                         SEQUENCE LISTING

<110>  GlaxoSmithKline Intellectual Property Development Limited
 
<120>  NOVEL PROCESSES FOR THE PRODUCTION OF OLIGONUCLEOTIDES

<130>  PB66127

<160>  62    

<170>  PatentIn version 3.5

<210>  1
<211>  20
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Example 1 desired product oligonucleotide sequence ("target")

<400>  1
ggccaaacct cggcttacct                                                   20


<210>  2
<211>  23
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Example 1-4, 6, 10 and 11 template oligonucleotide sequence

<400>  2
tttaggtaag ccgaggtttg gcc                                               23


<210>  3
<211>  487
<212>  PRT
<213>  Enterobacteria phage T4

<400>  3

Met Ile Leu Lys Ile Leu Asn Glu Ile Ala Ser Ile Gly Ser Thr Lys 
1               5                   10                  15      


Gln Lys Gln Ala Ile Leu Glu Lys Asn Lys Asp Asn Glu Leu Leu Lys 
            20                  25                  30          


Arg Val Tyr Arg Leu Thr Tyr Ser Arg Gly Leu Gln Tyr Tyr Ile Lys 
        35                  40                  45              


Lys Trp Pro Lys Pro Gly Ile Ala Thr Gln Ser Phe Gly Met Leu Thr 
    50                  55                  60                  


Leu Thr Asp Met Leu Asp Phe Ile Glu Phe Thr Leu Ala Thr Arg Lys 
65                  70                  75                  80  


Leu Thr Gly Asn Ala Ala Ile Glu Glu Leu Thr Gly Tyr Ile Thr Asp 
                85                  90                  95      


Gly Lys Lys Asp Asp Val Glu Val Leu Arg Arg Val Met Met Arg Asp 
            100                 105                 110         


Leu Glu Cys Gly Ala Ser Val Ser Ile Ala Asn Lys Val Trp Pro Gly 
        115                 120                 125             


Leu Ile Pro Glu Gln Pro Gln Met Leu Ala Ser Ser Tyr Asp Glu Lys 
    130                 135                 140                 


Gly Ile Asn Lys Asn Ile Lys Phe Pro Ala Phe Ala Gln Leu Lys Ala 
145                 150                 155                 160 


Asp Gly Ala Arg Cys Phe Ala Glu Val Arg Gly Asp Glu Leu Asp Asp 
                165                 170                 175     


Val Arg Leu Leu Ser Arg Ala Gly Asn Glu Tyr Leu Gly Leu Asp Leu 
            180                 185                 190         


Leu Lys Glu Glu Leu Ile Lys Met Thr Ala Glu Ala Arg Gln Ile His 
        195                 200                 205             


Pro Glu Gly Val Leu Ile Asp Gly Glu Leu Val Tyr His Glu Gln Val 
    210                 215                 220                 


Lys Lys Glu Pro Glu Gly Leu Asp Phe Leu Phe Asp Ala Tyr Pro Glu 
225                 230                 235                 240 


Asn Ser Lys Ala Lys Glu Phe Ala Glu Val Ala Glu Ser Arg Thr Ala 
                245                 250                 255     


Ser Asn Gly Ile Ala Asn Lys Ser Leu Lys Gly Thr Ile Ser Glu Lys 
            260                 265                 270         


Glu Ala Gln Cys Met Lys Phe Gln Val Trp Asp Tyr Val Pro Leu Val 
        275                 280                 285             


Glu Ile Tyr Ser Leu Pro Ala Phe Arg Leu Lys Tyr Asp Val Arg Phe 
    290                 295                 300                 


Ser Lys Leu Glu Gln Met Thr Ser Gly Tyr Asp Lys Val Ile Leu Ile 
305                 310                 315                 320 


Glu Asn Gln Val Val Asn Asn Leu Asp Glu Ala Lys Val Ile Tyr Lys 
                325                 330                 335     


Lys Tyr Ile Asp Gln Gly Leu Glu Gly Ile Ile Leu Lys Asn Ile Asp 
            340                 345                 350         


Gly Leu Trp Glu Asn Ala Arg Ser Lys Asn Leu Tyr Lys Phe Lys Glu 
        355                 360                 365             


Val Ile Asp Val Asp Leu Lys Ile Val Gly Ile Tyr Pro His Arg Lys 
    370                 375                 380                 


Asp Pro Thr Lys Ala Gly Gly Phe Ile Leu Glu Ser Glu Cys Gly Lys 
385                 390                 395                 400 


Ile Lys Val Asn Ala Gly Ser Gly Leu Lys Asp Lys Ala Gly Val Lys 
                405                 410                 415     


Ser His Glu Leu Asp Arg Thr Arg Ile Met Glu Asn Gln Asn Tyr Tyr 
            420                 425                 430         


Ile Gly Lys Ile Leu Glu Cys Glu Cys Asn Gly Trp Leu Lys Ser Asp 
        435                 440                 445             


Gly Arg Thr Asp Tyr Val Lys Leu Phe Leu Pro Ile Ala Ile Arg Leu 
    450                 455                 460                 


Arg Glu Asp Lys Thr Lys Ala Asn Thr Phe Glu Asp Val Phe Gly Asp 
465                 470                 475                 480 


Phe His Glu Val Thr Gly Leu 
                485         


<210>  4
<211>  488
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Wild type T4 DNA ligase protein sequence (when fused to CBD)

<400>  4

Gly Ser Ile Leu Lys Ile Leu Asn Glu Ile Ala Ser Ile Gly Ser Thr 
1               5                   10                  15      


Lys Gln Lys Gln Ala Ile Leu Glu Lys Asn Lys Asp Asn Glu Leu Leu 
            20                  25                  30          


Lys Arg Val Tyr Arg Leu Thr Tyr Ser Arg Gly Leu Gln Tyr Tyr Ile 
        35                  40                  45              


Lys Lys Trp Pro Lys Pro Gly Ile Ala Thr Gln Ser Phe Gly Met Leu 
    50                  55                  60                  


Thr Leu Thr Asp Met Leu Asp Phe Ile Glu Phe Thr Leu Ala Thr Arg 
65                  70                  75                  80  


Lys Leu Thr Gly Asn Ala Ala Ile Glu Glu Leu Thr Gly Tyr Ile Thr 
                85                  90                  95      


Asp Gly Lys Lys Asp Asp Val Glu Val Leu Arg Arg Val Met Met Arg 
            100                 105                 110         


Asp Leu Glu Cys Gly Ala Ser Val Ser Ile Ala Asn Lys Val Trp Pro 
        115                 120                 125             


Gly Leu Ile Pro Glu Gln Pro Gln Met Leu Ala Ser Ser Tyr Asp Glu 
    130                 135                 140                 


Lys Gly Ile Asn Lys Asn Ile Lys Phe Pro Ala Phe Ala Gln Leu Lys 
145                 150                 155                 160 


Ala Asp Gly Ala Arg Cys Phe Ala Glu Val Arg Gly Asp Glu Leu Asp 
                165                 170                 175     


Asp Val Arg Leu Leu Ser Arg Ala Gly Asn Glu Tyr Leu Gly Leu Asp 
            180                 185                 190         


Leu Leu Lys Glu Glu Leu Ile Lys Met Thr Ala Glu Ala Arg Gln Ile 
        195                 200                 205             


His Pro Glu Gly Val Leu Ile Asp Gly Glu Leu Val Tyr His Glu Gln 
    210                 215                 220                 


Val Lys Lys Glu Pro Glu Gly Leu Asp Phe Leu Phe Asp Ala Tyr Pro 
225                 230                 235                 240 


Glu Asn Ser Lys Ala Lys Glu Phe Ala Glu Val Ala Glu Ser Arg Thr 
                245                 250                 255     


Ala Ser Asn Gly Ile Ala Asn Lys Ser Leu Lys Gly Thr Ile Ser Glu 
            260                 265                 270         


Lys Glu Ala Gln Cys Met Lys Phe Gln Val Trp Asp Tyr Val Pro Leu 
        275                 280                 285             


Val Glu Ile Tyr Ser Leu Pro Ala Phe Arg Leu Lys Tyr Asp Val Arg 
    290                 295                 300                 


Phe Ser Lys Leu Glu Gln Met Thr Ser Gly Tyr Asp Lys Val Ile Leu 
305                 310                 315                 320 


Ile Glu Asn Gln Val Val Asn Asn Leu Asp Glu Ala Lys Val Ile Tyr 
                325                 330                 335     


Lys Lys Tyr Ile Asp Gln Gly Leu Glu Gly Ile Ile Leu Lys Asn Ile 
            340                 345                 350         


Asp Gly Leu Trp Glu Asn Ala Arg Ser Lys Asn Leu Tyr Lys Phe Lys 
        355                 360                 365             


Glu Val Ile Asp Val Asp Leu Lys Ile Val Gly Ile Tyr Pro His Arg 
    370                 375                 380                 


Lys Asp Pro Thr Lys Ala Gly Gly Phe Ile Leu Glu Ser Glu Cys Gly 
385                 390                 395                 400 


Lys Ile Lys Val Asn Ala Gly Ser Gly Leu Lys Asp Lys Ala Gly Val 
                405                 410                 415     


Lys Ser His Glu Leu Asp Arg Thr Arg Ile Met Glu Asn Gln Asn Tyr 
            420                 425                 430         


Tyr Ile Gly Lys Ile Leu Glu Cys Glu Cys Asn Gly Trp Leu Lys Ser 
        435                 440                 445             


Asp Gly Arg Thr Asp Tyr Val Lys Leu Phe Leu Pro Ile Ala Ile Arg 
    450                 455                 460                 


Leu Arg Glu Asp Lys Thr Lys Ala Asn Thr Phe Glu Asp Val Phe Gly 
465                 470                 475                 480 


Asp Phe His Glu Val Thr Gly Leu 
                485             


<210>  5
<211>  20
<212>  RNA
<213>  Artificial sequence

<220>
<223>  Example 2 target sequence

<400>  5
ggccaaaccu cggcuuaccu                                                   20


<210>  6
<211>  482
<212>  PRT
<213>  Enterobacteria phase CC31

<400>  6

Met Ile Leu Asp Ile Ile Asn Glu Ile Ala Ser Ile Gly Ser Thr Lys 
1               5                   10                  15      


Glu Lys Glu Ala Ile Ile Arg Arg His Lys Asp Asn Glu Leu Leu Lys 
            20                  25                  30          


Arg Val Phe Arg Met Thr Tyr Asp Gly Lys Leu Gln Tyr Tyr Ile Lys 
        35                  40                  45              


Lys Trp Asp Thr Arg Pro Lys Gly Asp Ile His Leu Thr Leu Glu Asp 
    50                  55                  60                  


Met Leu Tyr Leu Leu Glu Glu Lys Leu Ala Lys Arg Val Val Thr Gly 
65                  70                  75                  80  


Asn Ala Ala Lys Glu Lys Leu Glu Ile Ala Leu Ser Gln Thr Ser Asp 
                85                  90                  95      


Ala Asp Ala Glu Val Val Lys Lys Val Leu Leu Arg Asp Leu Arg Cys 
            100                 105                 110         


Gly Ala Ser Arg Ser Ile Ala Asn Lys Val Trp Lys Asn Leu Ile Pro 
        115                 120                 125             


Glu Gln Pro Gln Met Leu Ala Ser Ser Tyr Asp Glu Lys Gly Ile Glu 
    130                 135                 140                 


Lys Asn Ile Lys Phe Pro Ala Phe Ala Gln Leu Lys Ala Asp Gly Ala 
145                 150                 155                 160 


Arg Ala Phe Ala Glu Val Arg Gly Asp Glu Leu Asp Asp Val Lys Ile 
                165                 170                 175     


Leu Ser Arg Ala Gly Asn Glu Tyr Leu Gly Leu Asp Leu Leu Lys Gln 
            180                 185                 190         


Gln Leu Ile Glu Met Thr Lys Glu Ala Arg Glu Arg His Pro Gly Gly 
        195                 200                 205             


Val Met Ile Asp Gly Glu Leu Val Tyr His Ala Ser Thr Leu Pro Ala 
    210                 215                 220                 


Gly Pro Leu Asp Asp Ile Phe Gly Asp Leu Pro Glu Leu Ser Lys Ala 
225                 230                 235                 240 


Lys Glu Phe Lys Glu Glu Ser Arg Thr Met Ser Asn Gly Leu Ala Asn 
                245                 250                 255     


Lys Ser Leu Lys Gly Thr Ile Ser Ala Lys Glu Ala Ala Gly Met Lys 
            260                 265                 270         


Phe Gln Val Trp Asp Tyr Val Pro Leu Asp Val Val Tyr Ser Glu Gly 
        275                 280                 285             


Lys Gln Ser Gly Phe Ala Tyr Asp Val Arg Phe Arg Ala Leu Glu Leu 
    290                 295                 300                 


Met Val Gln Gly Tyr Ser Gln Met Ile Leu Ile Glu Asn His Ile Val 
305                 310                 315                 320 


His Asn Leu Asp Glu Ala Lys Val Ile Tyr Arg Lys Tyr Val Asp Glu 
                325                 330                 335     


Gly Leu Glu Gly Ile Ile Leu Lys Asn Ile Gly Ala Phe Trp Glu Asn 
            340                 345                 350         


Thr Arg Ser Lys Asn Leu Tyr Lys Phe Lys Glu Val Ile Thr Ile Asp 
        355                 360                 365             


Leu Arg Ile Val Asp Ile Tyr Glu His Ser Lys Gln Pro Gly Lys Ala 
    370                 375                 380                 


Gly Gly Phe Tyr Leu Glu Ser Glu Cys Gly Leu Ile Lys Val Lys Ala 
385                 390                 395                 400 


Gly Ser Gly Leu Lys Asp Lys Pro Gly Lys Asp Ala His Glu Leu Asp 
                405                 410                 415     


Arg Thr Arg Ile Trp Glu Asn Lys Asn Asp Tyr Ile Gly Gly Val Leu 
            420                 425                 430         


Glu Ser Glu Cys Asn Gly Trp Leu Ala Ala Glu Gly Arg Thr Asp Tyr 
        435                 440                 445             


Val Lys Leu Phe Leu Pro Ile Ala Ile Lys Met Arg Arg Asp Lys Asp 
    450                 455                 460                 


Val Ala Asn Thr Phe Ala Asp Ile Trp Gly Asp Phe His Glu Val Thr 
465                 470                 475                 480 


Gly Leu 
        


<210>  7
<211>  483
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Wild-type Enterobacteria phage CC31 DNA ligase protein sequence 
       (when fused to CBD)

<400>  7

Gly Ser Ile Leu Asp Ile Ile Asn Glu Ile Ala Ser Ile Gly Ser Thr 
1               5                   10                  15      


Lys Glu Lys Glu Ala Ile Ile Arg Arg His Lys Asp Asn Glu Leu Leu 
            20                  25                  30          


Lys Arg Val Phe Arg Met Thr Tyr Asp Gly Lys Leu Gln Tyr Tyr Ile 
        35                  40                  45              


Lys Lys Trp Asp Thr Arg Pro Lys Gly Asp Ile His Leu Thr Leu Glu 
    50                  55                  60                  


Asp Met Leu Tyr Leu Leu Glu Glu Lys Leu Ala Lys Arg Val Val Thr 
65                  70                  75                  80  


Gly Asn Ala Ala Lys Glu Lys Leu Glu Ile Ala Leu Ser Gln Thr Ser 
                85                  90                  95      


Asp Ala Asp Ala Glu Val Val Lys Lys Val Leu Leu Arg Asp Leu Arg 
            100                 105                 110         


Cys Gly Ala Ser Arg Ser Ile Ala Asn Lys Val Trp Lys Asn Leu Ile 
        115                 120                 125             


Pro Glu Gln Pro Gln Met Leu Ala Ser Ser Tyr Asp Glu Lys Gly Ile 
    130                 135                 140                 


Glu Lys Asn Ile Lys Phe Pro Ala Phe Ala Gln Leu Lys Ala Asp Gly 
145                 150                 155                 160 


Ala Arg Ala Phe Ala Glu Val Arg Gly Asp Glu Leu Asp Asp Val Lys 
                165                 170                 175     


Ile Leu Ser Arg Ala Gly Asn Glu Tyr Leu Gly Leu Asp Leu Leu Lys 
            180                 185                 190         


Gln Gln Leu Ile Glu Met Thr Lys Glu Ala Arg Glu Arg His Pro Gly 
        195                 200                 205             


Gly Val Met Ile Asp Gly Glu Leu Val Tyr His Ala Ser Thr Leu Pro 
    210                 215                 220                 


Ala Gly Pro Leu Asp Asp Ile Phe Gly Asp Leu Pro Glu Leu Ser Lys 
225                 230                 235                 240 


Ala Lys Glu Phe Lys Glu Glu Ser Arg Thr Met Ser Asn Gly Leu Ala 
                245                 250                 255     


Asn Lys Ser Leu Lys Gly Thr Ile Ser Ala Lys Glu Ala Ala Gly Met 
            260                 265                 270         


Lys Phe Gln Val Trp Asp Tyr Val Pro Leu Asp Val Val Tyr Ser Glu 
        275                 280                 285             


Gly Lys Gln Ser Gly Phe Ala Tyr Asp Val Arg Phe Arg Ala Leu Glu 
    290                 295                 300                 


Leu Met Val Gln Gly Tyr Ser Gln Met Ile Leu Ile Glu Asn His Ile 
305                 310                 315                 320 


Val His Asn Leu Asp Glu Ala Lys Val Ile Tyr Arg Lys Tyr Val Asp 
                325                 330                 335     


Glu Gly Leu Glu Gly Ile Ile Leu Lys Asn Ile Gly Ala Phe Trp Glu 
            340                 345                 350         


Asn Thr Arg Ser Lys Asn Leu Tyr Lys Phe Lys Glu Val Ile Thr Ile 
        355                 360                 365             


Asp Leu Arg Ile Val Asp Ile Tyr Glu His Ser Lys Gln Pro Gly Lys 
    370                 375                 380                 


Ala Gly Gly Phe Tyr Leu Glu Ser Glu Cys Gly Leu Ile Lys Val Lys 
385                 390                 395                 400 


Ala Gly Ser Gly Leu Lys Asp Lys Pro Gly Lys Asp Ala His Glu Leu 
                405                 410                 415     


Asp Arg Thr Arg Ile Trp Glu Asn Lys Asn Asp Tyr Ile Gly Gly Val 
            420                 425                 430         


Leu Glu Ser Glu Cys Asn Gly Trp Leu Ala Ala Glu Gly Arg Thr Asp 
        435                 440                 445             


Tyr Val Lys Leu Phe Leu Pro Ile Ala Ile Lys Met Arg Arg Asp Lys 
    450                 455                 460                 


Asp Val Ala Asn Thr Phe Ala Asp Ile Trp Gly Asp Phe His Glu Val 
465                 470                 475                 480 


Thr Gly Leu 
            


<210>  8
<211>  497
<212>  PRT
<213>  Shigella phage Shf125875

<400>  8

Met Ile Leu Asp Ile Leu Asn Gln Ile Ala Ala Ile Gly Ser Thr Lys 
1               5                   10                  15      


Thr Lys Gln Glu Ile Leu Lys Lys Asn Lys Asp Asn Lys Leu Leu Glu 
            20                  25                  30          


Arg Val Tyr Arg Leu Thr Tyr Ala Arg Gly Ile Gln Tyr Tyr Ile Lys 
        35                  40                  45              


Lys Trp Pro Gly Pro Gly Glu Arg Ser Gln Ala Tyr Gly Leu Leu Glu 
    50                  55                  60                  


Leu Asp Asp Met Leu Asp Phe Ile Glu Phe Thr Leu Ala Thr Arg Lys 
65                  70                  75                  80  


Leu Thr Gly Asn Ala Ala Ile Lys Glu Leu Met Gly Tyr Ile Ala Asp 
                85                  90                  95      


Gly Lys Pro Asp Asp Val Glu Val Leu Arg Arg Val Met Met Arg Asp 
            100                 105                 110         


Leu Glu Val Gly Ala Ser Val Ser Ile Ala Asn Lys Val Trp Pro Gly 
        115                 120                 125             


Leu Ile Gln Leu Gln Pro Gln Met Leu Ala Ser Ala Tyr Asp Glu Lys 
    130                 135                 140                 


Leu Ile Thr Lys Asn Ile Lys Trp Pro Ala Phe Ala Gln Leu Lys Ala 
145                 150                 155                 160 


Asp Gly Ala Arg Cys Phe Ala Glu Val Arg Asp Asp Gly Val Gln Phe 
                165                 170                 175     


Phe Ser Arg Ala Gly Asn Glu Tyr His Gly Leu Thr Leu Leu Ala Asp 
            180                 185                 190         


Glu Leu Met Glu Met Thr Lys Glu Ala Arg Glu Arg His Pro Asn Gly 
        195                 200                 205             


Val Leu Ile Asp Gly Glu Leu Val Tyr His Ser Phe Asp Ile Lys Lys 
    210                 215                 220                 


Ala Val Ser Ser Gly Asn Asp Leu Ser Phe Leu Phe Gly Asp Asn Glu 
225                 230                 235                 240 


Glu Ser Glu Glu Val Gln Val Ala Asp Arg Ser Thr Ser Asn Gly Leu 
                245                 250                 255     


Ala Asn Lys Ser Leu Gln Gly Thr Ile Ser Pro Lys Glu Ala Glu Gly 
            260                 265                 270         


Met Val Leu Gln Ala Trp Asp Tyr Val Pro Leu Asp Glu Val Tyr Ser 
        275                 280                 285             


Asp Gly Lys Ile Lys Gly Gln Lys Tyr Asp Val Arg Phe Ala Ala Leu 
    290                 295                 300                 


Glu Asn Met Ala Glu Gly Phe Lys Arg Ile Glu Pro Ile Glu Asn Gln 
305                 310                 315                 320 


Leu Val His Asn Leu Asp Glu Ala Lys Val Val Tyr Lys Lys Tyr Val 
                325                 330                 335     


Asp Gln Gly Leu Glu Gly Ile Ile Leu Lys Asn Arg Asp Ser Tyr Trp 
            340                 345                 350         


Glu Asn Lys Arg Ser Lys Asn Leu Ile Lys Phe Lys Glu Val Ile Asp 
        355                 360                 365             


