                         SEQUENCE LISTING

<110>  Kaiima Bio Agritech Ltd
       Cook, Kevin  A.
       Lerenthal, Yaniv
       Rothem, Lilah
 
<120>  COMPOSITIONS AND METHODS FOR GENERATING A HAPLOID OF A TARGET 
       PLANT

<130>  70546

<150>  US 62/364,970
<151>  2016-07-21

<150>  US 62/383,682
<151>  2016-09-06

<160>  79    

<170>  PatentIn version 3.5

<210>  1
<211>  23
<212>  DNA
<213>  Zea mays


<220>
<221>  misc_feature
<223>  gRNA target sequence

<400>  1
accgataagc acagcagctg tgg                                               23


<210>  2
<211>  23
<212>  DNA
<213>  Zea mays


<220>
<221>  misc_feature
<223>  gRNA target sequence

<400>  2
gatgtcttca tcatggatcc agg                                               23


<210>  3
<211>  23
<212>  DNA
<213>  Zea mays


<220>
<221>  misc_feature
<223>  gRNA target sequence

<400>  3
acgagagctg caggcggcca tgg                                               23


<210>  4
<211>  23
<212>  DNA
<213>  Zea mays


<220>
<221>  misc_feature
<223>  gRNA target sequence

<400>  4
cggcgtggcg ccggagctca cgg                                               23


<210>  5
<211>  19
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Single strand DNA oligonucleotide

<400>  5
ctcgtcgctg aggtggatg                                                    19


<210>  6
<211>  20
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Single strand DNA oligonucleotide

<400>  6
gagaagtcga gctgccagaa                                                   20


<210>  7
<211>  20
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Single strand DNA oligonucleotide

<400>  7
aagcagcgta ccttcgacaa                                                   20


<210>  8
<211>  20
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Single strand DNA oligonucleotide

<400>  8
ccgctgatct cgacagagtc                                                   20


<210>  9
<211>  20
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Single strand DNA oligonucleotide

<400>  9
cgcagacggg atcgatctag                                                   20


<210>  10
<211>  20
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Single strand DNA oligonucleotide

<400>  10
aagagaaggg cgccaatcaa                                                   20


<210>  11
<211>  20
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Single strand DNA oligonucleotide

<400>  11
acaagcagaa gaacggcatc                                                   20


<210>  12
<211>  20
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Single strand DNA oligonucleotide

<400>  12
agttgcacgc ttccatcttc                                                   20


<210>  13
<211>  22
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Single strand DNA oligonucleotide

<400>  13
tttgggcctc ctctgatctt tc                                                22


<210>  14
<211>  20
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Single strand DNA oligonucleotide

<400>  14
tttctttggc agcgaaaccg                                                   20


<210>  15
<211>  20
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Single strand DNA oligonucleotide

<400>  15
gtgcttgcca atttcgactc                                                   20


<210>  16
<211>  20
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Single strand DNA oligonucleotide

<400>  16
attcgtggta ctgctgctca                                                   20


<210>  17
<211>  20
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Single strand DNA oligonucleotide

<400>  17
ggagcggtta tgccccaaag                                                   20


<210>  18
<211>  20
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Single strand DNA oligonucleotide

<400>  18
gcaagttcgc gggcaagatc                                                   20


<210>  19
<211>  19
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Single strand DNA oligonucleotide

<400>  19
gccgcgtccc tttgtttga                                                    19


<210>  20
<211>  20
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Single strand DNA oligonucleotide

<400>  20
gggcaaataa tggagggctg                                                   20


<210>  21
<211>  20
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Single strand DNA oligonucleotide

<400>  21
cgcgtggaag agggagaaag                                                   20


<210>  22
<211>  20
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Single strand DNA oligonucleotide

<400>  22
ccaaccagca aatgcacaac                                                   20


<210>  23
<211>  23
<212>  DNA
<213>  Triticum aestivum


<220>
<221>  misc_feature
<223>  gRNA target sequence

<400>  23
agacgtacga gtttgtgcag cgg                                               23


<210>  24
<211>  23
<212>  DNA
<213>  Triticum aestivum


<220>
<221>  misc_feature
<223>  gRNA target sequence

<400>  24
caagacggag atgagcatct ggg                                               23


<210>  25
<211>  19
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Single strand DNA oligonucleotide

<400>  25
cagggactac gacgcggag                                                    19


<210>  26
<211>  19
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Single strand DNA oligonucleotide

<400>  26
ggatggcctc ggcggtttg                                                    19


<210>  27
<211>  27
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Single strand DNA oligonucleotide

<400>  27
ggtgcgattt ctccagcagt aaaaatc                                           27


<210>  28
<211>  26
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Single strand DNA oligonucleotide

<400>  28
ctgagaagat gaagcaccgg cgatat                                            26


<210>  29
<211>  27
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Single strand DNA oligonucleotide

<400>  29
cacatactcg ctactggtca gagaatc                                           27


<210>  30
<211>  22
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Single strand DNA oligonucleotide

<400>  30
ctgaagctga accttcgtct cg                                                22


<210>  31
<211>  20
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Single strand DNA oligonucleotide

<400>  31
aatccagatc ccccgaatta                                                   20


<210>  32
<211>  21
<212>  DNA
<213>  Arabidopsis thaliana

<400>  32
ggactatgtt cttaatcatg g                                                 21


<210>  33
<211>  27
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Single strand DNA oligonucleotide

<400>  33
caccatcgtc aaccactaca tcgagac                                           27


<210>  34
<211>  25
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Single strand DNA oligonucleotide

<400>  34
cagctgccag aaacccacgt catgc                                             25


<210>  35
<211>  18
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Single strand DNA oligonucleotide

<400>  35
atgggcccag aacgacgc                                                     18


<210>  36
<211>  22
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Single strand DNA oligonucleotide

<400>  36
gaagtgagta gcatcgaatg gg                                                22


<210>  37
<211>  29
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Single strand DNA oligonucleotide

<400>  37
catctcagtc tacaactttt cccacatct                                         29


<210>  38
<211>  28
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Single strand DNA oligonucleotide

<400>  38
caaaatagta attcaaggac aggtacat                                          28


<210>  39
<211>  28
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Single strand DNA oligonucleotide

<400>  39
cagtagttat ggataatacc aaccgtct                                          28


<210>  40
<211>  23
<212>  DNA
<213>  Zea mays

<400>  40
gacgaaaata agtcattaac cgg                                               23


<210>  41
<211>  20
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Single strand DNA oligonucleotide

<400>  41
tgggtttgag gatcttgtcc                                                   20


<210>  42
<211>  20
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Single strand DNA oligonucleotide

<400>  42
ctaaaaatcg aggcgaatgc                                                   20


<210>  43
<211>  23
<212>  DNA
<213>  Zea mays


<220>
<221>  misc_feature
<223>  gRNA target sequence

<400>  43
gacgactcta ttacaagaag ggg                                               23


<210>  44
<211>  24
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Single strand DNA oligonucleotide

<400>  44
ccttggaaga gttctctcct tgac                                              24


<210>  45
<211>  24
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Single strand DNA oligonucleotide

<400>  45
gtagtgtctt ggcagatgtc gtag                                              24


<210>  46
<211>  23
<212>  DNA
<213>  Arabidopsis thaliana


<220>
<221>  misc_feature
<223>  gRNA target sequence

<400>  46
gggatccggt gcattagtgc tgg                                               23


<210>  47
<211>  23
<212>  DNA
<213>  Arabidopsis thaliana


<220>
<221>  misc_feature
<223>  gRNA target sequence

<400>  47
gggtttagaa agtacgatta ggg                                               23


<210>  48
<211>  20
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Single strand DNA oligonucleotide

<400>  48
gaattcctcg tgttgcatcc                                                   20


<210>  49
<211>  20
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Single strand DNA oligonucleotide

<400>  49
aaacagaggg atgcaacacg                                                   20


<210>  50
<211>  22
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Single strand DNA oligonucleotide

<400>  50
ccttcacccc cttatattca cc                                                22


<210>  51
<211>  21
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Single strand DNA oligonucleotide

<400>  51
gccacaacta ttgaacgaag c                                                 21


<210>  52
<211>  23
<212>  DNA
<213>  Triticum aestivum


<220>
<221>  misc_feature
<223>  gRNA target sequence

<400>  52
ggcccaataa taaggtgatg tgg                                               23


<210>  53
<211>  23
<212>  DNA
<213>  Triticum aestivum


<220>
<221>  misc_feature
<223>  gRNA target sequence

<400>  53
gcatctcgga gttggaatat agg                                               23


<210>  54
<211>  20
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Single strand DNA oligonucleotide

<400>  54
ttacaagagc tcgtgcatgc                                                   20


<210>  55
<211>  20
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Single strand DNA oligonucleotide

<400>  55
catgggtgca agttatcgac                                                   20


<210>  56
<211>  20
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Single strand DNA oligonucleotide

<400>  56
atgatcggtg agccaatgag                                                   20


<210>  57
<211>  20
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Single strand DNA oligonucleotide

<400>  57
ccagtgtgat gcccaatatg                                                   20


<210>  58
<211>  13314
<212>  DNA
<213>  Artificial sequence

<220>
<223>  CRISPRPL-ADB plasmid backbone

<400>  58
ctcgagcttc tactgggcgg ttttatggac agcaagcgaa ccggaattgc cagctggggc       60

