                         SEQUENCE LISTING

<110>  The General Hospital Corporation
 
<120>  Compounds for Targeting Cancer Stem Cells

<130>  29539-0143WO1

<140>  PCT/US2017/042630
<141>  2017-07-18

<150>  US 62/364,215
<151>  2016-07-19

<150>  US 62/483,587
<151>  2017-04-10

<160>  10    

<170>  PatentIn version 3.5

<210>  1
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  qPCR Primer for ChIP-qPCR - ZEB1 (F) (5'-3')

<400>  1
ccagtttgga gagacgttgt aag                                               23


<210>  2
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  qPCR Primer for ChIP-qPCR - ZEB1 (R) (5'-3')

<400>  2
ctctcgccac aggaactgtc                                                   20


<210>  3
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  qPCR Primer for ChIP-qPCR - K4 positive control (F) (5'-3')

<400>  3
cttgattctg agggtcagga g                                                 21


<210>  4
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  qPCR Primer for ChIP-qPCR - K4 positive control (R) (5'-3')

<400>  4
gctgatgcat aggtctggaa g                                                 21


<210>  5
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  qPCR Primer for ChIP-qPCR - Negative control (F) (5'-3')

<400>  5
tggatcttgt gtctgtcact cc                                                22


<210>  6
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  qPCR Primer for ChIP-qPCR - Negative control (R) (5'-3')

<400>  6
aagtggcaag ggagtttagt tg                                                22


<210>  7
<211>  963
<212>  PRT
<213>  HEK-293T cell lysates (human embryotic kidney cells grown in tissue culture)

<400>  7

Met Ala Thr Ala Ala Glu Thr Ser Ala Ser Glu Pro Glu Ala Glu Ser 
1               5                   10                  15      


Lys Ala Gly Pro Lys Ala Asp Gly Glu Glu Asp Glu Val Lys Ala Ala 
            20                  25                  30          


Arg Thr Arg Arg Lys Val Leu Ser Arg Ala Val Ala Ala Ala Thr Tyr 
        35                  40                  45              


Lys Thr Met Gly Pro Ala Trp Asp Gln Gln Glu Glu Gly Val Ser Glu 
    50                  55                  60                  


Ser Asp Gly Asp Glu Tyr Ala Met Ala Ser Ser Ala Glu Ser Ser Pro 
65                  70                  75                  80  


Gly Glu Tyr Glu Trp Glu Tyr Asp Glu Glu Glu Glu Lys Asn Gln Leu 
                85                  90                  95      


Glu Ile Glu Arg Leu Glu Glu Gln Leu Ser Ile Asn Val Tyr Asp Tyr 
            100                 105                 110         


Asn Cys His Val Asp Leu Ile Arg Leu Leu Arg Leu Glu Gly Glu Leu 
        115                 120                 125             


Thr Lys Val Arg Met Ala Arg Gln Lys Met Ser Glu Ile Phe Pro Leu 
    130                 135                 140                 


Thr Glu Glu Leu Trp Leu Glu Trp Leu His Asp Glu Ile Ser Met Ala 
145                 150                 155                 160 


Gln Asp Gly Leu Asp Arg Glu His Val Tyr Asp Leu Phe Glu Lys Ala 
                165                 170                 175     


Val Lys Asp Tyr Ile Cys Pro Asn Ile Trp Leu Glu Tyr Gly Gln Tyr 
            180                 185                 190         


Ser Val Gly Gly Ile Gly Gln Lys Gly Gly Leu Glu Lys Val Arg Ser 
        195                 200                 205             


Val Phe Glu Arg Ala Leu Ser Ser Val Gly Leu His Met Thr Lys Gly 
    210                 215                 220                 


Leu Ala Leu Trp Glu Ala Tyr Arg Glu Phe Glu Ser Ala Ile Val Glu 
225                 230                 235                 240 


Ala Ala Arg Leu Glu Lys Val His Ser Leu Phe Arg Arg Gln Leu Ala 
                245                 250                 255     


Ile Pro Leu Tyr Asp Met Glu Ala Thr Phe Ala Glu Tyr Glu Glu Trp 
            260                 265                 270         


Ser Glu Asp Pro Ile Pro Glu Ser Val Ile Gln Asn Tyr Asn Lys Ala 
        275                 280                 285             


Leu Gln Gln Leu Glu Lys Tyr Lys Pro Tyr Glu Glu Ala Leu Leu Gln 
    290                 295                 300                 


Ala Glu Ala Pro Arg Leu Ala Glu Tyr Gln Ala Tyr Ile Asp Phe Glu 
305                 310                 315                 320 


Met Lys Ile Gly Asp Pro Ala Arg Ile Gln Leu Ile Phe Glu Arg Ala 
                325                 330                 335     


Leu Val Glu Asn Cys Leu Val Pro Asp Leu Trp Ile Arg Tyr Ser Gln 
            340                 345                 350         


Tyr Leu Asp Arg Gln Leu Lys Val Lys Asp Leu Val Leu Ser Val His 
        355                 360                 365             


Asn Arg Ala Ile Arg Asn Cys Pro Trp Thr Val Ala Leu Trp Ser Arg 
    370                 375                 380                 


Tyr Leu Leu Ala Met Glu Arg His Gly Val Asp His Gln Val Ile Ser 
385                 390                 395                 400 


Val Thr Phe Glu Lys Ala Leu Asn Ala Gly Phe Ile Gln Ala Thr Asp 
                405                 410                 415     