Ile Ala Leu Glu Val Val Gly Tyr Tyr Glu His Ser Lys Asp Pro Asn 
    370                 375                 380                 


Lys Leu Gly Gly Val Glu Leu Val Ser Arg Cys Arg Arg Ile Thr Thr 
385                 390                 395                 400 


Asp Cys Gly Ser Gly Phe Lys Asp Thr Thr His Lys Thr Val Asp Gly 
                405                 410                 415     


Val Lys Val Leu Ile Pro Leu Asp Glu Arg His Asp Leu Asp Arg Glu 
            420                 425                 430         


Arg Leu Met Ala Glu Ala Arg Glu Gly Lys Leu Ile Gly Arg Ile Ala 
        435                 440                 445             


Asp Cys Glu Cys Asn Gly Trp Val His Ser Lys Gly Arg Glu Gly Thr 
    450                 455                 460                 


Val Gly Ile Phe Leu Pro Ile Ile Lys Gly Phe Arg Phe Asp Lys Thr 
465                 470                 475                 480 


Glu Ala Asp Ser Phe Glu Asp Val Phe Gly Pro Trp Ser Gln Thr Gly 
                485                 490                 495     


Leu 
    


<210>  9
<211>  498
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Wild-type Shigella phage Shf125875 DNA ligase protein sequence 
       (when fused to CBD)

<400>  9

Gly Ser Ile Leu Asp Ile Leu Asn Gln Ile Ala Ala Ile Gly Ser Thr 
1               5                   10                  15      


Lys Thr Lys Gln Glu Ile Leu Lys Lys Asn Lys Asp Asn Lys Leu Leu 
            20                  25                  30          


Glu Arg Val Tyr Arg Leu Thr Tyr Ala Arg Gly Ile Gln Tyr Tyr Ile 
        35                  40                  45              


Lys Lys Trp Pro Gly Pro Gly Glu Arg Ser Gln Ala Tyr Gly Leu Leu 
    50                  55                  60                  


Glu Leu Asp Asp Met Leu Asp Phe Ile Glu Phe Thr Leu Ala Thr Arg 
65                  70                  75                  80  


Lys Leu Thr Gly Asn Ala Ala Ile Lys Glu Leu Met Gly Tyr Ile Ala 
                85                  90                  95      


Asp Gly Lys Pro Asp Asp Val Glu Val Leu Arg Arg Val Met Met Arg 
            100                 105                 110         


Asp Leu Glu Val Gly Ala Ser Val Ser Ile Ala Asn Lys Val Trp Pro 
        115                 120                 125             


Gly Leu Ile Gln Leu Gln Pro Gln Met Leu Ala Ser Ala Tyr Asp Glu 
    130                 135                 140                 


Lys Leu Ile Thr Lys Asn Ile Lys Trp Pro Ala Phe Ala Gln Leu Lys 
145                 150                 155                 160 


Ala Asp Gly Ala Arg Cys Phe Ala Glu Val Arg Asp Asp Gly Val Gln 
                165                 170                 175     


Phe Phe Ser Arg Ala Gly Asn Glu Tyr His Gly Leu Thr Leu Leu Ala 
            180                 185                 190         


Asp Glu Leu Met Glu Met Thr Lys Glu Ala Arg Glu Arg His Pro Asn 
        195                 200                 205             


Gly Val Leu Ile Asp Gly Glu Leu Val Tyr His Ser Phe Asp Ile Lys 
    210                 215                 220                 


Lys Ala Val Ser Ser Gly Asn Asp Leu Ser Phe Leu Phe Gly Asp Asn 
225                 230                 235                 240 


Glu Glu Ser Glu Glu Val Gln Val Ala Asp Arg Ser Thr Ser Asn Gly 
                245                 250                 255     


Leu Ala Asn Lys Ser Leu Gln Gly Thr Ile Ser Pro Lys Glu Ala Glu 
            260                 265                 270         


Gly Met Val Leu Gln Ala Trp Asp Tyr Val Pro Leu Asp Glu Val Tyr 
        275                 280                 285             


Ser Asp Gly Lys Ile Lys Gly Gln Lys Tyr Asp Val Arg Phe Ala Ala 
    290                 295                 300                 


Leu Glu Asn Met Ala Glu Gly Phe Lys Arg Ile Glu Pro Ile Glu Asn 
305                 310                 315                 320 


Gln Leu Val His Asn Leu Asp Glu Ala Lys Val Val Tyr Lys Lys Tyr 
                325                 330                 335     


Val Asp Gln Gly Leu Glu Gly Ile Ile Leu Lys Asn Arg Asp Ser Tyr 
            340                 345                 350         


Trp Glu Asn Lys Arg Ser Lys Asn Leu Ile Lys Phe Lys Glu Val Ile 
        355                 360                 365             


Asp Ile Ala Leu Glu Val Val Gly Tyr Tyr Glu His Ser Lys Asp Pro 
    370                 375                 380                 


Asn Lys Leu Gly Gly Val Glu Leu Val Ser Arg Cys Arg Arg Ile Thr 
385                 390                 395                 400 


Thr Asp Cys Gly Ser Gly Phe Lys Asp Thr Thr His Lys Thr Val Asp 
                405                 410                 415     


Gly Val Lys Val Leu Ile Pro Leu Asp Glu Arg His Asp Leu Asp Arg 
            420                 425                 430         


Glu Arg Leu Met Ala Glu Ala Arg Glu Gly Lys Leu Ile Gly Arg Ile 
        435                 440                 445             


Ala Asp Cys Glu Cys Asn Gly Trp Val His Ser Lys Gly Arg Glu Gly 
    450                 455                 460                 


Thr Val Gly Ile Phe Leu Pro Ile Ile Lys Gly Phe Arg Phe Asp Lys 
465                 470                 475                 480 


Thr Glu Ala Asp Ser Phe Glu Asp Val Phe Gly Pro Trp Ser Gln Thr 
                485                 490                 495     


Gly Leu 
        


<210>  10
<211>  487
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Mutant ligase (T4 backbone) protein sequence

<400>  10

Met Ile Leu Lys Ile Leu Asn Glu Ile Ala Ser Ile Gly Ser Thr Lys 
1               5                   10                  15      


Gln Lys Gln Ala Ile Leu Glu Lys Asn Lys Asp Asn Glu Leu Leu Lys 
            20                  25                  30          


Arg Val Tyr Arg Leu Thr Tyr Ser Arg Gly Leu Gln Tyr Tyr Ile Lys 
        35                  40                  45              


Lys Trp Pro Lys Pro Gly Ile Ala Thr Gln Ser Phe Gly Met Leu Thr 
    50                  55                  60                  


Leu Thr Asp Met Leu Asp Phe Ile Glu Phe Thr Leu Ala Thr Arg Lys 
65                  70                  75                  80  


Leu Thr Gly Asn Ala Ala Ile Glu Glu Leu Thr Gly Tyr Ile Thr Asp 
                85                  90                  95      


Gly Lys Lys Asp Asp Val Glu Val Leu Arg Arg Val Met Met Arg Asp 
            100                 105                 110         


Leu Glu Cys Gly Ala Ser Val Ser Ile Ala Asn Lys Val Trp Pro Gly 
        115                 120                 125             


Leu Ile Pro Glu Gln Pro Gln Met Leu Ala Ser Ser Tyr Asp Glu Lys 
    130                 135                 140                 


Gly Ile Asn Lys Asn Ile Lys Phe Pro Ala Phe Ala Gln Leu Lys Ala 
145                 150                 155                 160 


Asp Gly Ala Arg Cys Phe Ala Glu Val Arg Gly Asp Glu Leu Asp Asp 
                165                 170                 175     


Val Arg Leu Leu Ser Arg Ala Gly Asn Glu Tyr Leu Gly Leu Asp Leu 
            180                 185                 190         


Leu Lys Glu Glu Leu Ile Lys Met Thr Ala Glu Ala Arg Gln Ile His 
        195                 200                 205             


Pro Glu Gly Val Leu Ile Asp Gly Glu Leu Val Tyr His Glu Gln Val 
    210                 215                 220                 


Lys Lys Glu Pro Glu Gly Leu Asp Phe Leu Phe Asp Ala Tyr Pro Glu 
225                 230                 235                 240 


Asn Ser Lys Ala Lys Glu Phe Ala Glu Val Ala Glu Ser Arg Thr Ala 
                245                 250                 255     


Ser Asn Gly Ile Ala Asn Lys Ser Leu Lys Gly Thr Ile Ser Glu Lys 
            260                 265                 270         


Glu Ala Gln Cys Met Lys Phe Gln Val Trp Asp Tyr Val Pro Leu Val 
        275                 280                 285             


Glu Ile Tyr Ser Leu Pro Ala Phe Arg Leu Lys Tyr Asp Val Arg Phe 
    290                 295                 300                 


Ser Lys Leu Glu Gln Met Thr Ser Gly Tyr Asp Lys Val Ile Leu Ile 
305                 310                 315                 320 


Glu Asn Gln Val Val Asn Asn Leu Asp Glu Ala Lys Val Ile Tyr Lys 
                325                 330                 335     


Lys Tyr Ile Asp Gln Gly Leu Glu Gly Ile Ile Leu Lys Asn Ile Asp 
            340                 345                 350         


Gly Leu Trp Glu Asn Ala Arg Ser Lys Asn Leu Tyr Lys Phe Lys Arg 
        355                 360                 365             


Val Ile Asp Val Asp Leu Lys Ile Val Gly Ile Tyr Pro His Arg Lys 
    370                 375                 380                 


Asp Pro Thr Lys Ala Gly Gly Phe Ile Leu Glu Ser Glu Cys Gly Lys 
385                 390                 395                 400 


Ile Lys Val Asn Ala Gly Ser Gly Leu Lys Asp Lys Ala Gly Val Lys 
                405                 410                 415     


Ser His Glu Leu Asp Arg Thr Arg Ile Met Glu Asn Gln Asn Tyr Tyr 
            420                 425                 430         


Ile Gly Lys Ile Leu Glu Cys Glu Cys Asn Gly Trp Leu Lys Ser Asp 
        435                 440                 445             


Gly Arg Thr Asp Tyr Val Lys Leu Phe Leu Pro Ile Ala Ile Arg Leu 
    450                 455                 460                 


Arg Glu Asp Lys Thr Lys Ala Asn Thr Phe Glu Asp Val Phe Gly Asp 
465                 470                 475                 480 


Phe His Glu Val Thr Gly Leu 
                485         


<210>  11
<211>  487
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Mutant ligase (T4 backbone) protein sequence

<400>  11

Met Ile Leu Lys Ile Leu Asn Glu Ile Ala Ser Ile Gly Ser Thr Lys 
1               5                   10                  15      


Gln Lys Gln Ala Ile Leu Glu Lys Asn Lys Asp Asn Glu Leu Leu Lys 
            20                  25                  30          


Arg Val Tyr Arg Leu Thr Tyr Ser Arg Gly Leu Gln Tyr Tyr Ile Lys 
        35                  40                  45              


Lys Trp Pro Lys Pro Gly Ile Ala Thr Gln Ser Phe Gly Met Leu Thr 
    50                  55                  60                  


Leu Thr Asp Met Leu Asp Phe Ile Glu Phe Thr Leu Ala Thr Arg Lys 
65                  70                  75                  80  


Leu Thr Gly Asn Ala Ala Ile Glu Glu Leu Thr Gly Tyr Ile Thr Asp 
                85                  90                  95      


Gly Lys Lys Asp Asp Val Glu Val Leu Arg Arg Val Met Met Arg Asp 
            100                 105                 110         


Leu Glu Cys Gly Ala Ser Val Ser Ile Ala Asn Lys Val Trp Pro Gly 
        115                 120                 125             


Leu Ile Pro Glu Gln Pro Gln Met Leu Ala Ser Ser Tyr Asp Glu Lys 
    130                 135                 140                 


Gly Ile Asn Lys Asn Ile Lys Phe Pro Ala Phe Ala Gln Leu Lys Ala 
145                 150                 155                 160 


Asp Gly Ala Arg Cys Phe Ala Glu Val Arg Gly Asp Glu Leu Asp Asp 
                165                 170                 175     


Val Arg Leu Leu Ser Arg Ala Gly Asn Glu Tyr Leu Gly Leu Asp Leu 
            180                 185                 190         


Leu Lys Glu Glu Leu Ile Lys Met Thr Ala Glu Ala Arg Gln Ile His 
        195                 200                 205             


Pro Glu Gly Val Leu Ile Asp Gly Glu Leu Val Tyr His Glu Gln Val 
    210                 215                 220                 


Lys Lys Glu Pro Glu Gly Leu Asp Phe Leu Phe Asp Ala Tyr Pro Glu 
225                 230                 235                 240 


Asn Ser Lys Ala Lys Glu Phe Ala Glu Val Ala Glu Ser Arg Thr Ala 
                245                 250                 255     


Ser Asn Gly Ile Ala Asn Lys Ser Leu Lys Gly Thr Ile Ser Glu Lys 
            260                 265                 270         


Glu Ala Gln Cys Met Lys Phe Gln Val Trp Asp Tyr Val Pro Leu Val 
        275                 280                 285             


Glu Ile Tyr Ser Leu Pro Ala Phe Arg Leu Lys Tyr Asp Val Arg Phe 
    290                 295                 300                 


Ser Lys Leu Glu Gln Met Thr Ser Gly Tyr Asp Lys Val Ile Leu Ile 
305                 310                 315                 320 


Glu Asn Gln Val Val Asn Asn Leu Asp Glu Ala Lys Val Ile Tyr Lys 
                325                 330                 335     


Lys Tyr Ile Asp Gln Gly Leu Glu Gly Ile Ile Leu Lys Asn Ile Asp 
            340                 345                 350         


Gly Leu Trp Glu Asn Ala Arg Ser Lys Asn Leu Tyr Lys Phe Lys Gly 
        355                 360                 365             


Val Ile Asp Val Asp Leu Lys Ile Val Gly Ile Tyr Pro His Arg Lys 
    370                 375                 380                 


Asp Pro Thr Lys Ala Gly Gly Phe Ile Leu Glu Ser Glu Cys Gly Lys 
385                 390                 395                 400 


Ile Lys Val Asn Ala Gly Ser Gly Leu Lys Asp Lys Ala Gly Val Lys 
                405                 410                 415     


Ser His Glu Leu Asp Arg Thr Arg Ile Met Glu Asn Gln Asn Tyr Tyr 
            420                 425                 430         


Ile Gly Lys Ile Leu Glu Cys Glu Cys Asn Gly Trp Leu Lys Ser Asp 
        435                 440                 445             


Gly Arg Thr Asp Tyr Val Lys Leu Phe Leu Pro Ile Ala Ile Arg Leu 
    450                 455                 460                 


Arg Glu Asp Lys Thr Lys Ala Asn Thr Phe Glu Asp Val Phe Gly Asp 
465                 470                 475                 480 


Phe His Glu Val Thr Gly Leu 
                485         


<210>  12
<211>  487
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Mutant ligase (T4 backbone) protein sequence

<400>  12

Met Ile Leu Lys Ile Leu Asn Glu Ile Ala Ser Ile Gly Ser Thr Lys 
1               5                   10                  15      


Gln Lys Gln Ala Ile Leu Glu Lys Asn Lys Asp Asn Glu Leu Leu Lys 
            20                  25                  30          


Arg Val Tyr Arg Leu Thr Tyr Ser Arg Gly Leu Gln Tyr Tyr Ile Lys 
        35                  40                  45              


Lys Trp Pro Lys Pro Gly Ile Ala Thr Gln Ser Phe Gly Met Leu Thr 
    50                  55                  60                  


Leu Thr Asp Met Leu Asp Phe Ile Glu Phe Thr Leu Ala Thr Arg Lys 
65                  70                  75                  80  


Leu Thr Gly Asn Ala Ala Ile Glu Glu Leu Thr Gly Tyr Ile Thr Asp 
                85                  90                  95      


Gly Lys Lys Asp Asp Val Glu Val Leu Arg Arg Val Met Met Arg Asp 
            100                 105                 110         


Leu Glu Cys Gly Ala Ser Val Ser Ile Ala Asn Lys Val Trp Pro Gly 
        115                 120                 125             


Leu Ile Pro Glu Gln Pro Gln Met Leu Ala Ser Ser Tyr Asp Glu Lys 
    130                 135                 140                 


Gly Ile Asn Lys Asn Ile Lys Phe Pro Ala Phe Ala Gln Leu Lys Ala 
145                 150                 155                 160 


Asp Gly Ala Arg Cys Phe Ala Glu Val Arg Gly Asp Glu Leu Asp Asp 
                165                 170                 175     


Val Arg Leu Leu Ser Arg Ala Gly Asn Glu Tyr Leu Gly Leu Asp Leu 
            180                 185                 190         


Leu Lys Glu Glu Leu Ile Lys Met Thr Ala Glu Ala Arg Gln Ile His 
        195                 200                 205             


Pro Glu Gly Val Leu Ile Asp Gly Glu Leu Val Tyr His Glu Gln Val 
    210                 215                 220                 


Lys Lys Glu Pro Glu Gly Leu Asp Phe Leu Phe Asp Ala Tyr Pro Glu 
225                 230                 235                 240 


Asn Ser Lys Ala Lys Glu Phe Ala Glu Val Ala Glu Ser Arg Thr Ala 
                245                 250                 255     


Ser Asn Gly Ile Ala Asn Lys Ser Leu Lys Gly Thr Ile Ser Glu Lys 
            260                 265                 270         


Glu Ala Gln Cys Met Lys Phe Gln Val Trp Asp Tyr Val Pro Leu Val 
        275                 280                 285             


Glu Ile Tyr Ser Leu Pro Ala Phe Arg Leu Lys Tyr Asp Val Arg Phe 
    290                 295                 300                 


Ser Lys Leu Glu Gln Met Thr Ser Gly Tyr Asp Lys Val Ile Leu Ile 
305                 310                 315                 320 


Glu Asn Gln Val Val Asn Asn Leu Asp Glu Ala Lys Val Ile Tyr Lys 
                325                 330                 335     


Lys Tyr Ile Asp Gln Gly Leu Glu Gly Ile Ile Leu Lys Asn Ile Asp 
            340                 345                 350         


Gly Leu Trp Glu Asn Ala Arg Ser Lys Asn Leu Tyr Lys Phe Lys Lys 
        355                 360                 365             


Val Ile Asp Val Asp Leu Lys Ile Val Gly Ile Tyr Pro His Arg Lys 
    370                 375                 380                 


Asp Pro Thr Lys Ala Gly Gly Phe Ile Leu Glu Ser Glu Cys Gly Lys 
385                 390                 395                 400 


Ile Lys Val Asn Ala Gly Ser Gly Leu Lys Asp Lys Ala Gly Val Lys 
                405                 410                 415     


Ser His Glu Leu Asp Arg Thr Arg Ile Met Glu Asn Gln Asn Tyr Tyr 
            420                 425                 430         


Ile Gly Lys Ile Leu Glu Cys Glu Cys Asn Gly Trp Leu Lys Ser Asp 
        435                 440                 445             


Gly Arg Thr Asp Tyr Val Lys Leu Phe Leu Pro Ile Ala Ile Arg Leu 
    450                 455                 460                 


Arg Glu Asp Lys Thr Lys Ala Asn Thr Phe Glu Asp Val Phe Gly Asp 
465                 470                 475                 480 


Phe His Glu Val Thr Gly Leu 
                485         


<210>  13
<211>  487
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Mutant ligase (T4 backbone) protein sequence

<400>  13

Met Ile Leu Lys Ile Leu Asn Glu Ile Ala Ser Ile Gly Ser Thr Lys 
1               5                   10                  15      


Gln Lys Gln Ala Ile Leu Glu Lys Asn Lys Asp Asn Glu Leu Leu Lys 
            20                  25                  30          


Arg Val Tyr Arg Leu Thr Tyr Ser Arg Gly Leu Gln Tyr Tyr Ile Lys 
        35                  40                  45              


Lys Trp Pro Lys Pro Gly Ile Ala Thr Gln Ser Phe Gly Met Leu Thr 
    50                  55                  60                  


Leu Thr Asp Met Leu Asp Phe Ile Glu Phe Thr Leu Ala Thr Arg Lys 
65                  70                  75                  80  


Leu Thr Gly Asn Ala Ala Ile Glu Glu Leu Thr Gly Tyr Ile Thr Asp 
                85                  90                  95      


Gly Lys Lys Asp Asp Val Glu Val Leu Arg Arg Val Met Met Arg Asp 
            100                 105                 110         


Leu Glu Cys Gly Ala Ser Val Ser Ile Ala Asn Lys Val Trp Pro Gly 
        115                 120                 125             


Leu Ile Pro Glu Gln Pro Gln Met Leu Ala Ser Ser Tyr Asp Glu Lys 
    130                 135                 140                 


Gly Ile Asn Lys Asn Ile Lys Phe Pro Ala Phe Ala Gln Leu Lys Ala 
145                 150                 155                 160 


Asp Gly Ala Arg Cys Phe Ala Glu Val Arg Gly Asp Glu Leu Asp Asp 
                165                 170                 175     


Val Arg Leu Leu Ser Arg Ala Gly Asn Glu Tyr Leu Gly Leu Asp Leu 
            180                 185                 190         


Leu Lys Glu Glu Leu Ile Lys Met Thr Ala Glu Ala Arg Gln Ile His 
        195                 200                 205             


Pro Glu Gly Val Leu Ile Asp Gly Glu Leu Val Tyr His Glu Gln Val 
    210                 215                 220                 


Lys Lys Glu Pro Glu Gly Leu Asp Phe Leu Phe Asp Ala Tyr Pro Glu 
225                 230                 235                 240 


Asn Ser Lys Ala Lys Glu Phe Ala Glu Val Ala Glu Ser Arg Thr Ala 
                245                 250                 255     


Ser Asn Gly Ile Ala Asn Lys Ser Leu Lys Gly Thr Ile Ser Glu Lys 
            260                 265                 270         


Glu Ala Gln Cys Met Lys Phe Gln Val Trp Asp Tyr Val Pro Leu Val 
        275                 280                 285             


Glu Ile Tyr Ser Leu Pro Ala Phe Arg Leu Lys Tyr Asp Val Arg Phe 
    290                 295                 300                 


Ser Lys Leu Glu Gln Met Thr Ser Gly Tyr Asp Lys Val Ile Leu Ile 
305                 310                 315                 320 


