                         SEQUENCE LISTING

<110>  F. Hoffmann-La Roche AG
 
<120>  METHOD AND MEANS FOR DETECTING THE LEVEL OF TOTAL VEGF-A

<130>  P33749-WO

<150>  EP16179780.8
<151>  2016-07-15

<160>  5     

<170>  PatentIn version 3.5

<210>  1
<211>  232
<212>  PRT
<213>  Homo sapiens

<400>  1

Met Asn Phe Leu Leu Ser Trp Val His Trp Ser Leu Ala Leu Leu Leu 
1               5                   10                  15      


Tyr Leu His His Ala Lys Trp Ser Gln Ala Ala Pro Met Ala Glu Gly 
            20                  25                  30          


Gly Gly Gln Asn His His Glu Val Val Lys Phe Met Asp Val Tyr Gln 
        35                  40                  45              


Arg Ser Tyr Cys His Pro Ile Glu Thr Leu Val Asp Ile Phe Gln Glu 
    50                  55                  60                  


Tyr Pro Asp Glu Ile Glu Tyr Ile Phe Lys Pro Ser Cys Val Pro Leu 
65                  70                  75                  80  


Met Arg Cys Gly Gly Cys Cys Asn Asp Glu Gly Leu Glu Cys Val Pro 
                85                  90                  95      


Thr Glu Glu Ser Asn Ile Thr Met Gln Ile Met Arg Ile Lys Pro His 
            100                 105                 110         


Gln Gly Gln His Ile Gly Glu Met Ser Phe Leu Gln His Asn Lys Cys 
        115                 120                 125             


Glu Cys Arg Pro Lys Lys Asp Arg Ala Arg Gln Glu Lys Lys Ser Val 
    130                 135                 140                 


Arg Gly Lys Gly Lys Gly Gln Lys Arg Lys Arg Lys Lys Ser Arg Tyr 
145                 150                 155                 160 


Lys Ser Trp Ser Val Tyr Val Gly Ala Arg Cys Cys Leu Met Pro Trp 
                165                 170                 175     


Ser Leu Pro Gly Pro His Pro Cys Gly Pro Cys Ser Glu Arg Arg Lys 
            180                 185                 190         


His Leu Phe Val Gln Asp Pro Gln Thr Cys Lys Cys Ser Cys Lys Asn 
        195                 200                 205             


Thr Asp Ser Arg Cys Lys Ala Arg Gln Leu Glu Leu Asn Glu Arg Thr 
    210                 215                 220                 


Cys Arg Cys Asp Lys Pro Arg Arg 
225                 230         


<210>  2
<211>  233
<212>  PRT
<213>  homo sapiens

<400>  2

Met Gly Val Pro Thr Gln Leu Leu Leu Leu Gly Leu Thr Val Val Val 
1               5                   10                  15      


Val Arg Cys Asp Ile Gln Met Thr Gln Ser Pro Ala Ser Leu Ser Ala 
            20                  25                  30          


Ser Val Gly Glu Thr Val Thr Ile Thr Cys Gly Ala Ser Glu Asn Ile 
        35                  40                  45              


Tyr Gly Gly Leu Thr Trp Tyr Gln Arg Arg Arg Gly Lys Ser Pro Gln 
    50                  55                  60                  


Leu Leu Ile Tyr Gly Ala Ser Asn Leu Ala Asp Gly Met Ser Ser Arg 
65                  70                  75                  80  


Phe Thr Gly Ser Gly Ser Gly Arg Gln Phe Ser Leu Lys Ile Ser Gly 
                85                  90                  95      


Val His Pro Asp Asp Val Ala Thr Tyr Tyr Cys Gln Asn Val Phe Thr 
            100                 105                 110         


Thr Pro Tyr Thr Phe Gly Gly Gly Thr Arg Leu Glu Ile Lys Arg Ala 
        115                 120                 125             


Asp Ala Ala Pro Thr Val Ser Ile Phe Pro Pro Ser Ser Glu Gln Leu 
    130                 135                 140                 