gccctctggt aaggttggga agccctgcaa agtaaactgg atggctttct cgccgccaag      120

gatctgatgg cgcaggggat caagctctga tcaagagaca ggatgaggat cgtttcgcat      180

gattgaacaa gatggattgc acgcaggttc tccggccgct tgggtggaga ggctattcgg      240

ctatgactgg gcacaacaga caatcggctg ctctgatgcc gccgtgttcc ggctgtcagc      300

gcaggggcgc ccggttcttt ttgtcaagac cgacctgtcc ggtgccctga atgaactgca      360

agacgaggca gcgcggctat cgtggctggc cacgacgggc gttccttgcg cagctgtgct      420

cgacgttgtc actgaagcgg gaagggactg gctgctattg ggcgaagtgc cggggcagga      480

tctcctgtca tctcaccttg ctcctgccga gaaagtatcc atcatggctg atgcaatgcg      540

gcggctgcat acgcttgatc cggctacctg cccattcgac caccaagcga aacatcgcat      600

cgagcgagca cgtactcgga tggaagccgg tcttgtcgat caggatgatc tggacgaaga      660

gcatcagggg ctcgcgccag ccgaactgtt cgccaggctc aaggcgagca tgcccgacgg      720

cgaggatctc gtcgtgaccc atggcgatgc ctgcttgccg aatatcatgg tggaaaatgg      780

ccgcttttct ggattcatcg actgtggccg gctgggtgtg gcggaccgct atcaggacat      840

agcgttggct acccgtgata ttgctgaaga gcttggcggc gaatgggctg accgcttcct      900

cgtgctttac ggtatcgccg ctcccgattc gcagcgcatc gccttctatc gccttcttga      960

cgagttcttc tgaattatta acgcttacaa tttcctgatg cggtattttc tccttacgca     1020

tctgtgcggt atttcacacc gcatacaggt ggcacttttc ggggaaatgt gcgcggaacc     1080

cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc     1140

tgataaatgc ttcaataata gcacgtgcta aaacttcatt tttaatttaa aaggatctag     1200

gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac     1260

tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc     1320

gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat     1380

caagagctac caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat     1440

actgtccttc tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct     1500

acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt     1560

cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg     1620

gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta     1680

cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg     1740

gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg     1800

tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc     1860

tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg     1920

ggcttttgct ggccttttgc tcacatgttc ttgactcttc gcgatgtacg ggccagatat     1980

gtcgaccgac atgtcgcaca agtcctaagt tacgcgacag gctgccgccc tgcccttttc     2040

ctggcgtttt cttgtcgcgt gttttagtcg cataaagtag aatacttgcg actagaaccg     2100

gagacattac gccatgaaca agagcgccgc cgctggcctg ctgggctatg cccgcgtcag     2160

caccgacgac caggacttga ccaaccaacg ggccgaactg cacgcggccg gctgcaccaa     2220

gctgttttcc gagaagatca ccggcaccag gcgcgaccgc ccggagctgg ccaggatgct     2280

tgaccaccta cgccctggcg acgttgtgac agtgaccagg ctagaccgcc tggcccgcag     2340

cacccgcgac ctactggaca ttgccgagcg catccaggag gccggcgcgg gcctgcgtag     2400

cctggcagag ccgtgggccg acaccaccac gccggccggc cgcatggtgt tgaccgtgtt     2460

cgccggcatt gccgagttcg agcgttccct aatcatcgac cgcacccgga gcgggcgcga     2520

ggccgccaag gcgcgaggcg tgaagtttgg cccccgccct accctcaccc cggcacagat     2580

cgcgcacgcc cgcgagctga tcgaccagga aggccgcacc gtgaaagagg cggctgcact     2640

gcttggcgtg catcgctcga ccctgtaccg cgcacttgag cgcagcgagg aagtgacgcc     2700

caccgaggcc aggcggcgcg gtgccttccg tgaggacgca ttgaccgagg ccgacgccct     2760

ggcggccgcc gagaatgaac gccaagagga acaagcatga aaccgcacca ggacggccag     2820

gacgaaccgt ttttcattac cgaagagatc gaggcggaga tgatcgcggc cgggtacgtg     2880

ttcgagccgc ccgcgcacgt ctcaaccgtg cggctgcatg aaatcctggc cggtttgtct     2940

gatgccaagc tcgcggcctg gccggcgagc ttggccgctg aagaaaccga gcgccgccgt     3000

ctaaaaaggt gatgtgtatt tgagtaaaac agcttgcgtc atgcggtcgc tgcgtatatg     3060

atgcgatgag taaataaaca aatacgcaag gggaacgcat gaaggttatc gctgtactta     3120

accagaaagg cgggtcaggc aagacgacca tcgcaaccca tctagcccgc gccctgcaac     3180

tcgccggggc cgatgttctg ttagtcgatt ccgatcccca gggcagtgcc cgcgattggg     3240

cggccgtgcg ggaagatcaa ccgctaaccg ttgtcggcat cgaccgcccg acgattgacc     3300

gcgacgtgaa ggccatcggc cggcgcgact tcgtagtgat cgacggagcg ccccaggcgg     3360

cggacttggc tgtgtccgcg atcaaggcag ccgacttcgt gctgattccg gtgcagccaa     3420

gcccttacga catatgggcc accgccgacc tggtggagct ggttaagcag cgcattgagg     3480

tcacggatgg aaggctacaa gcggcctttg tcgtgtcgcg ggcgatcaaa ggcacgcgca     3540

tcggcggtga ggttgccgag gcgctggccg ggtacgagct gcccattctt gagtcccgta     3600

tcacgcagcg cgtgagctac ccaggcactg ccgccgccgg cacaaccgtt cttgaatcag     3660

aacccgaggg cgacgctgcc cgcgaggtcc aggcgctggc cgctgaaatt aaatcaaaac     3720

tcatttgagt taatgaggta aagagaaaat gagcaaaagc acaaacacgc taagtgccgg     3780

ccgtccgagc gcacgcagca gcaaggctgc aacgttggcc agcctggcag acacgccagc     3840

catgaagcgg gtcaactttc agttgccggc ggaggatcac accaagctga agatgtacgc     3900

ggtacgccaa ggcaagacca ttaccgagct gctatctgaa tacatcgcgc agctaccaga     3960

gtaaatgagc aaatgaataa atgagtagat gaattttagc ggctaaagga ggcggcatgg     4020

aaaatcaaga acaaccaggc accgacgccg tggaatgccc catgtgtgga ggaacgggcg     4080

gttggccagg cgtaagcggc tgggttgtct gccggccctg caatggcact ggaaccccca     4140

agcccgagga atcggcgtga gcggtcgcaa accatccggc ccggtacaaa tcggcgcggc     4200

gctgggtgat gacctggtgg agaagttgaa ggcggcgcag gccgcccagc ggcaacgcat     4260

cgaggcagaa gcacgccccg gtgaatcgtg gcaagcggcc gctgatcgaa tccgcaaaga     4320

atcccggcaa ccgccggcag ccggtgcgcc gtcgattagg aagccgccca agggcgacga     4380

gcaaccagat tttttcgttc cgatgctcta tgacgtgggc acccgcgata gtcgcagcat     4440

catggacgtg gccgttttcc gtctgtcgaa gcgtgaccga cgagctggcg aggtgatccg     4500

ctacgagctt ccagacgggc acgtagaggt ttccgcaggg ccggccggca tggcgagtgt     4560

gtgggattac gacctggtac tgatggcggt ttcccatcta accgaatcca tgaaccgata     4620

ccgggaaggg aagggagaca agcccggccg cgtgttccgt ccacacgttg cggacgtact     4680

caagttctgc cggcgagccg atggcggaaa gcagaaagac gacctggtag aaacctgcat     4740

tcggttaaac accacgcacg ttgccatgca gcgtacgaag aaggccaaga acggccgcct     4800

ggtgacggta tccgagggtg aagccttgat tagccgctac aagatcgtaa agagcgaaac     4860

cgggcggccg gagtacatcg agatcgagtt agctgattgg atgtaccgcg agatcacaga     4920

aggcaagaac ccggacgtgc tgacggttca ccccgattac tttttgatcg atcccggcat     4980

cggccgtttt ctctaccgcc tggcacgccg cgccgcaggc aaggcagaag ccagatggtt     5040

gttcaagacg atctacgaac gcagtggcag cgccggagag ttcaagaagt tctgtttcac     5100

cgtgcgcaag ctgatcgggt caaatgacct gccggagtac gatttgaagg aggaggcggg     5160

gcaggctggc ccgatcctag tcatgcgcta ccgcaacctg atcgagggcg aagcatccgc     5220

cggttcctaa tgtacggagc agatgctagg gcaaattgcc ctagcagggg aaaaaggtcg     5280

aaaaggtctc tttcctgtgg atagcacgta cattgggaac ccaaagccgt acattgggaa     5340

ccggaacccg tacattggga acccaaagcc gtacattggg aaccggtcac acatgtaagt     5400

gactgatata aaagagaaaa aaggcgattt ttccgcctaa aactctttaa aacttattaa     5460

aactcttaaa acccgcctgg cctgtgcata actgtctggc cagcgcacag ccgaagagct     5520

gcaaaaagcg cctacccttc ggtcgctgcg ctccctacgc cccgccgctt cgcgtcggcc     5580

tatcgcggcc gctggccgct caaaaatggc tggcctacgg ccaggcaatc taccagggcg     5640

cggacaagcc gcgccgtcgc cactcgaccg ccggcgccca catcaaggca cctctagatg     5700

gcaggatata ttgtggtgta aacagtttaa acagtgtttt actcctcata ttaacttcgg     5760

tcattagagg ccacgatttg acacattttt actcaaaaca aaatgtttgc atatctctta     5820

taatttcaaa ttcaacacac aacaaataag agaaaaaaca aataatatta atttgagaat     5880

gaacaaaagg accatatcat tcattaactc ttctccatcc atttccattt cacagttcga     5940

tagcgaaaac cgaataaaaa acacagtaaa ttacaagcac aacaaatggt acaagaaaaa     6000

cagttttccc aatgccataa tactcgaacg cgtgatctta tcagatttcg gtgacgggca     6060

ggaccggacg gggcggcacc ggcaggctga agtccagctg ccagaaaccc acgtcatgcc     6120

agttcccgtg cttgaagccg gccgcccgca gcatgccgcg gggggcatat ccgagcgcct     6180

cgtgcatgcg cacgctcggg tcgttgggca gcccgatgac agcgaccacg ctcttgaagc     6240

cctgtgcctc cagggacttc aggaggtggg tgtagagcgt ggagcccagt cccgtccgct     6300

ggtggcgggg ggagacgtac acggtggact cggccgtcca gtcgtaggcg ttgcgtgcct     6360

gcacatcaac aaattttggt catatattag aaaagttata aattaaaata tacacactta     6420

taaactacag aaaagcaatt gctatatact acattctttt attttgaaaa aaatatttga     6480

aatattatat tactactaat taatgataat tattatatat atatcaaagg tagaagcaga     6540

aacttacctt ccaggggccc gcgtaggcga tgccggcgac ctcgccgtcc acctcggcga     6600

cgagccaggg atagcgctcc cgcagacgga cgaggtcgtc cgtccactcc tgcggttcct     6660

gcggctcggt acggaagttg accgtgcttg tctcgatgta gtggttgacg atggtgcaga     6720

ccgccggcat gtccgcctcg gtggcacggc ggatgtcggc cgggcgtcgt tctgggccca     6780

ttgttggtac ccagcttggg tctagtcgta ttaagagata gatttgtaga gagagactgg     6840

