                         SEQUENCE LISTING

<110>  Howard Hughes Medical Institute
 
<120>  MODIFIED LIGAND-GATED ION CHANNELS AND METHODS OF USE

<130>  30872-0014WO1

<150>  US 62/359,534
<151>  2016-07-07

<150>  US 62/486,779
<151>  2017-04-18

<160>  12    

<170>  PatentIn version 3.5

<210>  1
<211>  224
<212>  PRT
<213>  human

<400>  1

Met Arg Cys Ser Pro Gly Gly Val Trp Leu Ala Leu Ala Ala Ser Leu 
1               5                   10                  15      


Leu His Val Ser Leu Gln Gly Glu Phe Gln Arg Lys Leu Tyr Lys Glu 
            20                  25                  30          


Leu Val Lys Asn Tyr Asn Pro Leu Glu Arg Pro Val Ala Asn Asp Ser 
        35                  40                  45              


Gln Pro Leu Thr Val Tyr Phe Ser Leu Ser Leu Leu Gln Ile Met Asp 
    50                  55                  60                  


Val Asp Glu Lys Asn Gln Val Leu Thr Thr Asn Ile Trp Leu Gln Met 
65                  70                  75                  80  


Ser Trp Thr Asp His Tyr Leu Gln Trp Asn Val Ser Glu Tyr Pro Gly 
                85                  90                  95      


Val Lys Thr Val Arg Phe Pro Asp Gly Gln Ile Trp Lys Pro Asp Ile 
            100                 105                 110         


Leu Leu Tyr Asn Ser Ala Asp Glu Arg Phe Asp Ala Thr Phe His Thr 
        115                 120                 125             


Asn Val Leu Val Asn Ser Ser Gly His Cys Gln Tyr Leu Pro Pro Gly 
    130                 135                 140                 


Ile Phe Lys Ser Ser Cys Tyr Ile Asp Val Arg Trp Phe Pro Phe Asp 
145                 150                 155                 160 


Val Gln His Cys Lys Leu Lys Phe Gly Ser Trp Ser Tyr Gly Gly Trp 
                165                 170                 175     


Ser Leu Asp Leu Gln Met Gln Glu Ala Asp Ile Ser Gly Tyr Ile Pro 
            180                 185                 190         


Asn Gly Glu Trp Asp Leu Val Gly Ile Pro Gly Lys Arg Ser Glu Arg 
        195                 200                 205             


Phe Tyr Glu Cys Cys Lys Glu Pro Tyr Pro Asp Val Thr Phe Thr Val 
    210                 215                 220                 


<210>  2
<211>  229
<212>  PRT
<213>  human

<400>  2

Met Arg Cys Ser Pro Gly Gly Val Trp Leu Ala Leu Ala Ala Ser Leu 
1               5                   10                  15      


Leu His Val Ser Leu Gln Gly Glu Phe Gln Arg Lys Leu Tyr Lys Glu 
            20                  25                  30          


Leu Val Lys Asn Tyr Asn Pro Leu Glu Arg Pro Val Ala Asn Asp Ser 
        35                  40                  45              


Gln Pro Leu Thr Val Tyr Phe Ser Leu Ser Leu Leu Gln Ile Met Asp 
    50                  55                  60                  


Val Asp Glu Lys Asn Gln Val Leu Thr Thr Asn Ile Trp Leu Gln Met 
65                  70                  75                  80  


Ser Trp Thr Asp His Tyr Leu Gln Trp Asn Val Ser Glu Tyr Pro Gly 
                85                  90                  95      


Val Lys Thr Val Arg Phe Pro Asp Gly Gln Ile Trp Lys Pro Asp Ile 
            100                 105                 110         


Leu Leu Tyr Asn Ser Ala Asp Glu Arg Phe Asp Ala Thr Phe His Thr 
        115                 120                 125             


Asn Val Leu Val Asn Ser Ser Gly His Cys Gln Tyr Leu Pro Pro Gly 
    130                 135                 140                 


Ile Phe Lys Ser Ser Cys Tyr Ile Asp Val Arg Trp Phe Pro Phe Asp 
145                 150                 155                 160 


Val Gln His Cys Lys Leu Lys Phe Gly Ser Trp Ser Tyr Gly Gly Trp 
                165                 170                 175     


Ser Leu Asp Leu Gln Met Gln Glu Ala Asp Ile Ser Gly Tyr Ile Pro 
            180                 185                 190         


Asn Gly Glu Trp Asp Leu Val Gly Ile Pro Gly Lys Arg Ser Glu Arg 
        195                 200                 205             


Phe Tyr Glu Cys Cys Lys Glu Pro Tyr Pro Asp Val Thr Phe Thr Val 
    210                 215                 220                 