Glu Asn Gln Val Val Asn Asn Leu Asp Glu Ala Lys Val Ile Tyr Lys 
                325                 330                 335     


Lys Tyr Ile Asp Gln Gly Leu Glu Gly Ile Ile Leu Lys Asn Ile Asp 
            340                 345                 350         


Gly Leu Trp Glu Asn Ala Arg Ser Lys Asn Leu Tyr Lys Phe Lys Glu 
        355                 360                 365             


Val Ile Leu Val Asp Leu Lys Ile Val Gly Ile Tyr Pro His Arg Lys 
    370                 375                 380                 


Asp Pro Thr Lys Ala Gly Gly Phe Ile Leu Glu Ser Glu Cys Gly Lys 
385                 390                 395                 400 


Ile Lys Val Asn Ala Gly Ser Gly Leu Lys Asp Lys Ala Gly Val Lys 
                405                 410                 415     


Ser His Glu Leu Asp Arg Thr Arg Ile Met Glu Asn Gln Asn Tyr Tyr 
            420                 425                 430         


Ile Gly Lys Ile Leu Glu Cys Glu Cys Asn Gly Trp Leu Lys Ser Asp 
        435                 440                 445             


Gly Arg Thr Asp Tyr Val Lys Leu Phe Leu Pro Ile Ala Ile Arg Leu 
    450                 455                 460                 


Arg Glu Asp Lys Thr Lys Ala Asn Thr Phe Glu Asp Val Phe Gly Asp 
465                 470                 475                 480 


Phe His Glu Val Thr Gly Leu 
                485         


<210>  14
<211>  487
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Mutant ligase (T4 backbone) protein sequence

<400>  14

Met Ile Leu Lys Ile Leu Asn Glu Ile Ala Ser Ile Gly Ser Thr Lys 
1               5                   10                  15      


Gln Lys Gln Ala Ile Leu Glu Lys Asn Lys Asp Asn Glu Leu Leu Lys 
            20                  25                  30          


Arg Val Tyr Arg Leu Thr Tyr Ser Arg Gly Leu Gln Tyr Tyr Ile Lys 
        35                  40                  45              


Lys Trp Pro Lys Pro Gly Ile Ala Thr Gln Ser Phe Gly Met Leu Thr 
    50                  55                  60                  


Leu Thr Asp Met Leu Asp Phe Ile Glu Phe Thr Leu Ala Thr Arg Lys 
65                  70                  75                  80  


Leu Thr Gly Asn Ala Ala Ile Glu Glu Leu Thr Gly Tyr Ile Thr Asp 
                85                  90                  95      


Gly Lys Lys Asp Asp Val Glu Val Leu Arg Arg Val Met Met Arg Asp 
            100                 105                 110         


Leu Glu Cys Gly Ala Ser Val Ser Ile Ala Asn Lys Val Trp Pro Gly 
        115                 120                 125             


Leu Ile Pro Glu Gln Pro Gln Met Leu Ala Ser Ser Tyr Asp Glu Lys 
    130                 135                 140                 


Gly Ile Asn Lys Asn Ile Lys Phe Pro Ala Phe Ala Gln Leu Lys Ala 
145                 150                 155                 160 


Asp Gly Ala Arg Cys Phe Ala Glu Val Arg Gly Asp Glu Leu Asp Asp 
                165                 170                 175     


Val Arg Leu Leu Ser Arg Ala Gly Asn Glu Tyr Leu Gly Leu Asp Leu 
            180                 185                 190         


Leu Lys Glu Glu Leu Ile Lys Met Thr Ala Glu Ala Arg Gln Ile His 
        195                 200                 205             


Pro Glu Gly Val Leu Ile Asp Gly Glu Leu Val Tyr His Glu Gln Val 
    210                 215                 220                 


Lys Lys Glu Pro Glu Gly Leu Asp Phe Leu Phe Asp Ala Tyr Pro Glu 
225                 230                 235                 240 


Asn Ser Lys Ala Lys Glu Phe Ala Glu Val Ala Glu Ser Arg Thr Ala 
                245                 250                 255     


Ser Asn Gly Ile Ala Asn Lys Ser Leu Lys Gly Thr Ile Ser Glu Lys 
            260                 265                 270         


Glu Ala Gln Cys Met Lys Phe Gln Val Trp Asp Tyr Val Pro Leu Val 
        275                 280                 285             


Glu Ile Tyr Ser Leu Pro Ala Phe Arg Leu Lys Tyr Asp Val Arg Phe 
    290                 295                 300                 


Ser Lys Leu Glu Gln Met Thr Ser Gly Tyr Asp Lys Val Ile Leu Ile 
305                 310                 315                 320 


Glu Asn Gln Val Val Asn Asn Leu Asp Glu Ala Lys Val Ile Tyr Lys 
                325                 330                 335     


Lys Tyr Ile Asp Gln Gly Leu Glu Gly Ile Ile Leu Lys Asn Ile Asp 
            340                 345                 350         


Gly Leu Trp Glu Asn Ala Arg Ser Lys Asn Leu Tyr Lys Phe Lys Glu 
        355                 360                 365             


Val Ile Gln Val Asp Leu Lys Ile Val Gly Ile Tyr Pro His Arg Lys 
    370                 375                 380                 


Asp Pro Thr Lys Ala Gly Gly Phe Ile Leu Glu Ser Glu Cys Gly Lys 
385                 390                 395                 400 


Ile Lys Val Asn Ala Gly Ser Gly Leu Lys Asp Lys Ala Gly Val Lys 
                405                 410                 415     


Ser His Glu Leu Asp Arg Thr Arg Ile Met Glu Asn Gln Asn Tyr Tyr 
            420                 425                 430         


Ile Gly Lys Ile Leu Glu Cys Glu Cys Asn Gly Trp Leu Lys Ser Asp 
        435                 440                 445             


Gly Arg Thr Asp Tyr Val Lys Leu Phe Leu Pro Ile Ala Ile Arg Leu 
    450                 455                 460                 


Arg Glu Asp Lys Thr Lys Ala Asn Thr Phe Glu Asp Val Phe Gly Asp 
465                 470                 475                 480 


Phe His Glu Val Thr Gly Leu 
                485         


<210>  15
<211>  487
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Mutant ligase (T4 backbone) protein sequence

<400>  15

Met Ile Leu Lys Ile Leu Asn Glu Ile Ala Ser Ile Gly Ser Thr Lys 
1               5                   10                  15      


Gln Lys Gln Ala Ile Leu Glu Lys Asn Lys Asp Asn Glu Leu Leu Lys 
            20                  25                  30          


Arg Val Tyr Arg Leu Thr Tyr Ser Arg Gly Leu Gln Tyr Tyr Ile Lys 
        35                  40                  45              


Lys Trp Pro Lys Pro Gly Ile Ala Thr Gln Ser Phe Gly Met Leu Thr 
    50                  55                  60                  


Leu Thr Asp Met Leu Asp Phe Ile Glu Phe Thr Leu Ala Thr Arg Lys 
65                  70                  75                  80  


Leu Thr Gly Asn Ala Ala Ile Glu Glu Leu Thr Gly Tyr Ile Thr Asp 
                85                  90                  95      


Gly Lys Lys Asp Asp Val Glu Val Leu Arg Arg Val Met Met Arg Asp 
            100                 105                 110         


Leu Glu Cys Gly Ala Ser Val Ser Ile Ala Asn Lys Val Trp Pro Gly 
        115                 120                 125             


Leu Ile Pro Glu Gln Pro Gln Met Leu Ala Ser Ser Tyr Asp Glu Lys 
    130                 135                 140                 


Gly Ile Asn Lys Asn Ile Lys Phe Pro Ala Phe Ala Gln Leu Lys Ala 
145                 150                 155                 160 


Asp Gly Ala Arg Cys Phe Ala Glu Val Arg Gly Asp Glu Leu Asp Asp 
                165                 170                 175     


Val Arg Leu Leu Ser Arg Ala Gly Asn Glu Tyr Leu Gly Leu Asp Leu 
            180                 185                 190         


Leu Lys Glu Glu Leu Ile Lys Met Thr Ala Glu Ala Arg Gln Ile His 
        195                 200                 205             


Pro Glu Gly Val Leu Ile Asp Gly Glu Leu Val Tyr His Glu Gln Val 
    210                 215                 220                 


Lys Lys Glu Pro Glu Gly Leu Asp Phe Leu Phe Asp Ala Tyr Pro Glu 
225                 230                 235                 240 


Asn Ser Lys Ala Lys Glu Phe Ala Glu Val Ala Glu Ser Arg Thr Ala 
                245                 250                 255     


Ser Asn Gly Ile Ala Asn Lys Ser Leu Lys Gly Thr Ile Ser Glu Lys 
            260                 265                 270         


Glu Ala Gln Cys Met Lys Phe Gln Val Trp Asp Tyr Val Pro Leu Val 
        275                 280                 285             


Glu Ile Tyr Ser Leu Pro Ala Phe Arg Leu Lys Tyr Asp Val Arg Phe 
    290                 295                 300                 


Ser Lys Leu Glu Gln Met Thr Ser Gly Tyr Asp Lys Val Ile Leu Ile 
305                 310                 315                 320 


Glu Asn Gln Val Val Asn Asn Leu Asp Glu Ala Lys Val Ile Tyr Lys 
                325                 330                 335     


Lys Tyr Ile Asp Gln Gly Leu Glu Gly Ile Ile Leu Lys Asn Ile Asp 
            340                 345                 350         


Gly Leu Trp Glu Asn Ala Arg Ser Lys Asn Leu Tyr Lys Phe Lys Glu 
        355                 360                 365             


Val Ile Gln Val Asp Leu Lys Ile Val Gly Ile Tyr Pro His Arg Lys 
    370                 375                 380                 


Asp Pro Thr Lys Ala Gly Gly Phe Ile Leu Glu Ser Glu Cys Gly Lys 
385                 390                 395                 400 


Ile Lys Val Asn Ala Gly Ser Gly Leu Lys Asp Lys Ala Gly Val Lys 
                405                 410                 415     


Ser His Glu Leu Asp Arg Thr Arg Ile Met Glu Asn Gln Asn Tyr Tyr 
            420                 425                 430         


Ile Gly Lys Ile Leu Glu Cys Glu Cys Asn Gly Trp Leu Lys Ser Asp 
        435                 440                 445             


Gly Arg Thr Asp Tyr Val Lys Leu Phe Leu Pro Ile Ala Ile Arg Leu 
    450                 455                 460                 


Arg Glu Asp Lys Thr Lys Ala Asn Thr Phe Glu Asp Val Phe Gly Asp 
465                 470                 475                 480 


Phe His Glu Val Thr Gly Leu 
                485         


<210>  16
<211>  487
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Mutant ligase (T4 backbone) protein sequence

<400>  16

Met Ile Leu Lys Ile Leu Asn Glu Ile Ala Ser Ile Gly Ser Thr Lys 
1               5                   10                  15      


Gln Lys Gln Ala Ile Leu Glu Lys Asn Lys Asp Asn Glu Leu Leu Lys 
            20                  25                  30          


Arg Val Tyr Arg Leu Thr Tyr Ser Arg Gly Leu Gln Tyr Tyr Ile Lys 
        35                  40                  45              


Lys Trp Pro Lys Pro Gly Ile Ala Thr Gln Ser Phe Gly Met Leu Thr 
    50                  55                  60                  


Leu Thr Asp Met Leu Asp Phe Ile Glu Phe Thr Leu Ala Thr Arg Lys 
65                  70                  75                  80  


Leu Thr Gly Asn Ala Ala Ile Glu Glu Leu Thr Gly Tyr Ile Thr Asp 
                85                  90                  95      


Gly Lys Lys Asp Asp Val Glu Val Leu Arg Arg Val Met Met Arg Asp 
            100                 105                 110         


Leu Glu Cys Gly Ala Ser Val Ser Ile Ala Asn Lys Val Trp Pro Gly 
        115                 120                 125             


Leu Ile Pro Glu Gln Pro Gln Met Leu Ala Ser Ser Tyr Asp Glu Lys 
    130                 135                 140                 


Gly Ile Asn Lys Asn Ile Lys Phe Pro Ala Phe Ala Gln Leu Lys Ala 
145                 150                 155                 160 


Asp Gly Ala Arg Cys Phe Ala Glu Val Arg Gly Asp Glu Leu Asp Asp 
                165                 170                 175     


Val Arg Leu Leu Ser Arg Ala Gly Asn Glu Tyr Leu Gly Leu Asp Leu 
            180                 185                 190         


Leu Lys Glu Glu Leu Ile Lys Met Thr Ala Glu Ala Arg Gln Ile His 
        195                 200                 205             


Pro Glu Gly Val Leu Ile Asp Gly Glu Leu Val Tyr His Glu Gln Val 
    210                 215                 220                 


Lys Lys Glu Pro Glu Gly Leu Asp Phe Leu Phe Asp Ala Tyr Pro Glu 
225                 230                 235                 240 


Asn Ser Lys Ala Lys Glu Phe Ala Glu Val Ala Glu Ser Arg Thr Ala 
                245                 250                 255     


Ser Asn Gly Ile Ala Asn Lys Ser Leu Lys Gly Thr Ile Ser Glu Lys 
            260                 265                 270         


Glu Ala Gln Cys Met Lys Phe Gln Val Trp Asp Tyr Val Pro Leu Val 
        275                 280                 285             


Glu Ile Tyr Ser Leu Pro Ala Phe Arg Leu Lys Tyr Asp Val Arg Phe 
    290                 295                 300                 


Ser Lys Leu Glu Gln Met Thr Ser Gly Tyr Asp Lys Val Ile Leu Ile 
305                 310                 315                 320 


Glu Asn Gln Val Val Asn Asn Leu Asp Glu Ala Lys Val Ile Tyr Lys 
                325                 330                 335     


Lys Tyr Ile Asp Gln Gly Leu Glu Gly Ile Ile Leu Lys Asn Ile Asp 
            340                 345                 350         


Gly Leu Trp Glu Asn Ala Arg Ser Lys Asn Leu Tyr Lys Phe Lys Glu 
        355                 360                 365             


Val Ile Val Val Asp Leu Lys Ile Val Gly Ile Tyr Pro His Arg Lys 
    370                 375                 380                 


Asp Pro Thr Lys Ala Gly Gly Phe Ile Leu Glu Ser Glu Cys Gly Lys 
385                 390                 395                 400 


Ile Lys Val Asn Ala Gly Ser Gly Leu Lys Asp Lys Ala Gly Val Lys 
                405                 410                 415     


Ser His Glu Leu Asp Arg Thr Arg Ile Met Glu Asn Gln Asn Tyr Tyr 
            420                 425                 430         


Ile Gly Lys Ile Leu Glu Cys Glu Cys Asn Gly Trp Leu Lys Ser Asp 
        435                 440                 445             


Gly Arg Thr Asp Tyr Val Lys Leu Phe Leu Pro Ile Ala Ile Arg Leu 
    450                 455                 460                 


Arg Glu Asp Lys Thr Lys Ala Asn Thr Phe Glu Asp Val Phe Gly Asp 
465                 470                 475                 480 


Phe His Glu Val Thr Gly Leu 
                485         


<210>  17
<211>  487
<212>  PRT
<213>  Artificial sequence

<220>
<223>   Mutant ligase (T4 backbone) protein sequence

<400>  17

Met Ile Leu Lys Ile Leu Asn Glu Ile Ala Ser Ile Gly Ser Thr Lys 
1               5                   10                  15      


Gln Lys Gln Ala Ile Leu Glu Lys Asn Lys Asp Asn Glu Leu Leu Lys 
            20                  25                  30          


Arg Val Tyr Arg Leu Thr Tyr Ser Arg Gly Leu Gln Tyr Tyr Ile Lys 
        35                  40                  45              


Lys Trp Pro Lys Pro Gly Ile Ala Thr Gln Ser Phe Gly Met Leu Thr 
    50                  55                  60                  


Leu Thr Asp Met Leu Asp Phe Ile Glu Phe Thr Leu Ala Thr Arg Lys 
65                  70                  75                  80  


Leu Thr Gly Asn Ala Ala Ile Glu Glu Leu Thr Gly Tyr Ile Thr Asp 
                85                  90                  95      


Gly Lys Lys Asp Asp Val Glu Val Leu Arg Arg Val Met Met Arg Asp 
            100                 105                 110         


Leu Glu Cys Gly Ala Ser Val Ser Ile Ala Asn Lys Val Trp Pro Gly 
        115                 120                 125             


Leu Ile Pro Glu Gln Pro Gln Met Leu Ala Ser Ser Tyr Asp Glu Lys 
    130                 135                 140                 


Gly Ile Asn Lys Asn Ile Lys Phe Pro Ala Phe Ala Gln Leu Lys Ala 
145                 150                 155                 160 


Asp Gly Ala Arg Cys Phe Ala Glu Val Arg Gly Asp Glu Leu Asp Asp 
                165                 170                 175     


Val Arg Leu Leu Ser Arg Ala Gly Asn Glu Tyr Leu Gly Leu Asp Leu 
            180                 185                 190         


Leu Lys Glu Glu Leu Ile Lys Met Thr Ala Glu Ala Arg Gln Ile His 
        195                 200                 205             


Pro Glu Gly Val Leu Ile Asp Gly Glu Leu Val Tyr His Glu Gln Val 
    210                 215                 220                 


Lys Lys Glu Pro Glu Gly Leu Asp Phe Leu Phe Asp Ala Tyr Pro Glu 
225                 230                 235                 240 


Asn Ser Lys Ala Lys Glu Phe Ala Glu Val Ala Glu Ser Arg Thr Ala 
                245                 250                 255     


Ser Asn Gly Ile Ala Asn Lys Ser Leu Lys Gly Thr Ile Ser Glu Lys 
            260                 265                 270         


Glu Ala Gln Cys Met Lys Phe Gln Val Trp Asp Tyr Val Pro Leu Val 
        275                 280                 285             


Glu Ile Tyr Ser Leu Pro Ala Phe Arg Leu Lys Tyr Asp Val Arg Phe 
    290                 295                 300                 


Ser Lys Leu Glu Gln Met Thr Ser Gly Tyr Asp Lys Val Ile Leu Ile 
305                 310                 315                 320 


Glu Asn Gln Val Val Asn Asn Leu Asp Glu Ala Lys Val Ile Tyr Lys 
                325                 330                 335     


Lys Tyr Ile Asp Gln Gly Leu Glu Gly Ile Ile Leu Lys Asn Ile Asp 
            340                 345                 350         


Gly Leu Trp Glu Asn Ala Arg Ser Lys Asn Leu Tyr Lys Phe Lys Glu 
        355                 360                 365             


Val Ile Arg Val Asp Leu Lys Ile Val Gly Ile Tyr Pro His Arg Lys 
    370                 375                 380                 


Asp Pro Thr Lys Ala Gly Gly Phe Ile Leu Glu Ser Glu Cys Gly Lys 
385                 390                 395                 400 


Ile Lys Val Asn Ala Gly Ser Gly Leu Lys Asp Lys Ala Gly Val Lys 
                405                 410                 415     


Ser His Glu Leu Asp Arg Thr Arg Ile Met Glu Asn Gln Asn Tyr Tyr 
            420                 425                 430         


Ile Gly Lys Ile Leu Glu Cys Glu Cys Asn Gly Trp Leu Lys Ser Asp 
        435                 440                 445             


Gly Arg Thr Asp Tyr Val Lys Leu Phe Leu Pro Ile Ala Ile Arg Leu 
    450                 455                 460                 


Arg Glu Asp Lys Thr Lys Ala Asn Thr Phe Glu Asp Val Phe Gly Asp 
465                 470                 475                 480 


Phe His Glu Val Thr Gly Leu 
                485         


<210>  18
<211>  487
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Mutant ligase (T4 backbone) protein sequence

<400>  18

Met Ile Leu Lys Ile Leu Asn Glu Ile Ala Ser Ile Gly Ser Thr Lys 
1               5                   10                  15      


Gln Lys Gln Ala Ile Leu Glu Lys Asn Lys Asp Asn Glu Leu Leu Lys 
            20                  25                  30          


Arg Val Tyr Arg Leu Thr Tyr Ser Arg Gly Leu Gln Tyr Tyr Ile Lys 
        35                  40                  45              


Lys Trp Pro Lys Pro Gly Ile Ala Thr Gln Ser Phe Gly Met Leu Thr 
    50                  55                  60                  


Leu Thr Asp Met Leu Asp Phe Ile Glu Phe Thr Leu Ala Thr Arg Lys 
65                  70                  75                  80  


Leu Thr Gly Asn Ala Ala Ile Glu Glu Leu Thr Gly Tyr Ile Thr Asp 
                85                  90                  95      


Gly Lys Lys Asp Asp Val Glu Val Leu Arg Arg Val Met Met Arg Asp 
            100                 105                 110         


Leu Glu Cys Gly Ala Ser Val Ser Ile Ala Asn Lys Val Trp Pro Gly 
        115                 120                 125             


Leu Ile Pro Glu Gln Pro Gln Met Leu Ala Ser Ser Tyr Asp Glu Lys 
    130                 135                 140                 


Gly Ile Asn Lys Asn Ile Lys Phe Pro Ala Phe Ala Gln Leu Lys Ala 
145                 150                 155                 160 


Asp Gly Ala Arg Cys Phe Ala Glu Val Arg Gly Asp Glu Leu Asp Asp 
                165                 170                 175     


Val Arg Leu Leu Ser Arg Ala Gly Asn Glu Tyr Leu Gly Leu Asp Leu 
            180                 185                 190         


Leu Lys Glu Glu Leu Ile Lys Met Thr Ala Glu Ala Arg Gln Ile His 
        195                 200                 205             


Pro Glu Gly Val Leu Ile Asp Gly Glu Leu Val Tyr His Glu Gln Val 
    210                 215                 220                 


Lys Lys Glu Pro Glu Gly Leu Asp Phe Leu Phe Asp Ala Tyr Pro Glu 
225                 230                 235                 240 


Asn Ser Lys Ala Lys Glu Phe Ala Glu Val Ala Glu Ser Arg Thr Ala 
                245                 250                 255     


Ser Asn Gly Ile Ala Asn Lys Ser Leu Lys Gly Thr Ile Ser Glu Lys 
            260                 265                 270         


