                         SEQUENCE LISTING

<110>  ONCOMED PHARMACEUTICALS, INC.
       GURNEY, AUSTIN L.
       XIE, MING-HONG
 
<120>  PVRIG-BINDING AGENTS AND USES THEREOF

<130>  2293.157PC01/PAC/E-H/M-S

<140>  PCT/US2017/043128
<141>  2017-07-20

<150>  US 62/364,639
<151>  2016-07-20

<160>  33    

<170>  PatentIn version 3.5

<210>  1
<211>  326
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Human PVRIG amino acid sequence

<400>  1

Met Arg Thr Glu Ala Gln Val Pro Ala Leu Gln Pro Pro Glu Pro Gly 
1               5                   10                  15      


Leu Glu Gly Ala Met Gly His Arg Thr Leu Val Leu Pro Trp Val Leu 
            20                  25                  30          


Leu Thr Leu Cys Val Thr Ala Gly Thr Pro Glu Val Trp Val Gln Val 
        35                  40                  45              


Arg Met Glu Ala Thr Glu Leu Ser Ser Phe Thr Ile Arg Cys Gly Phe 
    50                  55                  60                  


Leu Gly Ser Gly Ser Ile Ser Leu Val Thr Val Ser Trp Gly Gly Pro 
65                  70                  75                  80  


Asn Gly Ala Gly Gly Thr Thr Leu Ala Val Leu His Pro Glu Arg Gly 
                85                  90                  95      


Ile Arg Gln Trp Ala Pro Ala Arg Gln Ala Arg Trp Glu Thr Gln Ser 
            100                 105                 110         


Ser Ile Ser Leu Ile Leu Glu Gly Ser Gly Ala Ser Ser Pro Cys Ala 
        115                 120                 125             


Asn Thr Thr Phe Cys Cys Lys Phe Ala Ser Phe Pro Glu Gly Ser Trp 
    130                 135                 140                 


Glu Ala Cys Gly Ser Leu Pro Pro Ser Ser Asp Pro Gly Leu Ser Ala 
145                 150                 155                 160 


Pro Pro Thr Pro Ala Pro Ile Leu Arg Ala Asp Leu Ala Gly Ile Leu 
                165                 170                 175     


Gly Val Ser Gly Val Leu Leu Phe Gly Cys Val Tyr Leu Leu His Leu 
            180                 185                 190         


Leu Arg Arg His Lys His Arg Pro Ala Pro Arg Leu Gln Pro Ser Arg 
        195                 200                 205             


Thr Ser Pro Gln Ala Pro Arg Ala Arg Ala Trp Ala Pro Ser Gln Ala 
    210                 215                 220                 


Ser Gln Ala Ala Leu His Val Pro Tyr Ala Thr Ile Asn Thr Ser Cys 
225                 230                 235                 240 


Arg Pro Ala Thr Leu Asp Thr Ala His Pro His Gly Gly Pro Ser Trp 
                245                 250                 255     


Trp Ala Ser Leu Pro Thr His Ala Ala His Arg Pro Gln Gly Pro Ala 
            260                 265                 270         


Ala Trp Ala Ser Thr Pro Ile Pro Ala Arg Gly Ser Phe Val Ser Val 
        275                 280                 285             


Glu Asn Gly Leu Tyr Ala Gln Ala Gly Glu Arg Pro Pro His Thr Gly 
    290                 295                 300                 


Pro Gly Leu Thr Leu Phe Pro Asp Pro Arg Gly Pro Arg Ala Met Glu 
305                 310                 315                 320 


Gly Pro Leu Gly Val Arg 
                325     


<210>  2
<211>  286
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Human PVRIG amino acid sequence without predicted signal sequence

<400>  2

Thr Pro Glu Val Trp Val Gln Val Arg Met Glu Ala Thr Glu Leu Ser 
1               5                   10                  15      


Ser Phe Thr Ile Arg Cys Gly Phe Leu Gly Ser Gly Ser Ile Ser Leu 
            20                  25                  30          


Val Thr Val Ser Trp Gly Gly Pro Asn Gly Ala Gly Gly Thr Thr Leu 
        35                  40                  45              


Ala Val Leu His Pro Glu Arg Gly Ile Arg Gln Trp Ala Pro Ala Arg 
    50                  55                  60                  