Thr Met Arg Arg Arg 
225                 


<210>  3
<211>  247
<212>  PRT
<213>  mouse

<400>  3

Ile Ile Arg Arg Arg Pro Leu Phe Tyr Ala Val Ser Leu Leu Leu Pro 
1               5                   10                  15      


Ser Ile Phe Leu Met Val Val Asp Ile Val Gly Phe Cys Leu Pro Pro 
            20                  25                  30          


Asp Ser Gly Glu Arg Val Ser Phe Lys Ile Thr Leu Leu Leu Gly Tyr 
        35                  40                  45              


Ser Val Phe Leu Ile Ile Val Ser Asp Thr Leu Pro Ala Thr Ile Gly 
    50                  55                  60                  


Thr Pro Leu Ile Gly Val Tyr Phe Val Val Cys Met Ala Leu Leu Val 
65                  70                  75                  80  


Ile Ser Leu Ala Glu Thr Ile Phe Ile Val Arg Leu Val His Lys Gln 
                85                  90                  95      


Asp Leu Gln Arg Pro Val Pro Asp Trp Leu Arg His Leu Val Leu Asp 
            100                 105                 110         


Arg Ile Ala Trp Ile Leu Cys Leu Gly Glu Gln Pro Met Ala His Arg 
        115                 120                 125             


Pro Pro Ala Thr Phe Gln Ala Asn Lys Thr Asp Asp Cys Ser Gly Ser 
    130                 135                 140                 


Asp Leu Leu Pro Ala Met Gly Asn His Cys Ser His Val Gly Gly Pro 
145                 150                 155                 160 


Gln Asp Leu Glu Lys Thr Pro Arg Gly Arg Gly Ser Pro Leu Pro Pro 
                165                 170                 175     


Pro Arg Glu Ala Ser Leu Ala Val Arg Gly Leu Leu Gln Glu Leu Ser 
            180                 185                 190         


Ser Ile Arg His Phe Leu Glu Lys Arg Asp Glu Met Arg Glu Val Ala 
        195                 200                 205             


Arg Asp Trp Leu Arg Val Gly Tyr Val Leu Asp Arg Leu Leu Phe Arg 
    210                 215                 220                 


Ile Tyr Leu Leu Ala Val Leu Ala Tyr Ser Ile Thr Leu Val Thr Leu 
225                 230                 235                 240 


Trp Ser Ile Trp His Tyr Ser 
                245         


<210>  4
<211>  236
<212>  PRT
<213>  human

<400>  4

Leu Phe Tyr Val Val Ser Leu Leu Leu Pro Ser Ile Phe Leu Met Val 
1               5                   10                  15      


Met Asp Ile Val Gly Phe Tyr Leu Pro Pro Asn Ser Gly Glu Arg Val 
            20                  25                  30          


Ser Phe Lys Ile Thr Leu Leu Leu Gly Tyr Ser Val Phe Leu Ile Ile 
        35                  40                  45              


Val Ser Asp Thr Leu Pro Ala Thr Ala Ile Gly Thr Pro Leu Ile Gly 
    50                  55                  60                  


Val Tyr Phe Val Val Cys Met Ala Leu Leu Val Ile Ser Leu Ala Glu 
65                  70                  75                  80  


Thr Ile Phe Ile Val Arg Leu Val His Lys Gln Asp Leu Gln Gln Pro 
                85                  90                  95      


Val Pro Ala Trp Leu Arg His Leu Val Leu Glu Arg Ile Ala Trp Leu 
            100                 105                 110         


Leu Cys Leu Arg Glu Gln Ser Thr Ser Gln Arg Pro Pro Ala Thr Ser 
        115                 120                 125             


Gln Ala Thr Lys Thr Asp Asp Cys Ser Ala Met Gly Asn His Cys Ser 
    130                 135                 140                 


His Met Gly Gly Pro Gln Asp Phe Glu Lys Ser Pro Arg Asp Arg Cys 
145                 150                 155                 160 


Ser Pro Pro Pro Pro Pro Arg Glu Ala Ser Leu Ala Val Cys Gly Leu 
                165                 170                 175     


Leu Gln Glu Leu Ser Ser Ile Arg Gln Phe Leu Glu Lys Arg Asp Glu 
            180                 185                 190         


Ile Arg Glu Val Ala Arg Asp Trp Leu Arg Val Gly Ser Val Leu Asp 
        195                 200                 205             


Lys Leu Leu Phe His Ile Tyr Leu Leu Ala Val Leu Ala Tyr Ser Ile 
    210                 215                 220                 


Thr Leu Val Met Leu Trp Ser Ile Trp Gln Tyr Ala 
225                 230                 235     


