                         SEQUENCE LISTING

<110>  Wageningen Universiteit
 
<120>  Prokaryotic Argonaute proteins and uses thereof

<130>  P234172WO

<150>  GB1612090.9
<151>  2016-07-12

<150>  GB1619551.3
<151>  2016-11-18

<160>  9     

<170>  PatentIn version 3.5

<210>  1
<211>  748
<212>  PRT
<213>  Clostridium butyricum

<400>  1

Met Asn Asn Leu Thr Phe Glu Ala Phe Glu Gly Ile Gly Gln Leu Asn 
1               5                   10                  15      


Glu Leu Asn Phe Tyr Lys Tyr Arg Leu Ile Gly Lys Gly Gln Ile Asp 
            20                  25                  30          


Asn Val His Gln Ala Ile Trp Ser Val Lys Tyr Lys Leu Gln Ala Asn 
        35                  40                  45              


Asn Phe Phe Lys Pro Val Phe Val Lys Gly Glu Ile Leu Tyr Ser Leu 
    50                  55                  60                  


Asp Glu Leu Lys Val Ile Pro Glu Phe Glu Asn Val Glu Val Ile Leu 
65                  70                  75                  80  


Asp Gly Asn Ile Ile Leu Ser Ile Ser Glu Asn Thr Asp Ile Tyr Lys 
                85                  90                  95      


Asp Val Ile Val Phe Tyr Ile Asn Asn Ala Leu Lys Asn Ile Lys Asp 
            100                 105                 110         


Ile Thr Asn Tyr Arg Lys Tyr Ile Thr Lys Asn Thr Asp Glu Ile Ile 
        115                 120                 125             


Cys Lys Ser Ile Leu Thr Thr Asn Leu Lys Tyr Gln Tyr Met Lys Ser 
    130                 135                 140                 


Glu Lys Gly Phe Lys Leu Gln Arg Lys Phe Lys Ile Ser Pro Val Val 
145                 150                 155                 160 


Phe Arg Asn Gly Lys Val Ile Leu Tyr Leu Asn Cys Ser Ser Asp Phe 
                165                 170                 175     


Ser Thr Asp Lys Ser Ile Tyr Glu Met Leu Asn Asp Gly Leu Gly Val 
            180                 185                 190         


Val Gly Leu Gln Val Lys Asn Lys Trp Thr Asn Ala Asn Gly Asn Ile 
        195                 200                 205             


Phe Ile Glu Lys Val Leu Asp Asn Thr Ile Ser Asp Pro Gly Thr Ser 
    210                 215                 220                 


Gly Lys Leu Gly Gln Ser Leu Ile Asp Tyr Tyr Ile Asn Gly Asn Gln 
225                 230                 235                 240 


Lys Tyr Arg Val Glu Lys Phe Thr Asp Glu Asp Lys Asn Ala Lys Val 
                245                 250                 255     


Ile Gln Ala Lys Ile Lys Asn Lys Thr Tyr Asn Tyr Ile Pro Gln Ala 
            260                 265                 270         


Leu Thr Pro Val Ile Thr Arg Glu Tyr Leu Ser His Thr Asp Lys Lys 
        275                 280                 285             


Phe Ser Lys Gln Ile Glu Asn Val Ile Lys Met Asp Met Asn Tyr Arg 
    290                 295                 300                 


Tyr Gln Thr Leu Lys Ser Phe Val Glu Asp Ile Gly Val Ile Lys Glu 
305                 310                 315                 320 


Leu Asn Asn Leu His Phe Lys Asn Gln Tyr Tyr Thr Asn Phe Asp Phe 
                325                 330                 335     


Met Gly Phe Glu Ser Gly Val Leu Glu Glu Pro Val Leu Met Gly Ala 
            340                 345                 350         


Asn Gly Lys Ile Lys Asp Lys Lys Gln Ile Phe Ile Asn Gly Phe Phe 
        355                 360                 365             


Lys Asn Pro Lys Glu Asn Val Lys Phe Gly Val Leu Tyr Pro Glu Gly 
    370                 375                 380                 


Cys Met Glu Asn Ala Gln Ser Ile Ala Arg Ser Ile Leu Asp Phe Ala 
385                 390                 395                 400 


Thr Ala Gly Lys Tyr Asn Lys Gln Glu Asn Lys Tyr Ile Ser Lys Asn 
                405                 410                 415     


Leu Met Asn Ile Gly Phe Lys Pro Ser Glu Cys Ile Phe Glu Ser Tyr 
            420                 425                 430         


Lys Leu Gly Asp Ile Thr Glu Tyr Lys Ala Thr Ala Arg Lys Leu Lys 
        435                 440                 445             


Glu His Glu Lys Val Gly Phe Val Ile Ala Val Ile Pro Asp Met Asn 
    450                 455                 460                 


Glu Leu Glu Val Glu Asn Pro Tyr Asn Pro Phe Lys Lys Val Trp Ala 
465                 470                 475                 480 


Lys Leu Asn Ile Pro Ser Gln Met Ile Thr Leu Lys Thr Thr Glu Lys 
                485                 490                 495     


Phe Lys Asn Ile Val Asp Lys Ser Gly Leu Tyr Tyr Leu His Asn Ile 
            500                 505                 510         


Ala Leu Asn Ile Leu Gly Lys Ile Gly Gly Ile Pro Trp Ile Ile Lys 
        515                 520                 525             


Asp Met Pro Gly Asn Ile Asp Cys Phe Ile Gly Leu Asp Val Gly Thr 
    530                 535                 540                 


Arg Glu Lys Gly Ile His Phe Pro Ala Cys Ser Val Leu Phe Asp Lys 
545                 550                 555                 560 


Tyr Gly Lys Leu Ile Asn Tyr Tyr Lys Pro Thr Ile Pro Gln Ser Gly 
                565                 570                 575     


Glu Lys Ile Ala Glu Thr Ile Leu Gln Glu Ile Phe Asp Asn Val Leu 
            580                 585                 590         


Ile Ser Tyr Lys Glu Glu Asn Gly Glu Tyr Pro Lys Asn Ile Val Ile 
        595                 600                 605             


