                         SEQUENCE LISTING

<110>  Arlow, Daniel
       Palluk, Sebastian
 
<120>  NUCLEIC ACID SYNTHESIS AND SEQUENCING USING TETHERED NUCLEOSIDE 
       TRIPHOSPHATES

<130>  BERK-339WO

<150>  62354635
<151>  2016-06-24

<160>  24    

<170>  PatentIn version 3.5

<210>  1
<211>  384
<212>  PRT
<213>  Tarsius syrichta

<400>  1

Met Gly His His His His His His His His His His Ser Ser Gly His 
1               5                   10                  15      


Ile Asp Asp Asp Asp Lys His Met Ser Gln Tyr Ala Cys Gln Arg Arg 
            20                  25                  30          


Thr Thr Leu Asn Asn His Asn Gln Ile Phe Thr Asp Ala Phe Asp Ile 
        35                  40                  45              


Leu Ala Glu Asn Asp Glu Phe Arg Glu Asn Glu Gly Pro Ser Leu Thr 
    50                  55                  60                  


Phe Met Arg Ala Ala Ser Val Leu Lys Ser Leu Pro Phe Thr Ile Ile 
65                  70                  75                  80  


Ser Met Lys Asp Ile Glu Gly Ile Pro Asn Leu Gly Asp Arg Val Lys 
                85                  90                  95      


Ser Ile Ile Glu Glu Ile Ile Glu Asp Gly Glu Ser Ser Ala Val Lys 
            100                 105                 110         


Ala Val Leu Asn Asp Glu Arg Tyr Lys Ser Phe Lys Leu Phe Thr Ser 
        115                 120                 125             


Val Phe Gly Val Gly Leu Lys Thr Ser Glu Lys Trp Phe Arg Met Gly 
    130                 135                 140                 


Phe Arg Thr Leu Ser Asn Ile Arg Ser Asp Lys Ser Leu Thr Phe Thr 
145                 150                 155                 160 


Arg Met Gln Arg Ala Gly Phe Leu Tyr Tyr Glu Asp Leu Val Ser Arg 
                165                 170                 175     


Val Thr Arg Ala Glu Ala Glu Ala Val Gly Val Leu Val Lys Glu Ala 
            180                 185                 190         


Val Trp Ala Ser Leu Pro Asp Ala Phe Val Thr Met Thr Gly Gly Phe 
        195                 200                 205             


Arg Arg Gly Lys Lys Thr Gly His Asp Val Asp Phe Leu Ile Thr Ser 
    210                 215                 220                 


Pro Gly Ala Thr Glu Glu Glu Glu Gln Gln Leu Leu His Lys Val Ile 
225                 230                 235                 240 


Ser Leu Trp Glu His Lys Gly Leu Leu Leu Tyr Tyr Asp Leu Val Glu 
                245                 250                 255     


Ser Thr Phe Glu Lys Leu Lys Leu Pro Ser Arg Lys Val Asp Ala Leu 
            260                 265                 270         


Asp His Phe Gln Lys Cys Phe Leu Ile Leu Lys Leu His His Gln Arg 
        275                 280                 285             


Val Asp Ser Asp Gln Ser Ser Trp Gln Glu Gly Lys Thr Trp Lys Ala 
    290                 295                 300                 


Ile Arg Val Asp Leu Val Val Cys Pro Tyr Glu Arg Arg Ala Phe Ala 
305                 310                 315                 320 


Leu Leu Gly Trp Thr Gly Ser Arg Gln Phe Glu Arg Asp Leu Arg Arg 
                325                 330                 335     


Tyr Ala Thr His Glu Arg Lys Met Ile Ile Asp Asn His Ala Leu Tyr 
            340                 345                 350         


Asp Lys Thr Lys Arg Ile Phe Leu Glu Ala Glu Ser Glu Glu Glu Ile 
        355                 360                 365             


Phe Ala His Leu Gly Leu Asp Tyr Ile Glu Pro Trp Glu Arg Asn Ala 
    370                 375                 380                 


<210>  2
<211>  807
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic sequence

<400>  2

Met Gly His His His His His His His His His His Ser Ser Gly His 
1               5                   10                  15      


Ile Asp Asp Asp Asp Lys His Met Met Lys Ile Glu Glu Gly Lys Leu 
            20                  25                  30          


Val Ile Trp Ile Asn Gly Asp Lys Gly Tyr Asn Gly Leu Ala Glu Val 
        35                  40                  45              


Gly Lys Lys Phe Glu Lys Asp Thr Gly Ile Lys Val Thr Val Glu His 
    50                  55                  60                  


Pro Asp Lys Leu Glu Glu Lys Phe Pro Gln Val Ala Ala Thr Gly Asp 
65                  70                  75                  80  