<210>  5
<211>  202
<212>  PRT
<213>  mouse

<400>  5

Met Gly Tyr Tyr Leu Ile Gln Met Tyr Ile Pro Ser Leu Leu Ile Val 
1               5                   10                  15      


Ile Leu Ser Trp Ile Ser Phe Trp Ile Asn Met Asp Ala Ala Pro Ala 
            20                  25                  30          


Arg Val Gly Leu Gly Ile Thr Thr Val Leu Thr Met Thr Thr Gln Ser 
        35                  40                  45              


Ser Gly Ser Arg Ala Ser Leu Pro Lys Val Ser Tyr Val Lys Ala Ile 
    50                  55                  60                  


Asp Ile Trp Met Ala Val Cys Leu Leu Phe Val Phe Ser Ala Leu Leu 
65                  70                  75                  80  


Glu Tyr Ala Ala Val Asn Phe Val Ser Arg Gln His Lys Glu Leu Leu 
                85                  90                  95      


Arg Phe Arg Arg Lys Arg Arg His His Lys Glu Asp Glu Ala Gly Glu 
            100                 105                 110         


Gly Arg Phe Asn Phe Ser Ala Tyr Gly Met Gly Pro Ala Cys Leu Gln 
        115                 120                 125             


Ala Lys Asp Gly Ile Ser Val Lys Gly Ala Asn Asn Ser Asn Thr Thr 
    130                 135                 140                 


Asn Pro Pro Pro Ala Pro Ser Lys Ser Pro Glu Glu Met Arg Lys Leu 
145                 150                 155                 160 


Phe Ile Gln Arg Ala Lys Lys Ile Asp Lys Ile Ser Arg Ile Gly Phe 
                165                 170                 175     


Pro Met Ala Phe Leu Ile Phe Asn Met Phe Tyr Trp Ile Ile Tyr Lys 
            180                 185                 190         


Ile Val Arg Arg Glu Asp Val His Asn Gln 
        195                 200         


<210>  6
<211>  471
<212>  PRT
<213>  artificial

<220>
<223>  (alpha)7-5HT3 chimeric receptor

<400>  6

Met Arg Cys Ser Pro Gly Gly Val Trp Leu Ala Leu Ala Ala Ser Leu 
1               5                   10                  15      


Leu His Val Ser Leu Gln Gly Glu Phe Gln Arg Lys Leu Tyr Lys Glu 
            20                  25                  30          


Leu Val Lys Asn Tyr Asn Pro Leu Glu Arg Pro Val Ala Asn Asp Ser 
        35                  40                  45              


Gln Pro Leu Thr Val Tyr Phe Ser Leu Ser Leu Leu Gln Ile Met Asp 
    50                  55                  60                  


Val Asp Glu Lys Asn Gln Val Leu Thr Thr Asn Ile Trp Leu Gln Met 
65                  70                  75                  80  


Ser Trp Thr Asp His Tyr Leu Gln Trp Asn Val Ser Glu Tyr Pro Gly 
                85                  90                  95      


Val Lys Thr Val Arg Phe Pro Asp Gly Gln Ile Trp Lys Pro Asp Ile 
            100                 105                 110         


Leu Leu Tyr Asn Ser Ala Asp Glu Arg Phe Asp Ala Thr Phe His Thr 
        115                 120                 125             


Asn Val Leu Val Asn Ser Ser Gly His Cys Gln Tyr Leu Pro Pro Gly 
    130                 135                 140                 


Ile Phe Lys Ser Ser Cys Tyr Ile Asp Val Arg Trp Phe Pro Phe Asp 
145                 150                 155                 160 


Val Gln His Cys Lys Leu Lys Phe Gly Ser Trp Ser Tyr Gly Gly Trp 
                165                 170                 175     


Ser Leu Asp Leu Gln Met Gln Glu Ala Asp Ile Ser Gly Tyr Ile Pro 
            180                 185                 190         


Asn Gly Glu Trp Asp Leu Val Gly Ile Pro Gly Lys Arg Ser Glu Arg 
        195                 200                 205             


Phe Tyr Glu Cys Cys Lys Glu Pro Tyr Pro Asp Val Thr Phe Thr Val 
    210                 215                 220                 


Ile Ile Arg Arg Arg Pro Leu Phe Tyr Ala Val Ser Leu Leu Leu Pro 
225                 230                 235                 240 


Ser Ile Phe Leu Met Val Val Asp Ile Val Gly Phe Cys Leu Pro Pro 
                245                 250                 255     


Asp Ser Gly Glu Arg Val Ser Phe Lys Ile Thr Leu Leu Leu Gly Tyr 
            260                 265                 270         