tgatttcagc gtgtcctctc caaatgaaat gaacttcctt atatagagga aggtcttgcg     6900

aaggatagtg ggattgtgcg tcatccctta cgtcagtgga gatatcacat caatccactt     6960

gctttgaaga cgtggttgga acgtcttctt tttccacgat gctcctcgtg ggtgggggtc     7020

catctttggg accactgtcg gcagaggcat cttgaacgat agcctttcct ttatcgcaat     7080

gatggcattt gtaggtgcca ccttcctttt ctactgtcct tttgatgaag tgacagatag     7140

ctgggcaatg gaatccgagg aggtttcccg atattaccct ttgttgaaaa gtctcaatag     7200

ccctttggtc ttctgagact gtatctttga tattcttgga gtagacgaga gtgtcgtgct     7260

ccaccatgtt atcacatcaa tccacttgct ttgaagacgt ggttggaacg tcttcttttt     7320

ccacgatgct cctcgtgggt gggggtccat ctttgggacc actgtcggca gaggcatctt     7380

gaacgatagc ctttccttta tcgcaatgat ggcatttgta ggtgccacct tccttttcta     7440

ctgtcctttt gatgaagtga cagatagctg ggcaatggaa tccgaggagg tttcccgata     7500

ttaccctttg ttgaaaagtc tcaatagccc tttggtcttc tgagactgta tctttgatat     7560

tcttggagta gacgagagtg tcgtgctcca ccattacata ggcccatcgg agctaacgca     7620

gtgaattcag aaatctcaaa attccggcag aacaattttg aatctcgatc cgtagaaacg     7680

agacggtcat tgttttagtt ccaccacgat tatatttgaa atttacgtga gtgtgagtga     7740

gacttgcata agaaaataaa atctttagtt gggaaaaaat tcaataatat aaatgggctt     7800

gagaaggaag cgagggatag gcctttttct aaaataggcc catttaagct attaacaatc     7860

ttcaaaagta ccacagcgct taggtaaaga aagcagctga gtttatatat ggttagagac     7920

gaagtagtga ttggatggca ggtggaagaa tggacacctg cgagagtttt agagctagaa     7980

atagcaagtt aaaataaggc tagtccgtta tcaacttgaa aaagtggcac cgagtcggtg     8040

ctttttttat cacgaaagct tactgcgtta gctccgatgg gcctatgtaa tggtggagca     8100

cgacactctc gtctactcca agaatatcaa agatacagtc tcagaagacc aaagggctat     8160

tgagactttt caacaaaggg taatatcggg aaacctcctc ggattccatt gcccagctat     8220

ctgtcacttc atcaaaagga cagtagaaaa ggaaggtggc acctacaaat gccatcattg     8280

cgataaagga aaggctatcg ttcaagatgc ctctgccgac agtggtccca aagatggacc     8340

cccacccacg aggagcatcg tggaaaaaga agacgttcca accacgtctt caaagcaagt     8400

ggattgatgt gataacatgg tggagcacga cactctcgtc tactccaaga atatcaaaga     8460

tacagtctca gaagaccaaa gggctattga gacttttcaa caaagggtaa tatcgggaaa     8520

cctcctcgga ttccattgcc cagctatctg tcacttcatc aaaaggacag tagaaaagga     8580

aggtggcacc tacaaatgcc atcattgcga taaaggaaag gctatcgttc aagatgcctc     8640

tgccgacagt ggtcccaaag atggaccccc acccacgagg agcatcgtgg aaaaagaaga     8700

cgttccaacc acgtcttcaa agcaagtgga ttgatgtgat atctccactg acgtaaggga     8760

tgacgcacaa tcccactatc cttcgcaaga ccttcctcta tataaggaag ttcatttcat     8820

ttggagagga cacgctgaaa tcaccagtct ctctctacaa atctatctct taatacgact     8880

cactataggg agacccaagc tggctagcaa caatggataa gaagtactct atcggactcg     8940

atatcggaac taactctgtt ggatgggctg tgatcaccga tgagtacaag gtgccatcta     9000

agaagttcaa ggttctcgga aacaccgata ggcactctat caagaaaaac cttatcggtg     9060

ctctcctctt cgattctggt gaaactgctg aggctaccag actcaagaga accgctagaa     9120

gaaggtacac cagaagaaag aacaggatct gctacctcca agagattttc tctaacgaga     9180

tggctaaagt ggatgattca ttcttccaca ggctcgaaga gtcattcctc gtggaagaag     9240

ataagaagca cgagaggcac cctatcttcg gaaacatcgt tgatgaggtg gcataccacg     9300

agaagtaccc tactatctac cacctcagaa agaagctcgt tgattctact gataaggctg     9360

atctcaggct catctacctc gctctcgctc acatgatcaa gttcagagga cacttcctca     9420

tcgagggtga tctcaaccct gataactctg atgtggataa gttgttcatc cagctcgtgc     9480

agacctacaa ccagcttttc gaagagaacc ctatcaacgc ttcaggtgtg gatgctaagg     9540

ctatcctctc tgctaggctc tctaagtcaa gaaggcttga gaacctcatt gctcagctcc     9600

ctggtgagaa gaagaacgga cttttcggaa acttgatcgc tctctctctc ggactcaccc     9660

ctaacttcaa gtctaacttc gatctcgctg aggatgcaaa gctccagctc tcaaaggata     9720

cctacgatga tgatctcgat aacctcctcg ctcagatcgg agatcagtac gctgatttgt     9780

tcctcgctgc taagaacctc tctgatgcta tcctcctcag tgatatcctc agggtgaaca     9840

ccgagatcac caaggctcca ctttctgctt ctatgatcaa gagatacgat gagcaccacc     9900

aggatctcac acttctcaag gctcttgtta gacagcagct cccagagaag tacaaagaaa     9960

tcttcttcga tcagtctaag aacggatacg ctggttacat cgatggtggt gcatctcaag    10020

aagagttcta caagttcatc aagccaatct tggagaagat ggatggaacc gaggaactcc    10080

tcgtgaagct caatagagag gatctcctta ggaagcagag gaccttcgat aacggatcta    10140

tccctcatca gatccacctc ggagagttgc acgctatcct tagaaggcaa gaggatttct    10200

acccattcct caaggataac agagagaaga ttgagaagat cctcaccttc agaatccctt    10260

actacgtggg acctctcgct agaggaaact caagattcgc ttggatgacc agaaagtctg    10320

aggaaaccat caccccttgg aacttcgaag aggtggtgga taagggtgct agtgctcagt    10380

ctttcatcga gaggatgacc aacttcgata agaaccttcc taacgagaag gtgctcccta    10440

agcactcttt gctctacgag tacttcaccg tgtacaacga gttgaccaag gttaagtacg    10500

tgaccgaggg aatgaggaag cctgcttttt tgtcaggtga gcaaaagaag gctatcgttg    10560

atctcttgtt caagaccaac agaaaggtga ccgtgaagca gctcaaagag gattacttca    10620

agaaaatcga gtgcttcgat tcagtggaaa tctctggtgt tgaggatagg ttcaacgcat    10680

ctctcggaac ctaccacgat ctcctcaaga tcattaagga taaggatttc ttggataacg    10740

aggaaaacga ggatatcttg gaggatatcg ttcttaccct caccctcttc gaggatagag    10800

agatgataga agaaaggctc aagacctacg ctcatctctt cgatgataag gtgatgaagc    10860

agttgaagag aagaagatac actggttggg gaaggctctc aagaaagctc attaacggaa    10920

tcagggataa gcagtctgga aagacaatcc ttgatttcct caagtctgat ggattcgcta    10980

acagaaactt catgcagctc atccacgatg attctctcac ctttaaagag gatatccaga    11040

aggctcaggt ttcaggacag ggtgatagtc tccatgagca tatcgctaac ctcgctggat    11100

cccctgcaat caagaaggga atcctccaga ctgtgaagat tgtggatgag ttggtgaagg    11160

tgatgggaca caagcctgag aacatcgtga tcgaaatggc tagagagaac cagaccactc    11220

agaagggaca gaagaactct agggaaagga tgaagaggat cgaggaaggt atcaaagagc    11280

ttggatctca gatcctcaaa gagcaccctg ttgagaacac tcagctccag aacgagaagc    11340

tctacctcta ctacttgcag aacggaaggg atatgtatgt ggatcaagag cttgatatta    11400

acaggctctc tgattacgat gttgatcata tcgtgccaca gtcttttatc aaagatgatt    11460

ctatcgataa caaggtgctc actaggtctg ataagaacag gggtaagagt gataacgtgc    11520

caagtgaaga ggttgtgaag aaaatgaaga actattggag gcagctcctc aacgctaagc    11580

tcatcactca gagaaagttc gataacttga ccaaggctga gaggggagga ctctctgaat    11640

tggataaggc aggattcatc aagagacagc tcgtggaaac caggcagatc accaaacatg    11700

tggcacagat cctcgattct aggatgaaca ccaagtacga tgagaacgat aagttgatca    11760

gggaagtgaa ggttatcacc ctcaagtcaa agctcgtgtc tgatttcaga aaggatttcc    11820

aattctacaa ggtgagggaa atcaacaact accaccacgc tcacgatgct taccttaacg    11880

ctgttgttgg aaccgctctc atcaagaagt atccaaagtt ggagtctgag ttcgtgtacg    11940

gtgattataa ggtgtacgat gtgaggaaga tgatcgctaa gtctgagcaa gagatcggaa    12000

aggctaccgc taagtatttc ttctactcta acatcatgaa tttcttcaag accgagatca    12060

ctctcgctaa cggtgagatc agaaagaggc cactcatcga gacaaacggt gaaacaggtg    12120

agatcgtgtg ggataaggga agggatttcg ctaccgttag aaaggtgctc tctatgcctc    12180

aggtgaacat cgttaagaaa accgaggtgc agaccggtgg attctctaaa gagtctatcc    12240

tccctaagag gaactctgat aagctcattg ctaggaagaa ggattgggac cctaagaaat    12300

acggtggttt cgattctcct accgtggctt actctgttct cgttgtggct aaggttgaga    12360

agggaaagag taagaagctc aagtctgtta aggaacttct cggaatcact atcatggaaa    12420

ggtcatcttt cgagaagaac ccaatcgatt tccttgaggc taagggatac aaagaggtta    12480

agaaggatct catcatcaag ctcccaaagt actcactttt cgagttggag aacggtagaa    12540

agaggatgct cgcttctgct ggtgagcttc aaaagggaaa cgagcttgct ctcccatcta    12600

agtacgttaa ctttctttac ctcgcttctc actacgagaa gttgaaggga tctccagaag    12660

ataacgagca gaagcaactt ttcgttgagc agcacaagca ctacttggat gagatcatcg    12720

agcagatcag tgagttctct aaaagggtga tcctcgctga tgcaaacctc gataaggtgt    12780

tgtctgctta caacaagcac agagataagc ctatcaggga acaggcagag aacatcatcc    12840

atctcttcac ccttaccaac ctcggtgctc ctgctgcttt caagtacttc gatacaacca    12900

tcgataggaa gagatacacc tctaccaaag aagtgctcga tgctaccctc atccatcagt    12960

ctatcactgg actctacgag actaggatcg atctctcaca gcttggaggt gatcctaaga    13020

agaaaagaaa ggttagatct tgatgacccg ggtctccata ataatgtgtg agtagttccc    13080

agataaggga attagggttc ctatagggtt tcgctcatgt gttgagcata taagaaaccc    13140

ttagtatgta tttgtatttg taaaatactt ctatcaataa aatttctaat tcctaaaacc    13200

aaaatccagt actaaaatcc agatcccccg aattaaggcc ttgacaggat atattggcgg    13260

gtaaacctaa gagaaaagag cgtttattag aataacggat atttaaaact cgag          13314


<210>  59
<211>  13300
<212>  DNA
<213>  Artificial sequence

<220>
<223>  CRISPRPL-ADB cloned with GL1-1 gRNA sequence

<400>  59
ctcgagcttc tactgggcgg ttttatggac agcaagcgaa ccggaattgc cagctggggc       60