His Arg Asp Gly Phe Ser Arg Glu Asn Ile Asp Trp Tyr Lys Glu Tyr 
    610                 615                 620                 


Phe Asp Lys Lys Gly Ile Lys Phe Asn Ile Ile Glu Val Lys Lys Asn 
625                 630                 635                 640 


Ile Pro Val Lys Ile Ala Lys Val Val Gly Ser Asn Ile Cys Asn Pro 
                645                 650                 655     


Ile Lys Gly Ser Tyr Val Leu Lys Asn Asp Lys Ala Phe Ile Val Thr 
            660                 665                 670         


Thr Asp Ile Lys Asp Gly Val Ala Ser Pro Asn Pro Leu Lys Ile Glu 
        675                 680                 685             


Lys Thr Tyr Gly Asp Val Glu Met Lys Ser Ile Leu Glu Gln Ile Tyr 
    690                 695                 700                 


Ser Leu Ser Gln Ile His Val Gly Ser Thr Lys Ser Leu Arg Leu Pro 
705                 710                 715                 720 


Ile Thr Thr Gly Tyr Ala Asp Lys Ile Cys Lys Ala Ile Glu Tyr Ile 
                725                 730                 735     


Pro Gln Gly Val Val Asp Asn Arg Leu Phe Phe Leu 
            740                 745             


<210>  2
<211>  149
<212>  PRT
<213>  Clostridium butyricum

<400>  2

Met Thr Val Ile Asp Leu Asp Ser Thr Thr Thr Ala Asp Glu Leu Thr 
1               5                   10                  15      


Ser Gly His Thr Tyr Asp Ile Ser Val Thr Leu Thr Gly Val Tyr Asp 
            20                  25                  30          


Asn Thr Asp Glu Gln His Pro Arg Met Ser Leu Ala Phe Glu Gln Asp 
        35                  40                  45              


Asn Gly Glu Arg Arg Tyr Ile Thr Leu Trp Lys Asn Thr Thr Pro Lys 
    50                  55                  60                  


Asp Val Phe Thr Tyr Asp Tyr Ala Thr Gly Ser Thr Tyr Ile Phe Thr 
65                  70                  75                  80  


Asn Ile Asp Tyr Glu Val Lys Asp Gly Tyr Glu Asn Leu Thr Ala Thr 
                85                  90                  95      


Tyr Gln Thr Thr Val Glu Asn Ala Thr Ala Gln Glu Val Gly Thr Thr 
            100                 105                 110         


Asp Glu Asp Glu Thr Phe Ala Gly Gly Glu Pro Leu Asp His His Leu 
        115                 120                 125             


Asp Asp Ala Leu Asn Glu Thr Pro Asp Asp Ala Glu Thr Glu Ser Asp 
    130                 135                 140                 


Ser Gly His Val Met 
145                 


<210>  3
<211>  221
<212>  PRT
<213>  Clostridium butyricum

<400>  3

Lys Asp Met Pro Gly Asn Ile Asp Cys Phe Ile Gly Leu Asp Val Gly 
1               5                   10                  15      


Thr Arg Glu Lys Gly Ile His Phe Pro Ala Cys Ser Val Leu Phe Asp 
            20                  25                  30          


Lys Tyr Gly Lys Leu Ile Asn Tyr Tyr Lys Pro Thr Ile Pro Gln Ser 
        35                  40                  45              


Gly Glu Lys Ile Ala Glu Thr Ile Leu Gln Glu Ile Phe Asp Asn Val 
    50                  55                  60                  


Leu Ile Ser Tyr Lys Glu Glu Asn Gly Glu Tyr Pro Lys Asn Ile Val 
65                  70                  75                  80  


Ile His Arg Asp Gly Phe Ser Arg Glu Asn Ile Asp Trp Tyr Lys Glu 
                85                  90                  95      


Tyr Phe Asp Lys Lys Gly Ile Lys Phe Asn Ile Ile Glu Val Lys Lys 
            100                 105                 110         


Asn Ile Pro Val Lys Ile Ala Lys Val Val Gly Ser Asn Ile Cys Asn 
        115                 120                 125             


Pro Ile Lys Gly Ser Tyr Val Leu Lys Asn Asp Lys Ala Phe Ile Val 
    130                 135                 140                 


Thr Thr Asp Ile Lys Asp Gly Val Ala Ser Pro Asn Pro Leu Lys Ile 
145                 150                 155                 160 


Glu Lys Thr Tyr Gly Asp Val Glu Met Lys Ser Ile Leu Glu Gln Ile 
                165                 170                 175     


Tyr Ser Leu Ser Gln Ile His Val Gly Ser Thr Lys Ser Leu Arg Leu 
            180                 185                 190         


Pro Ile Thr Thr Gly Tyr Ala Asp Lys Ile Cys Lys Ala Ile Glu Tyr 
        195                 200                 205             


Ile Pro Gln Gly Val Val Asp Asn Arg Leu Phe Phe Leu 
    210                 215                 220     


<210>  4
<211>  896
<212>  PRT
<213>  Natronobacterium gregoryi

<400>  4

Met Val Pro Lys Lys Lys Arg Lys Val Ala Thr Val Ile Asp Leu Asp 
1               5                   10                  15      


Ser Thr Thr Thr Ala Asp Glu Leu Thr Ser Gly His Thr Tyr Asp Ile 
            20                  25                  30          


Ser Val Thr Leu Thr Gly Val Tyr Asp Asn Thr Asp Glu Gln His Pro 
        35                  40                  45              


Arg Met Ser Leu Ala Phe Glu Gln Asp Asn Gly Glu Arg Arg Tyr Ile 
    50                  55                  60                  


Thr Leu Trp Lys Asn Thr Thr Pro Lys Asp Val Phe Thr Tyr Asp Tyr 
65                  70                  75                  80  


Ala Thr Gly Ser Thr Tyr Ile Phe Thr Asn Ile Asp Tyr Glu Val Lys 
                85                  90                  95      


Asp Gly Tyr Glu Asn Leu Thr Ala Thr Tyr Gln Thr Thr Val Glu Asn 
            100                 105                 110         


Ala Thr Ala Gln Glu Val Gly Thr Thr Asp Glu Asp Glu Thr Phe Ala 
        115                 120                 125             