Ser Val Phe Leu Ile Ile Val Ser Asp Thr Leu Pro Ala Thr Ile Gly 
        275                 280                 285             


Thr Pro Leu Ile Gly Val Tyr Phe Val Val Cys Met Ala Leu Leu Val 
    290                 295                 300                 


Ile Ser Leu Ala Glu Thr Ile Phe Ile Val Arg Leu Val His Lys Gln 
305                 310                 315                 320 


Asp Leu Gln Arg Pro Val Pro Asp Trp Leu Arg His Leu Val Leu Asp 
                325                 330                 335     


Arg Ile Ala Trp Ile Leu Cys Leu Gly Glu Gln Pro Met Ala His Arg 
            340                 345                 350         


Pro Pro Ala Thr Phe Gln Ala Asn Lys Thr Asp Asp Cys Ser Gly Ser 
        355                 360                 365             


Asp Leu Leu Pro Ala Met Gly Asn His Cys Ser His Val Gly Gly Pro 
    370                 375                 380                 


Gln Asp Leu Glu Lys Thr Pro Arg Gly Arg Gly Ser Pro Leu Pro Pro 
385                 390                 395                 400 


Pro Arg Glu Ala Ser Leu Ala Val Arg Gly Leu Leu Gln Glu Leu Ser 
                405                 410                 415     


Ser Ile Arg His Phe Leu Glu Lys Arg Asp Glu Met Arg Glu Val Ala 
            420                 425                 430         


Arg Asp Trp Leu Arg Val Gly Tyr Val Leu Asp Arg Leu Leu Phe Arg 
        435                 440                 445             


Ile Tyr Leu Leu Ala Val Leu Ala Tyr Ser Ile Thr Leu Val Thr Leu 
    450                 455                 460                 


Trp Ser Ile Trp His Tyr Ser 
465                 470     


<210>  7
<211>  430
<212>  PRT
<213>  artificial

<220>
<223>  (alpha)7-GlyR chimeric receptor

<400>  7

Met Arg Cys Ser Pro Gly Gly Val Trp Leu Ala Leu Ala Ala Ser Leu 
1               5                   10                  15      


Leu His Val Ser Leu Gln Gly Glu Phe Gln Arg Lys Leu Tyr Lys Glu 
            20                  25                  30          


Leu Val Lys Asn Tyr Asn Pro Leu Glu Arg Pro Val Ala Asn Asp Ser 
        35                  40                  45              


Gln Pro Leu Thr Val Tyr Phe Ser Leu Ser Leu Leu Gln Ile Met Asp 
    50                  55                  60                  


Val Asp Glu Lys Asn Gln Val Leu Thr Thr Asn Ile Trp Leu Gln Met 
65                  70                  75                  80  


Ser Trp Thr Asp His Tyr Leu Gln Trp Asn Val Ser Glu Tyr Pro Gly 
                85                  90                  95      


Val Lys Thr Arg Phe Pro Asp Gly Gln Ile Trp Lys Pro Asp Ile Leu 
            100                 105                 110         


Leu Tyr Asn Ser Ala Asp Glu Arg Phe Asp Ala Thr Phe His Thr Asn 
        115                 120                 125             


Val Leu Val Asn Ser Ser Gly His Cys Gln Tyr Leu Pro Pro Gly Ile 
    130                 135                 140                 


Phe Lys Ser Ser Cys Tyr Ile Asp Val Arg Trp Phe Pro Phe Asp Val 
145                 150                 155                 160 


Gln His Cys Lys Leu Lys Phe Gly Ser Trp Ser Tyr Gly Gly Trp Ser 
                165                 170                 175     


Leu Asp Leu Gln Met Gln Glu Ala Asp Ile Ser Gly Tyr Ile Pro Asn 
            180                 185                 190         


Gly Glu Trp Asp Leu Val Gly Ile Pro Gly Lys Arg Ser Glu Arg Phe 
        195                 200                 205             


Tyr Glu Cys Cys Lys Glu Pro Tyr Pro Asp Val Thr Phe Thr Val Thr 
    210                 215                 220                 


Met Arg Arg Arg Met Gly Tyr Tyr Leu Ile Gln Met Tyr Ile Pro Ser 
225                 230                 235                 240 


Leu Leu Ile Val Ile Leu Ser Trp Ile Ser Phe Trp Ile Asn Met Asp 
                245                 250                 255     


Ala Ala Pro Ala Arg Val Gly Leu Gly Ile Thr Thr Val Leu Thr Met 
            260                 265                 270         


Thr Thr Gln Ser Ser Gly Ser Arg Ala Ser Leu Pro Lys Val Ser Tyr 
        275                 280                 285             