Glu Ala Gln Cys Met Lys Phe Gln Val Trp Asp Tyr Val Pro Leu Val 
        275                 280                 285             


Glu Ile Tyr Ser Leu Pro Ala Phe Arg Leu Lys Tyr Asp Val Arg Phe 
    290                 295                 300                 


Ser Lys Leu Glu Gln Met Thr Ser Gly Tyr Asp Lys Val Ile Leu Ile 
305                 310                 315                 320 


Glu Asn Gln Val Val Asn Asn Leu Asp Glu Ala Lys Val Ile Tyr Lys 
                325                 330                 335     


Lys Tyr Ile Asp Gln Gly Leu Glu Gly Ile Ile Leu Lys Asn Ile Asp 
            340                 345                 350         


Gly Leu Trp Glu Asn Ala Arg Ser Lys Asn Leu Tyr Lys Phe Lys Glu 
        355                 360                 365             


Ala Ile Asp Val Asp Leu Lys Ile Val Gly Ile Tyr Pro His Arg Lys 
    370                 375                 380                 


Asp Pro Thr Lys Ala Gly Gly Phe Ile Leu Glu Ser Glu Cys Gly Lys 
385                 390                 395                 400 


Ile Lys Val Asn Ala Gly Ser Gly Leu Lys Asp Lys Ala Gly Val Lys 
                405                 410                 415     


Ser His Glu Leu Asp Arg Thr Arg Ile Met Glu Asn Gln Asn Tyr Tyr 
            420                 425                 430         


Ile Gly Lys Ile Leu Glu Cys Glu Cys Asn Gly Trp Leu Lys Ser Asp 
        435                 440                 445             


Gly Arg Thr Asp Tyr Val Lys Leu Phe Leu Pro Ile Ala Ile Arg Leu 
    450                 455                 460                 


Arg Glu Asp Lys Thr Lys Ala Asn Thr Phe Glu Asp Val Phe Gly Asp 
465                 470                 475                 480 


Phe His Glu Val Thr Gly Leu 
                485         


<210>  19
<211>  487
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Mutant ligase (T4 backbone) protein sequence

<400>  19

Met Ile Leu Lys Ile Leu Asn Glu Ile Ala Ser Ile Gly Ser Thr Lys 
1               5                   10                  15      


Gln Lys Gln Ala Ile Leu Glu Lys Asn Lys Asp Asn Glu Leu Leu Lys 
            20                  25                  30          


Arg Val Tyr Arg Leu Thr Tyr Ser Arg Gly Leu Gln Tyr Tyr Ile Lys 
        35                  40                  45              


Lys Trp Pro Lys Pro Gly Ile Ala Thr Gln Ser Phe Gly Met Leu Thr 
    50                  55                  60                  


Leu Thr Asp Met Leu Asp Phe Ile Glu Phe Thr Leu Ala Thr Arg Lys 
65                  70                  75                  80  


Leu Thr Gly Asn Ala Ala Ile Glu Glu Leu Thr Gly Tyr Ile Thr Asp 
                85                  90                  95      


Gly Lys Lys Asp Asp Val Glu Val Leu Arg Arg Val Met Met Arg Asp 
            100                 105                 110         


Leu Glu Cys Gly Ala Ser Val Ser Ile Ala Asn Lys Val Trp Pro Gly 
        115                 120                 125             


Leu Ile Pro Glu Gln Pro Gln Met Leu Ala Ser Ser Tyr Asp Glu Lys 
    130                 135                 140                 


Gly Ile Asn Lys Asn Ile Lys Phe Pro Ala Phe Ala Gln Leu Lys Ala 
145                 150                 155                 160 


Asp Gly Ala Arg Cys Phe Ala Glu Val Arg Gly Asp Glu Leu Asp Asp 
                165                 170                 175     


Val Arg Leu Leu Ser Arg Ala Gly Asn Glu Tyr Leu Gly Leu Asp Leu 
            180                 185                 190         


Leu Lys Glu Glu Leu Ile Lys Met Thr Ala Glu Ala Arg Gln Ile His 
        195                 200                 205             


Pro Glu Gly Val Leu Ile Asp Gly Glu Leu Val Tyr His Glu Gln Val 
    210                 215                 220                 


Lys Lys Glu Pro Glu Gly Leu Asp Phe Leu Phe Asp Ala Tyr Pro Glu 
225                 230                 235                 240 


Asn Ser Lys Ala Lys Glu Phe Ala Glu Val Ala Glu Ser Arg Thr Ala 
                245                 250                 255     


Ser Asn Gly Ile Ala Asn Lys Ser Leu Lys Gly Thr Ile Ser Glu Lys 
            260                 265                 270         


Glu Ala Gln Cys Met Lys Phe Gln Val Trp Asp Tyr Val Pro Leu Val 
        275                 280                 285             


Glu Ile Tyr Ser Leu Pro Ala Phe Arg Leu Lys Tyr Asp Val Arg Phe 
    290                 295                 300                 


Ser Lys Leu Glu Gln Met Thr Ser Gly Tyr Asp Lys Val Ile Leu Ile 
305                 310                 315                 320 


Glu Asn Gln Val Val Asn Asn Leu Asp Glu Ala Lys Val Ile Tyr Lys 
                325                 330                 335     


Lys Tyr Ile Asp Gln Gly Leu Glu Gly Ile Ile Leu Lys Asn Ile Asp 
            340                 345                 350         


Gly Leu Trp Glu Asn Ala Arg Ser Lys Asn Leu Tyr Lys Phe Lys Glu 
        355                 360                 365             


Lys Ile Asp Val Asp Leu Lys Ile Val Gly Ile Tyr Pro His Arg Lys 
    370                 375                 380                 


Asp Pro Thr Lys Ala Gly Gly Phe Ile Leu Glu Ser Glu Cys Gly Lys 
385                 390                 395                 400 


Ile Lys Val Asn Ala Gly Ser Gly Leu Lys Asp Lys Ala Gly Val Lys 
                405                 410                 415     


Ser His Glu Leu Asp Arg Thr Arg Ile Met Glu Asn Gln Asn Tyr Tyr 
            420                 425                 430         


Ile Gly Lys Ile Leu Glu Cys Glu Cys Asn Gly Trp Leu Lys Ser Asp 
        435                 440                 445             


Gly Arg Thr Asp Tyr Val Lys Leu Phe Leu Pro Ile Ala Ile Arg Leu 
    450                 455                 460                 


Arg Glu Asp Lys Thr Lys Ala Asn Thr Phe Glu Asp Val Phe Gly Asp 
465                 470                 475                 480 


Phe His Glu Val Thr Gly Leu 
                485         


<210>  20
<211>  487
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Mutant ligase (T4 backbone) protein sequence

<400>  20

Met Ile Leu Lys Ile Leu Asn Glu Ile Ala Ser Ile Gly Ser Thr Lys 
1               5                   10                  15      


Gln Lys Gln Ala Ile Leu Glu Lys Asn Lys Asp Asn Glu Leu Leu Lys 
            20                  25                  30          


Arg Val Tyr Arg Leu Thr Tyr Ser Arg Gly Leu Gln Tyr Tyr Ile Lys 
        35                  40                  45              


Lys Trp Pro Lys Pro Gly Ile Ala Thr Gln Ser Phe Gly Met Leu Thr 
    50                  55                  60                  


Leu Thr Asp Met Leu Asp Phe Ile Glu Phe Thr Leu Ala Thr Arg Lys 
65                  70                  75                  80  


Leu Thr Gly Asn Ala Ala Ile Glu Glu Leu Thr Gly Tyr Ile Thr Asp 
                85                  90                  95      


Gly Lys Lys Asp Asp Val Glu Val Leu Arg Arg Val Met Met Arg Asp 
            100                 105                 110         


Leu Glu Cys Gly Ala Ser Val Ser Ile Ala Asn Lys Val Trp Pro Gly 
        115                 120                 125             


Leu Ile Pro Glu Gln Pro Gln Met Leu Ala Ser Ser Tyr Asp Glu Lys 
    130                 135                 140                 


Gly Ile Asn Lys Asn Ile Lys Phe Pro Ala Phe Ala Gln Leu Lys Ala 
145                 150                 155                 160 


Asp Gly Ala Arg Cys Phe Ala Glu Val Arg Gly Asp Glu Leu Asp Asp 
                165                 170                 175     


Val Arg Leu Leu Ser Arg Ala Gly Asn Glu Tyr Leu Gly Leu Asp Leu 
            180                 185                 190         


Leu Lys Glu Glu Leu Ile Lys Met Thr Ala Glu Ala Arg Gln Ile His 
        195                 200                 205             


Pro Glu Gly Val Leu Ile Asp Gly Glu Leu Val Tyr His Glu Gln Val 
    210                 215                 220                 


Lys Lys Glu Pro Glu Gly Leu Asp Phe Leu Phe Asp Ala Tyr Pro Glu 
225                 230                 235                 240 


Asn Ser Lys Ala Lys Glu Phe Ala Glu Val Ala Glu Ser Arg Thr Ala 
                245                 250                 255     


Ser Asn Gly Ile Ala Asn Lys Ser Leu Lys Gly Thr Ile Ser Glu Lys 
            260                 265                 270         


Glu Ala Gln Cys Met Lys Phe Gln Val Trp Asp Tyr Val Pro Leu Val 
        275                 280                 285             


Glu Ile Tyr Ser Leu Pro Ala Phe Arg Leu Lys Tyr Asp Val Arg Phe 
    290                 295                 300                 


Ser Lys Leu Glu Gln Met Thr Ser Gly Tyr Asp Lys Val Ile Leu Ile 
305                 310                 315                 320 


Glu Asn Gln Val Val Asn Asn Leu Asp Glu Ala Lys Val Ile Tyr Lys 
                325                 330                 335     


Lys Tyr Ile Asp Gln Gly Leu Glu Gly Ile Ile Leu Lys Asn Ile Asp 
            340                 345                 350         


Gly Leu Trp Glu Asn Ala Arg Ser Lys Asn Leu Tyr Lys Phe Lys Arg 
        355                 360                 365             


Val Ile Val Val Asp Leu Lys Ile Val Gly Ile Tyr Pro His Arg Lys 
    370                 375                 380                 


Asp Pro Thr Lys Ala Gly Gly Phe Ile Leu Glu Ser Glu Cys Gly Lys 
385                 390                 395                 400 


Ile Lys Val Asn Ala Gly Ser Gly Leu Lys Asp Lys Ala Gly Val Lys 
                405                 410                 415     


Ser His Glu Leu Asp Arg Thr Arg Ile Met Glu Asn Gln Asn Tyr Tyr 
            420                 425                 430         


Ile Gly Lys Ile Leu Glu Cys Glu Cys Asn Gly Trp Leu Lys Ser Asp 
        435                 440                 445             


Gly Arg Thr Asp Tyr Val Lys Leu Phe Leu Pro Ile Ala Ile Arg Leu 
    450                 455                 460                 


Arg Glu Asp Lys Thr Lys Ala Asn Thr Phe Glu Asp Val Phe Gly Asp 
465                 470                 475                 480 


Phe His Glu Val Thr Gly Leu 
                485         


<210>  21
<211>  487
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Mutant ligase (T4 backbone) protein sequence

<400>  21

Met Ile Leu Lys Ile Leu Asn Glu Ile Ala Ser Ile Gly Ser Thr Lys 
1               5                   10                  15      


Gln Lys Gln Ala Ile Leu Glu Lys Asn Lys Asp Asn Glu Leu Leu Lys 
            20                  25                  30          


Arg Val Tyr Arg Leu Thr Tyr Ser Arg Gly Leu Gln Tyr Tyr Ile Lys 
        35                  40                  45              


Lys Trp Pro Lys Pro Gly Ile Ala Thr Gln Ser Phe Gly Met Leu Thr 
    50                  55                  60                  


Leu Thr Asp Met Leu Asp Phe Ile Glu Phe Thr Leu Ala Thr Arg Lys 
65                  70                  75                  80  


Leu Thr Gly Asn Ala Ala Ile Glu Glu Leu Thr Gly Tyr Ile Thr Asp 
                85                  90                  95      


Gly Lys Lys Asp Asp Val Glu Val Leu Arg Arg Val Met Met Arg Asp 
            100                 105                 110         


Leu Glu Cys Gly Ala Ser Val Ser Ile Ala Asn Lys Val Trp Pro Gly 
        115                 120                 125             


Leu Ile Pro Glu Gln Pro Gln Met Leu Ala Ser Ser Tyr Asp Glu Lys 
    130                 135                 140                 


Gly Ile Asn Lys Asn Ile Lys Phe Pro Ala Phe Ala Gln Leu Lys Ala 
145                 150                 155                 160 


Asp Gly Ala Arg Cys Phe Ala Glu Val Arg Gly Asp Glu Leu Asp Asp 
                165                 170                 175     


Val Arg Leu Leu Ser Arg Ala Gly Asn Glu Tyr Leu Gly Leu Asp Leu 
            180                 185                 190         


Leu Lys Glu Glu Leu Ile Lys Met Thr Ala Glu Ala Arg Gln Ile His 
        195                 200                 205             


Pro Glu Gly Val Leu Ile Asp Gly Glu Leu Val Tyr His Glu Gln Val 
    210                 215                 220                 


Lys Lys Glu Pro Glu Gly Leu Asp Phe Leu Phe Asp Ala Tyr Pro Glu 
225                 230                 235                 240 


Asn Ser Lys Ala Lys Glu Phe Ala Glu Val Ala Glu Ser Arg Thr Ala 
                245                 250                 255     


Ser Asn Gly Ile Ala Asn Lys Ser Leu Lys Gly Thr Ile Ser Glu Lys 
            260                 265                 270         


Glu Ala Gln Cys Met Lys Phe Gln Val Trp Asp Tyr Val Pro Leu Val 
        275                 280                 285             


Glu Ile Tyr Ser Leu Pro Ala Phe Arg Leu Lys Tyr Asp Val Arg Phe 
    290                 295                 300                 


Ser Lys Leu Glu Gln Met Thr Ser Gly Tyr Asp Lys Val Ile Leu Ile 
305                 310                 315                 320 


Glu Asn Gln Val Val Asn Asn Leu Asp Glu Ala Lys Val Ile Tyr Lys 
                325                 330                 335     


Lys Tyr Ile Asp Gln Gly Leu Glu Gly Ile Ile Leu Lys Asn Ile Asp 
            340                 345                 350         


Gly Leu Trp Glu Asn Ala Arg Ser Lys Asn Leu Tyr Lys Phe Lys Lys 
        355                 360                 365             


Val Ile Glu Val Asp Leu Lys Ile Val Gly Ile Tyr Pro His Arg Lys 
    370                 375                 380                 


Asp Pro Thr Lys Ala Gly Gly Phe Ile Leu Glu Ser Glu Cys Gly Lys 
385                 390                 395                 400 


Ile Lys Val Asn Ala Gly Ser Gly Leu Lys Asp Lys Ala Gly Val Lys 
                405                 410                 415     


Ser His Glu Leu Asp Arg Thr Arg Ile Met Glu Asn Gln Asn Tyr Tyr 
            420                 425                 430         


Ile Gly Lys Ile Leu Glu Cys Glu Cys Asn Gly Trp Leu Lys Ser Asp 
        435                 440                 445             


Gly Arg Thr Asp Tyr Val Lys Leu Phe Leu Pro Ile Ala Ile Arg Leu 
    450                 455                 460                 


Arg Glu Asp Lys Thr Lys Ala Asn Thr Phe Glu Asp Val Phe Gly Asp 
465                 470                 475                 480 


Phe His Glu Val Thr Gly Leu 
                485         


<210>  22
<211>  487
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Mutant ligase (T4 backbone) protein sequence

<400>  22

Met Ile Leu Lys Ile Leu Asn Glu Ile Ala Ser Ile Gly Ser Thr Lys 
1               5                   10                  15      


Gln Lys Gln Ala Ile Leu Glu Lys Asn Lys Asp Asn Glu Leu Leu Lys 
            20                  25                  30          


Arg Val Tyr Arg Leu Thr Tyr Ser Arg Gly Leu Gln Tyr Tyr Ile Lys 
        35                  40                  45              


Lys Trp Pro Lys Pro Gly Ile Ala Thr Gln Ser Phe Gly Met Leu Thr 
    50                  55                  60                  


Leu Thr Asp Met Leu Asp Phe Ile Glu Phe Thr Leu Ala Thr Arg Lys 
65                  70                  75                  80  


Leu Thr Gly Asn Ala Ala Ile Glu Glu Leu Thr Gly Tyr Ile Thr Asp 
                85                  90                  95      


Gly Lys Lys Asp Asp Val Glu Val Leu Arg Arg Val Met Met Arg Asp 
            100                 105                 110         


Leu Glu Cys Gly Ala Ser Val Ser Ile Ala Asn Lys Val Trp Pro Gly 
        115                 120                 125             


Leu Ile Pro Glu Gln Pro Gln Met Leu Ala Ser Ser Tyr Asp Glu Lys 
    130                 135                 140                 


Gly Ile Asn Lys Asn Ile Lys Phe Pro Ala Phe Ala Gln Leu Lys Ala 
145                 150                 155                 160 


Asp Gly Ala Arg Cys Phe Ala Glu Val Arg Gly Asp Glu Leu Asp Asp 
                165                 170                 175     


Val Arg Leu Leu Ser Arg Ala Gly Asn Glu Tyr Leu Gly Leu Asp Leu 
            180                 185                 190         


Leu Lys Glu Glu Leu Ile Lys Met Thr Ala Glu Ala Arg Gln Ile His 
        195                 200                 205             


Pro Glu Gly Val Leu Ile Asp Gly Glu Leu Val Tyr His Glu Gln Val 
    210                 215                 220                 


Lys Lys Glu Pro Glu Gly Leu Asp Phe Leu Phe Asp Ala Tyr Pro Glu 
225                 230                 235                 240 


Asn Ser Lys Ala Lys Glu Phe Ala Glu Val Ala Glu Ser Arg Thr Ala 
                245                 250                 255     


Ser Asn Gly Ile Ala Asn Lys Ser Leu Lys Gly Thr Ile Ser Glu Lys 
            260                 265                 270         


Glu Ala Gln Cys Met Lys Phe Gln Val Trp Asp Tyr Val Pro Leu Val 
        275                 280                 285             


Glu Ile Tyr Ser Leu Pro Ala Phe Arg Leu Lys Tyr Asp Val Arg Phe 
    290                 295                 300                 


Ser Lys Leu Glu Gln Met Thr Ser Gly Tyr Asp Lys Val Ile Leu Ile 
305                 310                 315                 320 


Glu Asn Gln Val Val Asn Asn Leu Asp Glu Ala Lys Val Ile Tyr Lys 
                325                 330                 335     


Lys Tyr Ile Asp Gln Gly Leu Glu Gly Ile Ile Leu Lys Asn Ile Asp 
            340                 345                 350         


Gly Leu Trp Glu Asn Ala Arg Ser Lys Asn Leu Tyr Lys Phe Lys Lys 
        355                 360                 365             


Val Ile His Val Asp Leu Lys Ile Val Gly Ile Tyr Pro His Arg Lys 
    370                 375                 380                 


Asp Pro Thr Lys Ala Gly Gly Phe Ile Leu Glu Ser Glu Cys Gly Lys 
385                 390                 395                 400 


Ile Lys Val Asn Ala Gly Ser Gly Leu Lys Asp Lys Ala Gly Val Lys 
                405                 410                 415     


Ser His Glu Leu Asp Arg Thr Arg Ile Met Glu Asn Gln Asn Tyr Tyr 
            420                 425                 430         


Ile Gly Lys Ile Leu Glu Cys Glu Cys Asn Gly Trp Leu Lys Ser Asp 
        435                 440                 445             


Gly Arg Thr Asp Tyr Val Lys Leu Phe Leu Pro Ile Ala Ile Arg Leu 
    450                 455                 460                 


Arg Glu Asp Lys Thr Lys Ala Asn Thr Phe Glu Asp Val Phe Gly Asp 
465                 470                 475                 480 


Phe His Glu Val Thr Gly Leu 
                485         


<210>  23
<211>  482
<212>  PRT
<213>  Artificial sequence

<220>
<223>   Mutant ligase (Enterobacteria phage CC31 backbone - clone A4) 
       protein sequence

<400>  23

Met Ile Leu Asp Ile Ile Asn Glu Ile Ala Ser Ile Gly Ser Thr Lys 
1               5                   10                  15      


Glu Lys Glu Ala Ile Ile Arg Arg His Lys Asp Asn Glu Leu Leu Lys 
            20                  25                  30          


Arg Val Phe Arg Met Thr Tyr Asp Gly Lys Leu Gln Tyr Tyr Ile Lys 
        35                  40                  45              


Lys Trp Asp Thr Arg Pro Lys Gly Asp Ile His Leu Thr Leu Glu Asp 
    50                  55                  60                  


Met Leu Tyr Leu Leu Glu Glu Lys Leu Ala Lys Arg Val Val Thr Gly 
65                  70                  75                  80  


Asn Ala Ala Lys Glu Lys Leu Glu Ile Ala Leu Ser Gln Thr Ser Asp 
                85                  90                  95      


Ala Asp Ala Glu Val Val Lys Lys Val Leu Leu Arg Asp Leu Arg Cys 
            100                 105                 110         


Gly Ala Ser Arg Ser Ile Ala Asn Lys Val Trp Lys Asn Leu Ile Pro 
        115                 120                 125             


Glu Gln Pro Gln Met Leu Ala Ser Ser Tyr Asp Glu Lys Gly Ile Glu 
    130                 135                 140                 


Lys Asn Ile Lys Phe Pro Ala Phe Ala Gln Leu Lys Ala Asp Gly Ala 
145                 150                 155                 160 


Arg Ala Phe Ala Glu Val Arg Gly Asp Glu Leu Asp Asp Val Lys Ile 
                165                 170                 175     


Leu Ser Arg Ala Gly Asn Glu Tyr Leu Gly Leu Asp Leu Leu Lys Gln 
            180                 185                 190         


Gln Leu Ile Glu Met Thr Lys Glu Ala Arg Glu Arg His Pro Gly Gly 
        195                 200                 205             


Val Met Ile Asp Gly Glu Leu Val Tyr His Ala Ser Thr Leu Pro Ala 
    210                 215                 220                 


Gly Pro Leu Asp Asp Ile Phe Gly Asp Leu Pro Glu Leu Ser Lys Ala 
225                 230                 235                 240 


