                         SEQUENCE LISTING

<110>  Novozymes A/S
 
<120>  PECTATE LYASE VARIANTS AND POLYNUCLEOTIDES ENCODING SAME

<130>  13260-WO-PCT

<160>  2     

<170>  PatentIn version 3.5

<210>  1
<211>  399
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  The parent pectate lyase which is a variant of the pectate lyase 
       from Bacillus subtilis disclosed in WO 2002/09274.

<400>  1

Ala Asp Leu Gly His Gln Thr Leu Glu Ser Asn Asp Gly Trp Gly Ala 
1               5                   10                  15      


Tyr Ser Thr Gly Thr Thr Gly Gly Ser Lys Ala Ser Ser Ser His Val 
            20                  25                  30          


Tyr Thr Val Ser Asn Arg Asn Gln Leu Val Ser Ala Leu Gly Lys Pro 
        35                  40                  45              


Thr Asn Thr Thr Pro Lys Ile Ile Tyr Ile Lys Gly Thr Ile Asp Phe 
    50                  55                  60                  


Asn Val Asp Asp Asn Leu Lys Pro Leu Gly Leu Asn Asp Tyr Lys Asp 
65                  70                  75                  80  


Pro Glu Tyr Asp Leu Asp Lys Tyr Leu Lys Ala Tyr Asp Pro Ser Thr 
                85                  90                  95      


Trp Gly Lys Lys Glu Pro Ser Gly Pro Leu Glu Glu Ala Arg Ala Arg 
            100                 105                 110         


Ser Gln Lys Asn Gln Lys Ala Arg Val Met Val Asp Ile Pro Ala Asn 
        115                 120                 125             


Thr Thr Ile Val Gly Ser Gly Thr Asn Ala Ile Ile Val Gly Gly Asn 
    130                 135                 140                 


Phe His Ile Lys Ser Asp Asn Val Ile Ile Arg Asn Ile Glu Phe Gln 
145                 150                 155                 160 


Asp Ala Tyr Asp Tyr Phe Pro Gln Trp Asp Pro Thr Asp Gly Ser Ser 
                165                 170                 175     


Gly Asn Trp Asn Ser Gln Tyr Asp Asn Ile Thr Ile Asn Gly Gly Thr 
            180                 185                 190         


His Ile Trp Ile Asp His Cys Thr Phe Asn Asp Gly Ser Arg Pro Asp 
        195                 200                 205             


Ser Thr Ser Pro Thr Tyr Phe Gly Arg Pro Tyr Gln His His Asp Gly 
    210                 215                 220                 


Gln Thr Asp Ala Ser Asn Gly Ala Asn Tyr Ile Thr Met Ser Tyr Asn 
225                 230                 235                 240 


Tyr Tyr His Asp His Asp Lys Ser Ser Ile Phe Gly Ser Ser Asp Ser 
                245                 250                 255     


Lys Ile Ser Asp Asp Gly Lys Leu Lys Ile Thr Leu His His Asn Arg 
            260                 265                 270         


Tyr Lys Asn Ile Val Gln Arg Ala Pro Arg Val Arg Phe Gly Gln Val 
        275                 280                 285             


His Val Tyr Asn Asn Tyr Tyr Glu Gly Ser Thr Ser Ser Ser Asp Tyr 
    290                 295                 300                 


Pro Phe Ser Tyr Ala Trp Gly Ile Gly Lys Ser Ser Lys Ile Tyr Ala 
305                 310                 315                 320 


Gln Asn Asn Val Ile Asp Val Pro Gly Leu Pro Ala Ala Lys Thr Ile 
                325                 330                 335     


Lys Val Phe Ser Gly Gly Thr Ala Leu Tyr Asp Ser Gly Thr Leu Leu 
            340                 345                 350         


Asn Gly Thr Gln Ile Asn Ala Ser Ala Ala Asn Gly Leu Ser Ser Ser 
        355                 360                 365             


Val Gly Trp Thr Pro Ser Leu His Gly Thr Ile Asp Ala Ser Ala His 
    370                 375                 380                 