Gly Gly Glu Pro Leu Asp His His Leu Asp Asp Ala Leu Asn Glu Thr 
    130                 135                 140                 


Pro Asp Asp Ala Glu Thr Glu Ser Asp Ser Gly His Val Met Thr Ser 
145                 150                 155                 160 


Phe Ala Ser Arg Asp Gln Leu Pro Glu Trp Thr Leu His Thr Tyr Thr 
                165                 170                 175     


Leu Thr Ala Thr Asp Gly Ala Lys Thr Asp Thr Glu Tyr Ala Arg Arg 
            180                 185                 190         


Thr Leu Ala Tyr Thr Val Arg Gln Glu Leu Tyr Thr Asp His Asp Ala 
        195                 200                 205             


Ala Pro Val Ala Thr Asp Gly Leu Met Leu Leu Thr Pro Glu Pro Leu 
    210                 215                 220                 


Gly Glu Thr Pro Leu Asp Leu Asp Cys Gly Val Arg Val Glu Ala Asp 
225                 230                 235                 240 


Glu Thr Arg Thr Leu Asp Tyr Thr Thr Ala Lys Asp Arg Leu Leu Ala 
                245                 250                 255     


Arg Glu Leu Val Glu Glu Gly Leu Lys Arg Ser Leu Trp Asp Asp Tyr 
            260                 265                 270         


Leu Val Arg Gly Ile Asp Glu Val Leu Ser Lys Glu Pro Val Leu Thr 
        275                 280                 285             


Cys Asp Glu Phe Asp Leu His Glu Arg Tyr Asp Leu Ser Val Glu Val 
    290                 295                 300                 


Gly His Ser Gly Arg Ala Tyr Leu His Ile Asn Phe Arg His Arg Phe 
305                 310                 315                 320 


Val Pro Lys Leu Thr Leu Ala Asp Ile Asp Asp Asp Asn Ile Tyr Pro 
                325                 330                 335     


Gly Leu Arg Val Lys Thr Thr Tyr Arg Pro Arg Arg Gly His Ile Val 
            340                 345                 350         


Trp Gly Leu Arg Asp Glu Cys Ala Thr Asp Ser Leu Asn Thr Leu Gly 
        355                 360                 365             


Asn Gln Ser Val Val Ala Tyr His Arg Asn Asn Gln Thr Pro Ile Asn 
    370                 375                 380                 


Thr Asp Leu Leu Asp Ala Ile Glu Ala Ala Asp Arg Arg Val Val Glu 
385                 390                 395                 400 


Thr Arg Arg Gln Gly His Gly Asp Asp Ala Val Ser Phe Pro Gln Glu 
                405                 410                 415     


Leu Leu Ala Val Glu Pro Asn Thr His Gln Ile Lys Gln Phe Ala Ser 
            420                 425                 430         


Asp Gly Phe His Gln Gln Ala Arg Ser Lys Thr Arg Leu Ser Ala Ser 
        435                 440                 445             


Arg Cys Ser Glu Lys Ala Gln Ala Phe Ala Glu Arg Leu Asp Pro Val 
    450                 455                 460                 


Arg Leu Asn Gly Ser Thr Val Glu Phe Ser Ser Glu Phe Phe Thr Gly 
465                 470                 475                 480 


Asn Asn Glu Gln Gln Leu Arg Leu Leu Tyr Glu Asn Gly Glu Ser Val 
                485                 490                 495     


Leu Thr Phe Arg Asp Gly Ala Arg Gly Ala His Pro Asp Glu Thr Phe 
            500                 505                 510         


Ser Lys Gly Ile Val Asn Pro Pro Glu Ser Phe Glu Val Ala Val Val 
        515                 520                 525             


Leu Pro Glu Gln Gln Ala Asp Thr Cys Lys Ala Gln Trp Asp Thr Met 
    530                 535                 540                 


Ala Asp Leu Leu Asn Gln Ala Gly Ala Pro Pro Thr Arg Ser Glu Thr 
545                 550                 555                 560 


Val Gln Tyr Asp Ala Phe Ser Ser Pro Glu Ser Ile Ser Leu Asn Val 
                565                 570                 575     


Ala Gly Ala Ile Asp Pro Ser Glu Val Asp Ala Ala Phe Val Val Leu 
            580                 585                 590         


Pro Pro Asp Gln Glu Gly Phe Ala Asp Leu Ala Ser Pro Thr Glu Thr 
        595                 600                 605             


Tyr Asp Glu Leu Lys Lys Ala Leu Ala Asn Met Gly Ile Tyr Ser Gln 
    610                 615                 620                 


Met Ala Tyr Phe Asp Arg Phe Arg Asp Ala Lys Ile Phe Tyr Thr Arg 
625                 630                 635                 640 


Asn Val Ala Leu Gly Leu Leu Ala Ala Ala Gly Gly Val Ala Phe Thr 
                645                 650                 655     


Thr Glu His Ala Met Pro Gly Asp Ala Asp Met Phe Ile Gly Ile Asp 
            660                 665                 670         


Val Ser Arg Ser Tyr Pro Glu Asp Gly Ala Ser Gly Gln Ile Asn Ile 
        675                 680                 685             


Ala Ala Thr Ala Thr Ala Val Tyr Lys Asp Gly Thr Ile Leu Gly His 
    690                 695                 700                 


Ser Ser Thr Arg Pro Gln Leu Gly Glu Lys Leu Gln Ser Thr Asp Val 
705                 710                 715                 720 


Arg Asp Ile Met Lys Asn Ala Ile Leu Gly Tyr Gln Gln Val Thr Gly 
                725                 730                 735     


Glu Ser Pro Thr His Ile Val Ile His Arg Asp Gly Phe Met Asn Glu 
            740                 745                 750         


Asp Leu Asp Pro Ala Thr Glu Phe Leu Asn Glu Gln Gly Val Glu Tyr 
        755                 760                 765             


Asp Ile Val Glu Ile Arg Lys Gln Pro Gln Thr Arg Leu Leu Ala Val 
    770                 775                 780                 


Ser Asp Val Gln Tyr Asp Thr Pro Val Lys Ser Ile Ala Ala Ile Asn 
785                 790                 795                 800 