Gly Pro Asp Ile Ile Phe Trp Ala His Asp Arg Phe Gly Gly Tyr Ala 
                85                  90                  95      


Gln Ser Gly Leu Leu Ala Glu Ile Thr Pro Asp Lys Ala Phe Gln Asp 
            100                 105                 110         


Lys Leu Tyr Pro Phe Thr Trp Asp Ala Val Arg Tyr Asn Gly Lys Leu 
        115                 120                 125             


Ile Ala Tyr Pro Ile Ala Val Glu Ala Leu Ser Leu Ile Tyr Asn Lys 
    130                 135                 140                 


Asp Leu Leu Pro Asn Pro Pro Lys Thr Trp Glu Glu Ile Pro Ala Leu 
145                 150                 155                 160 


Asp Lys Glu Leu Lys Ala Lys Gly Lys Ser Ala Leu Met Phe Asn Leu 
                165                 170                 175     


Gln Glu Pro Tyr Phe Thr Trp Pro Leu Ile Ala Ala Asp Gly Gly Tyr 
            180                 185                 190         


Ala Phe Lys Tyr Glu Asn Gly Lys Tyr Asp Ile Lys Asp Val Gly Val 
        195                 200                 205             


Asp Asn Ala Gly Ala Lys Ala Gly Leu Thr Phe Leu Val Asp Leu Ile 
    210                 215                 220                 


Lys Asn Lys His Met Asn Ala Asp Thr Asp Tyr Ser Ile Ala Glu Ala 
225                 230                 235                 240 


Ala Phe Asn Lys Gly Glu Thr Ala Met Thr Ile Asn Gly Pro Trp Ala 
                245                 250                 255     


Trp Ser Asn Ile Asp Thr Ser Lys Val Asn Tyr Gly Val Thr Val Leu 
            260                 265                 270         


Pro Thr Phe Lys Gly Gln Pro Ser Lys Pro Phe Val Gly Val Leu Ser 
        275                 280                 285             


Ala Gly Ile Asn Ala Ala Ser Pro Asn Lys Glu Leu Ala Lys Glu Phe 
    290                 295                 300                 


Leu Glu Asn Tyr Leu Leu Thr Asp Glu Gly Leu Glu Ala Val Asn Lys 
305                 310                 315                 320 


Asp Lys Pro Leu Gly Ala Val Ala Leu Lys Ser Tyr Glu Glu Glu Leu 
                325                 330                 335     


Val Lys Asp Pro Arg Ile Ala Ala Thr Met Glu Asn Ala Gln Lys Gly 
            340                 345                 350         


Glu Ile Met Pro Asn Ile Pro Gln Met Ser Ala Phe Trp Tyr Ala Val 
        355                 360                 365             


Arg Thr Ala Val Ile Asn Ala Ala Ser Gly Arg Gln Thr Val Asp Glu 
    370                 375                 380                 


Ala Leu Lys Asp Ala Gln Thr Asn Ser Ser Ser Asn Asn Asn Asn Asn 
385                 390                 395                 400 


Asn Asn Asn Asn Asn Leu Gly Ile Glu Gly Arg Ile Ser His Met Ser 
                405                 410                 415     


Met Gly Gly Arg Asp Ile Val Asp Gly Ser Glu Phe Ser Pro Ser Pro 
            420                 425                 430         


Val Pro Gly Ser Gln Asn Val Pro Ala Pro Ala Val Lys Lys Ile Ser 
        435                 440                 445             


Gln Tyr Ala Cys Gln Arg Arg Thr Thr Leu Asn Asn Tyr Asn Gln Leu 
    450                 455                 460                 


Phe Thr Asp Ala Leu Asp Ile Leu Ala Glu Asn Asp Glu Leu Arg Glu 
465                 470                 475                 480 


Asn Glu Gly Ser Ala Leu Ala Phe Met Arg Ala Ser Ser Val Leu Lys 
                485                 490                 495     


Ser Leu Pro Phe Pro Ile Thr Ser Met Lys Asp Thr Glu Gly Ile Pro 
            500                 505                 510         


Ser Leu Gly Asp Lys Val Lys Ser Ile Ile Glu Gly Ile Ile Glu Asp 
        515                 520                 525             


Gly Glu Ser Ser Glu Ala Lys Ala Val Leu Asn Asp Glu Arg Tyr Lys 
    530                 535                 540                 


Ser Phe Lys Leu Phe Thr Ser Val Phe Gly Val Gly Leu Lys Thr Ala 
545                 550                 555                 560 


Glu Lys Trp Phe Arg Met Gly Phe Arg Thr Leu Ser Lys Ile Gln Ser 
                565                 570                 575     