Gln Ala Arg Trp Glu Thr Gln Ser Ser Ile Ser Leu Ile Leu Glu Gly 
65                  70                  75                  80  


Ser Gly Ala Ser Ser Pro Cys Ala Asn Thr Thr Phe Cys Cys Lys Phe 
                85                  90                  95      


Ala Ser Phe Pro Glu Gly Ser Trp Glu Ala Cys Gly Ser Leu Pro Pro 
            100                 105                 110         


Ser Ser Asp Pro Gly Leu Ser Ala Pro Pro Thr Pro Ala Pro Ile Leu 
        115                 120                 125             


Arg Ala Asp Leu Ala Gly Ile Leu Gly Val Ser Gly Val Leu Leu Phe 
    130                 135                 140                 


Gly Cys Val Tyr Leu Leu His Leu Leu Arg Arg His Lys His Arg Pro 
145                 150                 155                 160 


Ala Pro Arg Leu Gln Pro Ser Arg Thr Ser Pro Gln Ala Pro Arg Ala 
                165                 170                 175     


Arg Ala Trp Ala Pro Ser Gln Ala Ser Gln Ala Ala Leu His Val Pro 
            180                 185                 190         


Tyr Ala Thr Ile Asn Thr Ser Cys Arg Pro Ala Thr Leu Asp Thr Ala 
        195                 200                 205             


His Pro His Gly Gly Pro Ser Trp Trp Ala Ser Leu Pro Thr His Ala 
    210                 215                 220                 


Ala His Arg Pro Gln Gly Pro Ala Ala Trp Ala Ser Thr Pro Ile Pro 
225                 230                 235                 240 


Ala Arg Gly Ser Phe Val Ser Val Glu Asn Gly Leu Tyr Ala Gln Ala 
                245                 250                 255     


Gly Glu Arg Pro Pro His Thr Gly Pro Gly Leu Thr Leu Phe Pro Asp 
            260                 265                 270         


Pro Arg Gly Pro Arg Ala Met Glu Gly Pro Leu Gly Val Arg 
        275                 280                 285     


<210>  3
<211>  134
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Human PVRIG predicted extracellular domain amino acid sequence

<400>  3

Thr Pro Glu Val Trp Val Gln Val Arg Met Glu Ala Thr Glu Leu Ser 
1               5                   10                  15      


Ser Phe Thr Ile Arg Cys Gly Phe Leu Gly Ser Gly Ser Ile Ser Leu 
            20                  25                  30          


Val Thr Val Ser Trp Gly Gly Pro Asn Gly Ala Gly Gly Thr Thr Leu 
        35                  40                  45              


Ala Val Leu His Pro Glu Arg Gly Ile Arg Gln Trp Ala Pro Ala Arg 
    50                  55                  60                  


Gln Ala Arg Trp Glu Thr Gln Ser Ser Ile Ser Leu Ile Leu Glu Gly 
65                  70                  75                  80  


Ser Gly Ala Ser Ser Pro Cys Ala Asn Thr Thr Phe Cys Cys Lys Phe 
                85                  90                  95      


Ala Ser Phe Pro Glu Gly Ser Trp Glu Ala Cys Gly Ser Leu Pro Pro 
            100                 105                 110         


Ser Ser Asp Pro Gly Leu Ser Ala Pro Pro Thr Pro Ala Pro Ile Leu 
        115                 120                 125             


Arg Ala Asp Leu Ala Gly 
    130                 


<210>  4
<211>  98
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Human PVRIG predicted IgV-like domain amino acid sequence

<400>  4

Thr Glu Leu Ser Ser Phe Thr Ile Arg Cys Gly Phe Leu Gly Ser Gly 
1               5                   10                  15      


Ser Ile Ser Leu Val Thr Val Ser Trp Gly Gly Pro Asn Gly Ala Gly 
            20                  25                  30          


Gly Thr Thr Leu Ala Val Leu His Pro Glu Arg Gly Ile Arg Gln Trp 
        35                  40                  45              


Ala Pro Ala Arg Gln Ala Arg Trp Glu Thr Gln Ser Ser Ile Ser Leu 
    50                  55                  60                  


Ile Leu Glu Gly Ser Gly Ala Ser Ser Pro Cys Ala Asn Thr Thr Phe 
65                  70                  75                  80  


Cys Cys Lys Phe Ala Ser Phe Pro Glu Gly Ser Trp Glu Ala Cys Gly 
                85                  90                  95      