gccctctggt aaggttggga agccctgcaa agtaaactgg atggctttct cgccgccaag      120

gatctgatgg cgcaggggat caagctctga tcaagagaca ggatgaggat cgtttcgcat      180

gattgaacaa gatggattgc acgcaggttc tccggccgct tgggtggaga ggctattcgg      240

ctatgactgg gcacaacaga caatcggctg ctctgatgcc gccgtgttcc ggctgtcagc      300

gcaggggcgc ccggttcttt ttgtcaagac cgacctgtcc ggtgccctga atgaactgca      360

agacgaggca gcgcggctat cgtggctggc cacgacgggc gttccttgcg cagctgtgct      420

cgacgttgtc actgaagcgg gaagggactg gctgctattg ggcgaagtgc cggggcagga      480

tctcctgtca tctcaccttg ctcctgccga gaaagtatcc atcatggctg atgcaatgcg      540

gcggctgcat acgcttgatc cggctacctg cccattcgac caccaagcga aacatcgcat      600

cgagcgagca cgtactcgga tggaagccgg tcttgtcgat caggatgatc tggacgaaga      660

gcatcagggg ctcgcgccag ccgaactgtt cgccaggctc aaggcgagca tgcccgacgg      720

cgaggatctc gtcgtgaccc atggcgatgc ctgcttgccg aatatcatgg tggaaaatgg      780

ccgcttttct ggattcatcg actgtggccg gctgggtgtg gcggaccgct atcaggacat      840

agcgttggct acccgtgata ttgctgaaga gcttggcggc gaatgggctg accgcttcct      900

cgtgctttac ggtatcgccg ctcccgattc gcagcgcatc gccttctatc gccttcttga      960

cgagttcttc tgaattatta acgcttacaa tttcctgatg cggtattttc tccttacgca     1020

tctgtgcggt atttcacacc gcatacaggt ggcacttttc ggggaaatgt gcgcggaacc     1080

cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc     1140

tgataaatgc ttcaataata gcacgtgcta aaacttcatt tttaatttaa aaggatctag     1200

gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac     1260

tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc     1320

gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat     1380

caagagctac caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat     1440

actgtccttc tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct     1500

acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt     1560

cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg     1620

gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta     1680

cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg     1740

gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg     1800

tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc     1860

tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg     1920

ggcttttgct ggccttttgc tcacatgttc ttgactcttc gcgatgtacg ggccagatat     1980

gtcgaccgac atgtcgcaca agtcctaagt tacgcgacag gctgccgccc tgcccttttc     2040

ctggcgtttt cttgtcgcgt gttttagtcg cataaagtag aatacttgcg actagaaccg     2100

gagacattac gccatgaaca agagcgccgc cgctggcctg ctgggctatg cccgcgtcag     2160

caccgacgac caggacttga ccaaccaacg ggccgaactg cacgcggccg gctgcaccaa     2220

gctgttttcc gagaagatca ccggcaccag gcgcgaccgc ccggagctgg ccaggatgct     2280

tgaccaccta cgccctggcg acgttgtgac agtgaccagg ctagaccgcc tggcccgcag     2340

cacccgcgac ctactggaca ttgccgagcg catccaggag gccggcgcgg gcctgcgtag     2400

cctggcagag ccgtgggccg acaccaccac gccggccggc cgcatggtgt tgaccgtgtt     2460

cgccggcatt gccgagttcg agcgttccct aatcatcgac cgcacccgga gcgggcgcga     2520

ggccgccaag gcgcgaggcg tgaagtttgg cccccgccct accctcaccc cggcacagat     2580

cgcgcacgcc cgcgagctga tcgaccagga aggccgcacc gtgaaagagg cggctgcact     2640

gcttggcgtg catcgctcga ccctgtaccg cgcacttgag cgcagcgagg aagtgacgcc     2700

caccgaggcc aggcggcgcg gtgccttccg tgaggacgca ttgaccgagg ccgacgccct     2760

ggcggccgcc gagaatgaac gccaagagga acaagcatga aaccgcacca ggacggccag     2820

gacgaaccgt ttttcattac cgaagagatc gaggcggaga tgatcgcggc cgggtacgtg     2880

ttcgagccgc ccgcgcacgt ctcaaccgtg cggctgcatg aaatcctggc cggtttgtct     2940

gatgccaagc tcgcggcctg gccggcgagc ttggccgctg aagaaaccga gcgccgccgt     3000

ctaaaaaggt gatgtgtatt tgagtaaaac agcttgcgtc atgcggtcgc tgcgtatatg     3060

atgcgatgag taaataaaca aatacgcaag gggaacgcat gaaggttatc gctgtactta     3120

accagaaagg cgggtcaggc aagacgacca tcgcaaccca tctagcccgc gccctgcaac     3180

tcgccggggc cgatgttctg ttagtcgatt ccgatcccca gggcagtgcc cgcgattggg     3240

cggccgtgcg ggaagatcaa ccgctaaccg ttgtcggcat cgaccgcccg acgattgacc     3300

gcgacgtgaa ggccatcggc cggcgcgact tcgtagtgat cgacggagcg ccccaggcgg     3360

cggacttggc tgtgtccgcg atcaaggcag ccgacttcgt gctgattccg gtgcagccaa     3420

gcccttacga catatgggcc accgccgacc tggtggagct ggttaagcag cgcattgagg     3480

tcacggatgg aaggctacaa gcggcctttg tcgtgtcgcg ggcgatcaaa ggcacgcgca     3540

tcggcggtga ggttgccgag gcgctggccg ggtacgagct gcccattctt gagtcccgta     3600

tcacgcagcg cgtgagctac ccaggcactg ccgccgccgg cacaaccgtt cttgaatcag     3660

aacccgaggg cgacgctgcc cgcgaggtcc aggcgctggc cgctgaaatt aaatcaaaac     3720

tcatttgagt taatgaggta aagagaaaat gagcaaaagc acaaacacgc taagtgccgg     3780

ccgtccgagc gcacgcagca gcaaggctgc aacgttggcc agcctggcag acacgccagc     3840

catgaagcgg gtcaactttc agttgccggc ggaggatcac accaagctga agatgtacgc     3900

ggtacgccaa ggcaagacca ttaccgagct gctatctgaa tacatcgcgc agctaccaga     3960

gtaaatgagc aaatgaataa atgagtagat gaattttagc ggctaaagga ggcggcatgg     4020

aaaatcaaga acaaccaggc accgacgccg tggaatgccc catgtgtgga ggaacgggcg     4080

gttggccagg cgtaagcggc tgggttgtct gccggccctg caatggcact ggaaccccca     4140

agcccgagga atcggcgtga gcggtcgcaa accatccggc ccggtacaaa tcggcgcggc     4200

gctgggtgat gacctggtgg agaagttgaa ggcggcgcag gccgcccagc ggcaacgcat     4260

cgaggcagaa gcacgccccg gtgaatcgtg gcaagcggcc gctgatcgaa tccgcaaaga     4320

atcccggcaa ccgccggcag ccggtgcgcc gtcgattagg aagccgccca agggcgacga     4380

gcaaccagat tttttcgttc cgatgctcta tgacgtgggc acccgcgata gtcgcagcat     4440

catggacgtg gccgttttcc gtctgtcgaa gcgtgaccga cgagctggcg aggtgatccg     4500

ctacgagctt ccagacgggc acgtagaggt ttccgcaggg ccggccggca tggcgagtgt     4560

gtgggattac gacctggtac tgatggcggt ttcccatcta accgaatcca tgaaccgata     4620

ccgggaaggg aagggagaca agcccggccg cgtgttccgt ccacacgttg cggacgtact     4680

caagttctgc cggcgagccg atggcggaaa gcagaaagac gacctggtag aaacctgcat     4740

tcggttaaac accacgcacg ttgccatgca gcgtacgaag aaggccaaga acggccgcct     4800

ggtgacggta tccgagggtg aagccttgat tagccgctac aagatcgtaa agagcgaaac     4860

cgggcggccg gagtacatcg agatcgagtt agctgattgg atgtaccgcg agatcacaga     4920

aggcaagaac ccggacgtgc tgacggttca ccccgattac tttttgatcg atcccggcat     4980

cggccgtttt ctctaccgcc tggcacgccg cgccgcaggc aaggcagaag ccagatggtt     5040

gttcaagacg atctacgaac gcagtggcag cgccggagag ttcaagaagt tctgtttcac     5100

cgtgcgcaag ctgatcgggt caaatgacct gccggagtac gatttgaagg aggaggcggg     5160

gcaggctggc ccgatcctag tcatgcgcta ccgcaacctg atcgagggcg aagcatccgc     5220

cggttcctaa tgtacggagc agatgctagg gcaaattgcc ctagcagggg aaaaaggtcg     5280

aaaaggtctc tttcctgtgg atagcacgta cattgggaac ccaaagccgt acattgggaa     5340

ccggaacccg tacattggga acccaaagcc gtacattggg aaccggtcac acatgtaagt     5400

gactgatata aaagagaaaa aaggcgattt ttccgcctaa aactctttaa aacttattaa     5460

aactcttaaa acccgcctgg cctgtgcata actgtctggc cagcgcacag ccgaagagct     5520

gcaaaaagcg cctacccttc ggtcgctgcg ctccctacgc cccgccgctt cgcgtcggcc     5580

tatcgcggcc gctggccgct caaaaatggc tggcctacgg ccaggcaatc taccagggcg     5640

cggacaagcc gcgccgtcgc cactcgaccg ccggcgccca catcaaggca cctctagatg     5700

gcaggatata ttgtggtgta aacagtttaa acagtgtttt actcctcata ttaacttcgg     5760

tcattagagg ccacgatttg acacattttt actcaaaaca aaatgtttgc atatctctta     5820

taatttcaaa ttcaacacac aacaaataag agaaaaaaca aataatatta atttgagaat     5880

gaacaaaagg accatatcat tcattaactc ttctccatcc atttccattt cacagttcga     5940

tagcgaaaac cgaataaaaa acacagtaaa ttacaagcac aacaaatggt acaagaaaaa     6000

cagttttccc aatgccataa tactcgaacg cgtgatctta tcagatttcg gtgacgggca     6060

ggaccggacg gggcggcacc ggcaggctga agtccagctg ccagaaaccc acgtcatgcc     6120

agttcccgtg cttgaagccg gccgcccgca gcatgccgcg gggggcatat ccgagcgcct     6180

cgtgcatgcg cacgctcggg tcgttgggca gcccgatgac agcgaccacg ctcttgaagc     6240

cctgtgcctc cagggacttc aggaggtggg tgtagagcgt ggagcccagt cccgtccgct     6300

ggtggcgggg ggagacgtac acggtggact cggccgtcca gtcgtaggcg ttgcgtgcct     6360

gcacatcaac aaattttggt catatattag aaaagttata aattaaaata tacacactta     6420

taaactacag aaaagcaatt gctatatact acattctttt attttgaaaa aaatatttga     6480

aatattatat tactactaat taatgataat tattatatat atatcaaagg tagaagcaga     6540

aacttacctt ccaggggccc gcgtaggcga tgccggcgac ctcgccgtcc acctcggcga     6600

cgagccaggg atagcgctcc cgcagacgga cgaggtcgtc cgtccactcc tgcggttcct     6660

gcggctcggt acggaagttg accgtgcttg tctcgatgta gtggttgacg atggtgcaga     6720

ccgccggcat gtccgcctcg gtggcacggc ggatgtcggc cgggcgtcgt tctgggccca     6780

ttgttggtac ccagcttggg tctagtcgta ttaagagata gatttgtaga gagagactgg     6840

tgatttcagc gtgtcctctc caaatgaaat gaacttcctt atatagagga aggtcttgcg     6900

aaggatagtg ggattgtgcg tcatccctta cgtcagtgga gatatcacat caatccactt     6960

gctttgaaga cgtggttgga acgtcttctt tttccacgat gctcctcgtg ggtgggggtc     7020

catctttggg accactgtcg gcagaggcat cttgaacgat agcctttcct ttatcgcaat     7080

gatggcattt gtaggtgcca ccttcctttt ctactgtcct tttgatgaag tgacagatag     7140

ctgggcaatg gaatccgagg aggtttcccg atattaccct ttgttgaaaa gtctcaatag     7200

ccctttggtc ttctgagact gtatctttga tattcttgga gtagacgaga gtgtcgtgct     7260

ccaccatgtt atcacatcaa tccacttgct ttgaagacgt ggttggaacg tcttcttttt     7320

ccacgatgct cctcgtgggt gggggtccat ctttgggacc actgtcggca gaggcatctt     7380

gaacgatagc ctttccttta tcgcaatgat ggcatttgta ggtgccacct tccttttcta     7440

ctgtcctttt gatgaagtga cagatagctg ggcaatggaa tccgaggagg tttcccgata     7500

ttaccctttg ttgaaaagtc tcaatagccc tttggtcttc tgagactgta tctttgatat     7560

tcttggagta gacgagagtg tcgtgctcca ccattacata ggcccatcgg agctaacgca     7620

gtgaattcag aaatctcaaa attccggcag aacaattttg aatctcgatc cgtagaaacg     7680

agacggtcat tgttttagtt ccaccacgat tatatttgaa atttacgtga gtgtgagtga     7740

gacttgcata agaaaataaa atctttagtt gggaaaaaat tcaataatat aaatgggctt     7800

gagaaggaag cgagggatag gcctttttct aaaataggcc catttaagct attaacaatc     7860

ttcaaaagta ccacagcgct taggtaaaga aagcagctga gtttatatat ggttagagac     7920

gaagtagtga ttgggactat gttcttaatc agttttagag ctagaaatag caagttaaaa     7980

taaggctagt ccgttatcaa cttgaaaaag tggcaccgag tcggtgcttt ttttatcacg     8040

aaagcttact gcgttagctc cgatgggcct atgtaatggt ggagcacgac actctcgtct     8100

actccaagaa tatcaaagat acagtctcag aagaccaaag ggctattgag acttttcaac     8160

aaagggtaat atcgggaaac ctcctcggat tccattgccc agctatctgt cacttcatca     8220

aaaggacagt agaaaaggaa ggtggcacct acaaatgcca tcattgcgat aaaggaaagg     8280

ctatcgttca agatgcctct gccgacagtg gtcccaaaga tggaccccca cccacgagga     8340

gcatcgtgga aaaagaagac gttccaacca cgtcttcaaa gcaagtggat tgatgtgata     8400

acatggtgga gcacgacact ctcgtctact ccaagaatat caaagataca gtctcagaag     8460

accaaagggc tattgagact tttcaacaaa gggtaatatc gggaaacctc ctcggattcc     8520

attgcccagc tatctgtcac ttcatcaaaa ggacagtaga aaaggaaggt ggcacctaca     8580

aatgccatca ttgcgataaa ggaaaggcta tcgttcaaga tgcctctgcc gacagtggtc     8640

ccaaagatgg acccccaccc acgaggagca tcgtggaaaa agaagacgtt ccaaccacgt     8700

