                         SEQUENCE LISTING

<110>  University of Bremen
 
<120>  Method of generating polyploid plants

<130>  UN15A18/P-WO

<150>  EP 16 179 967.1
<151>  2016-07-18

<160>  47    

<170>  PatentIn version 3.5

<210>  1
<211>  28
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  TN13s Forward (Fw) Primer (5' - 3') - for amplification of the 
       UAS promoter

<400>  1
atggcgcgcc gcatgcctgc aggtcgga                                          28


<210>  2
<211>  28
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  TN13as Reverse (Rev) Primer (5' - 3') - for amplification of the 
       UAS promoter

<400>  2
atttaattaa cggggatccg gttctctc                                          28


<210>  3
<211>  30
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  TN14s Forward (Fw) Primer (5' - 3') - for amplification of the 
       BAR gene

<400>  3
atttaattaa atgagcccag aacgacgccc                                        30


<210>  4
<211>  31
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  TN14as Reverse (Rev) Primer (5' - 3') for amplification of the 
       BAR gene

<400>  4
atgcggccgc gatttcggtg acgggcagga c                                      31


<210>  5
<211>  33
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  TN12s Forward (Fw) Primer (5' - 3') - for amplification of 
       GAL4:VP16

<400>  5
atttaattaa atgaagctcc tgtcctccat cga                                    33


<210>  6
<211>  33
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  TN12as Reverse (Rev) Primer (5' - 3') for amplification of 
       GAL4:VP16

<400>  6
atgcggccgc ctacccaccg tactcgtcaa ttc                                    33


<210>  7
<211>  30
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  TS15s Forward (Fw) Primer (5' - 3') - for amplification of the 
       PRPS5a promoter

<400>  7
aggcgcgccg ggccataatc gtgagtagat                                        30


<210>  8
<211>  27
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  TS15as Reverse (Rev) Primer (5' - 3') - for amplification of the 
       PRPS5a promoter

<400>  8
acgatcgcgg ctgtggtgag agaaaca                                           27


<210>  9
<211>  549
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  BAR gene amplfied from pGreenII 0229

<400>  9
atgagcccag aacgacgccc ggccgacatc cgccgtgcca ccgaggcgga catgccggcg       60

gtctgcacca tcgtcaacca ctacatcgag acaagcacgg tcaacttccg taccgagccg      120

caggaaccgc aggagtggac ggacgacctc gtccgtctgc gggagcgcta tccctggctc      180

gtcgccgagg tggacggcga ggtcgccggc atcgcctacg cgggtccctg gaaggcacgc      240

aacgcctacg actggacggc cgagtcgacc gtgtacgtct ccccccgcca ccagcggacg      300

ggactgggct ccacgctcta cacccacctg ctgaagtccc tggaggcaca gggcttcaag      360

agcgtggtcg ctgtcatcgg gctgcccaac gacccgagcg tgcgcatgca cgaggcgctc      420

ggatatgccc cccgcggcat gctgcgggcg gccggcttca agcacgggaa ctggcatgac      480

gtgggtttct ggcagctgga cttcagcctg ccggtgccgc cccgtccggt cctgcccgtc      540

accgaaatc                                                              549


<210>  10
<211>  188
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  UAS promoter

<400>  10
gcatgcctgc aggtcggagt actgtcctcc gagcggagta ctgtcctccg agcggagtac       60

tgtcctccga gcggagtact gtcctccgag cggagtactg tcctccgagc ggagactcta      120

gaggatcttc gcaagaccct tcctctatat aaggaagttc atttcatttg gagagaaccg      180

gatccccg                                                               188


<210>  11
<211>  1688
<212>  DNA
<213>  Arabidopsis thaliana

<400>  11
gggccataat cgtgagtaga tatattactc aacttttgat tcgctatttg cagtgcacct       60