Ser Leu 
        


<210>  5
<211>  10
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Anti-PVRIG 334MXX Heavy chain CDR1

<400>  5

Gly Tyr Ser Phe Thr Asp Tyr Thr Met Ser 
1               5                   10  


<210>  6
<211>  17
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Anti-PVRIG 334MXX Heavy chain CDR2

<400>  6

Leu Ile Val Pro Tyr Ser Gly Gly Thr Asn Tyr Ser Gln Lys Phe Lys 
1               5                   10                  15      


Gly 
    


<210>  7
<211>  9
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Anti-PVRIG 334MXX Heavy chain CDR3

<400>  7

Glu Gly Tyr Gly Tyr Val Met Asp Tyr 
1               5                   


<210>  8
<211>  11
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Anti-PVRIG 334MXX and anti-TIGIT 313M26 Light chain CDR1

<400>  8

Lys Ala Ser Gln Asp Val Ser Thr Ala Val Ala 
1               5                   10      


<210>  9
<211>  7
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Anti-PVRIG 334MXX and anti-TIGIT 313M26 Light chain CDR2

<400>  9

Ser Ala Ser Tyr Arg Tyr Thr 
1               5           


<210>  10
<211>  7
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Anti-PVRIG 334MXX and anti-TIGIT 313M26 Light chain CDR3

<400>  10

Gln Gln His Tyr Ser Thr Pro 
1               5           


<210>  11
<211>  118
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Anti-PVRIG 334MXX Heavy chain variable region amino acid sequence

<400>  11

Gln Val Gln Leu Gln Gln Ser Gly Pro Glu Leu Val Lys Pro Gly Ala 
1               5                   10                  15      


Ser Val Lys Ile Ser Cys Lys Ala Ser Gly Tyr Ser Phe Thr Asp Tyr 
            20                  25                  30          


Thr Met Ser Trp Val Lys Gln Ser His Gly Lys Asn Leu Glu Trp Ile 
        35                  40                  45              


Gly Leu Ile Val Pro Tyr Ser Gly Gly Thr Asn Tyr Ser Gln Lys Phe 
    50                  55                  60                  


Lys Gly Lys Ala Thr Leu Thr Val Asp Lys Ser Ser Thr Thr Ala Tyr 
65                  70                  75                  80  


Met Glu Phe Phe Ser Leu Thr Ser Glu Asp Ser Ala Val Tyr Tyr Cys 
                85                  90                  95      


Ala Arg Glu Gly Tyr Gly Tyr Val Met Asp Tyr Trp Gly Gln Gly Thr 
            100                 105                 110         


Ser Val Thr Val Ser Ser 
        115             


<210>  12
<211>  108
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Anti-PVRIG 334MXX Light chain variable region amino acid sequence

<400>  12

Asp Ile Val Met Thr Gln Ser His Lys Phe Met Ser Thr Ser Val Gly 
1               5                   10                  15      


Asp Arg Val Ser Ile Thr Cys Lys Ala Ser Gln Asp Val Ser Thr Ala 
            20                  25                  30          


Val Ala Trp Tyr Gln Gln Lys Pro Gly Gln Ser Pro Lys Leu Leu Ile 
        35                  40                  45              


Tyr Ser Ala Ser Tyr Arg Tyr Thr Gly Val Pro Asp Arg Phe Thr Gly 
    50                  55                  60                  


Ser Gly Ser Gly Thr Asp Phe Thr Phe Thr Ile Ser Ser Val Gln Ala 
65                  70                  75                  80  


Glu Asp Leu Ala Val Tyr Tyr Cys Gln Gln His Tyr Ser Thr Pro Trp 
                85                  90                  95      


Thr Phe Gly Gly Gly Thr Lys Leu Glu Ile Lys Arg 
            100                 105             


<210>  13
<211>  354
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Anti-PVRIG 334MXX Heavy chain variable region nucleotide sequence

<400>  13
caggtgcaat tgcaacagtc tggacctgag ctggtgaagc ctggagcttc agtgaagata       60

tcctgcaagg cttctggtta ctcattcact gactacacca tgagctgggt gaagcagagc      120

catggaaaga accttgagtg gattggactt attgttcctt acagtggtgg tactaactac      180

agccagaagt tcaagggcaa ggccacatta actgtagaca agtcctccac cacagcctac      240

atggagtttt tcagtctgac atctgaggac tctgcagtct attactgtgc acgagagggt      300

tacggctatg ttatggacta ctggggtcaa ggaacctcag tcaccgtcag ctca            354


<210>  14
<211>  324
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Anti-PVRIG 334MXX Light chain variable region nucleotide sequence

