                         SEQUENCE LISTING

<110>  Dexter, Jason P Mr.
 
<120>  Biocatalyst Comprising Photoautotrophic Organisms Producing 
       Recombinant Enzymes for Degradation of Toxins Associated With and
        Specific to Harmful Algal Blooms

<130>  US 62/365,301

<160>  7     

<170>  PatentIn version 3.5

<210>  1
<211>  5856
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Plasmid p6803mlrAsec+


<220>
<221>  slr0271 5' homologous recombination site
<222>  (182)..(668)
<223>  From Synechocystis PCC6803 slr0271

<220>
<221>  aphA1 kanamycin resistance gene
<222>  (675)..(1675)

<220>
<221>  cpcB560 promoter
<222>  (1682)..(2241)
<223>  Synechocystis PCC6803 cpcB560 promoter

<220>
<221>  PilA (sll1694) N-terminal secretion tag
<222>  (2242)..(2310)
<223>  Synechocystis PCC6803 PilA (sll1694) secretion tag

<220>
<221>  codon-optimized Sphingopyxis sp. USTB-05 mlrA
<222>  (2311)..(3321)

<220>
<221>  BBa_B0014 terminator
<222>  (3328)..(3422)

<220>
<221>  slr0271 3' homologous recombination site
<222>  (3431)..(3930)
<223>  From Synechocystis PCC 6803

<400>  1
aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccacc tgacgtctaa       60