Lys Glu Phe Lys Glu Glu Ser Arg Thr Met Ser Asn Gly Leu Ala Asn 
                245                 250                 255     


Lys Ser Leu Lys Gly Thr Ile Ser Ala Lys Glu Ala Ala Gly Met Lys 
            260                 265                 270         


Phe Gln Val Trp Asp Tyr Val Pro Leu Asp Val Val Tyr Ser Glu Gly 
        275                 280                 285             


Lys Gln Ser Gly Phe Ala Tyr Asp Val Arg Phe Arg Ala Leu Glu Leu 
    290                 295                 300                 


Met Val Gln Gly Tyr Ser Gln Met Ile Leu Ile Glu Asn His Ile Val 
305                 310                 315                 320 


His Asn Leu Asp Glu Ala Lys Val Ile Tyr Arg Lys Tyr Val Asp Glu 
                325                 330                 335     


Gly Leu Glu Gly Ile Ile Leu Lys Asn Ile Gly Ala Phe Trp Glu Asn 
            340                 345                 350         


Thr Arg Ser Lys Asn Leu Tyr Lys Phe Lys Arg Val Ile Val Ile Asp 
        355                 360                 365             


Leu Arg Ile Val Asp Ile Tyr Glu His Ser Lys Gln Pro Gly Lys Ala 
    370                 375                 380                 


Gly Gly Phe Tyr Leu Glu Ser Glu Cys Gly Leu Ile Lys Val Lys Ala 
385                 390                 395                 400 


Gly Ser Gly Leu Lys Asp Lys Pro Gly Lys Asp Ala His Glu Leu Asp 
                405                 410                 415     


Arg Thr Arg Ile Trp Glu Asn Lys Asn Asp Tyr Ile Gly Gly Val Leu 
            420                 425                 430         


Glu Ser Glu Cys Asn Gly Trp Leu Ala Ala Glu Gly Arg Thr Asp Tyr 
        435                 440                 445             


Val Lys Leu Phe Leu Pro Ile Ala Ile Lys Met Arg Arg Asp Lys Asp 
    450                 455                 460                 


Val Ala Asn Thr Phe Ala Asp Ile Trp Gly Asp Phe His Glu Val Thr 
465                 470                 475                 480 


Gly Leu 
        


<210>  24
<211>  482
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Mutant ligase (Enterobacteria phage CC31 backbone) protein 
       sequence

<400>  24

Met Ile Leu Asp Ile Ile Asn Glu Ile Ala Ser Ile Gly Ser Thr Lys 
1               5                   10                  15      


Glu Lys Glu Ala Ile Ile Arg Arg His Lys Asp Asn Glu Leu Leu Lys 
            20                  25                  30          


Arg Val Phe Arg Met Thr Tyr Asp Gly Lys Leu Gln Tyr Tyr Ile Lys 
        35                  40                  45              


Lys Trp Asp Thr Arg Pro Lys Gly Asp Ile His Leu Thr Leu Glu Asp 
    50                  55                  60                  


Met Leu Tyr Leu Leu Glu Glu Lys Leu Ala Lys Arg Val Val Thr Gly 
65                  70                  75                  80  


Asn Ala Ala Lys Glu Lys Leu Glu Ile Ala Leu Ser Gln Thr Ser Asp 
                85                  90                  95      


Ala Asp Ala Glu Val Val Lys Lys Val Leu Leu Arg Asp Leu Arg Cys 
            100                 105                 110         


Gly Ala Ser Arg Ser Ile Ala Asn Lys Val Trp Lys Asn Leu Ile Pro 
        115                 120                 125             


Glu Gln Pro Gln Met Leu Ala Ser Ser Tyr Asp Glu Lys Gly Ile Glu 
    130                 135                 140                 


Lys Asn Ile Lys Phe Pro Ala Phe Ala Gln Leu Lys Ala Asp Gly Ala 
145                 150                 155                 160 


Arg Ala Phe Ala Glu Val Arg Gly Asp Glu Leu Asp Asp Val Lys Ile 
                165                 170                 175     


Leu Ser Arg Ala Gly Asn Glu Tyr Leu Gly Leu Asp Leu Leu Lys Gln 
            180                 185                 190         


Gln Leu Ile Glu Met Thr Lys Glu Ala Arg Glu Arg His Pro Gly Gly 
        195                 200                 205             


Val Met Ile Asp Gly Glu Leu Val Tyr His Ala Ser Thr Leu Pro Ala 
    210                 215                 220                 


Gly Pro Leu Asp Asp Ile Phe Gly Asp Leu Pro Glu Leu Ser Lys Ala 
225                 230                 235                 240 


Lys Glu Phe Lys Glu Glu Ser Arg Thr Met Ser Asn Gly Leu Ala Asn 
                245                 250                 255     


Lys Ser Leu Lys Gly Thr Ile Ser Ala Lys Glu Ala Ala Gly Met Lys 
            260                 265                 270         


Phe Gln Val Trp Asp Tyr Val Pro Leu Asp Val Val Tyr Ser Glu Gly 
        275                 280                 285             


Lys Gln Ser Gly Phe Ala Tyr Asp Val Arg Phe Arg Ala Leu Glu Leu 
    290                 295                 300                 


Met Val Gln Gly Tyr Ser Gln Met Ile Leu Ile Glu Asn His Ile Val 
305                 310                 315                 320 


His Asn Leu Asp Glu Ala Lys Val Ile Tyr Arg Lys Tyr Val Asp Glu 
                325                 330                 335     


Gly Leu Glu Gly Ile Ile Leu Lys Asn Ile Gly Ala Phe Trp Glu Asn 
            340                 345                 350         


Thr Arg Ser Lys Asn Leu Tyr Lys Phe Lys Lys Val Ile Lys Ile Asp 
        355                 360                 365             


Leu Arg Ile Val Asp Ile Tyr Glu His Ser Lys Gln Pro Gly Lys Ala 
    370                 375                 380                 


Gly Gly Phe Tyr Leu Glu Ser Glu Cys Gly Leu Ile Lys Val Lys Ala 
385                 390                 395                 400 


Gly Ser Gly Leu Lys Asp Lys Pro Gly Lys Asp Ala His Glu Leu Asp 
                405                 410                 415     


Arg Thr Arg Ile Trp Glu Asn Lys Asn Asp Tyr Ile Gly Gly Val Leu 
            420                 425                 430         


Glu Ser Glu Cys Asn Gly Trp Leu Ala Ala Glu Gly Arg Thr Asp Tyr 
        435                 440                 445             


Val Lys Leu Phe Leu Pro Ile Ala Ile Lys Met Arg Arg Asp Lys Asp 
    450                 455                 460                 


Val Ala Asn Thr Phe Ala Asp Ile Trp Gly Asp Phe His Glu Val Thr 
465                 470                 475                 480 


Gly Leu 
        


<210>  25
<211>  482
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Mutant ligase (Enterobacteria phage CC31 backbone) protein 
       sequence

<400>  25

Met Ile Leu Asp Ile Ile Asn Glu Ile Ala Ser Ile Gly Ser Thr Lys 
1               5                   10                  15      


Glu Lys Glu Ala Ile Ile Arg Arg His Lys Asp Asn Glu Leu Leu Lys 
            20                  25                  30          


Arg Val Phe Arg Met Thr Tyr Asp Gly Lys Leu Gln Tyr Tyr Ile Lys 
        35                  40                  45              


Lys Trp Asp Thr Arg Pro Lys Gly Asp Ile His Leu Thr Leu Glu Asp 
    50                  55                  60                  


Met Leu Tyr Leu Leu Glu Glu Lys Leu Ala Lys Arg Val Val Thr Gly 
65                  70                  75                  80  


Asn Ala Ala Lys Glu Lys Leu Glu Ile Ala Leu Ser Gln Thr Ser Asp 
                85                  90                  95      


Ala Asp Ala Glu Val Val Lys Lys Val Leu Leu Arg Asp Leu Arg Cys 
            100                 105                 110         


Gly Ala Ser Arg Ser Ile Ala Asn Lys Val Trp Lys Asn Leu Ile Pro 
        115                 120                 125             


Glu Gln Pro Gln Met Leu Ala Ser Ser Tyr Asp Glu Lys Gly Ile Glu 
    130                 135                 140                 


Lys Asn Ile Lys Phe Pro Ala Phe Ala Gln Leu Lys Ala Asp Gly Ala 
145                 150                 155                 160 


Arg Ala Phe Ala Glu Val Arg Gly Asp Glu Leu Asp Asp Val Lys Ile 
                165                 170                 175     


Leu Ser Arg Ala Gly Asn Glu Tyr Leu Gly Leu Asp Leu Leu Lys Gln 
            180                 185                 190         


Gln Leu Ile Glu Met Thr Lys Glu Ala Arg Glu Arg His Pro Gly Gly 
        195                 200                 205             


Val Met Ile Asp Gly Glu Leu Val Tyr His Ala Ser Thr Leu Pro Ala 
    210                 215                 220                 


Gly Pro Leu Asp Asp Ile Phe Gly Asp Leu Pro Glu Leu Ser Lys Ala 
225                 230                 235                 240 


Lys Glu Phe Lys Glu Glu Ser Arg Thr Met Ser Asn Gly Leu Ala Asn 
                245                 250                 255     


Lys Ser Leu Lys Gly Thr Ile Ser Ala Lys Glu Ala Ala Gly Met Lys 
            260                 265                 270         


Phe Gln Val Trp Asp Tyr Val Pro Leu Asp Val Val Tyr Ser Glu Gly 
        275                 280                 285             


Lys Gln Ser Gly Phe Ala Tyr Asp Val Arg Phe Arg Ala Leu Glu Leu 
    290                 295                 300                 


Met Val Gln Gly Tyr Ser Gln Met Ile Leu Ile Glu Asn His Ile Val 
305                 310                 315                 320 


His Asn Leu Asp Glu Ala Lys Val Ile Tyr Arg Lys Tyr Val Asp Glu 
                325                 330                 335     


Gly Leu Glu Gly Ile Ile Leu Lys Asn Ile Gly Ala Phe Trp Glu Asn 
            340                 345                 350         


Thr Arg Ser Lys Asn Leu Tyr Lys Phe Lys Gly Val Ile Phe Ile Asp 
        355                 360                 365             


Leu Arg Ile Val Asp Ile Tyr Glu His Ser Lys Gln Pro Gly Lys Ala 
    370                 375                 380                 


Gly Gly Phe Tyr Leu Glu Ser Glu Cys Gly Leu Ile Lys Val Lys Ala 
385                 390                 395                 400 


Gly Ser Gly Leu Lys Asp Lys Pro Gly Lys Asp Ala His Glu Leu Asp 
                405                 410                 415     


Arg Thr Arg Ile Trp Glu Asn Lys Asn Asp Tyr Ile Gly Gly Val Leu 
            420                 425                 430         


Glu Ser Glu Cys Asn Gly Trp Leu Ala Ala Glu Gly Arg Thr Asp Tyr 
        435                 440                 445             


Val Lys Leu Phe Leu Pro Ile Ala Ile Lys Met Arg Arg Asp Lys Asp 
    450                 455                 460                 


Val Ala Asn Thr Phe Ala Asp Ile Trp Gly Asp Phe His Glu Val Thr 
465                 470                 475                 480 


Gly Leu 
        


<210>  26
<211>  482
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Mutant ligase (Enterobacteria phage CC31 backbone) protein 
       sequence

<400>  26

Met Ile Leu Asp Ile Ile Asn Glu Ile Ala Ser Ile Gly Ser Thr Lys 
1               5                   10                  15      


Glu Lys Glu Ala Ile Ile Arg Arg His Lys Asp Asn Glu Leu Leu Lys 
            20                  25                  30          


Arg Val Phe Arg Met Thr Tyr Asp Gly Lys Leu Gln Tyr Tyr Ile Lys 
        35                  40                  45              


Lys Trp Asp Thr Arg Pro Lys Gly Asp Ile His Leu Thr Leu Glu Asp 
    50                  55                  60                  


Met Leu Tyr Leu Leu Glu Glu Lys Leu Ala Lys Arg Val Val Thr Gly 
65                  70                  75                  80  


Asn Ala Ala Lys Glu Lys Leu Glu Ile Ala Leu Ser Gln Thr Ser Asp 
                85                  90                  95      


Ala Asp Ala Glu Val Val Lys Lys Val Leu Leu Arg Asp Leu Arg Cys 
            100                 105                 110         


Gly Ala Ser Arg Ser Ile Ala Asn Lys Val Trp Lys Asn Leu Ile Pro 
        115                 120                 125             


Glu Gln Pro Gln Met Leu Ala Ser Ser Tyr Asp Glu Lys Gly Ile Glu 
    130                 135                 140                 


Lys Asn Ile Lys Phe Pro Ala Phe Ala Gln Leu Lys Ala Asp Gly Ala 
145                 150                 155                 160 


Arg Ala Phe Ala Glu Val Arg Gly Asp Glu Leu Asp Asp Val Lys Ile 
                165                 170                 175     


Leu Ser Arg Ala Gly Asn Glu Tyr Leu Gly Leu Asp Leu Leu Lys Gln 
            180                 185                 190         


Gln Leu Ile Glu Met Thr Lys Glu Ala Arg Glu Arg His Pro Gly Gly 
        195                 200                 205             


Val Met Ile Asp Gly Glu Leu Val Tyr His Ala Ser Thr Leu Pro Ala 
    210                 215                 220                 


Gly Pro Leu Asp Asp Ile Phe Gly Asp Leu Pro Glu Leu Ser Lys Ala 
225                 230                 235                 240 


Lys Glu Phe Lys Glu Glu Ser Arg Thr Met Ser Asn Gly Leu Ala Asn 
                245                 250                 255     


Lys Ser Leu Lys Gly Thr Ile Ser Ala Lys Glu Ala Ala Gly Met Lys 
            260                 265                 270         


Phe Gln Val Trp Asp Tyr Val Pro Leu Asp Val Val Tyr Ser Glu Gly 
        275                 280                 285             


Lys Gln Ser Gly Phe Ala Tyr Asp Val Arg Phe Arg Ala Leu Glu Leu 
    290                 295                 300                 


Met Val Gln Gly Tyr Ser Gln Met Ile Leu Ile Glu Asn His Ile Val 
305                 310                 315                 320 


His Asn Leu Asp Glu Ala Lys Val Ile Tyr Arg Lys Tyr Val Asp Glu 
                325                 330                 335     


Gly Leu Glu Gly Ile Ile Leu Lys Asn Ile Gly Ala Phe Trp Glu Asn 
            340                 345                 350         


Thr Arg Ser Lys Asn Leu Tyr Lys Phe Lys Gly Val Ile Leu Ile Asp 
        355                 360                 365             


Leu Arg Ile Val Asp Ile Tyr Glu His Ser Lys Gln Pro Gly Lys Ala 
    370                 375                 380                 


Gly Gly Phe Tyr Leu Glu Ser Glu Cys Gly Leu Ile Lys Val Lys Ala 
385                 390                 395                 400 


Gly Ser Gly Leu Lys Asp Lys Pro Gly Lys Asp Ala His Glu Leu Asp 
                405                 410                 415     


Arg Thr Arg Ile Trp Glu Asn Lys Asn Asp Tyr Ile Gly Gly Val Leu 
            420                 425                 430         


Glu Ser Glu Cys Asn Gly Trp Leu Ala Ala Glu Gly Arg Thr Asp Tyr 
        435                 440                 445             


Val Lys Leu Phe Leu Pro Ile Ala Ile Lys Met Arg Arg Asp Lys Asp 
    450                 455                 460                 


Val Ala Asn Thr Phe Ala Asp Ile Trp Gly Asp Phe His Glu Val Thr 
465                 470                 475                 480 


Gly Leu 
        


<210>  27
<211>  482
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Mutant ligase (Enterobacteria phage CC31 backbone) protein 
       sequence

<400>  27

Met Ile Leu Asp Ile Ile Asn Glu Ile Ala Ser Ile Gly Ser Thr Lys 
1               5                   10                  15      


Glu Lys Glu Ala Ile Ile Arg Arg His Lys Asp Asn Glu Leu Leu Lys 
            20                  25                  30          


Arg Val Phe Arg Met Thr Tyr Asp Gly Lys Leu Gln Tyr Tyr Ile Lys 
        35                  40                  45              


Lys Trp Asp Thr Arg Pro Lys Gly Asp Ile His Leu Thr Leu Glu Asp 
    50                  55                  60                  


Met Leu Tyr Leu Leu Glu Glu Lys Leu Ala Lys Arg Val Val Thr Gly 
65                  70                  75                  80  


Asn Ala Ala Lys Glu Lys Leu Glu Ile Ala Leu Ser Gln Thr Ser Asp 
                85                  90                  95      


Ala Asp Ala Glu Val Val Lys Lys Val Leu Leu Arg Asp Leu Arg Cys 
            100                 105                 110         


Gly Ala Ser Arg Ser Ile Ala Asn Lys Val Trp Lys Asn Leu Ile Pro 
        115                 120                 125             


Glu Gln Pro Gln Met Leu Ala Ser Ser Tyr Asp Glu Lys Gly Ile Glu 
    130                 135                 140                 


Lys Asn Ile Lys Phe Pro Ala Phe Ala Gln Leu Lys Ala Asp Gly Ala 
145                 150                 155                 160 


Arg Ala Phe Ala Glu Val Arg Gly Asp Glu Leu Asp Asp Val Lys Ile 
                165                 170                 175     


Leu Ser Arg Ala Gly Asn Glu Tyr Leu Gly Leu Asp Leu Leu Lys Gln 
            180                 185                 190         


Gln Leu Ile Glu Met Thr Lys Glu Ala Arg Glu Arg His Pro Gly Gly 
        195                 200                 205             


Val Met Ile Asp Gly Glu Leu Val Tyr His Ala Ser Thr Leu Pro Ala 
    210                 215                 220                 


Gly Pro Leu Asp Asp Ile Phe Gly Asp Leu Pro Glu Leu Ser Lys Ala 
225                 230                 235                 240 


Lys Glu Phe Lys Glu Glu Ser Arg Thr Met Ser Asn Gly Leu Ala Asn 
                245                 250                 255     


Lys Ser Leu Lys Gly Thr Ile Ser Ala Lys Glu Ala Ala Gly Met Lys 
            260                 265                 270         


Phe Gln Val Trp Asp Tyr Val Pro Leu Asp Val Val Tyr Ser Glu Gly 
        275                 280                 285             


Lys Gln Ser Gly Phe Ala Tyr Asp Val Arg Phe Arg Ala Leu Glu Leu 
    290                 295                 300                 


Met Val Gln Gly Tyr Ser Gln Met Ile Leu Ile Glu Asn His Ile Val 
305                 310                 315                 320 


His Asn Leu Asp Glu Ala Lys Val Ile Tyr Arg Lys Tyr Val Asp Glu 
                325                 330                 335     


Gly Leu Glu Gly Ile Ile Leu Lys Asn Ile Gly Ala Phe Trp Glu Asn 
            340                 345                 350         


Thr Arg Ser Lys Asn Leu Tyr Lys Phe Lys Arg Val Ile Phe Ile Asp 
        355                 360                 365             


Leu Arg Ile Val Asp Ile Tyr Glu His Ser Lys Gln Pro Gly Lys Ala 
    370                 375                 380                 


Gly Gly Phe Tyr Leu Glu Ser Glu Cys Gly Leu Ile Lys Val Lys Ala 
385                 390                 395                 400 


Gly Ser Gly Leu Lys Asp Lys Pro Gly Lys Asp Ala His Glu Leu Asp 
                405                 410                 415     


Arg Thr Arg Ile Trp Glu Asn Lys Asn Asp Tyr Ile Gly Gly Val Leu 
            420                 425                 430         


Glu Ser Glu Cys Asn Gly Trp Leu Ala Ala Glu Gly Arg Thr Asp Tyr 
        435                 440                 445             


Val Lys Leu Phe Leu Pro Ile Ala Ile Lys Met Arg Arg Asp Lys Asp 
    450                 455                 460                 


Val Ala Asn Thr Phe Ala Asp Ile Trp Gly Asp Phe His Glu Val Thr 
465                 470                 475                 480 


Gly Leu 
        


<210>  28
<211>  497
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Mutant ligase (Shigella phage Shf125875 backbone) protein 
       sequence

<400>  28

Met Ile Leu Asp Ile Leu Asn Gln Ile Ala Ala Ile Gly Ser Thr Lys 
1               5                   10                  15      


Thr Lys Gln Glu Ile Leu Lys Lys Asn Lys Asp Asn Lys Leu Leu Glu 
            20                  25                  30          


Arg Val Tyr Arg Leu Thr Tyr Ala Arg Gly Ile Gln Tyr Tyr Ile Lys 
        35                  40                  45              


Lys Trp Pro Gly Pro Gly Glu Arg Ser Gln Ala Tyr Gly Leu Leu Glu 
    50                  55                  60                  


Leu Asp Asp Met Leu Asp Phe Ile Glu Phe Thr Leu Ala Thr Arg Lys 
65                  70                  75                  80  


Leu Thr Gly Asn Ala Ala Ile Lys Glu Leu Met Gly Tyr Ile Ala Asp 
                85                  90                  95      


Gly Lys Pro Asp Asp Val Glu Val Leu Arg Arg Val Met Met Arg Asp 
            100                 105                 110         


Leu Glu Val Gly Ala Ser Val Ser Ile Ala Asn Lys Val Trp Pro Gly 
        115                 120                 125             


Leu Ile Gln Leu Gln Pro Gln Met Leu Ala Ser Ala Tyr Asp Glu Lys 
    130                 135                 140                 


Leu Ile Thr Lys Asn Ile Lys Trp Pro Ala Phe Ala Gln Leu Lys Ala 
145                 150                 155                 160 


Asp Gly Ala Arg Cys Phe Ala Glu Val Arg Asp Asp Gly Val Gln Phe 
                165                 170                 175     


Phe Ser Arg Ala Gly Asn Glu Tyr His Gly Leu Thr Leu Leu Ala Asp 
            180                 185                 190         


Glu Leu Met Glu Met Thr Lys Glu Ala Arg Glu Arg His Pro Asn Gly 
        195                 200                 205             


Val Leu Ile Asp Gly Glu Leu Val Tyr His Ser Phe Asp Ile Lys Lys 
    210                 215                 220                 