<400>  14
gatatcgtga tgacccagtc tcacaaattc atgtccacat cagttggaga cagggtcagc       60

atcacctgca aggccagtca ggatgtgagt actgctgtag cctggtatca acagaaacca      120

ggacaatctc ctaaactact gatttactcg gcatcctacc ggtacactgg agtccctgat      180

cgcttcactg gcagtggatc tgggacggat ttcactttca ccatcagcag tgtgcaggct      240

gaagacctgg cagtttatta ctgtcagcaa cattatagta ctccgtggac gttcggtgga      300

ggcaccaagc tggaaatcaa acgt                                             324


<210>  15
<211>  7
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Anti-TIGIT 313M26 Heavy chain CDR1

<400>  15

Thr Ser Asp Tyr Ala Trp Asn 
1               5           


<210>  16
<211>  16
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Anti-TIGIT 313M26 Heavy chain CDR2

<400>  16

Tyr Ile Ser Tyr Ser Gly Ser Thr Ser Tyr Asn Pro Ser Leu Arg Ser 
1               5                   10                  15      


<210>  17
<211>  11
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Anti-TIGIT 313M26 Heavy chain CDR3

<400>  17

Ala Arg Arg Gln Val Gly Leu Gly Phe Ala Tyr 
1               5                   10      


<210>  18
<211>  118
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Anti-TIGIT 313M26 Heavy chain variable region amino acid sequence

<400>  18

Asp Val Gln Leu Gln Glu Ser Gly Pro Gly Leu Val Lys Pro Ser Gln 
1               5                   10                  15      


Ser Leu Ser Leu Thr Cys Thr Val Thr Gly Tyr Ser Ile Thr Ser Asp 
            20                  25                  30          


Tyr Ala Trp Asn Trp Val Arg Gln Phe Pro Gly Asn Lys Leu Glu Trp 
        35                  40                  45              


Met Gly Tyr Ile Ser Tyr Ser Gly Ser Thr Ser Tyr Asn Pro Ser Leu 
    50                  55                  60                  


Arg Ser Arg Ile Ser Ile Thr Arg Asp Thr Ser Lys Asn Gln Phe Phe 
65                  70                  75                  80  


Leu Gln Leu Asn Ser Val Thr Thr Glu Asp Thr Ala Thr Tyr Tyr Cys 
                85                  90                  95      


Ala Arg Arg Gln Val Gly Leu Gly Phe Ala Tyr Trp Gly Gln Gly Thr 
            100                 105                 110         


Leu Val Thr Val Ser Ser 
        115             


<210>  19
<211>  354
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Anti-TIGIT 313M26 Heavy chain variable region nucleotide sequence

<400>  19
gatgtgcagc ttcaggagtc aggacctggc ctggtgaaac cttctcagtc tctgtccctc       60

acctgcactg tcactggcta ctcaatcacc agtgattatg cctggaactg ggtccggcag      120

tttccaggaa acaaactgga gtggatgggc tacataagct acagtggtag cactagctac      180

aacccatctc tcagaagtcg aatctctatc actcgagaca catccaagaa ccagttcttc      240

ctgcagttga attctgtgac tactgaggac acagccacat attactgtgc aaggagacag      300

gtcgggctgg ggtttgctta ctggggccaa gggactctgg tcactgtcag ctca            354


<210>  20
<211>  330
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Human IgG1 Heavy chain constant region

<400>  20

Ala Ser Thr Lys Gly Pro Ser Val Phe Pro Leu Ala Pro Ser Ser Lys 
1               5                   10                  15      


Ser Thr Ser Gly Gly Thr Ala Ala Leu Gly Cys Leu Val Lys Asp Tyr 
            20                  25                  30          


Phe Pro Glu Pro Val Thr Val Ser Trp Asn Ser Gly Ala Leu Thr Ser 
        35                  40                  45              


Gly Val His Thr Phe Pro Ala Val Leu Gln Ser Ser Gly Leu Tyr Ser 
    50                  55                  60                  


