro Asp Pro Lys Phe Trp Val Leu Val Val Val Gly Gly Val Leu Ala 
            260                 265                 270         


Cys Tyr Ser Leu Leu Val Thr Val Ala Phe Ile Ile Lys Arg Gly Arg 
        275                 280                 285             


Lys Lys Leu Leu Tyr Ile Phe Lys Gln Pro Phe Met Arg Pro Val Gln 
    290                 295                 300                 


Thr Thr Gln Glu Glu Asp Gly Cys Ser Cys Arg Phe Pro Glu Glu Glu 
305                 310                 315                 320 


Glu Gly Gly Cys Glu Leu Asn Arg Arg Arg Arg Arg Glu Arg Arg Asp 
                325                 330                 335     


Leu Phe Thr Glu Ser Trp Asp Thr Gln Lys Ala Pro Asn Asn Tyr Arg 
            340                 345                 350         


Ser Pro Ile Ser Thr Ser Gln Pro Thr Asn Gln Ser Met Asp Asp Thr 
        355                 360                 365             


Arg Glu Asp Ile Tyr Val Asn Tyr Pro Thr Phe Ser Arg Arg Pro Lys 
    370                 375                 380                 


Thr Arg Val Arg Val Lys Phe Ser Arg Ser Ala Asp Ala Pro Ala Tyr 
385                 390                 395                 400 


Gln Gln Gly Gln Asn Gln Leu Tyr Asn Glu Leu Asn Leu Gly Arg Arg 
                405                 410                 415     


Glu Glu Tyr Asp Val Leu Asp Lys Arg Arg Gly Arg Asp Pro Glu Met 
            420                 425                 430         


Gly Gly Lys Pro Arg Arg Lys Asn Pro Gln Glu Gly Leu Tyr Asn Glu 
        435                 440                 445             


Leu Gln Lys Asp Lys Met Ala Glu Ala Tyr Ser Glu Ile Gly Met Lys 
    450                 455                 460                 


Gly Glu Arg Arg Arg Gly Lys Gly His Asp Gly Leu Tyr Gln Gly Leu 
465                 470                 475                 480 


Ser Thr Ala Thr Lys Asp Thr Tyr Asp Ala Leu His Met Gln Ala Leu 
                485                 490                 495     


Pro Pro Arg 
            


<210>  123
<211>  342
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  scFV GC33/hIgG1 hinge/CD226 TMD/CD226 ICD v1

<400>  123

Gln Val Gln Leu Gln Gln Ser Gly Ala Glu Leu Val Arg Pro Gly Ala 
1               5                   10                  15      


Ser Val Lys Leu Ser Cys Lys Ala Ser Gly Tyr Thr Phe Thr Asp Tyr 
            20                  25                  30          


Glu Met His Trp Val Lys Gln Thr Pro Val His Gly Leu Lys Trp Ile 
        35                  40                  45              


Gly Ala Leu Asp Pro Lys Thr Gly Asp Thr Ala Tyr Ser Gln Lys Phe 
    50                  55                  60                  


Lys Gly Lys Ala Thr Leu Thr Ala Asp Lys Ser Ser Ser Thr Ala Tyr 
65                  70                  75                  80  


Met Glu Leu Arg Ser Leu Thr Ser Glu Asp Ser Ala Val Tyr Tyr Cys 
                85                  90                  95      


Thr Arg Phe Tyr Ser Tyr Thr Tyr Trp Gly Gln Gly Thr Leu Val Thr 
            100                 105                 110         


Val Ser Ala Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly 
        115                 120                 125             


Gly Ser Asp Val Val Met Thr Gln Thr Pro Leu Ser Leu Pro Val Ser 
    130                 135                 140                 


Leu Gly Asp Gln Ala Ser Ile Ser Cys Arg Ser Ser Gln Ser Leu Val 
145                 150                 155                 160 


His Ser Asn Gly Asn Thr Tyr Leu His Trp Tyr Leu Gln Lys Pro Gly 
                165                 170                 175     


Gln Ser Pro Lys Leu Leu Ile Tyr Lys Val Ser Asn Arg Phe Ser Gly 
            180                 185                 190         


Val Pro Asp Arg Phe Ser Gly Ser Gly Ser Gly Thr Asp Phe Thr Leu 
        195                 200                 205             


Lys Ile Ser Arg Val Glu Ala Glu Asp Leu Gly Val Tyr Phe Cys Ser 
    210                 215                 220                 


Gln Asn Thr His Val Pro Pro Thr Phe Gly Ser Gly Thr Lys Leu Glu 
225                 230                 235                 240 


Ile Lys Glu Pro Lys Ser Cys Asp Lys Thr His Thr Cys Pro Pro Cys 
                245                 250                 255     