Val Lys Ser Asn Val Ile Ser Gln Ala Gly Ala Gly Lys Leu Asn 
385                 390                 395                 


<210>  2
<211>  399
<212>  PRT
<213>  B. subtilis

<400>  2

Ala Asp Leu Gly His Gln Thr Leu Gly Ser Asn Asp Gly Trp Gly Ala 
1               5                   10                  15      


Tyr Ser Thr Gly Thr Thr Gly Gly Ser Lys Ala Ser Ser Ser Asn Val 
            20                  25                  30          


Tyr Thr Val Ser Asn Arg Asn Gln Leu Val Ser Ala Leu Gly Lys Glu 
        35                  40                  45              


Thr Asn Thr Thr Pro Lys Ile Ile Tyr Ile Lys Gly Thr Ile Asp Met 
    50                  55                  60                  


Asn Val Asp Asp Asn Leu Lys Pro Leu Gly Leu Asn Asp Tyr Lys Asp 
65                  70                  75                  80  


Pro Glu Tyr Asp Leu Asp Lys Tyr Leu Lys Ala Tyr Asp Pro Ser Thr 
                85                  90                  95      


Trp Gly Lys Lys Glu Pro Ser Gly Thr Gln Glu Glu Ala Arg Ala Arg 
            100                 105                 110         


Ser Gln Lys Asn Gln Lys Ala Arg Val Met Val Asp Ile Pro Ala Asn 
        115                 120                 125             


Thr Thr Ile Val Gly Ser Gly Thr Asn Ala Lys Val Val Gly Gly Asn 
    130                 135                 140                 


Phe Gln Ile Lys Ser Asp Asn Val Ile Ile Arg Asn Ile Glu Phe Gln 
145                 150                 155                 160 


Asp Ala Tyr Asp Tyr Phe Pro Gln Trp Asp Pro Thr Asp Gly Ser Ser 
                165                 170                 175     


Gly Asn Trp Asn Ser Gln Tyr Asp Asn Ile Thr Ile Asn Gly Gly Thr 
            180                 185                 190         


His Ile Trp Ile Asp His Cys Thr Phe Asn Asp Gly Ser Arg Pro Asp 
        195                 200                 205             


Ser Thr Ser Pro Lys Tyr Tyr Gly Arg Lys Tyr Gln His His Asp Gly 
    210                 215                 220                 


Gln Thr Asp Ala Ser Asn Gly Ala Asn Tyr Ile Thr Met Ser Tyr Asn 
225                 230                 235                 240 


Tyr Tyr His Asp His Asp Lys Ser Ser Ile Phe Gly Ser Ser Asp Ser 
                245                 250                 255     


Lys Thr Ser Asp Asp Gly Lys Leu Lys Ile Thr Leu His His Asn Arg 
            260                 265                 270         


Tyr Lys Asn Ile Val Gln Arg Ala Pro Arg Val Arg Phe Gly Gln Val 
        275                 280                 285             


His Val Tyr Asn Asn Tyr Tyr Glu Gly Ser Thr Ser Ser Ser Ser Tyr 
    290                 295                 300                 


Pro Phe Ser Tyr Ala Trp Gly Ile Gly Lys Ser Ser Lys Ile Tyr Ala 
305                 310                 315                 320 


Gln Asn Asn Val Ile Asp Val Pro Gly Leu Ser Ala Ala Lys Thr Ile 
                325                 330                 335     


Ser Val Phe Ser Gly Gly Thr Ala Leu Tyr Asp Ser Gly Thr Leu Leu 
            340                 345                 350         


Asn Gly Thr Gln Ile Asn Ala Ser Ala Ala Asn Gly Leu Ser Ser Ser 
        355                 360                 365             


Val Gly Trp Thr Pro Ser Leu His Gly Ser Ile Asp Ala Ser Ala Asn 
    370                 375                 380                 


Val Lys Ser Asn Val Ile Asn Gln Ala Gly Ala Gly Lys Leu Asn 
385                 390                 395                 