Leu Ser Ser Val Val Thr Val Pro Ser Ser Ser Leu Gly Thr Gln Thr 
65                  70                  75                  80  


Tyr Ile Cys Asn Val Asn His Lys Pro Ser Asn Thr Lys Val Asp Lys 
                85                  90                  95      


Lys Val Glu Pro Lys Ser Cys Asp Lys Thr His Thr Cys Pro Pro Cys 
            100                 105                 110         


Pro Ala Pro Glu Leu Leu Gly Gly Pro Ser Val Phe Leu Phe Pro Pro 
        115                 120                 125             


Lys Pro Lys Asp Thr Leu Met Ile Ser Arg Thr Pro Glu Val Thr Cys 
    130                 135                 140                 


Val Val Val Asp Val Ser His Glu Asp Pro Glu Val Lys Phe Asn Trp 
145                 150                 155                 160 


Tyr Val Asp Gly Val Glu Val His Asn Ala Lys Thr Lys Pro Arg Glu 
                165                 170                 175     


Glu Gln Tyr Asn Ser Thr Tyr Arg Val Val Ser Val Leu Thr Val Leu 
            180                 185                 190         


His Gln Asp Trp Leu Asn Gly Lys Glu Tyr Lys Cys Lys Val Ser Asn 
        195                 200                 205             


Lys Ala Leu Pro Ala Pro Ile Glu Lys Thr Ile Ser Lys Ala Lys Gly 
    210                 215                 220                 


Gln Pro Arg Glu Pro Gln Val Tyr Thr Leu Pro Pro Ser Arg Asp Glu 
225                 230                 235                 240 


Leu Thr Lys Asn Gln Val Ser Leu Thr Cys Leu Val Lys Gly Phe Tyr 
                245                 250                 255     


Pro Ser Asp Ile Ala Val Glu Trp Glu Ser Asn Gly Gln Pro Glu Asn 
            260                 265                 270         


Asn Tyr Lys Thr Thr Pro Pro Val Leu Asp Ser Asp Gly Ser Phe Phe 
        275                 280                 285             


Leu Tyr Ser Lys Leu Thr Val Asp Lys Ser Arg Trp Gln Gln Gly Asn 
    290                 295                 300                 


Val Phe Ser Cys Ser Val Met His Glu Ala Leu His Asn His Tyr Thr 
305                 310                 315                 320 


Gln Lys Ser Leu Ser Leu Ser Pro Gly Lys 
                325                 330 


<210>  21
<211>  326
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Human IgG2 Heavy chain constant region

<400>  21

Ala Ser Thr Lys Gly Pro Ser Val Phe Pro Leu Ala Pro Cys Ser Arg 
1               5                   10                  15      


Ser Thr Ser Glu Ser Thr Ala Ala Leu Gly Cys Leu Val Lys Asp Tyr 
            20                  25                  30          


Phe Pro Glu Pro Val Thr Val Ser Trp Asn Ser Gly Ala Leu Thr Ser 
        35                  40                  45              


Gly Val His Thr Phe Pro Ala Val Leu Gln Ser Ser Gly Leu Tyr Ser 
    50                  55                  60                  


Leu Ser Ser Val Val Thr Val Pro Ser Ser Asn Phe Gly Thr Gln Thr 
65                  70                  75                  80  


Tyr Thr Cys Asn Val Asp His Lys Pro Ser Asn Thr Lys Val Asp Lys 
                85                  90                  95      


Thr Val Glu Arg Lys Cys Cys Val Glu Cys Pro Pro Cys Pro Ala Pro 
            100                 105                 110         


Pro Val Ala Gly Pro Ser Val Phe Leu Phe Pro Pro Lys Pro Lys Asp 
        115                 120                 125             


Thr Leu Met Ile Ser Arg Thr Pro Glu Val Thr Cys Val Val Val Asp 
    130                 135                 140                 


Val Ser His Glu Asp Pro Glu Val Gln Phe Asn Trp Tyr Val Asp Gly 
145                 150                 155                 160 


Val Glu Val His Asn Ala Lys Thr Lys Pro Arg Glu Glu Gln Phe Asn 
                165                 170                 175     


Ser Thr Phe Arg Val Val Ser Val Leu Thr Val Val His Gln Asp Trp 
            180                 185                 190         


Leu Asn Gly Lys Glu Tyr Lys Cys Lys Val Ser Asn Lys Gly Leu Pro 
        195                 200                 205             