Thr Ser Gly Gly Ala Ser Val Val Cys Phe Leu Asn Asn Phe Tyr Pro 
145                 150                 155                 160 


Lys Asp Ile Asn Val Lys Trp Lys Ile Asp Gly Ser Glu Arg Gln Asn 
                165                 170                 175     


Gly Val Leu Asn Ser Trp Thr Asp Gln Asp Ser Lys Asp Ser Thr Tyr 
            180                 185                 190         


Ser Met Ser Ser Thr Leu Thr Leu Thr Lys Asp Glu Tyr Glu Arg His 
        195                 200                 205             


Asn Ser Tyr Thr Cys Glu Ala Thr His Lys Thr Ser Thr Ser Pro Ile 
    210                 215                 220                 


Val Lys Ser Phe Asn Arg Asn Glu Cys 
225                 230             


<210>  3
<211>  472
<212>  PRT
<213>  Homo sapiens

<400>  3

Met Arg Val Leu Ile Leu Leu Cys Leu Phe Thr Val Phe Pro Gly Ile 
1               5                   10                  15      


Leu Ser Asp Val Arg Leu Gln Glu Ser Gly Pro Asp Leu Val Lys Pro 
            20                  25                  30          


Ser Gln Ser Leu Ser Leu Thr Cys Thr Val Thr Gly Tyr Ser Ile Thr 
        35                  40                  45              


Arg Ala Tyr Asn Trp His Trp Ile Arg Gln Phe Pro Gly Asn Lys Leu 
    50                  55                  60                  


Glu Trp Met Gly Tyr Ile Leu Tyr Ser Gly Thr Thr Asp Tyr Asn Pro 
65                  70                  75                  80  


Ser Leu Lys Ser Arg Ile Ser Phe Thr Arg Asp Thr Ser Lys Asn Gln 
                85                  90                  95      


Leu Ile Leu Gln Leu Asp Ser Val Thr Thr Asp Asp Thr Gly Thr Tyr 
            100                 105                 110         


Tyr Cys Ala Arg Ala Gly Tyr Asp Gly Gly Met Asp Tyr Trp Gly Gln 
        115                 120                 125             


Gly Val Ser Val Thr Val Ser Ser Ala Lys Thr Thr Pro Pro Ser Val 
    130                 135                 140                 


Tyr Pro Leu Ala Pro Gly Cys Gly Asp Thr Thr Gly Ser Ser Val Thr 
145                 150                 155                 160 


Leu Gly Cys Leu Val Lys Gly Tyr Phe Pro Glu Ser Val Thr Val Thr 
                165                 170                 175     


Trp Asn Ser Gly Ser Leu Ser Ser Ser Val His Thr Phe Pro Ala Leu 
            180                 185                 190         


Leu Gln Ser Gly Leu Tyr Thr Met Ser Ser Ser Val Thr Val Pro Ser 
        195                 200                 205             


Ser Thr Trp Pro Ser Gln Thr Val Thr Cys Ser Val Ala His Pro Ala 
    210                 215                 220                 


Ser Ser Thr Thr Val Asp Lys Lys Leu Glu Pro Ser Gly Pro Ile Ser 
225                 230                 235                 240 


Thr Ile Asn Pro Cys Pro Pro Cys Lys Glu Cys His Lys Cys Pro Ala 
                245                 250                 255     


Pro Asn Leu Glu Gly Gly Pro Ser Val Phe Ile Phe Pro Pro Asn Ile 
            260                 265                 270         


Lys Asp Val Leu Met Ile Ser Leu Thr Pro Lys Val Thr Cys Val Val 
        275                 280                 285             


Val Asp Val Ser Glu Asp Asp Pro Asp Val Gln Ile Ser Trp Phe Val 
    290                 295                 300                 


Asn Asn Val Glu Val His Thr Ala Gln Thr Gln Thr His Arg Glu Asp 
305                 310                 315                 320 


Tyr Asn Ser Thr Ile Arg Val Val Ser Thr Leu Pro Ile Gln His Gln 
                325                 330                 335     