Asp Lys Ser Leu Arg Phe Thr Gln Met Gln Lys Ala Gly Phe Leu Tyr 
            580                 585                 590         


Tyr Glu Asp Leu Val Ser Cys Val Asn Arg Pro Glu Ala Glu Ala Val 
        595                 600                 605             


Ser Met Leu Val Lys Glu Ala Val Val Thr Phe Leu Pro Asp Ala Leu 
    610                 615                 620                 


Val Thr Met Thr Gly Gly Phe Arg Arg Gly Lys Met Thr Gly His Asp 
625                 630                 635                 640 


Val Asp Phe Leu Ile Thr Ser Pro Glu Ala Thr Glu Asp Glu Glu Gln 
                645                 650                 655     


Gln Leu Leu His Lys Val Thr Asp Phe Trp Lys Gln Gln Gly Leu Leu 
            660                 665                 670         


Leu Tyr Ala Asp Ile Leu Glu Ser Thr Phe Glu Lys Phe Lys Gln Pro 
        675                 680                 685             


Ser Arg Lys Val Asp Ala Leu Asp His Phe Gln Lys Cys Phe Leu Ile 
    690                 695                 700                 


Leu Lys Leu Asp His Gly Arg Val His Ser Glu Lys Ser Gly Gln Gln 
705                 710                 715                 720 


Glu Gly Lys Gly Trp Lys Ala Ile Arg Val Asp Leu Val Met Ser Pro 
                725                 730                 735     


Tyr Asp Arg Arg Ala Phe Ala Leu Leu Gly Trp Thr Gly Ser Arg Gln 
            740                 745                 750         


Phe Glu Arg Asp Leu Arg Arg Tyr Ala Thr His Glu Arg Lys Met Met 
        755                 760                 765             


Leu Asp Asn His Ala Leu Tyr Asp Arg Thr Lys Arg Val Phe Leu Glu 
    770                 775                 780                 


Ala Glu Ser Glu Glu Glu Ile Phe Ala His Leu Gly Leu Asp Tyr Ile 
785                 790                 795                 800 


Glu Pro Trp Glu Arg Asn Ala 
                805         


<210>  3
<211>  46
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Syntthetic sequence


<220>
<221>  Misc_feature
<222>  (1)..(1)
<223>  5' phosphorylation at the 5' end

<220>
<221>  Misc_feature
<222>  (1)..(1)
<223>  n is a uracil

<220>
<221>  Misc_feature
<222>  (22)..(22)
<223>  n is a deoxythymidine nucleotide base modified with fluorescein

<400>  3
ntgaagagcg agagtgagtg ancattaaag acgtgggcct ggattt                      46


<210>  4
<211>  59
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic sequence

<400>  4
gtgccgtgag acctggctcc tgacgatatg gataagcttt gaagagcgag agtgagtga        59


<210>  5
<211>  56
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Syntthetic sequence

<400>  5
aaaagaattc aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaa           56


<210>  6
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Syntthetic sequence


<220>
<221>  Misc_feature
<222>  (1)..(1)
<223>  phosphorylated at the 5' end

<400>  6
cattaaagac gtgggcgtgg a                                                 21


<210>  7
<211>  26
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Syntthetic sequence


<220>
<221>  Misc_feature
<222>  (4)..(4)
<223>  n is a internal amino group labeled with Cy3 NHS

<400>  7
tttntgtgaa atccttccct cgatcc                                            26


<210>  8
<211>  42
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic sequence


<220>
<221>  Misc_feature
<222>  (1)..(1)
<223>  aminohexyl modification of a 5' phosphate at the 5' end

<400>  8
cgactcacct cacgtcctca tgtgaaatcc ttccctcgat cc                          42


<210>  9
<211>  30
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Syntthetic sequence

<400>  9
ttttgaattc caacacacca cccacccaac                                        30


<210>  10
<211>  30
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Syntthetic sequence

<400>  10
ttttaagctt cgactcacct cacgtcctca                                        30


<210>  11
<211>  47
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Syntthetic sequence


<220>
<221>  Misc_feature
<222>  (1)..(1)
<223>  5' phosphorylation at the 5' end

<400>  11
gcagccaact cagcttctgc aggggctttg ttagcagccg gatcctc                     47


<210>  12
<211>  49
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Syntthetic sequence

<400>  12
aaacaagcgc tcatgagcca gaaatctgga gcccgatctt ccccatcgg                   49


<210>  13
<211>  60
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Syntthetic sequence

<400>  13
gtgccgtgag acctggctcc tgacgaggat aagcttctat agtgagtcgt attaatttcg       60


<210>  14
<211>  40
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Syntthetic sequence