Ala Pro Ile Glu Lys Thr Ile Ser Lys Thr Lys Gly Gln Pro Arg Glu 
    210                 215                 220                 


Pro Gln Val Tyr Thr Leu Pro Pro Ser Arg Glu Glu Met Thr Lys Asn 
225                 230                 235                 240 


Gln Val Ser Leu Thr Cys Leu Val Lys Gly Phe Tyr Pro Ser Asp Ile 
                245                 250                 255     


Ala Val Glu Trp Glu Ser Asn Gly Gln Pro Glu Asn Asn Tyr Lys Thr 
            260                 265                 270         


Thr Pro Pro Met Leu Asp Ser Asp Gly Ser Phe Phe Leu Tyr Ser Lys 
        275                 280                 285             


Leu Thr Val Asp Lys Ser Arg Trp Gln Gln Gly Asn Val Phe Ser Cys 
    290                 295                 300                 


Ser Val Met His Glu Ala Leu His Asn His Tyr Thr Gln Lys Ser Leu 
305                 310                 315                 320 


Ser Leu Ser Pro Gly Lys 
                325     


<210>  22
<211>  377
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Human IgG3 Heavy chain constant region

<400>  22

Ala Ser Thr Lys Gly Pro Ser Val Phe Pro Leu Ala Pro Cys Ser Arg 
1               5                   10                  15      


Ser Thr Ser Gly Gly Thr Ala Ala Leu Gly Cys Leu Val Lys Asp Tyr 
            20                  25                  30          


Phe Pro Glu Pro Val Thr Val Ser Trp Asn Ser Gly Ala Leu Thr Ser 
        35                  40                  45              


Gly Val His Thr Phe Pro Ala Val Leu Gln Ser Ser Gly Leu Tyr Ser 
    50                  55                  60                  


Leu Ser Ser Val Val Thr Val Pro Ser Ser Ser Leu Gly Thr Gln Thr 
65                  70                  75                  80  


Tyr Thr Cys Asn Val Asn His Lys Pro Ser Asn Thr Lys Val Asp Lys 
                85                  90                  95      


Arg Val Glu Leu Lys Thr Pro Leu Gly Asp Thr Thr His Thr Cys Pro 
            100                 105                 110         


Arg Cys Pro Glu Pro Lys Ser Cys Asp Thr Pro Pro Pro Cys Pro Arg 
        115                 120                 125             


Cys Pro Glu Pro Lys Ser Cys Asp Thr Pro Pro Pro Cys Pro Arg Cys 
    130                 135                 140                 


Pro Glu Pro Lys Ser Cys Asp Thr Pro Pro Pro Cys Pro Arg Cys Pro 
145                 150                 155                 160 


Ala Pro Glu Leu Leu Gly Gly Pro Ser Val Phe Leu Phe Pro Pro Lys 
                165                 170                 175     


Pro Lys Asp Thr Leu Met Ile Ser Arg Thr Pro Glu Val Thr Cys Val 
            180                 185                 190         


Val Val Asp Val Ser His Glu Asp Pro Glu Val Gln Phe Lys Trp Tyr 
        195                 200                 205             


Val Asp Gly Val Glu Val His Asn Ala Lys Thr Lys Pro Arg Glu Glu 
    210                 215                 220                 


Gln Tyr Asn Ser Thr Phe Arg Val Val Ser Val Leu Thr Val Leu His 
225                 230                 235                 240 


Gln Asp Trp Leu Asn Gly Lys Glu Tyr Lys Cys Lys Val Ser Asn Lys 
                245                 250                 255     


Ala Leu Pro Ala Pro Ile Glu Lys Thr Ile Ser Lys Thr Lys Gly Gln 
            260                 265                 270         


Pro Arg Glu Pro Gln Val Tyr Thr Leu Pro Pro Ser Arg Glu Glu Met 
        275                 280                 285             


Thr Lys Asn Gln Val Ser Leu Thr Cys Leu Val Lys Gly Phe Tyr Pro 
    290                 295                 300                 


Ser Asp Ile Ala Val Glu Trp Glu Ser Ser Gly Gln Pro Glu Asn Asn 
305                 310                 315                 320 


Tyr Asn Thr Thr Pro Pro Met Leu Asp Ser Asp Gly Ser Phe Phe Leu 
                325                 330                 335     