Asp Trp Met Ser Gly Lys Glu Phe Lys Cys Lys Val Asn Asn Lys Asp 
            340                 345                 350         


Leu Pro Ser Pro Ile Glu Arg Thr Ile Ser Lys Ile Lys Gly Leu Val 
        355                 360                 365             


Arg Ala Pro Gln Val Tyr Ile Leu Pro Pro Pro Ala Glu Gln Leu Ser 
    370                 375                 380                 


Arg Lys Asp Val Ser Leu Thr Cys Leu Val Val Gly Phe Asn Pro Gly 
385                 390                 395                 400 


Asp Ile Ser Val Glu Trp Thr Ser Asn Gly His Thr Glu Glu Asn Tyr 
                405                 410                 415     


Lys Asp Thr Ala Pro Val Leu Asp Ser Asp Gly Ser Tyr Phe Ile Tyr 
            420                 425                 430         


Ser Lys Leu Asn Met Lys Thr Ser Lys Trp Glu Lys Thr Asp Ser Phe 
        435                 440                 445             


Ser Cys Asn Val Arg His Glu Gly Leu Lys Asn Tyr Tyr Leu Lys Lys 
    450                 455                 460                 


Thr Ile Ser Arg Ser Pro Gly Lys 
465                 470         


<210>  4
<211>  234
<212>  PRT
<213>  Homo sapiens

<400>  4

Met Lys Ser Gln Thr Gln Val Phe Val Phe Leu Leu Leu Cys Val Ser 
1               5                   10                  15      


Gly Val His Gly Ser Ile Val Met Thr Gln Thr Pro Lys Phe Leu Leu 
            20                  25                  30          


Val Ser Pro Gly Asp Arg Val Thr Ile Thr Cys Lys Ala Ser Gln Ser 
        35                  40                  45              


Val Gly His Asp Val Ser Trp Tyr Arg Gln Lys Pro Gly Gln Ser Pro 
    50                  55                  60                  


Lys Val Leu Ile Tyr Tyr Ala Ser Asn Arg Tyr Thr Gly Val Pro Asp 
65                  70                  75                  80  


Arg Phe Thr Gly Ser Gly Tyr Gly Thr Asp Phe Thr Phe Thr Val Arg 
                85                  90                  95      


Ser Val Arg Thr Glu Asp Leu Ala Val Tyr Phe Cys Gln Gln Asp Tyr 
            100                 105                 110         


Thr Ser Pro Leu Thr Phe Gly Thr Gly Thr Lys Leu Glu Leu Lys Arg 
        115                 120                 125             


Ala Asp Ala Ala Pro Thr Val Ser Ile Phe Pro Pro Ser Ser Glu Gln 
    130                 135                 140                 


Leu Thr Ser Gly Gly Ala Ser Val Val Cys Phe Leu Asn Asn Phe Tyr 
145                 150                 155                 160 


Pro Lys Asp Ile Asn Val Lys Trp Lys Ile Asp Gly Ser Glu Arg Gln 
                165                 170                 175     


Asn Gly Val Leu Asn Ser Trp Thr Asp Gln Asp Ser Lys Asp Ser Thr 
            180                 185                 190         


Tyr Ser Met Ser Ser Thr Leu Thr Leu Thr Lys Asp Glu Tyr Glu Arg 
        195                 200                 205             


His Asn Ser Tyr Thr Cys Glu Ala Thr His Lys Thr Ser Thr Ser Pro 
    210                 215                 220                 


Ile Val Lys Ser Phe Asn Arg Asn Glu Cys 
225                 230                 


<210>  5
<211>  473
<212>  PRT
<213>  Homo sapiens

<400>  5

Met Gly Trp Asn Tyr Ile Ile Leu Phe Leu Val Thr Thr Ala Thr Asp 
1               5                   10                  15      


Val His Ser Gln Ala Gln Leu Gln Gln Pro Gly Ala Glu Leu Val Lys 
            20                  25                  30          


Pro Gly Ala Ser Val Asn Leu Ser Cys Lys Ala Ser Gly Tyr Thr Phe 
        35                  40                  45              