Val Lys Ala Ile Asp Ile Trp Met Ala Val Cys Leu Leu Phe Val Phe 
    290                 295                 300                 


Ser Ala Leu Leu Glu Tyr Ala Ala Val Asn Phe Val Ser Arg Gln His 
305                 310                 315                 320 


Lys Glu Leu Leu Arg Phe Arg Arg Lys Arg Arg His His Lys Glu Asp 
                325                 330                 335     


Glu Ala Gly Glu Gly Arg Phe Asn Phe Ser Ala Tyr Gly Met Gly Pro 
            340                 345                 350         


Ala Cys Leu Gln Ala Lys Asp Gly Ile Ser Val Lys Gly Ala Asn Asn 
        355                 360                 365             


Ser Asn Thr Thr Asn Pro Pro Pro Ala Pro Ser Lys Ser Pro Glu Glu 
    370                 375                 380                 


Met Arg Lys Leu Phe Ile Gln Arg Ala Lys Lys Ile Asp Lys Ile Ser 
385                 390                 395                 400 


Arg Ile Gly Phe Pro Met Ala Phe Leu Ile Phe Asn Met Phe Tyr Trp 
                405                 410                 415     


Ile Ile Tyr Lys Ile Val Arg Arg Glu Asp Val His Asn Gln 
            420                 425                 430 


<210>  8
<211>  466
<212>  PRT
<213>  artificial

<220>
<223>  (alpha)7-5HT3 chimeric receptor

<400>  8

Met Arg Cys Ser Pro Gly Gly Val Trp Leu Ala Leu Ala Ala Ser Leu 
1               5                   10                  15      


Leu His Val Ser Leu Gln Gly Glu Phe Gln Arg Lys Leu Tyr Lys Glu 
            20                  25                  30          


Leu Val Lys Asn Tyr Asn Pro Leu Glu Arg Pro Val Ala Asn Asp Ser 
        35                  40                  45              


Gln Pro Leu Thr Val Tyr Phe Ser Leu Ser Leu Leu Gln Ile Met Asp 
    50                  55                  60                  


Val Asp Glu Lys Asn Gln Val Leu Thr Thr Asn Ile Trp Leu Gln Met 
65                  70                  75                  80  


Ser Trp Thr Asp His Tyr Leu Gln Trp Asn Val Ser Glu Tyr Pro Gly 
                85                  90                  95      


Val Lys Thr Val Arg Phe Pro Asp Gly Gln Ile Trp Lys Pro Asp Ile 
            100                 105                 110         


Leu Leu Tyr Asn Ser Ala Asp Glu Arg Phe Asp Ala Thr Phe His Thr 
        115                 120                 125             


Asn Val Leu Val Asn Ser Ser Gly His Cys Gln Tyr Leu Pro Pro Gly 
    130                 135                 140                 


Ile Phe Lys Ser Ser Cys Tyr Ile Asp Val Arg Trp Phe Pro Phe Asp 
145                 150                 155                 160 


Val Gln His Cys Lys Leu Lys Phe Gly Ser Trp Ser Tyr Gly Gly Trp 
                165                 170                 175     


Ser Leu Asp Leu Gln Met Gln Glu Ala Asp Ile Ser Gly Tyr Ile Pro 
            180                 185                 190         


Asn Gly Glu Trp Asp Leu Val Gly Ile Pro Gly Lys Arg Ser Glu Arg 
        195                 200                 205             


Phe Tyr Glu Cys Cys Lys Glu Pro Tyr Pro Asp Val Thr Phe Thr Val 
    210                 215                 220                 


Ile Ile Arg Arg Arg Pro Leu Phe Tyr Val Val Ser Leu Leu Leu Pro 
225                 230                 235                 240 


Ser Ile Phe Leu Met Val Met Asp Ile Val Gly Phe Tyr Leu Pro Pro 
                245                 250                 255     


Asn Ser Gly Glu Arg Val Ser Phe Lys Ile Thr Leu Leu Leu Gly Tyr 
            260                 265                 270         


Ser Val Phe Leu Ile Ile Val Ser Asp Thr Leu Pro Ala Thr Ala Ile 
        275                 280                 285             


Gly Thr Pro Leu Ile Gly Val Tyr Phe Val Val Cys Met Ala Leu Leu 
    290                 295                 300                 


Val Ile Ser Leu Ala Glu Thr Ile Phe Ile Val Arg Leu Val His Lys 
305                 310                 315                 320 


Gln Asp Leu Gln Gln Pro Val Pro Ala Trp Leu Arg His Leu Val Leu 
                325                 330                 335     


Glu Arg Ile Ala Trp Leu Leu Cys Leu Arg Glu Gln Ser Thr Ser Gln 
            340                 345                 350         