cttcaaagca agtggattga tgtgatatct ccactgacgt aagggatgac gcacaatccc     8760

actatccttc gcaagacctt cctctatata aggaagttca tttcatttgg agaggacacg     8820

ctgaaatcac cagtctctct ctacaaatct atctcttaat acgactcact atagggagac     8880

ccaagctggc tagcaacaat ggataagaag tactctatcg gactcgatat cggaactaac     8940

tctgttggat gggctgtgat caccgatgag tacaaggtgc catctaagaa gttcaaggtt     9000

ctcggaaaca ccgataggca ctctatcaag aaaaacctta tcggtgctct cctcttcgat     9060

tctggtgaaa ctgctgaggc taccagactc aagagaaccg ctagaagaag gtacaccaga     9120

agaaagaaca ggatctgcta cctccaagag attttctcta acgagatggc taaagtggat     9180

gattcattct tccacaggct cgaagagtca ttcctcgtgg aagaagataa gaagcacgag     9240

aggcacccta tcttcggaaa catcgttgat gaggtggcat accacgagaa gtaccctact     9300

atctaccacc tcagaaagaa gctcgttgat tctactgata aggctgatct caggctcatc     9360

tacctcgctc tcgctcacat gatcaagttc agaggacact tcctcatcga gggtgatctc     9420

aaccctgata actctgatgt ggataagttg ttcatccagc tcgtgcagac ctacaaccag     9480

cttttcgaag agaaccctat caacgcttca ggtgtggatg ctaaggctat cctctctgct     9540

aggctctcta agtcaagaag gcttgagaac ctcattgctc agctccctgg tgagaagaag     9600

aacggacttt tcggaaactt gatcgctctc tctctcggac tcacccctaa cttcaagtct     9660

aacttcgatc tcgctgagga tgcaaagctc cagctctcaa aggataccta cgatgatgat     9720

ctcgataacc tcctcgctca gatcggagat cagtacgctg atttgttcct cgctgctaag     9780

aacctctctg atgctatcct cctcagtgat atcctcaggg tgaacaccga gatcaccaag     9840

gctccacttt ctgcttctat gatcaagaga tacgatgagc accaccagga tctcacactt     9900

ctcaaggctc ttgttagaca gcagctccca gagaagtaca aagaaatctt cttcgatcag     9960

tctaagaacg gatacgctgg ttacatcgat ggtggtgcat ctcaagaaga gttctacaag    10020

ttcatcaagc caatcttgga gaagatggat ggaaccgagg aactcctcgt gaagctcaat    10080

agagaggatc tccttaggaa gcagaggacc ttcgataacg gatctatccc tcatcagatc    10140

cacctcggag agttgcacgc tatccttaga aggcaagagg atttctaccc attcctcaag    10200

gataacagag agaagattga gaagatcctc accttcagaa tcccttacta cgtgggacct    10260

ctcgctagag gaaactcaag attcgcttgg atgaccagaa agtctgagga aaccatcacc    10320

ccttggaact tcgaagaggt ggtggataag ggtgctagtg ctcagtcttt catcgagagg    10380

atgaccaact tcgataagaa ccttcctaac gagaaggtgc tccctaagca ctctttgctc    10440

tacgagtact tcaccgtgta caacgagttg accaaggtta agtacgtgac cgagggaatg    10500

aggaagcctg cttttttgtc aggtgagcaa aagaaggcta tcgttgatct cttgttcaag    10560

accaacagaa aggtgaccgt gaagcagctc aaagaggatt acttcaagaa aatcgagtgc    10620

ttcgattcag tggaaatctc tggtgttgag gataggttca acgcatctct cggaacctac    10680

cacgatctcc tcaagatcat taaggataag gatttcttgg ataacgagga aaacgaggat    10740

atcttggagg atatcgttct taccctcacc ctcttcgagg atagagagat gatagaagaa    10800

aggctcaaga cctacgctca tctcttcgat gataaggtga tgaagcagtt gaagagaaga    10860

agatacactg gttggggaag gctctcaaga aagctcatta acggaatcag ggataagcag    10920

tctggaaaga caatccttga tttcctcaag tctgatggat tcgctaacag aaacttcatg    10980

cagctcatcc acgatgattc tctcaccttt aaagaggata tccagaaggc tcaggtttca    11040

ggacagggtg atagtctcca tgagcatatc gctaacctcg ctggatcccc tgcaatcaag    11100

aagggaatcc tccagactgt gaagattgtg gatgagttgg tgaaggtgat gggacacaag    11160

cctgagaaca tcgtgatcga aatggctaga gagaaccaga ccactcagaa gggacagaag    11220

aactctaggg aaaggatgaa gaggatcgag gaaggtatca aagagcttgg atctcagatc    11280

ctcaaagagc accctgttga gaacactcag ctccagaacg agaagctcta cctctactac    11340

ttgcagaacg gaagggatat gtatgtggat caagagcttg atattaacag gctctctgat    11400

tacgatgttg atcatatcgt gccacagtct tttatcaaag atgattctat cgataacaag    11460

gtgctcacta ggtctgataa gaacaggggt aagagtgata acgtgccaag tgaagaggtt    11520

gtgaagaaaa tgaagaacta ttggaggcag ctcctcaacg ctaagctcat cactcagaga    11580

aagttcgata acttgaccaa ggctgagagg ggaggactct ctgaattgga taaggcagga    11640

ttcatcaaga gacagctcgt ggaaaccagg cagatcacca aacatgtggc acagatcctc    11700

gattctagga tgaacaccaa gtacgatgag aacgataagt tgatcaggga agtgaaggtt    11760

atcaccctca agtcaaagct cgtgtctgat ttcagaaagg atttccaatt ctacaaggtg    11820

agggaaatca acaactacca ccacgctcac gatgcttacc ttaacgctgt tgttggaacc    11880

gctctcatca agaagtatcc aaagttggag tctgagttcg tgtacggtga ttataaggtg    11940

tacgatgtga ggaagatgat cgctaagtct gagcaagaga tcggaaaggc taccgctaag    12000

tatttcttct actctaacat catgaatttc ttcaagaccg agatcactct cgctaacggt    12060

gagatcagaa agaggccact catcgagaca aacggtgaaa caggtgagat cgtgtgggat    12120

aagggaaggg atttcgctac cgttagaaag gtgctctcta tgcctcaggt gaacatcgtt    12180

aagaaaaccg aggtgcagac cggtggattc tctaaagagt ctatcctccc taagaggaac    12240

tctgataagc tcattgctag gaagaaggat tgggacccta agaaatacgg tggtttcgat    12300

tctcctaccg tggcttactc tgttctcgtt gtggctaagg ttgagaaggg aaagagtaag    12360

aagctcaagt ctgttaagga acttctcgga atcactatca tggaaaggtc atctttcgag    12420

aagaacccaa tcgatttcct tgaggctaag ggatacaaag aggttaagaa ggatctcatc    12480

atcaagctcc caaagtactc acttttcgag ttggagaacg gtagaaagag gatgctcgct    12540

tctgctggtg agcttcaaaa gggaaacgag cttgctctcc catctaagta cgttaacttt    12600

ctttacctcg cttctcacta cgagaagttg aagggatctc cagaagataa cgagcagaag    12660

caacttttcg ttgagcagca caagcactac ttggatgaga tcatcgagca gatcagtgag    12720

ttctctaaaa gggtgatcct cgctgatgca aacctcgata aggtgttgtc tgcttacaac    12780

aagcacagag ataagcctat cagggaacag gcagagaaca tcatccatct cttcaccctt    12840

accaacctcg gtgctcctgc tgctttcaag tacttcgata caaccatcga taggaagaga    12900

tacacctcta ccaaagaagt gctcgatgct accctcatcc atcagtctat cactggactc    12960

tacgagacta ggatcgatct ctcacagctt ggaggtgatc ctaagaagaa aagaaaggtt    13020

agatcttgat gacccgggtc tccataataa tgtgtgagta gttcccagat aagggaatta    13080

gggttcctat agggtttcgc tcatgtgttg agcatataag aaacccttag tatgtatttg    13140

tatttgtaaa atacttctat caataaaatt tctaattcct aaaaccaaaa tccagtacta    13200

aaatccagat cccccgaatt aaggccttga caggatatat tggcgggtaa acctaagaga    13260

aaagagcgtt tattagaata acggatattt aaaactcgag                          13300


<210>  60
<211>  3228
<212>  DNA
<213>  Artificial sequence

<220>
<223>  DNA repair template targeting BAR expression casstte into the 
       gl1-1 crispR targeted site

<400>  60
ccgaaaatta gaggagatta atttggtctc ttatctacta taatgttcga acttgtgact       60

tgaaaattct cattttgact aactaatcag tatacaggct aaactatctg attagattta      120

agaaattaaa tcgatatgat atgtaaatga acacataaat tttgtggtct atttttaaaa      180

ctcaattaaa gagggtaacc tcatgaactt ttaaaaagta tgtcgaaaag ttgactataa      240

tttgagaaac aagacatatg gaaaggagaa ccatttcatt gatccatcaa agtttctttt      300

ttcatgtttt atcttctgca aaaaaactgt tttagggtga actctgtggt acttttaaat      360

aggttgttcc gatcccactt tgttgtataa ataactaaaa ataatatgta gttccaatga      420

aaaagatcat tatcaaatat tagtagaatg taagtgtata cagtgcataa acaaactggt      480

taacgtgtca aataaattac aaatcagtta aatcatacaa tatagaacat ttcgcagtaa      540

tctgccccat atttagtaga tttgtttttg taaatttcat gtcgcaagta tagtatttag      600

atattgccag aggagaaaaa acaatttttt tataaatttt ttggccatag atacaattaa      660

accaactgcc aaattttagc ttttaatacg taacgtaggg taacaaagga taacgctata      720

gtcgctatct atacattatt aggtacatat ttatgcttat agccatgatt acacaaagat      780

acgtattgat gtgagtgtat atataagcac gtgtcacgaa aacccatcat aagttcacta      840

ctcataatct ctttctctct ctcacacaca cacacagaca cacacaaaca tagaaggaat      900

cagagaataa aaaaagaatg agaataagga gaagagatga aaaagagaat caagaataca      960

agaaaggttt atggacagtt gaagaagaca acatccttat ggactatgtt cttaacatgg     1020

tggagcacga cactctcgtc tactccaaga atatcaaaga tacagtctca gaagaccaga     1080

gggctattga gacttttcaa caaagggtaa tatcgggaaa cctcctcgga ttccattgcc     1140

cagctatctg tcacttcatc gaaaggacag tagaaaagga agatggcttc tacaaatgcc     1200

atcattgcga taaaggaaag gctatcgttc aagatgcctc taccgacagt ggtcccaaag     1260

atggaccccc acccacgagg aacatcgtgg aaaaagaaga cgttccaacc acgtcttcaa     1320

agcaagtgga ttgatgtgat atctccactg acgtaaggga tgacgcacaa tcccactatc     1380

cttcgcaaga cccttcctct atataaggaa gttcatttca tttggagagg acctcgagtg     1440

gccaccatgg gcccagaacg acgcccggcc gacatccgcc gtgccaccga ggcggacatg     1500

ccggcggtct gcaccatcgt caaccactac atcgagacaa gcacggtcaa cttccgtacc     1560

gagccgcagg aaccgcagga gtggacggac gacctcgtcc gtctgcggga gcgctatccc     1620

tggctcgtcg ccgaggtgga cggcgaggtc gccggcatcg cctacgcggg cccctggaag     1680

gcacgcaacg cctacgactg gacggccgag tccaccgtgt acgtctcccc ccgccaccag     1740

cggacgggac tgggctccac gctctacacc cacctgctga agtccctgga ggcacagggc     1800

ttcaagagcg tggtcgctgt catcgggctg cccaacgacc cgagcgtgcg catgcacgag     1860

gcgctcggat atgccccccg cggcatgctg cgggcggccg gcttcaagca cgggaactgg     1920

catgacgtgg gtttctggca gctggacttc agcctgccgg taccgccccg tccggtcctg     1980

cccgtcaccg agatctgaga tcacgcgttc tagtccgcaa aaatcaccag tctctctcta     2040

caaatctatc tctctctatt tttctccaga ataatgtgtg agtagttccc agataaggga     2100

attagggttc ttatagggtt tcgctcatgt gttgagcata taagaaaccc ttagtatgta     2160

tttgtatttg taaaatactt ctatcaataa aatttctaat tcctaaaacc aaaatccagt     2220

gatcatggca ctggccaatg gaaccgcatc gtcagaaaaa ctggtactct ttctctctct     2280

cgcgcgtttt gtgattatat atattaactt tagtctctag ctagttcata tataaatata     2340

tctcagttag actaaatttg attcgttgat agggctaaag agatgtggga aaagttgtag     2400

actgagatgg atgaattatt tgagccctaa tgtgaacaaa ggcaatttca ctgaacaaga     2460

agaagacctc attattcgtc tccacaagct cctcggcaat aggtacataa ctttatgtta     2520

ctacaatatt atacaccatg tctttttatt taatcgatcg ttaactgatt ttttctttcg     2580

tcaaatattt gaattgttgt tttaaacaaa gatttggccg gttaagttga tttttaaaaa     2640

tctaattgaa ggattttctt acttgcaaat gtaaaaataa agagagttga caagtaaaga     2700

ctgttttatt tcaattaaac tatgtgttcg tggattattt cacatttcaa aaggtgtttt     2760

cttcacaaaa taaatcttaa aaataatcct ttgaacttaa aatatgttca aaattttcaa     2820

gttgatatat ttttctgaag aagaaaagag ttgcacaaga taaaagtttg tcttctcttc     2880

aatgaaactc aaccgatgtg ttggtgggtt atttcaattt taaaaggtgt ttcctccaaa     2940

aacaaatctt taagagtatc ttttgaactt aaaaatgttc gaacatttca agtggatata     3000

tttaatgaat aatagacact ttttcataag tgtattagtc gtgcaaacag tcacttctca     3060

aaattcatta tagagttaga aacgttttcc acaattgaaa aataaaaata aaaaatcctc     3120

tctatgtata taaatatgca ttcatttatg aatcacatac agatgtcgaa ataataaaaa     3180

gaatcataaa catatggtat gttataattt atgttatatg gtatgaat                  3228


<210>  61
<211>  20
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Single strand DNA oligonucleotide

<400>  61
agacactgac atgggttggt                                                   20


<210>  62
<211>  20
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Single strand DNA oligonucleotide

<400>  62
ccaccaacac atcggttgag                                                   20


<210>  63
<211>  5424
<212>  DNA
<213>  Artificial sequence

<220>
<223>  DNA repair template targeting UBI:MoPAT:PinII expression casstte 
       into the LIGCas-3 crispR targeted site

<400>  63
cccatagaaa actgtgtgct ataatacacc aaaaggaaag caaagtgaaa aggaaacttt       60