gaaaccatta ttatcatgac attaacctat aaaaataggc gtatcacgag gccctttcgt      120

tgtaaaacga cggccagtcg aaccacgcaa tgcgtctcga tccgcagtgt cttgcgtctc      180

tccgctgaat ttagtcaaca tcggggacac cctgcacatg tggtttgttt tagcaagggc      240

ttttagaaaa aaatgctcaa tctgaacatc tcccatgaca tttcgcttgg cgatcgccga      300

ggaggaggag gctaaactgt tcgtcttttt ggctttcacc acgtagataa cagtacgagt      360

tattgggtca ttgaattatt aacaatataa ctatttgctg atttttatac ttgcaaattt      420

tagatctaat tgtattagtc ttctatagta gaaatataag gaaatcaaat tttcaaattt      480

tcatcttgaa aaacataaaa tttattcatt atttgttaat cgttgcccca tcaatttgtc      540

gttaaaatgg ttaatagggc tagaagagcg aagtatttgt atttgccgaa aatttcagga      600

gcaaaacaat gcgaatcaac cataagcctc tattaattgc cactgcctta ctaactctcg      660

tgcccaatga gctcacccat gttatattgc acaagataaa aatatatcat catgaacaat      720

aaaactgtct gcttacataa acagtaatac aaggggtgtt atgagccata ttcaacggga      780

aacgtcttgc tcgaggccgc gattaaattc caacctggat gctgatttat atgggtatag      840

atgggctcgc gataatgtcg ggcaatcagg tgcgacaatc tatcgattgt atgggaagcc      900

caatgcgcca gagttgtttc tgaaacatgg caaaggtagc gttgccaatg atgttacaga      960

tgagatggtc agactaaact ggctgacggc atttatgcct cttccgacca tcaagcattt     1020

tatccgtact cctgatgatg catggttact caccactgcg atccccggga aaacagcatt     1080

ccaggtatta gaagaatatc ctgattcagg tgaaaatatt gttgatgcgc tggcagtgtt     1140

cctgcgccgg ttgcattcga ttcctgtttg taattgtcct tttaacagcg atcgcgtatt     1200

tcgtctcgct caggcgcaat cacgaatgaa taacggtttg gttgatgcta gtgattttga     1260

tgacgagcgt aatggctggc ctgttgaaca agtctggaaa gaaatgcata agcttttgcc     1320

attctcaccg gattcagtcg tcactcatgg tgatttctca cttgataacc ttatttttga     1380

cgaggggaaa ttaataggtt gtattgatgt tggacgagtc ggaatcgcag accgatacca     1440

ggatcttgcc atcctatgga actgcctcgg tgagttttct ccttcattac agaaacggct     1500

ttttcaaaaa tatggtattg ataatcctga tatgaataaa ttgcagtttc atttgatgct     1560

cgatgagttt ttctgatagt tagtctttgt agagacacaa acctgaaatt ccggacaccg     1620

cgcttcagga catctccctg gacgccgata tctggcagta tccggacggt actatctgca     1680

gacctgtaga gaagagtccc tgaatatcaa aatggtggga taaaaagctc aaaaaggaaa     1740

gtaggctgtg gttccctagg caacagtctt ccctacccca ctggaaacta aaaaaacgag     1800

aaaagttcgc accgaacatc aattgcataa ttttagccct aaaacataag ctgaacgaaa     1860

ctggttgtct tcccttccca atccaggaca atctgagaat cccctgcaac attacttaac     1920

aaaaaagcag gaataaaatt aacaagatgt aacagacata agtcccatca ccgttgtata     1980

aagttaactg tgggattgca aaagcattca agcctaggcg ctgagctgtt tgagcatccc     2040

ggtggccctt gtcgctgcct ccgtgtttct ccctggattt atttaggtaa tatctctcat     2100

aaatccccgg gtagttaacg aaagttaatg gagatcagta acaataactc tagggtcatt     2160

actttggact ccctcagttt atccggggga attgtgttta agaaaatccc aactcataaa     2220

gtcaagtagg agattaattc catggctagt aattttaaat tcaaactcct ctctcaactc     2280

tccaaaaaac gggcagaagg tggtatgggg atgcgcgaat tcgtgaaaca acggcctctg     2340

ttatgttttt atgctctggc tatcctgatt gccttggccg cccatgccct gcgtgctatg     2400

agccccaccc ctctgggtcc tatgtttaaa atgctgcaag aaactcatgc ccatttgaac     2460

attatcaccg cggttcggag cactttcgaa tatcctggcg cttacaccct gctgctgttt     2520

cctgcggccc ctatgtttgc cgctttaatt gtgaccggca ttggttatgg ccgtgctggt     2580

tttcgcgaac tgttgtcccg ttgtgcccct tggcgtagtc ctgttagctg gcgtcaaggt     2640

gtgaccgtga ttgccgtttg ttttctggct ttttttgcgt tgaccggcat tatgtgggtg     2700

caaacgttta tctatgctcc tcccggcacc ttggatcgta ccttcctgcg ctatggctcc     2760

gaccctctgg ccatttacgc gatgttagcc gcttccttgc tgctgagtcc tggtcctctg     2820

ttagaagaat tgggttggcg cggctttgcc ttacctcaat tgctgaaaaa attcgatcct     2880

ctggctgccg cggtgatttt gggcctgatg tggtgggcct ggcatttacc ccgcgacttg     2940

cctaccctgt tttccggcga acctggcgcg gcgtggggcg tcattgtgaa acaatttgtg     3000

attattcccg gttttattgc tggcaccatt attgctgttt ttgtgtgtaa caaactgggc     3060

ggttccatgt ggggcggtgt gctgattcat gcgatccaca atgaattagg cgtgaatgtt     3120

accgctgaat gggcccccac cgtggccggt ctgggctggc gcccctggga tttagttgaa     3180

tttgccgtgg ccattggtct ggttctgatc tgtggtcgca gcttgggtgc tgccagtcct     3240

gataatgccc gcctggcgtg gggtaatgtt cctcctaaat tacccggtgt tgccaccgat     3300

aaaagtggtg cgaatgcgta gggatcctca cactggctca ccttcgggtg ggcctttctg     3360

cgtttatata ctagagagag aatataaaaa gccagattat taatccggct tttttattat     3420

ttgcggccgc tctttacaat ggccaggtct ttagggagcg gtgaccaacg accaaataat     3480

ttaatttttc caattatttt gatagttatt tggggaacat ggacgaataa aattcggata     3540

aaaagttaaa tttttctaat tctatagtcg gggttttcac ctatgactac tcccgttttt     3600

tgtactaatt gtggcaaccg ccttagcccc caggttcgtt tctgtgaatc ctgtggatgt     3660

cccgttgcct tgacgtcgga gccaccttcc ttttctggac caccattgtc cccattgcct     3720

cctccccctc ccacctttaa tgtcgatgaa gttccatccc ataaaagagg ggtaactcct     3780

tggattcctt ttttcctgtt atttagttct gtcgttgtat tagggggact ttggtggttg     3840

ggagcgttca atctgcccca atggaatcaa tggctagtaa aaattttgcc caccaatacc     3900

agttcccccg ttgttacttc ccctactccg agagacggag tcactgccaa ccgagacggt     3960

catagctgtt tcctgtgtgc cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg     4020

ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttacccac agaatcaggg     4080

gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag     4140

gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga     4200

cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct     4260

ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc     4320

tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg     4380

gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc     4440

tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca     4500

ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag     4560

ttcttgaagt ggtggcctaa ctacggctac actagaagga cagtatttgg tatctgcgct     4620

ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc     4680

accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga     4740

tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca     4800

cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat     4860

taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagttac     4920

caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt     4980

gcctgactcc ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt     5040

gctgcaataa taccgcggga cccacgctca ccggctccag atttatcagc aataaaccag     5100

ccagccggaa gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct     5160

attaattgtt gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt     5220

gttgccatcg ctacaggcat cgtggtatca cgctcgtcgt ttggtatggc ttcattcagc     5280

tccggttccc aacgatcaag gcgagttaca tgatccccca tgttgcgcaa aaaagcggtt     5340

agctccttcg gtcctccgat cgttgtcaga agtaagttgg ccgccgtgtt atcactcatg     5400

gttatggcag cactacataa ttctcttact gtcatgccat ccgtaagatg cttttctgtg     5460

actggtgagt actcaaccaa gtcattctga gaatagtgta tgcggcgacc gagttgctct     5520

tgcccggcgt caatacggga taataccgcg ccacatagca gaactttaaa agtgctcatc     5580

attggaaaac gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt     5640

tcgatgtaac ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt     5700

tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg     5760

aaatgttgaa tactcatact cttccttttt caatattatt gaagcattta tcagggttat     5820

tgtctcatga gcggatacat atttgaatgt atttag                               5856


<210>  2
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  primer 3FW

<400>  2
agggctagaa gagcgaagta                                                   20


<210>  3
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  primer 4REV

<400>  3
acgggagtag tcataggtga a                                                 21


<210>  4
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  primer 9FW

<400>  4
atgcgcgaat tcgtgaaaca ac                                                22


<210>  5
<211>  30
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  primer 10REV

<400>  5
tgaattaatc tcctacttga ctttatgagt                                        30


<210>  6
<211>  6
<212>  PRT
<213>  Sphingopyxis sp. USTB-05


<220>
<221>  METAL
<222>  (1)..(6)

<220>
<221>  misc_feature
<222>  (2)..(3)
<223>  Xaa can be any naturally occurring amino acid

<220>
<221>  misc_feature
<222>  (5)..(5)
<223>  Xaa can be any naturally occurring amino acid

<400>  6

His Xaa Xaa His Xaa Glu 
1               5       


<210>  7
<211>  6
<212>  PRT
<213>  Sphingopyxis sp. USTB-05

<400>  7

His Ala Ile His Asn Glu 
1               5       