Gln Asn Glu Pro Arg Ala Thr Val Ala Thr Phe Gly Ala Pro Glu Tyr 
                805                 810                 815     


Leu Ala Thr Arg Asp Gly Gly Gly Leu Pro Arg Pro Ile Gln Ile Glu 
            820                 825                 830         


Arg Val Ala Gly Glu Thr Asp Ile Glu Thr Leu Thr Arg Gln Val Tyr 
        835                 840                 845             


Leu Leu Ser Gln Ser His Ile Gln Val His Asn Ser Thr Ala Arg Leu 
    850                 855                 860                 


Pro Ile Thr Thr Ala Tyr Ala Asp Gln Ala Ser Thr His Ala Thr Lys 
865                 870                 875                 880 


Gly Tyr Leu Val Gln Thr Gly Ala Phe Glu Ser Asn Val Gly Phe Leu 
                885                 890                 895     


<210>  5
<211>  736
<212>  PRT
<213>  Clostridium bartletti

<400>  5

Met Val Ser Leu Asp Arg Glu Phe Asn Val Ile Thr Glu Phe Lys Asn 
1               5                   10                  15      


Glu Leu Lys Pro Glu Asp Ile Lys Ile Phe Leu Tyr Ser Met Pro Ile 
            20                  25                  30          


Lys Asp Ile Asn Glu Arg His Ser Glu Asn Tyr Ala Ile Val Gln Glu 
        35                  40                  45              


Leu Lys Lys Ile Asn Glu Asn Pro Asn Ile Val Phe Asn Glu Tyr Ile 
    50                  55                  60                  


Ile Ala Ser Phe Asn Pro Ile Ile Asn Trp Gly Lys Tyr Lys Asp Ile 
65                  70                  75                  80  


Asp Val Lys Pro Asp Asn Arg Asn Ile Asn Leu Asp Asn His Thr Glu 
                85                  90                  95      


Arg Lys Ile Leu Glu Arg Leu Leu Leu Cys Asp Ile Lys Asn Asn Ile 
            100                 105                 110         


Asn Asn Asn Thr Thr Trp Glu Gln Gln Asn Lys Tyr Glu Ile Arg Gly 
        115                 120                 125             


Asn Ala Asn Pro Ala Val Tyr Leu Arg Arg Pro Ile Tyr Ser Asn Asn 
    130                 135                 140                 


Asn Leu Ile Ile Arg Arg Lys Leu Asn Phe Asp Val Asn Ile Asp Lys 
145                 150                 155                 160 


Lys Asp Ile Ile Ile Gly Phe Phe Leu Asn His Glu Phe Glu Tyr Gln 
                165                 170                 175     


Lys Thr Leu Asp Glu Glu Ile Lys Cys Gly Asn Ile Gln Lys Gly Asp 
            180                 185                 190         


Lys Val Lys Asp Phe Tyr Asn Asn Ile Thr Tyr Glu Phe Leu Glu Ile 
        195                 200                 205             


Ala Pro Phe Ser Ile Ser Gln Glu Asn Lys Tyr Met Arg Ser Ser Ile 
    210                 215                 220                 


Ile Glu Tyr Tyr Leu Asn Lys Gly Gln Ser Tyr Ile Ile Ser Gly Leu 
225                 230                 235                 240 


Asp Lys Asn Thr Lys Ala Val Leu Val Lys Asn Lys Glu Gly Ser Ile 
                245                 250                 255     


Phe Pro Tyr Ile Pro Asn Arg Leu Lys Lys Ile Cys Val Phe Glu Asn 
            260                 265                 270         


Leu Gly Asn Arg Arg Ile Ile Glu Gly Asn Lys Tyr Ile Lys Met Asn 
        275                 280                 285             


Pro Ser Gln Asn Met Ser Glu Ser Ile Lys Leu Ala Glu Gly Ile Leu 
    290                 295                 300                 


Lys Asn Ser Lys Tyr Val Lys Phe Asn Lys Ala Asn Met Ile Val Glu 
305                 310                 315                 320 


Lys Ile Gly Tyr Lys Lys Asp Ile Val Lys Arg Pro Ala Leu Lys Phe 
                325                 330                 335     


Gly Lys Asn Glu Ser Asn Phe Ser Ala Met Tyr Gly Leu Asn Lys Ser 
            340                 345                 350         


Gly Ser Tyr Glu Gln Lys Asn Ile Lys Ile Asp Tyr Phe Ile Asp Pro 
        355                 360                 365             


Lys Ile Leu Asn Asn Lys Arg Asp Tyr Gln Ile Val Tyr Ser Phe Leu 
    370                 375                 380                 


Asn Asp Ile Ile Ser Lys Ser Lys Asp Leu Gly Val Glu Ile Asn Thr 
385                 390                 395                 400 


Asp Lys Ser Tyr Ile Asn Leu Thr Pro Ile Asn Ile Lys Asn Glu Asn 
                405                 410                 415     


Val Phe Glu Leu Asn Ile Ile Gln Ile Ile Glu Asn Tyr Asn Asn Pro 
            420                 425                 430         


Val Leu Val Ile Leu Glu Lys Glu Asn Ile Asp Lys Tyr Tyr Glu Thr 
        435                 440                 445             


Leu Lys Lys Ile Phe Gly Gly Arg Asn Asn Ile Pro Thr Gln Phe Val 
    450                 455                 460                 


Asp Leu Asp Thr Ile Lys Lys Cys Asp Pro Lys Ile Asp Asn Lys Arg 
465                 470                 475                 480 


Gly Lys Glu Ser Ile Phe Leu Asn Ile Leu Leu Gly Ile Tyr Cys Lys 
                485                 490                 495     


Ser Gly Ile Gln Pro Trp Val Leu Ala Asn Gly Leu Ser Ala Asp Cys 
            500                 505                 510         


Tyr Ile Gly Leu Asp Val Cys Arg Glu Asn Asn Met Ser Thr Ala Gly 
        515                 520                 525             


Leu Ile Gln Val Ile Gly Lys Asp Gly Arg Val Leu Lys Ser Lys Thr 
    530                 535                 540                 