Thr Asn Tyr Trp Ile His Trp Val Ser Gln Arg Pro Gly Gln Gly Leu 
    50                  55                  60                  


Glu Trp Ile Gly Glu Ile Asn Pro Ser Asp Gly Arg Thr Tyr Tyr Asn 
65                  70                  75                  80  


Glu Lys Phe Lys Asn Lys Val Ile Leu Thr Val Asp Glu Ala Ser Thr 
                85                  90                  95      


Thr Ala Tyr Leu Gln Leu Ser Ser Leu Thr Ser Glu Asp Ser Ala Val 
            100                 105                 110         


Tyr Tyr Cys Thr Ser Arg Tyr Tyr Ile Tyr Gly Val Asp Tyr Trp Gly 
        115                 120                 125             


Gln Gly Thr Thr Leu Thr Val Ser Ser Ala Lys Thr Thr Pro Pro Ser 
    130                 135                 140                 


Val Tyr Pro Leu Ala Pro Gly Cys Gly Asp Thr Thr Gly Ser Ser Val 
145                 150                 155                 160 


Thr Leu Gly Cys Leu Val Lys Gly Tyr Phe Pro Glu Ser Val Thr Val 
                165                 170                 175     


Thr Trp Asn Ser Gly Ser Leu Ser Ser Ser Val His Thr Phe Pro Ala 
            180                 185                 190         


Leu Leu Gln Ser Gly Leu Tyr Thr Met Ser Ser Ser Val Thr Val Pro 
        195                 200                 205             


Ser Ser Thr Trp Pro Ser Gln Thr Val Thr Cys Ser Val Ala His Pro 
    210                 215                 220                 


Ala Ser Ser Thr Thr Val Asp Lys Lys Leu Glu Pro Ser Gly Pro Ile 
225                 230                 235                 240 


Ser Thr Ile Asn Pro Cys Pro Pro Cys Lys Glu Cys His Lys Cys Pro 
                245                 250                 255     


Ala Pro Asn Leu Glu Gly Gly Pro Ser Val Phe Ile Phe Pro Pro Asn 
            260                 265                 270         


Ile Lys Asp Val Leu Met Ile Ser Leu Thr Pro Lys Val Thr Cys Val 
        275                 280                 285             


Val Val Asp Val Ser Glu Asp Asp Pro Asp Val Gln Ile Gly Trp Phe 
    290                 295                 300                 


Val Asn Asn Val Glu Val His Thr Ala Gln Thr Gln Thr His Arg Glu 
305                 310                 315                 320 


Asp Tyr Asn Ser Thr Ile Arg Val Val Ser Thr Leu Pro Ile Gln His 
                325                 330                 335     


Gln Asp Trp Met Ser Gly Lys Glu Phe Lys Cys Lys Val Asn Asn Lys 
            340                 345                 350         


Asp Leu Pro Ser Pro Ile Glu Arg Thr Ile Ser Lys Ile Lys Gly Leu 
        355                 360                 365             


Val Arg Ala Pro Gln Val Tyr Ile Leu Pro Pro Pro Ala Glu Gln Leu 
    370                 375                 380                 


Ser Arg Lys Asp Val Ser Leu Thr Cys Leu Val Val Gly Phe Asn Pro 
385                 390                 395                 400 


Gly Asp Ile Ser Val Glu Trp Thr Ser Asn Gly His Thr Glu Glu Asn 
                405                 410                 415     


Tyr Lys Asp Thr Ala Pro Val Leu Asp Ser Asp Gly Ser Tyr Phe Ile 
            420                 425                 430         


Tyr Ser Lys Leu Asn Met Lys Thr Ser Lys Trp Glu Lys Thr Asp Ser 
        435                 440                 445             


Phe Ser Cys Asn Val Arg His Glu Gly Leu Lys Asn Tyr Tyr Leu Lys 
    450                 455                 460                 


Lys Thr Ile Ser Arg Ser Pro Gly Lys 
465                 470             