Ala Val Ser Ser Gly Asn Asp Leu Ser Phe Leu Phe Gly Asp Asn Glu 
225                 230                 235                 240 


Glu Ser Glu Glu Val Gln Val Ala Asp Arg Ser Thr Ser Asn Gly Leu 
                245                 250                 255     


Ala Asn Lys Ser Leu Gln Gly Thr Ile Ser Pro Lys Glu Ala Glu Gly 
            260                 265                 270         


Met Val Leu Gln Ala Trp Asp Tyr Val Pro Leu Asp Glu Val Tyr Ser 
        275                 280                 285             


Asp Gly Lys Ile Lys Gly Gln Lys Tyr Asp Val Arg Phe Ala Ala Leu 
    290                 295                 300                 


Glu Asn Met Ala Glu Gly Phe Lys Arg Ile Glu Pro Ile Glu Asn Gln 
305                 310                 315                 320 


Leu Val His Asn Leu Asp Glu Ala Lys Val Val Tyr Lys Lys Tyr Val 
                325                 330                 335     


Asp Gln Gly Leu Glu Gly Ile Ile Leu Lys Asn Arg Asp Ser Tyr Trp 
            340                 345                 350         


Glu Asn Lys Arg Ser Lys Asn Leu Ile Lys Phe Lys Arg Val Ile Val 
        355                 360                 365             


Ile Ala Leu Glu Val Val Gly Tyr Tyr Glu His Ser Lys Asp Pro Asn 
    370                 375                 380                 


Lys Leu Gly Gly Val Glu Leu Val Ser Arg Cys Arg Arg Ile Thr Thr 
385                 390                 395                 400 


Asp Cys Gly Ser Gly Phe Lys Asp Thr Thr His Lys Thr Val Asp Gly 
                405                 410                 415     


Val Lys Val Leu Ile Pro Leu Asp Glu Arg His Asp Leu Asp Arg Glu 
            420                 425                 430         


Arg Leu Met Ala Glu Ala Arg Glu Gly Lys Leu Ile Gly Arg Ile Ala 
        435                 440                 445             


Asp Cys Glu Cys Asn Gly Trp Val His Ser Lys Gly Arg Glu Gly Thr 
    450                 455                 460                 


Val Gly Ile Phe Leu Pro Ile Ile Lys Gly Phe Arg Phe Asp Lys Thr 
465                 470                 475                 480 


Glu Ala Asp Ser Phe Glu Asp Val Phe Gly Pro Trp Ser Gln Thr Gly 
                485                 490                 495     


Leu 
    


<210>  29
<211>  298
<212>  PRT
<213>  Paramecium bursaria Chlorella virus PBCV-1

<400>  29

Met Ala Ile Thr Lys Pro Leu Leu Ala Ala Thr Leu Glu Asn Ile Glu 
1               5                   10                  15      


Asp Val Gln Phe Pro Cys Leu Ala Thr Pro Lys Ile Asp Gly Ile Arg 
            20                  25                  30          


Ser Val Lys Gln Thr Gln Met Leu Ser Arg Thr Phe Lys Pro Ile Arg 
        35                  40                  45              


Asn Ser Val Met Asn Arg Leu Leu Thr Glu Leu Leu Pro Glu Gly Ser 
    50                  55                  60                  


Asp Gly Glu Ile Ser Ile Glu Gly Ala Thr Phe Gln Asp Thr Thr Ser 
65                  70                  75                  80  


Ala Val Met Thr Gly His Lys Met Tyr Asn Ala Lys Phe Ser Tyr Tyr 
                85                  90                  95      


Trp Phe Asp Tyr Val Thr Asp Asp Pro Leu Lys Lys Tyr Ile Asp Arg 
            100                 105                 110         


Val Glu Asp Met Lys Asn Tyr Ile Thr Val His Pro His Ile Leu Glu 
        115                 120                 125             


His Ala Gln Val Lys Ile Ile Pro Leu Ile Pro Val Glu Ile Asn Asn 
    130                 135                 140                 


Ile Thr Glu Leu Leu Gln Tyr Glu Arg Asp Val Leu Ser Lys Gly Phe 
145                 150                 155                 160 


Glu Gly Val Met Ile Arg Lys Pro Asp Gly Lys Tyr Lys Phe Gly Arg 
                165                 170                 175     


Ser Thr Leu Lys Glu Gly Ile Leu Leu Lys Met Lys Gln Phe Lys Asp 
            180                 185                 190         


Ala Glu Ala Thr Ile Ile Ser Met Thr Ala Leu Phe Lys Asn Thr Asn 
        195                 200                 205             


Thr Lys Thr Lys Asp Asn Phe Gly Tyr Ser Lys Arg Ser Thr His Lys 
    210                 215                 220                 


Ser Gly Lys Val Glu Glu Asp Val Met Gly Ser Ile Glu Val Asp Tyr 
225                 230                 235                 240 


Asp Gly Val Val Phe Ser Ile Gly Thr Gly Phe Asp Ala Asp Gln Arg 
                245                 250                 255     


Arg Asp Phe Trp Gln Asn Lys Glu Ser Tyr Ile Gly Lys Met Val Lys 
            260                 265                 270         


Phe Lys Tyr Phe Glu Met Gly Ser Lys Asp Cys Pro Arg Phe Pro Val 
        275                 280                 285             


Phe Ile Gly Ile Arg His Glu Glu Asp Arg 
    290                 295             


<210>  30
<211>  23
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Example 5, 8 and 9 template oligonucleotide sequence

<400>  30
tttggtgcga agcagactga ggc                                               23


<210>  31
<211>  23
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Example 5 template oligonucleotide sequence

<400>  31
tttggtgcga agcagagtga ggc                                               23


<210>  32
<211>  23
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Example 5 template oligonucleotide sequence

<400>  32
tttggtgcga agcagattga ggc                                               23


<210>  33
<211>  23
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Example 5 template oligonucleotide sequence

<400>  33
tttggtgcga agcagaatga ggc                                               23


<210>  34
<211>  23
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Example 5 template oligonucleotide sequence

<400>  34
tttggtgcga agcagtctga ggc                                               23


<210>  35
<211>  23
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Example 5 template oligonucleotide sequence

<400>  35
tttggtgcga agcagtgtga ggc                                               23


<210>  36
<211>  23
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Example 5 template oligonucleotide sequence

<400>  36
tttggtgcga agcagtttga ggc                                               23


<210>  37
<211>  23
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Example 5 template oligonucleotide sequence

<400>  37
tttggtgcga agcagtatga ggc                                               23


<210>  38
<211>  23
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Example 5 template oligonucleotide sequence

<400>  38
tttggtgcga agcagcctga ggc                                               23


<210>  39
<211>  23
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Example 5 template oligonucleotide sequence

<400>  39
tttggtgcga agcagcgtga ggc                                               23


<210>  40
<211>  23
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Example 5 template oligonucleotide sequence

<400>  40
tttggtgcga agcagcttga ggc                                               23


<210>  41
<211>  23
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Example 5 template oligonucleotide sequence

<400>  41
tttggtgcga agcagcatga ggc                                               23


<210>  42
<211>  23
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Example 5 template oligonucleotide sequence

<400>  42
tttggtgcga agcaggctga ggc                                               23


<210>  43
<211>  23
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Example 5 template oligonucleotide sequence

<400>  43
tttggtgcga agcagggtga ggc                                               23


<210>  44
<211>  23
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Example 5 template oligonucleotide sequence

<400>  44
tttggtgcga agcaggttga ggc                                               23


<210>  45
<211>  23
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Example 5 template oligonucleotide sequence

<400>  45
tttggtgcga agcaggatga ggc                                               23


<210>  46
<211>  20
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Example 14 "20 mer" oligonucleotide sequence

<400>  46
gccucagtct gcttcgcacc                                                   20


<210>  47
<211>  36
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Example 7 template oligonucleotide sequence

<400>  47
tttggtgcga agcagaaggt aagccgaggt ttggcc                                 36


<210>  48
<211>  298
<212>  PRT
<213>  Paramecium bursaria Chlorella virus NE-JV-4

<400>  48

Met Ala Ile Thr Lys Pro Leu Leu Ala Ala Thr Leu Glu Asn Ile Glu 
1               5                   10                  15      


Asp Val Gln Phe Pro Cys Leu Ala Thr Pro Lys Ile Asp Gly Ile Arg 
            20                  25                  30          


Ser Val Lys Gln Thr Gln Met Leu Ser Arg Thr Phe Lys Pro Ile Arg 
        35                  40                  45              


Asn Ser Val Met Asn Arg Leu Leu Thr Glu Leu Leu Pro Glu Gly Ser 
    50                  55                  60                  


Asp Gly Glu Ile Ser Ile Glu Gly Ala Thr Phe Gln Asp Thr Thr Ser 
65                  70                  75                  80  


Ala Val Met Thr Gly His Lys Met Tyr Asn Ala Lys Phe Ser Tyr Tyr 
                85                  90                  95      


Trp Phe Asp Tyr Val Thr Asp Asp Pro Leu Lys Lys Tyr Ser Asp Arg 
            100                 105                 110         


Val Glu Asp Met Lys Asn Tyr Ile Thr Ala His Pro His Ile Leu Asp 
        115                 120                 125             


His Glu Gln Val Lys Ile Ile Pro Leu Ile Pro Val Glu Ile Asn Asn 
    130                 135                 140                 


Ile Thr Glu Leu Leu Gln Tyr Glu Arg Asp Val Leu Ser Lys Gly Phe 
145                 150                 155                 160 


Glu Gly Val Met Ile Arg Lys Pro Asp Gly Lys Tyr Lys Phe Gly Arg 
                165                 170                 175     


Ser Thr Leu Lys Glu Gly Ile Leu Leu Lys Met Lys Gln Phe Lys Asp 
            180                 185                 190         


Ala Glu Ala Thr Ile Ile Ser Met Thr Ala Leu Phe Lys Asn Thr Asn 
        195                 200                 205             


Thr Lys Thr Lys Asp Asn Phe Gly Tyr Ser Lys Arg Ser Thr His Lys 
    210                 215                 220                 


Asn Gly Lys Val Glu Glu Asp Val Met Gly Ser Ile Glu Val Asp Tyr 
225                 230                 235                 240 


Asp Gly Val Val Phe Ser Ile Gly Thr Gly Phe Asp Ala Asp Gln Arg 
                245                 250                 255     


Arg Asp Phe Trp Gln Asn Lys Glu Ser Tyr Ile Gly Lys Met Val Lys 
            260                 265                 270         


Phe Lys Tyr Phe Glu Met Gly Ser Lys Asp Cys Pro Arg Phe Pro Val 
        275                 280                 285             


Phe Ile Gly Ile Arg His Glu Glu Asp His 
    290                 295             


<210>  49
<211>  298
<212>  PRT
<213>  Paramecium bursaria Chlorella virus NYs1

<400>  49

Met Thr Ile Ala Lys Pro Leu Leu Ala Ala Thr Leu Glu Asn Leu Asp 
1               5                   10                  15      


Asp Val Lys Phe Pro Cys Leu Val Thr Pro Lys Ile Asp Gly Ile Arg 
            20                  25                  30          


Ser Leu Lys Gln Gln His Met Leu Ser Arg Thr Phe Lys Pro Ile Arg 
        35                  40                  45              


Asn Ser Val Met Asn Lys Leu Leu Ser Glu Leu Leu Pro Glu Gly Ala 
    50                  55                  60                  


Asp Gly Glu Ile Cys Ile Glu Asp Ser Thr Phe Gln Ala Thr Thr Ser 
65                  70                  75                  80  


Ala Val Met Thr Gly His Lys Val Tyr Asp Glu Lys Phe Ser Tyr Tyr 
                85                  90                  95      


Trp Phe Asp Tyr Val Val Asp Asp Pro Leu Lys Ser Tyr Thr Asp Arg 
            100                 105                 110         


Val Asn Asp Met Lys Lys Tyr Val Asp Asp His Pro His Ile Leu Glu 
        115                 120                 125             


His Glu Gln Val Lys Ile Ile Pro Leu Ile Pro Val Glu Ile Asn Asn 
    130                 135                 140                 


Ile Asp Glu Leu Ser Gln Tyr Glu Arg Asp Val Leu Ala Lys Gly Phe 
145                 150                 155                 160 


Glu Gly Val Met Ile Arg Arg Pro Asp Gly Lys Tyr Lys Phe Gly Arg 
                165                 170                 175     


Ser Thr Leu Lys Glu Gly Ile Leu Leu Lys Met Lys Gln Phe Lys Asp 
            180                 185                 190         


Ala Glu Ala Thr Ile Ile Ser Met Ser Pro Arg Leu Lys Asn Thr Asn 
        195                 200                 205             


Ala Lys Ser Lys Asp Asn Leu Gly Tyr Ser Lys Arg Ser Thr His Lys 
    210                 215                 220                 


Ser Gly Lys Val Glu Glu Glu Thr Met Gly Ser Ile Glu Val Asp Tyr 
225                 230                 235                 240 


Asp Gly Val Val Phe Ser Ile Gly Thr Gly Phe Asp Asp Glu Gln Arg 
                245                 250                 255     


Lys His Phe Trp Glu Asn Lys Asp Ser Tyr Ile Gly Lys Leu Leu Lys 
            260                 265                 270         


Phe Lys Tyr Phe Glu Met Gly Ser Lys Asp Ala Pro Arg Phe Pro Val 
        275                 280                 285             


Phe Ile Gly Ile Arg His Glu Glu Asp Cys 
    290                 295             


<210>  50
<211>  301
<212>  PRT
<213>  Paramecium bursaria Chlorella virus NE-JV-1

<400>  50

Met Thr Ala Ile Gln Lys Pro Leu Leu Ala Ala Ser Phe Lys Lys Leu 
1               5                   10                  15      


Thr Val Ala Asp Val Lys Tyr Pro Val Phe Ala Thr Pro Lys Leu Asp 
            20                  25                  30          


Gly Ile Arg Ala Leu Lys Ile Asp Gly Ala Phe Val Ser Arg Thr Phe 
        35                  40                  45              


Lys Pro Ile Arg Asn Arg Ala Ile Ala Asp Ala Leu Gln Asp Leu Leu 
    50                  55                  60                  


Pro Asn Gly Ser Asp Gly Glu Ile Leu Ser Gly Ser Thr Phe Gln Asp 
65                  70                  75                  80  


Ala Ser Ser Ala Val Met Thr Ala Lys Ala Gly Ile Gly Ala Asn Thr 
                85                  90                  95      


Ile Phe Tyr Trp Phe Asp Tyr Val Lys Asp Asp Pro Asn Lys Pro Tyr 
            100                 105                 110         


Leu Asp Arg Met Thr Asp Met Glu Asn Tyr Leu Lys Glu Arg Pro Glu 
        115                 120                 125             


Ile Leu Asn Asp Asp Arg Ile Lys Ile Val Pro Leu Ile Pro Lys Lys 
    130                 135                 140                 


Ile Glu Thr Lys Asp Glu Leu Asp Thr Phe Glu Lys Ile Cys Leu Asp 
145                 150                 155                 160 


Gln Gly Phe Glu Gly Val Met Ile Arg Ser Gly Ala Gly Lys Tyr Lys 
                165                 170                 175     


Phe Gly Arg Ser Thr Glu Lys Glu Gly Ile Leu Ile Lys Ile Lys Gln 
            180                 185                 190         


Phe Glu Asp Asp Glu Ala Val Val Ile Gly Phe Thr Pro Met Gln Thr 
        195                 200                 205             


Asn Thr Asn Asp Lys Ser Met Asn Glu Leu Gly Asp Met Lys Arg Ser 
    210                 215                 220                 


Ser His Lys Asp Gly Lys Val Asn Leu Asp Thr Leu Gly Ala Leu Glu 
225                 230                 235                 240 


Val Asp Trp Asn Gly Ile Thr Phe Ser Ile Gly Thr Gly Phe Asp His 
                245                 250                 255     


Ala Leu Arg Asp Lys Leu Trp Ser Glu Arg Asp Lys Leu Ile Gly Lys 
            260                 265                 270         


Ile Val Lys Phe Lys Tyr Phe Ala Gln Gly Val Lys Thr Ala Pro Arg 
        275                 280                 285             


Phe Pro Val Phe Ile Gly Phe Arg Asp Pro Asp Asp Met 
    290                 295                 300     


<210>  51
<211>  300
<212>  PRT
<213>  Acanthocystis turfacea Chlorella virus Canal-1

<400>  51

Met Ala Ile Gln Lys Pro Leu Leu Ala Ala Ser Leu Lys Lys Met Ser 
1               5                   10                  15      


Val Gly Asp Leu Thr Phe Pro Val Phe Ala Thr Pro Lys Leu Asp Gly 
            20                  25                  30          


Ile Arg Ala Leu Lys Val Gly Gly Thr Ile Val Ser Arg Thr Phe Lys 
        35                  40                  45              


Pro Val Arg Asn Ser Ala Ile Ser Glu Val Leu Ala Ser Ile Leu Pro 
    50                  55                  60                  


Asp Gly Ser Asp Gly Glu Ile Leu Ser Gly Lys Thr Phe Gln Glu Ser 
65                  70                  75                  80  


Thr Ser Thr Val Met Thr Ala Asp Ala Gly Leu Gly Ser Gly Thr Met 
                85                  90                  95      


Phe Phe Trp Phe Asp Tyr Val Lys Asp Asp Pro Asn Lys Gly Tyr Leu 
            100                 105                 110         


Asp Arg Ile Ala Asp Met Lys Ser Phe Thr Asp Arg His Pro Glu Ile 
        115                 120                 125             


Leu Lys Asp Lys Arg Val Thr Ile Val Pro Leu Phe Pro Lys Lys Ile 
    130                 135                 140                 


Asp Thr Thr Glu Glu Leu His Glu Phe Glu Lys Trp Cys Leu Asp Gln 
145                 150                 155                 160 


Gly Phe Glu Gly Val Met Val Arg Asn Ala Gly Gly Lys Tyr Lys Phe 
                165                 170                 175     


Gly Arg Ser Thr Glu Lys Glu Gln Ile Leu Val Lys Ile Lys Gln Phe 
            180                 185                 190         


Glu Asp Asp Glu Ala Val Val Ile Gly Val Ser Ala Leu Gln Thr Asn 
        195                 200                 205             


Thr Asn Asp Lys Lys Leu Asn Gln Leu Gly Glu Met Arg Arg Thr Ser 
    210                 215                 220                 


His Gln Asp Gly Lys Val Glu Leu Glu Met Leu Gly Ala Leu Asp Val 
225                 230                 235                 240 


Asp Trp Asn Gly Ile Arg Phe Ser Ile Gly Thr Gly Phe Asp Arg Asp 
                245                 250                 255     


Thr Arg Val Asp Leu Trp Lys Arg Arg Glu Gly Val Ile Gly Lys Ile 
            260                 265                 270         


Val Lys Phe Lys Tyr Phe Ser Gln Gly Ile Lys Thr Ala Pro Arg Phe 
        275                 280                 285             


Pro Val Phe Leu Gly Phe Arg Asp Lys Asp Asp Met 
    290                 295                 300 


<210>  52
<211>  300
<212>  PRT
<213>  Acanthocystis turfacea Chlorella virus Br0604L

<400>  52

Met Ala Ile Gln Lys Pro Leu Leu Ala Ala Ser Leu Lys Lys Leu Ser 
1               5                   10                  15      


Val Asp Asp Leu Thr Phe Pro Val Tyr Ala Thr Pro Lys Leu Asp Gly 
            20                  25                  30          


Ile Arg Ala Leu Lys Ile Asp Gly Thr Leu Val Ser Arg Thr Phe Lys 
        35                  40                  45              


Pro Ile Arg Asn Thr Thr Ile Ser Lys Val Leu Thr Ser Leu Leu Pro 
    50                  55                  60                  


Asp Gly Ser Asp Gly Glu Ile Leu Ser Gly Lys Thr Phe Gln Asp Ser 
65                  70                  75                  80  


Thr Ser Thr Val Met Ser Ala Asp Ala Gly Ile Gly Ser Gly Thr Thr 
                85                  90                  95      


Phe Phe Trp Phe Asp Tyr Val Lys Asp Asp Pro Asn Lys Gly Tyr Leu 
            100                 105                 110         


Asp Arg Ile Ala Asp Ile Lys Lys Phe Ile Asp Cys Arg Pro Glu Ile 
        115                 120                 125             


Leu Lys Asp Ser Arg Val Ile Ile Val Pro Leu Phe Pro Lys Lys Ile 
    130                 135                 140                 


Asp Thr Ala Glu Glu Leu Asn Val Phe Glu Lys Trp Cys Leu Asp Gln 
145                 150                 155                 160 


Gly Phe Glu Gly Val Met Val Arg Asn Ala Gly Gly Lys Tyr Lys Phe 
                165                 170                 175     


Gly Arg Ser Thr Glu Lys Glu Gln Ile Leu Val Lys Ile Lys Gln Phe 
            180                 185                 190         


Glu Asp Asp Glu Ala Val Val Ile Gly Val Ser Ala Leu Gln Thr Asn 
        195                 200                 205             


Thr Asn Asp Lys Lys Val Asn Glu Leu Gly Glu Met Arg Arg Thr Ser 
    210                 215                 220                 


His Gln Asp Gly Lys Val Asp Leu Asp Met Leu Gly Ala Leu Asp Val 
225                 230                 235                 240 


Asp Trp Asn Gly Ile Arg Phe Gly Ile Gly Thr Gly Phe Asp Lys Asp 
                245                 250                 255     


Thr Arg Glu Asp Leu Trp Lys Arg Arg Asp Ser Ile Ile Gly Lys Ile 
            260                 265                 270         


Val Lys Phe Lys Tyr Phe Ser Gln Gly Val Lys Thr Ala Pro Arg Phe 
        275                 280                 285             


Pro Val Phe Leu Gly Phe Arg Asp Lys Asn Asp Met 
    290                 295                 300 


<210>  53
<211>  300
<212>  PRT
<213>  Acanthocystis turfacea Chlorella virus NE-JV-2

<400>  53

Met Ala Ile Gln Lys Pro Leu Leu Ala Ala Ser Leu Lys Lys Leu Ser 
1               5                   10                  15      