Arg Pro Pro Ala Thr Ser Gln Ala Thr Lys Thr Asp Asp Cys Ser Ala 
        355                 360                 365             


Met Gly Asn His Cys Ser His Met Gly Gly Pro Gln Asp Phe Glu Lys 
    370                 375                 380                 


Ser Pro Arg Asp Arg Cys Ser Pro Pro Pro Pro Pro Arg Glu Ala Ser 
385                 390                 395                 400 


Leu Ala Val Cys Gly Leu Leu Gln Glu Leu Ser Ser Ile Arg Gln Phe 
                405                 410                 415     


Leu Glu Lys Arg Asp Glu Ile Arg Glu Val Ala Arg Asp Trp Leu Arg 
            420                 425                 430         


Val Gly Ser Val Leu Asp Lys Leu Leu Phe His Ile Tyr Leu Leu Ala 
        435                 440                 445             


Val Leu Ala Tyr Ser Ile Thr Leu Val Met Leu Trp Ser Ile Trp Gln 
    450                 455                 460                 


Tyr Ala 
465     


<210>  9
<211>  195
<212>  PRT
<213>  human

<400>  9

Leu Leu Gln Thr Tyr Phe Pro Ala Thr Leu Met Val Met Leu Ser Trp 
1               5                   10                  15      


Val Ser Phe Trp Ile Asp Arg Arg Ala Val Pro Ala Arg Val Pro Leu 
            20                  25                  30          


Gly Ile Thr Thr Val Leu Thr Met Ser Thr Ile Ile Thr Gly Val Asn 
        35                  40                  45              


Ala Ser Met Pro Arg Val Ser Tyr Ile Lys Ala Val Asp Ile Tyr Leu 
    50                  55                  60                  


Trp Val Ser Phe Val Phe Val Phe Leu Ser Val Leu Glu Tyr Ala Ala 
65                  70                  75                  80  


Val Asn Tyr Leu Thr Thr Val Gln Glu Arg Lys Glu Gln Lys Leu Arg 
                85                  90                  95      


Glu Lys Leu Pro Cys Thr Ser Gly Leu Pro Pro Pro Arg Thr Ala Met 
            100                 105                 110         


Leu Asp Gly Asn Tyr Ser Asp Gly Glu Val Asn Asp Leu Asp Asn Tyr 
        115                 120                 125             


Met Pro Glu Asn Gly Glu Lys Pro Asp Arg Met Met Val Gln Leu Thr 
    130                 135                 140                 


Leu Ala Ser Glu Arg Ser Ser Pro Gln Arg Lys Ser Gln Arg Ser Ser 
145                 150                 155                 160 


Tyr Val Ser Met Arg Ile Asp Thr His Ala Ile Asp Lys Tyr Ser Arg 
                165                 170                 175     


Ile Ile Phe Pro Ala Ala Tyr Ile Leu Phe Asn Leu Ile Tyr Trp Ser 
            180                 185                 190         


Ile Phe Ser 
        195 


<210>  10
<211>  428
<212>  PRT
<213>  artificial

<220>
<223>  (alpha)7- GABAC chimeric receptor

<400>  10

Met Arg Cys Ser Pro Gly Gly Val Trp Leu Ala Leu Ala Ala Ser Leu 
1               5                   10                  15      


Leu His Val Ser Leu Gln Gly Glu Phe Gln Arg Lys Leu Tyr Lys Glu 
            20                  25                  30          


Leu Val Lys Asn Tyr Asn Pro Leu Glu Arg Pro Val Ala Asn Asp Ser 
        35                  40                  45              


Gln Pro Leu Thr Val Tyr Phe Ser Leu Ser Leu Leu Gln Ile Met Asp 
    50                  55                  60                  


Val Asp Glu Lys Asn Gln Val Leu Thr Thr Asn Ile Trp Leu Gln Met 
65                  70                  75                  80  


Ser Trp Thr Asp His Tyr Leu Gln Trp Asn Val Ser Glu Tyr Pro Gly 
                85                  90                  95      


Val Lys Thr Val Arg Phe Pro Asp Gly Gln Ile Trp Lys Pro Asp Ile 
            100                 105                 110         


Leu Leu Tyr Asn Ser Ala Asp Glu Arg Phe Asp Ala Thr Phe His Thr 
        115                 120                 125             


Asn Val Leu Val Asn Ser Ser Gly His Cys Gln Tyr Leu Pro Pro Gly 
    130                 135                 140                 


Ile Phe Lys Ser Ser Cys Tyr Ile Asp Val Arg Trp Phe Pro Phe Asp 
145                 150                 155                 160 


Val Gln His Cys Lys Leu Lys Phe Gly Ser Trp Ser Tyr Gly Gly Trp 
                165                 170                 175     