gaatagccaa gaagactcgg agtgcttcac gccttcacct atcccacata ggtgatgagc      120

taagagtaaa atgtagattc tctcgagtac tgaatattgc ctgcactttt ccttgcagta      180

aatacacctt taatccatga cgagagtcca ctctttgagt ccgtcttgag attcttccat      240

tgatcataca acatgacctc gaagtcctga tggagaacaa cttatataat taaaactaca      300

atacagaaag ttcctgacaa ttaaaacctt tggtggtggc atgccgtagg ttaaaaaaaa      360

tagataatga caacacaact ggagacacgc tctttgccga gtgctcacac gtttgctgag      420

agcgagcact cggcaaatat atgatttgcc gaataccacc ctcctcggca aaacaataca      480

ctaggcaaaa aggtagtttc ccatcaccat gatgcccgcc gttaatgtac cttctatgcc      540

gagtatgttg gcgctcagca aagagatcgt taccggcgtt tgtttcacca agagctcttt      600

gacgagtgtg gcacacgaca aaaccttttg ccgagtgtaa ttagtcgttt gccaagtgac      660

tggtgcagtt ggcaaaggag tcgtttatta tgtgtgggca aaatgatata tggtgccagt      720

tagggctagc aaattaaagg gggggggggg ggggttaggt tgaagaaggt gacgagtaat      780

aaggtctcgg acggccgcgc gcatatatat cagatccgat ccaatggcac acggtgcaaa      840

cgaaaagcac gaaatttcca ccagcttaat tagggagaga aaaatagagc accagctgat      900

gagtgaatga atgagataga cgggacacag agggtccagc aggctagcct actctggccg      960

ccctaaatag aagtcagtgc cgtgacgacg cgcaaacttc ttttgatcgg ctgcggaaat     1020

aatatactgt aacgatttac gcacctgctg ggaattgtac cgtacgtgcc ccggcggagg     1080

atatatatac ctcacacaag ggcgaattgt actagttagt tagctagtcg gtcctagatg     1140

ccgtaatcat tagctaatcg taagtgacgc ttggacacga gcggcttgag ctaggaacct     1200

acgaagtcat cggaatcagc tcaggtgtac agaagttcct atactttctg gagaatagga     1260

acttcggaat aggaacttcg tatacgctag ggccgcattc gcaaaacaca cctagactag     1320

atttgttttg ctaacccaat tgatattaat tatatatgat taatatttat atgtatatgg     1380

atttggttaa tgaaatgcat ctggttcatc aaagaattat aaagacacgt gacattcatt     1440

taggataaga aatatggatg atctctttct cttttattca gataactagt aattacacat     1500

aacacacaac tttgatgccc acattatagt gattagcatg tcactatgtg tgcatccttt     1560

tatttcatac attaattaag ttggccaatc cagaagatgg acaagtctag gtttcgactc     1620

agatctgcgt caccgggcgc accgggcgcg gcggggccgg cagctcgaag tcgcgctgcc     1680

agaagccgac gtcgtgccag ccgccgtgct tgtagccggc ggcgcggagg gtgccgcggg     1740

cggtgtagcc gagggcctcg tggaggcgca cggacgggtc gttcgggagg ccgatcacgg     1800

ccaccacgga cttgaagccc tgggcctcca tgctcttgag gaggtgggtg tagagggtgg     1860

agccgaggcc gaggcgctgg tggcggtggg acacgtacac ggtggactcc acggtccagt     1920

cgtaggcgtt gcgggccttc cacgggccgg cgtaggcgat gccggccacc acgccctcca     1980

cctcggccac gagccacggg tagcggtcct ggaggcgctc caggtcgtcg atccactcct     2040

gcggggtctg cggctcggtg cggaagttca cggtggaggt ctcgatgtag tggttcacga     2100

tgtcgcacac ggcggccatg tcggcggcgg tggccgggcg gatctcgacg gggcggcgct     2160

cgggggacat ggtgtcgtgt ggatcccggt ggatctgaag ttcctatact ttctagagaa     2220

taggaacttc ggaataggaa cttcgctagc gaattgatcc tctagagtcg acctgcagaa     2280

gtaacaccaa acaacagggt gagcatcgac aaaagaaaca gtaccaagca aataaatagc     2340

gtatgaaggc agggctaaaa aaatccacat atagctgctg catatgccat catccaagta     2400

tatcaagatc aaaataatta taaaacatac ttgtttatta taatagatag gtactcaagg     2460

ttagagcata tgaatagatg ctgcatatgc catcatgtat atgcatcagt aaaacccaca     2520

tcaacatgta tacctatcct agatcgatat ttccatccat cttaaactcg taactatgaa     2580

gatgtatgac acacacatac agttccaaaa ttaataaata caccaggtag tttgaaacag     2640

tattctactc cgatctagaa cgaatgaacg accgcccaac cacaccacat catcacaacc     2700

aagcgaacaa aaagcatctc tgtatatgca tcagtaaaac ccgcatcaac atgtatacct     2760

atcctagatc gatatttcca tccatcatct tcaattcgta actatgaata tgtatggcac     2820

acacatacag atccaaaatt aataaatcca ccaggtagtt tgaaacagaa ttctactccg     2880

atctagaacg accgcccaac cagaccacat catcacaacc aagacaaaaa aaagcatgaa     2940

aagatgaccc gacaaacaag tgcacggcat atattgaaat aaaggaaaag ggcaaaccaa     3000

accctatgca acgaaacaaa aaaaatcatg aaatcgatcc cgtctgcgga acggctagag     3060

ccatcccagg attccccaaa gagaaacact ggcaagttag caatcagaac gtgtctgacg     3120

tacaggtcgc atccgtgtac gaacgctagc agcacggatc taacacaaac acggatctaa     3180

cacaaacatg aacagaagta gaactaccgg gccctaacca tgcatggacc ggaacgccga     3240

tctagagaag gtagagaggg ggggggggga ggacgagcgg cgtaccttga agcggaggtg     3300

ccgacgggtg gatttggggg agatctggtt gtgtgtgtgt gcgctccgaa caacacgagg     3360

ttggggaaag agggtgtgga gggggtgtct atttattacg gcgggcgagg aagggaaagc     3420

gaaggagcgg tgggaaagga atcccccgta gctgccggtg ccgtgagagg aggaggaggc     3480

cgcctgccgt gccggctcac gtctgccgct ccgccacgca atttctggat gccgacagcg     3540

gagcaagtcc aacggtggag cggaactctc gagaggggtc cagaggcagc gacagagatg     3600

ccgtgccgtc tgcttcgctt ggcccgacgc gacgctgctg gttcgctggt tggtgtccgt     3660

tagactcgtc gacggcgttt aacaggctgg cattatctac tcgaaacaag aaaaatgttt     3720

ccttagtttt tttaatttct taaagggtat ttgtttaatt tttagtcact ttattttatt     3780

ctattttata tctaaattat taaataaaaa aactaaaata gagttttagt tttcttaatt     3840

tagaggctaa aatagaataa aatagatgta ctaaaaaaat tagtctataa aaaccattaa     3900

ccctaaaccc taaatggatg tactaataaa atggatgaag tattatatag gtgaagctat     3960

ttgcaaaaaa aaaggagaac acatgcacac taaaaagata aaactgtaga gtcctgttgt     4020

caaaatactc aattgtcctt tagaccatgt ctaactgttc atttatatga ttctctaaaa     4080

cactgatatt attgtagtac tatagattat attattcgta gagtaaagtt taaatatatg     4140

tataaagata gataaactgc acttcaaaca agtgtgacaa aaaaaatatg tggtaatttt     4200

ttataactta gacatgcaat gctcattatc tctagagagg ggcacgaccg ggtcacgctg     4260

cactgcaggc tagcggcgaa ttcgcccttg tacgcgtacg cgtatatata cgtgcgctgc     4320

tactcatttg cgcgggaata cagctcagtc tgctgtgcgc tgcaggatgt acatacatac     4380

atgcgcaggt gcaaagtcta cgcgcgcggg caatgcaagc ccctggcgta gttgggccat     4440

gactgagatc acgcctcatg gtcatggaac gaaacaccgc gtccggccgg gctgcccctg     4500

gcgtcacgcg ggaggcagct gctagcgtta gcgtacgtac ccaccgtctc gtacacacca     4560

ccgcagggag agagaagagc gatgcaatgc acatgtacag catccgcatc atgcatagat     4620

actcatatct tcaaggccac acatgcagca gtgtcgtacg ctacgttgtt tcaacggagg     4680

aggaggatac atacatagac acccacagcc agcctagcat atagcagata gcatacggac     4740

tcccgggtga ggaaaaatgg agggcgaacc aaaccaacca caaagaagca gcagcagcag     4800

cagcagcagc tgcggctgct atcaccactc accaactcca attaaagatc tctctctctc     4860

tctctactgg ccggccctgt cagtgccagc gcccggtttg ttgctagctg agctgcgggc     4920

gtcgctctta gatatagccc aaaactcact ccaccaccac tcgttccatg gaaccctaga     4980

ccaaaagtac tcgcgctctc ggccctcgct ctcgccctct ccctctccgc agcaaaagag     5040

atccggccgg ccgagaaggg cgcgcgctag ctgcccggct actagctggc gcccgcccgc     5100

gcatatatct gtgtcatcgc catcacccac accatggccc ggccggccaa caccgccgta     5160

ttagctctgt ctgtcgctcg tccacctgcg accgactgag cgatcgatct ccaccgagct     5220

ctccgctaag cgctgtcctt gccgccgtcc tcccctccgt cccctacgca tccatttccg     5280

tgtgctcgtg tgtgcgcgcg cgggcactcc tgctcctgct ccctccggcc cctcctcccc     5340

tcccaggctc ccagctagcc gcgcccgccc gcgcgacctg cacctgcaca gatcgggcgg     5400

ccgggccgac cgatcgatcg agat                                            5424


<210>  64
<211>  20
<212>  DNA
<213>  Zea mays


<220>
<221>  misc_feature
<223>  Lig-CR3 gRNA target sequence

<400>  64
gcgtacgcgt acgtgtgagg                                                   20


<210>  65
<211>  23
<212>  DNA
<213>  Zea mays

<400>  65
gcggcgagaa atttgaccag cgg                                               23


<210>  66
<211>  22
<212>  DNA
<213>  Zea mays

<400>  66
gcgcaccgtc ggtttcatag gg                                                22


<210>  67
<211>  15489
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Binary plasmid expressing two gRNAs targeting Mate1 flanking 
       regions to induce structural variations

<400>  67
ggcaggatat attcaattgt aaatggcttc atgtccggga aatctacatg gatcagcaat       60