Val Asp Asp Leu Thr Phe Pro Val Tyr Ala Thr Pro Lys Leu Asp Gly 
            20                  25                  30          


Ile Arg Ala Leu Lys Ile Asp Gly Thr Ile Val Ser Arg Thr Phe Lys 
        35                  40                  45              


Pro Ile Arg Asn Thr Thr Ile Ser Asn Val Leu Met Ser Leu Leu Pro 
    50                  55                  60                  


Asp Gly Ser Asp Gly Glu Ile Leu Ser Gly Lys Thr Phe Gln Asp Ser 
65                  70                  75                  80  


Thr Ser Thr Val Met Ser Ala Asp Ala Gly Ile Gly Ser Gly Thr Thr 
                85                  90                  95      


Phe Phe Trp Phe Asp Tyr Val Lys Asp Asp Pro Asp Lys Gly Tyr Leu 
            100                 105                 110         


Asp Arg Ile Ala Asp Met Lys Lys Phe Val Asp Ser His Pro Glu Ile 
        115                 120                 125             


Leu Lys Asp Arg Arg Val Thr Ile Val Pro Leu Ile Pro Lys Lys Ile 
    130                 135                 140                 


Asp Thr Val Glu Glu Leu Asn Val Phe Glu Gln Trp Cys Leu Asp Gln 
145                 150                 155                 160 


Gly Phe Glu Gly Val Met Val Arg Asn Ala Gly Gly Lys Tyr Lys Phe 
                165                 170                 175     


Gly Arg Ser Thr Glu Lys Glu Gln Ile Leu Val Lys Ile Lys Gln Phe 
            180                 185                 190         


Glu Asp Asp Glu Ala Val Val Ile Gly Val Ser Ala Leu Gln Thr Asn 
        195                 200                 205             


Val Asn Asp Lys Lys Met Asn Glu Leu Gly Asp Met Arg Arg Thr Ser 
    210                 215                 220                 


His Lys Asp Gly Lys Ile Asp Leu Glu Met Leu Gly Ala Leu Asp Val 
225                 230                 235                 240 


Glu Trp Asn Gly Ile Arg Phe Gly Ile Gly Thr Gly Phe Asp Lys Asp 
                245                 250                 255     


Thr Arg Glu Asp Leu Trp Lys Lys Arg Asp Ser Ile Ile Gly Lys Val 
            260                 265                 270         


Val Lys Phe Lys Tyr Phe Ser Gln Gly Ile Lys Thr Ala Pro Arg Phe 
        275                 280                 285             


Pro Val Phe Leu Gly Phe Arg Asp Glu Asn Asp Met 
    290                 295                 300 


<210>  54
<211>  300
<212>  PRT
<213>  Acanthocystis turfacea Chlorella virus TN603.4.2

<400>  54

Met Ala Ile Gln Lys Pro Leu Leu Ala Ala Ser Leu Lys Lys Met Ser 
1               5                   10                  15      


Val Asp Asn Leu Thr Phe Pro Val Tyr Ala Thr Pro Lys Leu Asp Gly 
            20                  25                  30          


Ile Arg Ala Leu Lys Ile Asp Gly Thr Leu Val Ser Arg Thr Phe Lys 
        35                  40                  45              


Pro Ile Arg Asn Thr Thr Ile Ser Lys Val Leu Ala Ser Leu Leu Pro 
    50                  55                  60                  


Asp Gly Ser Asp Gly Glu Ile Leu Ser Gly Lys Thr Phe Gln Asp Ser 
65                  70                  75                  80  


Thr Ser Thr Val Met Thr Thr Asp Ala Gly Ile Gly Ser Asp Thr Thr 
                85                  90                  95      


Phe Phe Trp Phe Asp Tyr Val Lys Asp Asp Pro Asp Lys Gly Tyr Leu 
            100                 105                 110         


Asp Arg Ile Ala Asp Met Lys Thr Phe Val Asp Gln His Pro Glu Ile 
        115                 120                 125             


Leu Lys Asp Ser Cys Val Thr Ile Val Pro Leu Phe Pro Lys Lys Ile 
    130                 135                 140                 


Asp Thr Pro Glu Glu Leu His Val Phe Glu Lys Trp Cys Leu Asp Gln 
145                 150                 155                 160 


Gly Phe Glu Gly Val Met Val Arg Thr Ala Gly Gly Lys Tyr Lys Phe 
                165                 170                 175     


Gly Arg Ser Thr Glu Lys Glu Gln Ile Leu Val Lys Ile Lys Gln Phe 
            180                 185                 190         


Glu Asp Asp Glu Ala Val Val Ile Gly Val Ser Ala Leu Gln Thr Asn 
        195                 200                 205             


Thr Asn Asp Lys Lys Leu Asn Gln Leu Gly Glu Met Arg Arg Thr Ser 
    210                 215                 220                 


His Gln Asp Gly Lys Val Asp Leu Asp Met Leu Gly Ala Leu Asp Val 
225                 230                 235                 240 


Asp Trp Asn Gly Ile Arg Phe Ser Ile Gly Thr Gly Phe Asp Lys Asp 
                245                 250                 255     


Thr Arg Glu Asp Leu Trp Lys Gln Arg Asp Ser Ile Val Gly Lys Val 
            260                 265                 270         


Val Lys Phe Lys Tyr Phe Ser Gln Gly Ile Lys Thr Ala Pro Arg Phe 
        275                 280                 285             


Pro Val Phe Leu Gly Phe Arg Asp Glu Asn Asp Met 
    290                 295                 300 


<210>  55
<211>  300
<212>  PRT
<213>  Acanthocystis turfacea Chlorella virus GM0701.1

<400>  55

Met Ala Ile Gln Lys Pro Leu Leu Ala Ala Ser Leu Lys Lys Met Ser 
1               5                   10                  15      


Val Asp Asp Leu Thr Phe Pro Val Tyr Thr Thr Pro Lys Leu Asp Gly 
            20                  25                  30          


Ile Arg Ala Leu Lys Ile Asp Gly Thr Leu Val Ser Arg Thr Phe Lys 
        35                  40                  45              


Pro Val Arg Asn Ser Ala Ile Ser Glu Val Leu Ala Ser Leu Leu Pro 
    50                  55                  60                  


Asp Gly Ser Asp Gly Glu Ile Leu Ser Gly Lys Thr Phe Gln Asp Ser 
65                  70                  75                  80  


Thr Ser Thr Val Met Thr Thr Asp Ala Gly Ile Gly Ser Asp Thr Thr 
                85                  90                  95      


Phe Phe Trp Phe Asp Tyr Val Lys Asp Asp Pro Asn Lys Gly Tyr Leu 
            100                 105                 110         


Asp Arg Ile Ala Asp Met Lys Thr Phe Ile Asp Gln His Pro Glu Met 
        115                 120                 125             


Leu Lys Asp Asn His Val Thr Ile Val Pro Leu Ile Pro Lys Lys Ile 
    130                 135                 140                 


Asp Thr Val Glu Glu Leu Asn Ile Phe Glu Lys Trp Cys Leu Asp Gln 
145                 150                 155                 160 


Gly Phe Glu Gly Val Met Val Arg Asn Ala Gly Gly Lys Tyr Lys Phe 
                165                 170                 175     


Gly Arg Ser Thr Glu Lys Glu Gln Ile Leu Val Lys Ile Lys Gln Phe 
            180                 185                 190         


Glu Asp Asp Glu Ala Val Val Ile Gly Val Ser Ala Leu Gln Thr Asn 
        195                 200                 205             


Thr Asn Asp Lys Lys Leu Asn Gln Leu Gly Glu Met Arg Arg Thr Ser 
    210                 215                 220                 


His Gln Asp Gly Lys Ile Asp Leu Glu Met Leu Gly Ala Leu Asp Val 
225                 230                 235                 240 


Asp Trp Asn Gly Ile Arg Phe Ser Ile Gly Thr Gly Phe Asp Arg Asp 
                245                 250                 255     


Thr Arg Val Asp Leu Trp Lys Arg Arg Asp Gly Ile Val Gly Arg Thr 
            260                 265                 270         


Ile Lys Phe Lys Tyr Phe Gly Gln Gly Ile Lys Thr Ala Pro Arg Phe 
        275                 280                 285             


Pro Val Phe Leu Gly Phe Arg Asp Lys Asp Asp Met 
    290                 295                 300 


<210>  56
<211>  287
<212>  PRT
<213>  Synechococcus phage S-CRM01

<400>  56

Met Leu Ala Gly Asn Phe Asp Pro Lys Lys Ala Lys Phe Pro Tyr Cys 
1               5                   10                  15      


Ala Thr Pro Lys Ile Asp Gly Ile Arg Phe Leu Met Val Asn Gly Arg 
            20                  25                  30          


Ala Leu Ser Arg Thr Phe Lys Pro Ile Arg Asn Glu Tyr Ile Gln Lys 
        35                  40                  45              


Leu Leu Ser Lys His Leu Pro Asp Gly Ile Asp Gly Glu Leu Thr Cys 
    50                  55                  60                  


Gly Asp Thr Phe Gln Ser Ser Thr Ser Ala Ile Met Arg Ile Ala Gly 
65                  70                  75                  80  


Glu Pro Asp Phe Lys Ala Trp Ile Phe Asp Tyr Val Asp Pro Asp Ser 
                85                  90                  95      


Thr Ser Ile Leu Pro Phe Ile Glu Arg Phe Asp Gln Ile Ser Asp Ile 
            100                 105                 110         


Ile Tyr Asn Gly Pro Ile Pro Phe Lys His Gln Val Leu Gly Gln Ser 
        115                 120                 125             


Ile Leu Tyr Asn Ile Asp Asp Leu Asn Arg Tyr Glu Glu Ala Cys Leu 
    130                 135                 140                 


Asn Glu Gly Tyr Glu Gly Val Met Leu Arg Asp Pro Tyr Gly Thr Tyr 
145                 150                 155                 160 


Lys Phe Gly Arg Ser Ser Thr Asn Glu Gly Ile Leu Leu Lys Val Lys 
                165                 170                 175     


Arg Phe Glu Asp Ala Glu Ala Thr Val Ile Arg Ile Asp Glu Lys Met 
            180                 185                 190         


Ser Asn Gln Asn Ile Ala Glu Lys Asp Asn Phe Gly Arg Thr Lys Arg 
        195                 200                 205             


Ser Ser Cys Leu Asp Gly Met Val Pro Met Glu Thr Thr Gly Ala Leu 
    210                 215                 220                 


Phe Val Arg Asn Ser Asp Gly Leu Glu Phe Ser Ile Gly Ser Gly Leu 
225                 230                 235                 240 


Asn Asp Glu Met Arg Asp Glu Ile Trp Lys Asn Lys Ser Ser Tyr Ile 
                245                 250                 255     


Gly Lys Leu Val Lys Tyr Lys Tyr Phe Pro Gln Gly Val Lys Asp Leu 
            260                 265                 270         


Pro Arg His Pro Val Phe Leu Gly Phe Arg Asp Pro Asp Asp Met 
        275                 280                 285         


<210>  57
<211>  322
<212>  PRT
<213>  marine sediment metagenome

<400>  57

Met Asp Ala His Glu Leu Met Lys Leu Asn Glu Tyr Ala Glu Arg Gln 
1               5                   10                  15      


Asn Gln Lys Gln Lys Lys Gln Ile Thr Lys Pro Met Leu Ala Ala Ser 
            20                  25                  30          


Leu Lys Asp Ile Thr Gln Leu Asp Tyr Ser Lys Gly Tyr Leu Ala Thr 
        35                  40                  45              


Gln Lys Leu Asp Gly Ile Arg Ala Leu Met Ile Asp Gly Lys Leu Val 
    50                  55                  60                  


Ser Arg Thr Phe Lys Pro Ile Arg Asn Asn His Ile Arg Glu Met Leu 
65                  70                  75                  80  


Glu Asp Val Leu Pro Asp Gly Ala Asp Gly Glu Ile Val Cys Pro Gly 
                85                  90                  95      


Ala Phe Gln Ala Thr Ser Ser Gly Val Met Ser Ala Asn Gly Glu Pro 
            100                 105                 110         


Glu Phe Ile Tyr Tyr Met Phe Asp Tyr Val Lys Asp Asp Ile Thr Lys 
        115                 120                 125             


Glu Tyr Trp Arg Arg Thr Gln Asp Met Val Gln Trp Leu Ile Asn Gln 
    130                 135                 140                 


Gly Pro Thr Arg Thr Pro Gly Leu Ser Lys Leu Lys Leu Leu Val Pro 
145                 150                 155                 160 


Thr Leu Ile Lys Asn Tyr Asp His Leu Lys Thr Tyr Glu Thr Glu Cys 
                165                 170                 175     


Ile Asp Lys Gly Phe Glu Gly Val Ile Leu Arg Thr Pro Asp Ser Pro 
            180                 185                 190         


Tyr Lys Cys Gly Arg Ser Thr Ala Lys Gln Glu Trp Leu Leu Lys Leu 
        195                 200                 205             


Lys Arg Phe Ala Asp Asp Glu Ala Val Val Ile Gly Phe Thr Glu Lys 
    210                 215                 220                 


Met His Asn Asp Asn Glu Ala Thr Lys Asp Lys Phe Gly His Thr Val 
225                 230                 235                 240 


Arg Ser Ser His Lys Glu Asn Lys Arg Pro Ala Gly Thr Leu Gly Ser 
                245                 250                 255     


Leu Ile Val Arg Asp Ile Lys Thr Glu Ile Glu Phe Glu Ile Gly Thr 
            260                 265                 270         


Gly Phe Asp Asp Glu Leu Arg Gln Lys Ile Trp Asp Ala Arg Pro Glu 
        275                 280                 285             


Trp Asp Gly Leu Cys Val Lys Tyr Lys His Phe Ala Ile Ser Gly Val 
    290                 295                 300                 


Lys Glu Lys Pro Arg Phe Pro Ser Phe Ile Gly Val Arg Asp Val Glu 
305                 310                 315                 320 


Asp Met 
        


<210>  58
<211>  691
<212>  PRT
<213>  Mycobacterium tuberculosis (strain ATCC 25618/H37Rv)

<400>  58

Met Ser Ser Pro Asp Ala Asp Gln Thr Ala Pro Glu Val Leu Arg Gln 
1               5                   10                  15      


Trp Gln Ala Leu Ala Glu Glu Val Arg Glu His Gln Phe Arg Tyr Tyr 
            20                  25                  30          


Val Arg Asp Ala Pro Ile Ile Ser Asp Ala Glu Phe Asp Glu Leu Leu 
        35                  40                  45              


Arg Arg Leu Glu Ala Leu Glu Glu Gln His Pro Glu Leu Arg Thr Pro 
    50                  55                  60                  


Asp Ser Pro Thr Gln Leu Val Gly Gly Ala Gly Phe Ala Thr Asp Phe 
65                  70                  75                  80  


Glu Pro Val Asp His Leu Glu Arg Met Leu Ser Leu Asp Asn Ala Phe 
                85                  90                  95      


Thr Ala Asp Glu Leu Ala Ala Trp Ala Gly Arg Ile His Ala Glu Val 
            100                 105                 110         


Gly Asp Ala Ala His Tyr Leu Cys Glu Leu Lys Ile Asp Gly Val Ala 
        115                 120                 125             


Leu Ser Leu Val Tyr Arg Glu Gly Arg Leu Thr Arg Ala Ser Thr Arg 
    130                 135                 140                 


Gly Asp Gly Arg Thr Gly Glu Asp Val Thr Leu Asn Ala Arg Thr Ile 
145                 150                 155                 160 


Ala Asp Val Pro Glu Arg Leu Thr Pro Gly Asp Asp Tyr Pro Val Pro 
                165                 170                 175     


Glu Val Leu Glu Val Arg Gly Glu Val Phe Phe Arg Leu Asp Asp Phe 
            180                 185                 190         


Gln Ala Leu Asn Ala Ser Leu Val Glu Glu Gly Lys Ala Pro Phe Ala 
        195                 200                 205             


Asn Pro Arg Asn Ser Ala Ala Gly Ser Leu Arg Gln Lys Asp Pro Ala 
    210                 215                 220                 


Val Thr Ala Arg Arg Arg Leu Arg Met Ile Cys His Gly Leu Gly His 
225                 230                 235                 240 


Val Glu Gly Phe Arg Pro Ala Thr Leu His Gln Ala Tyr Leu Ala Leu 
                245                 250                 255     


Arg Ala Trp Gly Leu Pro Val Ser Glu His Thr Thr Leu Ala Thr Asp 
            260                 265                 270         


Leu Ala Gly Val Arg Glu Arg Ile Asp Tyr Trp Gly Glu His Arg His 
        275                 280                 285             


Glu Val Asp His Glu Ile Asp Gly Val Val Val Lys Val Asp Glu Val 
    290                 295                 300                 


Ala Leu Gln Arg Arg Leu Gly Ser Thr Ser Arg Ala Pro Arg Trp Ala 
305                 310                 315                 320 


Ile Ala Tyr Lys Tyr Pro Pro Glu Glu Ala Gln Thr Lys Leu Leu Asp 
                325                 330                 335     


Ile Arg Val Asn Val Gly Arg Thr Gly Arg Ile Thr Pro Phe Ala Phe 
            340                 345                 350         


Met Thr Pro Val Lys Val Ala Gly Ser Thr Val Gly Gln Ala Thr Leu 
        355                 360                 365             


His Asn Ala Ser Glu Ile Lys Arg Lys Gly Val Leu Ile Gly Asp Thr 
    370                 375                 380                 


Val Val Ile Arg Lys Ala Gly Asp Val Ile Pro Glu Val Leu Gly Pro 
385                 390                 395                 400 


Val Val Glu Leu Arg Asp Gly Ser Glu Arg Glu Phe Ile Met Pro Thr 
                405                 410                 415     


Thr Cys Pro Glu Cys Gly Ser Pro Leu Ala Pro Glu Lys Glu Gly Asp 
            420                 425                 430         


Ala Asp Ile Arg Cys Pro Asn Ala Arg Gly Cys Pro Gly Gln Leu Arg 
        435                 440                 445             


Glu Arg Val Phe His Val Ala Ser Arg Asn Gly Leu Asp Ile Glu Val 
    450                 455                 460                 


Leu Gly Tyr Glu Ala Gly Val Ala Leu Leu Gln Ala Lys Val Ile Ala 
465                 470                 475                 480 


Asp Glu Gly Glu Leu Phe Ala Leu Thr Glu Arg Asp Leu Leu Arg Thr 
                485                 490                 495     


Asp Leu Phe Arg Thr Lys Ala Gly Glu Leu Ser Ala Asn Gly Lys Arg 
            500                 505                 510         


Leu Leu Val Asn Leu Asp Lys Ala Lys Ala Ala Pro Leu Trp Arg Val 
        515                 520                 525             


Leu Val Ala Leu Ser Ile Arg His Val Gly Pro Thr Ala Ala Arg Ala 
    530                 535                 540                 


Leu Ala Thr Glu Phe Gly Ser Leu Asp Ala Ile Ala Ala Ala Ser Thr 
545                 550                 555                 560 


Asp Gln Leu Ala Ala Val Glu Gly Val Gly Pro Thr Ile Ala Ala Ala 
                565                 570                 575     


Val Thr Glu Trp Phe Ala Val Asp Trp His Arg Glu Ile Val Asp Lys 
            580                 585                 590         


Trp Arg Ala Ala Gly Val Arg Met Val Asp Glu Arg Asp Glu Ser Val 
        595                 600                 605             


Pro Arg Thr Leu Ala Gly Leu Thr Ile Val Val Thr Gly Ser Leu Thr 
    610                 615                 620                 


Gly Phe Ser Arg Asp Asp Ala Lys Glu Ala Ile Val Ala Arg Gly Gly 
625                 630                 635                 640 


Lys Ala Ala Gly Ser Val Ser Lys Lys Thr Asn Tyr Val Val Ala Gly 
                645                 650                 655     


Asp Ser Pro Gly Ser Lys Tyr Asp Lys Ala Val Glu Leu Gly Val Pro 
            660                 665                 670         


Ile Leu Asp Glu Asp Gly Phe Arg Arg Leu Leu Ala Asp Gly Pro Ala 
        675                 680                 685             


Ser Arg Thr 
    690     


<210>  59
<211>  676
<212>  PRT
<213>  Enterococcus faecalis (strain ATCC 700802/V583)

<400>  59

Met Glu Gln Gln Pro Leu Thr Leu Thr Ala Ala Thr Thr Arg Ala Gln 
1               5                   10                  15      


Glu Leu Arg Lys Gln Leu Asn Gln Tyr Ser His Glu Tyr Tyr Val Lys 
            20                  25                  30          


Asp Gln Pro Ser Val Glu Asp Tyr Val Tyr Asp Arg Leu Tyr Lys Glu 
        35                  40                  45              


Leu Val Asp Ile Glu Thr Glu Phe Pro Asp Leu Ile Thr Pro Asp Ser 
    50                  55                  60                  


Pro Thr Gln Arg Val Gly Gly Lys Val Leu Ser Gly Phe Glu Lys Ala 
65                  70                  75                  80  


Pro His Asp Ile Pro Met Tyr Ser Leu Asn Asp Gly Phe Ser Lys Glu 
                85                  90                  95      


Asp Ile Phe Ala Phe Asp Glu Arg Val Arg Lys Ala Ile Gly Lys Pro 
            100                 105                 110         


Val Ala Tyr Cys Cys Glu Leu Lys Ile Asp Gly Leu Ala Ile Ser Leu 
        115                 120                 125             


Arg Tyr Glu Asn Gly Val Phe Val Arg Gly Ala Thr Arg Gly Asp Gly 
    130                 135                 140                 


Thr Val Gly Glu Asn Ile Thr Glu Asn Leu Arg Thr Val Arg Ser Val 
145                 150                 155                 160 


Pro Met Arg Leu Thr Glu Pro Ile Ser Val Glu Val Arg Gly Glu Cys 
                165                 170                 175     


Tyr Met Pro Lys Gln Ser Phe Val Ala Leu Asn Glu Glu Arg Glu Glu 
            180                 185                 190         


Asn Gly Gln Asp Ile Phe Ala Asn Pro Arg Asn Ala Ala Ala Gly Ser 
        195                 200                 205             


Leu Arg Gln Leu Asp Thr Lys Ile Val Ala Lys Arg Asn Leu Asn Thr 
    210                 215                 220                 