Ile Ser Ser His Gln Ser Gly Glu Lys Ile Gln Ile Asn Ile Leu Lys 
545                 550                 555                 560 


Asp Ile Ile Phe Glu Ala Lys Gln Ala Tyr Lys Asn Thr Tyr Asn Lys 
                565                 570                 575     


Lys Leu Glu His Ile Val Phe His Arg Asp Gly Ile Asn Arg Glu Asp 
            580                 585                 590         


Ile Asp Leu Leu Lys Glu Ile Thr Asn Ser Leu Glu Ile Lys Phe Asp 
        595                 600                 605             


Tyr Val Glu Val Thr Lys Asn Ile Asn Arg Arg Met Ala Met Leu Glu 
    610                 615                 620                 


Lys Ser Asp Glu Asn Tyr Asn His Arg Asp Lys Glu Asn Lys Lys Trp 
625                 630                 635                 640 


Ile Thr Glu Ile Gly Met Cys Leu Lys Lys Glu Asn Glu Ala Tyr Leu 
                645                 650                 655     


Ile Thr Thr Asn Pro Ser Glu Asn Met Gly Met Ala Arg Pro Leu Arg 
            660                 665                 670         


Ile Lys Lys Val Tyr Gly Asn Gln Asn Met Asp Asp Ile Val Lys Asp 
        675                 680                 685             


Ile Tyr Lys Leu Ser Phe Met His Ile Gly Ser Ile Met Lys Ser Arg 
    690                 695                 700                 


Leu Pro Ile Thr Thr His Tyr Ala Asp Leu Ser Ser Ile Tyr Ser His 
705                 710                 715                 720 


Arg Glu Leu Met Pro Lys Ser Val Asp Asn Asn Ile Leu His Phe Ile 
                725                 730                 735     


<210>  6
<211>  735
<212>  PRT
<213>  Synechococcus elongatus

<400>  6

Met Asp Leu Leu Ser Asn Leu Arg Arg Ser Ser Ile Val Leu Asn Arg 
1               5                   10                  15      


Phe Tyr Val Lys Ser Leu Ser Gln Ser Asp Leu Thr Ala Tyr Glu Tyr 
            20                  25                  30          


Arg Cys Ile Phe Lys Lys Thr Pro Glu Leu Gly Asp Glu Lys Arg Leu 
        35                  40                  45              


Leu Ala Ser Ile Cys Tyr Lys Leu Gly Ala Ile Ala Val Arg Ile Gly 
    50                  55                  60                  


Ser Asn Ile Ile Thr Lys Glu Ala Val Arg Pro Glu Lys Leu Gln Gly 
65                  70                  75                  80  


His Asp Trp Gln Leu Val Gln Met Gly Thr Lys Gln Leu Asp Cys Arg 
                85                  90                  95      


Asn Asp Ala His Arg Cys Ala Leu Glu Thr Phe Glu Arg Lys Phe Leu 
            100                 105                 110         


Glu Arg Asp Leu Ser Ala Ser Ser Gln Thr Glu Val Arg Lys Ala Ala 
        115                 120                 125             


Glu Gly Gly Leu Ile Trp Trp Val Val Gly Ala Lys Gly Ile Glu Lys 
    130                 135                 140                 


Ser Gly Asn Gly Trp Glu Val His Arg Gly Arg Arg Ile Asp Val Ser 
145                 150                 155                 160 


Leu Asp Ala Glu Gly Asn Leu Tyr Leu Glu Ile Asp Ile His His Arg 
                165                 170                 175     


Phe Tyr Thr Pro Trp Thr Val His Gln Trp Leu Glu Gln Tyr Pro Glu 
            180                 185                 190         


Ile Pro Leu Ser Tyr Val Arg Asn Asn Tyr Leu Asp Glu Arg His Gly 
        195                 200                 205             


Phe Ile Asn Trp Gln Tyr Gly Arg Phe Thr Gln Glu Arg Pro Gln Asp 
    210                 215                 220                 


Ile Leu Leu Asp Cys Leu Gly Met Ser Leu Ala Glu Tyr His Leu Asn 
225                 230                 235                 240 


Lys Gly Ala Thr Glu Glu Glu Val Gln Gln Ser Tyr Val Val Tyr Val 
                245                 250                 255     


Lys Pro Ile Ser Trp Arg Lys Gly Lys Leu Thr Ala His Leu Ser Arg 
            260                 265                 270         


Arg Leu Ser Pro Ser Leu Thr Met Glu Met Leu Ala Lys Val Ala Glu 
        275                 280                 285             


Asp Ser Thr Val Cys Asp Arg Glu Lys Arg Glu Ile Arg Ala Val Phe 
    290                 295                 300                 


Lys Ser Ile Lys Gln Ser Ile Asn Gln Arg Leu Gln Glu Ala Gln Lys 
305                 310                 315                 320 


Thr Ala Ser Trp Ile Leu Thr Lys Thr Tyr Gly Ile Ser Ser Pro Ala 
                325                 330                 335     


Ile Ala Leu Ser Cys Asp Gly Tyr Leu Leu Pro Ala Ala Lys Leu Leu 
            340                 345                 350         


Ala Ala Asn Lys Gln Pro Val Ser Lys Thr Ala Asp Ile Arg Asn Lys 
        355                 360                 365             


Gly Cys Ala Lys Ile Gly Glu Thr Ser Phe Gly Tyr Leu Asn Leu Tyr 
    370                 375                 380                 


Asn Asn Gln Leu Gln Tyr Pro Leu Glu Val His Lys Cys Leu Leu Glu 
385                 390                 395                 400 


Ile Ala Asn Lys Asn Asn Leu Gln Leu Ser Leu Asp Gln Arg Arg Val 
                405                 410                 415     


Leu Ser Asp Tyr Pro Gln Asp Asp Leu Asp Gln Gln Met Phe Trp Gln 
            420                 425                 430         


Thr Trp Ser Ser Gln Gly Ile Lys Thr Val Leu Val Val Met Pro Trp 
        435                 440                 445             


Asp Ser His His Asp Lys Gln Lys Ile Arg Ile Gln Ala Ile Gln Ala 
    450                 455                 460                 