gagtatgatg gtcaatatgg agaaaaagaa agagtaatta ccaatttttt ttcaattcaa      120

aaatgtagat gtccgcagcg ttattataaa atgaaagtac attttgataa aacgacaaat      180

tacgatccgt cgtatttata ggcgaaagca ataaacaaat tattctaatt cggaaatctt      240

tatttcgacg tgtctacatt cacgtccaaa tgggggctta gatgagaaac ttcacgatcg      300

gctctagatc gggccaacat ggtggagcac gacactctcg tctactccaa gaatatcaaa      360

gatacagtct cagaagacca aagggctatt gagacttttc aacaaagggt aatatcggga      420

aacctcctcg gattccattg cccagctatc tgtcacttca tcaaaaggac agtagaaaag      480

gaaggtggca cctacaaatg ccatcattgc gataaaggaa aggctatcgt tcaagatgcc      540

tctgccgaca gtggtcccaa agatggaccc ccacccacga ggagcatcgt ggaaaaagaa      600

gacgttccaa ccacgtcttc aaagcaagtg gattgatgtg aacatggtgg agcacgacac      660

tctcgtctac tccaagaata tcaaagatac agtctcagaa gaccaaaggg ctattgagac      720

ttttcaacaa agggtaatat cgggaaacct cctcggattc cattgcccag ctatctgtca      780

cttcatcaaa aggacagtag aaaaggaagg tggcacctac aaatgccatc attgcgataa      840

aggaaaggct atcgttcaag atgcctctgc cgacagtggt cccaaagatg gacccccacc      900

cacgaggagc atcgtggaaa aagaagacct tccaaccacg tcttcaaagc aagtggattg      960

atgtgatatc tccactgacg taagggatga cgcacaatcc cactatcctt cgcaagaccc     1020

ttcctctata taaggaagtt catttcattt ggagaggaca cgctgaaatc accagtctct     1080

ctctacaaat ctatctctct cgagtggcca ccatgggccc agaacgacgc ccggccgaca     1140

tccgccgtgc caccgaggcg gacatgccgg cggtctgcac catcgtcaac cactacatcg     1200

agacaagcac ggtcaacttc cgtaccgagc cgcaggaacc gcaggagtgg acggacgacc     1260

tcgtccgtct gcgggagcgc tatccctggc tcgtcgccga ggtggacggc gaggtcgccg     1320

gcatcgccta cgcgggcccc tggaaggtaa gtttctgctt ctacctttga tatatatata     1380

ataattatca ttaattagta gtaatataat atttcaaata tttttttcaa aataaaagaa     1440

tgtagtatat agcaattgct tttctgtagt ttataagtgt gtatatttta atttataact     1500

tttctaatat atgaccaaaa tttgttgatg tgcaggcacg caacgcctac gactggacgg     1560

ccgagtccac cgtgtacgtg tccccccgcc accagcggac gggactgggc tccacgctct     1620

acacccacct gctgaagtcc ctggaggcac agggcttcaa gagcgtggtc gctgtcatcg     1680

ggctgcccaa cgacccgagc gtgcgcatgc acgaggcgct cggatatgcc ccccgcggca     1740

tgctgcgggc ggccggcttc aagcacggga actggcatga cgtgggtttc tggcagctgg     1800

acttcagcct gccggtaccg ccccgtccgg tcctgcccgt caccgagatc tgagatcacg     1860

cgttcgagta ttatggcatt gggaaaactg tttttcttgt accatttgtt gtgcttgtaa     1920

tttactgtgt tttttattcg gttttcgcta tcgaactgtg aaatggaaat ggatggagaa     1980

gagttaatga atgatatggt ccttttgttc attctcaaat taatattatt tgttttttct     2040

cttatttgtt gtgtgttgaa tttgaaatta taagagatat gcaaacattt tgttttgagt     2100

aaaaatgtgt caaatcgtgg cctctaatga ccgaagttaa tatgaggagt aaaacactga     2160

agcctgcagg catgcaagct gatccactag aggccatggc ggccgcacta gtctgcagtg     2220

cagcgtgacc cggtcgtgcc cctctctaga gataatgagc attgcatgtc taagttataa     2280

aaaattacca catatttttt ttgtcacact tgtttgaagt gcagtttatc tatctttata     2340

catatattta aactttactc tacgaataat ataatctata gtactacaat aatatcagtg     2400

ttttagagaa tcatataaat gaacagttag acatggtcta aaggacaatt gagtattttg     2460

acaacaggac tctacagttt tatcttttta gtgtgcatgt gttctccttt ttttttgcaa     2520

atagcttcac ctatataata cttcatccat tttattagta catccattta gggtttaggg     2580

ttaatggttt ttatagacta atttttttag tacatctatt ttattctatt ttagcctcta     2640

aattaagaaa actaaaactc tattttagtt tttttattta ataatttaga tataaaatag     2700

aataaaataa agtgactaaa aattaaacaa atacccttta agaaattaaa aaaactaagg     2760

aaacattttt cttgtttcga gtagataatg ccagcctgtt aaacgccgtc gatcgacgag     2820

tctaacggac accaaccagc gaaccagcag cgtcgcgtcg ggccaagcga agcagacggc     2880

acggcatctc tgtcgctgcc tctggacccc tctcgagagt tccgctccac cgttggactt     2940

gctccgctgt cggcatccag aaattgcgtg gcggagcggc agacgtgagc cggcacggca     3000

ggcggcctcc tcctcctctc acggcaccgg cagctacggg ggattccttt cccaccgctc     3060

cttcgctttc ccttcctcgc ccgccgtaat aaatagacac cccctccaca ccctctttcc     3120

ccaacctcgt gttgttcgga gcgcacacac acacaaccag atctccccca aatccacccg     3180

tcggcacctc cgcttcaagg tacgccgctc gtcctccccc cccccccctc tctaccttct     3240

ctagatcggc gttccggtcc atggttaggg cccggtagtt ctacttctgt tcatgtttgt     3300

gttagatccg tgtttgtgtt agatccgtgc tgctagcgtt cgtacacgga tgcgacctgt     3360

acgtcagaca cgttctgatt gctaacttgc cagtgtttct ctttggggaa tcctgggatg     3420

gctctagccg ttccgcagac gggatcgatc taggataggt atacatgttg atgtgggttt     3480

tactgatgca tatacatgat ggcatatgca gcatctattc atatgctcta accttgagta     3540

cctatctatt ataataaaca agtatgtttt ataattattt tgatcttgat atacttggat     3600

gatggcatat gcagcagcta tatgtggatt tttttagccc tgccttcata cgctatttat     3660

ttgcttggta ctgtttcttt tgtcgatgct caccctgttg tttggtgtta cttctgcagg     3720

tactagatcc tccaccgaaa tggcctccgg acccaagaag aagcgcaaag tgggaatcca     3780

cggagctcca gacaagaagt acagcattgg tctggacatc gggaccaact ccgtcggctg     3840

ggccgttatc accgatgagt acaaagtgcc ttctaagaag ttcaaagtcc tcggaaacac     3900

tgacaggcac agcatcaaga agaacttgat tggcgccctt ctcttcgact ccggcgaaac     3960

cgctgaggcc acccgtctga agcgcaccgc cagaaggcgc tacacccgtc gcaagaatag     4020

gatctgctac ctccaggaga tcttcagcaa cgagatggcc aaagtggacg attccttctt     4080

tcaccgtttg gaggagagct tccttgtcga ggaggacaag aagcacgagc gccatcccat     4140

cttcggaaac attgttgacg aggtggctta ccacgagaag tatccaacta tctaccacct     4200

cagaaagaag ctggtggact ccaccgacaa ggccgatttg aggctcatct accttgccct     4260

cgctcacatg atcaagttcc gcggtcactt cctgattgag ggcgacctca accccgacaa     4320

ctctgacgtc gataagttgt tcatccaact ggtccagacc tacaaccagc ttttcgagga     4380

gaatcctatc aacgcctccg gggttgacgc caaggctatt ctcagcgccc gtctgtccaa     4440

gagccgcagg cttgagaact tgatcgccca gctcccagga gagaagaaga acggcctctt     4500

cggaaacctg atcgctctct ccttgggcct tacccctaac ttcaagtcta attttgacct     4560

cgccgaggac gccaagctgc aactcagcaa ggacacctac gatgacgact tggacaacct     4620

gctcgcccag atcggcgatc agtacgctga ccttttcttg gccgccaaga acctctccga     4680

cgctattctg ctctccgaca tccttcgcgt gaacactgag atcaccaagg ccccactgag     4740

cgcctccatg atcaagcgtt acgacgaaca ccaccaagat ttgaccctcc tcaaggctct     4800

tgtcagacag cagctgcccg agaagtacaa ggagattttc ttcgaccaga gcaagaacgg     4860

atacgccggt tacatcgacg gcggggcctc ccaagaggag ttctacaagt tcatcaagcc     4920

catcttggag aagatggacg gcaccgaaga gctgctcgtg aagctcaacc gcgaggatct     4980

tttgaggaag cagcgtacct tcgacaacgg atctattccc caccagatcc atctcggcga     5040

gctgcacgct atcctccgca ggcaagagga cttctatcca ttccttaagg acaatcgcga     5100

gaagattgaa aagatcctca cttttagaat cccttactac gtcggaccct tggcccgtgg     5160

caacagccgc ttcgcctgga tgaccaggaa gtccgaggaa accatcaccc catggaactt     5220

cgaggaggtg gttgacaagg gggccagcgc tcagtccttc attgagcgca tgaccaactt     5280

cgataagaac ctgcccaacg agaaagtcct cccaaagcac tcccttctgt acgagtactt     5340

cactgtctac aatgagttga ccaaagtgaa gtacgtgacc gagggtatgc gtaagcctgc     5400

cttcctcagc ggcgagcaga agaaggccat cgttgacctc ctgttcaaga ccaacaggaa     5460

agtcaccgtg aagcagctta aggaggacta cttcaagaag atcgagtgct ttgactctgt     5520

cgagatcagc ggagtggagg atcgcttcaa cgcttccttg ggcacttacc acgacctcct     5580

gaagattatc aaggacaagg acttcctcga caacgaggag aacgaagata tcttggagga     5640

catcgttctt accctcaccc tgttcgagga cagagagatg attgaggaac gcctcaagac     5700

ctacgcccac ttgttcgacg ataaagtcat gaagcaactt aagcgtaggc gctacaccgg     5760

atggggcagg ctgtcccgta agctcatcaa cggtatccgc gacaagcaga gcggcaagac     5820

tattctcgac ttcttgaagt ccgacggatt cgccaatcgc aacttcatgc agctgatcca     5880

cgacgattct cttaccttca aggaggacat ccaaaaggct caagtgtccg gccagggcga     5940

cagcctccac gagcacatcg ccaacctggc cgggagcccc gctattaaga agggaatcct     6000

ccagaccgtc aaggtggttg acgagttggt caaggtgatg ggcagacata agccagagaa     6060

catcgtcatc gagatggcca gggaaaacca gaccacccag aagggacaga agaactcccg     6120

tgagcgcatg aagaggattg aggagggcat caaggagctt ggttcccaga tcctcaagga     6180

gcaccccgtg gaaaacaccc aactccagaa tgagaagctg tatttgtact accttcagaa     6240

cgggcgcgat atgtacgttg accaagagct ggacatcaac cgcctcagcg actacgacgt     6300

ggatcacatt gtccctcagt cctttctcaa ggacgactct atcgacaaca aggtgttgac     6360

tagaagcgat aagaaccgcg gcaagtccga caacgtccca tccgaggagg tggtcaagaa     6420

gatgaagaat tactggaggc agctgcttaa cgccaagctc atcacccagc gcaagttcga     6480

caacttgacc aaggccgagc gtggaggcct cagcgaactg gacaaggctg gattcatcaa     6540

gaggcaactt gttgaaaccc gccagattac caagcacgtg gcccagatcc tcgactcccg     6600

tatgaacact aagtacgatg agaacgacaa gctgatccgc gaggtcaaag tgattaccct     6660

caagagcaag ctcgtgtctg acttcagaaa ggacttccaa ttctacaagg ttagggagat     6720

caacaactac caccacgccc acgatgctta ccttaatgcc gtggtcggca ccgccttgat     6780

caagaagtac cccaagctgg agtccgagtt cgtgtatggt gactacaagg tctacgacgt     6840

tcgcaagatg atcgctaaga gcgagcagga gattggcaag gccaccgcca agtacttctt     6900

ctactccaac atcatgaact tctttaagac cgagatcact ttggctaacg gggagatccg     6960

taagcgccct ctcattgaaa ccaacggaga aaccggcgag atcgtgtggg acaagggcag     7020

ggacttcgcc accgtcagaa aagtgctgag catgccccaa gtcaacatcg tgaagaaaac     7080

cgaggttcag actggaggct tctccaagga gtctatcctc ccaaagcgca attccgataa     7140

gttgattgcc cgtaagaagg actgggaccc caagaagtac ggtggattcg acagcccaac     7200

cgtcgcctac tccgtgcttg tcgtggctaa agttgagaag ggcaagagca agaagctcaa     7260

gtccgtcaag gagctgctcg ggatcaccat catggagcgc agctccttcg agaagaaccc     7320

tattgatttc cttgaggcca agggctacaa ggaagtgaag aaggacttga tcatcaagct     7380

ccccaagtac tctctgttcg agcttgagaa cggaaggaag cgtatgctcg cctccgctgg     7440

cgagctgcaa aagggaaacg agttggccct cccaagcaag tacgtcaact tcctgtacct     7500

cgcctcccac tatgagaagc tcaagggcag ccccgaggac aacgaacaga agcagttgtt     7560

tgtggagcag cataagcact accttgacga gatcattgag cagatcagcg agttctccaa     7620

gcgcgttatc ctggctgacg ccaatctcga taaagtcctt tctgcctaca acaagcacag     7680

ggacaagcct atcagagagc aggctgaaaa cattatccac ttgttcaccc tcaccaacct     7740

gggtgcccca gccgccttca agtacttcga cactaccatc gaccgcaagc gttacacctc     7800

caccaaggaa gtgctcgatg ctacccttat ccaccagagc attactgggt tgtacgaaac     7860

caggatcgac ctgtcccaac tcggcggaga caagcgcccc gccgccacca agaaggctgg     7920

ccaggccaag aagaagaagt aacccgggat cgttcaaaca tttggcaata aagtttctta     7980

agattgaatc ctgttgccgg tcttgcgatg attatcatat aatttctgtt gaattacgtt     8040

aagcatgtaa taattaacat gtaatgcatg acgttattta tgagatgggt ttttatgatt     8100

agagtcccgc aattatacat ttaatacgcg atagaaaaca aaatatagcg cgcaaactag     8160

gataaattat cgcgcgcggt gtcatctatg ttactagatc gctcgaattc gtacagcatt     8220

acgtaggtac gttttctttt tcttcccgga gagatgatac gataatcatg taaacccaga     8280

atttaaaaaa tattctttac tataaaaatt ttaattaggg aacgtattat tttttacatg     8340

acaccttttg agaaagaggg acttgtaata tgggacaaat gaacaatttc taagaaatgg     8400

gcatatgact ctcagtacaa tggaccaaat tccctccagt cggcccagca atacaaaggg     8460

aaagaaatga gggggcccac aggccacggc ccacttttct ccgtggtggg gagatccagc     8520

tagaggtccg gcccacaagt ggcccttgcc ccgtgggacg gtgggattgc agagcgcgtg     8580

ggcggaaaca acagtttagt accacctcgc tcacgcaacg acgcgaccac ttgcttataa     8640

gctgctgcgc tgaggctcag cttgcggcga gaaatttgac caggttttag agctagaaat     8700

agcaagttaa aataaggcta gtccgttatc aacttgaaaa agtggcaccg agtcggtgct     8760

tttttttaga cccagctttc tcgtacgaaa gttggcatta cgctgtacag cattacgtag     8820

gtacgttttc tttttcttcc cggagagatg atacgataat catgtaaacc cagaatttaa     8880

aaaatattct ttactataaa aattttaatt agggaacgta ttatttttta catgacacct     8940

tttgagaaag agggacttgt aatatgggac aaatgaacaa tttctaagaa atgggcatat     9000