Pro Asp Pro Lys Gly Gly Thr Val Leu Leu Leu Leu Phe Val Ile Ser 
            260                 265                 270         


Ile Thr Thr Ile Ile Val Ile Phe Leu Asn Arg Arg Arg Arg Arg Glu 
        275                 280                 285             


Arg Arg Asp Leu Phe Thr Glu Ser Trp Asp Thr Gln Lys Ala Pro Asn 
    290                 295                 300                 


Asn Tyr Arg Ser Pro Ile Ser Thr Ser Gln Pro Thr Asn Gln Ser Met 
305                 310                 315                 320 


Asp Asp Thr Arg Glu Asp Ile Tyr Val Asn Tyr Pro Thr Phe Ser Arg 
                325                 330                 335     


Arg Pro Lys Thr Arg Val 
            340         


<210>  124
<211>  1554
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  (nucleotide) hIgG heavy chain signal sequence/scFV GC33/hIgG1 
       hinge/CD28 TMD/41BB/CD3zeta /CD226 ICD v1

<400>  124
atggattgga tttggcgcat tctgtttctg gtgggcgcgg cgaccggcgc gcatagccag       60

gtgcagctgc agcagagcgg cgcggaactg gtgcgcccgg gcgcgagcgt gaaactgagc      120

tgcaaagcga gcggctatac ctttaccgat tatgaaatgc attgggtgaa acagaccccg      180

gtgcatggcc tgaaatggat tggcgcgctg gatccgaaaa ccggcgatac cgcgtatagc      240

cagaaattta aaggcaaagc gaccctgacc gcggataaaa gcagcagcac cgcgtatatg      300

gaactgcgca gcctgaccag cgaagatagc gcggtgtatt attgcacccg cttttatagc      360

tatacctatt ggggccaggg caccctggtg accgtgagcg cgggcggcgg cggcagcggc      420

ggcggcggca gcggcggcgg cggcagcgat gtggtgatga cccagacccc gctgagcctg      480

ccggtgagcc tgggcgatca ggcgagcatt agctgccgca gcagccagag cctggtgcat      540

agcaacggca acacctatct gcattggtat ctgcagaaac cgggccagag cccgaaactg      600

ctgatttata aagtgagcaa ccgctttagc ggcgtgccgg atcgctttag cggcagcggc      660

agcggcaccg attttaccct gaaaattagc cgcgtggaag cggaagatct gggcgtgtat      720

ttttgcagcc agaacaccca tgtgccgccg acctttggca gcggcaccaa actggaaatt      780

aaagaaccga aaagctgcga taaaacccat acctgcccgc cgtgcccgga tccgaaattt      840

tgggtgctgg tggtggtggg cggcgtgctg gcgtgctata gcctgctggt gaccgtggcg      900

tttattatta aacgcggccg caaaaaactg ctgtatattt ttaaacagcc gtttatgcgc      960

ccggtgcaga ccacccagga agaagatggc tgcagctgcc gctttccgga agaagaagaa     1020

ggcggctgcg aactgcgcgt gaaatttagc cgcagcgcgg atgcgccggc gtatcagcag     1080

ggccagaacc agctgtataa cgaactgaac ctgggccgcc gcgaagaata tgatgtgctg     1140

gataaacgcc gcggccgcga tccggaaatg ggcggcaaac cgcgccgcaa aaacccgcag     1200

gaaggcctgt ataacgaact gcagaaagat aaaatggcgg aagcgtatag cgaaattggc     1260

atgaaaggcg aacgccgccg cggcaaaggc catgatggcc tgtatcaggg cctgagcacc     1320

gcgaccaaag atacctatga tgcgctgcat atgcaggcgc tgccgccgcg caaccgccgc     1380

cgccgccgcg aacgccgcga tctgtttacc gaaagctggg atacccagaa agcgccgaac     1440

aactatcgca gcccgattag caccagccag ccgaccaacc agagcatgga tgatacccgc     1500

gaagatattt atgtgaacta tccgaccttt agccgccgcc cgaaaacccg cgtg           1554


<210>  125
<211>  1554
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  (nucleotide) hIgG heavy chain signal sequence/scFV GC33/hIgG1 
       hinge/CD28 TMD/41BB/CD226 ICD v1/CD3zeta