Ser Leu Asp Leu Gln Met Gln Glu Ala Asp Ile Ser Gly Tyr Ile Pro 
            180                 185                 190         


Asn Gly Glu Trp Asp Leu Val Gly Ile Pro Gly Lys Arg Ser Glu Arg 
        195                 200                 205             


Phe Tyr Glu Cys Cys Lys Glu Pro Tyr Pro Asp Val Thr Phe Thr Val 
    210                 215                 220                 


Thr Met Arg Arg Arg Thr Leu Tyr Tyr Leu Leu Gln Thr Tyr Phe Pro 
225                 230                 235                 240 


Ala Thr Leu Met Val Met Leu Ser Trp Val Ser Phe Trp Ile Asp Arg 
                245                 250                 255     


Arg Ala Val Pro Ala Arg Val Pro Leu Gly Ile Thr Thr Val Leu Thr 
            260                 265                 270         


Met Ser Thr Ile Ile Thr Gly Val Asn Ala Ser Met Pro Arg Val Ser 
        275                 280                 285             


Tyr Ile Lys Ala Val Asp Ile Tyr Leu Trp Val Ser Phe Val Phe Val 
    290                 295                 300                 


Phe Leu Ser Val Leu Glu Tyr Ala Ala Val Asn Tyr Leu Thr Thr Val 
305                 310                 315                 320 


Gln Glu Arg Lys Glu Gln Lys Leu Arg Glu Lys Leu Pro Cys Thr Ser 
                325                 330                 335     


Gly Leu Pro Pro Pro Arg Thr Ala Met Leu Asp Gly Asn Tyr Ser Asp 
            340                 345                 350         


Gly Glu Val Asn Asp Leu Asp Asn Tyr Met Pro Glu Asn Gly Glu Lys 
        355                 360                 365             


Pro Asp Arg Met Met Val Gln Leu Thr Leu Ala Ser Glu Arg Ser Ser 
    370                 375                 380                 


Pro Gln Arg Lys Ser Gln Arg Ser Ser Tyr Val Ser Met Arg Ile Asp 
385                 390                 395                 400 


Thr His Ala Ile Asp Lys Tyr Ser Arg Ile Ile Phe Pro Ala Ala Tyr 
                405                 410                 415     


Ile Leu Phe Asn Leu Ile Tyr Trp Ser Ile Phe Ser 
            420                 425             


<210>  11
<211>  233
<212>  PRT
<213>  human

<400>  11

Met Arg Cys Ser Pro Gly Gly Val Trp Leu Ala Leu Ala Ala Ser Leu 
1               5                   10                  15      


Leu His Val Ser Leu Gln Gly Glu Phe Gln Arg Lys Leu Tyr Lys Glu 
            20                  25                  30          


Leu Val Lys Asn Tyr Asn Pro Leu Glu Arg Pro Val Ala Asn Asp Ser 
        35                  40                  45              


Gln Pro Leu Thr Val Tyr Phe Ser Leu Ser Leu Leu Gln Ile Met Asp 
    50                  55                  60                  


Val Asp Glu Lys Asn Gln Val Leu Thr Thr Asn Ile Trp Leu Gln Met 
65                  70                  75                  80  


Ser Trp Thr Asp His Tyr Leu Gln Trp Asn Val Ser Glu Tyr Pro Gly 
                85                  90                  95      


Val Lys Thr Val Arg Phe Pro Asp Gly Gln Ile Trp Lys Pro Asp Ile 
            100                 105                 110         


Leu Leu Tyr Asn Ser Ala Asp Glu Arg Phe Asp Ala Thr Phe His Thr 
        115                 120                 125             


Asn Val Leu Val Asn Ser Ser Gly His Cys Gln Tyr Leu Pro Pro Gly 
    130                 135                 140                 


Ile Phe Lys Ser Ser Cys Tyr Ile Asp Val Arg Trp Phe Pro Phe Asp 
145                 150                 155                 160 


Val Gln His Cys Lys Leu Lys Phe Gly Ser Trp Ser Tyr Gly Gly Trp 
                165                 170                 175     


Ser Leu Asp Leu Gln Met Gln Glu Ala Asp Ile Ser Gly Tyr Ile Pro 
            180                 185                 190         


Asn Gly Glu Trp Asp Leu Val Gly Ile Pro Gly Lys Arg Ser Glu Arg 
        195                 200                 205             


Phe Tyr Glu Cys Cys Lys Glu Pro Tyr Pro Asp Val Thr Phe Thr Val 
    210                 215                 220                 