gactctcagt acaatggacc aaattccctc cagtcggccc agcaatacaa agggaaagaa     9060

atgagggggc ccacaggcca cggcccactt ttctccgtgg tggggagatc cagctagagg     9120

tccggcccac aagtggccct tgccccgtgg gacggtggga ttgcagagcg cgtgggcgga     9180

aacaacagtt tagtaccacc tcgctcacgc aacgacgcga ccacttgctt ataagctgct     9240

gcgctgaggc tcagcttgcg caccgtcggt ttcatagttt tagagctaga aatagcaagt     9300

taaaataagg ctagtccgtt atcaacttga aaaagtggca ccgagtcggt gctttttttg     9360

tcgacaggcc ttaagggcca gatcttgggc ccggtacccg atcagattgt cgtttcccgc     9420

cttcggttta aactatcagt gtttgacagg atatattggc gggtaaacct aagagaaaag     9480

agcgtttatt agaataatcg gatatttaaa agggcgtgaa aaggtttatc cgttcgtcca     9540

tttgtatgtg catgccaacc acagggttcc cctcgggagt gcttggcatt ccgtgcgata     9600

atgacttctg ttcaaccacc caaacgtcgg aaagcctgac gacggagcag cattccaaaa     9660

agatcccttg gctcgtctgg gtcggctaga aggtcgagtg ggctgctgtg gcttgatccc     9720

tcaacgcggt cgcggacgta gcgcagcgcc gaaaaatcct cgatcgcaaa tccgacgctg     9780

tcgaaaagcg tgatctgctt gtcgctcttt cggccgacgt cctggccagt catcacgcgc     9840

caaagttccg tcacaggatg atctggcgcg agttgctgga tctcgccttc aatccgggtc     9900

tgtggcggga actccacgaa aatatccgaa cgcagcaaga tatcgcggtg catctcggtc     9960

ttgcctgggc agtcgccgcc gacgccgttg atgtggacgc cgaaaaggat ctaggtgaag    10020

atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg    10080

tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc    10140

tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag    10200

ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtt    10260

cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac    10320

ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc    10380

gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt    10440

tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt    10500

gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc    10560

ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt    10620

tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca    10680

ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt    10740

tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccga    10800

ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt    10860

cagtgagcga ggaagcggaa gagcgcctga tgcggtattt tctccttacg catctgtgcg    10920

gtatttcaca ccgcatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa    10980

gccagtaacc gtgcggctgc atgaaatcct ggccggtttg tctgatgcca agctcgcggc    11040

ctggccggcg agcttggccg ctgaagaaac cgagcgccgc cgtctaaaaa ggtgatgtgt    11100

atttgagtaa aacagcttgc gtcatgcggt cgctgcgtat atgatgcgat gagtaaataa    11160

acaaatacgc aaggggaacg catgaaggtt atcgctgtac ttaaccagaa aggcgggtca    11220

ggcaagacga ccatcgcaac ccatctagcc cgcgccctgc aactcgccgg ggccgatgtt    11280

ctgttagtcg attccgatcc ccagggcagt gcccgcgatt gggcggccgt gcgggaagat    11340

caaccgctaa ccgttgtcgg catcgaccgc ccgacgattg accgcgacgt gaaggccatc    11400

ggccggcgcg acttcgtagt gatcgacgga gcgccccagg cggcggactt ggctgtgtcc    11460

gcgatcaagg cagccgactt cgtgctgatt ccggtgcagc caagccctta cgacatatgg    11520

gccaccgccg acctggtgga gctggttaag cagcgcattg aggtcacgga tggaaggcta    11580

caagcggcct ttgtcgtgtc gcgggcgatc aaaggcacgc gcatcggcgg tgaggttgcc    11640

gaggcgctgg ccgggtacga gctgcccatt cttgagtccc gtatcacgca gcgcgtgagc    11700

tacccaggca ctgccgccgc cggcacaacc gttcttgaat cagaacccga gggcgacgct    11760

gcccgcgagg tccaggcgct ggccgctgaa attaaatcaa aactcatttg agttaatgag    11820

gtaaagagaa aatgagcaaa agcacaaaca cgctaagtgc cggccgtccg agcgcacgca    11880

gcagcaaggc tgcaacgttg gccagcctgg cagacacgcc agccatgaag cgggtcaact    11940

ttcagttgcc ggcggaggat cacaccaagc tgaagatgta cgcggtacgc caaggcaaga    12000

ccattaccga gctgctatct gaatacatcg cgcagctacc agagtaaatg agcaaatgaa    12060

taaatgagta gatgaatttt agcggctaaa ggaggcggca tggaaaatca agaacaacca    12120

ggcaccgacg ccgtggaatg ccccatgtgt ggaggaacgg gcggttggcc aggcgtaagc    12180

ggctgggttg tctgccggcc ctgcaatggc actggaaccc ccaagcccga ggaatcggcg    12240

tgagcggtcg caaaccatcc ggcccggtac aaatcggcgc ggcgctgggt gatgacctgg    12300

tggagaagtt gaaggcggcg caggccgccc agcggcaacg catcgaggca gaagcacgcc    12360

ccggtgaatc gtggcaagcg gccgctgatc gaatccgcaa agaatcccgg caaccgccgg    12420

cagccggtgc gccgtcgatt aggaagccgc ccaagggcga cgagcaacca gattttttcg    12480

ttccgatgct ctatgacgtg ggcacccgcg atagtcgcag catcatggac gtggccgttt    12540

tccgtctgtc gaagcgtgac cgacgagctg gcgaggtgat ccgctacgag cttccagacg    12600

ggcacgtaga ggtttccgca gggccggccg gcatggcgag tgtgtgggat tacgacctgg    12660

tactgatggc ggtttcccat ctaaccgaat ccatgaaccg ataccgggaa gggaagggag    12720

acaagcccgg ccgcgtgttc cgtccacacg ttgcggacgt actcaagttc tgccggcgag    12780

ccgatggcgg aaagcagaaa gacgacctgg tagaaacctg cattcggtta aacaccacgc    12840

acgttgccat gcagcgtacg aagaaggcca agaacggccg cctggtgacg gtatccgagg    12900

gtgaagcctt gattagccgc tacaagatcg taaagagcga aaccgggcgg ccggagtaca    12960

tcgagatcga gctagctgat tggatgtacc gcgagatcac agaaggcaag aacccggacg    13020

tgctgacggt tcaccccgat tactttttga tcgatcccgg catcggccgt tttctctacc    13080

gcctggcacg ccgcgccgca ggcaaggcag aagccagatg gttgttcaag acgatctacg    13140

aacgcagtgg cagcgccgga gagttcaaga agttctgttt caccgtgcgc aagctgatcg    13200

ggtcaaatga cctgccggag tacgatttga aggaggaggc ggggcaggct ggcccgatcc    13260

tagtcatgcg ctaccgcaac ctgatcgagg gcgaagcatc cgccggttcc taatgtacgg    13320

agcagatgct agggcaaatt gccctagcag gggaaaaagg tcgaaaaggt ctctttcctg    13380

tggatagcac gtacattggg aacccaaagc cgtacattgg gaaccggaac ccgtacattg    13440

ggaacccaaa gccgtacatt gggaaccggt cacacatgta agtgactgat ataaaagaga    13500

aaaaaggcga tttttccgcc taaaactctt taaaacttat taaaactctt aaaacccgcc    13560

tggcctgtgc ataactgtct ggccagcgca cagccgaaga gctgcaaaaa gcgcctaccc    13620

ttcggtcgct gcgctcccta cgccccgccg cttcgcgtcg gcctatcgcg gccgctggcc    13680

gctcaaaaat ggctggccta cggccaggca atctaccagg gcgcggacaa gccgcgccgt    13740

cgccactcga ccgccggcgc ccacatcaag gcaccggtgg gtatgcctga cgatgcgtgg    13800

agaccgaaac cttgcgctcg ttcgccagcc aggacagaaa tgcctcgact tcgctgctgc    13860

ccaaggttgc cgggtgacgc acaccgtgga aacggatgaa ggcacgaacc cagtggacat    13920

aagcctgttc ggttcgtaag ctgtaatgca agtagcgtat gcgctcacgc aactggtcca    13980

gaaccttgac cgaacgcagc ggtggtaacg gcgcagtggc ggttttcatg gcttgttatg    14040

actgtttttt tggggtacag tctatgcctc gggcatccaa gcagcaagcg cgttacgccg    14100

tgggtcgatg tttgatgtta tggagcagca acgatgttac gcagcagggc agtcgcccta    14160

aaacaaagtt aaacatcatg agggaagcgg tgatcgccga agtatcgact caactatcag    14220

aggtagttgg cgtcatcgag cgccatctcg aaccgacgtt gctggccgta catttgtacg    14280

gctccgcagt ggatggcggc ctgaagccac acagtgatat tgatttgctg gttacggtga    14340

ccgtaaggct tgatgaaaca acgcggcgag ctttgatcaa cgaccttttg gaaacttcgg    14400

cttcccctgg agagagcgag attctccgcg ctgtagaagt caccattgtt gtgcacgacg    14460

acatcattcc gtggcgttat ccagctaagc gcgaactgca atttggagaa tggcagcgca    14520

atgacattct tgcaggtatc ttcgagccag ccacgatcga cattgatctg gctatcttgc    14580

tgacaaaagc aagagaacat agcgttgcct tggtaggtcc agcggcggag gaactctttg    14640

atccggttcc tgaacaggat ctatttgagg cgctaaatga aaccttaacg ctatggaact    14700

cgccgcccga ctgggctggc gatgagcgaa atgtagtgct tacgttgtcc cgcatttggt    14760

acagcgcagt aaccggcaaa atcgcgccga aggatgtcgc tgccgactgg gcaatggagc    14820

gcctgccggc ccagtatcag cccgtcatac ttgaagctag acaggcttat cttggacaag    14880

aagaagatcg cttggcctcg cgcgcagatc agttggaaga atttgtccac tacgtgaaag    14940

gcgagatcac caaggtagtc ggcaaataat gtctaacaat tcgttcaagc cgacgccgct    15000

tcgcggcgcg gcttaactca agcgttagat gcactaagca cataattgct cacagccaaa    15060

ctatcaggtc aagtctgctt ttattatttt taagcgtgca taataagccc tacacaaatt    15120

gggagatata tcatgaaagg ctggcttttt cttgttatcg caatagttgg cgaagtaatc    15180

gcaacatagc ttgcttggtc gttccgcgtg aacgtcggct cgattgtacc tgcgttcaaa    15240

tactttgcga tcgtgttgcg cgcctgcccg gtgcgtcggc tgatctcacg gatcgactgc    15300

ttctctcgca acgccatccg acggatgatg tttaaaagtc ccatgtggat cactccgttg    15360

ccccgtcgct caccgtgttg gggggaaggt gcacatggct cagttctcaa tggaaattat    15420

ctgcctaacc ggctcagttc tgcgtagaaa ccaacatgca agctccaccg ggtgcaaagc    15480

ggcagcggc                                                            15489


<210>  68
<211>  21
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Single strand DNA oligonucleotide

<400>  68
gggcacagca caagtatatg g                                                 21


<210>  69
<211>  18
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Single strand DNA oligonucleotide

<400>  69
gcagtggggg aaattttg                                                     18


<210>  70
<211>  18
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Single strand DNA oligonucleotide

<400>  70
gcaaccaggg gagaagag                                                     18


<210>  71
<211>  18
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Single strand DNA oligonucleotide

<400>  71
aatcacccaa cccaatcg                                                     18


<210>  72
<211>  23
<212>  DNA
<213>  Arabidopsis thaliana

<400>  72
gacgtgataa gtctaagatc tgg                                               23


<210>  73
<211>  22
<212>  DNA
<213>  Arabidopsis thaliana

<400>  73
gcttttaata cgtaacgtag gg                                                22


<210>  74
<211>  20
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Single strand DNA oligonucleotide

<400>  74
tcaccaaagc cctaaagacg                                                   20


<210>  75
<211>  22
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Single strand DNA oligonucleotide

<400>  75
catgttggaa atgactttga cc                                                22


<210>  76
<211>  22
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Single strand DNA oligonucleotide

<400>  76
gcgactatag cgttatcctt tg                                                22


<210>  77
<211>  23
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Single strand DNA oligonucleotide

<400>  77
gcataaacaa actggttaac gtg                                               23


<210>  78
<211>  284
<212>  DNA
<213>  Arabidopsis thaliana

<400>  78
tgagaataag gagaagagat gaaaaagaga atcaagaata caagaaaggt ttatggacag       60

ttgaagaaga caacatcctt atggactatg ttcttaatca tggcactggc caatggaacc      120

gcatcgtcag aaaaactggt actctttctc tctctcgcgc gttttgtgat tatatatatt      180

aactttagtc tctagctagt tcatatataa atatatctca gttagactaa atttgattcg      240

ttgatagggc taaagagatg tgggaaaagt tgtagactga gatg                       284


<210>  79
<211>  254
<212>  DNA
<213>  Artificial sequence

<220>
<223>  sequencing results of GL-1 mutated region


<220>
<221>  misc_feature
<222>  (240)..(242)
<223>  n is a, c, g, or t

<220>
<221>  misc_feature
<222>  (245)..(245)
<223>  n is a, c, g, or t

<220>
<221>  misc_feature
<222>  (247)..(249)
<223>  n is a, c, g, or t

<220>
<221>  misc_feature
<222>  (251)..(254)
<223>  n is a, c, g, or t

<400>  79
ttgagaattg agaataagga gaagagataa aaacgagaat ccagaataca gaaaggacta       60

tggacagtgg aagaagccaa catccttatg gtctatgttc atggcactgg ccaatggaac      120

cgcatcgtca gaaaaactgg tactctttct ctctctcgcg cgttttgtga ttatatatat      180

taactttagt ctctagctag ttcatatata aatatatctc agttagacta aattgattcn      240

nnatngnnna nnnn                                                        254