<400>  125
atggattgga tttggcgcat tctgtttctg gtgggcgcgg cgaccggcgc gcatagccag       60

gtgcagctgc agcagagcgg cgcggaactg gtgcgcccgg gcgcgagcgt gaaactgagc      120

tgcaaagcga gcggctatac ctttaccgat tatgaaatgc attgggtgaa acagaccccg      180

gtgcatggcc tgaaatggat tggcgcgctg gatccgaaaa ccggcgatac cgcgtatagc      240

cagaaattta aaggcaaagc gaccctgacc gcggataaaa gcagcagcac cgcgtatatg      300

gaactgcgca gcctgaccag cgaagatagc gcggtgtatt attgcacccg cttttatagc      360

tatacctatt ggggccaggg caccctggtg accgtgagcg cgggcggcgg cggcagcggc      420

ggcggcggca gcggcggcgg cggcagcgat gtggtgatga cccagacccc gctgagcctg      480

ccggtgagcc tgggcgatca ggcgagcatt agctgccgca gcagccagag cctggtgcat      540

agcaacggca acacctatct gcattggtat ctgcagaaac cgggccagag cccgaaactg      600

ctgatttata aagtgagcaa ccgctttagc ggcgtgccgg atcgctttag cggcagcggc      660

agcggcaccg attttaccct gaaaattagc cgcgtggaag cggaagatct gggcgtgtat      720

ttttgcagcc agaacaccca tgtgccgccg acctttggca gcggcaccaa actggaaatt      780

aaagaaccga aaagctgcga taaaacccat acctgcccgc cgtgcccgga tccgaaattt      840

tgggtgctgg tggtggtggg cggcgtgctg gcgtgctata gcctgctggt gaccgtggcg      900

tttattatta aacgcggccg caaaaaactg ctgtatattt ttaaacagcc gtttatgcgc      960

ccggtgcaga ccacccagga agaagatggc tgcagctgcc gctttccgga agaagaagaa     1020

ggcggctgcg aactgaaccg ccgccgccgc cgcgaacgcc gcgatctgtt taccgaaagc     1080

tgggataccc agaaagcgcc gaacaactat cgcagcccga ttagcaccag ccagccgacc     1140

aaccagagca tggatgatac ccgcgaagat atttatgtga actatccgac ctttagccgc     1200

cgcccgaaaa cccgcgtgcg cgtgaaattt agccgcagcg cggatgcgcc ggcgtatcag     1260

cagggccaga accagctgta taacgaactg aacctgggcc gccgcgaaga atatgatgtg     1320

ctggataaac gccgcggccg cgatccggaa atgggcggca aaccgcgccg caaaaacccg     1380

caggaaggcc tgtataacga actgcagaaa gataaaatgg cggaagcgta tagcgaaatt     1440

ggcatgaaag gcgaacgccg ccgcggcaaa ggccatgatg gcctgtatca gggcctgagc     1500

accgcgacca aagataccta tgatgcgctg catatgcagg cgctgccgcc gcgc           1554


<210>  126
<211>  1083
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  (nucleotide) hIgG heavy chain signal sequence/scFV GC33/hIgG1 
       hinge/CD226 TMD/CD226 ICD v1

<400>  126
atggattgga tttggcgcat tctgtttctg gtgggcgcgg cgaccggcgc gcatagccag       60

gtgcagctgc agcagagcgg cgcggaactg gtgcgcccgg gcgcgagcgt gaaactgagc      120

tgcaaagcga gcggctatac ctttaccgat tatgaaatgc attgggtgaa acagaccccg      180

gtgcatggcc tgaaatggat tggcgcgctg gatccgaaaa ccggcgatac cgcgtatagc      240

cagaaattta aaggcaaagc gaccctgacc gcggataaaa gcagcagcac cgcgtatatg      300

gaactgcgca gcctgaccag cgaagatagc gcggtgtatt attgcacccg cttttatagc      360

tatacctatt ggggccaggg caccctggtg accgtgagcg cgggcggcgg cggcagcggc      420

ggcggcggca gcggcggcgg cggcagcgat gtggtgatga cccagacccc gctgagcctg      480

ccggtgagcc tgggcgatca ggcgagcatt agctgccgca gcagccagag cctggtgcat      540

agcaacggca acacctatct gcattggtat ctgcagaaac cgggccagag cccgaaactg      600

ctgatttata aagtgagcaa ccgctttagc ggcgtgccgg atcgctttag cggcagcggc      660

agcggcaccg attttaccct gaaaattagc cgcgtggaag cggaagatct gggcgtgtat      720

ttttgcagcc agaacaccca tgtgccgccg acctttggca gcggcaccaa actggaaatt      780

aaagaaccga aaagctgcga taaaacccat acctgcccgc cgtgcccgga tccgaaaggc      840

ggcaccgtgc tgctgctgct gtttgtgatt agcattacca ccattattgt gatttttctg      900

aaccgccgcc gccgccgcga acgccgcgat ctgtttaccg aaagctggga tacccagaaa      960

gcgccgaaca actatcgcag cccgattagc accagccagc cgaccaacca gagcatggat     1020

gatacccgcg aagatattta tgtgaactat ccgaccttta gccgccgccc gaaaacccgc     1080

gtg                                                                   1083