Thr Met Arg Arg Arg Thr Leu Tyr Tyr 
225                 230             


<210>  12
<211>  502
<212>  PRT
<213>  rat

<400>  12

Met Gly Gly Gly Arg Gly Gly Ile Trp Leu Ala Leu Ala Ala Ala Leu 
1               5                   10                  15      


Leu His Val Ser Leu Gln Gly Glu Phe Gln Arg Arg Leu Tyr Lys Glu 
            20                  25                  30          


Leu Val Lys Asn Tyr Asn Pro Leu Glu Arg Pro Val Ala Asn Asp Ser 
        35                  40                  45              


Gln Pro Leu Thr Val Tyr Phe Ser Leu Ser Leu Leu Gln Ile Met Asp 
    50                  55                  60                  


Val Asp Glu Lys Asn Gln Val Leu Thr Thr Asn Ile Trp Leu Gln Met 
65                  70                  75                  80  


Ser Trp Thr Asp His Tyr Leu Gln Trp Asn Met Ser Glu Tyr Pro Gly 
                85                  90                  95      


Val Lys Asn Val Arg Phe Pro Asp Gly Gln Ile Trp Lys Pro Asp Ile 
            100                 105                 110         


Leu Leu Tyr Asn Ser Ala Asp Glu Arg Phe Asp Ala Thr Phe His Thr 
        115                 120                 125             


Asn Val Leu Val Asn Ala Ser Gly His Cys Gln Tyr Leu Pro Pro Gly 
    130                 135                 140                 


Ile Phe Lys Ser Ser Cys Tyr Ile Asp Val Arg Trp Phe Pro Phe Asp 
145                 150                 155                 160 


Val Gln Gln Cys Lys Leu Lys Phe Gly Ser Trp Ser Tyr Gly Gly Trp 
                165                 170                 175     


Ser Leu Asp Leu Gln Met Gln Glu Ala Asp Ile Ser Ser Tyr Ile Pro 
            180                 185                 190         


Asn Gly Glu Trp Asp Leu Met Gly Ile Pro Gly Lys Arg Asn Glu Lys 
        195                 200                 205             


Phe Tyr Glu Cys Cys Lys Glu Pro Tyr Pro Asp Val Thr Tyr Thr Val 
    210                 215                 220                 


Thr Met Arg Arg Arg Thr Leu Tyr Tyr Gly Leu Asn Leu Leu Ile Pro 
225                 230                 235                 240 


Cys Val Leu Ile Ser Ala Leu Ala Leu Leu Val Phe Leu Leu Pro Ala 
                245                 250                 255     


Asp Ser Gly Glu Lys Ile Ser Leu Gly Ile Thr Val Leu Leu Ser Leu 
            260                 265                 270         


Thr Val Phe Met Leu Leu Val Ala Glu Ile Met Pro Ala Thr Ser Asp 
        275                 280                 285             


Ser Val Pro Leu Ile Ala Gln Tyr Phe Ala Ser Thr Met Ile Ile Val 
    290                 295                 300                 


Gly Leu Ser Val Val Val Thr Val Ile Val Leu Arg Tyr His His His 
305                 310                 315                 320 


Asp Pro Asp Gly Gly Lys Met Pro Lys Trp Thr Arg Ile Ile Leu Leu 
                325                 330                 335     


Asn Trp Cys Ala Trp Phe Leu Arg Met Lys Arg Pro Gly Glu Asp Lys 
            340                 345                 350         


Val Arg Pro Ala Cys Gln His Lys Pro Arg Arg Cys Ser Leu Ala Ser 
        355                 360                 365             


Val Glu Leu Ser Ala Gly Ala Gly Pro Pro Thr Ser Asn Gly Asn Leu 
    370                 375                 380                 


Leu Tyr Ile Gly Phe Arg Gly Leu Glu Gly Met His Cys Ala Pro Thr 
385                 390                 395                 400 


Pro Asp Ser Gly Val Val Cys Gly Arg Leu Ala Cys Ser Pro Thr His 
                405                 410                 415     


Asp Glu His Leu Met His Gly Ala His Pro Ser Asp Gly Asp Pro Asp 
            420                 425                 430         


Leu Ala Lys Ile Leu Glu Glu Val Arg Tyr Ile Ala Asn Arg Asn Arg 
        435                 440                 445             


Cys Gln Asp Glu Ser Glu Val Ile Cys Ser Glu Trp Lys Phe Ala Ala 
    450                 455                 460                 


Cys Val Val Asp Pro Leu Cys Leu Met Ala Phe Ser Val Phe Thr Ile 
465                 470                 475                 480 


Ile Cys Thr Ile Gly Ile Leu Met Ser Ala Pro Asn Phe Val Glu Ala 
                485                 490                 495     


Val Ser Lys Asp Phe Ala 
            500         