Gly Ile Ala Thr Gln Phe Met Val Pro Leu Pro Lys Ala Asp Lys Tyr 
465                 470                 475                 480 


Lys Ala Leu Asn Val Thr Leu Gly Leu Leu Cys Lys Ala Gly Trp Gln 
                485                 490                 495     


Pro Ile Gln Leu Glu Ser Val Asp His Pro Glu Val Ala Asp Leu Ile 
            500                 505                 510         


Ile Gly Phe Asp Thr Gly Thr Asn Arg Glu Leu Tyr Tyr Gly Thr Ser 
        515                 520                 525             


Ala Phe Ala Val Leu Ala Asp Gly Gln Ser Leu Gly Trp Glu Leu Pro 
    530                 535                 540                 


Ala Val Gln Gly Gly Glu Thr Phe Ser Gly Gln Ala Ile Trp Gln Thr 
545                 550                 555                 560 


Val Ser Lys Leu Ile Ile Lys Phe Tyr Gln Ile Cys Gln Arg Tyr Pro 
                565                 570                 575     


Gln Lys Leu Leu Leu Met Arg Asp Gly Leu Val Gln Glu Gly Glu Phe 
            580                 585                 590         


Gln Gln Thr Ile Glu Leu Leu Lys Glu Arg Lys Ile Ala Val Asp Val 
        595                 600                 605             


Ile Ser Val Arg Lys Ser Gly Ala Gly Arg Met Gly Gln Glu Ile Tyr 
    610                 615                 620                 


Glu Asn Gly Gln Leu Val Tyr Arg Asp Ala Ala Ile Gly Ser Val Ile 
625                 630                 635                 640 


Leu Gln Pro Ala Glu Arg Ser Phe Ile Met Val Thr Ser Gln Pro Val 
                645                 650                 655     


Ser Lys Thr Ile Gly Ser Ile Arg Pro Leu Arg Ile Val His Glu Tyr 
            660                 665                 670         


Gly Ser Thr Asp Leu Glu Leu Leu Ala Leu Gln Thr Tyr His Leu Thr 
        675                 680                 685             


Gln Leu His Pro Ala Ser Gly Phe Arg Ser Cys Arg Leu Pro Trp Val 
    690                 695                 700                 


Leu His Leu Ala Asp Arg Ser Ser Lys Glu Phe Gln Arg Ile Gly Gln 
705                 710                 715                 720 


Ile Ser Val Leu Gln Asn Ile Ser Arg Asp Lys Leu Ile Ala Val 
                725                 730                 735 


<210>  7
<211>  3961
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  DNA Sequence of pWUR704

<400>  7
gtcgacttta tatttaaata atttaatata ctatacaacc tactacctcg tataaatttt       60