gtggcgttca tcacatcttt tgtgacactg tttgcactgg tcattgctat tacaaaggac      120

cttcctgatg ttgaaggaga tcgaaagtaa gtaactgcac gcataaccat tttctttccg      180

ctctttggct caatccattt gacagtcaaa gacaatgttt aaccagctcc gtttgatata      240

ttgtctttat gtgtttgttc aagcatgttt agttaatcat gcctttgatt gatcttgaat      300

aggttccaaa tatcaaccct ggcaacaaaa cttggagtga gaaacattgc attcctcggt      360

tctggacttc tgctagtaaa ttatgtttca gccatatcac tagctttcta catgcctcag      420

gtgaattcat ctatttccgt cttaactatt tcggttaatt aaagcacgaa caccattact      480

gcatgtagaa gcttgataaa ctatcgccac caatttattt ttgttgcgat attgttactt      540

tcctcagtat gcagctttga aaagaccaac cctcttatcc tttaacaatg aacaggtttt      600

tagaggtagc ttgatgattc ctgcacatgt gatcttggct tcaggcttaa ttttccaggt      660

aaagcattat gagatactct tatatctctt acatactttt gagataatgc acaagaactt      720

cataactata tgctttagtt tctgcatttg acactgccaa attcattaat ctctaatatc      780

tttgttgttg atctttggta gacatgggta ctagaaaaag caaactacac caaggtaaaa      840

tacttttgta caaacataaa ctcgttatca cggaacatca atggagtgta tatctaacgg      900

agtgtagaaa catttgatta ttgcaggaag ctatctcagg atattatcgg tttatatgga      960

atctcttcta cgcagagtat ctgttattcc ccttcctcta gctttcaatt tcatggtgag     1020

gatatgcagt tttctttgta tatcattctt cttcttcttt gtagcttgga gtcaaaatcg     1080

gttccttcat gtacatacat caaggatatg tccttctgaa tttttatatc ttgcaataaa     1140

aatgcttgta ccaattgaaa caccagcttt ttgagttcta tgatcactga cttggttcta     1200

accaaaaaaa aaaaaatgtt taatttacat atctaaaagt aggtttaggg aaacctaaac     1260

agtaaaatat ttgtatatta ttcgaatttc actcatcata aaaacttaaa ttgcaccata     1320

aaattttgtt ttactattaa tgatgtaatt tgtgtaactt aagataaaaa taatattccg     1380

taagttaacc ggctaaaacc acgtataaac cagggaacct gttaaaccgg ttctttactg     1440

gataaagaaa tgaaagccca tgtagacagc tccattagag cccaaaccct aaatttctca     1500

tctatataaa aggagtgaca ttagggtttt tgttcgtcct cttaaagctt ctcgttttct     1560

ctgccgtctc tctcattcgc gcgacgcaaa cgatcttcag gtgatcttct ttctccaaat     1620

cctctctcat aactctgatt tcgtacttgt gtatttgagc tcacgctctg tttctctcac     1680

cacagccg                                                              1688


<210>  12
<211>  716
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  YFP gene

<400>  12
tgtgagcaag ggcgaggagc tgttcaccgg ggtggtgccc atcctggtcg agctggacgg       60

cgacgtaaac ggccacaagt tcagcgtgtc cggcgagggc gagggcgatg ccacctacgg      120

caagctgacc ctgaagttca tctgcaccac cggcaagctg cccgtgccct ggcccaccct      180

cgtgaccacc ttcggctacg gcctgcagtg cttcgcccgc taccccgacc acatgaagca      240

gcacgacttc ttcaagtccg ccatgcccga aggctacgtc caggagcgca ccatcttctt      300

caaggacgac ggcaactaca agacccgcgc cgaggtgaag ttcgagggcg acaccctggt      360

gaaccgcatc gagctgaagg gcatcgactt caaggaggac ggcaacatcc tggggcacaa      420

gctggagtac aactacaaca gccacaacgt ctatatcatg gccgacaagc agaagaacgg      480

catcaaggtg aacttcaaga tccgccacaa catcgaggac ggcagcgtgc agctcgccga      540

ccactaccag cagaacaccc ccatcggcga cggccccgtg ctgctgcccg acaaccacta      600

cctgagctac cagtccgccc tgagcaaaga ccccaacgag aagcgcgatc acatggtcct      660

gctggagttc gtgaccgccg ccgggatcac tctcggcatg gacgagctgt acaagt          716


<210>  13
<211>  681
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  GAL4:VP16 construct