Phe Leu Tyr Thr Val Ala Asp Phe Gly Pro Met Lys Ala Lys Thr Gln 
225                 230                 235                 240 


Phe Glu Ala Leu Glu Glu Leu Ser Ala Ile Gly Phe Arg Thr Asn Pro 
                245                 250                 255     


Glu Arg Gln Leu Cys Gln Ser Ile Asp Glu Val Trp Ala Tyr Ile Glu 
            260                 265                 270         


Glu Tyr His Glu Lys Arg Ser Thr Leu Pro Tyr Glu Ile Asp Gly Ile 
        275                 280                 285             


Val Ile Lys Val Asn Glu Phe Ala Leu Gln Asp Glu Leu Gly Phe Thr 
    290                 295                 300                 


Val Lys Ala Pro Arg Trp Ala Ile Ala Tyr Lys Phe Pro Pro Glu Glu 
305                 310                 315                 320 


Ala Glu Thr Val Val Glu Asp Ile Glu Trp Thr Ile Gly Arg Thr Gly 
                325                 330                 335     


Val Val Thr Pro Thr Ala Val Met Ala Pro Val Arg Val Ala Gly Thr 
            340                 345                 350         


Thr Val Ser Arg Ala Ser Leu His Asn Ala Asp Phe Ile Gln Met Lys 
        355                 360                 365             


Asp Ile Arg Leu Asn Asp His Val Ile Ile Tyr Lys Ala Gly Asp Ile 
    370                 375                 380                 


Ile Pro Glu Val Ala Gln Val Leu Val Glu Lys Arg Ala Ala Asp Ser 
385                 390                 395                 400 


Gln Pro Tyr Glu Met Pro Thr His Cys Pro Ile Cys His Ser Glu Leu 
                405                 410                 415     


Val His Leu Asp Glu Glu Val Ala Leu Arg Cys Ile Asn Pro Lys Cys 
            420                 425                 430         


Pro Ala Gln Ile Lys Glu Gly Leu Asn His Phe Val Ser Arg Asn Ala 
        435                 440                 445             


Met Asn Ile Asp Gly Leu Gly Pro Arg Val Leu Ala Gln Met Tyr Asp 
    450                 455                 460                 


Lys Gly Leu Val Lys Asp Val Ala Asp Leu Tyr Phe Leu Thr Glu Glu 
465                 470                 475                 480 


Gln Leu Met Thr Leu Asp Lys Ile Lys Glu Lys Ser Ala Asn Asn Ile 
                485                 490                 495     


Tyr Thr Ala Ile Gln Gly Ser Lys Glu Asn Ser Val Glu Arg Leu Ile 
            500                 505                 510         


Phe Gly Leu Gly Ile Arg His Val Gly Ala Lys Ala Ala Lys Ile Leu 
        515                 520                 525             


Ala Glu His Phe Gly Asp Leu Pro Thr Leu Ser Arg Ala Thr Ala Glu 
    530                 535                 540                 


Glu Ile Val Ala Leu Asp Ser Ile Gly Glu Thr Ile Ala Asp Ser Val 
545                 550                 555                 560 


Val Thr Tyr Phe Glu Asn Glu Glu Val His Glu Leu Met Ala Glu Leu 
                565                 570                 575     


Glu Lys Ala Gln Val Asn Leu Thr Tyr Lys Gly Leu Arg Thr Glu Gln 
            580                 585                 590         


Leu Ala Glu Val Glu Ser Pro Phe Lys Asp Lys Thr Val Val Leu Thr 
        595                 600                 605             


Gly Lys Leu Ala Gln Tyr Thr Arg Glu Glu Ala Lys Glu Lys Ile Glu 
    610                 615                 620                 


Asn Leu Gly Gly Lys Val Thr Gly Ser Val Ser Lys Lys Thr Asp Ile 
625                 630                 635                 640 


Val Val Ala Gly Glu Asp Ala Gly Ser Lys Leu Thr Lys Ala Glu Ser 
                645                 650                 655     


Leu Gly Val Thr Val Trp Asn Glu Gln Glu Met Val Asp Ala Leu Asp 
            660                 665                 670         


Ala Ser His Phe 
        675     


<210>  60
<211>  670
<212>  PRT
<213>  Haemophilus influenzae (strain ATCC 51907/DSM 11121/ KW20/Rd)

<400>  60

Met Thr Asn Ile Gln Thr Gln Leu Asp Asn Leu Arg Lys Thr Leu Arg 
1               5                   10                  15      


Gln Tyr Glu Tyr Glu Tyr His Val Leu Asp Asn Pro Ser Val Pro Asp 
            20                  25                  30          


Ser Glu Tyr Asp Arg Leu Phe His Gln Leu Lys Ala Leu Glu Leu Glu 
        35                  40                  45              


His Pro Glu Phe Leu Thr Ser Asp Ser Pro Thr Gln Arg Val Gly Ala 
    50                  55                  60                  


Lys Pro Leu Ser Gly Phe Ser Gln Ile Arg His Glu Ile Pro Met Leu 
65                  70                  75                  80  


Ser Leu Asp Asn Ala Phe Ser Asp Ala Glu Phe Asn Ala Phe Val Lys 
                85                  90                  95      


Arg Ile Glu Asp Arg Leu Ile Leu Leu Pro Lys Pro Leu Thr Phe Cys 
            100                 105                 110         


Cys Glu Pro Lys Leu Asp Gly Leu Ala Val Ser Ile Leu Tyr Val Asn 
        115                 120                 125             


Gly Glu Leu Thr Gln Ala Ala Thr Arg Gly Asp Gly Thr Thr Gly Glu 
    130                 135                 140                 


Asp Ile Thr Ala Asn Ile Arg Thr Ile Arg Asn Val Pro Leu Gln Leu 
145                 150                 155                 160 


Leu Thr Asp Asn Pro Pro Ala Arg Leu Glu Val Arg Gly Glu Val Phe 
                165                 170                 175     


Met Pro His Ala Gly Phe Glu Arg Leu Asn Lys Tyr Ala Leu Glu His 
            180                 185                 190         


Asn Glu Lys Thr Phe Ala Asn Pro Arg Asn Ala Ala Ala Gly Ser Leu 
        195                 200                 205             


Arg Gln Leu Asp Pro Asn Ile Thr Ser Lys Arg Pro Leu Val Leu Asn 
    210                 215                 220                 


Ala Tyr Gly Ile Gly Ile Ala Glu Gly Val Asp Leu Pro Thr Thr His 
225                 230                 235                 240 


Tyr Ala Arg Leu Gln Trp Leu Lys Ser Ile Gly Ile Pro Val Asn Pro 
                245                 250                 255     


Glu Ile Arg Leu Cys Asn Gly Ala Asp Glu Val Leu Gly Phe Tyr Arg 
            260                 265                 270         


Asp Ile Gln Asn Lys Arg Ser Ser Leu Gly Tyr Asp Ile Asp Gly Thr 
        275                 280                 285             


Val Leu Lys Ile Asn Asp Ile Ala Leu Gln Asn Glu Leu Gly Phe Ile 
    290                 295                 300                 


Ser Lys Ala Pro Arg Trp Ala Ile Ala Tyr Lys Phe Pro Ala Gln Glu 
305                 310                 315                 320 


Glu Leu Thr Leu Leu Asn Asp Val Glu Phe Gln Val Gly Arg Thr Gly 
                325                 330                 335     


Ala Ile Thr Pro Val Ala Lys Leu Glu Pro Val Phe Val Ala Gly Val 
            340                 345                 350         


Thr Val Ser Asn Ala Thr Leu His Asn Gly Asp Glu Ile Glu Arg Leu 
        355                 360                 365             


Asn Ile Ala Ile Gly Asp Thr Val Val Ile Arg Arg Ala Gly Asp Val 
    370                 375                 380                 


Ile Pro Gln Ile Ile Gly Val Leu His Glu Arg Arg Pro Asp Asn Ala 
385                 390                 395                 400 


Lys Pro Ile Ile Phe Pro Thr Asn Cys Pro Val Cys Asp Ser Gln Ile 
                405                 410                 415     


Ile Arg Ile Glu Gly Glu Ala Val Ala Arg Cys Thr Gly Gly Leu Phe 
            420                 425                 430         


Cys Ala Ala Gln Arg Lys Glu Ala Leu Lys His Phe Val Ser Arg Lys 
        435                 440                 445             


Ala Met Asp Ile Asp Gly Val Gly Gly Lys Leu Ile Glu Gln Leu Val 
    450                 455                 460                 


Asp Arg Glu Leu Ile His Thr Pro Ala Asp Leu Phe Lys Leu Asp Leu 
465                 470                 475                 480 


Thr Thr Leu Thr Arg Leu Glu Arg Met Gly Ala Lys Ser Ala Glu Asn 
                485                 490                 495     


Ala Leu Asn Ser Leu Glu Asn Ala Lys Ser Thr Thr Leu Ala Arg Phe 
            500                 505                 510         


Ile Phe Ala Leu Gly Ile Arg Glu Val Gly Glu Ala Thr Ala Leu Asn 
        515                 520                 525             


Leu Ala Asn His Phe Lys Thr Leu Asp Ala Leu Lys Asp Ala Asn Leu 
    530                 535                 540                 


Glu Glu Leu Gln Gln Val Pro Asp Val Gly Glu Val Val Ala Asn Arg 
545                 550                 555                 560 


Ile Phe Ile Phe Trp Arg Glu Ala His Asn Val Ala Val Val Glu Asp 
                565                 570                 575     


Leu Ile Ala Gln Gly Val His Trp Glu Thr Val Glu Val Lys Glu Ala 
            580                 585                 590         


Ser Glu Asn Leu Phe Lys Asp Lys Thr Val Val Leu Thr Gly Thr Leu 
        595                 600                 605             


Thr Gln Met Gly Arg Asn Glu Ala Lys Ala Leu Leu Gln Gln Leu Gly 
    610                 615                 620                 


Ala Lys Val Ser Gly Ser Val Ser Ser Lys Thr Asp Phe Val Ile Ala 
625                 630                 635                 640 


Gly Asp Ala Ala Gly Ser Lys Leu Ala Lys Ala Gln Glu Leu Asn Ile 
                645                 650                 655     


Thr Val Leu Thr Glu Glu Glu Phe Leu Ala Gln Ile Thr Arg 
            660                 665                 670 


<210>  61
<211>  667
<212>  PRT
<213>  Staphylococcus aureus

<400>  61

Met Ala Asp Leu Ser Ser Arg Val Asn Glu Leu His Asp Leu Leu Asn 
1               5                   10                  15      


Gln Tyr Ser Tyr Glu Tyr Tyr Val Glu Asp Asn Pro Ser Val Pro Asp 
            20                  25                  30          


Ser Glu Tyr Asp Lys Leu Leu His Glu Leu Ile Lys Ile Glu Glu Glu 
        35                  40                  45              


His Pro Glu Tyr Lys Thr Val Asp Ser Pro Thr Val Arg Val Gly Gly 
    50                  55                  60                  


Glu Ala Gln Ala Ser Phe Asn Lys Val Asn His Asp Thr Pro Met Leu 
65                  70                  75                  80  


Ser Leu Gly Asn Ala Phe Asn Glu Asp Asp Leu Arg Lys Phe Asp Gln 
                85                  90                  95      


Arg Ile Arg Glu Gln Ile Gly Asn Val Glu Tyr Met Cys Glu Leu Lys 
            100                 105                 110         


Ile Asp Gly Leu Ala Val Ser Leu Lys Tyr Val Asp Gly Tyr Phe Val 
        115                 120                 125             


Gln Gly Leu Thr Arg Gly Asp Gly Thr Thr Gly Glu Asp Ile Thr Glu 
    130                 135                 140                 


Asn Leu Lys Thr Ile His Ala Ile Pro Leu Lys Met Lys Glu Pro Leu 
145                 150                 155                 160 


Asn Val Glu Val Arg Gly Glu Ala Tyr Met Pro Arg Arg Ser Phe Leu 
                165                 170                 175     


Arg Leu Asn Glu Glu Lys Glu Lys Asn Asp Glu Gln Leu Phe Ala Asn 
            180                 185                 190         


Pro Arg Asn Ala Ala Ala Gly Ser Leu Arg Gln Leu Asp Ser Lys Leu 
        195                 200                 205             


Thr Ala Lys Arg Lys Leu Ser Val Phe Ile Tyr Ser Val Asn Asp Phe 
    210                 215                 220                 


Thr Asp Phe Asn Ala Arg Ser Gln Ser Glu Ala Leu Asp Glu Leu Asp 
225                 230                 235                 240 


Lys Leu Gly Phe Thr Thr Asn Lys Asn Arg Ala Arg Val Asn Asn Ile 
                245                 250                 255     


Asp Gly Val Leu Glu Tyr Ile Glu Lys Trp Thr Ser Gln Arg Glu Ser 
            260                 265                 270         


Leu Pro Tyr Asp Ile Asp Gly Ile Val Ile Lys Val Asn Asp Leu Asp 
        275                 280                 285             


Gln Gln Asp Glu Met Gly Phe Thr Gln Lys Ser Pro Arg Trp Ala Ile 
    290                 295                 300                 


Ala Tyr Lys Phe Pro Ala Glu Glu Val Val Thr Lys Leu Leu Asp Ile 
305                 310                 315                 320 


Glu Leu Ser Ile Gly Arg Thr Gly Val Val Thr Pro Thr Ala Ile Leu 
                325                 330                 335     


Glu Pro Val Lys Val Ala Gly Thr Thr Val Ser Arg Ala Ser Leu His 
            340                 345                 350         


Asn Glu Asp Leu Ile His Asp Arg Asp Ile Arg Ile Gly Asp Ser Val 
        355                 360                 365             


Val Val Lys Lys Ala Gly Asp Ile Ile Pro Glu Val Val Arg Ser Ile 
    370                 375                 380                 


Pro Glu Arg Arg Pro Glu Asp Ala Val Thr Tyr His Met Pro Thr His 
385                 390                 395                 400 


Cys Pro Ser Cys Gly His Glu Leu Val Arg Ile Glu Gly Glu Val Ala 
                405                 410                 415     


Leu Arg Cys Ile Asn Pro Lys Cys Gln Ala Gln Leu Val Glu Gly Leu 
            420                 425                 430         


Ile His Phe Val Ser Arg Gln Ala Met Asn Ile Asp Gly Leu Gly Thr 
        435                 440                 445             


Lys Ile Ile Gln Gln Leu Tyr Gln Ser Glu Leu Ile Lys Asp Val Ala 
    450                 455                 460                 


Asp Ile Phe Tyr Leu Thr Glu Glu Asp Leu Leu Pro Leu Asp Arg Met 
465                 470                 475                 480 


Gly Gln Lys Lys Val Asp Asn Leu Leu Ala Ala Ile Gln Gln Ala Lys 
                485                 490                 495     


Asp Asn Ser Leu Glu Asn Leu Leu Phe Gly Leu Gly Ile Arg His Leu 
            500                 505                 510         


Gly Val Lys Ala Ser Gln Val Leu Ala Glu Lys Tyr Glu Thr Ile Asp 
        515                 520                 525             


Arg Leu Leu Thr Val Thr Glu Ala Glu Leu Val Glu Ile His Asp Ile 
    530                 535                 540                 


Gly Asp Lys Val Ala Gln Ser Val Val Thr Tyr Leu Glu Asn Glu Asp 
545                 550                 555                 560 


Ile Arg Ala Leu Ile Gln Lys Leu Lys Asp Lys His Val Asn Met Ile 
                565                 570                 575     


Tyr Lys Gly Ile Lys Thr Ser Asp Ile Glu Gly His Pro Glu Phe Ser 
            580                 585                 590         


Gly Lys Thr Ile Val Leu Thr Gly Lys Leu His Gln Met Thr Arg Asn 
        595                 600                 605             


Glu Ala Ser Lys Trp Leu Ala Ser Gln Gly Ala Lys Val Thr Ser Ser 
    610                 615                 620                 


Val Thr Lys Asn Thr Asp Val Val Ile Ala Gly Glu Asp Ala Gly Ser 
625                 630                 635                 640 


Lys Leu Thr Lys Ala Gln Ser Leu Gly Ile Glu Ile Trp Thr Glu Gln 
                645                 650                 655     


Gln Phe Val Asp Lys Gln Asn Glu Leu Asn Ser 
            660                 665         


<210>  62
<211>  652
<212>  PRT
<213>  Streptococcus pneumoniae (strain P1031)

<400>  62

Met Asn Lys Arg Met Asn Glu Leu Val Ala Leu Leu Asn Arg Tyr Ala 
1               5                   10                  15      


Thr Glu Tyr Tyr Thr Ser Asp Asn Pro Ser Val Ser Asp Ser Glu Tyr 
            20                  25                  30          


Asp Arg Leu Tyr Arg Glu Leu Val Glu Leu Glu Thr Ala Tyr Pro Glu 
        35                  40                  45              


Gln Val Leu Ala Asp Ser Pro Thr His Arg Val Gly Gly Lys Val Leu 
    50                  55                  60                  


Asp Gly Phe Glu Lys Tyr Ser His Gln Tyr Pro Leu Tyr Ser Leu Gln 
65                  70                  75                  80  


Asp Ala Phe Ser Arg Glu Glu Leu Asp Ala Phe Asp Ala Arg Val Arg 
                85                  90                  95      


Lys Glu Val Ala His Pro Thr Tyr Ile Cys Glu Leu Lys Ile Asp Gly 
            100                 105                 110         


Leu Ser Ile Ser Leu Thr Tyr Glu Lys Gly Ile Leu Val Ala Gly Val 
        115                 120                 125             


Thr Arg Gly Asp Gly Ser Ile Gly Glu Asn Ile Thr Glu Asn Leu Lys 
    130                 135                 140                 


Arg Val Lys Asp Ile Pro Leu Thr Leu Pro Glu Glu Leu Asp Ile Thr 
145                 150                 155                 160 


Val Arg Gly Glu Cys Tyr Met Pro Arg Ala Ser Phe Asp Gln Val Asn 
                165                 170                 175     


Gln Ala Arg Gln Glu Asn Gly Glu Pro Glu Phe Ala Asn Pro Arg Asn 
            180                 185                 190         


Ala Ala Ala Gly Thr Leu Arg Gln Leu Asp Thr Ala Val Val Ala Lys 
        195                 200                 205             


Arg Asn Leu Ala Thr Phe Leu Tyr Gln Glu Ala Ser Pro Ser Thr Arg 
    210                 215                 220                 


Asp Ser Gln Glu Lys Gly Leu Lys Tyr Leu Glu Gln Leu Gly Phe Val 
225                 230                 235                 240 


Val Asn Pro Lys Arg Ile Leu Ala Glu Asn Ile Asp Glu Ile Trp Asn 
                245                 250                 255     


Phe Ile Gln Glu Val Gly Gln Glu Arg Glu Asn Leu Pro Tyr Asp Ile 
            260                 265                 270         


Asp Gly Val Val Ile Lys Val Asn Asp Leu Ala Ser Gln Glu Glu Leu 
        275                 280                 285             


Gly Phe Thr Val Lys Ala Pro Lys Trp Ala Val Ala Tyr Lys Phe Pro 
    290                 295                 300                 


Ala Glu Glu Lys Glu Ala Gln Leu Leu Ser Val Asp Trp Thr Val Gly 
305                 310                 315                 320 


Arg Thr Gly Val Val Thr Pro Thr Ala Asn Leu Thr Pro Val Gln Leu 
                325                 330                 335     


Ala Gly Thr Thr Val Ser Arg Ala Thr Leu His Asn Val Asp Tyr Ile 
            340                 345                 350         


Ala Glu Lys Asp Ile Arg Lys Asp Asp Thr Val Ile Val Tyr Lys Ala 
        355                 360                 365             


Gly Asp Ile Ile Pro Ala Val Leu Arg Val Val Glu Ser Lys Arg Val 
    370                 375                 380                 


Ser Glu Glu Lys Leu Asp Ile Pro Thr Asn Cys Pro Ser Cys Asn Ser 
385                 390                 395                 400 


Asp Leu Leu His Phe Glu Asp Glu Val Ala Leu Arg Cys Ile Asn Pro 
                405                 410                 415     


Arg Cys Pro Ala Gln Ile Met Glu Gly Leu Ile His Phe Ala Ser Arg 
            420                 425                 430         


Asp Ala Met Asn Ile Thr Gly Leu Gly Pro Ser Ile Val Glu Lys Leu 
        435                 440                 445             


Phe Ala Ala Asn Leu Val Lys Asp Val Ala Asp Ile Tyr Arg Leu Gln 
    450                 455                 460                 


Glu Glu Asp Phe Leu Leu Leu Glu Gly Val Lys Glu Lys Ser Ala Ala 
465                 470                 475                 480 


Lys Leu Tyr Gln Ala Ile Gln Ala Ser Lys Glu Asn Ser Ala Glu Lys 
                485                 490                 495     


Leu Leu Phe Gly Leu Gly Ile Arg His Val Gly Ser Lys Ala Ser Gln 
            500                 505                 510         


Leu Leu Leu Gln Tyr Phe His Ser Ile Glu Asn Leu Tyr Gln Ala Asp 
        515                 520                 525             


Ser Glu Glu Val Ala Ser Ile Glu Ser Leu Gly Gly Val Ile Ala Lys 
    530                 535                 540                 


Ser Leu Gln Thr Tyr Phe Ala Thr Glu Gly Ser Glu Ile Leu Leu Arg 
545                 550                 555                 560 


Glu Leu Lys Glu Thr Gly Val Asn Leu Asp Tyr Lys Gly Gln Thr Val 
                565                 570                 575     


Val Ala Asp Ala Ala Leu Ser Gly Leu Thr Val Val Leu Thr Gly Lys 
            580                 585                 590         


Leu Glu Arg Leu Lys Arg Ser Glu Ala Lys Ser Lys Leu Glu Ser Leu 
        595                 600                 605             


Gly Ala Lys Val Thr Gly Ser Val Ser Lys Lys Thr Asp Leu Val Val 
    610                 615                 620                 


Val Gly Ala Asp Ala Gly Ser Lys Leu Gln Lys Ala Gln Glu Leu Gly 
625                 630                 635                 640 


Ile Gln Val Arg Asp Glu Ala Trp Leu Glu Ser Leu 
                645                 650         