Tyr Ser Lys Leu Thr Val Asp Lys Ser Arg Trp Gln Gln Gly Asn Ile 
            340                 345                 350         


Phe Ser Cys Ser Val Met His Glu Ala Leu His Asn Arg Phe Thr Gln 
        355                 360                 365             


Lys Ser Leu Ser Leu Ser Pro Gly Lys 
    370                 375         


<210>  23
<211>  327
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Human IgG4 Heavy chain constant region

<400>  23

Ala Ser Thr Lys Gly Pro Ser Val Phe Pro Leu Ala Pro Cys Ser Arg 
1               5                   10                  15      


Ser Thr Ser Glu Ser Thr Ala Ala Leu Gly Cys Leu Val Lys Asp Tyr 
            20                  25                  30          


Phe Pro Glu Pro Val Thr Val Ser Trp Asn Ser Gly Ala Leu Thr Ser 
        35                  40                  45              


Gly Val His Thr Phe Pro Ala Val Leu Gln Ser Ser Gly Leu Tyr Ser 
    50                  55                  60                  


Leu Ser Ser Val Val Thr Val Pro Ser Ser Ser Leu Gly Thr Lys Thr 
65                  70                  75                  80  


Tyr Thr Cys Asn Val Asp His Lys Pro Ser Asn Thr Lys Val Asp Lys 
                85                  90                  95      


Arg Val Glu Ser Lys Tyr Gly Pro Pro Cys Pro Ser Cys Pro Ala Pro 
            100                 105                 110         


Glu Phe Leu Gly Gly Pro Ser Val Phe Leu Phe Pro Pro Lys Pro Lys 
        115                 120                 125             


Asp Thr Leu Met Ile Ser Arg Thr Pro Glu Val Thr Cys Val Val Val 
    130                 135                 140                 


Asp Val Ser Gln Glu Asp Pro Glu Val Gln Phe Asn Trp Tyr Val Asp 
145                 150                 155                 160 


Gly Val Glu Val His Asn Ala Lys Thr Lys Pro Arg Glu Glu Gln Phe 
                165                 170                 175     


Asn Ser Thr Tyr Arg Val Val Ser Val Leu Thr Val Leu His Gln Asp 
            180                 185                 190         


Trp Leu Asn Gly Lys Glu Tyr Lys Cys Lys Val Ser Asn Lys Gly Leu 
        195                 200                 205             


Pro Ser Ser Ile Glu Lys Thr Ile Ser Lys Ala Lys Gly Gln Pro Arg 
    210                 215                 220                 


Glu Pro Gln Val Tyr Thr Leu Pro Pro Ser Gln Glu Glu Met Thr Lys 
225                 230                 235                 240 


Asn Gln Val Ser Leu Thr Cys Leu Val Lys Gly Phe Tyr Pro Ser Asp 
                245                 250                 255     


Ile Ala Val Glu Trp Glu Ser Asn Gly Gln Pro Glu Asn Asn Tyr Lys 
            260                 265                 270         


Thr Thr Pro Pro Val Leu Asp Ser Asp Gly Ser Phe Phe Leu Tyr Ser 
        275                 280                 285             


Arg Leu Thr Val Asp Lys Ser Arg Trp Gln Glu Gly Asn Val Phe Ser 
    290                 295                 300                 


Cys Ser Val Met His Glu Ala Leu His Asn His Tyr Thr Gln Lys Ser 
305                 310                 315                 320 


Leu Ser Leu Ser Leu Gly Lys 
                325         


<210>  24
<211>  342
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Anti-PVRIG 334M5 Light chain variable region nucleotide sequence

<400>  24
gatatccaga tgacccagtc tccatcctcc ctgagtgtgt cagctggaga gaaggtcact       60

atgagctgca agtccagtca gagtctgtta aacagtgaaa atcaaaagaa ctacttggcc      120

tggttccagc agaaaccagg gcagcctcct aaaatgttga tctacggggc atccactagg      180

gaatctggag tccctgatcg cttcacaggc agtggatctg gaaccgattt cactcttacc      240

atcagcagtg tgcaggctga agacctggca gtttattact gtcagaatga tcatagttat      300

ccattcacgt tcggctcggg gaccaagctg gaaataaaac gt                         342


<210>  25
<211>  114
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Anti-PVRIG 334M5 Light chain variable region amino acid sequence

