                         SEQUENCE LISTING

<110>  DuPont Nutrition Biosciences ApS
       Yu, Shukun
       Shipovskov, Stepan
       Kragh, Karsten  M
 
<120>  IN FEED ASSAY OF MICROBIAL PROTEASES USING PEPTIDE SUBSTRATES

<130>  NB41020

<160>  18    

<170>  PatentIn version 3.5

<210>  1
<211>  7
<212>  PRT
<213>  artificial sequence

<220>
<223>  peptidase substrate


<220>
<221>  MISC_FEATURE
<222>  (1)..(1)
<223>  Modified with a detectable moiety

<220>
<221>  MISC_FEATURE
<222>  (3)..(3)
<223>  Xaa  is an amino acid selected from the group consisting of Leu, 
       Lys, Arg, Glu, Phe, Asp, Tyr, His, Ala, Thr, Cys, Gly and Trp

<220>
<221>  MISC_FEATURE
<222>  (4)..(4)
<223>  Xaa  is an amino acid selected from the group consisting of Phe, 
       Val, Lys, Ala, Cys, Glu, Leu, Pro, Tyr, His, Ile, Arg and Ser

<220>
<221>  MISC_FEATURE
<222>  (6)..(6)
<223>  Modified with a detectable moiety if Xaa in position 7 is absent

<220>
<221>  MISC_FEATURE
<222>  (7)..(7)
<223>  Modified with a detectable moiety if Xaa in position 7 is present

<220>
<221>  MISC_FEATURE
<222>  (7)..(7)
<223>  Xaa is K or it may be absent

<400>  1

Ala Ala Xaa Xaa Ala Ala Xaa 
1               5           

<210>  2
<211>  6
<212>  PRT
<213>  artificial sequence

<220>
<223>  peptidase substrate

<220>
<221>  MISC_FEATURE
<222>  (1)..(1)
<223>  Modified with succinoyl moiety

<220>
<221>  MISC_FEATURE
<222>  (6)..(6)
<223>  Modified with 5-Amino-2-nitrobenzoic acid moiety

<400>  2
Asp Met Phe Ile Asn Asp 
1               5       

<210>  3
<211>  6
<212>  PRT
<213>  artificial sequence

<220>
<223>  peptidase substrate

<220>
<221>  MISC_FEATURE
<222>  (1)..(1)
<223>  Modified with a detectable moiety

<220>
<221>  MISC_FEATURE
<222>  (6)..(6)
<223>  Modified with a detectable moiety

<400>  3
Ala Ala Lys Phe Ala Ala 
1               5       

<210>  4
<211>  6
<212>  PRT
<213>  artificial sequence

<220>
<223>  peptidase substrate

<220>
<221>  MISC_FEATURE
<222>  (1)..(1)
<223>  Modified with succinoyl moiety

<220>
<221>  MISC_FEATURE
<222>  (6)..(6)
<223>  Modified with a detectable moiety

<400>  4
Asp Met Ser Ile Asn Asp 
1               5       

<210>  5
<211>  4
<212>  PRT
<213>  artificial sequence

<220>
<223>  peptidase substrate

<220>
<221>  MISC_FEATURE
<222>  (1)..(1)
<223>  modified with aminobenzoyl or anthraniloyl moiety

<220>
<221>  MISC_FEATURE
<222>  (4)..(4)
<223>  modified with p-nitrobenzylamide moiety

<400>  5
Ala Gly Leu Ala 
1               

<210>  6
<211>  11
<212>  PRT
<213>  artificial sequence

<220>
<223>  peptidase substrate

<220>
<221>  MISC_FEATURE
<222>  (2)..(2)
<223>  Xaa is nitrotyrosine

<220>
<221>  MISC_FEATURE
<222>  (11)..(11)
<223>  Derivatized with aminobenzoate moiety

<400>  6
His Xaa Asp Met Phe Ile Asn Asp Lys Pro Lys 
1               5                   10      

<210>  7
<211>  6
<212>  PRT
<213>  artificial sequence

<220>
<223>  peptidase substrate

<220>
<221>  MISC_FEATURE
<222>  (1)..(1)
<223>  Modified with a detectable moiety

<220>
<221>  MISC_FEATURE
<222>  (6)..(6)
<223>  Modified with a detectable moiety

<400>  7
Ala Ala Arg Phe Ala Ala 
1               5       

<210>  8
<211>  6
<212>  PRT
<213>  artificial sequence

<220>
<223>  peptidase substrate

<220>
<221>  MISC_FEATURE
<222>  (1)..(1)
<223>  Modified with a detectable moiety