<400>  13
tgaagctcct gtcctccatc gagcaggcct gcgacatctg ccgcctcaag aagctcaagt       60

gctccaagaa gaagccgaag tgcgccaagt gtctgaagaa caactgggag tgtcgctact      120

ctcccaaaac caagcgctcc ccgctgaccc gcgcccacct caccgaagtg gagtcccgcc      180

tggagcgcct ggagcagctc ttcctcctga tcttccctcg agaggacctc gacatgatcc      240

tgaaaatgga ctccctccag gacatcaaag ccctgctcac cggcctcttc gtccaggaca      300

acgtgaacaa agacgccgtc accgaccgcc tggcctccgt ggagaccgac atgcccctca      360

ccctgcgcca gcaccgcatc agcgcgacct cctcctcgga ggagagcagc aacaagggcc      420

agcgccagtt gaccgtctcg acggcccccc cgaccgacgt cagcctgggg gacgagctcc      480

acttagacgg cgaggacgtg gcgatggcgc atgccgacgc gctagacgat ttcgatctgg      540

acatgttggg ggacggggat tccccggggc cgggatttac cccccacgac tccgccccct      600

acggcgctct ggatacggcc gacttcgagt ttgagcagat gtttaccgat gcccttggaa      660

ttgacgagta cggtgggtag c                                                681


<210>  14
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Forward (Fw) Primer (5' - 3') targeting the PD2 construct 
       (UAS::BAR_YFP)

<400>  14
tatagggcga attgggtacc                                                   20


<210>  15
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Reverse (Rev) Primer (5' - 3') targeting the PD2 construct 
       (UAS::BAR_YFP)

<400>  15
ggaactggca tgacgtgggt tt                                                22


<210>  16
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Forward (Fw) Primer (5' - 3') targeting the PD1 construct 
       (pRPS5a::mGAL4-VP16)

<400>  16
tcgttttctc tgccgtctct ct                                                22


<210>  17
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Reverse (Rev) Primer (5' - 3') targeting the PD1 construct 
       (pRPS5a::mGAL4-VP16)

<400>  17
cccttgttgc tgctctcctc                                                   20


<210>  18
<211>  28
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  DT51 Forward (Fw) Primer (5' - 3')

<400>  18
caggacatcc acctagaaac aagaagac                                          28


<210>  19
<211>  28
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  DT52 Reverse (Rev) Primer (5' - 3')

<400>  19
tgaatgtgtg cagatcatgg agaaggac                                          28


<210>  20
<211>  24
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  DT53 Forward (Fw) Primer (5' - 3')

<400>  20
tgtacctgga ttcgatagaa ctgc                                              24


<210>  21
<211>  24
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  DT54 Reverse (Rev) Primer (5' - 3')

<400>  21
agaagtccaa gcatgtccta tggt                                              24


<210>  22
<211>  25
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  DT55 Forward (Fw) Primer (5' - 3')

<400>  22
taagattgct cagaagctga gtgag                                             25


<210>  23
<211>  25
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  DT56 Reverse (Rev) Primer (5' - 3')

<400>  23
cgacacacaa tgtatctgtt taccc                                             25


<210>  24
<211>  27
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  DT92 Forward (Fw) Primer (5' - 3')

<400>  24
acctctaaac gtgattgcaa ctgtgag                                           27