taaataaata ttgcattcaa gcttttaatt taattaaatg gccgctctag aggcatcaaa      120

taaaacgaaa ggctcagtcg aaagactggg cctttcgttt tatctgttgt ttgtcggtga      180

acgctctcct gagtaggaca aatccgccgc cctagaccta gggtacgggt tttgctgccc      240

gcaaacgggc tgttctggtg ttgctagttt gttatcagaa tcgcagatcc ggcttcaggt      300

ttgccggctg aaagcgctat ttcttccaga attgccatga ttttttcccc acgggaggcg      360

tcactggctc ccgtgttgtc ggcagctttg attcgataag cagcatcgcc tgtttcaggc      420

tgtctatgtg tgactgttga gctgtaacaa gttgtctcag gtgttcaatt tcatgttcta      480

gttgctttgt tttactggtt tcacctgttc tattaggtgt tacatgctgt tcatctgtta      540

cattgtcgat ctgttcatgg tgaacagctt taaatgcacc aaaaactcgt aaaagctctg      600

atgtatctat cttttttaca ccgttttcat ctgtgcatat ggacagtttt ccctttgata      660

tctaacggtg aacagttgtt ctacttttgt ttgttagtct tgatgcttca ctgatagata      720

caagagccat aagaacctca gatccttccg tatttagcca gtatgttctc tagtgtggtt      780

cgttgttttt gcgtgagcca tgagaacgaa ccattgagat catgcttact ttgcatgtca      840

ctcaaaaatt ttgcctcaaa actggtgagc tgaatttttg cagttaaagc atcgtgtagt      900

gtttttctta gtccgttacg taggtaggaa tctgatgtaa tggttgttgg tattttgtca      960

ccattcattt ttatctggtt gttctcaagt tcggttacga gatccatttg tctatctagt     1020

tcaacttgga aaatcaacgt atcagtcggg cggcctcgct tatcaaccac caatttcata     1080

ttgctgtaag tgtttaaatc tttacttatt ggtttcaaaa cccattggtt aagcctttta     1140

aactcatggt agttattttc aagcattaac atgaacttaa attcatcaag gctaatctct     1200

atatttgcct tgtgagtttt cttttgtgtt agttctttta ataaccactc ataaatcctc     1260

atagagtatt tgttttcaaa agacttaaca tgttccagat tatattttat gaattttttt     1320

aactggaaaa gataaggcaa tatctcttca ctaaaaacta attctaattt ttcgcttgag     1380

aacttggcat agtttgtcca ctggaaaatc tcaaagcctt taaccaaagg attcctgatt     1440

tccacagttc tcgtcatcag ctctctggtt gctttagcta atacaccata agcattttcc     1500

ctactgatgt tcatcatctg agcgtattgg ttataagtga acgataccgt ccgttctttc     1560

cttgtagggt tttcaatcgt ggggttgagt agtgccacac agcataaaat tagcttggtt     1620

tcatgctccg ttaagtcata gcgactaatc gctagttcat ttgctttgaa aacaactaat     1680

tcagacatac atctcaattg gtctaggtga ttttaatcac tataccaatt gagatgggct     1740

agtcaatgat aattactagt ccttttcccg ggagatctgg gtatctgtaa attctgctag     1800

acctttgctg gaaaacttgt aaattctgct agaccctctg taaattccgc tagacctttg     1860

tgtgtttttt ttgtttatat tcaagtggtt ataatttata gaataaagaa agaataaaaa     1920

aagataaaaa gaatagatcc cagccctgtg tataactcac tactttagtc agttccgcag     1980

tattacaaaa ggatgtcgca aacgctgttt gctcctctac aaaacagacc ttaaaaccct     2040

aaaggcttaa gtagcaccct cgcaagctcg ggcaaatcgc tgaatattcc ttttgtctcc     2100

gaccatcagg cacctgagtc gctgtctttt tcgtgacatt cagttcgctg cgctcacggc     2160

tctggcagtg aatgggggta aatggcacta caggcgcctt ttatggattc atgcaaggaa     2220

actacccata atacaagaaa agcccgtcac gggcttctca gggcgtttta tggcgggtct     2280

gctatgtggt gctatctgac tttttgctgt tcagcagttc ctgccctctg attttccagt     2340

ctgaccactt cggattatcc cgtgacaggt cattcagact ggctaatgca cccagtaagg     2400

cagcggtatc atcaacaggc ttacccgtct tactgtccct agtgcttgga ttctcaccaa     2460

taaaaaacgc ccggcggcaa ccgagcgttc tgaacaaatc cagatggagt tctgaggtca     2520

ttactggatc tatcaacagg agtccaagcg agctctccgt gtcgttctgt ccactcctga     2580

atcccattcc agaaattctc tagcgattcc agaagtttct cagagtcgga aagttgacca     2640

gacattacga actggcacag atggtcataa cctgaaggaa gatctctatt cctttgccct     2700

cggacgagtg ctggggcgtc ggtttccact atcggcgagt acttctacac agccatcggt     2760

ccagacggcc gcgcttctgc gggcgatttg tgtacgcccg acagtcccgg ctccggatcg     2820

gacgattgcg tcgcatcgac cctgcgccca agctgcatca tcgaaattgc cgtcaaccaa     2880

gctctgatag agttggtcaa gaccaatgcg gagcatatac gcccggagcc gcggcgatcc     2940

tgcaagctcc ggatgcctcc gctcgaagta gcgcgcctgc tgctccatac aagccaacca     3000

cggcctccag aagaagatgt tggcgacctc gtatagggga tctccgaaca tcgcctcgct     3060

ccagtcaatg accgctgtta tgcggccatt gtccgtcagg acattgttgg agccgaaatc     3120

cgcgtgcacg aggtgccgga cttcggggca gtcctcggcc caaagcatca gctcatcgag     3180

agcctgcgcg acggacgcac tgacggtgtc gtccatcaca gtttgccagt gatacacatg     3240

gggatcagca atcgcgcaga tgaaatcacg ccatgtagtg tattgaccga ttccttgcgg     3300

tccgaatggg ccgaacccgc tcgtctggct aagatcggcc gcagcgatcg catccataac     3360

ctccgcgacc ggttgcagaa cagcgggcag ttcggtttca ggcaggtctt gcaacgtgac     3420

accctgtgca cggcgggaga tgcaataggt caggctctcg ctaaattccc caatgtcaag     3480

cacttccgga atcgggagcg cggccgatgc aaagtgccga taaacataac gatctttgta     3540

gaaaccatcg gcgcagctat ttacccgcag gacatatcca cgccctccta catcgaagct     3600

gaaagcacga gattcttcgc cctccgagag ctgcatcagg tcggagacgc tgccgaactt     3660

ttcgatcaga aacttctcaa cagacgtcgc ggtgagttca ggctttttca tgtgcctcac     3720

acctccttaa gggtcgtggg cgggaacccg agacgggcga gttgccgcgt ttcctctccg     3780

cccaggtccg cccggtgcgg ggaaaacccc ccaaaaggag ccctttttcc ccgcatccgg     3840

cgctatcgta aaaacctcac gcgcccttgt caaacggtcg ggccttaagg tttctgttat     3900

actccccccg gggatcgatc cccggcccga cgggagccgg gcggtggtgg cctgggctag     3960

c                                                                     3961


<210>  8
<211>  2281
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  DNA sequence of codon optimised CbAgo with LIC flanks

<400>  8
tacttccaat ccaatgcaaa taatctgacc tttgaggctt ttgaagggat tggtcaactg       60