<400>  25

Asp Ile Gln Met Thr Gln Ser Pro Ser Ser Leu Ser Val Ser Ala Gly 
1               5                   10                  15      


Glu Lys Val Thr Met Ser Cys Lys Ser Ser Gln Ser Leu Leu Asn Ser 
            20                  25                  30          


Glu Asn Gln Lys Asn Tyr Leu Ala Trp Phe Gln Gln Lys Pro Gly Gln 
        35                  40                  45              


Pro Pro Lys Met Leu Ile Tyr Gly Ala Ser Thr Arg Glu Ser Gly Val 
    50                  55                  60                  


Pro Asp Arg Phe Thr Gly Ser Gly Ser Gly Thr Asp Phe Thr Leu Thr 
65                  70                  75                  80  


Ile Ser Ser Val Gln Ala Glu Asp Leu Ala Val Tyr Tyr Cys Gln Asn 
                85                  90                  95      


Asp His Ser Tyr Pro Phe Thr Phe Gly Ser Gly Thr Lys Leu Glu Ile 
            100                 105                 110         


Lys Arg 
        


<210>  26
<211>  17
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Anti-PVRIG 334M5 Light chain CDR1

<400>  26

Lys Ser Ser Gln Ser Leu Leu Asn Ser Glu Asn Gln Lys Asn Tyr Leu 
1               5                   10                  15      


Ala 
    


<210>  27
<211>  7
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Anti-PVRIG 334M5 Light chain CDR2

<400>  27

Gly Ala Ser Thr Arg Glu Ser 
1               5           


<210>  28
<211>  7
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Anti-PVRIG 334M5 Light chain CDR3

<400>  28

Gln Asn Asp His Ser Tyr Pro 
1               5           


<210>  29
<211>  342
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Anti-PVRIG 334M5 Heavy chain variable region nucleotide sequence

<400>  29
gaggtccaat tgcagcagtc tgtggcagag cttgtaaggc ctgggacttc agtgacgata       60

tcctgcaagg cttctggcta caccttcact aactactggc taggttgggt aaaacagagg      120

cctggacatg gacttgagtg gattggagat attttccctg gaggtgatta tcctaactac      180

aatgagaaat tcaagggcaa ggccacactg actgcagaca catcctccag cactgcctac      240

atgcagctca gtagcctgac atctgaggac tcttctgtct atttctgtac aagaggatac      300

ttcgatgtct ggggcgcagg gaccacggtc accgtcagct ca                         342


<210>  30
<211>  114
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Anti-PVRIG 334M5 Heavy chain variable region amino acid sequence

<400>  30

Glu Val Gln Leu Gln Gln Ser Val Ala Glu Leu Val Arg Pro Gly Thr 
1               5                   10                  15      


Ser Val Thr Ile Ser Cys Lys Ala Ser Gly Tyr Thr Phe Thr Asn Tyr 
            20                  25                  30          


Trp Leu Gly Trp Val Lys Gln Arg Pro Gly His Gly Leu Glu Trp Ile 
        35                  40                  45              


Gly Asp Ile Phe Pro Gly Gly Asp Tyr Pro Asn Tyr Asn Glu Lys Phe 
    50                  55                  60                  


Lys Gly Lys Ala Thr Leu Thr Ala Asp Thr Ser Ser Ser Thr Ala Tyr 
65                  70                  75                  80  


Met Gln Leu Ser Ser Leu Thr Ser Glu Asp Ser Ser Val Tyr Phe Cys 
                85                  90                  95      


Thr Arg Gly Tyr Phe Asp Val Trp Gly Ala Gly Thr Thr Val Thr Val 
            100                 105                 110         


Ser Ser 
        


<210>  31
<211>  6
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Anti-PVRIG 334M5 Heavy chain CDR1

<400>  31

Thr Asn Tyr Trp Leu Gly 
1               5       


<210>  32
<211>  17
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Anti-PVRIG 334M5 Heavy chain CDR2

<400>  32

Asp Ile Phe Pro Gly Gly Asp Tyr Pro Asn Tyr Asn Glu Lys Phe Lys 
1               5                   10                  15      


Gly 
    


<210>  33
<211>  7
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Anti-PVRIG 334M5 Heavy chain CDR3

<400>  33

Thr Arg Gly Tyr Phe Asp Val 
1               5           