<210>  25
<211>  27
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  DT93 Reverse (Rev) Primer (5' - 3')

<400>  25
tgtgccaaca atctctcatg tacatgg                                           27


<210>  26
<211>  27
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  DT59 Forward (Fw) Primer (5' - 3')

<400>  26
tggtcgttgc ttaagttgtt aagctcg                                           27


<210>  27
<211>  28
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  DT60 Reverse (Rev) Primer (5' - 3')

<400>  27
caatacaaga cactttctcc aatatggg                                          28


<210>  28
<211>  27
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  DT61 Forward (Fw) Primer (5' - 3')

<400>  28
tggttcacat gtacgccaag tgttacc                                           27


<210>  29
<211>  27
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  DT89 Reverse (Rev) Primer (5' - 3')

<400>  29
cttcccttca ttaaccatcc ctgtgtg                                           27


<210>  30
<211>  27
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  DT116 Forward (Fw) Primer (5' - 3')

<400>  30
ttatttccga ctttgtcttt gccatcg                                           27


<210>  31
<211>  27
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  DT117 Reverse (Rev) Primer (5' - 3')

<400>  31
tgctagatta tgcctttgat cacaagc                                           27


<210>  32
<211>  27
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  DT65 Forward (Fw) Primer (5' - 3')

<400>  32
gctgcaatgg taaacaagga ggaagag                                           27


<210>  33
<211>  27
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  DT66 Reverse (Rev) Primer (5' - 3')

<400>  33
cgggatcata atgccgatat tcttcgg                                           27


<210>  34
<211>  27
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  DT67 Forward (Fw) Primer (5' - 3')

<400>  34
tgtcttcagc atatatgccg attggag                                           27


<210>  35
<211>  27
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  DT68 Reverse (Rev) Primer (5' - 3')

<400>  35
caatctcttc aggtgagata atgagcg                                           27


<210>  36
<211>  27
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  DT96 Forward (Fw) Primer (5' - 3')

<400>  36
acacgcaaga gagaattgga tgatacc                                           27


<210>  37
<211>  27
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  DT97 Reverse (Rev) Primer (5' - 3')

<400>  37
agcagaatct caatctcgat ggttgtg                                           27


<210>  38
<211>  27
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  DT71 Forward (Fw) Primer (5' - 3')

<400>  38
tctcttgctc tcttctctct cacacag                                           27


<210>  39
<211>  27
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  DT72 Reverse (Rev) Primer (5' - 3')

<400>  39
acctcttgtg gagtatcaag ccaagtg                                           27


<210>  40
<211>  27
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  DT98 Forward (Fw) Primer (5' - 3')

<400>  40
tacagagtcc aagtcgttgt catgctc                                           27


<210>  41
<211>  27
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  DT99 Reverse (Rev) Primer (5' - 3')

<400>  41
tgcgtgactg ctatatccta aaggagg                                           27


<210>  42
<211>  25
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  DT77 Forward (Fw) Primer (5' - 3')

<400>  42
caacgagaag ttggattgtg aggtg                                             25


<210>  43
<211>  25
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  DT78 Reverse (Rev) Primer (5' - 3')

<400>  43
ggatgaagga gcatatggag atagc                                             25


<210>  44
<211>  27
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  DT79 Forward (Fw) Primer (5' - 3')

<400>  44
gtattcacga ccatcgtagc tgtccac                                           27


<210>  45
<211>  27
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  DT80 Reverse (Rev) Primer (5' - 3')

<400>  45
aactcgcctc tgaagctggt caatgag                                           27


<210>  46
<211>  27
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  DT81 Forward (Fw) Primer (5' - 3')

<400>  46
aacagtaatg gtccccattc acggtgg                                           27


<210>  47
<211>  27
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  DT82 Reverse (Rev) Primer (5' - 3')

<400>  47
ccaattagac cttgaaggag caggacc                                           27