<220>
<221>  MISC_FEATURE
<222>  (6)..(6)
<223>  Modified with a detectable moiety

<400>  8
Ala Ala His Phe Ala Ala 
1               5       

<210>  9
<211>  6
<212>  PRT
<213>  artificial sequence

<220>
<223>  peptidase substrate

<220>
<221>  MISC_FEATURE
<222>  (1)..(1)
<223>  Modified with a detectable moiety

<220>
<221>  MISC_FEATURE
<222>  (6)..(6)
<223>  Modified with a detectable moiety

<400>  9
Ala Ala Leu Phe Ala Ala 
1               5       

<210>  10
<211>  6
<212>  PRT
<213>  artificial sequence

<220>
<223>  peptidase substrate

<220>
<221>  MISC_FEATURE
<222>  (1)..(1)
<223>  Modified with a detectable moiety

<220>
<221>  MISC_FEATURE
<222>  (6)..(6)
<223>  Modified with a detectable moiety

<400>  10
Ala Ala Tyr Phe Ala Ala 
1               5       

<210>  11
<211>  6
<212>  PRT
<213>  artificial sequence

<220>
<223>  peptidase substrate

<220>
<221>  MISC_FEATURE
<222>  (1)..(1)
<223>  Modified with a detectable moiety

<220>
<221>  MISC_FEATURE
<222>  (6)..(6)
<223>  Modified with a detectable moiety

<400>  11
Ala Ala Gln Phe Ala Ala 
1               5       

<210>  12
<211>  6
<212>  PRT
<213>  artificial sequence

<220>
<223>  peptidase substrate

<220>
<221>  MISC_FEATURE
<222>  (1)..(1)
<223>  Modified with a detectable moiety

<220>
<221>  MISC_FEATURE
<222>  (6)..(6)
<223>  Modified with a detectable moiety

<400>  12
Ala Ala Glu Phe Ala Ala 
1               5       

<210>  13
<211>  6
<212>  PRT
<213>  artificial sequence

<220>
<223>  peptidase substrate

<220>
<221>  MISC_FEATURE
<222>  (1)..(1)
<223>  Modified with a detectable moiety

<220>
<221>  MISC_FEATURE
<222>  (6)..(6)
<223>  Modified with a detectable moiety

<400>  13
Ala Ala Asp Phe Ala Ala 
1               5       

<210>  14
<211>  6
<212>  PRT
<213>  artificial sequence

<220>
<223>  peptidase substrate

<220>
<221>  MISC_FEATURE
<222>  (1)..(1)
<223>  Modified with a detectable moiety

<220>
<221>  MISC_FEATURE
<222>  (6)..(6)
<223>  Modified with a detectable moiety

<400>  14
Ala Ala Ala Phe Ala Ala 
1               5       

<210>  15
<211>  6
<212>  PRT
<213>  artificial sequence

<220>
<223>  peptidase substrate

<220>
<221>  MISC_FEATURE
<222>  (1)..(1)
<223>  Modified with a detectable moiety

<220>
<221>  MISC_FEATURE
<222>  (6)..(6)
<223>  Modified with a detectable moiety

<400>  15
Ala Ala Phe Phe Ala Ala 
1               5       

<210>  16
<211>  7
<212>  PRT
<213>  artificial sequence

<220>
<223>  peptidase substrate

<220>
<221>  MISC_FEATURE
<222>  (1)..(1)
<223>  Modified with a detectable moiety

<220>
<221>  MISC_FEATURE
<222>  (7)..(7)
<223>  Modified with a detectable moiety

<400>  16
Ala Ala Lys Phe Ala Ala Lys 
1               5           

<210>  17
<211>  7
<212>  PRT
<213>  artificial sequence

<220>
<223>  peptidase substrate

<220>
<221>  MISC_FEATURE
<222>  (1)..(1)
<223>  Modified with a detectable moiety

<220>
<221>  MISC_FEATURE
<222>  (7)..(7)
<223>  Modified with a detectable moiety

<400>  17
Ala Ala Phe Phe Ala Ala Lys 
1               5           

<210>  18
<211>  7
<212>  PRT
<213>  artificial sequence

<220>
<223>  peptidase substrate

<220>
<221>  MISC_FEATURE
<222>  (1)..(1)
<223>  Modified with a detectable moiety

<220>
<221>  MISC_FEATURE
<222>  (7)..(7)
<223>  Modified with a detectable moiety

<400>  18
Ala Ala Leu Phe Ala Ala Lys 
1               5           