<220>
<221>  Misc_feature
<222>  (1)..(1)
<223>  aminohexyl modification of a 5' phosphate at the 5' end

<400>  14
caacacacca cccacccaac cgcagatgtc cgctttctgg                             40


<210>  15
<211>  11
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic sequence


<220>
<221>  Misc_Feature
<222>  (1)..(1)
<223>  linked to a -C tripple bond CCH2NH2

<220>
<221>  Misc_Feature
<222>  (2)..(2)
<223>  linked to a -C tripple bond CCH2NH2

<220>
<221>  Misc_Feature
<222>  (3)..(3)
<223>  linked to a -C tripple bond CCH2NH2

<220>
<221>  Misc_Feature
<222>  (4)..(4)
<223>  linked to a -C tripple bond CCH2NH2

<220>
<221>  Misc_Feature
<222>  (11)..(11)
<223>  OH group at the 3' end

<400>  15
ctagtttttt t                                                            11


<210>  16
<211>  11
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic sequence


<220>
<221>  Misc_feature
<222>  (11)..(11)
<223>  OH group at the 3' end

<400>  16
ctagtttttt t                                                            11


<210>  17
<211>  11
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic sequence

<400>  17
tttctagttt t                                                            11


<210>  18
<211>  10
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic sequence

<400>  18
ctactgactg                                                              10


<210>  19
<211>  10
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic sequence


<220>
<221>  Misc_feature
<222>  (1)..(1)
<223>  linked to a -C tripple bond CCH2NH2

<220>
<221>  Misc_feature
<222>  (1)..(1)

<220>
<221>  Misc_feature
<222>  (2)..(2)
<223>  linked to a -C tripple bond CCH2NH2

<220>
<221>  Misc_feature
<222>  (3)..(3)
<223>  linked to a -C tripple bond CCH2NH2

<220>
<221>  Misc_feature
<222>  (4)..(4)
<223>  linked to a -C tripple bond CCH2NH2

<220>
<221>  Misc_feature
<222>  (5)..(5)
<223>  linked to a -C tripple bond CCH2NH2

<220>
<221>  Misc_feature
<222>  (6)..(6)
<223>  linked to a -C tripple bond CCH2NH2

<220>
<221>  Misc_feature
<222>  (7)..(7)
<223>  linked to a -C tripple bond CCH2NH2

<220>
<221>  Misc_feature
<222>  (8)..(8)
<223>  linked to a -C tripple bond CCH2NH2

<220>
<221>  Misc_feature
<222>  (9)..(9)
<223>  linked to a -C tripple bond CCH2NH2

<220>
<221>  Misc_feature
<222>  (10)..(10)
<223>  OH group at the 3' end

<220>
<221>  Misc_feature
<222>  (10)..(10)
<223>  linked to a -C tripple bond CCH2NH2

<400>  19
ctactgactg                                                              10


<210>  20
<211>  17
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic sequence


<220>
<221>  Misc_feature
<222>  (1)..(1)
<223>  linked to a -C tripple bond CCH2NH2

<220>
<221>  Misc_feature
<222>  (2)..(2)
<223>  linked to a -C tripple bond CCH2NH2

<220>
<221>  Misc_feature
<222>  (3)..(3)
<223>  linked to a -C tripple bond CCH2NH2

<220>
<221>  Misc_feature
<222>  (4)..(4)
<223>  linked to a -C tripple bond CCH2NH2

<220>
<221>  Misc_feature
<222>  (5)..(5)
<223>  linked to a -C tripple bond CCH2NH2

<220>
<221>  Misc_feature
<222>  (6)..(6)
<223>  linked to a -C tripple bond CCH2NH2

<220>
<221>  Misc_feature
<222>  (7)..(7)
<223>  linked to a -C tripple bond CCH2NH2

<220>
<221>  Misc_feature
<222>  (8)..(8)
<223>  linked to a -C tripple bond CCH2NH2

<220>
<221>  Misc_feature
<222>  (9)..(9)
<223>  linked to a -C tripple bond CCH2NH2

<220>
<221>  Misc_feature
<222>  (10)..(10)
<223>  linked to a -C tripple bond CCH2NH2

<220>
<221>  Misc_feature
<222>  (17)..(17)
<223>  OH group at the 3' end

<400>  20
ctactgactg ttttttt                                                      17


<210>  21
<211>  17
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic sequence

<400>  21
ctactgactg ttttttt                                                      17


<210>  22
<211>  17
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic sequence

<400>  22
tttctactga ctgtttt                                                      17


<210>  23
<211>  11
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic sequence

<400>  23
aaaaaaacta g                                                            11


<210>  24
<211>  17
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic sequence

<400>  24
aaaaaaacag tcagtag                                                      17