Tyr Val Glu Ile Trp Gln Ala Tyr Leu Asp Tyr Leu Arg Arg Arg Val 
            420                 425                 430         


Asp Phe Lys Gln Asp Ser Ser Lys Glu Leu Glu Glu Leu Arg Ala Ala 
        435                 440                 445             


Phe Thr Arg Ala Leu Glu Tyr Leu Lys Gln Glu Val Glu Glu Arg Phe 
    450                 455                 460                 


Asn Glu Ser Gly Asp Pro Ser Cys Val Ile Met Gln Asn Trp Ala Arg 
465                 470                 475                 480 


Ile Glu Ala Arg Leu Cys Asn Asn Met Gln Lys Ala Arg Glu Leu Trp 
                485                 490                 495     


Asp Ser Ile Met Thr Arg Gly Asn Ala Lys Tyr Ala Asn Met Trp Leu 
            500                 505                 510         


Glu Tyr Tyr Asn Leu Glu Arg Ala His Gly Asp Thr Gln His Cys Arg 
        515                 520                 525             


Lys Ala Leu His Arg Ala Val Gln Cys Thr Ser Asp Tyr Pro Glu His 
    530                 535                 540                 


Val Cys Glu Val Leu Leu Thr Met Glu Arg Thr Glu Gly Ser Leu Glu 
545                 550                 555                 560 


Asp Trp Asp Ile Ala Val Gln Lys Thr Glu Thr Arg Leu Ala Arg Val 
                565                 570                 575     


Asn Glu Gln Arg Met Lys Ala Ala Glu Lys Glu Ala Ala Leu Val Gln 
            580                 585                 590         


Gln Glu Glu Glu Lys Ala Glu Gln Arg Lys Arg Ala Arg Ala Glu Lys 
        595                 600                 605             


Lys Ala Leu Lys Lys Lys Lys Lys Ile Arg Gly Pro Glu Lys Arg Gly 
    610                 615                 620                 


Ala Asp Glu Asp Asp Glu Lys Glu Trp Gly Asp Asp Glu Glu Glu Gln 
625                 630                 635                 640 


Pro Ser Lys Arg Arg Arg Val Glu Asn Ser Ile Pro Ala Ala Gly Glu 
                645                 650                 655     


Thr Gln Asn Val Glu Val Ala Ala Gly Pro Ala Gly Lys Cys Ala Ala 
            660                 665                 670         


Val Asp Val Glu Pro Pro Ser Lys Gln Lys Glu Lys Ala Ala Ser Leu 
        675                 680                 685             


Lys Arg Asp Met Pro Lys Val Leu His Asp Ser Ser Lys Asp Ser Ile 
    690                 695                 700                 


Thr Val Phe Val Ser Asn Leu Pro Tyr Ser Met Gln Glu Pro Asp Thr 
705                 710                 715                 720 


Lys Leu Arg Pro Leu Phe Glu Ala Cys Gly Glu Val Val Gln Ile Arg 
                725                 730                 735     


Pro Ile Phe Ser Asn Arg Gly Asp Phe Arg Gly Tyr Cys Tyr Val Glu 
            740                 745                 750         


Phe Lys Glu Glu Lys Ser Ala Leu Gln Ala Leu Glu Met Asp Arg Lys 
        755                 760                 765             


Ser Val Glu Gly Arg Pro Met Phe Val Ser Pro Cys Val Asp Lys Ser 
    770                 775                 780                 


Lys Asn Pro Asp Phe Lys Val Phe Arg Tyr Ser Thr Ser Leu Glu Lys 
785                 790                 795                 800 


His Lys Leu Phe Ile Ser Gly Leu Pro Phe Ser Cys Thr Lys Glu Glu 
                805                 810                 815     


Leu Glu Glu Ile Cys Lys Ala His Gly Thr Val Lys Asp Leu Arg Val 
            820                 825                 830         


Leu Thr Asn Arg Ala Gly Lys Pro Lys Gly Leu Ala Tyr Val Glu Tyr 
        835                 840                 845             


Glu Asn Glu Ser Gln Ala Ser Gln Ala Val Met Lys Met Asp Gly Met 
    850                 855                 860                 


Thr Ile Lys Glu Asn Ile Ile Lys Val Ala Ile Ser Asn Pro Pro Gln 
865                 870                 875                 880 


Arg Lys Val Pro Glu Lys Pro Glu Thr Arg Lys Ala Pro Gly Gly Pro 
                885                 890                 895     


Met Leu Leu Pro Gln Thr Tyr Gly Ala Arg Gly Lys Gly Arg Thr Gln 
            900                 905                 910         


Leu Ser Leu Leu Pro Arg Ala Leu Gln Arg Pro Ser Ala Ala Ala Pro 
        915                 920                 925             


Gln Ala Glu Asn Gly Pro Ala Ala Ala Pro Ala Val Ala Ala Pro Ala 
    930                 935                 940                 


Ala Thr Glu Ala Pro Lys Met Ser Asn Ala Asp Phe Ala Lys Leu Phe 
945                 950                 955                 960 


Leu Arg Lys 
            


<210>  8
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  shRNA for G3BP2, cloned in pLKO_TRC005 lentiviral vector

<400>  8
cgggagtttg tgaggcaata t                                                 21


<210>  9
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  shRNA for G3BP2, cloned in pLKO_TRC005 lentiviral vector

<400>  9
gactctgaca accgtagaat a                                                 21


<210>  10
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  ING4 shRNA insert, cloned in pLKO_TRC005 lentiviral vector

<400>  10
ccgggaaccc acctattgcc t                                                 21