aatgaactga atttttataa gtatcgtctg attgggaaag ggcagattga taatgtgcat      120

caagctattt ggtctgttaa atataaactc caggctaata atttttttaa accggtgttt      180

gtgaaaggtg agattctata tagcctagat gaactgaagg tgattccgga atttgaaaat      240

gttgaggtga ttctcgatgg taatattatt ctgtctattt ctgagaatac cgatatttat      300

aaggatgtga ttgtgttcta tattaacaat gctctcaaaa atattaaaga tattactaat      360

tatcgtaagt acattaccaa aaataccgat gagattatat gtaaatctat tctgactacc      420

aatctgaaat atcagtatat gaaatctgaa aaagggttta aactgcagag gaaatttaaa      480

attagcccgg ttgtttttcg taatgggaag gtgattctgt atctcaattg tagcagcgat      540

ttttctaccg ataagtctat atatgagatg ctaaatgatg gtctgggggt ggtgggtcta      600

caagtgaaaa ataaatggac caatgcaaat gggaatattt tcattgaaaa agtactggat      660

aataccattt ctgacccggg tacttctggt aagctaggtc agtctctaat tgattactat      720

attaatggta atcaaaagta tcgtgttgaa aagtttaccg atgaagataa aaatgcaaag      780

gtgattcagg ctaagattaa gaataaaacc tataattata ttccgcaagc tctgaccccg      840

gtgattacca gggaatatct gagccatacc gataagaagt tttctaagca gattgagaat      900

gtgattaaaa tggacatgaa ttatcgttat cagaccctaa aatctttcgt tgaagatatt      960

ggtgtgatta aagaactcaa taatctgcat tttaaaaatc agtattatac taattttgat     1020

tttatggggt ttgaatctgg tgttctggaa gaaccggtac tgatgggggc taacggtaaa     1080

attaaagata agaagcaaat ttttattaat gggtttttca agaatccgaa ggagaatgtg     1140

aagtttggtg ttctgtatcc ggaagggtgt atggaaaatg ctcagtcgat tgctcgttct     1200

atactagatt ttgcaaccgc tgggaaatat aataaacaag agaataaata tattagcaag     1260

aatctaatga atattggttt taagccgtct gaatgtattt tcgaatctta taaactcggt     1320

gatattaccg aatataaagc aaccgctcgt aagctaaaag aacatgaaaa ggtgggtttt     1380

gtgattgcag tgattccgga tatgaatgaa ctggaagtgg aaaatccgta taatccgttt     1440

aaaaaagtat gggcaaaact gaatattccg agccagatga ttaccctgaa aaccaccgaa     1500

aaatttaaaa atattgttga taagtctggt ctatattatc tacacaatat tgctctcaat     1560

attctaggga aaattggggg gattccgtgg attattaaag atatgccggg taatattgat     1620

tgtttcattg ggctagatgt gggtacccgt gaaaaaggta ttcattttcc ggcatgtagc     1680

gttctatttg ataaatatgg gaaactgatt aattattata agccgaccat tccgcagtct     1740

ggtgagaaaa ttgcagaaac cattctgcaa gagattttcg ataatgttct gattagctat     1800

aaagaagaaa atggggaata tccgaaaaat attgtgattc atcgtgatgg gttttctcgt     1860

gaaaatattg attggtataa agaatatttt gataagaaag ggattaaatt taacattatt     1920

gaggtgaaaa agaatattcc ggttaaaatt gcaaaggtgg ttgggtcgaa catttgtaat     1980

cccattaaag gttcttatgt tctcaaaaat gataaggctt ttattgttac caccgatatt     2040

aaagatgggg ttgctagccc gaaccccctg aaaattgaaa agacctatgg tgacgtggag     2100

atgaaaagca ttctagaaca gatttatagc ctgtctcaaa ttcatgttgg gagcaccaag     2160

tctctaaggc tcccgattac caccggttat gctgataaaa tttgcaaagc aattgagtac     2220

attccgcaag gtgttgttga taatcgtcta ttctttctgt aataacattg gaagtggata     2280

a                                                                     2281


<210>  9
<211>  2247
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  DNA sequence of codon optimised CbAgo

<400>  9
atgaataatc tgacctttga ggcttttgaa gggattggtc aactgaatga actgaatttt       60

tataagtatc gtctgattgg gaaagggcag attgataatg tgcatcaagc tatttggtct      120

gttaaatata aactccaggc taataatttt tttaaaccgg tgtttgtgaa aggtgagatt      180

ctatatagcc tagatgaact gaaggtgatt ccggaatttg aaaatgttga ggtgattctc      240

gatggtaata ttattctgtc tatttctgag aataccgata tttataagga tgtgattgtg      300

ttctatatta acaatgctct caaaaatatt aaagatatta ctaattatcg taagtacatt      360

accaaaaata ccgatgagat tatatgtaaa tctattctga ctaccaatct gaaatatcag      420

tatatgaaat ctgaaaaagg gtttaaactg cagaggaaat ttaaaattag cccggttgtt      480

tttcgtaatg ggaaggtgat tctgtatctc aattgtagca gcgatttttc taccgataag      540

tctatatatg agatgctaaa tgatggtctg ggggtggtgg gtctacaagt gaaaaataaa      600

tggaccaatg caaatgggaa tattttcatt gaaaaagtac tggataatac catttctgac      660

ccgggtactt ctggtaagct aggtcagtct ctaattgatt actatattaa tggtaatcaa      720

aagtatcgtg ttgaaaagtt taccgatgaa gataaaaatg caaaggtgat tcaggctaag      780

attaagaata aaacctataa ttatattccg caagctctga ccccggtgat taccagggaa      840

tatctgagcc ataccgataa gaagttttct aagcagattg agaatgtgat taaaatggac      900

atgaattatc gttatcagac cctaaaatct ttcgttgaag atattggtgt gattaaagaa      960

ctcaataatc tgcattttaa aaatcagtat tatactaatt ttgattttat ggggtttgaa     1020

tctggtgttc tggaagaacc ggtactgatg ggggctaacg gtaaaattaa agataagaag     1080

caaattttta ttaatgggtt tttcaagaat ccgaaggaga atgtgaagtt tggtgttctg     1140

tatccggaag ggtgtatgga aaatgctcag tcgattgctc gttctatact agattttgca     1200

accgctggga aatataataa acaagagaat aaatatatta gcaagaatct aatgaatatt     1260

ggttttaagc cgtctgaatg tattttcgaa tcttataaac tcggtgatat taccgaatat     1320

aaagcaaccg ctcgtaagct aaaagaacat gaaaaggtgg gttttgtgat tgcagtgatt     1380

ccggatatga atgaactgga agtggaaaat ccgtataatc cgtttaaaaa agtatgggca     1440

aaactgaata ttccgagcca gatgattacc ctgaaaacca ccgaaaaatt taaaaatatt     1500

gttgataagt ctggtctata ttatctacac aatattgctc tcaatattct agggaaaatt     1560

ggggggattc cgtggattat taaagatatg ccgggtaata ttgattgttt cattgggcta     1620

gatgtgggta cccgtgaaaa aggtattcat tttccggcat gtagcgttct atttgataaa     1680

tatgggaaac tgattaatta ttataagccg accattccgc agtctggtga gaaaattgca     1740

gaaaccattc tgcaagagat tttcgataat gttctgatta gctataaaga agaaaatggg     1800

gaatatccga aaaatattgt gattcatcgt gatgggtttt ctcgtgaaaa tattgattgg     1860

tataaagaat attttgataa gaaagggatt aaatttaaca ttattgaggt gaaaaagaat     1920

attccggtta aaattgcaaa ggtggttggg tcgaacattt gtaatcccat taaaggttct     1980

tatgttctca aaaatgataa ggcttttatt gttaccaccg atattaaaga tggggttgct     2040

agcccgaacc ccctgaaaat tgaaaagacc tatggtgacg tggagatgaa aagcattcta     2100

gaacagattt atagcctgtc tcaaattcat gttgggagca ccaagtctct aaggctcccg     2160

attaccaccg gttatgctga taaaatttgc aaagcaattg agtacattcc gcaaggtgtt     2220

gttgataatc gtctattctt tctgtaa                                         2247


